SUB Series Horizontal Electrophoresis Units
Contents
1. piii instrument Les dissolvants organiques causeront des dommages irr parables a l unit Ne pas fonctionner avec les temp ratures de tampon au dessus du maximum a sp cifi des sp cifications techniques La surchauffe causera des dommages irr parables l unit Wichtige Informationen German Wenn diese Ausr stung gewisserma en nicht angegeben durch Hoefer Inc verwendet wird kann der durch die Ausr stung zur Verf gung gestellte Schutz verschlechtert werden Dieses Instrument wird f r den Innenlaborgebrauch nur daf r entworfen Nur Zus tze und Teile genehmigten oder lieferten durch Hoefer Inc kann f r das Funktionieren das Aufrechterhalten und die Wartung dieses Produktes verwendet werden Verwenden Sie nur eine Energieversorgung die CE gekennzeichnet oder durch ein national anerkanntes Probelaboratorium bescheinigte Sicherheit ist Der Sicherheitsdeckel muss im Platz vor dem AnschlieBen der Energieversorgung sein f hrt zu einer Energieversorgung Alle Energieversorgungssteuerungen abdrehen und die Macht trennen f hrt vor dem Entfernen des Sicherheitsdeckels Nur Wasser oder 50 50 Glykol des Wassers Athylens durch den W rmeaustauscher wenn so ausgestattet in Umlauf setzen Verbinden Sie den W rmeaustauscher mit einem Wasserklaps oder jeder K hlmittel Quelle nicht wo der Wasserdruck ungeregelt wird F hren Sie nie Frostschutzmittel oder jedes organische L su2. manual SUB Series Horizontal Electrophoresis Units SUB6 SUB10 SUB13 SUB15 SUB20 SUB25 SUB20C SUB25C and SUBHT um SUB IM Rev CO 07 12 A Hoefer Page Finder Important INFORMA ON ss acariciar ii Waste Electrical amp Electronic Equipment WEEE ix Specifications cantada AAAA 1 Safety Precautions General Care and Maintenance eee 3 Gel Loading Instructions for the SUBHT 5 Cleaning and Storage ccccocncnnccnonncncnonncnnnnnccnnnnnnns 6 Packing Listos 7 Operational asii 9 SUBG Mini Horizontal nennen 9 SUB10 Mini Plus Horizontal 10 SUB13 Midi Horizontal 11 SUB15 Standard Horizontal 1 12 SUB20 Maxi Standard Horizontal 13 SUB25 Maxi Plus Standard Horizontal 14 SUB20C Maxi Cooled Horizontal 15 SUB25C Maxi Plus Cooled Horizontal 16 SUBHT High Throughput Horizontal 17 Using The Horizontal Gel Electrophoresis Units 18 Preparation of Agarose Gels 18 Agarose Gel Electrophoresis 19 Gel GUTE 20 Rurining the Gel toes see n see dess savana 22 Important Information English If this equipment is used in a manner not specified by Hoefer Inc the protection provided by the equipment may be impaired This instrument is designed for indoor laboratory use only Only acce
3. Technical Specifications Unit dimensions W x L x H 21 5 x 33 5 x 7 cm Gel size W x L 15x 15cm Buffer volume 1200 ml Buffer re circulation ports 2 Maximum sample capacity 120 Combs 2 Comb thickness 1 1 5 or 2 mm Comb throughput 1 to 30 samples Comb slots 4 Migration distance between comb slots 3 5 cm Recommended running voltage 100 to 125V Max Sample Volume Thickness Sample Tooth Width Spacing in a 5 mm Catalog No mm Throughput mm mm deep well ul SUB15 C1 10 1 10 12 8 2 64 SUB15 C1 16MC 1 16 6 4 2 32 SUB15 C1 20 1 20 5 9 2 29 SUB15 C1 25 oi 25 35 2 17 SUB15 C1 30MC 1 30 2 5 2 12 SUB15 C1 5 10 1 5 10 12 8 2 96 SUB15 C1 5 16MC 1 5 16 6 4 2 48 SUB15 C1 5 20 1 5 20 5 9 2 43 SUB15 C1 5 25 1 5 25 3 5 2 25 SUB15 C1 5 30MC 1 5 30 2 5 2 18 SUB15 C2 10 2 10 12 8 2 128 SUB15 C2 16MC 2 16 6 4 2 64 SUB15 C2 20 2 20 5 9 2 58 SUB15 C2 25 2 25 3 5 2 34 SUB15 C2 30MC 2 30 2 5 2 24 Multi channel pipette compatible e p12 SUB20 Maxi Standard Horizontal The SUB20 Maxi Standard Horizontal gel electrophoresis unit is ideal for high resolution analytical and preparative studies of nucleic acids E M D Technical Specifications Unit dimensions W x L x H 27 x 47 5 x 8 cm Gel size W x L 20 x 20cm Buffer volume 2200 ml Buffer re circulation ports 2 Maximum sample capacity 160 Combs 2 Comb t
4. 1 Gel casting tray SUB25 UT 2 Casting gates silicone seals SUB25 CG 6 Combs 1 0 mm 26 sample SUB25 C1 26MC 2 Buffer recirculation ports SUB BRP 6 Colored loading strips SUB25 CS SUB20C 1 Running tank lid 1 Gel casting tray SUB20 UT 2 Casting gates silicone seals SUB20 CG 2 Combs 1 0 mm 16 sample SUB20 C1 16 2 Buffer recirculation ports SUB BRP 6 Colored loading strips SUB20 CS SUB25C 1 Running tank lid 1 Gel casting tray SUB25 UT 2 Casting gates silicone seals SUB25 CG 6 Combs 1 0 mm 26 sample SUB25 C1 26MC 2 Buffer recirculation ports SUB BRP 6 Colored loading strips SUB25 CS SUBHT 1 Running tank lid 1 Labeled gel casting tray SUBHT LUT 2 Casting gates silicone seals SUBHT CG 2 Combs 1 0 mm 30 sample SUB15 C1 30MC 2 Buffer recirculation ports SUB BRP 6 Colored loading strips SUBHT CS Operational SUB6 Mini Horizontal The SUB6 Mini Horizontal gel electrophoresis unit is ideal for routine preparatory and analytical electrophoresis techniques Technical Specifications Unit dimensions W x L x H 13 x 24 x 6 5 cm Gel dimensions W x L 6 x 7 5 cm Buffer volume 325 ml Maximum sample capacity 32 Combs 2 Comb thickness 1 1 50r2mm Comb throughput 8 to 16 samples Comb slots 2 Migration distance between comb slots 3 5cm Recommended running voltage 70 to 90V SUB6 Combs Max Sample Volume Thickness Sample Tooth Width Spacing in a 5 mm Catalog No mm Throughput
5. loading This aids sinking of the sample into the well and visualization of how far the samples have run Consult your laboratory manual for details on loading buffers Load the samples into the wells taking care not to damage the sides or bottoms of the wells Replace the lid correctly BEFORE connecting the leads to the power supply Set the voltage and current to suit the electrophoretic application As a guide to obtain the optimum resolution of DNA fragments agarose gels should not be run greater than 5V cm Long runs may require buffer re circulation to prevent over heating and or buffer depletion Re circulation ports are provided in the SUB13 SUB15 SUB20 SUB20C SUB25 SUB25C and SUBHT units The Cooled gel units have a built in cooling block in the base The side connectors can be attached via tubing to the mains water supply or to a circulating water bath Hoefer Inc 84 October Hill Road Holliston MA 01746 Toll Free 1 800 227 4750 Phone 1 508 893 8999 Fax 1 508 893 0176 E mail support hoeferinc com Web www hoeferinc com Hoefer is a registered trademark of Hoefer Inc O 2012 Hoefer Inc All rights reserved Printed in the USA Hoefer
6. mm mm deep well ul SUB6 C1 8 1 8 4 5 2 20 SUB6 C1 12MC 1 2 2 5 2 11 SUB6 C1 16 1 16 2 2 1 10 SUB6 C1 5 8 1 5 8 4 5 2 30 SUB6 C1 5 12MC 1 5 2 2 5 2 17 SUB6 C1 5 16 1 5 16 2 2 1 15 SUB6 C2 8 2 8 4 5 2 40 SUB6 C2 12MC 2 2 2 5 2 22 SUB6 C2 16 2 16 2 2 1 20 Multi channel pipette compatible SUB10 Mini Plus Horizontal The SUB10 Mini Plus Horizontal gel electrophoresis unit is ideal for routine preparatory and analytical electrophoresis techniques Technical Specifications Unit dimensions W x L x H 16 5 x 23 x 6 5cm Gel dimensions W x L 10x 11 5cm Buffer volume 450 ml Maximum sample capacity 80 Combs 2 Comb thickness 1 1 5 or 2 mm Comb throughput 8 to 20 samples Comb slots 4 Migration distance between comb slots 2 5 cm Recommended running voltage 75 to 125V SUB10 Combs Max Sample Volume Thickness Sample Tooth Width Spacing in a 5 mm Catalog No mm Throughput mm mm deep well ul SUB10 C1 10MC 1 10 7 2 30 SUB10 C1 12 1 12 5 5 2 25 SUB10 C1 16 1 16 3 6 2 15 SUB10 C1 20MC 1 20 3 1 5 12 SUB10 C1 5 8 1 5 8 9 2 5 60 SUB10 C1 5 10MC 1 5 10 7 2 45 SUB10 C1 5 12 1 5 12 5 5 2 35 SUB10 C1 5 16 1 5 16 3 6 2 25 SUB10 C1 5 20MC 1 5 20 3 1 5 20 SUB10 C2 8 2 8 9 2 5 80 SUB10 C2 10MC 2 10 7 2 60 SUB10 C2 12 2 12 5 5 2 50 SUB10 C2 16 2 16 3 6 2 30 SUB10 C2 20MC 2 20 3 1 5 25 Multi channel pipette compatible e plo SU
7. B13 Midi Horizontal The SUB13 Midi Horizontal gel electrophoresis unit is ideal for analytical and preparative studies of nucleic acids A l T Technical Specifications D Z K Unit dimensions W x L x H 20 x 32x 7 cm SE Gel dimensions W x L 2 8x 15cm Buffer volume 900 ml Buffer re circulation ports 2 Maximum sample capacity 112 Combs 2 Comb thickness 1 1 5 or 2 mm Comb throughput 10 to 28 samples Comb slots 4 Migration distance between comb slots 3 5 cm Recommended running voltage 100 to 125 V SUB13 Combs Max Sample Volume Thickness Sample Tooth Width Spacing in a 5 mm Catalog No mm Throughput mm mm deep well ul SUB13 C1 10 1 10 9 5 2 5 40 SUB13 C1 12MC 1 12 8 2 35 SUB13 C1 16 1 16 5 5 2 25 SUB13 C1 20 1 20 4 2 17 SUB13 C1 24 1 24 3 2 13 SUB13 C1 28MC 1 28 3 1 5 13 SUB13 C1 5 10 1 5 10 9 5 2 5 60 SUB13 C1 5 12MC 1 5 12 8 2 50 SUB13 C1 5 16 1 5 16 5 5 2 35 SUB13 C1 5 20 1 5 20 4 2 25 SUB13 C1 5 24 15 24 3 2 20 SUB13 C1 5 28MC 1 5 28 3 1 5 20 SUB13 C2 10 2 10 9 5 2 5 85 SUB13 C2 12MC 2 12 8 2 70 SUB13 C2 16 2 16 5 5 2 50 SUB13 C2 20 2 20 4 2 35 SUB13 C2 24 2 24 3 2 25 SUB13 C2 28MC 2 28 3 1 5 25 Multi channel pipette compatible e pll SUB15 Combs SUB15 Standard Horizontal The SUB15 Standard Horizontal gel electrophoresis unit provides the comb throughput and tray size options most popular in today s market
8. B25 C2 26MC 2 26 7 2 60 SUB25 C2 52MC 2 52 3 1 5 25 Multi channel pipette compatible e pl4 SUB20C Combs SUB20C Maxi Cooled Horizontal The SUB20C Maxi Cooled Horizontal gel electrophoresis unit allows analytical and preparative studies of nucleic acids to be performed quickly at high voltage without compromising resolution Technical Specifications Unit dimensions W x L x H Gel size W x L Buffer volume Buffer re circulation ports Maximum sample capacity Combs Comb thickness Comb throughput Comb slots Cooling ports Migration distance between comb slots Recommended running voltage 27 x 47 5 x 8 cm 20 x 20 cm 2200 ml 2 160 2 1 1 50r2mm 16 to 40 samples 6 2 5 cm 150 to 225V Max Sample Volume Thickness Sample Tooth Width Spacing in a 5 mm Catalog No mm Throughput mm mm deep well ul SUB20 C1 16 1 16 8 5 3 35 SUB20 C1 20MC 1 20 7 2 30 SUB20 C1 28 1 28 2 20 SUB20 C1 40MC 1 40 2 T3 SUB20 C1 5 16 1 5 16 8 5 3 55 SUB20 C1 5 20MC 1 5 20 7 2 45 SUB20 C1 5 28 1 5 28 2 30 SUB20 C1 5 40MC 1 5 40 2 19 SUB20 C2 16 2 16 8 5 3 75 SUB20 C2 20MC 2 20 7 2 60 SUB20 C2 28 2 28 2 40 SUB20 C2 40MC 2 40 2 25 Multi channel pipette compatible e p15 SUB25C Combs SUB25C Maxi Plus Cooled Horizontal The SUB25C Maxi Plus Cooled Horizontal gel electrophoresis unit allows high throughput screening of nucleic acids to be performed quickly at high voltage without compromising reso
9. D the instructions before using the apparatus e The exclamation mark within an equilateral triangle is intended to alert the user to the presence of important operating and maintenance instructions in the literature accompanying the instrument Always unplug electrophoresis units from their power supply before removing the safety cover Unplug the power supply from the mains FIRST then disconnect the leads DO NOT exceed the maximum operating voltage or current DO NOT operate the electrophoresis units in metal trays It is advisable to always place the unit on a plastic safety tray during electrophoresis Ethidium bromide is a known mutagen Wear effective protective clothing when handling and follow recommended handling and disposal procedures Ultraviolet light can cause permanent damage to the retina and skin cancer Wear UV safety goggles and cover any exposed skin when using a UV light source Following the replacement of a platinum electrode have the unit inspected and approved by your safety officer prior to use DO NOT fill the unit with running buffer above the maximum fill lines DO NOT move the unit when it is running CAUTION During electrophoresis very low quantities of various gases are produced at the electrodes The type of gas produced depends on the composition of the buffer employed To disperse these gases make sure that the apparatus is run in a well ventilated area IMPORTANT Acrylic plast
10. Informac es Importantes Portuguese Se este equipamento usado numa maneira n o especificada por Hoefer Inc que a protecc o fornecida pelo equipamento pode ser comprometida Este instrumento projectado para uso de interior de laborat rio s S acess rios e partes aprovaram ou forneceu por Hoefer Inc pode ser usada para operar manter e servicing este produto S usa um estoque de poder que CE marcou ou seguranca registrada por um nacionalmente reconhecido testando laborat rio A tampa de seguranca deve estar em lugar antes de ligar o estoque de poder leva a um estoque de poder Desliga todos controlos de estoque de poder e desconecta os chumbos de poder antes de retirar a tampa de seguranca Circulam s gua ou 50 50 glicol de gua ethylene pelo exchanger de calor se for assim equiparam N o ligue o exchanger de calor a uma torneira de gua nem qualquer fonte de refrigerante onde a press o de gua n o regulado Nunca introduz anticongelante nem qualquer org nico solvente em qualquer parte do instrumento Org nico solvente causar agress o irrepar vel unidade N o opera com temperaturas de buffer acima do m ximo especificou especificac es t cnicas Superaquecer causar agress o irrepar vel a unidade e pv Informaci n Importante Spanish Si este equipo es utilizado en una manera no especificado por Hoefer Inc la protecci n proporcionado por el equipo puede
11. asjoner A overoppheting vil for rsake irreparabel skade p enheten Wazne Informacje Polish Je eli ten sprz t jest wykorzystywany w spos b nie okre lone przez Hoefer Inc do ochrony przewidzianej przez urz dzenie mo e zosta obni ony Instrument ten jest przeznaczony do u ytku w laboratoriach kryty tylko Tylko akcesori w i cz ci zatwierdzone lub dostarczone przez Hoefer Inc mog by wykorzystane do eksploatacji utrzymania i obs ugi tego produktu korzysta jedynie zasilacza e jest nosz ce oznakowanie CE lub bezpiecze stwa uwierzytelnione przez uznane na poziomie krajowym laboratorium badawcze Bezpiecze stwo lid musi by w miejsce przed pod czeniem zasilania prowadzi do zasilania Za wszystkie r d a zasilania urz dzenia steruj ce off i od czy moc prowadzi przed odbiorem bezpiecze stwa lid Kr tylko wody lub wody 50 50 ethylene glycol wymiennik ciep a poprzez je li tak wyposa one Nie nale y po czy wymiennik ciep a woda z kranu lub jakimkolwiek ch odziwo r d a je eli ci nienie wody jest nieuregulowanych Nigdy nie wprowadza rozpuszczalnika organicznego przeciw zamarzaniu lub jakichkolwiek na dowoln cz dokumentu Rozpuszczalniki organiczne spowoduje nieodwracalne szkody dla jednostki Nie dzia aj w buforze temperatury powy ej maksymalnego okre lone specyfikacje techniczne Przegrzania spowoduje nieodwracalne szkody dla jednostki
12. askets facing outwards Place the gel unit on a level surface Position the required combs into the slots provided in the gel casting tray Prepare 115 200 or 375 mls of the desired agarose for units SUB15 SUBHT SUB20 SUB20C or SUB25 SUB25C respectively This will give a gel depth of 5 mm For a 10 mm deep gel prepare 230 400 or 750 mls respectively Using a 1 ml disposable pipette seal the inside of the casting gates with some of the cooled agarose Pour in the agarose smoothly so as not to gener ate bubbles If bubbles do occur these can be smoothed to the side of the gel and dispersed using a clean gloved hand Allow the agarose to set ensuring that the gel remains undisturbed Carefully remove the gel casting gates and comb s Place the gel casting tray in the running position such that the wells are nearest to the black elec trode cathode Fill the tank with running buffer so that the buffer just submerges the gel e p21 IMPORTANT Do not exceed the recommended voltage or current as this may result in poor band resolution and may result in damage to the unit IMPORTANT When re circulating buffer remember that the buffer flowing through the tubing is live Take all necessary precautions Warn other workers in the vicinity of the potential hazard Seek the advice of your safety officer e p22 Running the Gel I Samples should be mixed with a suitable marker dye or loading buffer prior to
13. bn mi laborato Bezpe nosti lid mus b t zavedena p ed p ipojen m nap jec zdroj nap jen vede k Turn ve ker nap jen kontroly vypnuto a odpojit p ed odb rem energie vede bezpe nostn v ko Rozeslat pouze voda nebo 50 50 voda ethylenglykolu prost ednictv m v m n k tepla je li to vybavena Nemaj p ipojen v m n k tepla s vodn mi set epn nebo jak koli chladic kapaliny zdroje kde tlak vody je neregulo Nikdy zav st prost edek proti zamrznut nebo jak koli organick rozpou t dla do jak koli sti z tohoto n stroje Rozpustidl m zp sob nenapraviteln po kozen jednotka Nejsou provozov na s pufru teplot ch nad maxim ln stanovenou technick mi specifikacemi P eh t zp sob nenapraviteln po kozen jednotka Vigtig Information Danish Hvis dette udstyr bruges i en made ikke specificeret ved Hoefer Inc den beskyttelse som er blevet forsynet af udstyret kan m ske svaekkes Dette instrument er designet for indend rs laboratoriumbrug bare Bare tilbehar og del godkendede eller forsynede ved Hoefer Inc kan maske bruges for drive funktionsfejl og betjening dette produkt bruger Bare en str mforsyning der er CE markerede eller sikkerhed som er blevet attesteret af en som nationalt er blevet anerkendt prove laboratorium Sikkerhedlaget m v re p plads for forbinding stramforsyningsblyet til en stramforsyning Drejer alle stram
14. dello strumento solventi organici causeranno il danno irreparabile all unit Non opera con le temperature di tampone al di sopra del massimo ha specificato le descrizioni tecniche Il surriscaldamento causer il danno irreparabile all unit Viktig Informasjon Norwegian Hvis dette utstyret blir brukt i en m te ikke spesifisert ved Hoefer Inc beskyttelsen som ha blitt git av utstyret kan bli svekket Dette instrumentet er utformet for innend rs laboratoriumbruk bare Bare tilbeh r og deler godkjente eller forsynte ved Hoefer Inc kan bli brukt for drive vedlikeholde og betjene dette produktet bruker Bare en kraftforsyning som er CE merket eller sikkerhet som ha blitt sertifisert av et som e piv nasjonalt ha blitt anerkjent prover laboratorium Sikkerheten lokket m veere p plass for forbinding kraftforsyningene blyene til en kraftforsyning Vender all kraftforsyningsstyring av og frakopler kreftene blyene for fjerning sikkerheten lokket Sirkulerer bare vann eller 50 50 vann ethylene glykol gjennom oppvarmingen veksleren i s fall utstyrer Ikke forbind oppvarmingen veksleren til en vanntapp eller noe kjalemiddelkilde hvor vannet trykket er unregulated Introduserer Aldri antifreeze eller noe organisk losemiddel inn i noe del av instrumentet Organiske losemiddler vil for rsake irreparabel skade pa enheten Driver med buffertemperaturer over maksimum ikke spesifiserte teknisk spesifik
15. e eller levererade vid Hoefer Inc kan anv ndas f r fungera underh lla och servicing denna produkt anv nder bara en kraft tillg ng som r CE markerade eller s kerhet intygade vid en nationellt erk nd testande laboratorium S kerheten locket m ste vara p platsen f re koppla kraften tillg ngen blyen till en kraft tillg ng V nder sig alla kraft tillg ng kontroller av och kopplar bort kraften blyen f re flytta s kerheten locket Cirkulerar bara vatten eller 50 50 vatten ethylene glycol genom v rmen exchanger i s utrustad fall Inte kopplar v rmen exchanger till en vatten kran eller n got kylmedel k lla d r vattnet trycket r unregulated Inf r aldrig kylv tska eller n got organiska l sningsmedel in i n gon del av instrumentet Organiskt l sningsmedel ska orsaka irreparable skada till enheten Anv nd inte med buffert temperaturer ver det h gsta angivna tekniska specifikationerna verhettning skulle orsaka irreparabla skador p enheten e pvi English Ea French R HEN German R Ea Italian R E Spanish La Swedish R gum Waste Electrical and Electronic Equipment WEEE This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment Ce symbole ind
16. election The range of fragment sizes to be separated will determine the choice of agarose concentration for a gel Typical agarose concentration is 0 5 to 3 0 For large DNA fragments low percentage gels are required while for small DNA fragments high percentage gels are recommended Weak gels lt 0 5 agarose should be electrophoresed at low temperatures e g 4 C Agarose gels of 0 75 to 1 0 for routine electrophoresis are recommended for a wide range of separations 0 15 to 15 kb 2 4 agarose gels are usually selected for PCR fragment resolution If the gel has to be subsequently photographed thin gels 2 3 mm with low percentage agarose are better than thick or high percentage gels The latter produce increased opaqueness and autofluorescence e pl9 IMPORTANT Ensure that the agarose has cooled to between 50 and 60 C to prevent apparatus distortion e p20 Gel Pouring SUB6 SUB10 and SUB13 Units with removable gel casting trays and casting within the tank 1 Make sure that the silicone rubber gasket is in position e pushed evenly into the groove at the ends of the gel casting tray 2 Carefully place the casting tray sideways into the gel unit so that the gaskets form a seal with the sides of the running unit 3 Push the casting tray down onto the gel platform If the casting tray is too tight remove the silicone gaskets and replace it in the end groves stretching it a little 5 If the casti
17. forsyningskontroller af og afbryder kraftblyet for fjerning sikkerhedl get Cirkulerer bare vand eller 50 50 vand ethylene glykol gennem varmeveksleren i s fald udrustet Forbind ikke varmeveksleren til en vandhane eller nogen kolemiddelkilde hvor vandtrykket er unregulated Introducerer Aldrig antifreeze eller noget organisk oplesningsmiddel ind i nogen del af instrumentet Organiske opl sningsmidler vil for rsage uboelig skade til enheden Driver ikke med stedpudetemperaturer over maksimummet specificerede tekniske specifications Overheding vil for rsage uboelig skade til enheden e pii Belangrijke Informatie Dutch Indien deze uitrusting in een manier wordt gebruikt die niet door Hoefer Inc is gespecificeerd de bescherming die door de uitrusting is verzorgd kan worden geschaad Dit instrument is voor binnenlaboratoriumgebruik enkel ontworpen Enkel onderdelen en delen keurden goed of leverden door Hoefer Inc kan voor het bedienen worden gebruikt handhavend en onderhouden van dit product gebruik Enkel een netvoeding die CE is markeerde of veiligheid die door een is gecertificeerd die nationaal is herkend testene laboratorium Het veiligheidsdeksel moet in plaats voor het verbinden van de netvoeding leidt tot een netvoeding zijn Doe alle netvoedingscontroles Uit en koppel los de machtleiding voor het verwijderen van het veiligheidsdeksel Circuleer enkel water of 50 50 water ethy
18. g a microwave oven The agarose solution should be covered and the microwave set to low The agarose dissolves better if the microwave is periodically stopped and the solution swirled Before pouring the agarose solution should be checked for undissolved agarose crystals which can affect the mobility characteristics of gels If these are present agarose dissolving should be continued The agarose solution should be cooled to 50 60 C before pouring If poured at too hot a temperature the gel will be more likely to leak and also the comb may become distorted Agarose Gel Electrophoresis DNA Mobility DNA fragments as small as 1 kb or less can be separated using agarose gel electrophoresis For fragments smaller than 0 1 kb polyacrylamide gels are more suited RNA Mobility Either before or during electrophoresis RNA should be denatured For example e RNA fragments which have denatured with glyoxal and dimethyl sulphoxide can be separated on neutral agarose gels or e RNA can be fractionated on agarose gels containing methylmercuric hydroxide or formaldehyde RNA samples usually require longer runs or buffers that are easily depleted so it is necessary to circulate the buffer Northern analyses should not normally be run on a mini gel tank Separation Performance Gel concentration running buffer voltage temperature conformation and the presence of ethidium bromide all affect separation results Gel Concentration S
19. hickness 1 1 50r2mm Comb throughput 16 to 40 samples Comb slots 4 Migration distance between comb slots 5 cm Recommended running voltage 150 to 175V SUB20 Combs Max Sample Volume Thickness Sample Tooth Width Spacing in a 5 mm Catalog No mm Throughput mm mm deep well pl SUB20 C1 16 1 16 8 5 3 35 SUB20 C1 20MC 1 20 7 2 30 SUB20 C1 28 1 28 2 20 SUB20 C1 40MC 1 40 2 13 SUB20 C1 5 16 15 16 8 5 3 55 SUB20 C1 5 20MC 1 5 20 7 2 45 SUB20 C1 5 28 1 5 28 2 30 SUB20 C1 5 40MC 1 5 40 2 19 SUB20 C2 16 2 16 8 5 3 75 SUB20 C2 20MC 2 20 7 2 60 SUB20 C2 28 2 28 2 40 SUB20 C2 40MC 2 40 2 25 Multi channel pipette compatible ie n 1 e pl3 m SUB25 Combs SUB25 Maxi Plus Standard Horizontal The SUB25 Maxi Plus Standard Horizontal gel electrophoresis unit is ideal for high throughput analysis of nucleic acids Technical Specifications Unit dimensions W x L x H Gel size W x L Buffer volume Buffer re circulation ports Maximum sample capacity Combs Comb thickness Comb throughput Comb slots Migration distance between comb slots Recommended running voltage 33 x 56 x 9cm 25x 30cm 3000 ml 2 624 6 1 1 50r2mm 26 to 52 samples 12 2cm 150 to 200V Max Sample Volume Thickness Sample Tooth Width Spacing in a 5 mm Catalog No mm Throughput mm mm deep well ul SUB25 C1 26MC I 26 7 2 30 SUB25 C1 52MC 1 52 3 1 5 13 SUB25 C1 5 26MC 1 5 26 7 2 45 SUB25 C1 5 52MC 1 5 52 3 1 5 20 SU
20. ic is NOT resistant to aromatic or halogenated hydrocarbons ketones esters alcohol s over 25 and acids over 25 they will cause crazing especially of the UV transparent plastic and should NOT be used for cleaning DO NOT use abrasive creams or scourers Dry components with clean laboratory tissues prior to use General Care and Maintenance e To remove the safety lid push thumbs down on the plastic lugs and lift the lid vertically with your fingers Before use clean and dry the apparatus with DISTILLED WATER ONLY Before use and then on a monthly basis check the unit for any leaks at the bonded joints Place the unit on a sheet of dry tissue and then fill with DISTILLED WATER ONLY to the maximum fill line Any leakage will be seen on the tissue paper If any leakage is seen DO NOT ATTEMPT TO REPAIR OR USE THE APPARATUS but notify Hoefer immediately The replacement platinum electrodes are partially shrouded for protection However when cleaning the main tank DO NOT use cleaning brushes in the electrode area Usually a thorough rinse with distilled water is all that is required Ensure that the connectors are clean and dry before usage or storage Storage of Water Cooled Units We recommend complete drainage of cooling plate coil units before storage Store in a dark cupboard or cold room Using the Cooling Platform The Cooling Platform will already contain a small quantity of water from control te
21. ickness Sample Tooth Width Spacing in a 5 mm Catalog No mm Throughput mm mm deep well ul SUB15 C1 10 1 10 12 8 2 64 SUB15 C1 16MC 1 16 6 4 2 32 SUB15 C1 20 1 20 5 9 2 29 SUB15 C1 25 1 25 3 5 2 17 SUB15 C1 30MC 1 30 2 5 2 12 SUB15 C1 5 10 1 5 10 12 8 2 96 SUB15 C1 5 16MC 1 5 16 6 4 2 48 SUB15 C1 5 20 15 20 5 9 2 43 SUB15 C1 5 25 15 25 3 5 2 25 SUB15 C1 5 30MC 15 30 2 5 2 18 SUB15 C2 10 2 10 12 8 2 128 SUB15 C2 16MC 2 16 6 4 2 64 SUB15 C2 20 2 20 5 9 2 58 SUB15 C2 25 2 25 3 5 2 34 SUB15 C2 30MC 2 30 2 5 2 24 Multi channel pipette compatible e p17 e p18 Using The Horizontal Gel Electrophoresis Units Preparation of Agarose Gels The amount of agarose needed to give a particular gel needs to be calculated for example a 0 8 gel can be made by dissolving 0 8 grams of agarose powder in 100 mls of running buffer The type of running buffer used should always be the same for the gel as that used in the buffer tank The agarose needs to be fully dissolved before the gel can properly form This can be achieved by heating with swirling in a water bath or incubator set to 70 C or by heating on a magnetic heating block with a magnetic stirring bar inserted The flask should always be covered to prevent evaporation of the buffer and a higher concentration gel resulting These methods may take longer than 1 hour for the agarose to fully dissolve Alternatively the agarose can be dissolved in about 5 10 minutes usin
22. ique que les d chets relatifs l quipement lectrique et lectronique ne doivent pas tre jet s comme les ordures m nag res non tri es et doivent tre collect s s par ment Contactez un repr sentant agr du fabricant pour obtenir des informations sur la mise au rebut de votre quipement Dieses Symbol kennzeichnet elektrische und elektronische Ger te die nicht mit dem gew hnlichen unsortierten Hausm ll entsorgt werden d rfen sondern separat behandelt werden m ssen Bitte nehmen Sie Kontakt mit einem autorisierten Beauftragten des Herstellers auf um Informationen hinsichtlich der Entsorgung Ihres Ger tes zu erhalten Questo simbolo indica che i rifiuti derivanti da apparecchiature elettriche ed elettroniche non devono essere smaltiti come rifiuti municipali indifferenziati e devono invece essere raccolti separatamente Per informazioni relative alle modalit di smantellamento delle apparecchiature fuori uso contattare un rappresentante autorizzato del fabbricante Este s mbolo indica que el equipo el ctrico y electr nico no debe tirarse con los desechos dom sticos y debe tratarse por separado Contacte con el representante local del fabricante para obtener m s informaci n sobre la forma de desechar el equipo Denna symbol anger att elektriska och elektroniska utrustningar inte f r avyttras som osorterat hush llsavfall och m ste samlas in separat Var god kontakta en auktoriserad tillverkarrepresenta
23. leenglycol door de hitte exchanger zo ja uitrust Verbind de hitte exchanger naar een waterkraan of koelmiddelbron niet waar de waterdruk niet geregulariseerd is Stel Nooit antivriesmiddel of organische oplosmiddelen in deel van het instrument voor Organische oplosmiddelen zullen onherstelbare schade aan de eenheid veroorzaken Bedien niet met buffertemperaturen boven het maximum specificeerde technische specificaties Oververhittend zal onherstelbare schade aan de eenheid veroorzaken T rke Tietoa Finnish Jos tata varusteita k ytet n tavassa ei m ritetty Hoefer Inc suojelu ehk isty varusteille saattaa olla avuton Tama valine suunnitellaan sis laboratoriok yt lle vain Vain lis varusteet ja osat hyv ksyiv t tai toimitti Hoefer Inc oheen voi k ytt k ytt miselle valvoalle ja servicing t m tuote Vain k ytt k ytt j nnitett joka on CE merkitsi tai turvallisuus joka on todistanut aidoksi ohi joka on kansallisesti tunnustettnut testaaminen laboratoriota Turvallisuuskansi t ytyy olla paikallaan k yttoj nnitteeseen Kiert kaikki kaytt jannitevalvonnat ja irrottaa valtalyijyt ennen poistaminen turvallisuuskantta Kiert vain vesi tai 50 50 vesi ethylene glycol siin tapauksessa varustetun limm nvaihtimen l pi l yhdist l mm nvaihdinta vesinapautukseen eik j hdytysnestel hteeseen miss vesipaine on unregulated Pakkasne
24. lution Technical Specifications Unit dimensions W x L x H Gel size W x L Buffer volume Buffer re circulation ports Maximum sample capacity Combs Comb thickness Comb throughput Comb slots Cooling ports Migration distance between comb slots Recommended running voltage 33 x 56 x 9cm 25 x 30cm 3000 ml 2 624 6 1 1 50r2mm 26 to 52 samples 12 2 2 cm 150 to 250 V Max Sample Volume Thickness Sample Tooth Width Spacing in a 5 mm Catalog No mm Throughput mm mm deep well ul SUB25 C1 26MC 1 26 7 2 30 SUB25 C1 52MC 1 52 3 1 5 13 SUB25 C1 5 26MC 1 5 26 7 2 45 SUB25 C1 5 52MC 1 5 52 3 1 5 20 SUB25 C2 26MC 2 26 7 2 60 SUB25 C2 52MC 2 52 3 1 5 25 Multi channel pipette compatible e pl6 SUBHT Compatible Combs SUBHT High Throughput Horizontal The SUBHT High Throughput Horizontal gel electrophoresis unit is ideal for high throughput screening applications following PCR like Single Strand Polymorphism SSP analysis Technical Specifications Unit dimensions W x L x H 21 5 x 33 5 x 7 cm Gel size W x L 15x 15cm Buffer volume 1200 ml Buffer re circulation ports 2 Maximum sample capacity 120 Combs 4 Comb thickness 1 1 50r2mm Comb throughput 1 to 30 samples Comb slots 4 Migration distance between comb slots 3 5 cm Recommended running voltage 100 to 125V Max Sample Volume Th
25. n a UV transilluminator After each use rinse the apparatus with DISTILLED WATER ONLY After each use wash all components with a suitable water soluble disinfectant to reduce the risk of infection Ensure that the connectors are clean and dry before usage or storage Cleaning and Storage After use thoroughly rinse all components in deionized water and gently dry the gold electrode connectors with a soft tissue NEVER USE ORGANIC SOLVENTS Packing Lists SUB6 No Items Description Catalog No 1 Running tank lid L Gel casting tray SUB6 UT 2 Combs 1 0 mm 8 sample SUB6 C1 8 6 Colored loading strips SUB6 CS SUB10 1 Running tank lid T Gel casting tray SUB10 UT 2 Combs 1 0 mm 16 sample SUB10 C1 16 6 Colored loading strips SUB10 CS SUB13 I Running tank lid 1 Gel casting tray SUB13 UT 2 Combs 1 0 mm 16 sample SUB13 C1 16 2 Buffer recirculation ports SUB BRP 6 Colored loading strips SUB13 CS SUB15 1 Running tank lid 1 Gel casting tray SUB15 UT 2 Casting gates silicone seals SUB15 CG 2 Combs 1 0 mm 16 sample SUB15 C1 16 2 Buffer recirculation Ports SUB BRP 6 Colored loading strips SUB15 CS SUB20 1 Running tank lid 1 Gel casting tray SUB20 UT 2 Casting gates silicone seals SUB20 CG 2 Combs 1 0 mm 16 sample SUB20 C1 16 2 Buffer recirculation ports SUB BRP 6 Colored loading strips SUB20 CS SUB25 No Items Description Catalog No 1 Running tank lid
26. ng tray is too loose remove the silicone gaskets and replace them in the end grooves compressing it a little 6 Place the gel unit on a level surface 7 Position the required comb s into the slot s in the gel casting tray 8 Prepare 22 5 60 or 100 mls of the desired agarose for units SUB6 SUB10 or SUB13 respectively This will give a gel depth of 5 mm For a 10 mm deep gel prepare 45 125 or 200 mls respectively 9 Using a 1 ml disposable pipette seal the silicone seal with some of the cooled agarose 10 Pour in the agarose smoothly so as not to generate bubbles If bubbles do occur these can be smoothed to the side of the gel and dispersed using a clean gloved hand 11 Allow the agarose to set ensuring that the gel remains undisturbed 12 Carefully remove the gel casting tray from the running unit Either remove the end silicone rubber gaskets or leave them in place 13 Place the gel casting tray in the running position such that the wells are nearest to the black electrode cathode 14 Fill the tank with running buffer so that the buffer just submerges the gel 15 Carefully remove the comb IMPORTANT Ensure that the agarose has cooled to between 50 and 60 C to prevent apparatus distortion SUB15 SUB20 SUB20C SUB25 SUB25C and SUBHT Units with removable gel casting trays and gel casting gates 1 Place the casting gates into the slots provided in the gel casting tray with the end silicone rubber g
27. ngsmittel in jeden Teil des Instrumentes ein Organische L sungsmittel werden nicht wiedergutzumachenden Schaden der Einheit verursachen Mit Puffertemperaturen ber angegebenen technischen Spezifizierungen des Maximums nicht funktionieren Die berhitzung wird nicht wiedergutzumachenden Schaden der Einheit verursachen Informazioni Importanti Italian Se quest apparecchiatura usata in un modo specificato da Hoefer Inc la protezione fornito dall apparecchiatura potrebbe essere indebolita Questo strumento disegnato per l uso di laboratorio interno solo Solo gli accessori e le parti hanno approvato o hanno fornito da Hoefer Inc potrebbe essere usato per operare per mantenere e per revisionare questo prodotto usa Solo un alimentatore che CE ha marcato o la sicurezza certificato da un nazionalmente riconosciuto testando il laboratorio coperchio di sicurezza deve essere nel luogo prima di collegare i piombi di alimentatore a un alimentatore Spegne tutto i controlli di alimentatore e disinserisce i piombi di potere prima di togliere il coperchio di sicurezza Circola solo l acqua o 50 50 glicole di acqua etilene attraverso lo scambiatore di calore se cosi equipaggiato Non collegare lo scambiatore di calore a un rubinetto di acqua o qualunque fonte di refrigerante dove la pressione di acqua sregolata Non introduce mai l antigelo o qualunque solvente organico in qualunque parte
28. nt f r information ang ende avyttring av utrustningen e pvii CE Specifications Application For laboratory use only in the electrophoretic separation of proteins and nucleic acids Country of Origin United States Manufacturer Hoefer Inc 84 October Hill Road Holliston MA 01746 Toll Free 1 800 227 4750 Phone 1 508 893 8999 Fax 1 508 429 5732 E mail salesQhoeferinc com Construction e Rugged acrylic construction e All acrylic joints chemically bonded e Doubly insulated cables rated safe up to 1 000 V e Gold plated electrical connectors corrosion free and rated safe up to 1 000 V e Recessed power connectors integral with the safety lid 0 2 mm diameter platinum electrodes 99 99 pure e User replaceable platinum electrodes e User friendly clamping system e Wide range of accessories Environmental Conditions e This apparatus is intended for indoor use only e his apparatus can be operated safely up to an altitude of 2 000 m e The normal operating temperature range is between 4 C and 65 C e Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C The apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664 POLLUTION DEGREE 2 states that Normally only non conductive pollution occurs Occasionally however a temporary conductivity caused by condensation must be expected Safety Precautions e REA
29. ser da ada Este instrumento es dise ado para el uso interior del laboratorio s lo S lo accesorios y partes aprobaron o suministraron por Hoefer Inc puede ser utilizado para operar para mantener y para atender a este producto S lo utiliza una alimentaci n que es CE marc o la seguridad certificada por un nacionalmente reconocido probando el laboratorio Latapa de la seguridad debe estar en el lugar antes de conectar la alimentaci n lleva a una alimentaci n Apaga todos controles de alimentaci n y desconecta los plomos del poder antes de quitar la tapa de la seguridad Circula s lo agua o 50 50 glicol de agua etileno por el intercambiador de calor si se es el caso equiparon No conecte el intercambiador de calor a un toque de la agua ni cualquier fuente del l quido refrigerante donde la presi n del agua est libre Nunca introduce anticongelante ni alg n solvente org nico en cualquier parte del instrumento Los solventes org nicos causar n da o irreparable a la unidad No opera con temperaturas de b fer encima del m ximo especific especificaciones t cnicas Recalentar causar da o irreparable a la unidad Viktig Information Swedish om denna utrustning anv nds i ett s tt som inte har specificeras av Hoefer Inc skyddet tillhandah ll vid utrustningen kan skadas Detta instrument formges f r inomhuslaboratorium anv ndning bara Bara medhj lpare och delar godk nd
30. ssories and parts approved or supplied by Hoefer Inc may be used for operating maintaining and servicing this product Only use a power supply that is CE marked or safety certified by a nationally recognized testing laboratory The safety lid must be in place before connecting the power supply leads to a power supply Turn all power supply controls off and disconnect the power leads before removing the safety lid Circulate only water or 50 50 water ethylene glycol through the heat exchanger if so equipped Do not connect the heat exchanger to a water tap or any coolant source where the water pressure is unregulated Never introduce antifreeze or any organic solvent into any part of the instrument Organic solvents will cause irreparable damage to the unit Do not operate with buffer temperatures above the maximum specified technical specifications Overheating will cause irreparable damage to the unit Dule it Informace Czech Pokud by toto za zen je pou ito zp sobem kter nen podle Hoefer Inc ochrana poskytovan na z klad za zen m e b t naru ena Tento n stroj je ur en pro vnit n pou it v laborato i pouze Pouze p slu enstv a sti schv len nebo poskytnut ch Hoefer Inc mohou b t pou ity pro provoz dr bu a dr b tohoto v robku zdroj nap jen pou vaj jen e je opat en ozna en m CE osv d ena nebo bezpe nost vnitrost tn uznan mi zku e
31. ste eik orgaaninen liuotin v lineen osassa ei esitele Koskaan Orgaaniset liuottimet aiheuttavat korvaamattoman vahingon yksikk n Ei k yt puskuria yll olevia l mp tiloja enint n m ritetyill teknisill t smennyksill Ylikuumeneminen aiheuttaa korvaamattoman vahingon yksikk n Information Importante French Si cet quipement est utilis dans une mani re pas sp cifi par Hoefer Inc la protection fourni par l quipement pourrait tre diminu e Cet instrument est con u pour l usage de laboratoire int rieur seulement Seulement les accessoires et les parties ont approuv ou ont fourni par Hoefer Inc pourrait tre utilis pour fonctionner maintenir et entretenir ce produit utilise Seulement une alimentation qui est CET a marqu ou la s curit certifi par un nationalement reconnu essayant le laboratoire Le couvercle de s curit doit tre sa place avant connecter l alimentation mene une alimentation Tourner tous contr les d alimentation de et d brancher les avances de pouvoir avant enlever le couvercle de s curit Circuler seulement de l eau ou 50 50 glycol d eau thyl ne par l exchanger de chaleur si si quip Ne pas connecter l exchanger de chaleur un robinet d eau ou la source d agent de refroidissement o la pression d eau est non r gul e Ne Jamais introduire d antigel ou du dissolvant organique dans n importe quelle partie de
32. sts The Cooling Platform can be used in two ways Static water can be used as a simple heat sink or the tank can be actively regulated using flowing water from a tap or water bath flow cooler 1 Static Temperature Regulation Attach a short length of rubber hose to each connector Incline the unit at an angle of approximately 45 degrees with the ports uppermost Use a funnel to fill the Cooling Platform with deionized water containing 0 02 w v sodium azide preservative to prevent bacterial and algal growth When filled keep the unit inclined and attach clamps to each piece of rubber hose The unit can be cooled before an electrophoresis run if required DO NOT FREEZE Active Temperature Regulation 1 Attach a short length of rubber hose to each connector Attach one end of the rubber hose to the outlet port of a circulating water bath flow cooler and the other end to the inlet port Alternatively attach one end of a rubber hose to a water supply and allow the other rubber hose drain to waste The maximum recommended water flow rate is 1 Liter min DO NOT exceed this figure If you are using a circulating water bath flow cooler which exceeds this flow rate you can attach a T connector in line One branch of the connector can return water to the bath and the other can flow to the Cooling Platform and incorporate a flow regulator such as an adjustable tubing clamp Measure and adjust the flow rate before at
33. taching the line to the gel unit Gel Loading Instructions for the SUBHT 1 Use a 12 channel multi pipette to aspirate sample from row A of a 96 well plate Wells A1 A12 Place the left most tip containing sample A1 into the corresponding gel well labeled A1 Ath well in from the left side and dispense Samples from wells A1 though A12 will be dispensed in every other well of the gel Aspirate samples from row B wells B1 B12 and place left most tip containing sample B1 into the corresponding gel well labeled B1 5th well in from the left side to the right of well A1 Repeat steps 1 3 for rows C and D E and F and G and H Use a single channel pipette to load up to 3 size marker samples on either side of each row 456 7 8 9 10 1 12 A 000000000000 OO000OOOO0C0CO Mi M2 M3 Ai BI A2 B2 AS BS At B4 AS B5 AG B6 AT B7 AB BB A9 89 A OBIOAT BI AIZB12 NM M5 ME IMPORTANT Acrylic plastic is NOT resistant to aromatic or halogenated hydrocarbons ketones esters alcohol s over 25 and acids over 25 they will cause crazing especially of the UV transparent plastic and should NOT be used for cleaning DO NOT use abrasive creams or scourers Dry components with clean tissues prior to use At the End of the Run 1 Turn the power supply settings to zero turn off mains supply and disconnect the power leads Visualize the run progression or final separation o
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