Capillary Density Analysis
Contents
1. CapiScope User Manual KK Research Technology Capillary Density Analysis Analysing Video 1 Open the video file 2 Press the lower play button or drag the horizontal scrollbar to the first measurement site and find a Swans ie ASCHampSte K eR Ewe oY 3 Select menu edit duplicate image in new window Sve ee Om e VREwe Gee 4 Click on the tools button at the bottom of the right hand toolbar to open up the image processing toolbar buttons es Status BapaGte Bs oH aM AG Eg af m 5 Click on the Equalise button to enhance the contrast of the duplicated window Page 1 3 Issue 27 02 2013 22 08 17 CapiScope User Manual KK Research Technology 6 Click on the measurement button to open up the dimension measurement buttons a at Geb Bao ns TERRAE NE CEES icy Sao 7 Press toolbar button find capillaries 8 Check the resultant located capillaries 9 If there are any false small capillaries its possible to alter the minimum and maximum detection thresholds Open the dimension list window CapiScope amp CAM1 Image7 S Live Video Video Recorder CAM1 Monitor Grey level Linescan Dimension list PSD profile Chart Results 1 mid phalange lowpower des v 2Image7 11 Use menu Settings Processing to open the processing settings dialog window 12 And enter the2. minimum area Delete the duplicated image window and go back to step 2 G capiscope amp CAM1 Image7 Fie Edit View Settings Controls Tools Window Help Select camera negative window size for lowpass Ee smoothing and positive window size Window 1 lor T s sharpen or zero for no window 2 jio SAN v gpn b AAHAAHHAAAAAAAAAH AAA AR a NNN BAR Page 2 3 Issue 27 02 2013 22 08 17 CapiScope User Manual KK Research Technology 13 Delete any false positives by clicking on the first node the cross by the dimension number to select it then press the Delete key In this example three capillaries have been detected as a single large capillary 14 Use the toolbar button counting and click on any missed capillaries URIs line2 985 1 15 Press key or the toolbar button to get the Dim count density 425 24 7 76 0 00 0 0000 2 30 7 76 0 00 0 0000 755 98 7 76 0 00 0 0000 562 17 7 76 0 00 0 0000 483 52 7 76 0 00 0 0000 664 44 7 76 0 00 0 0000 717 11 7 76 TEn 0 0000 755 98 7 76 wi 578 80 0 0 0 0 0 0 0 0 Linear dens 3 360 Area density 113 4 15 26 16 Use the play or scrollbar to find the next 3 measurement areas and repeat steps 3 15 for each one 17 Save the image results window and or select copy and paste the dimension count density results into Excel or other spreadsheet 18 Save the indi
3. vidual measurement images if required To manually count capillaries miss out steps 7 13 these steps only need to be done once each time CapiScope is opened this only needs to be once Settings will be saved in the settings profile Page 3 3 Issue 27 02 2013 22 08 17
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