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Agilent 1260 Infinity Binary Pump VL

1. Figure 4 Rear View of Binary Pump Electrical Connections and Label 1260 Binary Pump VL User Manual 17 1 introduction Interfaces Table2 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD optional LAN on board RS 232 Analog APG Remote The Agilent 1200 Infinity Series modules provide the following interfaces Special Pumps G1310B Iso Pump G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G4220A B Bin Pump G1361A Prep Pump Samplers G1329B ALS G2260A Prep ALS G1364B FC PS G1364C FC AS G1364D FC uS G1367E HiP ALS G1377A HiP micro ALS G2258A DL ALS G4226A ALS Detectors G1314B VWD VL G1314C VWD VL G1314E F VWD Yes No Yes Yes Yes Yes Yes No No Yes No No No No No Yes Yes Yes Yes Yes Yes Yes Yes Yes No No No No No Yes Yes Yes Yes Yes Yes Yes Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330B THERMOSTAT for G1330B CAN DC OUT for CAN slaves 18 1260 Binary Pump VL User Manual Introduction 1 Table 2 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G4212A B DAD 2 No Yes Yes 1 Yes G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 1 Yes G1362A RID
2. Seal keeper E CE Seal wash a support ring a J cre S74 gt Plunger housing Plunger E 2 Using the blade of a flat head screwdriver remove seal wash gasket and the secondary seal from the support ring N S Secondary T seal F a lt Support ring lt _ lt oe 1260 Binary Pump VL User Manual 147 Maintenance Exchanging the Wash Seals 3 Using the insert tool press the seal spring pointing upwards into the recess of the support ring Place a seal wash gasket in the recess of the support ring and replace the seal keeper gt Insert tool LY Seal L Support ring N 4 Reassemble the pump head assembly see Reinstalling the Pump Head Assembly on page 149 148 1260 Binary Pump VL User Manual Maintenance 9 Reinstalling the Pump Head Assembly When When reassembling the pump Tools required 3 mm hexagonal key 4 mm hexagonal key Parts required p n Description 1 79846 65501 Pump head grease 1 Slide the pump head assembly onto the pump drive Purge valve holder Pump head screws l 2 Using a 4 mm hexagonal key tighten the pump head screws stepwise with increasing torque 3 Using a3 mm hexagonal key fix the purge valve holder to the pump head 1260 Binary Pump VL User Manual 149 9 Maintenance Reinstalling the Pump Head Assembly 4 Reconnect the tubing and capillaries to the connector 150 1260 Binary Pump VL Use
3. E57 1260 Binary Pump VL User Manual Agilent Module to BNC Connector Identifying Cables 11 p n 8120 1840 Pin BNC Pin Agilent Signal Name module nn dl Shield Shield Analog lt _ EO Center Center Analog Agilent Module to General Purpose p n 01046 60105 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Black Analog 3 Red Analog E xg 1260 Binary Pump VL User Manual 195 11 Identifying Cables Remote Cables 196 O O JNE One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3394 Pin Agilent Signal Name Active module TTL Z 9 1 White Digital ground a NC 2 Brown Prepare run Low 3 3 Gray Start Low z NC 4 Blue Shut down Low 5 NC 5 Pink Not connected i NC 6 Yellow Power on High o 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators p n 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1260 Binary Pump VL User Manual Identifying Cables 11 Agilent Mo
4. 1260 Binary Pump VL User Manual Suggested actions Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly Exchange the CAN cable Switch off the system Restart the system and determine which module or modules are not recognized by the system 89 7 90 Error Information Leak Sensor Short The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause 1 Defective flow sensor 2 Leak sensor incorrectly routed being pinched by a metal component Leak Sensor Open Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause 1 Leak sensor not connected to the main board 2 Defective leak sensor 3 Leak sensor incorrectly routed being pinched by a metal component Suggested actio
5. Agilent Technologies Notices Agilent Technologies Inc 2008 2010 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61312 90007 Edition 06 10 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate writte
6. Identifying Cables 11 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connec ted to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 Violet 2 BCD7 80 4 Blue 3 BCD 6 40 m Yellow 4 BCD 4 10 SS Black 5 BCD 0 1 ay Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low 1260 Binary Pump VL User Manual 199 11 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module 1 1 BCD 5 20 2 2 BCD7 80 8e 7 3 3 BCD6 40 4 4 BCD 4 10 5 5 BCDO 1 1e 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 200 1260 Binary Pump VL User Manual CAN LAN Cables Identifying Cables 11 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 1260 Binary Pump VL User Manual Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to
7. Error Information 7 Open Cover The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during operation Reinstall the top foam 2 Foam not activating the sensor Please contact your Agilent service representative 3 Dirty or defective sensor Please contact your Agilent service representative 4 Rear of the module is exposed to strong Ensure that the rear of module is not directly direct sunlight exposed to strong sunlight 1260 Binary Pump VL User Manual 93 7 Error Information Module Error Messages 94 Restart Without Cover Restart Without Cover The module was restarted with the top cover and foam open The sensor on the main board detects when the top foam is in place If the module is restarted with the foam removed the module switches off within 30 s and the error message is generated Probable cause Suggested actions 1 The module started with the top cover and Please contact your Agilent service foam removed representative 2 Rear of the module is exposed to strong Ensure that the rear of module is not directly direct sunlight exposed to strong sunlight Zero Solvent Counter Solvent Zero Counter Pump firmware version A 02 32 and higher allow to set solvent bottle fillings in the data system If the volume l
8. Pump channel A did not reach the delivery phase Pump channel B did not reach the delivery phase Pump channel A did not reach the take in phase Pump channel B did not reach the take in phase Solvent volume not delivered within the specified time Probable cause Suggested actions 1 Purge valve open Ensure that purge valve is closed 2 Leak at fittings purge valve active inlet Ensure pump components are seated valve outlet valve or piston seals correctly If there are still signs of a leak exchange the appropriate seal purge valve active inlet valve outlet valve piston seal Exchange defective capillaries 3 Flow changed after starting test Ensure correct operating condition for the special application in use 4 Defective pump drive assembly Please contact your Agilent service representative 1260 Binary Pump VL User Manual 113 7 Error Information Electronic fuse of SSV The electronic fuse protecting the solvent selection valve electronics has blown Probable cause Suggested actions 1 Recoverable error of the SSV electronic Restart module the electronic fuse can recover If not contact Agilent service 2 Short cut of SSV cable Replace cable between board and SSV 114 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 8 Test Functions and Calibration Pressure Test 116 Description 116 Positioning the Blank Nut 117 Running the Pressure Test 118 Evaluating the Results
9. Ramp 5 Piston A1 delivers 50 uL min for approximately 8 s Plateau 3 Piston A1 delivers with a flow rate of 3 uL min for 30 s Ramp 6 Piston B1 delivers 50 uL min for approximately 7 s Plateau 4 Piston B1 delivers with a flow rate of 3 uL min for approximately 30 s At the end of the fourth plateau the test is finished and the pump switches off 1260 Binary Pump VL User Manual Test Functions and Calibration 8 Running the Leak Test When Tools required Parts required Preparations If problems with the pump are suspected Wrench 1 4 inch p n Description 1 G1313 87305 Restriction Capillary 1 01080 83202 Blank nut 1 500 ml lsopropanol Place two bottles of LC grade isopropyl alcohol in channels A and B If a solvent selection valve is installed place the LC grade isopropanol in channels A2 and B2 Make absolutely sure that all parts of the flow path that are part of the test are very thoroughly flushed with isopropanol before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail Running the test from the Agilent Lab Advisor 1 Select the Leak Test from the Test Selection menu 2 Start the test and follow the instructions Make sure to release the pressure by slowly opening the purge valve when the test has finished Evaluating the Results on page 124 describes the evaluation and interpretation of t
10. Servo Restart Failed Servo Restart Failed Pump channel A B Servo Restart Failed Pump channel B The pump motor in the module was unable to move into the correct position for restarting When the module is switched on the first step is to switch on the C phase of the variable reluctance motor The rotor should move to one of the C positions The C position is required for the servo to be able to take control of the phase sequencing with the commutator If the rotor is unable to move or if the C position cannot be reached the error message is generated Probable cause Suggested actions 1 Disconnected or defective cable Please contact your Agilent service representative 2 Mechanical blockage of the module Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly 3 Defective pump drive assembly Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative 106 1260 Binary Pump VL User Manual Error Information 7 Pump Head Missing Pump Head Missing Pump channel A B Pump Head Missing Pump channel B The pump head end stop in the pump was not found When the pump restarts the metering drive moves forward to the mechanical end stop Normally the end stop is reached within 20 seconds indicated by an increase in motor current If the end point is not found within 20 seconds the error messag
11. Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren 210 Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1260 Binary Pump VL User Manual Appendix 12 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 1260 Binary Pump VL User Manual 211 12 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 1
12. 3 Using a 4 mm hexagonal key stepwise loosen and remove the two pump head screws Pump head screws 4 Using a 3 mm hexagonal key loosen the purge valve holder and remove it from pump head body 1260 Binary Pump VL User Manual 141 9 Maintenance embling the pump head Disassembling the pump head Place the pump head with the front face onto a bench Open the two hex screws on the rear side with a 3 mm hex driver Open the PEEK lock screw two to three turns Aa QO N Pull the spring housing up and remove it from the pump head Spring housing lt 5 Giese screw a A 142 1260 Binary Pump VL User Manual Maintenance 9 Exchanging the Pump Seals When Seals leaking if indicated by the results of the pump test check both pump heads individually Tools required Wrench 1 4 inch 3 mm hexagonal key 4 mm hexagonal key Insert tool Parts required p n Description 1 0905 1503 Piston seal PTFE carbon filled black pack of 2 default 1 0905 1420 PE seals pack of 2 1 5022 2159 Restriction capillary Preparations Switch off the pump at the main power switch Remove the front cover to have access to the pump mechanics 1 Disassemble the pump head assembly see Disassembling the pump head on page 142 2 Use the insert tool to carefully remove the seal from the pump head Se Insert tool A Pump seal Pump head 1260 Binary Pump VL User Manual 143 9 Maintenance Exchangi
13. Bottle Head Assembly 185 Hydraulic Path with Solvent Selection Valve 186 Hydraulic Path without Solvent Selection Valve 188 This chapter provides information on parts for maintenance ee Agilent Technologies 171 10 Parts for Maintenance Parts and Materials Parts and Materials Figure 28 Overview of Main Assemblies Front View Table 20 Repair Parts Pump Housing and Main Assemblies Front View Item Description Part Number 1 Pump head p n G1311 60004 2 Pump drive assembly p n G1311 60001 Exchange assembly pump drive p n G1311 69001 172 1260 Binary Pump VL User Manual Parts for Maintenance Table 20 Repair Parts Pump Housing and Main Assemblies Front View Item Description Part Number 10 3 4 Cable assembly AIV to main board High pressure pump main board HPM Exchange assembly HPM board Cable assembly solvent selection valve Fan assembly Damping unit Solvent selection valve half of a complete valve Screw solvent selection valve Leak pan pump p n G1311 61601 p n G1312 66540 p n G1312 69540 p n G1312 61602 p n 3160 1017 p n 79835 60005 p n G1312 60000 p n 5022 2112 p n 5042 8590 6 7 X Agden Techestogios Ne 194 rdeci tie 013128 LALLU Sewin oenmatio BORNE Figure 29 Overview of Main Assemblies Rear View 1260 Binary Pump VL User Manual 173 10 Parts for Maintenance 174 Table 21 I
14. G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1316A C TCC 2 No No Yes No Yes G1322A DEG No No No No No Yes AUX G1379B DEG No No No Yes No No AUX G4227A Flex Cube 2 No No No No No G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN e CAN connectors as interface to other modules e LAN connector as interface to the control software RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output 1260 Binary Pump VL User Manual 19 1 20 Introduction Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369A B LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a connected PC with the appropriate control software If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed i
15. e q es s L Parts for Maintenance 10 Hydraulic Path without Solvent Selection Valve Figure 37 Hydraulic Path without Solvent Selection Valve 1260 Binary Pump VL User Manual 189 10 Parts for Maintenance Hydraulic Path without Solvent Selection Valve 190 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 11 Identifying Cables Cable Overview 192 Analog Cables 194 Remote Cables 196 BCD Cables 199 CAN LAN Cables 201 External Contact Cable 202 Agilent Module to PC 203 Agilent 1200 Module to Printer 204 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules ot Agilent Technologies 191 11 Identifying Cables Cable Overview 192 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n Description 35900 60750 Agilent module to 3394 6 integrators 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs Remote cables p n Description 03394 60600 Agilent module to 3396A Series integrators 3396 Series Il 3395A integrator see details in section Remote Cables on page 196 03396 61010 Agilent module to 3396 Series III 3395B integrators 5061 3378 Agilent module to Agilent 35900 A D converters or HP 1050 1046A 1049A 01046 60201 Agilent module to general purpose BCD cables
16. p n Description 03396 60560 Agilent module to 3396 integrators G1351 81600 Agilent module to general purpose 1260 Binary Pump VL User Manual Identifying Cables 11 CAN caables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n Description G1103 61611 External contact cable Agilent module interface board to general purposes RS 232 cables p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m 1260 Binary Pump VL User Manual 193 11 Identifying Cables Analog Cables 194 One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog
17. 119 System Pressure Test failed 120 Leak Test 121 Description 121 Running the Leak Test 123 Evaluating the Results 124 This chapter describes the tests for the module gy Agilent Technologies 115 8 Test Functions and Calibration Pressure Test Description The pressure test is a quick built in test designed to demonstrate the pressure tightness of the system The test involves monitoring the pressure profile while the binary pump runs through a predefined pumping sequence The resulting pressure profile provides information about the pressure tightness of the system Step 1 The test begins with the initialization of both pumpheads After initialization pistons Al and B1 are both at the top of their stroke Next pump A begins pumping solvent with a flow rate of 510 uL min and stroke of 100 uL The binary pump continues to pump until a system pressure of 390 bar bar is reached For this test channel A is active which is directly connected to chamber 2 in channel B see Figure 2 on page 10 To test the pressure tightness of the pump use the leak test see Description on page 121 Step 2 When the system pressure reaches 390 bar bar the binary pump switches off The pressure drop from this point onwards should be no more than 2 bar min 116 1260 Binary Pump VL User Manual Test Functions and Calibration 8 Positioning the Blank Nut To test the complete system s pressure tightness the blank nut should be
18. 170 The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if a new module with newer firmware is added to a system or if third part control software requires a special version LAN RS 232 Firmware Update Tool or Agilent Diagnostic Software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 To load the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 10 Parts for Maintenance Parts and Materials 172 Pump Head Assembly Without Seal Wash 175 Pump Head Assembly with Seal Wash Option 177 Outlet Valve 179 Purge Valve Assembly 180 Active Inlet Valve Assembly 181 Accessory Kit 182 Seal Wash Option G1312 68711 183 Solvent Cabinet 184
19. an error condition Refer to the Service Manual for the degasser or the 1260 pump that has the degasser built in 1260 Binary Pump VL User Manual Remote Timeout Error Information 7 A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition Synchronization Lost Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause 1 CAN cable disconnected 2 Defective CAN cable 3 Defective main board in another module
20. and at least one other plateau positive Probable cause 1 Leaking outlet valve in channel A 2 Loose pump head screws in channel A 3 Leaking seal or scratched piston in channel A2 Suggested actions Clean the outlet valve in channel A Ensure the sieve in the outlet valves are installed correctly Tighten the outlet valve Ensure the pump head screws in channel A are tight Exchange the pump seals in channel A Check the piston for scratches Exchange if scratched Second plateau negative or unstable and at least one other plateau positive Probable cause 1 Leaking outlet valve in channel B 2 Loose pump head screws in channel B 3 Leaking seal or scratched piston in channel B2 Suggested actions Clean the outlet valve in channel B Ensure the sieve in the outlet valves are installed correctly Tighten the outlet valve Ensure the pump head screws in channel B are tight Exchange the pump seals in channel B Check the piston for scratches Exchange if scratched 1260 Binary Pump VL User Manual Test Functions and Calibration 8 Third plateau negative or unstable and at least one other plateau positive Probable cause Suggested actions 1 Airin channel A or new seals not yet seated Flush channel A thoroughly with isopropanol under pressure use restriction capillary 2 Loose active inlet valve in channel A Tighten the active inlet valve in channel A 14 mm wrench Do not overtighten 3 Loose pu
21. from the active seal wash option kit into the plunger housing Figure 23 Inserting the active seal wash support rings 11 Place the support rings on the plunger housing plungers not installed and snap the pump head and plunger housing together 12 Insert the plungers and carefully press them into the seal 13 Tighten the lock screw 14 Slide the pump head assembly onto the metering drive Apply a small amount of pump head grease Pump head grease p n 79846 65501 to the pumphead screws and the balls of the spindle drive Tighten the pumphead screws stepwise with increasing torque 15 Reconnect all capillaries tubes and the active inlet valve cable to its connector 16 Route the wash inlet tube into a bottle filled with a mixture of distilled water and isopropanol 90 10 and place the bottle above the pump in the solvent cabinet 162 1260 Binary Pump VL User Manual Installing the Activ 17 Route the outlet of the wash tube into a waste container Figure 24 Pumphead after completed installation 1260 Binary Pump VL User Manual Maintenance al Wash Option 163 9 Maintenance Exchanging the Active Inlet Valve When Tools required Parts required Preparations 164 If internally leaking backflow Wrench 14 mm p n Description 1 G1312 60025 Active inlet valve body 1 5062 8562 Valve cartridge 400 bar Switch off binary pump at power switch 1 Remove the front cover 2 Unplug the active inl
22. gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 208 1260 Binary Pump VL User Manual Appendix 12 The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product s Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1260 Binary Pump VL User Manual 209 12 Appendix Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed
23. influencing the flow stability of the pump The amplitude of the pressure pulsation depends mainly on the stroke volume and the compressibility compensation for the solvent in use Small stroke volumes generate pressure pulsations of smaller amplitude than higher stroke volumes at the same flow rate In addition the frequency of the pressure pulsations is higher This decreases the influence of flow pulsations on quantitative results In gradient mode smaller stroke volumes result in a lower flow ripple improve composition ripple The module uses a processor controlled spindle system for driving its pistons The normal stroke volume is optimized for the selected flow rate Small flow rates use a small stroke volume while higher flow rates use a higher stroke volume By default the stroke volume for the pump is set to AUTO mode This means that the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended 1260 Binary Pump VL User Manual 13 1 Introduction Early Maintenance Feedback 14 Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when
24. pH lt 2 3 should not contain acids which attack stainless steel High pressure binary mixing Comments 1260 Binary Pump VL User Manual Site Requirements and Specifications 2 Table 14 Performance Specification Agilent 1260 Infinity Binary Pump VL G1312C Delay volume 180 480 uL without mixer 600 900 pL with mixer both dependent on back pressure Composition range settable range 0 100 recommended range 1 99 or 5 pL min per channel whatever is greater Composition precision lt 0 5 absolute Composition accuracy 0 5 abs at 1 mL min water caffeine tracer Control and data evaluation Agilent ChemStation for LC Analog output For pressure monitoring 2 mV bar one output Communications Controller area network CAN RS 232C APG Remote ready start stop and shut down signals LAN optional For use with flow rates below 500 pL min a vacuum degasser is required 1260 Binary Pump VL User Manual 37 2 Site Requirements and Specifications Performance Specifications 38 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 3 Installing the Pump Unpacking the Binary Pump 40 Damaged Packaging 40 Delivery Checklist 40 Optimizing the Stack Configuration 43 One Stack Configuration 43 Two Stack Configuration 46 Installing the Binary Pump 48 Connecting Modules and Control Software 51 Connecting Agilent 1260 Infinity Modules 51 Connecting an Agilent 1260 Infinity Vacu
25. remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation 1260 Binary Pump VL User Manual Appendix 12 Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fume
26. switches DOWN best settings Bootp mode for LAN e For specific LAN modes switches 3 8 must be set as required For boot test modes switches 1 2 must be UP plus required mode gt y SL Agilent Technaoges gt D A z 3 3 Figure 6 Location of Configuration Switch example shows a G4212A DAD To perform any LAN configuration SW1 and SW2 must be set to OFF For details on the LAN settings configuration refer to chapter LAN Configuration 24 1260 Binary Pump VL User Manual Introduction 1 Table5 8 bit Configuration Switch with on board LAN Mode Function SW 1 SW 2 SW 3 SW 4 SW 5 SW 6 SW7 SW 8 LAN 0 0 Link Configuration Init Mode Selection Auto negotiation 0 xX x x x x 10 MBit half duplex 1 0 0 X X xX 10 MBit full duplex 1 0 1 xX x xX 100 MBit half duplex 1 1 0 x X X 100 MBit full duplex 1 1 1 Xx x xX Bootp x xX x 0 0 0 Bootp amp Store Xx X X 0 0 1 Using Stored x X x 0 1 0 Using Default Xx xX xX 0 1 1 TEST 1 1 System NVRAM Boot Resident System 1 x Revert to Default Data Coldstart X X X 1 Legend 0 switch down 1 switch up x any position When selecting the mode TEST the LAN settings are Auto Negotiation amp Using Stored For explanation of Boot Resident System and Revert to Default Data Coldstart refer to Special Settings on page 29 1260 Binary Pump VL User Manual 25 1 Introduction Setting the 8 bi
27. the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1260 Binary Pump VL User Manual Introduction 1 Instrument Layout The industrial design of the module incorporates several innovative features
28. to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1260 Binary Pump VL User Manual Contents Contents 1 Introduction 7 Introduction to the Binary Pump 8 Overview of the Binary Pump 9 Early Maintenance Feedback 14 Instrument Layout 15 Electrical Connections 16 Interfaces 18 Setting the 8 bit Configuration Switch On Board LAN 24 2 Site Requirements and Specifications 31 Site Requirements 32 Physical Specifications 35 Performance Specifications 36 3 Installing the Pump 39 Unpacking the Binary Pump 40 Optimizing the Stack Configuration 43 Installing the Binary Pump 48 Connecting Modules and Control Software 51 Flow Connections of the Binary Pump with Solvent Selection Valve 54 Flow Connections of the Binary Pump Without Solvent Selection Valve 57 Priming and Purging the System 60 4 Using the Binary Pump 63 Hints for Successful Use of the Binary Pump 64 Solvent Information 66 Prevent Blocking of Solvent Filters 67 Algae Growth in HPLC Systems 68 1260 Binary Pump VL User Manual Contents Optimizing Perform
29. without on board LAN They are identified as LAN and no LAN Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 11 Boot Resident Settings without on board LAN Mode Select SW1 sw2 SW3 SW4 SW5 SW6 SW7 Swe LAN No LAN TEST BOOT 1 1 1 0 0 0 0 0 TEST BOOT 1 1 0 0 1 0 0 0 CAUTION Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed 1260 Binary Pump VL User Manual 29 1 Introduction Table 12 Forced Cold Start Settings without on board LAN Mode Select SW1 sw2 SW3 sw4 SW5 SW6 SW7 Swe LAN TEST BOOT 1 1 0 0 0 0 0 1 No LAN TEST BOOT 1 1 0 0 1 0 0 1 30 1260 Binary Pump VL User Manual 1260 Binary P
30. 04 7 Mixing capillary p n G1312 67302 8 Connection tube p n G1311 67304 9 Capillary pump to injector device p n G1312 67305 Capillary pump to thermostattable autosampler p n G1329 87300 10 Waste tube 5 m p n 5062 2461 Capillary damper to purge valve p n G1312 67301 1 Capillary needed when pump used without mixer installed 1260 Binary Pump VL User Manual Parts for Maintenance 10 Hydraulic Path with Solvent Selection Valve ISEN NWT LL a YAO 10 9 Figure 36 Hydraulic Path with Solvent Selection Valve 1260 Binary Pump VL User Manual 187 10 Parts for Maintenance Hydraulic Path without Solvent Selection Valve 188 Table 24 Hydraulic Path without Solvent Selection Valve Item Description Part Number 1 Capillary mixer to purge valve 2 Capillary outlet ball valve to piston 2 3 Mixer 4 Bottle head assembly 5 Capillary damper to mixer 6 Restriction capillary 7 Mixing capillary 8 Capillary pump to injection device Capillary pump to thermostattable autosampler 9 Waste tube 5 m 10 Adapter AIV to bottle head assembly 1 Capillary damper to purge valve p n 61312 67308 p n G1312 67300 p n 61312 87330 p n G1311 60003 p n 61312 67307 p n 61312 67304 p n G1312 67302 p n G1312 67305 p n G1329 87300 p n 5062 2461 p n 0100 1847 p n G1312 67301 i Capillary needed when pump used without mixer installed 1260 Binary Pump VL User Manual AE ne os AL i
31. 1 inlet valve fuse 101 inlet valve missing 105 insert tool 147 installation pump module 48 installation bench space 34 site requirements 31 instrument layout 15 1260 Binary Pump VL User Manual Index interface board 168 interface cable 50 interfaces 18 internet 214 introduction to the pump 8 L LAN cable 201 leak sensor open 90 leak test evaluation 124 leak test 121 leak sensor short 90 leak 92 line frequency 35 line voltage 35 liquimeter 144 lithium batteries 210 M main assemblies overview 132 maintenance feedback 14 replacing firmware 170 170 message ignition without cover 93 93 remote timeout 89 minimum flow rate 64 missing pressure reading 99 missing parts 40 mixer 9 motor drive power 103 non operating altitude 35 non operating temperature 35 1260 Binary Pump VL User Manual 0 operating Altitude 35 operating temperature 35 optimization stack configuration 43 outlet valve 133 153 179 overview pump 9 P packaging damaged 40 parts identification cables 191 parts active inlet valve 181 bottle head assembly 185 damaged 40 hydraulic path with solvent selection valve 186 hydraulic path without solvent selection valve 188 missing 40 outlet valve 179 pump housing and main assemblies 174 pump housing and main assemblies 172 solvent cabinet 184 performance specification 36 pH range 36 physical specifications 35 piston 10 133 146 power supply indicator 81 power considerat
32. 6 Restriction capillary blocked at pre mixing Exchange restriction capillary union 1260 Binary Pump VL User Manual 103 7 Error Information Encoder Missing Encoder Missing Pump channel A B Encoder Missing Pump channel B The optical encoder on the pump motor in the module is missing or defective The processor checks the presence of the pump encoder connector every 2 seconds If the connector is not detected by the processor the error message is generated Probable cause Suggested actions 1 Defective or disconnected pump encoder Please contact your Agilent service connector representative 2 Defective pump drive assembly Please contact your Agilent service representative 104 1260 Binary Pump VL User Manual Error Information 7 Inlet Valve Missing Inlet Valve Missing Pump channel A B Inlet Valve Missing Pump channel B The active inlet valve in the module is missing or defective The processor checks the presence of the active inlet valve connector every 2 seconds If the connector is not detected by the processor the error message is generated Probable cause Suggested actions 1 Disconnected or defective cable Please contact your Agilent service representative 2 Disconnected or defective connection cable Please contact your Agilent service front panel to main board representative 3 Defective active inlet valve Exchange the active inlet valve 1260 Binary Pump VL User Manual 105 7 Error Information
33. 8 Leak Test Description The leak test is a built in troubleshooting test designed to demonstrate the leak tightness of the binary pump The test involves monitoring the pressure profile as the binary pump runs through a predefined pumping sequence The resulting pressure profile provides information about the pressure tightness and operation of the binary pump components see Figure 2 on page 10 Ramp 1 The test begins with the initialization of both pumps After initialization pistons Al and B1 are both at the top of their stroke Next the pump begins pumping solvent with a flow rate of 150 uL min stroke of 100 uL and a composition of 51 A 49 B Both pumps deliver for one complete pump cycle At the end of this step pistons Al and B1 are at the top of their stroke Ramp 2 The pump continues pumping solvent with a flow rate of 150 uL min Channel A delivers for one pump cycle first piston A2 delivers then piston A1 followed by channel B piston B2 then piston B1 both channels with a stroke of 20 uL Ramp 3 Just before the start of the first plateau piston A2 delivers with a flow rate of 50 uL min for approximately 8 s Plateau 1 At plateau 1 piston A2 delivers with a flow rate of 3 uL min for 30 s Ramp 4 Piston B2 delivers 50 uL min for approximately 8 s 1260 Binary Pump VL User Manual 121 122 Test Functions and Calibration Plateau 2 Piston B2 delivers with a flow rate of 3 uL min for 30 s
34. 8 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 212 1260 Binary Pump VL User Manual Appendix 12 Solvent Information Observe the following recommendations on the use of solvents e Brown glass ware can avoid growth of algae e Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters e Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid and nitric acid especially at higher temperatures if your chromatography method allows replace by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCly O gt gt 2COCl 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing stron
35. AN Bus cable AC Power Figure 11 Recommended Two Stack Configuration for 1260 Rear View 1260 Binary Pump VL User Manual 47 3 Installing the Pump Installing the Binary Pump Parts required Preparations p n Description 1 Pump 1 Data System 1 G4208A Instant Pilot 1 Power cord For other cables see text below and Cable Overview on page 192 Locate bench space Provide power connections Unpack the module Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 48 1260 Binary Pump VL User Manual Installing the Pump 3 Installing the Binary Pump 1 Place the module on the bench in a horizontal position 2
36. Ensure the power switches on the front of the modules are OFF switches stand out 3 At the rear of the binary pump move the security lever to its maximum right position Status lamp Power switch Serial number Figure 12 Front of Binary Pump 4 Connect the power cable to the power connector at the rear of the module The security lever will prevent that the cover is opened while the power cord is connected to the module 1260 Binary Pump VL User Manual 49 3 Installing the Pump Installing the Binary Pump 5 Connect the required interface cables to the rear of the binary pump see Connecting Modules and Control Software on page 51 Configuration switch GPIB Slot for interface board RS232 Remote Analog output CAN Bus Power plug Security lever Figure 13 Rear of Binary Pump 6 Connect the capillary solvent tubes and waste tubings see Flow Connections of the Binary Pump with Solvent Selection Valve on page 54 or Flow Connections of the Binary Pump Without Solvent Selection Valve on page 57 7 Press the power switch to turn on the module NOTE The power switch stays pressed in and a green indicator lamp in the power switch is on when the module is turned on When the line power switch stands out and the green light is off the module is turned off 8 Purge the binary pump see Priming and Purging the System on page 60 NOTE The pump was ship
37. It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations e the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 1260 Binary Pump VL User Manual 15 1 Introduction Electrical Connections 16 The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization One analog output provides signals for integrators or data handling systems The interface board slot is used for external contacts and BCD bottle number output or LAN connections The REMOTE connector may be used in combination with other analyt
38. OR FW UPDATE For use with G1323 Control Module 204 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 12 Appendix General Safety Information 206 The Waste Electrical and Electronic Equipment Directive 209 Batteries Information 210 Radio Interference 211 Sound Emission 212 Solvent Information 213 Agilent Technologies on Internet 214 This chapter provides addition information on safety legal and web ee Agilent Technologies 205 12 Appendix General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation 206 Before applying power comply with the installation section Additionally the following must be observed Do not
39. Performance 5 When to Remove the Static Mixer The binary pump is equipped with a static mixer The total delay volume of the pump is 600 900 ul The mixer has a volume of 420 ul The static mixer and both connecting capillaries can be replaced by a small capillary G1312 67301 under the following conditions e the delay volume of the pump should be reduced to a minimum for fastest gradient response and e the detector is used at medium or low sensitivity Removing the mixer will result in an increase of the composition ripple and higher detector noise 1260 Binary Pump VL User Manual 75 5 Optimizing Performance How to Optimize the Compressibility Compensation Setting 76 The compressibility compensation default settings are 50 x 10 bar best for most aqueous solutions for pump head A and 115 x 106 bar to suit organic solvents for pump head B The settings represent average values for aqueous solvents A side and organic solvents B side Therefore it is always recommended to use the aqueous solvent on the A side of the pump and the organic solvent on the B side Under normal conditions the default settings reduce the pressure pulsation to values below 1 of system pressure that will be sufficient for most applications If the compressibility values for the solvents used differ from the default settings it is recommended to change the compressibility values accordingly Compressibility settings can be optimiz
40. User Manual Parts for Maintenance 10 Active Inlet Valve Assembly Active Inlet Valve Assembly Item p n Description 1 G1312 60025 Active inlet valve without cartridge 2 5062 8562 Active Inlet Valve Cartridge 400 bar Figure 33 Active Inlet Valve Assembly 1260 Binary Pump VL User Manual 181 10 Parts for Maintenance Accessory Kit Accessory Kit p n G1312 68765 p n Description 0890 1195 Drain tube 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste 5181 1519 CAN cable Agilent module to module 1 m 5988 8453EN Capillary fitting starter kit brochure 9222 0519 Bag plastic G1312 87303 SS Capillary 400 x 0 17 mm m m ps ps G1312 87304 SST capillary 700 mm 0 17 mm i d 1 32 1 32 01200 60001 RRLC system configurator A 01 01 CD ROM G1311 90107 Algae note 5042 9954 4x Tubing clip 2x re order 4 pk G1311 60003 Bottle head assembly 4x 182 1260 Binary Pump VL User Manual Parts for Maintenance 10 Seal Wash Option G1312 68711 Table 22 Active Seal Wash Option kit for Binary pump Description Part Number Seal wash pump assembly includes pump casette and pump motor p n 5065 9953 Pump casette Silicone p n 5042 8507 Support ring seal wash 4 EA for Binary pump p n 5062 2465 Secondary seal pre installed in support rings p n 0905 1175 Gasket wash seal 4 EA for binary pump for re order pack of 6 p n 5062 2484 Seal keeper 4 EA for binary pump p n 5001 3743 Silicone rubber tub
41. a LAN cable which requires the installation of a LAN board For more information about connecting the Instant Pilot or Agilent control software refer to the respective user manual For connecting the Agilent 1260 Infinity equipment to non Agilent 1260 Infinity equipment see Introduction to the Binary Pump on page 8 1260 Binary Pump VL User Manual 53 3 Installing the Pump Flow Connections of the Binary Pump with Solvent Selection Valve Parts required p n Description 1 Other modules 1 G1311 68705 Accessory Kit 2 wrenches 1 4 5 16 inch for capillary connections Preparations Pump is installed in the LC system WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can bear health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1 Remove the front cover by pressing the snap fasteners on both sides Figure 14 Removing the Front Cover 54 1260 Binary Pump VL User Manual Installing the Pump 3 2 Place the solvent cabinet on top of the binary pump 3 Place the bottles into the solvent cabinet and place a bottle head assembly into each bottle 4 Connect the solvent tubes from the bottle head assemblies to the inlet connecto
42. alve SST gold PTFE ceramic Damping unit Gold SST For pump specifications see Performance Specifications on page 36 1260 Binary Pump VL User Manual Introduction 1 How Does Compressibility Compensation Work The compressibility of the solvents in use will affect retention time stability when the back pressure in the system changes for example ageing of column In order to minimize this effect the pump provides a compressibility compensation feature which optimizes the flow stability according to the solvent type The compressibility compensation is set to a default value and can be changed through the user interface Without a compressibility compensation the following will happen during a stroke of the first piston The pressure in the piston chamber increases and the volume in the chamber will be compressed depending on backpressure and solvent type The volume displaced into the system will be reduced by the compressed volume With a compressibility value set the processor calculates a compensation volume that is depending on the backpressure in the system and the selected compressibility This compensation volume will be added to the normal stroke volume and compensates the previous described loss of volume during the delivery stroke of the first piston How Does Variable Stroke Volume Work Due to the compression of the pump chamber volume each piston stroke of the pump will generate a small pressure pulsation
43. always on top or at a higher level of the binary pump When using the binary pump without vacuum degasser shortly degass your solvents for example apply vacuum for 15 30 s in an appropriate vessel before using them in the binary pump If possible apply solvent conditions that will decrease the gas solubility over time for example warming up the solvents For highest precision and reproducibility use a vacuum degasser When using the binary pump with vacuum degasser before operating the binary pump flush the degasser with at least two degasser tubing volumes 80 mL especially when the pumping system was turned off for a certain length of time for example overnight and volatile solvent mixtures are used in the channels see Priming and Purging the System on page 60 Prevent blocking of solvent inlet filters never use the pump without solvent inlet filter Growth of algae should be avoided see Prevent Blocking of Solvent Filters on page 67 Check purge valve frit and column frit in regular time intervals A blocked purge valve frit can be identified by black or yellow layers on its surface or by a pressure greater than 10 bar when pumping distilled water at a rate of 5 mL min with an open purge valve When using the binary pump at low flow rates below 0 2 mL min check all 1 16 inch fittings for any signs of leaks Whenever possible use a minimum flow rate of 5 uL min per solvent channel to avoid crossf
44. ance 71 When to Use a Vacuum Degasser 72 When to Use the Seal Wash Option active seal wash 73 Choosing the Right Pump Seals 74 When to Remove the Static Mixer 75 How to Optimize the Compressibility Compensation Setting 76 Troubleshooting and Diagnostics 79 Status Indicators 80 Status Indicators 81 User Interfaces 83 Agilent Lab Advisor Software 84 Error Information 85 What Are Error Messages 87 General Error Messages 88 Module Error Messages 94 Test Functions and Calibration 115 Pressure Test 116 Leak Test 121 Maintenance 129 Introduction to Maintenance and Repair 130 Warnings and Cautions 131 Overview of Maintenance and Repair 132 Cleaning the Module 134 Checking and Cleaning the Solvent Filter 135 Exchanging the Purge Valve Frit or the Purge Valve Removing the Pump Head Assembly 140 Disassembling the pump head 142 Exchanging the Pump Seals 143 Seal Wear in Procedure 145 Exchanging the Plungers 146 Exchanging the Wash Seals 147 Reinstalling the Pump Head Assembly 149 137 1260 Binary Pump VL User Manual Contents Exchanging the Inlet Valve 151 Exchanging the Outlet Valve 153 Installation of the Solvent Selection Valve Upgrade Kit 155 Exchanging the Solvent Selection Valve 157 Installing the Active Seal Wash Option 160 Exchanging the Active Inlet Valve 164 Exchanging the Active Inlet Valve Cartridge 166 Exchanging the Optional Interface Board 168 Replacing the Module s Firmware 170 10 P
45. anual 67 4 Using the Binary Pump Algae Growth in HPLC Systems 68 The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems Algae grow in aqueous media preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light will also stimulate their growth Even in distilled water small sized algae grow after some time Instrumental Problems Associated With Algae Algae deposit and grow everywhere within the HPLC system causing e Deposits on ball valves inlet or outlet resulting in unstable flow or total failure of the pump e Small pore solvent inlet filters to plug resulting in unstable flow or total failure of the pump Small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure e Column filters to plug giving high system pressure e Flow cell windows of detectors to become dirty resulting in higher noise levels since the detector is the last module in the flow path this problem is less common Symptoms Observed with the Agilent 1260 Infinity HPLC In contrast to the HP 1090 and HP 1050 Series HPLC systems which use helium degassing algae have a better chance to grow in systems such as the Agilent 1260 Infinity where helium is not used for degassing most algae need oxygen and light for gro
46. ap 8 Insert the purge valve into the purge valve holder and orient he waste outlet nozzle downward as shown below 138 1260 Binary Pump VL User Manual Maintenance 9 Exchanging the Purge Valve Frit or the Purge Valve 9 Tighten the purge valve and reconnect outlet capillary and waste tubing Pump outlet capillary Purge valve Waste tube Figure 20 Exchanging the Purge Valve 1260 Binary Pump VL User Manual 139 9 Maintenance Removing the Pump Head Assembly When Tools required Preparations CAUTION Exchanging pump seals Exchanging pistons Exchanging seals of the seal wash option Wrench 1 4 inch 3 mm hexagonal key 4 mm hexagonal key 1 4 inch slitted socket wrench Switch off the pump at the main power switch Make sure that the pump head is not removed This may damage the pump drive gt Never start the pump when the pump head is removed 140 Both pump head assemblies use the same internal components In addition pump head A is fitted with the purge valve The following procedure describes the removal and disassembly of pump head A left For pump head B right proceed in the same way and skip steps that deal with the purge valve 1 Remove the front cover 2 2 Disconnect the capillaries at the back of the purge valve holder the pump head adapter and the tube at the inlet valve Beware of leaking solvents 1260 Binary Pump VL User Manual Maintenance 9 Removing the Pump Head Assembly
47. arts for Maintenance 171 Parts and Materials 172 Pump Head Assembly Without Seal Wash 175 Pump Head Assembly with Seal Wash Option 177 Outlet Valve 179 Purge Valve Assembly 180 Active Inlet Valve Assembly 181 Accessory Kit 182 Seal Wash Option G1312 68711 183 Solvent Cabinet 184 Bottle Head Assembly 185 Hydraulic Path with Solvent Selection Valve 186 Hydraulic Path without Solvent Selection Valve 188 1 Identifying Cables 191 Cable Overview 192 Analog Cables 194 Remote Cables 196 BCD Cables 199 CAN LAN Cables 201 External Contact Cable 202 Agilent Module to PC 203 Agilent 1200 Module to Printer 204 1260 Binary Pump VL User Manual Contents 12 Appendix 205 General Safety Information 206 The Waste Electrical and Electronic Equipment Directive 209 Batteries Information 210 Radio Interference 211 Sound Emission 212 Solvent Information 213 Agilent Technologies on Internet 214 6 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 1 Introduction Introduction to the Binary Pump 8 Overview of the Binary Pump 9 How Does the Binary Pump Work 10 How Does Compressibility Compensation Work 13 How Does Variable Stroke Volume Work 13 Early Maintenance Feedback 14 Instrument Layout 15 Electrical Connections 16 Serial Number Information ALL 17 Electrical Connections 16 Interfaces 18 Overview Interfaces 20 Setting the 8 bit Configuration Switch On Board LAN 24 Communication Setting
48. arts required Description G1381 60000 Solvent Selection Valve Upgrade Kit Preparations Switch off the pump at the main power switch 1 Lift solvent bottles out of the solvent cabinet and place 2 Disconnect all tubings from the solvent selection valve them on the table Disconnect the solvent tubes from the solvent selection valve and empty the tubes into the bottles Place the bottles back into the solvent cabinet 1260 Binary Pump VL User Manual 157 9 Maintenance Exchanging the Solvent Selection Valve 3 Using a Pozidriv screwdriver 1 loosen the holding screws of the valve holder 4 Carefully pull the valve holder out and disconnect the valve cable at the connector 6 Tighten the screws of the valve holder 158 1260 Binary Pump VL User Manual Maintenance 9 Exchanging the Solvent Selection Valve 7 Reconnect all tubings to the solvent selection valve NOTE After an exchange of the valve it may be required to pump several mL of solvent before the flow stabilizes at a pressure ripple as low as it used to be when the system was still working properly 1260 Binary Pump VL User Manual 159 9 Maintenance Installing the Active Seal Wash Option When When installing seal wash option Tools required 4 mm hexagonal key Screwdriver Pozidrive 1 Parts required p n Description 1 G1311 68711 Active Seal Wash Option kit Isocratic or Quaternar
49. cable Use a solvent shutoff valve or lift up solvent filters for avoiding leakages Remove the front cover 2 Unplug the active inlet valve cable from the connector Disconnect the solvent inlet tube at the inlet valve beware of leaking solvents BPs without the solvent selection valve SSV have an adapter installed between the solvent line and the active inlet valve AIV Disconnect the solvent tubes at the adapter and remove the adapter from the AIV Using a 14 mm wrench loosen the active inlet valve and remove the valve from pump head Using a pair of tweezers remove the valve cartridge from the actuator assembly Clean the area in the actuator assembly Flush the cartridge area thouroughly Insert a new cartridge into the actuator assembly make sure the valve cartridge is completely inserted into the actuator assembly Insert the valve into the pump head Using the 14 mm wrench turn the nut until it is hand tight Position the valve so that the solvent inlet tube connection points towards the front 1260 Binary Pump VL User Manual Maintenance 9 10 Using the 14 mm wrench tighten the nut by turning the valve in its final position do not overtighten the valve 11 Reconnect the Active Inlet Valve cable to the connector at the Z panel and the inlet tube to the valve 12 Reinstall the front cover Active inlet valve body Valve cartridge Figure 26 Active Inlet Valve Parts After an exchange of the va
50. ces its lifetime The seal will build up sticky layers on the surface of the piston These sticky layers will also reduce the lifetime of the pump seal Therefore the tubes of the wash option should always be filled with solvent to prolong the lifetime of the wash seal Always use a mixture of LC grade water 90 and isopropanol 10 as wash solvent This mixture prevents growth of algae or bacteria in the wash bottle and reduces the surface tension of the water For information on the installation of the active seal wash option refer to Installing the Active Seal Wash Option on page 160 1260 Binary Pump VL User Manual 73 5 Optimizing Performance Choosing the Right Pump Seals The standard seal for the pump can be used for most applications However applications that use normal phase solvents for example hexane are not suited for the standard seal and require a different seal when used for a longer time in the pump For applications that use normal phase solvents for example hexane we recommend using polyethylene pump seals PE seals pack of 2 p n 0905 1420 and Wash Seal PE p n 0905 1718 These seals have less abrasion compared to the standard seals Polyethylene seals have a limited pressure range of 0 200 bar When used above 200 bar their lifetime is reduced significantly DO NOT apply the seal wear in procedure performed with new standard seals at 400 bar 74 1260 Binary Pump VL User Manual Optimizing
51. ctance motor drives the two ball screw drives in opposite directions The gears for the ball screw drives have different circumferences ratio 2 1 allowing the first piston to move at double the speed of the second piston The solvent enters the pump heads close to the bottom limit and leaves it at its top The outer diameter of the piston is smaller than the inner diameter of the pump head chamber allowing the solvent to fill the gap in between The first piston has a stroke volume in the range of 20 ul to 100 ul depending on the flow rate The microprocessor controls all flow rates in a range of 1 ul min to 5 ml min The inlet of the first pumping unit is connected to the active inlet valve which is processor controlled opened or closed allowing solvent to be drawn into the first pump unit The outlet of the pump unit is connected directly to the second pump unit The outlet of the second pump unit is connected via a small mixing chamber a coil 1260 Binary Pump VL User Manual 11 1 12 Introduction and the damping unit to the purge valve assembly The outlet of the purge valve assembly is then connected to the following chromatographic system When turned on the pump runs through an initialization procedure to determine the upper dead center of the first piston of both pump channels The first piston moves slowly upwards to the mechanical stop of the pump head and from there it moves back a predetermined path length The controller stor
52. d Repair 130 Warnings and Cautions 131 Overview of Maintenance and Repair 132 Cleaning the Module 134 Checking and Cleaning the Solvent Filter 135 Exchanging the Purge Valve Frit or the Purge Valve 137 Removing the Pump Head Assembly 140 Disassembling the pump head 142 Exchanging the Pump Seals 143 Seal Wear in Procedure 145 Exchanging the Plungers 146 Exchanging the Wash Seals 147 Reinstalling the Pump Head Assembly 149 Exchanging the Inlet Valve 151 Exchanging the Outlet Valve 153 Installation of the Solvent Selection Valve Upgrade Kit 155 Exchanging the Solvent Selection Valve 157 Installing the Active Seal Wash Option 160 Exchanging the Active Inlet Valve 164 Exchanging the Active Inlet Valve Cartridge 166 Exchanging the Optional Interface Board 168 Replacing the Module s Firmware 170 This chapter describes the maintenance of the module a Agilent Technologies 129 9 Maintenance Introduction to Maintenance and Repair The module is designed for easy repair The most frequent repairs such as piston seal change and purge valve frit change can be done from the front of the module with the module in place in the system stack These repairs are described in Simple Repairs on page 133 130 1260 Binary Pump VL User Manual Maintenance 9 Warnings and Cautions Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When w
53. ds that Agilent Technologies supplies with this instrument for any other equipment 1260 Binary Pump VL User Manual 33 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 13 on page 35 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench should carry an Agilent system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 34 1260 Binary Pump VL User Manual Site Requirements and Specifications 2 Physical Specifications Table 13 Physical Specifications Type Specification Comments Weight 15 5 kg 34 Ibs Dimensions height x 180 x 345 x 435 mm 7 x 13 5 x 17 inches width x depth Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 220 VA 74 W 253 BTU Maximum Ambient ope
54. dule Liquid in the module electronics can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any fittings 134 1260 Binary Pump VL User Manual Maintenance 9 Checking and Cleaning the Solvent Filter When Parts required Preparations CAUTION If solvent filter is blocked Description Concentrated nitric acid 35 LC grade water Beaker Remove solvent inlet tube from the adapter at the AIV Small particles can permanently block the capillaries and valves of the module Damage of the module gt Always filter solvents gt Never use the module without solvent inlet filter If the filter is in good condition the solvent will freely drip out of the solvent tube hydrostatic pressure If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can bear health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1260 Binary Pump VL User Manual 135 9 Maintenance Cleaning the Solvent Filter 1 Remove the blocked solvent filter from the bottle
55. dule to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground gt NC 2 Brown Prepare run Low e 3 3 Gray Start Low m s NC 4 Blue Shut down Low e O a NC 5 Pink Not connected NC 6 Yellow Power on High o 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 A D Pin Agilent Signal Name Active module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 1260 Binary Pump VL User Manual 197 11 Identifying Cables Agilent Module to General Purpose p n 01046 60201 Pin Universal Pin Agilent Signal Name Active module TTL 1 White Digital ground A O 1 2 B oo Brown Prepare run Low o0 oo og 3 Gray Start Low KEY coal oo BG 4 Blue Shut down Low o0 am 5 Pink Not connected oOo 0 ji 6 Yellow Power on High O 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 198 1260 Binary Pump VL User Manual BCD Cables
56. e 61 for the other channel s of the binary pump When priming the vacuum degasser with a syringe the solvent is drawn through the degasser tubes very quickly The solvent at the degasser outlet will therefore not be fully degassed Pump for approximately 10 minutes with your selected flow rate before starting any application This will allow the vacuum degasser to properly degas the solvent in the degasser tubes The pump should never be used for priming empty tubings never let the pump run dry Use the syringe to draw enough solvent for completely filling the tubings to the pump inlet before continueing to prime with the pump 1260 Binary Pump VL User Manual 61 3 62 Installing the Pump Priming with the Pump When the pumping system has been turned off for a certain time for example overnight oxygen will rediffuse into the solvent channel between the vacuum degasser and the pump Solvents containing volatile ingredients will slightly lose these if left in the degasser without flow for a prolonged period of time Therefore priming of the vacuum degasser and the pumping system is required before starting an application 1 Open the purge valve of your pump by turning it counterclockwise and set flow rate to 3 5 ml min Flush the vacuum degasser and all tubes with at least 30 ml of solvent Set flow to required value of your application and close the purge valve Pump for approximately 10 minutes before starting your applicat
57. e inlet valve 2 Defective connection cable front panel to Please contact your Agilent service main board representative 3 Defective main board Please contact your Agilent service representative Temperature Out of Range Temperature Out of Range 0 Pump channel A Temperature Out of Range 1 Pump channel B One of the temperature sensor readings in the motor drive circuit are out of range The values supplied to the ADC by the hybrid sensors must be between 0 5 V and 4 3 V If the values are outside this range the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative 1260 Binary Pump VL User Manual 101 7 Error Information Temperature Limit Exceeded Temperature Limit Exceeded 0 Pump channel A Temperature Limit Exceeded 1 Pump channel B The temperature of one of the motor drive circuits is too high The processor continually monitors the temperature of the drive circuits on the main board If excessive current is being drawn for long periods the temperature of the circuits increases If the temperature exceeds the upper limit the error message is generated Probable cause Suggested actions 1 High friction partial mechanical blockage in Ensure the capillaries and frits between the the pump drive assembly pump head and damper inlet are free from blockage 2 Partial blockage of the flowpath in front of Ensure the outlet valve is no
58. e is generated Probable cause Suggested actions 1 Pump head not installed correctly screws Install the pump head correctly Ensure nothing not secured or pump head not seated e g capillary is trapped between the pump correctly head and body 2 Broken piston Exchange the piston 1260 Binary Pump VL User Manual 107 7 Error Information Index Limit Index Limit Pump channel A B Index Limit Pump channel B The time required by the piston to reach the encoder index position was too short pump During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is reached too fast the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Please contact your Agilent service representative 108 1260 Binary Pump VL User Manual Error Information 7 Index Adjustment Index Adjustment Pump channel A B Index Adjustment Pump channel B The encoder index position in the module is out of adjustment During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encode
59. ed actions 1 Active inlet valve and pump encoder of Reconnect the active inlet valve and pump channel B disconnected encoder of channel B 1260 Binary Pump VL User Manual 99 7 Error Information Valve Fuse Following errors can only occur if a solvent selection valve is used The internal number in the error message is linked either to channels A or B as shown in the following table Valve Fuse 0 Channels Al and A2 Valve Fuse 1 Channels B1 and B2 One of the solvent selection valves in the pump has drawn excessive current causing the selection valve electronic fuse to open Probable cause Suggested actions 1 Defective solvent selection valve Restart the capillary pump If the error message appears again exchange the solvent selection valve 2 Defective connection cable front panel to Please contact your Agilent service main board representative 3 Defective main board Please contact your Agilent service representative 4 1200 Series solvent selection valve installed Replace by 1260 solvent selection valve 100 1260 Binary Pump VL User Manual Error Information 7 Inlet Valve Fuse Inlet Valve Fuse 0 Pump channel A Inlet Valve Fuse 1 Pump channel B One of the active inlet valves in the module has drawn excessive current causing the inlet valve electronic fuse to open Probable cause Suggested actions 1 Defective active inlet valve Restart the module If the error message appears again exchange the activ
60. ed by using the values for various solvents described in Table 17 on page 77 If the solvent in use is not listed in the compressibility table when using premixed solvents and if the default settings are not sufficient for your application the following procedure can be used to optimize the compressibility settings 1 Start channel A of the binary pump with the required flow rate 2 Before starting the optimization procedure the flow must be stable Use degassed solvent only Check the tightness of the system with the pressure test see Description on page 116 3 Your pump must be connected to a data system or Instant Pilot with which the pressure and ripple can be monitored otherwhise connect a signal cable between the pressure output of the pump and a recording device for example 339X integrator and set parameters Zero 50 Att 2 3 Chart Speed 10 cm min 4 Start the recording device with the plot mode 5 Starting with a compressibility setting of 10 x 10 bar increase the value in steps of 10 Re zero the integrator as required The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition 1260 Binary Pump VL User Manual Optimizing Performance 5 Table 17 Solvent Compressibility Solvent pure Compressibility 10 bar Acetone 126 Acetonitrile 115 Benzene 95 Carbon tetrachloride 110 Chloroform 100 Cyclohexane 118 Ethanol 114 Et
61. efine that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again in order to store the values in the non volatile memory Table 7 Communication Settings for RS 232C Communication without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up 1260 Binary Pump VL User Manual 27 1 28 Introduction Table 8 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table9 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication Table 10 Parity Settings without on board LAN Switches Parity 7 8 0 0 No Parity 1 0 Odd Parity 1 1 Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity 1260 Binary Pump VL User Manual Introduction 1 Special Settings The special settings are required for specific actions normally in a service case The tables include both settings for modules with on board LAN and
62. election Valve Parts required p n Description 1 Other modules 1 G1311 68705 Accessory Kit 2 wrenches 1 4 5 16 inch for capillary connections Preparations Pump is installed in the LC system WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can bear health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1 Remove the front cover by pressing the snap fasteners on both sides Figure 16 Removing the Front Cover 2 Place the solvent cabinet on top of the binary pump 1260 Binary Pump VL User Manual 57 3 58 Installing the Pump Place the bottles into the solvent cabinet and place a bottle head assembly into each bottle Connect the solvent tubes from the bottle head assemblies to the inlet adapters of the active inlet valves Fix the tubes in the clips of solvent cabinet and binary pump Using a piece of sanding paper connect the waste tubing to the purge valve and place it into your waste system If the binary pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack connect the corrugated waste tube to the waste outlet of the pump leak handling system Connect the outlet capillar
63. ents may leak out The handling of toxic and hazardous solvents and reagents can bear health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 60 Before using a new degasser or new tubings for the first time 1 Prime all tubings with at least 30 ml of iso propanol no matter whether the channels will be used with organic mobile phase or with water If you are changing to a solvent that is immiscible with the solvent currently in the tubing continue as follows 1260 Binary Pump VL User Manual Installing the Pump 3 2 Replace the current solvent with adequate organic solvent see Table 16 on page 62 if current solvent is organic or with water if current solvent is an inorganic buffer or contains salt Disconnect solvent tube from your pump Connect syringe adapter to solvent tube Push syringe adapter onto syringe ao ol A Q Slowly pull the syringe piston to draw at least 30 mL of solvent through degasser and tubing Replace the priming solvent with the new solvent of your choice 8 Pull syringe piston to draw at least 30 mL of solvent through degasser and tubing 9 Disconnect syringe adapter from solvent tube 10 Connect solvent tube to your pump 11 Repeat step 3 on page 61 through step 10 on pag
64. equest to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces Some modules have module specific interfaces connectors They are described in the module documentation 1260 Binary Pump VL User Manual 23 1 Introduction Setting the 8 bit Configuration Switch On Board LAN The 8 bit configuration switch is located at the rear of the module Switch settings provide configuration parameters for LAN serial communication protocol and instrument specific initialization procedures All modules with on board LAN e g G1315 65C D G1314D E F G4212A B G4220A e Default is ALL
65. er Manual 131 9 Maintenance Overview of Maintenance and Repair Overview of Maintenance and Repair Figure 19 on page 132 shows the main assemblies of the binary pump The pump heads and its parts do require normal maintenance for example seal exchange and can be accessed from the front simple repairs Replacing internal parts will require to remove the module from its stack and to open the top cover Figure 19 Overview of Repair Procedures 2 Inlet valve see Exchanging the Inlet Valve on page 151 4 Outlet valve see Exchanging the Outlet Valve on page 153 10 Solvent selection valve see Exchanging the Solvent Selection Valve on page 157 132 1260 Binary Pump VL User Manual Simple Repairs Maintenance The procedures described in this section can be done with the binary pump in place in the system stack Table 19 Simple Repair Procedures Procedure Typical Condition Notes Checking and Cleaning the Solvent Filter on page 135 Exchanging the Active Inlet Valve on page 164 Exchanging the Outlet Valve on page 153 Exchanging the Purge Valve Frit or the Purge Valve on page 137 Exchanging the Purge Valve Frit or the Purge Valve on page 137 Exchanging the Solvent Selection Valve on page 157 Exchanging the Pump Seals on page 143 Exchanging the Plungers on page 146 Exchanging the Wash Seals on page 147 Exchanging the O
66. es this piston position in memory After this initialization the pump starts operation with the set parameters for the two pump channels The active inlet valve is opened and the down moving piston draws solvent into the first pump head At the same time the second piston is moving upwards delivering into the system After a controller defined stroke length depending on the flow rate the drive motors are stopped and the active inlet valve is closed The motor direction is reversed and moves the first piston up until it reaches the stored upper limit and at the same time moving the second piston downwards Then the sequence starts again moving the pistons up and down between the two limits During the upward movement of the first piston the solvent in the pump head is pressed through the outlet ball valve into the second pumping unit The second piston draws in half of the volume displaced by the first piston and the remaining half volume is directly delivered into the system During the drawing stroke of the first piston the second piston delivers the drawn volume into the system Table 1 Pump Details Delay volume From mixing point to pump outlet dependent on back pressure 180 480 uL without mixer 600 900 uL with mixer Materials in contact with mobile phase Pump head SST gold sapphire ceramic Active inlet valve SST sapphire ruby ceramic PTFE Outlet valve SST gold sapphire ruby tantalum Adapter SST gold Purge v
67. et valve cable from the connector 3 Disconnect the solvent inlet tube at the inlet valve beware of leaking solvents BPs without the solvent selection valve SSV have an adapter installed between the solvent line and the active inlet valve AIV Disconnect the solvent tubes at the adapter and remove the adapter from the AIV 4 Using a 14 mm wrench loosen the active inlet valve and remove the valve from pump head 5 Insert the valve into the pump head Using the 14 mm wrench turn the nut until it is hand tight 6 Position the valve so that the solvent inlet tube connection points towards the front 1260 Binary Pump VL User Manual Maintenance 9 Exchanging the Active Inlet Valve 7 Using the 14 mm wrench tighten the nut by turning the valve in its final position not more than a quarter turn Do not overtighten the valve Solvent inlet tube Active inlet valve Figure 25 Exchanging the Active Inlet Valve 8 Reconnect the Active Inlet Valve cable to the connector at the Z panel and the inlet tube to the valve 9 Reinstall the front cover 1260 Binary Pump VL User Manual 165 9 Maintenance Exchanging the Active Inlet Valve Cartridge When Tools required Parts required Preparations 166 If internally leaking backflow Wrench 14 mm p n Description 1 1 G1312 60025 Active inlet valve body 5062 8562 Valve cartridge 400 bar Switch off pump at the main power switch and unplug the power
68. evel in the bottle falls below the specified value the error message appears when the feature is configured accordingly Probable cause Suggested actions 1 Volume in bottle below specified volume Refill bottles and reset solvent counters 2 Incorrect setting Make sure the limits are set correctly 1260 Binary Pump VL User Manual Error Information 7 Pressure Above Upper Limit The system pressure has exceeded the upper pressure limit Probable cause Suggested actions 1 Upper pressure limit set too low Ensure the upper pressure limit is set to a value suitable for the analysis 2 Blockage in the flowpath after the damper Check for blockage in the flowpath The following components are particularly subject to blockage inline filter frit needle autosampler seat capillary autosampler sample loop autosampler column frits and capillaries with small internal diameters e g 50 pm ID 3 Defective damper Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative 1260 Binary Pump VL User Manual 95 7 Error Information Pressure Below Lower Limit The system pressure has fallen below the lower pressure limit Probable cause Suggested actions 1 Lower pressure limit set too high Ensure the lower pressure limit is set to a value suitable for the analysis 2 Air bubbles in the mobile phase Ensure solvents are degassed Purge the module E
69. fastener 54 57 solvent cabinet 55 58 solvent filters checking 135 cleaning 136 solvent inlet filter 64 solvent selection valve 8 54 157 218 solvent cabinet 64 184 status indicators 80 81 solvent filters prevent blocking 67 U solvent information 66 unpacking the pump 40 solvents 213 sonic bath 153 v sound emission 212 vacuum degasser when to use 72 special ueg a vacuum degasser 37 special settings boot resident 29 forced cold start 29 vacuum degasser operational hints 72 vacuum degasser 8 64 valve specificati ae en 36 solvent selection 157 sine 35 variable stroke volume 13 ae variable reluctance motor 11 specifications 31 stack configuration 47 rearview 47 voltage range 35 static mixer 75 Ww status indicator 82 wait timeout 113 stroke length 111 wash seals 133 stroke volume 11 13 wash seals 147 synchronization lost 89 waste syringe adapter 61 electrical and electronic equipment 209 43 WEEE directive 209 weight 35 T wrench 1 4 inch slitted socket 140 wrench 1 4 inch 118 123 137 wrench 14mm 137 151 153 164 166 system setup and installation optimizing stack configuration temperature limit exceeded 102 temperature out of range 101 temperature sensor 92 test functions 80 timeout 88 tools screwdriver pozidriv 1 140 screwdriver pozidriv 1 157 wrench 1 4 inch slitted socket 140 wrench 14mm 153 troubleshooting error messages 80 87 1260 Binary Pump VL User Manual www ag
70. follow the instructions Make sure to release the pressure by slowly opening the purge valve when the test has finished System Pressure Test failed on page 120 describes the evaluation and interpretation of the pressure test results For detailed instructions refer to the Agilent Lab Monitor amp Diagnostic Software 1260 Binary Pump VL User Manual Test Functions and Calibration 8 Evaluating the Results The sum of all leaks between the pump and the blank nut will be indicated by a pressure drop of gt 2 bar minute at the plateau Note that small leaks may cause the test to fail but solvent may not be seen leaking from a module Please notice the difference between an error in the test and a failure of the test An error means that during the operation of the test there was an abnormal termination If a test failed this means that the results of the test were not within the specified limits If the pressure test fails e Ensure all fittings between the pump and the blank nut are tight Repeat the pressure test Often it is only a damaged blank nut itself poorly shaped from overtightening that causes a failure of the test Before investigating any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened e Ifthe test fails again insert the blank nut at the outlet of the previous module in the stack eg autosampler port 6 of the injection valve and re
71. g complexing agents e g EDTA Mixtures of carbon tetrachloride with 2 propanol or THF 1260 Binary Pump VL User Manual 213 12 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the modules for download 214 1260 Binary Pump VL User Manual Index Index solvent zero counter 94 8 bit configuration switch on board LAN 24 without On Board LAN 26 A active inlet valve 164 164 active inlet valve 133 166 181 active sealwash 8 73 adapter 58 164 166 Agilent Diagnostic software 84 Agilent Lab Advisor software 84 Agilent Lab Advisor 84 Agilent oninternet 214 algae growth 67 algae 64 213 alternative seal material 74 ambient operating temperature 35 ambient non operating temperature 35 analog output 37 analog signal 21 analog cable 194 apg remote 22 assembly bottle head 185 AUTO mode 13 AUX output 52 1260 Binary Pump VL User Manual ball screw drive 10 battery safety information 210 BCD board 168 BCD cable 199 bench space 34 binary pump checklist 40 blockage 103 bottle head assembly 185 185 buffer application 64 buffer solutions 8 C cable analog 194 BCD 199 CAN 201 connecting APG remote 47 connecting CAN 47 connecting GPIB 47 connecting LAN 47 connecting the ChemStation 47 connecting the p
72. g surface from scratches Place the valve in upright position sitting on the plastic cap in a small beaker with premixed water isopropanol 50 50 Sonicate for 5 to 10 minutes Replace the gold seal 1 Using a 1 4 inch wrench disconnect the valve capillary from the outlet valve 2 Unscrew the valve with the 14 mm wrench and remove it from the pump body 3 In case you are only sonicating the valve check the plastic cap and the gold seal for damage Check the gold seal It should be exchanged when heavily deformed Inspect the cap and replace it with a new one if cracks are visible 4 Reinstall the outlet valve and tighten it 1260 Binary Pump VL User Manual 153 9 Maintenance Exchanging the Outlet Valve 5 Reconnect the valve capillary Absorber capillary Outlet valve 154 1260 Binary Pump VL User Manual Maintenance 9 Installation of the Solvent Selection Valve Upgrade Kit When Tools required Parts required Preparations A solvent selection valve allows you choosing between 4 different solvents that can be used with a binary pump The valve switches between two solvents Al and A2 for channel A of the left pump head and two solvents B1 and B2 for channel B of the right pump head Applicable modules This kit is compatible to the 1260 Binary Pumps G1312B and G1312C Screwdriver Pozidriv 1 Description G1381 60000 Solvent Selection Valve Upgrade Kit If required remove solvent tubes from the inlet va
73. genated solvents or mixtures which form radicals and or acids for example 2CHCl Og gt 2COCly 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Mixtures of carbon tetrachloride with 2 propanol or THF dissolve stainless steel 1260 Binary Pump VL User Manual Using the Binary Pump 4 Prevent Blocking of Solvent Filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module This is especially true for aqueous solvents or phosphate buffers pH 4 to 7 The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the module e Use a sterile if possible amber solvent bottle to slow down algae growth e Filter solvents through filters or membranes that remove algae e Exchange solvents every two days or refilter e Ifthe application permits add 0 0001 0 001M sodium azide to the solvent e Place a layer of argon on top of your solvent e Avoid exposure of the solvent bottle to direct sunlight Never use the system without solvent filter installed 1260 Binary Pump VL User M
74. generic to all Agilent series HPLC modules and may show up on other modules as well The timeout threshold was exceeded Probable cause 1 The analysis was completed successfully and the timeout function switched off the module as requested 2 Anot ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Shut Down Suggested actions Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required An external instrument has generated a shut down signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system 4 The degasser failed to generate sufficient vacuum for solvent degassing Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition Check the vacuum degasser for
75. gh pressure side are performed by two pump assemblies which can generate pressure up to 400 bar Each channel comprises a pump assembly including pump drive pump head active inlet valve which has a replaceable cartridge and outlet valve Both channels are connected in a low volume mixing chamber which is connected by a capillary coil to a damping unit and a mixer A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pumping system 1260 Binary Pump VL User Manual 9 1 introduction Overview of the Binary Pump Purge valve Pump outlet Mixer lt To waste Outlet valve Outlet valve Inlet valve From From solvent bottle solvent bottle Seal Piston Pump head A Pump head B Figure2 The Hydraulic Path A seal wash optional is available when the pump is used with buffer solutions How Does the Binary Pump Work The liquid runs from the solvent reservoir through an active inlet valve Each side of the binary pump comprises two substantially identical pump units Both pump units comprise a ball screw drive and a pump head with two sapphire pistons for reciprocating movement 10 1260 Binary Pump VL User Manual Introduction 1 Overview of the Binary Pump To mixing chamber From solvent bottle Piston Ball screw drive Motor with encoder Figure 3 Principle of Pump Unit A servo controlled variable relu
76. he leak test results Detailed instructions are provided in the Lab Advisor Software 1260 Binary Pump VL User Manual 123 8 Test Functions and Calibration Evaluating the Results Defective or leaky components in the pump head lead to changes in the Leak Test pressure plot Typical failure modes are described below Please notice the difference between an error in the test and a failure of the test An error means that during the operation of the test there was an abnormal termination If a test failed this means that the results of the test were not within the specified limits Often it is only the damaged blank nut itself poorly shaped from overtightening that causes a failure of the test Before investigating on any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened No pressure increase or minimum pressure of plateau 1 not reached Probable cause Suggested actions 1 Pump not running Check the logbook for error messages 2 Purge valve open Close the purge valve and restart the test 3 Wrong solvent line connections to solvent Ensure the solvent lines from the degasser to selection valve the solvent selection valve are connected correctly 4 Loose or leaky fittings Ensure all fittings are tight or exchange capillary 5 Large leaks visible at the pump seals Exchange the pump seals 6 Large leaks visible at active inlet valve Ensure the leaky componen
77. head assembly and place it in a beaker with concentrated nitric acid 35 for one hour 2 Thoroughly flush the filter with LC grade water remove all nitric acid some columns can be damaged by concentrated nitric acid 3 Reinstall the filter 136 1260 Binary Pump VL User Manual Maintenance 9 Exchanging the Purge Valve Frit or the Purge Valve When Frit when plunger seals are exchanged or when contaminated or blocked pressure drop of gt 10 bar across the frit at a flow rate of 5 mL min of H20 with purge valve opened Purge valve if purge valve cannot be closed leak tight Tools required Wrench 1 4 inch Wrench 14mm Pair of tweezers or toothpick Parts required p n Description 1 01018 22707 PTFE frit pack of 5 1 G1312 60061 Purge valve assembly 1 Using a 1 4 inch wrench disconnect the pump outlet capillary at the purge valve 2 Disconnect the waste tube Beware of leaking solvents due to hydrostatic pressure 3 Using the 14 mm wrench unscrew the purge valve and remove it from the purge valve holder 4 Remove the plastic cap with the gold seal from the purge valve 1260 Binary Pump VL User Manual 137 9 Maintenance 5 Use a pair of tweezers or a toothpick to remove the frit 1 Valve body Purge valve assembly p n G1312 60061 2 PTFE frit pack of 5 p n 01018 22707 3 Seal cap p n 5067 4728 6 Place a new frit into the purge valve with the slit facing the seal cap 7 Replace the seal c
78. hort 91 Fan Failed 92 Leak 92 OpenCover 93 Module Error Messages 94 Restart Without Cover 94 Zero Solvent Counter 94 Pressure Above Upper Limit 95 Pressure Below Lower Limit 96 Pressure Signal Missing 97 Valve Failed 98 Missing Pressure Reading 99 Pump Configuration 99 Valve Fuse 100 Inlet Valve Fuse 101 Temperature Out of Range 101 Temperature Limit Exceeded 102 Motor Drive Power 103 Encoder Missing 104 fee Agilent Technologies 85 7 86 Error Information Inlet Valve Missing 105 Servo Restart Failed 106 Pump Head Missing 107 Index Limit 108 Index Adjustment 109 Index Missing 110 Stroke Length 111 Initialization Failed 112 Wait Timeout 113 Electronic fuse of SSV 114 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 1260 Binary Pump VL User Manual Error Information 7 What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook 1260 Binary Pump VL User Manual 87 7 Error Information General Error Messages 88 General error messages are
79. hyl acetate 104 Heptane 120 Hexane 150 Isobutanol 100 lsopropanol 100 Methanol 120 1 Propanol 100 Toluene 87 Water 46 6 Repeat step 1 on page 76 through step 5 on page 76 for the B channel of your binary pump 1260 Binary Pump VL User Manual 77 5 Optimizing Performance How to Optimize the Compressibility Compensation Setting 78 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 6 Troubleshooting and Diagnostics Status Indicators 80 Status Indicators 81 Power Supply Indicator 81 Module Status Indicator 82 User Interfaces 83 Agilent Lab Advisor Software 84 Overview about the troubleshooting and diagnostic features ee Agilent Technologies 79 6 Troubleshooting and Diagnostics Status Indicators 80 The module is provided with two status indicators which indicate the operational state prerun run and error states of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components see Test
80. iagnostics Agilent Lab Advisor Software 84 The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files This manual provides lists with the names of Error Messages Not Ready messages and other common issues 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 7 Error Information What Are Error Messages 87 General Error Messages 88 Timeout 88 Shut Down 88 Remote Timeout 89 Synchronization Lost 89 Leak Sensor Short 90 Leak Sensor Open 90 Compensation Sensor Open 91 Compensation Sensor S
81. ical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch The power input socket accepts a line voltage of 100 240 VAC 10 witha line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1260 Binary Pump VL User Manual Introduction 1 Electrical Connections Serial Number Information ALL Configuration switch GPIB Slot for interface board RS232 Remote Analog output CAN Bus Power Plug Security Lever The serial number information on the instrument labels provide the following information CCXZZ00000 Format CC Country of manufacturing DE Germany X Alphabetic character A Z used by manufacturing ZZ Alpha numeric code 0 9 A Z where each combination unambiguously denotes a module there can be more than one code for the same module 00000 Serial number
82. iled The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated This limit is given by 2 revolutions second for longer than 5 seconds Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 4 Improperly positioned cables or wires Ensure the fan is not mechanically blocked obstructing fan blades A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 3 Loose or leaking purge valve inlet valve or Ensure pump components are seated correctly outlet valve If there are still signs of a leak exchange the appropriate seal purge valve inlet valve outlet valve 4 Defective pump seals Exchange the pump seals 1260 Binary Pump VL User Manual
83. ilent com In This Book This manual contains technical reference information about the Agilent 1260 Infinity Binary Pump VL G1312C The manual describes the following introduction requirements e installation configuring the pump e using the pump e optimizing performance troubleshooting and diagnostics e maintenance e parts and materials identifying cables e safety Agilent Technologies 2008 2010 Printed in Germany 06 10 G1312 90007 EE Agilent Technologies
84. imits the error message is generated Probable cause Suggested actions 1 Solvent selection valve disconnected Ensure the solvent selection valve is connected correctly 2 Connection cable inside instrument not Please contact your Agilent service connected representative 3 Connection cable inside instrument Please contact your Agilent service defective representative 4 Solvent selection valve defective Exchange the solvent selection valve 98 1260 Binary Pump VL User Manual Error Information 7 Missing Pressure Reading The pressure readings read by the pump ADC analog digital converter are missing The ADC reads the pressure signal of from the damper every Ims If the readings are missing for longer than 10 seconds the error message is generated Probable cause Suggested actions 1 Damper disconnected Please contact your Agilent service representative 2 Defective damper Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Pump Configuration At switch on the pump has recognized a new pump configuration The pump is assigned its configuration at the factory If the active inlet valve and pump encoder of channel B are disconnected and the pump is rebooted the error message is generated However the pump will function as an isocratic pump in this configuration The error message reappears after each switch on Probable cause Suggest
85. ing 1mm I D 3 m p n 0890 1764 Seal 2 packs of 2 for binary pump p n 5063 6589 Seals insert tool p n 01018 2370 1260 Binary Pump VL User Manual 183 10 Parts for Maintenance Solvent Cabinet Solvent Cabinet Item p n Description 1 5065 9981 Solvent cabinet including all plastic parts 2 5043 0207 Name plate 1260 4 5042 8567 Leak pan 5 9301 1420 Solvent bottle transparent 6 9301 1450 Solvent bottle amber 7 G1311 60003 Bottle head assembly CS pm Figure 34 Solvent Cabinet Parts 184 1260 Binary Pump VL User Manual Bottle Head Assembly Item p n 1 9301 1450 2 9301 1420 3 G1311 60003 4 5063 6598 5 5063 6599 6 5062 2483 7 5062 8517 8 5041 2168 Parts for Maintenance 10 Description Solvent bottle amber Solvent bottle transparent Bottle head assembly Ferrules with lock ring 10x Tube screw 10x Solvent tubing 5 m Inlet filter adapter pack of 4 Solvent inlet filter 20 pm NS 4 fl a S 8 y Figure 35 Bottle Head Assembly Parts 1260 Binary Pump VL User Manual 185 10 Parts for Maintenance Hydraulic Path with Solvent Selection Valve 186 Table 23 Hydraulic Path with Solvent Selection Valve Item Description Part Number 1 Capillary mixer to purge valve p n G1312 67308 2 Capillary outlet ball valve to piston 2 p n G1312 67300 3 Mixer p n G1312 87330 4 Bottle head assembly p n G1311 60003 5 Capillary damper to mixer p n G1312 67307 6 Restriction capillary p n G1312 673
86. instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 32 1260 Binary Pump VL User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cor
87. ion oa Ff amp w N Repeat step 1 on page 62 through step 4 on page 62 for the other channel s of the binary pump 6 Close the purge valve and set the required composition and flow rate for your application Table 16 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of the system When switching between lsopropanol Best solvent to flush air out of reverse phase and normal the system phase both times After an installation Ethanol or Methanol Alternative to lsopropanol second choice if no Isopropanol is available To clean the system when Bidistilled water Best solvent to re dissolve using buffers buffer cristals After a solvent change Bidistilled water Best solvent to re dissolve buffer cristals After the installation of normal Hexane 5 lsopropanol Good wetting properties phase seals P N 0905 1420 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 4 Using the Binary Pump Hints for Successful Use of the Binary Pump 64 Solvent Information 66 Prevent Blocking of Solvent Filters 67 Algae Growth in HPLC Systems 68 How to Prevent and or Reduce the Algae Problem 69 This chapter provides information for optimized usage of the binary pump gy Agilent Technologies 63 4 Using the Binary Pump Hints for Successful Use of the Binary Pump 64 Place solvent cabinet with the solvent bottles
88. ion Item p n 1 5065 9953 5042 8507 2 5067 4695 3 G1312 60062 4 G4220 63010 5 0905 1175 6 01018 07102 7 G4220 26210 8 G4220 24013 9 G1312 67300 10 0905 1503 11 0515 0175 12 G1312 23200 13 G1312 60061 14 G1312 60067 15 5042 1303 16 G1312 25260 17 G1312 60066 18 G1312 23201 19 0515 2118 1260 Binary Pump VL User Manual Description Seal wash pump assembly Seal wash pump cartridge Sapphire piston default Piston housing incl spring Support Ring Seal Wash Wash seal Gasket Seal wash Seal Holder Backup Ring for Seal Holder Capillary outlet valve to piston 2 Piston seal PTFE carbon filled black pack of 2 default Mounting screw for manual purge valve holder M4 20 mm long Holder for manual purge valve Purge valve assembly Outlet valve complete Screw lock Pump housing Passive inlet valve Adapter Screw M5 60 mm long 177 10 Parts for Maintenance Pump Head Assembly with Seal Wash Option Figure 31 Pump Head Assembly With Seal Wash Option 178 1260 Binary Pump VL User Manual Parts for Maintenance 10 Outlet Valve Outlet Valve p n Description G1312 60067 Outlet valve complete Figure 32 Outlet Valve 1260 Binary Pump VL User Manual 179 10 Parts for Maintenance Purge Valve Assembly Purge Valve Assembly Item p n Description 1 G1312 60061 Purge valve assembly 2 01018 22707 PTFE frit pack of 5 3 5067 4728 Seal cap 180 1260 Binary Pump VL
89. ion 32 power consumption 35 power cords 33 precision 64 pressure above upper limit 95 pressure below lower limit 96 pressure sensor readings 52 pressure test 116 pressure pulsation 13 36 76 pressure range 74 pressure operating range 36 priming withapump 60 62 with a syringe 60 with a pump 72 with a syringe 72 PTFE frit 133 pump head missing 107 pump seals 143 pump seals 64 133 purge valve 64 133 purge valve assembly 11 purging the pump 60 radio interference 211 reassembling the pump head 149 recommended pH range 36 remote cable 196 removing the pump head 140 repair procedures 133 repairs replacing firmware 170 170 reproducibility 64 restart without cover 94 results pressure test 119 RS 232C cable 203 communication settings 27 running the leak test 123 running the pressure test 118 217 Index S safety class 206 safety information lithium batteries 210 safety general information 206 standards 35 symbols 208 sapphire piston 10 screwdriver pozidriv 1 140 screwdriver pozidriv 1 147 157 screwdriver flat head 147 sealwash 73 sealwash 10 whentouse 73 seal wear counter 144 seal alternative material 74 seals 64 133 147 160 160 security lever 49 selection valve failed 97 selection valve fuse 100 serial number information 17 servo restart failed 106 setable flowrange 36 shut down 88 sieve 133 153 simple repair procedures 133 simple repairs 130 site requirements 31 power cords 33 snap
90. llow indicator indicates a not ready condition The module is ina not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running e An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis A red blinking modules with on board LAN or yellow blinking modules without on board LAN indicator indicates that the module is in resident mode e g during update of main firmware A fast red blinking modules with on board LAN or fast yellow blinking modules without on board LAN indicator indicates that the module is in boot loader mode e g during update of main firmware In such a case try to re boot the module or try a cold start 82 1260 Binary Pump VL User Manual Troubleshooting and Diagnostics 6 User Interfaces Depending on the user interface the available tests vary Some descriptions are only available in the Service Manual Table 18 Test Functions available vs User Interface Test ChemStation Instant Pilot G4208A Agilent Lab Advisor Pressure Test Yes No Leak Test Yes No 1260 Binary Pump VL User Manual 83 Troubleshooting and D
91. local Agilent Technologies sales and service office Table 15 Binary Pump Checklist Description Quantity Binary Pump 1 Solvent cabinet p n 1 5062 8591 1260 Binary Pump VL User Manual Installing the Pump 3 Table 15 Binary Pump Checklist Description Quantity Solvent bottle amber p n 2 g 4 9301 1450 Solvent bottle transparent p n 9301 1420 Bottle head assembly p n 2 or 4 G1311 60003 Waste tube purge valve 1 5m p n 5042 2461 Power cord 1 CAN cable Agilent module 1 to module 1 m p n 5181 1519 Remote cable As ordered Signal cable As ordered Service Manual 1 Accessory Kit p n 1 G1312 68765 1 ifthe binary pump is equipped with a solvent selection valve 1260 Binary Pump VL User Manual 41 3 42 Installing the Pump Accessory Kit Accessory Kit p n G1312 68755 p n 0890 1195 5063 6527 5181 1519 5988 8453EN 9222 0519 G1312 87303 G1312 87304 01200 60001 G1311 90107 5042 9954 4x G1311 60003 2x Description Drain tube Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste CAN cable Agilent module to module 1 m Capillary fitting starter kit brochure Bag plastic SS Capillary 400 x 0 17 mm m m ps ps SST capillary 700 mm 0 17 mm i d 1 32 1 32 RRLC system configurator A 01 01 CD ROM Algae note Tubing clip 2x re order 4 pk Bottle head assembly 1260 Binary Pump VL User Manual Installing the Pump 3 Optimizing the Stack C
92. low of solvent into the unused pump channel Whenever exchanging the pump seals the purge valve frit and the outlet ball valve sieve should be exchanged too When using buffer solutions flush the system with water before switching it off The seal wash option should be used when buffer solutions of 0 1 M or higher will be used for long time periods 1260 Binary Pump VL User Manual Using the Binary Pump 4 e Check the pump pistons for scratches when changing the piston seals Scratched pistons will lead to micro leaks and will decrease the lifetime of the seal e After changing the piston seals apply the seal wear in procedure see Exchanging the Pump Seals on page 143 e Place the aqueous solvent on channel A and the organic solvent on channel B The default compressibility settings are set accordingly 1260 Binary Pump VL User Manual 65 4 Using the Binary Pump Solvent Information Always filter solvents through 0 4 um filters small particles can permanently block the capillaries and valves Avoid the use of the following steel corrosive solvents 66 Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric and nitric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halo
93. lve cartridge it may take several ml of pumping with the solvent used in the current application before the flow stabilizes at a ripple as low as it used to be when the system was still working properly 1260 Binary Pump VL User Manual 167 9 Maintenance Exchanging the Optional Interface Board When Parts required CAUTION Board defective Description 1 BCD Interface board Electronic boards are static sensitive and should be handled with care so as not to damage them Touching electronic boards and components can cause electrostatic discharge ESD ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap when handling electronic boards and components 168 1 Switch OFF the module at the main power switch Unplug the module from main power 2 Disconnect cables from the interface board connectors 3 Loosen the screws Slide out the interface board from the module Install the new interface board Secure the screws 1260 Binary Pump VL User Manual Maintenance 9 Exchanging the Optional Interface Board 5 Reconnect the cables to the board connector BCD Interface board Figure 27 Exchanging the Interface Board 1260 Binary Pump VL User Manual 169 9 Maintenance Replacing the Module s Firmware When Tools required Parts required Preparations
94. lves The figures below show a Binary Pump G1312B The kit can be used similarly for the Binary Pump G1312C Figures show passive inlet valves If the pump uses active inlet valves they can be used similarly 1 Remove the front cover sheet by removing both screws 2 Plug in the connector of the solvent selection valve Connector SSV cable Solvent selection valve 1260 Binary Pump VL User Manual 155 Maintenance Installat oft the 3 156 Install the Solvent selection assembly by fixing both indicated screws 4 Connect the outlet for solvent A upper row to the inlet valve of the left pump head Connect the outlet for solvent B lower row to the inlet valve of the right pump head Put the solvent bottles into the solvent cabinet Connect the bottle heads of solvents A1 and A2 to the inlets in the upper row see labels on valve assembly Connect the bottle heads of solvents B1 and B2 to the inlets in the lower row see labels on valve assembly To solvent bottles Inlets Outlets For controlling the solvent selection valve please refer to the online help or user manual of your control software 1260 Binary Pump VL User Manual Maintenance 9 Exchanging the Solvent Selection Valve When If leaking internally croossflow between the ports or if one of the channels is blocked Tools required Screwdriver Pozidriv 1 P
95. mp head screws in channel A Ensure the pump head screws in channel A are tight 4 Loose outlet valve in channel A Ensure the sieve in the outlet valve is installed correctly Tighten the outlet valve 5 Leaking seal or scratched piston in channel Exchange the pump seals in channel A Check Al the pistons for scratches Exchange if scratched 6 Defective active inlet valve in channel A Exchange the active inlet valve in channel A 1260 Binary Pump VL User Manual 127 8 Test Functions and Calibration Fourth plateau negative or unstable and at least one other plateau positive Probable cause Suggested actions 1 Airin pump chamber of channel B or seals Flush channel B thoroughly with isopropanol not yet seated under pressure restriction capillary 2 Loose active inlet valve in channel B Tighten the active inlet valve in channel B 14mm wrench Do not overtighten 3 Loose pump head screws in channel B Ensure the pump head screws in channel B are tight 4 Loose outlet valve in channel B Ensure the sieve in the outlet valve is installed correctly Tighten the outlet valve 5 Leaking seal or scratched piston in channel Exchange the pump seals in channel B Check B1 the pistons for scratches Exchange if scratched 6 Defective active inlet valve in channel B Exchange the active inlet valve in channel B 128 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 9 Maintenance Introduction to Maintenance an
96. n agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage
97. n the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for e 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable 1260 Binary Pump VL User Manual Introduction 1 The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table3 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 o 2 RX TX 3 X 3 TX DTR 4 E 4 DTR GND 5 X 5 GND DSR 6 6 DSR RTS 7 X 7 RTS CTS 8 1 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 5 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board 1260 Binary Pump VL User Manual 21 1 22 Introduction APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to u
98. nd the connecting tube from solvent selection valve if installed to the AIV 7 Rinse your system with the solvent used for your next application 1260 Binary Pump VL User Manual 145 9 Maintenance Exchanging the Plungers When When scratched Tools required 3 mm hexagonal key 4 mm hexagonal key Parts required p n Description 1 5063 6586 Piston Preparations Switch off binary pump at power switch Remove the front cover to have access to the pump mechanics Removing the Pump Head Assembly on page 140 1 Check the piston surface and remove any deposits or layers Cleaning can be done with alcohol or tooth paste Replace piston if scratched A PAN Piston surface 2 Reassemble the pump head assembly see Reinstalling the Pump Head Assembly on page 149 146 1260 Binary Pump VL User Manual Exchanging the Wash Seals When If leaking Tools required hexagonal key3 mm 4 mm hexagonal key Insert tool Small flat head screwdriver Parts required p n Description 0905 1175 Wash seal Maintenance 6 5062 2484 Gasket seal wash pack of 6 Preparations Assembly on page 140 Switch off binary pump at power switch Remove the front cover to have access to the pump mechanics Removing the Pump Head 9 1 Remove the seal keeper and the seal wash support rings from the plunger housing Remove the seal keeper from the support ring assembly
99. ng the Pump Seals 3 Using the insert tool insert the new seals into the pump head and press them firmly in position Insert tool Pump seals 4 Reassemble the pump head assembly see Reinstalling the Pump Head Assembly on page 149 NOTE Reset the seal wear counter and liquimeter in the Agilent Lab Advisor 144 1260 Binary Pump VL User Manual Maintenance 9 Seal Wear in Procedure This procedure is required for standard seals only 5063 6589 but it will definitely damage the normal phase application seals 0905 1420 1 Place a bottle with 100 ml of isopropanol in the solvent cabinet and place the tubing including bottle head assembly of the pump head that is supposed to be worn in into the bottle 2 Screw the adapter 0100 1847 to the AIV and connect the inlet tube from the bottle head directly to it 3 Connect the restriction capillary 5022 2159 to the purge valve Insert its other end into a waste container 4 Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 mL min 5 Close the purge valve set the flow to a rate adequate to achieve a pressure of 350 bar Pump 15 min at this pressure to wear in the seals The pressure can be monitored using your instrument control software or tool 6 Turn OFF the pump slowly open the purge valve to release the pressure from the system disconnect the restriction capillary and reconnect the outlet capillary at the purge valve a
100. ns Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative 1260 Binary Pump VL User Manual Error Information 7 Compensation Sensor Open The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Compensation Sensor Short The ambient compensation sensor NTC on the main board in the module has failed short circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative 1260 Binary Pump VL User Manual 91 7 92 Error Information Fan Failed Leak The cooling fan in the module has fa
101. nsure solvent inlet filters are not blocked 3 Leak Inspect the pump head capillaries and fittings for signs of a leak Purge the module Run a pressure test to determine whether the seals or other module components are defective 4 Defective damper Please contact your Agilent service representative 5 Defective main board Please contact your Agilent service representative 96 1260 Binary Pump VL User Manual Error Information 7 Pressure Signal Missing The pressure signal of the damper is missing The pressure signal of the damper must be within a specific voltage range If the pressure signal is missing the processor detects a voltage of approximately 120mV across the damper connector Probable cause Suggested actions 1 Damper disconnected Please contact your Agilent service representative 2 Defective damper Please contact your Agilent service representative 1260 Binary Pump VL User Manual 97 7 Error Information Valve Failed Following errors can only occur if a solvent selection valve is used The internal number in the error message is linked to one of the valves as shown in the following table Valve 0 Failed valve A1 Valve 1 Failed valve A2 Valve 2 Failed valve B2 Valve 3 Failed valve B1 One of the solvent selection valves in the binary pump failed to switch correctly The processor monitors the valve voltage before and after each switching cycle If the voltages are outside expected l
102. ommended when e vacuum degasser is used for the first time or vacuum tubes are empty or e changing to solvents that are immiscible with the solvent currently in the vacuum tubes Priming the vacuum degasser by using the pump at high flow rate 3 5 mL min is recommended when pump was turned off for a length of time for example during night and volatile solvent mixtures are used or solvents have been changed For more information see the Agilent 1260 Infinity Standard Degasser User Manual p n G1322 90012 1260 Binary Pump VL User Manual Optimizing Performance 5 When to Use the Seal Wash Option active seal wash Highly concentrated buffer solutions will reduce the lifetime of the seals and pistons in your pump The seal wash option allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent The seal wash option is strongly recommended when buffer concentrations of 0 1 M or higher will be used for long time periods in the pump The active seal wash upgrade can be ordered as G1398A The seal wash option comprises a support ring secondary seal gasket and seal holder for both piston sides A wash bottle filled with water isopropanol 90 10 is placed above the pump in the solvent cabinet and the peristaltic pump moves a flow through the pump head removing all possible buffer crystals from the back of the pump seal Running dry is the worst case for a wash seal and drastically redu
103. onfiguration If your module is part of a complete Agilent 1260 Infinity Liquid Chromatograph you can ensure optimum performance by installing the following configurations These configurations optimize the system flow path ensuring minimum delay volume One Stack Configuration Ensure optimum performance by installing the modules of the Agilent 1260 Infinity LC System in the following configuration See Figure 8 on page 44 and Figure 9 on page 45 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required 1260 Binary Pump VL User Manual 43 3 Installing the Pump Optimizing the Stack Configuration Solvent cabinet Vacuum degasser Local User Interface Autosampler Column compartment Detector Figure 8 Recommended Stack Configuration for 1260 Front View 1260 Binary Pump VL User Manual Installing the Pump 3 Optimizing the Stack Configuration Remote cable CAN Bus cable to local user interface AC power CAN Bus cable Analog detector signal 1 or 2 outputs per detector LAN to LC ChemStation location depends on detector Figure 9 Recommended Stack Configuration for 1260 Rear View 1260 Binary Pump VL User Manual 45 3 Installing the Pump Two Stack Configuration To avoid excessive height of the stack when the autosampler thermostat is added to the system it is recommended to form two stacks Some
104. onnector of the pump see Figure 9 on page 45 5 Press in the power switch to turn on the vacuum degasser The AUX output is intended for troubleshooting It provides a DC voltage in the range of 0 1 V which is proportional to the vacuum level in the degasser chambers 52 1260 Binary Pump VL User Manual Installing the Pump 3 Connecting Control Software and or G4208 A Instant Pilot With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN Usually the detector is producing the most data in the stack followed by the pump and it is therefore highly recommended to use either of these modules for the LAN connection 1 Ensure the power switches on the front of the modules in the stack are OFF switches stand out 2 If there are no other 1260 with LAN port in the HPLC stack install a G1369B LAN board into the extension slot of the pump 3 Connect the LAN enabled module with a LAN cable to the data system 4 Plug the CAN connector of the Instant Pilot into any available CAN port of the 1260 system 5 Plug a CAN cable into the CAN connector of the Instant Pilot The Standard Degasser must not be connected to LAN or CAN as its connector is for diagnostic use only 6 Connect the CAN cable to the CAN connector of one of the modules 7 Press in the power switches to turn on the modules The Agilent control software can also be connected to the system through
105. orking with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The amount of substances should be reduced to the minimal volume required for the analysis gt Do not operate the instrument in an explosive atmosphere Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the metal top cover of the module No serviceable parts inside gt Only certified persons are authorized to carry out repairs inside the module Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 1260 Binary Pump VL Us
106. ower 47 external contact 202 interface 50 LAN 201 remote 196 RS 232 203 cables analog 192 BCD 192 CAN 193 external contact 193 LAN 193 overview 192 remote 192 RS 232 193 CAN cable 201 changing solvents 60 checklist binary pump 40 cleaning 134 column 8 Communication settings RS 232C 27 communications 37 compensation sensor open 91 compensation sensor short 91 composition precision 37 37 composition range 37 compressibility compensation 13 36 76 condensation 34 configuration onestack 43 43 two stack 46 connections flow 54 57 control software 53 D damaged parts 40 damping unit 11 defect on arrival 40 degreaser spray 147 delay volume 37 75 delay volume 12 215 Index delivery checklist 40 design 9 Diagnostic software 84 dimensions 35 dual piston in series design 9 E electrical connections descriptions of 16 electronic waste 209 electrostatic discharge ESD 168 EMF early maintenance feedback 14 encoder missing 104 error messages fan failed 92 error messages compensation sensor open 91 compensation sensor short 91 encoder missing 104 ignition without cover 93 93 index adjustment 109 index limit 108 index missing 110 initialization failed 112 inlet valve fuse 101 inlet valve missing 105 eak sensor open 90 eak sensor short 90 eak 92 missing pressure reading 99 motor drive power 103 pressure above upper limit 95 pressure below lower limit 96 pump head missing 107 remo
107. peat the pressure test Exclude each module one by one to determine which module is leaking Ifthe pump is determined to be the source of the leak run the leak test 1260 Binary Pump VL User Manual 119 120 Test Functions and Calibration System Pressure Test failed The test will fail if the sum of all leaks in the system pump autosampler or column compartment and connections exceeds the test limit After isolating and fixing the cause of the leak repeat the System Pressure Test to confirm the system is pressure tight Probable cause 1 Purge valve open 2 Loose or leaky fittings 3 Pump Damaged pump seals or pistons 4 Loose purge valve 5 Autosampler Loose or leaky fitting 6 Autosampler Rotor seal injection valve 7 Autosampler Damaged metering seal or piston 8 Autosampler Needle seat 9 Column compartment Loose or leaky fitting 10 Column compartment Rotor seal column switching valve Suggested actions Close the purge valve Tighten the fitting or exchange the capillary Run the Leak Rate Test to confirm the leak Tighten the purge valve nut 14 mm wrench Tighten or exchange the fitting or capillary Exchange the rotor seal Exchange the metering seal Check the piston for scratches Exchange the piston if required Exchange the needle seat Tighten or exchange the fitting or capillary Exchange the rotor seal 1260 Binary Pump VL User Manual Test Functions and Calibration
108. ped with default configuration settings To change these settings see Setting the 8 bit Configuration Switch without On Board LAN on page 26 50 1260 Binary Pump VL User Manual Installing the Pump 3 Connecting Modules and Control Software WARNING Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Connecting Agilent 1260 Infinity Modules 1 Place the individual modules in a stack configuration as shown in Figure 8 on page 44 2 Ensure the power switches on the front of the modules are OFF switches stand out 3 Plug a CAN cable into the CAN connector at the rear of the respective module except vacuum degasser 4 Connect the CAN cable to the CAN connector of the next module see Figure 9 on page 45 5 Press in the power switches to turn on the modules 1260 Binary Pump VL User Manual 51 3 Installing the Pump Connecting an Agilent 1260 Infinity Vacuum Degasser 1 Place the vacuum degasser in the stack of modules as shown in Figure 8 on page 44 2 Ensure the power switch at the front of the vacuum degasser is OFF switch stands out 3 Plug an APG cable into the APG remote connector at the rear of the degasser 4 Connect the APG cable to the APG remote c
109. point connection Twisted pair network cable shielded 7 m for point to point connection 201 11 Identifying Cables External Contact Cable 5o C G 1 100 lt 6 ie e e11 One end of this cable provides a 15 pin plug to be connected to Agilent modules interface board The other end is for general purpose Agilent Module Interface Board to general purposes p n G1103 61611 Color Pin Agilent Signal Name module White 1 EXT 1 a Brown 2 EXT 1 x Green 3 EXT 2 Bea Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 202 1260 Binary Pump VL User Manual Identifying Cables 11 Agilent Module to PC p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m 1260 Binary Pump VL User Manual 203 11 Identifying Cables Agilent 1200 Module to Printer p n Description 5181 1529 Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT F
110. positioned at the column compartment outlet or the outlet of the last module before the detector If a specific component is suspected of causing a system leak place the blank nut immediately before the suspected component then run the Pressure Test again If the test passes the defective component is located after the blank nut Confirm the diagnosis by placing the blank nut immediately after the suspected component The diagnosis is confirmed if the test fails 1260 Binary Pump VL User Manual 117 8 Test Functions and Calibration Running the Pressure Test When Tools required Parts required Preparations 118 If problems with small leaks are suspected After maintenance of flow path components e g pump seals injection seal to prove pressure tightness up to 400 bar Wrench 1 4 inch p n Description 1 01080 83202 Blank nut 1 500 ml lsopropanol Place a bottle of LC grade isopropanol in the solvent cabinet and connect it to channel A or channel A2 if solvent selection valve is installed Make absolutely sure that all parts of the flow path that are part of the test are flushed very thoroughly with isopropanol before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail Running the test from the Agilent Lab Monitor amp Diagnostic Software 1 Select the pressure test from the test selection menu 2 Start the test and
111. ptional Interface Board on page 168 If solvent filter is blocked If internally leaking If internally leaking If internally leaking If the frit shows indication of contamination or blockage If internally leaking If pump performance indicates seal wear If scratched When seals show indication of leaks If defective Gradient performance problems intermittent pressure fluctuations Pressure ripple unstable run leak test for verification Pressure ripple unstable run leak test for verification Solvent dripping out of waste outlet when valve closed A pressure drop of gt 10 bar across the frit 5 ml min H20 with purge valve open indicates blockage Error messages Valve failed or Valve Fuse are generated Leaks at lower pump head side unstable retention times pressure ripple unstable run leak test for verification Seal life time shorter than normally expected check plungers while changing the seals Leaks at lower pump head side loss of wash solvent Error condition indicated by red status indicator 1260 Binary Pump VL User Manual 133 9 Maintenance Cleaning the Module The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth as liquid may drip into the module WARNING Liquid dripping into the electronic compartment of your mo
112. r index position is reached If the time to reach the index position is too long the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Please contact your Agilent service representative 1260 Binary Pump VL User Manual 109 7 Error Information Index Missing Index Missing Pump channel A B Index Missing Pump channel B The encoder index position in the module was not found during initialization During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is not recognized within a defined time the error message is generated Probable cause Suggested actions 1 Disconnected or defective encoder cable Please contact your Agilent service representative 2 Defective pump drive assembly Please contact your Agilent service representative 110 1260 Binary Pump VL User Manual Error Information 7 Stroke Length Stroke Length Pump channel A B Stroke Length Pump channel B The distance between the lower piston position and the upper mechanical stop is out of limits pump During initialization the module monitors the drive current If the pi
113. r Manual Maintenance 9 Exchanging the Inlet Valve When If internally or externally leaking Tools required Wrench 14 mm Parts required Description G1312 60066 Passive inlet valve Preparations Switch off the pump at the main power switch 1 Remove the front cover 2 Disconnect the solvent inlet tube at the inlet valve beware of leaking solvents 1260 Binary Pump VL User Manual 151 9 Maintenance Exchanging the Inlet Valve 3 Using a 14 mm wrench loosen the inlet valve and remove the valve from the pump head 4 Screw the valve into the pump head Using the 14 mm wrench turn the nut until it is hand tight 5 Using the 14 mm wrench tighten the valve do not over tighten 6 Reinstall the front cover NOTE After an exchange of the valve it may be required to pump several mL of the solvent used in the current application before the flow stabilizes at a pressure ripple as low as it used to be when the system was still working properly 152 1260 Binary Pump VL User Manual Maintenance 9 Exchanging the Outlet Valve When Tools required Parts required Preparations if leaking internally tools wrench 1 4 5 16 inchWrench 1 4 inch Wrench 14 mm Description G1312 60067 Outlet valve complete Switch off the pump at the main power switch Before exchanging the outlet valve you can try to clean it in an ultrasonic bath Remove the gold seal and put the plastic cap back on to protect the sealin
114. rating 4 55 C 41 131 F temperature Ambient non operating 40 70 C 4 158 F temperature Humidity lt 95 at 25 40 C 77 104 F Non condensing Operating Altitude Up to 2000 m 6562 ft Non operating altitude Up to 4600 m 15091 ft For storing the module Safety standards IEC CSA Installation Category II Pollution Degree 2 For indoor use only UL 1260 Binary Pump VL User Manual 35 2 Site Requirements and Specifications Performance Specifications 36 Table 14 Performance Specification Agilent 1260 Infinity Binary Pump VL G1312C Type Hydraulic system Setable flow range Flow range Flow precision Flow accuracy Pressure Pressure pulsation Compressibility compensation Recommended pH range Gradient formation Specification Dual piston in series pump with proprietary servo controlled variable stroke drive floating pistons Setpoints 0 001 5 mL min in 0 001 mL min increments 0 1 5 0 mL min lt 0 07 RSD or lt 0 02 min SD whatever is greater based on retention time at constant room temperature 1 or 10 pL min whatever is greater pumping degassed H20 at 10 MPa Operating range 0 40 MPa 0 400 bar 0 5880 psi up to 5 mL min lt 2 amplitude typically lt 1 3 or lt 3 bar at 1 mL min isopropanol at all pressures gt 10 bar 147 psi User selectable based on mobile phase compressibility 1 0 12 5 solvents with
115. rs Al A2 B1 and B2 of the solvent selection valve and label the tubes accordingly Fix the tubes in the clips of solvent cabinet and binary pump 5 Using a piece of sanding paper connect the waste tubing to the purge valve and place it into your waste system 6 Ifthe binary pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack connect the corrugated waste tube to the waste outlet of the pump leak handling system 7 Connect the outlet capillary binary pump to injection device to the outlet of the purge valve 1260 Binary Pump VL User Manual 55 3 Installing the Pump Flow Connections of the Binary Pump with Solvent Selection Valve 8 Purge your system before first use see Priming and Purging the System on page 60 Bottle head assembly il i l L Solvent cabinet Po mH E Tube clip Tm ee on aa P EE Masam on a tine Sato Mirer fra k s H Id Channel A1 LOL CNP O Channel B1 amn aN i Purge valve Tojar 1 SH mT neee E Pump headchannel A E 5 K ee Aja S e el SZ a a Active inlet valve A ETE Pes Ly Active inlet 2 a er oe Waste tubing A Waste outlet Outlet capillary to autosampler Channel A2 Channel B2 Solvent selection valve Figure 15 Binary Pump with Solvent Selection Valve 56 1260 Binary Pump VL User Manual Installing the Pump 3 Flow Connections of the Binary Pump Without Solvent S
116. s Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1260 Binary Pump VL User Manual 207 12 Appendix Safety Symbols Table 25 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the A instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced G by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up WARNING iia alerts you to situations that could cause physical injury or death
117. s and Calibrations System Pressure Test The System Pressure Test is a quick test designed to determine the pressure tightness of the system After exchanging flow path components e g pump seals or injection seal use this test to verify the system is pressure tight Leak Rate Test The Leak Rate Test is a diagnostic test designed to determine the pressure tightness of the pump When a problem with the pump is suspected use this test to help troubleshoot the pump and its pumping performance 1260 Binary Pump VL User Manual Troubleshooting and Diagnostics 6 atus indicators Status Indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status Status Indicator Power switch Serial number Figure 18 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON 1260 Binary Pump VL User Manual 81 6 Troubleshooting and Diagnostics Module Status Indicator The module status indicator indicates one of six possible module conditions e When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis e A green status indicator indicates the module is performing an analysis run mode A ye
118. s for RS 232C 27 Special Settings 29 This chapter gives an introduction to the module instrument overview and internal connectors ee Agilent Technologies 1 Introduction Introduction to the Binary Pump Introduction to the Binary Pump The binary pump comprises two identical pumps integrated into one housing It provides gradient generation byhigh pressure mixing Degassing is not included but a vacuum degasser is available as a separate product for applications that require best flow stability especially at low flow rates or maximum detector sensitivity This is most likely required to run small internal diameter columns 2 mm and 1 mm i d which require low flow rates A solvent selection valve optional will allow to select a binary mixture isocratic and gradient from four independent solvent bottles An active seal wash optional is available when the pump is used with concentrated buffer solutions Power supply HPM board Pump drive Mixer Damper Outlet valve Pump head A Purge valve Mixing chamber Solvent selection valve amp Pump head B Active inlet valve Leak sensor Figure 1 Overview of the Binary Pump 8 1260 Binary Pump VL User Manual Introduction 1 Overview of the Binary Pump The binary pump is based on a two channel dual piston in series design which comprises all essential functions that a solvent delivery system has to fulfill Metering of solvent and delivery to the hi
119. s mobile phase 1260 Binary Pump VL User Manual 69 4 Using the Binary Pump Algae Growth in HPLC Systems 70 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 5 Optimizing Performance When to Use a Vacuum Degasser 72 When to Use the Seal Wash Option active seal wash 73 Choosing the Right Pump Seals 74 When to Remove the Static Mixer 75 How to Optimize the Compressibility Compensation Setting 76 This chapter gives hints on how to optimize the performance or use additional devices te Agilent Technologies n 5 Optimizing Performance When to Use a Vacuum Degasser 72 The pump does not necessarily require degassing But for the following conditions the vacuum degasser is recommended e if your detector is used with maximum sensitivity in the low UV wavelength range e if your application requires highest injection precision or e if your application requires highest retention time reproducibility mandatory at flow rates below 0 5 mL min Operational Hints for the Vacuum Degasser If you are using the vacuum degasser for the first time if the vacuum degasser was switched off for any length of time for example overnight or if the vacuum degasser lines are empty you should prime the vacuum degasser before running an analysis The vacuum degasser can be primed either by drawing solvent through the degasser with a syringe or by pumping with the pump Priming the degasser with a syringe is rec
120. se features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal 1260 Binary Pump VL User Manual Introduction 1 Table4 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L R
121. ston reaches the upper mechanical stop position before expected the motor current increases as the module attempts to drive the piston beyond the mechanical stop This current increase causes the error message to be generated Probable cause Suggested actions 1 Defective pump drive assembly Please contact your Agilent service representative 1260 Binary Pump VL User Manual 111 7 Error Information Initialization Failed Initialization Failed Pump channel A B Initialization Failed Pump channel B The module failed to initialize successfully within the maximum time window A maximum time is assigned for the complete pump initialization cycle If the time is exceeded before initialization is complete the error message is generated Probable cause Suggested actions 1 Blocked passive inlet valve Exchange the inlet valve 2 Defective pump drive assembly Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 112 1260 Binary Pump VL User Manual Error Information 7 Wait Timeout When running certain tests in the diagnostics mode or other special applications the pump must wait for the pistons to reach a specific position or must wait for a certain pressure or flow to be reached Each action or state must be completed within the timeout period otherwise the error message is generated Possible Reasons for a Wait Timeout Pressure not reached
122. t Configuration Switch without On Board LAN The 8 bit configuration switch is located at the rear of the module Modules that do not have their own LAN interface e g the TCC can be controlled through the LAN interface of another module and a CAN connection to that module Figure 7 Configuration switch settings depend on configured mode All modules without on board LAN e default is ALL DIPS DOWN best settings Bootp mode for LAN e for boot test modes DIPS 1 2 must be UP plus required mode Switch settings provide configuration parameters for GPIB address serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN The following tables represent the configuration switch settings for the modules without on board LAN only 26 1260 Binary Pump VL User Manual Introduction 1 Table 6 8 bit Configuration Switch without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 rsvo sys rsvo rsvo Fc The LAN settings are done on the LAN Interface Card G1369A B Refer to the documentation provided with the card Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position d
123. t blocked the damper 3 Defective pump drive assembly Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative 102 1260 Binary Pump VL User Manual Error Information 7 Motor Drive Power Motor Drive Power Pump channel A B Motor Drive Power Pump channel B The current drawn by the pump motor exceeded the maximum limit Blockages in the flow path are usually detected by the pressure sensor in the damper which result in the pump switching off when the upper pressure limit is exceeded If a blockage occurs before the damper the pressure increase cannot be detected by the pressure sensor and the module will continue to pump As pressure increases the pump drive draws more current When the current reaches the maximum limit the module is switched off and the error message is generated Probable cause Suggested actions 1 Flow path blockage in front of the damper Ensure the capillaries and frits between the pump head and damper inlet are free from blockage 2 Blocked outlet valve Exchange the outlet valve 3 High friction partial mechanical blockage in Remove the pump head assembly Ensure there the pump drive assembly is no mechanical blockage of the pump head assembly or pump drive assembly 4 Defective pump drive assembly Please contact your Agilent service representative 5 Defective main board Please contact your Agilent service representative
124. te timeout 89 selection valve failed 97 selection valve fuse 100 servo restart failed 106 shut down 88 216 stroke length 111 synchronization lost 89 temperature limit exceeded 102 temperature out of range 101 timeout 88 error message wait timeout 113 error solvent zero counter 94 Exchanging the Active Inlet Valve Cartridge 166 exchanging active inlet valve 164 164 active inlet valve 133 166 inlet valve 151 interface board 168 outlet valve sieve 153 outlet valve sieve 133 outlet valve 133 153 pistons 133 146 pump seals 143 pump seals 133 purge valve frit 133 purge valve 133 solvent selection valve 157 wash seals 160 wash seals 133 147 external contact cable 202 F fan failed 92 fastest gradient response 75 firmware updates 1 0 170 upgade downgrade 1 0 upgrade downgrade 1 0 flow accuracy 36 flow connections 54 57 flow precision 36 flowrange 36 frequency range 35 G gas solubility 64 general error messages 88 gradient formation 36 hexagonal key 3mm 140 146 149 hexagonal key 3mm 147 hexagonal key 4mm 140 146 149 hexagonal key 4mm 147 highest injection precision 72 high pressure mixing 8 hints for successful use 64 humidity 35 hydraulic path with solvent selection valve 186 hydraulic path without solvent selection valve 188 hydraulic system 36 index limit 108 index adjustment 109 index missing 110 initialization failed 112 initialization 12 12 inlet valve 15
125. tem Repair rear panel and connectors Description Part Number 1 FSi wl nr ol Nut M14 analog output Washer analog output Standoff remote connector Standoff GPIB connector Power supply behind rear panel Screw M4 7 mm lg power supply Washer power supply p n 2940 0256 p n 2190 0699 p n 1251 7788 p n 0380 0643 p n 0950 2528 p n 0515 0910 p n 2190 0409 1260 Binary Pump VL User Manual Parts for Maintenance 10 Pump Head Assembly Without Seal Wash Item p n 1 5067 4695 2 G1312 60062 3 G4220 63015 4 G4220 24013 5 G1312 67300 6 0905 1503 7 G1312 25260 8 0515 0175 9 G1312 23200 10 G1312 60061 11 G1312 60067 12 5042 1303 13 G1312 60066 14 G1312 23201 15 0515 2118 1260 Binary Pump VL User Manual Description Sapphire piston default Piston housing incl spring Support Ring without Seal Wash Backup Ring for Support Ring Capillary outlet valve to piston 2 Piston seal PTFE carbon filled black pack of 2 default Pump housing Mounting screw for manual purge valve holder M4 20 mm long Holder for manual purge valve Purge valve assembly Outlet valve complete Screw lock Passive inlet valve Adapter Screw M5 60 mm long 175 10 Parts for Maintenance Pump Head Assembly Without Seal Wash Figure 30 Pump Head Assembly Without Seal Wash 176 1260 Binary Pump VL User Manual Parts for Maintenance 10 Pump Head Assembly with Seal Wash Opt
126. ts are installed outlet valve or purge valve tightly Exchange the component if required 124 1260 Binary Pump VL User Manual Test Functions and Calibration 8 Pressure limit not reached but plateaus horizontal or positive Probable cause 1 Degasser and pump channels A and or B not flushed sufficiently air in the channels 2 Wrong solvent All plateaus negative Probable cause 1 Loose or leaky fittings 2 Loose purge valve 3 Leaky mixer if installed 4 Contaminated purge valve 5 Loose pump head screws in channel A or B 6 Leaking seal or scratched piston in channel A2 or B2 7 Leaking outlet valve in channel A or B 8 Leaky damper 1260 Binary Pump VL User Manual Suggested actions Purge the degasser and pump channels thoroughly with isopropanol under pressure use the restriction capillary Install isopropanol Purge the degasser and pump channels thoroughly Suggested actions Ensure all fittings are tight or exchange capillary Tighten the purge valve 14 mm wrench Tighten the mixer fittings and nuts Open and close purge valve to flush out contamination Exchange the valve if still leaky Ensure the pump head screws in channels A and B are tight Exchange the pump seals in both channels Check the pistons for scratches Exchange if scratched Exchange the outlet valve Exchange damper 125 126 Test Functions and Calibration First plateau negative or unstable
127. um Degasser 52 Connecting Control Software and or G4208 A Instant Pilot 53 Flow Connections of the Binary Pump with Solvent Selection Valve 54 Flow Connections of the Binary Pump Without Solvent Selection Valve 57 Priming and Purging the System 60 Priming with a Syringe 60 Priming with the Pump 62 This chapter gives information about the preferred stack setup for your system and the installation of your module ee Agilent Technologies 39 3 Installing the Pump Unpacking the Binary Pump Damaged Packaging CAUTION If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions Delivery Checklist 40 Delivery Checklist Ensure all parts and materials have been delivered with the binary pump The delivery checklist is shown in Table 15 on page 40 In order to identify parts you may refer to Parts and Materials on page 172 Please report missing or damaged parts to your
128. ump VL User Manual 2 Site Requirements and Specifications Site Requirements 32 Physical Specifications 35 Performance Specifications 36 This chapter provides information on environmental requirements physical and performance specifications ee Agilent Technologies 31 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 13 on page 35 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed CAUTION Unaccessable power plug In case of emergency it must be possible to disconnect the
129. users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is required between the pump and autosampler See Figure 10 on page 46 and Figure 11 on page 47 Instant Pilot Detector Column compartment Autosampler Fu ENX c S 7 E oo T 2 I Sais Thermostat for the ALS optional Solvent cabinet Degasser optional Pump Figure 10 Recommended Two Stack Configuration for 1260 Front View 46 1260 Binary Pump VL User Manual Installing the Pump 3 Optimizing the Stack Configuration LAN to control software CAN Bus cable to Instant Pilot Thermo cable optional Remote cable AC Power C
130. wth The presence of algae in the Agilent 1260 Infinity can cause the following to occur e PTFE frits PTFE frit pack of 5 p n 01018 22707 purge valve assembly and column filter blockage causing increased system pressure Algae appear as white or yellowish white deposits on filters Typically black particles from the normal wear of the piston seals do not cause the PTFE 1260 Binary Pump VL User Manual Using the Binary Pump 4 frits to block over short term usage Please refer to the section Exchanging the Purge Valve Frit or the Purge Valve on page 137 in this manual Short lifetime of solvent filters bottle head assembly A blocked solvent filter in the bottle especially when only partly blocked is more difficult to identify and may show up as gradient performance problems intermittent pressure fluctuations etc Algae growth may also be the possible source for failures of the ball valves and other components in the flow path How to Prevent and or Reduce the Algae Problem Always use freshly prepared solvents especially use demineralized water which was filtered through about 0 2 um filters Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle Solvent bottle amber p n 9301 1450 supplied with the instrument for your aqueous mobile phase If possible add a few mg l sodium azide or a few percent organic solvent to the aqueou
131. y binary pump to injection device to the outlet of the purge valve 1260 Binary Pump VL User Manual Bottle head assembly Solvent cabinet Tube clip Static Mixer Purge valve Pump head channel A Adapter Active inlet valve A Waste tubing Installing the Pump 3 Flow Connections of the Binary Pump Without Solvent Selection Valve 8 Purge your system before first use see Priming and Purging the System on page 60 py Outlet capillary to autosampler Pump head channel B Active inlet valve B Waste outlet Figure 17 Flow Connection of Binary Pump Without Solvent Selection Valve 1260 Binary Pump VL User Manual 59 3 Installing the Pump Priming and Purging the System If a degasser is installed it can be primed either by drawing solvent through the degasser with a syringe or by pumping with the pump Priming the vacuum degasser or system with a syringe is recommended when vacuum degasser or system is used for the first time or vacuum tubes are empty or e changing to solvents that are immiscible with the solvent currently in the tubes Priming the system by using the pump at high flow rate 8 5 ml min is recommended when pumping system was turned off for a length of time for example overnight and if volatile solvent mixtures are used or e solvents have been changed Priming with a Syringe WARNING When opening capillary or tube fittings solv
132. y pump 1 G1312 68711 Active Seal Wash Option kit Binary pump Preparations Switch off the pump at the main power switch Remove the front cover Remove the top cover and foam 1 By using a screwdriver remove the metal plug in the z panel Az Figure 21 Removing the metal plug from the z panel 2 Insert the socket delivered with the Seal Wash pump assembly into the hole on the z panel 160 1260 Binary Pump VL User Manual Maintenance 9 3 Guide the wire of the active seal wash assembly through the hole and screw it onto the z panel 4 Guide the wire over the fan and plug the connector onto the mainboard connector P7 D 2 jo gt gt X ESTA SK S Le E Cb Y ILS f YEN Figure 22 Wire connected to the mainboard 5 Replace the foam and top cover 6 Disconnect all capillaries and tubes from the pump head and disconnect the active inlet valve cable 7 Using a 4 mm hexagonal key stepwise loosen and remove the two pump head screws and remove the pump head from the pump drive 8 Place the pump head on the backside of the plungerhousing on a flat surface Loosen the lock screw two revolutions and while holding the lower half of the assembly carefully pull the pump head away from the plunger housing 9 Remove the support rings from the plunger housing and lift the housing away from the plungers 1260 Binary Pump VL User Manual 161 9 Maintenance 10 Install the support ring assembly

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