Home

RMP Operating Manual - Frank`s Hospital Workshop

Contents

1. Dialog screen Sample Allocation appears Select or several tests by clicking on them Define the position of the first sample to be tested Start Rack and Start Container Enter the number of samples to be tested of Samples Click Repeat the above untill the samples are allocated to the tests that will be processed in the run to start Click Next gt All Samples All samples will be allocated to all tests No Samples Clear current allocation Dialog screen Worklist appears To disable specific samples test will not be performed Select sample to be disabled by clicking on it Click Enable Disable Sample Click Next gt current number of samples to be tested Rejected Samples number of samples including disabled samples To sort click column header Samples Dialog screen appears To specify a partly used testkit Select the testkit to be specified by clicking on it in the number of destination containers that can still be processed with the kit Click Recalculate Click Next gt September 2000 e 6 Operation Setting up a Run No Worklist Plate Usage Optimization Dialog screen Plate Usage Optimization appears Click on one of the microplates to view the the plate layout Select a sample to
2. GENESIS RMP Operating Manual Docu No 390 840 V3 0 September 2000 September 2000 Preface Preface General This manual provides basic information about the use operation and maintenance of the instrument for the user Before operating the instrument the user must read and understand this manual TECAN Schweiz AG regularly offers Operator Training Courses We highly recommend to attend such a course prior to working with the TECAN Instrument Information provided by TECAN is believed to be accurate and reliable However the user is responsible for the proper and correct use of the instrument No license is granted by acquisition of the instrument for any patent or patent rights of TECAN If the user does not follow the instructions given in this manual TECAN does not take any responsibility for injuries or damages caused by the TECAN Instrument Purpose of this document This Manual is intended to instruct the operator how to operate the instrument and do repairs and maintenance on an operating level Operating Documentation GENESIS RMP Operating Manual P N 390 840 GENESIS RMP TOPS Software Manual P N 390 837 GENESIS Maintenance and Service Logbook 390 924 Installation and Servicing Installation servicing and reinstallation of the instrument shall only be performed by TECAN authorized service personnel Copyright The contents of this manual are property of TECAN Schweiz AG No part of t
3. Bos eee Be av ee es we Does 1 6 INCGUD ALOR 2 usum ek acc dr wed es acd x e Sed 1 6 e a RE ales aed bed ds dalton 1 6 8121279 2 ian oU ah tee en ce tara e ECRIRE 1 6 Washer esse 1 6 i3 93 pav 1 7 Standard 1 7 Accessories and Spare 1 7 Safety Instructions 2 1 2 1 Definition Seance on fe dci a 2 1 Target Croup SENE 2 1 Importance of the Safety 2 1 Warnings Cautions and 5 2 2 Barcode Scanner PosID 2 2 Use of Mobile 2 2 GENESIS RMP Operating Manual Doc 390 840 V3 0 TOC i TABLE OF CONTENTS TOC ii 3 1 3 2 3 2 1 3 2 2 3 2 3 3 2 4 3 2 5 3 3 3 3 1 3 3 2 3 3 3 3 3 4 3 3 5 3 3 6 3 3 7 4 1 4 2 4 3 4 4 45 4 6 4 6 1 4 6 2 4 6 3 4 6 4 Structure and Function 3 1 1 yeas ee 3 1 Liquid PT v 3 1 Principle of Functioning 3 1 oyster 3 2 Liquid Level Monitori
4. Dialog screen Testkits appears i aae t1 mss To specify a partly used testkit anc me DS Selectthe testkit to be specified by clicking on it Type in the number of destination containers that can still be processed with r Specify partly used Testkit the kit iir Click Recalculate unn Click Next gt Dialog screen Plate Usage Optimization 338888888888 12222222222522 appears Click on one of the microplates to view the the plate layout Select a sample to be disabled by clicking on it Disable the sample by clicking Enable Disable Sample Click Recalculate plate usage is recalculated after samples have been disabled E 960000000000 1900000000000 Click Next gt green controls yellow blancs red samples white not in use September 2000 GENESIS RMP Operating Manual Doc No 390 840 V3 0 6 11 S Opmton 7 Setting up a Run Worklist Dialog screen Schedule appears 1 3 EN 3 a 2 mear i i 7 E The schedule gives an overview of the pre calculated start and endtimes of the tests e Click Next gt Test Incubator Heat Up Step 1 Elisa Transport 2 Elisa Muli 2 01000 20030 Distribution 3 Common Prediution 00000 01000 Shake 2 Elisa Muti 1 07400 In
5. Perform the daily maintenance procedures If not all barcodes could be read see paragraph 6 2 3 5 GENESIS RMP Operating Manual Doc No 390 840 V3 0 6 5 6 Operation Setting up a Run No Worklist Sample Allocation All Samples No Samples Mouse Zoom Zoom Out 005900909000099 4 mns Test Container No of Samples 1 Elsa _ Grid E Tube 13 100mm 16 Pos 11 12 2 Eisa Mul Grid Tube 13 100mm 16 Pos 151 36 3 Elisa Muti 2 Grid 9 Tube 13 100mm 16 Pos 31 20 Elisa Muli 1 Tube 13 100mm 16 Pos EE Stat Container 151 7 Noof Samples 36 OE ces v 1000601 Elisa 1000602 Elisa v 1000603 Elisa 1000604 Elisa v 1000605 Elisa v 1000606 Elisa v 1000607 Elisa v 1000608 Elisa 1onnena Flea irn wil a Test Samples Rejected Samples Enable Disable Sample Elisa 12 0 Elisa Multi 1 36 0 Elisa Muli 2 20 0 Print Worklist Testkit Desciipl Balch No Samples Container Elisa New test ki T 12 17 Elisa Muli 1 New lest ki 1 36 40 Elisa Muti 2 New test 20 25 Specify partly used Testkit Testkit Test Elisa Elisa Remaining destination containers 0 Recalculate GENESIS RMP Operating Manual Doc 390 840 V3 0
6. Set the Z axis offset using the Instrument Software see Instrument Software Manual No 390 791 if the tips differ for more than 0 5 mm in the Z vector after the instrument is initialized Flush all tips with 2 M HSO see paragraph 7 3 6 4 Figure 7 6 Exchange Fixed Tips GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 7 3 6 4 7 3 6 5 7 3 6 6 7 3 6 7 September 2000 7 Maintenance Flushing new Tips with 25 To flush the tips with 2M H5SO run the Tip Decon Profile supplied with the original database see also paragraph 6 2 4 1 Place a 16 positions carrier with 8 13 100 tubes filled with 50MM NaCl solution on grid 10 These vials will be used as samples 2 Place another 16 positions carrier with 8 empty 13 100 tubes on grid 11 This is the destination carrier 3 Place the Wash station on grid 13 of the Worktable 4 Filla 100ml trough with 2M H2SO and place it on the rear position of a through Rack on grid 14 5 Run the Tip Decon Profile see paragraph 6 1 Disposable Tip DiTi Adapter Cleaning DiTi Cones Before switching on the GENESIS RMP use a lint free tissue soaked in ethanol 7096 or isopropanol to clean the DiTi adapters Tightening DiTi Cones Tighten the Diti cones with the help of the cone wrench see figure 7 7 Figure 7 7 Tighten
7. 4 Unreadable barcode label wrong barcode damaged label etc If the PosID failed to read sample barcodes do the following 1 Make sure that all barcode labels were placed correctly and are undamaged 2 Make sure that all tubes and racks were placed on the worktable correctly 3 Reread the barcode labels If the PosID still fails to read the sample barcodes manually enter the sample ID into the TOPS software or continue with Next In the second case TOPS will use the rack and sample position as sample barcode Setting up a Run Options To speed up the start up procedure of the TOPS run module some of the dialogs may not need to show up in each start procedure The dialogs may be skipped by unchecking the boxes of these dialogs see figure 6 6 Figure 6 6 Options Select Start Up Wizard Pages TOPS Run Control Demo Elisa Fie Bun Instrument Refill Results View Window B Peale Se El Select start up wizard pages x and formano Identify Worktable Cancel Sample Allocation Worklist Testkits Plate usage optimization Schedule Load Testkits and disposable Racks recommended Load Destination Plates recommended Load Global Liquids recommended Load Diti recommended GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 e 6 Operation Running a Profile Introductio
8. GENESIS RMP Operating Manual Doc No 390 840 V3 0 6 21 6 Operation N End of Run Introduction 6 2 5 End of Run 6 2 5 1 End of Run Introduction At the end of a run the TOPS Run Control gives a test status overview figure 6 9 TOPS produces the following test results Test error report see paragraph 6 2 5 2 Pipetting status report see paragraph 6 2 5 3 Test result files see paragraph 6 2 5 4 Figure 6 9 Test Status Overview Test Status Overview x Errors Wamings Pipetting Errors v 1 Elisa Done 0 0 2 Elisa Muki 1 Done 11 0 11 2 Elisa Multi 2 Done 1270 12 3 Common Predilution Done 0 0 0 Test Details Plate Details Print List 6 2 5 2 End of Run Test Error Report A test error report lists all actions of a test and gives an overview of errors that occurred during the processing of the test To view the test error report Select a test in the test status overview figure 6 9 Click Test Details 6 22 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 6 Operation End of Run Pipetting Status Report 6 2 5 3 End of Run Pipetting Status Report A pipetting status report lists all positions with the liquid name and the pipetting status To view the pipetting status report In the test status overview figure 6 9 select a test Click Plate Details The pipetting status consist of 6 code numbers
9. Liquid system leaky Gas evolution Liquid system leaky System liquid bottle empty Waste container full Waste tubing blocked Not enough liquid Bad ground connection of rack carrier Bad connection of ILID cable to board System liquid bottle empty Not enough DiTi s in DiTi rack Wrong tip position coordinates Tip check switch not working Wet or unclean cone DiTi adapter incorrectly mounted Tip check switch not working X Y or Z drive blocked X Y or Z drive blocked To Do Follow the instructions given in 7 3 1 1 Follow the instructions given in 7 3 1 1 Flush liquid system see 7 8 1 2 Follow the instructions given in 7 3 1 1 Refill system liquid Empty waste container Unblock waste tubing Add liquid Make sure that the worktable is dry Place rack correctly on carrier Call authorized Tecan service technician Refill system liquid Place DiTi s at specified position Call authorized Tecan service technician Call authorized Tecan service technician Clean DiTi cone with ethanol 70 Call authorized Tecan service technician Call authorized Tecan service technician Clean check for obstacles see 7 3 5 1 Clean check for obstacles see 7 3 5 1 September 2000 6 3 3 RoMa Symptom Plate not fetched Initialization error Collision error detected 6 3 4 PosID Symptom Barcode not read PosID initialization error 6 3 5
10. Possible Cause No plate on carrier Gripper lost plate RoMa too far on one side Gripper completely opened closed Collision Motor blocked Possible Cause Barcode not facing reader Bad barcode quality Barcode not positioned properly Barcode not identified Barcode not defined X Y or Z drive blocked Heated Shaking Incubator 6 Operation End of Run Test Result Files To Do Place plate on carrier Call authorized Tecan service technician Place RoMa in the middle of the instrument and re initialize Call authorized Tecan service technician Call authorized Tecan service technician Call authorized Tecan service technician To Do Place tubes racks and carriers with the barcode in the right direction see also 8 6 3 4 Replace barcode with new barcode Place barcode properly see S 6 3 4 Make sure that the barcode is positioned properly Call authorized Tecan service technician Call authorized Tecan service technician Clean check for obstacles If the shaker unit does not work or fails to initialize call an authorized Tecan service technician If the slot doors keep open or a temperature overload occurs call an authorized Tecan service technician September 2000 GENESIS RMP Operating Manual Doc No 390 840 V3 0 6 Operation End of Run Test Result Files 6 3 6 6 3 7 6 3 8 Washer Symptom Plate not washed regularly Fill verification during
11. correctly call authorized Tecan service technician Define filter Define filter Change filter carriage or check filter values for incorrect input September 2000 7 1 7 1 1 7 2 7 2 1 7 2 2 7 Maintenance Maintenance Introduction Safety Instructions Carefully read the safety instructions in chapter 2 before performing any maintenance procedures Personnel Qualifications Maintenance procedures as described in this manual are to be performed by the operator for operator qualifications see paragraph 6 1 2 unless mentioned otherwise Schedule General Directions The daily and routine maintenance schedule presented here are general guidelines The schedule may need to be adapted for special conditions of your laboratory We strongly recommend to prepare a maintenance checklist in your mother language Daily Maintenance Wipe up all spills immediately Do not allow the pumps to run dry September 2000 Item Action When How Liquid system Check for leaks Before switching on 7 3 1 1 Syringes Tighten syringe and Before switching on 7 3 2 1 plunger lock screw Fixed tips Clean Before switching on 7 3 6 1 Check for damaging Before switching on 7 3 6 2 Tighten tip nut Before switching on 7 3 6 3 DiTi Cones Clean Before switching on 7 3 6 6 Tighten Before switching on 7 3 6 7 Liquid system Flush Before each run S 7 3 1 2 Washer Rinse day At the beginning of each 7 3 3 1 day o
12. gt 50 ul microplates with round bottoms gt 20 ul conductive liquid microplates with round bottoms Each tip is connected to an integrated liquid detector module ILID Tips detect the surface of conductive solutions upon contact The liquid detectors operate by monitoring the capacity difference between the tip and the instrument working surface When a tip touches the liquid surface the ILID detects the change in capacity and triggers a detection signal A signal is produced both when moving into a liquid and when coming out This feature allows the detection of aspirated blood clots The software stores the position at which the liquid detection signal did occur Using the aspirated volume and the vial diameter it calculates the point at which the liquid detection signal should be interrupted A tolerance for cohesion and hysteresis effects is taken into account If the interuption signal occurs past this tolerance it is assumed that a clot has been aspirated The liquid detection feature offers the following advantages Minimum submerge depth of the pipette tips Minimal tip contamination and therefore only limited need for tip washing Appropriate message if no liquid or not enough liquid available for sampling Software controlled constant submerge depth during aspirating and dispensing Clot detection GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 3 2 5 3 3 3 3 1 September 2000
13. 1 3 7 1 3 7 1 September 2000 1 Product Description Environmental Conditions Storage Temperature 1 60 C 34 140 F Storage Humidity 5 80 relative humidity at 30 C 86 F Operation Temperature 18 30 C 65 86 F Operation Humidity 30 80 relative humidity at 30 C 86 F non condensing Operational Height Operation allowed up to 2500 m 8202 ft above sea level Pollution Degree 2 Use Indoor use only Emissions Noise Maximum 70 dBA 1 m Modules Liquid System Volumes Volumes in the range from 3 ul to 5000 ul depending on syringe size can be pipetted with standard tips and 3 to 1000 ul with disposable tips Liquid Detection Less than 50 ul of conductive liquid in round bottom microplates can be detected Precision Precision in single pipetting mode with de ionized water Volume Tip Precision 10 ul Standard DiTi 200 CV 396 100 ul Standard DiTi 200 DiTi CV 0 596 1000 Carryover Carryover is prevented best if disposable tips with filter are used With stainless steel Teflon coated tips and use of the Monitored Pump Option MPO carryover of less than 10 8 can be achieved GENESIS RMP Operating Manual Doc No 390 840 V3 0 1 5 1 Product Description 1 3 7 2 Diluter Resolution 3000 steps per syringe stroke Plunger Speed 1 2 sec stroke to 10 min stroke Syringe Size 250 ul 5 0 ml 250 ul 5 0 Plunger Travel Lenght 30 mm 1 3 7 3 Incubator Temperature Range Ro
14. 200 DiTi 1000 96 DiTi 200 96 DiTi 1000 96 niri 1000 Tips Complete Columns 4 Tips in incomplete Column 3 Set Set to Full Start Run Press Next to start the run Te a lt Back Newt 6 Operation Setting up a Run Worklist Dialog screen DiTi appears only if a profile was chosen that uses DiTi s Select the DiTi rack to be defined by clicking on it Typeinthe number of Complete Columns and the number of Tips in incomplete Column Click Set Ifthe selected rack is full click Set to Full All Full All racks will be set to full Disposable Tips are always picked from left to right and from rear to front Make sure that partly used DiTi racks are filled up in this direction Dialog screen Start Run appears e Click Next gt The run is started See paragraph 6 2 4 GENESIS RMP Operating Manual Doc No 390 840 V3 0 6 13 6 Operation TECAN Setting up a Run Worklist Query Mode with Worklist Editor 6 2 3 4 Setting up a Run Worklist Query Mode with Worklist Editor To set up a new run proceed as follows Start TOPS Run Control by double clicking the Run Control Icon 5 Run Control RunCtrl1 The Run Control main menu appears File Run Instrument Refill Results View Window Options Hel 1 Bene PH eee e Cli
15. 870 mm 80 77 1230 mm 48 4 RMP 100 1856 mm 73 1 RMP 150 2230 mm 87 8 RMP 200 2830 mm 111 45 city 840 mm September 2000 GENESIS RMP Operating Manual Doc No 390 840 V3 0 1 3 1 Product Description 1 3 2 Worktable Dimensions X range RMP 100 X range RMP 150 X range RMP 200 Y range Z range 1 3 3 Weight RMP 100 with PosID RMP 150 with PosID RMP 200 with PosID 1 3 4 Supply Connections Primary Voltage Secondary Voltage Line Frequency Power Ground Line Circuit Breaker Voltage Fluctuation Overvoltage Category 550 mm 21 6 24 grids 925 mm 36 4 39 grids 1525 mm 60 0 63 grids 418 mm 16 5 total range from first to last tip 364 mm 14 35 common range for all tips 4 tip arm 292 mm 165 mm 185 mm 150 mm 11 5 common for all tips 8 tip arm 6 5 with standard tip 7 3 with DiTi 200 ul 5 9 with 1000 ul aT lt lt 166 kg 365 Ib 188 kg 414 4 Ib 241 kg 531 3 Ib 100 240 VAC 24 VDC 50 60 Hz 1000 VA Must be connected 10A 1096 and 15 Class 2 To prevent interruptions in powersupply the RMP powersupply and computer must always run via an online UPS Output UPS 1500 W 2000 VA 100 240 VAC Recommended back up time 8 15 min Input voltage 200 240 VAC Phase Single Phase 1 4 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 1 3 5 1 3 6
16. Descrip Batch No Samples Container iig E entes gov To specify a partly used testkit Elisa Multi 2 New test kit 1 20 25 Selectthe testkit to be specified by clicking on it Type the number of destination containers that can still be processed with r Specify partly used Testkit the kit Testkit Test Elisa Elisa Click Recalculate Remaining destination containers 0 Recalculate Click Next September 2000 GENESIS RMP Operating Manual Doc No 390 840 V3 0 6 15 6 Operation Setting up a Run Worklist Query Mode with Worklist Editor 00000000 00000000 00000000 00000000 00000000 00000000 00000000 00000000 OOOOOOOO Elisa Enable Disable Sample Recalculate Back Schedule BN Incubator Heat Up Step 1 Elsa Transport 2 Elica Muti 2 01000 20030 Distribution 3 Common Prediution 0 00 00 01000 Shake 2 Elisa Muli 1 01000 20030 Incubation Heated Plate Wash z tw Loading of Testkits and Disposable Racks x EEREN Rack Type Poston into GENESIS RMP Operating Manual Doc No 390 840 V3 0 TECAN Dialog screen Plate Usage Optimization appears Click on one of the microplates to view the the plate layout Select a sample to be disabled by clicking
17. Diode It is generally used to visually signal the status of an electronic board Liquid Detector ILID Liquid Class A set of properties defining a theoretical model of one type of liquid Identified by a generic name e g Serum Buffer Ethanol etc it includes all default Liquid Handling Parameters required to process liquids of this type Liquid Handling Arm RSP component mounted to the X slide containing and holding the pipetting tips Liquid Handling Parameters The set of parameters which influence the liquid distribution process e g aspirate and dispense speeds delays tracking etc Liquid System All instrument modules and parts which contain or directly influence liquid tubing diluters valves tips etc Local Liquid A liquid used for one test only and which is placed into a test specific transportable reagent rack Microplate A plate of standardized size usually containing 96 containers well Module Software A unique application with its own icon in the program manager Multi pipetting The Pipetting Mode where one aspiration is performed for aliquoting into several destination positions Offset After the RSP detects the initialization signal in one of the three axes during the initialization cycle it moves back a certain distance This distance is the offset Partition Volume The volume of liquid between two airgaps which separates the System Liquid from the liquid to be aspirated It is wasted after us
18. Operating Manual Doc No 390 840 V3 0 September 2000 September 2000 Glossary System Liquid A liquid which fills the Liquid System and is used as wash fluid and or can be added to several all Samples analogously to the Additive Test A sequence of Actions that is performed automatically and gives a result that is automatically measured Testkit An assembly of liquids and consumables necessary to make an assay Tip Touch Dispensing Dispensing with tip touching the liquid in the destination container Tracking Speed Refers to the speed at which the tip moves up or down to follow the liquid level during aspiration and dispense operations Volume Calibration Function The software function by which the volumes actually pipetted can be calibrated in order to correspond exactly to the volume intended Wash Means to aspirate system liquid from the diluent reservoir and dispense it through the system into the wash position to clean the inside and the outside of the pipetting tip Wash Station Generally referred to as the physical combination of a Cleaner position and a Waste position Wash to Waste The procedure which removes remainders of liquid from within the tip and the Liquid System to prevent carryover It is executed after each or a few Pipetting Cycles within a Distribution and the wash liquid is discarded into the Waste position GENESIS RMP Operating Manual Doc No 390 840 V3 0 11 Glossary 12 Wash to Clea
19. and wait until the status changes to UNLOCKED Select the rack to be refilled by clicking on it Type in the number of Complete Columns and the number of Tips in incomplete Column Click ee EE Ifthe selected rack is click to Full Cancel Heb e Click Continue The run continues All Full All racks will be set to full Disposable Tips are always picked from left to right and from rear to front Make sure that partly used DiTi racks are filled up in this direction GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 6 2 4 4 6 2 4 5 September 2000 6 Operation Running a Profile Manually Pipetting of Common Pre Dilution Running a Profile Manually Pipetting of Common Pre Dilution If an error s occurs during the pipetting of a common pre dilution e g because not enough liquid was available in the sample tube an alarm goes off after the pipetting procedure has been finished Proceed as follows Click Instrument Access and wait until the s E r1 1 status changes to UNLOCKED M Manually pipet the missing samples into ij the p
20. axis defining rotational movements figure 3 3 RoMa Gripper figure 3 3and 3 4 moves clockwise from 0 270 figure 3 4 A dead sector of 90 between 270 and 360 cannot be accessed by the RoMa Gripper figure 3 4 Figure 3 3 RoMa Coordinate System 1 Rotator 2 Gripper 3 Gripper fingers Figure 3 4 RoMa Coordinate System Dead Sector 270 Dead Sector 1 Gripper 3 4 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 3 3 3 3 3 4 3 3 5 September 2000 3 Structure and Function Positive Identification PosID The optional positive identification reads barcodes on carriers racks and containers With its gripper it pulles carriers towards the rear of the instrument for barcode identification on tubes and microplates and than pushes the carrier back into the operating position Figure 3 5 Positive Identification PosID Scanner head output window with laser beam of laser class 2 Gripper No tube sensor Colombus Microplate Washer The microplate washer is loaded and unloaded by the RoMa and can be equipped with an 8 fold or 16 fold wash head with 1 aspirating and 1 dispensing needle for each well During the wash of a microplate the fill verification can be activated for each individual nozzle of the wash manifold to detect possible clogged tips Four different wash liquids holding 2 liters each can be placed on the front rack of the add on module A hall sensor
21. be disabled by clicking on it Disable the sample by clicking Enable Disable Sample Click Recalculate plate usage is recalculated after samples have been disabled 066000000000 0000000 Elisa Enable Disable Sample Recalculate Back Click Next gt green controls yellow blancs red samples white not in use Schedule x 1 mr 3onmN 3 E mm XN Dialog screen Schedule appears The schedule gives an overview of the pre calculated start and endtimes of the tests e Click Next gt Test Incubator Heat Up Step Elisa Transport Elisa Multi 2 01000 20030 Distribution Common Predilution 0 00 00 0 10 00 Shake Elisa Muti 1 01000 20030 Incubation Heated Plate Wash zi cue uen cea MON Loading of Testkits and Disposable Racks x Dialog screen Loading of Testkits and Disposable Racks appears Place all testkit racks destination racks predilution racks transportable reagent racks on the worktable as indicated on the screen Click Next September 2000 GENESIS RMP Operating Manual Doc No 390 840 V3 0 6 7 S Opmton 7 Setting up Run Worklist Dialog screen Destination Plate Loading Ambient Incubator appears Load the destination plates as
22. make backup folders at the end of a run The frequency in which this occurs can be set as follows ES TOPS Run Control Demo Elisa File Instrument View Window To set the backup configuration B S 2 t View Results l Choose the path where backup folders Backup Configuration to be stored Set the backup reminder Result Backup Configuration x Backup Folder Root OK D GEN32 TOPSSRESULTS Browse Cancel Backup Reminder at End of each Profile Weekly Frefered dey ofthe week Friday 71 Monthly r Optional Backup Utility Browse 9 Run Control BunCtl1 File Instrument Refill View Window Optio B amp l 1 Backup Backup Configuration To move all result files to a backup folder Click Move Result Files If Exit is clicked the files are not moved IDLE Errors Watt Move Result Files to Backup Folder Moves all result files to a subfolder of the configured backup folder The subfolder gets named as YYMMDDXX Where Yr MMDD is the backup date and XX is a daily count up index Move Result Files The Move Result Files to Backup Folder Status 2 dialog periodically appears after run as set in the backup configuration see above Configured Backup Folder D GEN32 TOPS RESULTS 6 2 7
23. n T GENESIS RMP Operating Manual Doc No 390 840 V3 0 Aspirating tubing Pressure clamps Liquid distributor Interconnect tubing Diluter Pipetting tubing 2 Pipetting tip Wash station Waste tubing Waste container 3 Structure and Function amp 3 2 2 3 2 3 3 2 4 System Liquid System liquid refers to a liquid that fills the liquid system and is used as wash fluid and or can be added to several or all samples analogously to the additive Standard system liquid De ionized water or aqueous buffer solution Saline Liquid Level Monitoring The system liquid level and the waste liquid level are monitored with the help of the Liquid Container Supervisor LICOS The LICOS is a tube closed at one end with a pressure sensor It is placed upright in the liquid sytem bottle and waste bottle When the liquid container is empty the pressure in the tube equals ambient pressure Increasing liquid levels in the liquid container increase the pressure on the aircolumn in the LICOS tube which is detected by the pressure sensor By calibrating the LICOS with an empty and full liquid container the pressure values for empty and full are set Liquid Detection Each pipetting channel has an individual liquid detection Generally detection of conductive liquids of following volumes is possible
24. on it Disable the sample by clicking Enable Disable Sample Click Recalculate plate usage is recalculated after samples have been disabled e Click Next gt green controls yellow blancs red samples white not in use Dialog screen Schedule appears The schedule gives an overview of the pre calculated start and endtimes of the tests e Click Next gt Dialog screen Loading of Testkits and Disposable Racks appears Place all testkit racks destination racks predilution racks transportable reagent racks on the worktable as indicated on the screen Click Next gt September 2000 September 2000 6 Operation Setting up a Run Worklist Query Mode with Worklist Editor Destination Plate Loading x Ambient Incubator Slot Container Test Info 5 HS Elisa Muli 1 1 ABD AS Elisa Muli 2 Plate Layout 21 He Global Liquids x Grid 22 RM 1 Grid 23 1 12 NC Elisa 1 Grid 23 3 36 NC Elisa 2 Grid 23 5 20 PC Elisa Grid 23 2 12 PC Elisa 1 Grid 23 4 36 PC Elisa 2 Grid 23 6 20 Predilution Diluent Grid 22 RR 1 DiTi 200 Grid 3 1000 96 Grid 9 DiTi 200 96 Grid 9 RM DiTi 1000 96 niri 1000 iei Set to Full Lime 0 GE
25. or error 3 Allows instrument access Starts a new run 5 Provides information about TOPS A 6 Allows to refill DiTi s Only active after instrument access 7 Color legend for run schedule Placement of global liquids 9 Placement of testkits and disposable racks 10 Overview of orders per test Further function of TOPS Run Control are described in chapter 6 and 7 of this manual 4 5 Malfunction Indicators Two LED indicators on top of the instrument CU board open the left access door indicate the state of the system Table 4 3 lists the different states and interpretation Table 4 3 LED Indicators Green LED Red LED Blinks ca 2 Hz Off No blinking No blinking Blinks ca 2 Hz Blinks a synchronously Blinks ca 2 Hz On Blinks ca 10 Hz Blinks ca 10 Hz Blinks ca 2 Hz Blinks ca 2 Hz 1 Hz 1 blink per second Meaning Status OK Fatal hardware problem Too many options connected EEPROM problem Firmware download in progress Powerfail of powersupply active 4 2 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 4 6 4 6 1 4 6 2 4 6 3 4 6 4 September 2000 4 Operating Keys Display Operating Modes Operation Modes Introduction TOPS Run Control can work in three operation modes see also paragraph 6 2 3 worklist Paragraph 4 6 2 e Worklist Paragraph 4 6 3 Worklist Query mode Paragraph 4 6 4 The operation mode canno
26. see figure 7 4 Figure 7 4 Washer Nozzles Unclogging cleaning rods r dispensing nozzle aspirating nozzle 7 3 4 Liquid Containers 7 3 4 1 Cleaning the Liquid Containers To prevent deposition of cristals and growth of micro organisms in liquid containers clean all liquid containers at least once a week Make sure to allow solvents e g ethanol to evaporate before filling reagents into the containers again Clean the waste containers at least once a day 7 6 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 7 3 5 7 3 5 1 7 3 6 7 3 6 1 7 3 6 2 September 2000 7 Maintenance RoMa LiHa Cleaning the Arm Guide In order to avoid uneven movements of the RoMa or LiHa clean the arm guide every week or earlier if required 1 Switch off the instrument 2 With a lint free tissue and a screw driver thoroughly clean the arm guide and the roller of both arms see figure 7 5 3 Manually move the arms and make sure the movement of both is even Caution The arm rails and arm guide rollers must be greaseproof for proper function Never use grease on the arm rails Figure 7 5 Arm Guide and Roller RoMa or LiHa Arm guide roller Arm rails Arm guide Fixed Tips Cleaning the Fixed Tips Before switching on the GENESIS RMP use a lint free tissue soaked in ethanol 70 or isopropanol to clean the fixed tips Make sure not to damage the tip
27. system is leaky do the following 1 Tighten tip nut see paragraph 7 3 6 3 or DiTi cones see paragraph 7 3 6 7 2 Tighten syringe and plunger lock screw see paragraph 7 3 2 1 3 Tighten tubing connections figure 7 1 4 Flush the liquid system see paragraph 7 3 1 2 and observe the tips or DiTi cones for 1 minute If no droplets are formed the liquid system is tight 5 If the system is still leaky call an authorized Tecan service technician Warning A leaky liquid system causes pipetting inaccuracy and cross contaminations Never operate the RMP if the liquid system is leaky 7 2 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 7 3 1 2 September 2000 7 Maintenance Figure 7 1 Tubing Connections Top view of instrument Tighten connections Tighten connections Caution The RoMa and LiHa are close together when the main cover is closed after tightening the tubing connections This causes collision when initializing the instrument Always separate RoMa and LiHa before initializing the GENESIS RMP Flushing the Liquid System When the liquid system is standing overnight gas evolution results in airbubbles in the liquid system Also during a run airbubbles remain in the liquid system Therefore we recommend to flush the liquid system before each run 1 Make sur that the System Liquid Bottle is full 2 Switch on the GENESIS RMP and start T
28. teh oad ears eas 7 7 Cleaning the Fixed 7 7 Checking Fixed Tips for Damaging 7 7 Replacing Fixed 7 8 Flushing new Tips with 2504 7 9 Disposable DiTi 7 9 Cleaning Cones 7 9 Tightening DiTi 7 9 Repair 8 1 Instrument Shut down Storage 9 1 Instrument shut 9 1 9 1 SIOIBS ion eddie d doit beet bah ack 9 2 Packing and Transportation 10 1 aa tok 10 1 PG IME PIX 10 1 Transportation 10 1 Disposal 11 1 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 e TABLE OF CONTENTS 12 Accessoires and Spare Parts 12 1 13 Customer Support 13 1 13 1 Customer Support on the Internet 13 1 13 2 How to reach us by 13 1 Decontamination Form A 1 B Pipetting Status 1 September 2000 GENESIS RMP Operating Manual Doc 390 840 V3 0 TOC v TABLE OF CONTENTS TOC vi GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 200
29. to make sure that there is enough wash liquid during a run supervises the liquid level in the bottles It is possible to place the wash liquids into more than one bottle If the first bottle gets empty the washer will switch automatically to the next one The microplate washer additionally offers the possibility of shaking plates in three different modes during the soak time of a wash cycle to enhance the wash procedure The CV of the dispensing pump is less than 496 Sunrise Spectra Microplate Reader The microplate reader is loaded and unloaded by the RoMa It can be used with four different filters simultaneously The filter wavelengths can be chosen between 340 nm and 700 nm The measurement range of the Sunrise Spectra is between 0 and 4 OD with linearity CV below 296 and a precision CV below 196 The accuracy of the reader is more than 99 Measurements can be done with a reference wavelength simultaneously When tests with colored reagent and sample addition monitoring systems are used a dispense check of the pipetting device can be defined as an individual action In addition four different shake modes ranging from 2 to 16 Hz with freely definable shaking times can be chosen to mix the liquid in the plates right before the measurement This shaking option of the Spectra can also be used for a short mix at any time during the process of a test GENESIS RMP Operating Manual Doc No 390 840 V3 0 3 5 3 Structure and Function amp
30. up a Run Introduction The GENESIS RMP instrument is operated via the TOPS Run Control software A run is set up based on a predefined profile that contains a selection of tests Three operation modes are available No worklist no external worklist available see paragraph 6 2 3 2 Worklist external worklist is imported see paragraph 6 2 3 3 Worklist query mode with worklist editor worklist generated manually see paragraph 6 2 3 4 The setup sequence for running a profile in TOPS Run Control is shown in figure 6 5 Figure 6 5 Setup Sequence for running a Profile in TOPS Run Control Select Profile Destination plates loading Identity Worktable Global Liquids No worklist Worklist query mode External H ni Worklist ES Load DiTi Sample Worklist Allocation m Editor a 23 Start Run gt Worklist a Run control screen Testkits Plate usage optimization Schedule l1 Loading of testkits and disposable racks GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 TECAN 6 2 3 2 September 2000 6 Operation Setting up a Run No Worklist Setting up a Run No Worklist To set up a new run proceed as follows Start TOPS Run Control by double clicking the Run Control Icon 0 5 Run Control RunCtrl1 The Run Control main menu appears File Run Instrument Refill Results View Window Op
31. 0 September 2000 Glossary Glossary Note This glossary explains terms used in any of the GENESIS Series Manuals or Software packages Terms explained here do not necessarily occur in the present Manual Accuracy The degree of conformity of a measure to a standard or true value difference of expected value and the actual value divided by the expected value multiplied by 100 Action Building blocks that can be used to define a Test Each action is performed by one hardware or one software module Additive A liquid e g reagent diluent taken from a Container on the work table and added to several or all Samples Standards Controls Blanks in order to cause or influence a reaction Additive Distribution A Distribution which adds an Additive to Destination containers which have already received liquid during a previous Distribution or are going to receive liquid in a Follow Up Distribution Application Generally refers to a software package with a specific purpose for example RIA EIA etc Aspirating Tubing The tubing leading from the reservoir of System Liquid diluent to the diluter valve Blank A position in a Destination Rack which does not receive Sample but only the Additive s It is used to determine the background signal in the detection or measurement system e g a photometer CAN Bus A communication standard for data transmission between computer and computer equipment For example internal communicatio
32. 0 September 2000 September 2000 6 1 6 1 1 6 1 2 6 2 6 2 1 6 2 2 6 2 3 6 2 4 6 2 5 6 2 6 6 2 7 6 3 6 3 1 6 3 2 6 3 3 6 3 4 6 3 5 6 3 6 6 3 7 6 3 8 TABLE OF CONTENTS Operation 6 1 INMOOUCHON She 6 1 Safety Instructions 6 1 Operator 6 1 Operation Perea 4 6 2 Instrument Setup 6 2 Placing Barcode Labels on Carriers Racks and Containers 6 2 Placing Carriers Racks and Containers onto the Worktable 6 3 Preparing the Pipetting 6 3 Switching on the GENESIS RMP 6 3 Setting up a 6 4 oes ected eh Slaw Las 6 4 Setting up a Run No 6 5 Setting up a Run 5 6 10 Setting up a Run Worklist Query Mode with Worklist Editor 6 14 Setting up a Run Unidentified 6 18 Setting up a Run 6 18 Running a Profile 6 19 Running a Profile 1 6 19 Running a Profile Handlin
33. 1 Maintenance 7 1 Introduction 7 1 Safety 5 7 1 Personnel Qualifications 7 1 Schedule 245 028 ha nie md 7 1 General 7 1 Daily 7 1 Routine 7 2 Half Yearly and Yearly Maintenance 7 2 Procedures ce 53921 er bete Rd io Red aed du 7 2 Liquid Systemi a aee dunt eed e dates ouside RR 7 2 Checking the Liquid System for 5 7 2 Flushing the Liquid System 7 3 Cleaning the Liquid 5 7 4 7 5 Tightening the Syringe and Plunger Lock 7 5 EIC PC LUTTE 7 5 Rinsing the 7 5 Unclogging 22165 7 6 Liquid lt 7 6 Cleaning the Liquid Containers 7 6 ss ska dated aaa RA RR 7 7 Cleaning the Arm 7 7 gd cip vat
34. 19 750 502 612 604 612 605 612 609 612 674 612021 520 629 Description Wash Station Carrier 8 Deep and 8 Shallow Positions Waste Tubing GENESIS PVC 12 x 9 mm length 2 5 m Carrier for Disposable Tips 2 DiTi Racks 96 Tips 1 Waste Position Carrier for Disposable Tips 3 DiTi Racks 96 Tips Waste Slide for Disposable Tips for use with rack 613 012 Carrier for Tubes 10 mm 96 Pos not for use with PosID Carrier for Tubes 18 mm 96 Pos not for use with PosID Carrier for Tubes 16 mm 96 Pos not for use with PosID Carrier for Tubes 10 mm 16 Pos Carrier for Tubes 18 mm 16 Pos set of 6 carriers set of 6 carriers Carrier for Tubes 16 mm 16 Pos set of 6 carriers Carrier for 3 Additive Troughs Tecan 100 ml 1 c u wide Custom Carrier Kit 1 Carrier Bottom 1 Glider 8 Screws Cooled Reagent Carrier Cooled Block for Tubes Cold pack set 4 pieces for 613 016 Carrier for Reagents Plastic Block Undrilled 3 c u wide for use on worktable only Rack for Reagents Plastic Block Undrilled 127 5 x 85 5 mm transportable with RoMa Abbott specific carrier for controls and reagents Carrier for Microplates RoMa 3 Pos Landscape Orientation Carrier for Microplates RoMa 2 Pos Portrait Orientation Set of 3 microplate holder XL size 128 5 mm x 86 5 mm Option for Carriers 612 604 and 612 605 Hotel for Microplates stores 9 MTPs Reagent Troughs 100 ml for use in 613 020 100 pieces Reage
35. 3 Structure and Function Wash Waste Station The wash waste station is placed on the worktable of the GENESIS RMP and is used for Cleaning of fixed tips between pipetting steps in order to reduce the possibility of carry over Discarding of waste liquid The wash waste station principle is shown in figure 3 2 Figure 3 2 Wash Waste Station Principle Waste position MA Wash position Waste drain Hardware System Modules The GENESIS RMP hardware is based on a modular design concept in which each of the major functions is performed by a modular component of the instrument Liquid Handling Arm LiHa The GENESIS RMP is standardly equipped with a liquid handling arm Four or eight pipetting tips are arranged on the LiHa that can move independently in Z direction up and down In Y direction front rear an equidistant tip spreading of 9 38 mm 0 31 1 5 inches is possible The complete liquid handling arm moves left and right X direction across the entire worktable GENESIS RMP Operating Manual Doc No 390 840 V3 0 3 3 3 Structure and Function 3 3 2 Robotic Manipulator Arm RoMa The robotic manipulator arm is used to transport microplates reagent blocks deep well plates etc to different positions on the worktable or for storage onto the shelf The RoMa coordinate system consists of four axes the X axis the Y axis and the Z axis defining linear movements and the R
36. 3 3 6 3 3 7 Room Temperature Incubator The darkened room temperature incubator for up to 6 microplates is accessible from the front for manual loading So the loading port and the room temperature incubator are the same device The RoMa has access to the microplates from the backside of the incubator to unload the plates when they will be used during a run The RoMa opens the doors passively when entering the incubator After leaving the incubator the doors will be closed automatically by a solenoid An automated locking mechanism locks the front door while the RoMa access the plates Plates will be placed back into the incubator for room temperature incubation or at the end of arun The six slots are separated completely from each other to prevent a potential influence of processed and unprocessed plates sharing a common atmosphere On the top of the room temperature incubator there is a loading port for taking out or placing back a microplate during a run if a manual step is defined in the test If you need to load more than 6 plates you can place another room temperature incubator on the left side of the first one This doubles your throughput capability to 12 plates Shaker Heated Incubator The shaker heated incubator is placed opposite the room temperature incubator at the backside of the instrument Six individually heatable slots allow different temperatures for each slot from 5 C above ambient temperature up to 50 C respectively
37. IS RMP Warning The PosID produces laser class 2 laser light This is harmfull to your eyes Do not look into the beam Prohibition The use of mobile phones in the vicinity of the ZA s RMP may cause false liquid detection Keep mobile phones out of the room in which a GENES RMP is installed no stand by operation allowed Operator Qualification Instrument operation requires thorough knowledge of applications instrument functions and software programs as well as all applicable safety rules and regulations The instrument shall be operated under consideration of this operating manual and by qualified and authorized laboratory personnel only TECAN regularly offers Operator Training Courses We highly recommend to attend such a course prior to working with the TECAN instrument GENESIS RMP Operating Manual Doc No 390 840 V3 0 6 1 6 Operation amp Placing Barcode Labels on Carriers Racks and Containers 6 2 Operation 6 2 1 Instrument Setup 6 2 1 1 Placing Barcode Labels on Carriers Racks and Containers Figures 6 1 6 2 and 6 3 show how to place the barcode stickers onto the carriers racks and containers such that the barcodes can be read properly by the Figure 6 1 Barcode Position on Tubes 1 gt gt 5mm 1 i v max 90mm max 117mn min 27mm QZ Quiet Zone Quiet zone white area on the barcode label Ne
38. Maintenance Mode as shown in figure 7 2 and put the tubing into a bottle with cleaning liquid see above Flush the liquid system twice as described in paragraph 7 3 1 2 Wait for at least 10 minutes to soak the system Place the tubing in a bottle with distilled or de ionized water and flush the liquid system two times as described in paragraph 7 3 1 2 5 Switch the valve back to Operating Mode see figure 7 2 6 Flush the liquid system eight times as described in paragraph 7 3 1 2 Figure 7 2 Clean Liquid System Operating Mode Maintenance Mode to fast wash pump from system liquid to fast wash pump container from bottle with cleaning liquid 7 4 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 e 2 7 Maintenance 7 3 2 Syringes 7 3 2 1 Tightening the Syringe and Plunger Lock Screws By the continuous up and down movements of the syringes during operation the syringe and plunger lock screws may loosen This causes leakage of the liquid system Therefore manually tighten the plunger lock screw and syringe screw figure 7 3 before switching on the GENESIS RMP Figure 7 3 Syringe and Valve Syringe Screw Plunger Lock Screw 7 3 3 Washer 7 3 3 1 Rinsing the Washer Rinse Day At the beginning of each day or when changing washing liquids rinse the washer with the washing liquid you are going to use or with distilled or de ionized water 1 Switch on the GE
39. NESIS RMP Operating Manual Doc No 390 840 V3 0 Dialog screen Destination Plate Loading appears Loadthe destination plates as indicated on the screen Toview plate layout select the plate to be viewed by clicking on it and click Plate Layout e Click Next gt Dialog screen Global Liquids appears Place the global liquids in the containers as indicated on the screen Click Next gt Dialog screen DiTi appears only if a profile was chosen that uses DiTi s Select the DiTi rack to be defined by clicking on it Typeinthe number of Complete Columns and the number of Tips in incomplete Column Click Set Ifthe selected rack is full click Set to Full All Full All DiTi racks will be set to full Disposable Tips are always picked from left to right and from rear to front Make sure that partly used DiTi racks are filled up in this direction 6 Operation TECAN Setting up a Run Unidentified Barcodes 6 2 3 5 6 2 3 6 Start Run x Dialog screen Start Run appears Click Next gt Press Next to start the run The run is started See paragraph 6 2 4 Setting up a Run Unidentified Barcodes The PosID may fail to identify sample barcodes due to 1 No barcode label on tube rack or missing tube rack 2 Barcode label placed incorrectly see figures 6 1 6 2 and 6 3 3 Tube placed incorrectly see figure 6 4
40. NESIS RMP and start TOPS Run Control 2 Inthe Run Control main menu select Instrument followed by Washer 3 Select the tab Rinse Day and apply the following settings Channel channel leading to the wash bottle containing the rinsing liquid M Duration 15 sec 4 Click Execute The washer is rinsed Rinse all channels At the end of each day rinse all channels with distilled or de ionized water Once per month rinse with distilled or de ionized water followed by ethanol followed by distilled or de ionized water 1 Switch on the GENESIS RMP and start TOPS Run Control 2 Inthe Run Control main menu select Instrument followed by Washer 3 Select the tab Rinse All Channels and apply the following settings e M Duration 15 sec 4 Connect all channels to the rinse liquid bottle containing water or ethanol 5 Click Execute The washer is rinsed 6 Select the tab Rinse Night and apply the following settings e Channel channel leading to the wash bottle containing water Duration 15 sec 7 Click Execute The washer is rinsed 8 Switch off the GENESIS RMP September 2000 GENESIS RMP Operating Manual Doc No 390 840 V3 0 7 5 7 Maintenance 7 3 3 2 Unclogging Nozzles To unclog the washer nozzles proceed as follows 1 Switch off the GENESIS RMP 2 Clean all nozzles by carefully pushing the cleaning rods into the nozzles The longer rod is for the aspirating nozzle and the shorter rod is for the dispensing nozzle
41. OPS Run Control 3 Inthe Run Control main menu select Run followed by Create New Run Dialog screen Select Profile appears 4 Select a profile and click Next gt Dialog screen Identify Worktable appears 5 Prepare the worktable as indicated and click The hardware is initialized and dialog Sample Allocation appears 6 Inthe Run Control main menu select Instrument followed by Liquid System Dialog screen Liquid System appears 7 Select the tab Flush Liquid System and apply the following settings Volume 30000 ul Speed 495 ul e Use MPO 8 Click Execute The liquid system is flushed 9 During flushing carefully observe the tubings If necessary gently move the tubings to make sure all airbubbles are removed 10 If there are still airbubbles in the tubings repeat steps 4 10 Warning Airbubbles in the liquid system cause pipetting inaccuracy Never operate the RMP with airbubbles in the liquid system GENESIS RMP Operating Manual Doc No 390 840 V3 0 7 3 7 Maintenance 7 3 1 3 Cleaning the Liquid System To prevent growth of micro organisms in the liquid system we recommend to clean the liquid system once a week Depending on your application you may soak the system with one of the following agents Weak detergent 2 solution Weak acid and base in sequence e 10 desinfective To soak the liquid system proceed as follows 1 Switch the valve to
42. Switching off The GENESIS RMP is switched off via the main power switch see figure 1 1 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 6 3 6 3 1 September 2000 Malfunction Troubleshooting Basic Instrument Symptom Possible Cause TOPS does connect to Instrument switched off instrument Instrument not connected TOPS runs in simulation mode Other LED indicators 6 Operation End of Run Test Result Files To Do Switch on the instrument Check cable connections Open RspDrv Log1 Under Options Driver untick the box Simulation Check LED indicators and call authorized Tecan service technician Two LED indicators on top of the instrument CU board open the left service access door indicate the state of the system If the green LED is blinking the status is OK Otherwise a fatal hardware problem occurred GENESIS RMP Operating Manual Doc 390 840 V3 0 6 25 6 Operation e End of Run Test Result Files 6 3 2 Liquid System Symptom Droplets hanging on fixed tips or DiTi cones Droplets coming out of syringes Airbubbles in liquid system tubing Wash station overflow No not enough liquid detected No system liquid detected No DiTi available DiTi not fetched DiTi not removed Initialization error LiHa Uneven movements LiHa GENESIS RMP Operating Manual Doc No 390 840 V3 0 Possible Cause Liquid system leaky
43. and Spare Parts Manuals Part No 390 840 1 109 004 1137701 1 139 003 390 837 390 924 390 791 Description GENESIS RMP Operating Manual Operating Manual for Colombus Washer Operating Manual for Sunrise Reader Remote Control Operating Manual for Spectra and Rainbow Readers GENESIS RMP TOPS Software Manual GENESIS Service and Maintenance Logbook GENESIS Instrument Software Manual Basic Instrument RoMa Part No 612 003 612 503 619 517 619 001 619 003 Description Transport Handles 1 Set Reference Tip stainless steel Cone Wrench for DiTi option Set of guide pins 15 guide pins 5 stop pins 2 reference pins Rubber sealing strip for worktable 200 cm Gripper finger left Gripper finger right GENESIS RMP Operating Manual Doc 390 840 V3 0 12 1 12 Accessoires and Spare Parts amp Liquid System Diluters Tips Part No Description 619 423 Syringe XP3000 0 25 ml 619 424 Syringe XP3000 0 5 ml 619 425 Syringe XP3000 1 0 ml 619 426 Syringe XP3000 2 5 ml 619 427 Syringe XP3000 5 0 ml 612 501 Standard Tip steel tip teflon coated outside without lock nut 612 504 Ceramic Tip stainless steel tip hard ceramic coating inside and outside without lock nut 619 515 Lock nut for standard and ceramic tip 619 502 Disposable Tip Option for 200 ul and 1000 ul tips 619 508 Set of sealing rings for DiTi options 612 510 Disposable Tips 200 ul conductive 2304 pieces 612 511 Disposable Tips 200 ul co
44. assigned to it or its barcode Racks can be of various types rectangular circular etc See also Class Rack class A set of properties e g number of rows columns offset etc of an arrangement of Container Classes as e g an MTP rack independent of its position on the worktable See also Class Ramping The acceleration and deceleration of motors to achieve smooth movement Rectangular Rack A physical arrangement of Containers whose dimensions are uniform Each row and column has the same number of containers and the distances between rows or columns are uniform Retract The process of pulling a tip back up after aspiration or dispensing GENESIS RMP Operating Manual Doc No 390 840 V3 0 9 Glossary 10 RoMa Robotic Manipulator Arm Component which grasps and moves objects within the working area of the instrument RS232 A communications standard for data transmissions between computer and computer equipment For example the instrument and the computer are connected by RS232 RS485 A communications standard for data transmission between computer equipment Run A sequence of processes on the instrument started by the user by selecting a method from a menu and ending with the return to this menu Sample Specimen of the substance e g blood serum urine etc to be analyzed by means of a Test Scheduler A software part which optimizes and connects tests of a run in order to minimize the overall run time S
45. ck the Create new run button Bi el rr Elsie Create new run and Information Instrument Access US Topic ToDo Abort CURIE I Dialog screen Select Profile appears Selected Profile SSS Select the profile to be run To set tests on priority select the test and click Priority LM cle ert Click Next gt QQ QC Elisa Si ac Elisa EAA No Com Pre Dilution QC Elisa QC Incubator iw Priority For tests set on priority the sample distribution is processed first Tip Decon BQ No Com Pre Diuion Tic Renown Identify Worktable x Dialog screen Identify Worktable appears Place all racks and carriers on the worktable as indicated on the screen Close the front access door Click Next gt Carter Type Grid Tube 13 100mm 16 Sample 648 Tube 13 100mm 16 Pos Sample Grid 9 Tube 13 100mm 16 Pos Sample 6810 Tube 13 100mm 16 Pos Sample Grid 11 Tube 13 100mm 16 Pos Sample Grid 12 Tube 13 100mm 16 Pos Sample Grid 13 Wash station Washstation zl If for the current run less sample carriers are needed than indicated on the screen place only the number of carriers required for this cack meo cm run onto the worktable The GENESIS RMP is initialized The PosID reads all ba
46. coating Checking Fixed Tips for Damaging Visually inspect the tip coating before switching on the instrument Use a mirror to have a proper view on the tip outlet Make sure that the tips are not bent If the tip coating is damaged or the tip is bent the tip must be replaced see paragraph 7 3 6 3 Warning Bent tips or damaged tip coating cause pipetting inaccuracy and liquid detection errors Never work with damaged or bent tips GENESIS RMP Operating Manual Doc No 390 840 V3 0 7 Maintenance 7 3 6 3 Replacing Fixed Tips Fixed tips must be replaced if they are bent or the coating is damaged To remove fixed tips proceed as follows 1 2 Switch off the GENESIS RMP and open the front access door Unscrew the tip nut see figure 7 6 Pull the pipetting tubing approximately 10 cm out of the tip adapter and pull tip off the tubing To install new fixed tips proceed as follows see figure 7 6 1 9 Pull down the pipetting tubing until it extends out of the tip adapter by approximately 5 10 Cut approximately 0 5 cm off the tubing an angle Remove the tube protecting the coated part of the tip Do not discard the protecting tube Make sure the tip coating is not damaged Carefully push the upper part of the tip 6 8 mm into the pipetting tubing Use sandpaper to hold the tubing Push the tip screw over the coated part of the tip Make sure not to damage the coating Manually tighten the tip nut
47. compuserve com Reading RG8 9AR Italy TECAN Italia S r l Telephone 39 2 267 00553 Via A Volta 16 Telefax 39 2 253 2890 20093 Cologno Monzese MI E Mail tecan tecan it Japan TECAN Japan Co Ltd Telephone 81 4 2334 8855 Meiji Seimei Fuchu Building 10F Telefax 81 4 2334 0401 1 40 Miya machi E Mail info tecan co jp Fuchu City Tokyo 183 USA TECAN U S INC Telephone 1 919 361 5200 P O Box 13953 Telefax 41 919 361 5201 Research Triangle Park NC 27709 E Mail tecan us tecan us com Asia TECAN Asia Telephone 65 444 18 86 26 East Coast Avenue Telefax 65 444 18 36 459 231 E Mail tecan tecan com sg Singapore September 2000 GENESIS RMP Operating Manual Doc 390 840 V3 0 13 1 13 Customer Support GENESIS RMP Operating Manual Doc No 390 840 V3 0 TECAN September 2000 September 2000 A Decontamination Form Decontamination Form TECAN Schweiz AG Feldbachstrasse 80 CH 8634 Hombrechtikon Switzerland Phone 41 55 254 81 11 FAX 41 55 244 45 83 http www tecan group com E mail tecan tecan ch Decontamination Form This form must be completed before a service engineer performs any service on the instrument or parts of it e g washer reader incubator etc If it is returned to the dis tributor the form must be added to the other shipping documents of the instrument components hereby declare that any parts in this package have never been expose
48. ction table 4 Cause of the error table 3 Pipetting action involved table 2 Liquid involved table 1 If no pipetting error occurred the pipetting status is 0 0 0 0 0 Table 1 Liquid Involved Error 0 Liquid affected No liquid Sample Control Additive Source in Predilution Mode Control in Predilution Mode Additive Predilution Multiple Cause Table 2 Pipetting Action Involved Error 00 01 02 03 04 05 10 11 12 September 2000 Error Name Unknown Action Aspirate Move Down Aspirate only Aspirate Retract Aspirate Clot Detec tion Aspirate Move in Z Dispense Move Down Dispense only Dispense Retract GENESIS RMP Operating Manual Doc No 390 840 V3 0 Explanation When the error occured there was no liquid being treated i e liquid was not directly affected Without Predilution Sample error With Predilution Predilution additive error Without Predilution Control error With Predilution Predilution additive error Additive was possible treated incorrectly The source liquid of a pre dilution was possibly affected Control or standard of a pre dilution was possibly affected The additive of a pre dilution was possibly affected Error condition not assigned to a particular liquid Meaning the error occurred somewhere on software level before aspiration while moving down no liquid during aspiration syringe drive blocked 2 after aspirati
49. cubation Heated Piste wash zi Loading of Testkits and Disposable Racks Dialog screen Loading of Testkits and Disposable Racks appears Place all testkit racks destination racks predilution racks transportable reagent racks on the worktable as indicated on the screen Click Next gt FESS Microplate deep well Destination Plate Loading x Dialog screen Destination Plate Loading appears Loadthe destination plates as indicated on the screen To view plate layout select the plate to be viewed by clicking on it and click Plate Layout Click Next gt Ambient Incubator gt 5 Elisa Multi 1 ABO AD Elisa Multi 2 Elisa Global Liquids Dialog screen Global Liquids appears Place the global liquids in the containers as indicated on the screen Mowezoom Click Next Zoom In quid Type Samples Batch No EvpiyDate 4 Conjugate Grid 22 AM 1 68 NC Elsa 5023 1 12 1 23 3 36 NCElsa2 Grid 23 5 20 FC Elisa Grid 23 2 12 PC Elisa 1 Grid 23 4 36 PC Elisa 2 Grid 23 6 20 Predilution Diluent Grid 22 RR 1 56 6 12 GENESIS RMP Operating Manual Doc 390 840 V3 0 September 2000 September 2000 DiTi
50. d to any hazard ous biological and or radioactive material or that such parts have been decontami nated or disinfected to remove or inactivate any biological and or radioactive material which could be dangerous to service personnel The parts have been decontaminated according to the decontamination instructions given in the appropriate instrument manual Instrument Type Serial No eee Parts which have been decontaminated tick appropriate box Tip Tip tubing Worktable Reader Syringes Incubators Washer Pipetting waste Washer waste Wash station PosID Interior surfaces Use only 70 ethanol ORE OMT AN ect t OC CONU resani e asit een Date of 7 000 Type of Disinfectant 2 2 2 2 2 11 GENESIS RMP Operating Manual Doc 390 840 V3 0 1 A Decontamination Form 2 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 B Pipetting Status The pipetting status consist of 6 code numbers that define the error that occurred Pipetting Status 601 511 2 2 0 LI LJ B Pipetting Status Identification table 6 Warning signifies seriousness of error table 5 Recovery a
51. d to the tip A tolerance for cohesion effects must be taken into account Coefficient of Variation CV A statistical representation of the precision of a test The function standard deviation mean x 100 Command String A combination of user entered parameters according to the rules of the COMMAND SET which instructs the RSP to perform different actions Conditioning Volume The volume of excess liquid which is aspirated together with the liquid to be distributed and immediately discarded usually in the source container before the dispense process starts It serves to create a controlled state of the system Container One copy of a Container Class usually a tube an MTP well or a reagent bottle etc identified by its position in a Rack and or its unique ID See also Class Container Class A set of properties e g diameter height etc of a container for liquids e g a well of an MTP a tube or a bottle independent of its position on the worktable See also Class Control A liquid containing a known concentration of the substance which is to be tested Used to determine high low cutoff limits and or as reference for quality control The properties of the control are well known and stable CU Central Unit The central electronics board of the RSP seated behind the OPTIBOARD The main microprocessor and EPROM are located on this board The CU is the control center for the instrument and all of its optional devices D
52. e GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 September 2000 Glossary Periodic Tip Replacement The disposable tip replacement procedure which is only executed after a certain defined number of Pipetting Cycles See also Standard Tip Replacement Periodic Wash Procedure A wash procedure which is only executed after a defined number of Pipetting Cycles Pipetting Cycle A sequence of Steps which is repeated in identical or closely similar manner Pipetting Mode Describes the main method by which a liquid can be distributed either by Single Pipetting or Multi pipetting Plate Layout Definition of the positions in a Hack into which Samples and Additives etc are to be pipetted PosEval Position Evaluation A software feature which permits the operator to control the X Y Z position of the arm via keyboard arrow cursor keys Position The physical coordinates of the pipetting tip at a given location on the work table itis expressed as X Y and Z mm from the initialization position Precision See Coefficient of Variation CV Pre Dilution A technique in which a liquid e g sample control is first diluted with Additive or System Liquid Some of the resulting mixture is processed further in a Follow Up Distribution The remainder of the mixture is usually discarded Profile A group of tests performed in one Run Rack One unit of a specific Hack class on a Carrier identified by its position the process
53. e chapter 7 2 Save data and exit the TOPS software Figure 9 1 Off Switch 3 Switch off GENESIS RMP Wait until the green LED is dark Figure 9 2 Electrical Connection 4 Unplug mains socket at rear of instrument from powersupply 5 Disconnect the PC from the GENESIS RMP 6 Clean the entire instrument according to the instructions given in chapter 7 9 1 1 Reporting 1 Fill out a copy of the decontamination form and put it with the instrument 2 Enter the shut down into your service and maintenance logbook 390 924 September 2000 GENESIS RMP Operating Manual Doc No 390 840 V3 0 9 Instrument Shut down Storage 9 2 Storage Protect the GENESIS RMP against dust and debris with a cover Recommendation store the instrument in its original packaging Store all manuals and the service and maintenace logbook with the instrument Notice the environmental conditions during instrument storage see paragraph 1 3 5 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 10 10 1 10 2 10 3 September 2000 10 Packing and Transportation Packing and Transportation Unpacking The instrument packaging has been carefully designed to prevent damage to the instrument and parts for normal shipping conditions Store the original packing materials for possible shipments in case an instrument or module needs repairs at the manufactures site Warning The instrument shall be moved by a
54. eded for the PosID to be able to calibrate If the quiet zone is too small the PosID cannot read the barcodes Figure 6 2 Barcode Position on Reagent Trough Edge of barcode label to be even with upper end of groove Groove indicating barcode center Figure 6 3 Microplate Barcode Position Placement of barcode label centred 6 2 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 8 6 Operation Placing Carriers Racks and Containers onto the Worktable 6 2 1 2 Placing Carriers Racks and Containers onto the Worktable Carriers racks and containers are to be placed on the worktable as shown in figure 6 4 Figure 6 4 Placing Carriers Racks and Containers onto the Worktable Legend 1 Barcode label 2 Barcode slot 3 Strip rack Orientation Line 6 2 1 3 Preparing the Pipetting System Before operating the GENESIS RMP perform the daily maintenance procedures as described in Chapter 7 6 2 2 Switching on the GENESIS RMP The GENESIS RMP is switched on via the main power switch see figure 1 1 Wait untill the green light above the main power switch is lit Switch on the PC connected to the GENESIS RMP September 2000 GENESIS RMP Operating Manual Doc No 390 840 V3 0 6 3 6 Operation Introduction 6 2 3 6 2 3 1 Setting
55. eep Well Plate A sort of microplate with a capacity of more than 400 ul of each Well Mainly used for predilution and or for storage of serum Device An addressable component of the RSP or additional option which can communicate with the CU e g arm diluter valve etc 6 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 September 2000 Glossary Destination The Rack holding the Container s into which liquid is dispensed Diluter Precision pump to aspirate and dispense exactly defined volumes of liquid by means of motor driven syringes Distribution One or a sequence of Pipetting Cycles defined together with the appropriate liquid handling parameters Drive The component of the computer housing a disk floppy or hard disk EPROM Erasable Programmable Read Only Memory chip containing firmware programs Error Handling Mode One out of a variety of possible system reactions e g stop instrument and call operator or aspirate at Z max or skip sample etc triggered by an error condition Excess Volume The volume of excess liquid which is aspirated together not separated by an airgap with the liquid to be distributed It is not dispensed anywhere but discarded to waste or a special position after use and serves to minimize dilution of the reagents by the system liquid Firmware The term used to describe the software which is stored in a fixed form on the EPROM Flex Cable A flat cable w
56. ent Precaution Incorrect use of the GENESIS RMP may lead to false test results and may cause exposure of the operator to potentially dangerous compounds EU Conformity Please consult the Conformity Declaration delivered with each unit by TECAN Schweiz AG listing all applicable directives and standards Product Identification The type plate is located at the rear right of the GENESIS RMP GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 e 2 1 Product Description 1 2 Minimal Computer Requirements Hardware Processor with clock rate gt 200MHz 128 MB RAM at least 64MB RAM monitor VGA 17 floppy disk drive 3 5 1 44 MB CD rom drive hard disk with gt 500 MB free space parallel interface printer port and two RS 232 interfaces The PC must comply with the current Safety and EMC requirements for Information Technology Equipment ITE in accordance to the recognized standards EN 60950 UL 1950 EN 55022 EN 50081 1 FCC Part 15 Class A or equivalent Software Windows NT 4 0 with service pack 3 or higher Interface RS 232 use TECAN supplied cable only Installation Set minimal virtual memory to 200 MB 1 3 Instrument specifications 1 3 1 GENESIS RMP Overall Dimensions Figure 1 2 Instrument Dimensions RMP 100 1405 mm 55 3 RMP 150 1780 mm 70 1 93 7 RMP 200 2380 mm lt 30 7 1000 mm 805 mm a 2 5
57. erial Dilution A fraction of the liquid dispensed into a Destination position is re aspirated and then dispensed together with Aaditive or System Liquid into a further destination position This process can be repeated Usually all destination positions filled in this manner are processed later on Setup The implementation of the hardware on an instrument e g tip type number of diluters per channel etc and the assignment of basic settings e g permissible X range of a specific instrument size of installed syringes on a diluter etc This is usually done during the installation of a new instrument Single Pipetting The Pipetting Mode in which an individual aspiration is performed for every destination position See also Multi pipetting Source Rack The Rack holding the Container s out of which liquid is aspirated Standard A liquid containing a defined concentration of the substance to be tested Is used to create a standard curve by which concentration of the analyte in the Samples can be determined The properties of the standard are well known and stable Standard Wash Procedure The wash procedure executed after every single Pipetting Cycle See also Periodic Wash Procedure Step A sub procedure or an element of a Distribution Submerge The distance the tip will travel downward after liquid is detected This parameter is programmed by the operator to avoid aspirating bubbles or debris at the liquid surface GENESIS RMP
58. eys Display Operating Modes Operating Keys Display Operating Modes Introduction For installation and operating instructions refer to chapter 5 and chapter 6 of this manual Warning Signals If errors occur during run TOPS Run Control produces an audio signal to warn the operator If an alarm device is installed the audio signal is accompanied by a light signal Operating Keys The main power switch see figure 4 1 is the only operating key on the GENESIS RMP In cases of emergency it serves as Emergency Off switch The main power switch is located at the instrument s upper right corner A green LED indicates if the instrument is switched on The green LED is dark if the instrument is switched off Figure 4 1 On Off Switch The GENESIS RMP is controlled via the TOPS software package see chapter 6 and the GENESIS RMP TOPS Software Manual 390 837 GENESIS RMP Operating Manual Doc No 390 840 V3 0 4 1 4 Operating Keys Display Operating Modes 4 4 Display The user interface of the TOPS Run Control software see figure 4 2 serves as operational display element for the GENESIS RMP Figure 4 2 TOPS Run Control Display Elements O OO ET T RARE Errors Warnings and Information 0 Instrument Access ToDo Om DRE Topic 1 Aborts the current action a test or the profile 2 Continues a run after instrument access
59. from 50 1 C up to 60 C in 0 1 C increments The heated incubator can be calibrated at 37 C for normal assays and 46 C for special applications Two ceramic heating plates one at the bottom and one at the top of each slot prevent the condensing of any evaporated liquid The evaporation of water in the plates is below 1 during one hour for a plate filled with 200 ul per well at 37 C The door mechanism is identical to the one of the room temperature incubator so the plates are kept dark during incubation The warm up time from room temperature to 37 is less than 10 minutes The powerful electronics shorten the warm up time for each new loaded plate with room temperature liquid by enhancing the heating power with simultaneous prevention of an overheat The temperature gradient over a plate is below 0 5 C Additionally an optional shaking feature of the incubator is available The whole incubator shakes in a linear mode with amplitude of 1mm and an adjustable frequency between 1 and 10 Hz The default value is 8 2 Hz Every slot may be used for different microplates depending on the temperature Therefore it is possible to process more than six plates with one heated incubator only However if you need a higher throughput you may place a second incubator on the left side of the first one GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 4 4 1 4 2 4 3 September 2000 4 Operating K
60. g Manual Doc No 390 840 V3 0 September 2000 TECAN 0 Oration Setting up a Run No Worklist Start Run x Dialog screen Start Run appears e Click Next Press Nest to start the run The run is started See paragraph 6 2 4 September 2000 GENESIS RMP Operating Manual Doc No 390 840 V3 0 6 9 6 Operation TECAN Setting up a Run Worklist 6 2 3 3 Setting up a Run Worklist To set up a new run proceed as follows Start TOPS Run Control by double clicking the Run Control Icon 5 Run Control RunCtrl1 The Run Control main menu appears File Run Instrument Refill Results View Window Options Hel 1 Bene PH eee e Click the Create new run button Bie m Create new and Information instrument Access US Topic ToDo Abort Select Profile x Dialog screen Select Profile appears Select the profile to be run Tosettests on priority select the test and click Priority Click Next gt Selected Profile Elisa Demo Elisa Common Predilution QQ Elisa Multi 1 QA Elisa Multi 2 BQ No Com Pre Dilution Elisa OC Elisa GB ac elisa EAA No Com Pre Dilution QC Elisa QC Incubator lu Tip Decon Sf Tip Decon QQ No ComPre Dilution frh Tin Nanan Priority For tests set on priority the sample d
61. g To Do 5 6 20 Running a Profile Refilling 6 20 Running a Profile Manually Pipetting of Common Pre Dilution 6 21 Running a Profile Handling Errors 6 21 End 6 22 End of Run Introduction 6 22 End of Run Test Error 6 22 End of Run Pipetting Status Report 6 23 End of Run Test Result Files 6 23 Creating Backup 0 6 24 Switching OM he pentes abe deos eh seek 6 24 Malfunction Troubleshooting 6 25 Basic 5 6 25 Liquid 5 6 26 6 27 POOSID TT 6 27 Heated Shaking Incubator 6 27 Room Temperature 6 28 Magis dua edu quel au Eas 6 28 RP 6 28 GENESIS RMP Operating Manual Doc No 390 840 V3 0 TOC iii TABLE OF CONTENTS TOC iv 7 1 7 1 1 7 1 2 7 2 7 2 1 7 2 2 7 2 3 7 2 4 7 3 7 3 1 7 3 2 7 3 3 7 3 4 7 3 5 7 3 6 9 1 8 1 1 9 2 10 10 1 10 2 10 3 1
62. h 511 Not enough liquid for the intended operation in the container 512 Not enough liquid for the intended operation in the container 513 Collision between tip and PosID module avoided position of PosID unknown This error should normally be handled by the software 516 Power fail circuit a device did not receive power normally during initialization not connected correctly 518 Clot limit passed the tip has moved out and detected an exit signal above the clot limit 519 While retracting clot detector on no exit signal detected 520 While retracting clot detector off no exit signal detected 524 ILID pulse error the clot detector registered illegal pulses 525 Tip not fetched the adapter did not succeed to pick up a disposable tip 526 Tip not mounted before going into dispense position the instrument checked and found no disposable tip on the adapter 527 Before picking up a tip the instrument checked and found that a disposable tip B 2 GENESIS RMP Operating Manual Doc No 390 840 V3 0 was already on the adapter tip not correctly ejected September 2000 e B Pipetting Status Table 3 Errors Causes Cause Meaning Code 709 Plunger overload a syringe drive is probably blocked 710 Valve overload a valve cannot turn to the correct position 711 Plunger move not allowed the syringe plunger is not allowed to move unless the valve is in intake or output position 800 Dispense Check Table 4 Errors Reco
63. hich is used as the medium for power data transmission from a board to a moving part Flush The procedure which rinses the total Liquid System with the purpose of removing air pockets or exchanging the System Liquid It is executed only at the beginning or the end of a Distribution Follow Up Distribution A Distribution which uses as source a position filled in a previous distribution Free Dispense Dispensing without the tip touching the liquid Function Mode One out of a variety of ways to execute a certain function e g liquid detection mode on or off Global Liquid A liquid used for several tests It is in a defined position on the worktable GENESIS RMP Operating Manual Doc No 390 840 V3 0 7 Glossary ILID Integrated Liquid Detector Electronic device mounted on the arm The ILID monitors the capacitance between the pipetting tip and the electrical ground worktable It generates a signal when there is a sudden change in capacitance caused by the pipetting tip coming in and out of contact with an ionic solution This signal is used for liquid and clot detection Initialization A term used to describe the calibration of one or more axes of the RSP The axes are offset to a predefined position When the instrument is fully initialized the X Y Z coordinates are 0 0 Z Max The RSP operates using an absolute coordinate system which requires an X Y Z calibration LED The acronym LED stands for Light Emitting
64. his publication may be reproduced without prior written permission GENESIS RMP Operating Manual Doc No 390 840 V3 0 3 Preface 4 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 September 2000 1 1 1 1 1 1 1 2 1 1 3 1 1 4 1 1 5 1 1 6 1 2 1 3 1 3 1 1 3 2 1 3 3 1 3 4 1 3 5 1 3 6 1 3 7 1 4 1 4 1 1 4 2 2 1 2 1 1 2 1 2 2 1 3 2 2 2 2 1 2 2 2 TABLE OF CONTENTS Product Description 1 1 INTFOGUCTION iaces C 1 1 GENESIS RMP Overview 1 1 Reference Documentation 1 2 Intended US6 su ova Ene ea re n 1 2 1 2 EU Conformity 1 2 Product Identification 1 2 Minimal Computer 1 3 Instrument specifications 1 3 GENESIS RMP Overall 1 3 Worktable Dimensions 1 4 Weight Tm 1 4 Supply Connections 1 4 Environmental lt 1 5 1 5 6 1 5 Liquid System 1 5
65. indicated on nM the screen IC ME e To view plate layout select the plate to be viewed by clicking on it and click Plate Layout e Click Next Plate Layout 21 te Global Liquids x Dialog screen Global Liquids appears TIT TIT Place the global liquids in the containers as indicated on the screen Click Next gt Conjugate Grid 22 RM 1 NC Elisa Grid 23 1 12 NC Elisa 1 Grid 23 3 36 NC Elisa 2 Grid 23 5 20 PC Elisa Grid 23 2 12 PC Elisa 1 Grid 23 4 36 PC Elisa 2 Grid 23 6 20 Predilution Diluent Grid 22 RR 1 Dialog screen DiTi appears only if a P4343 4 44 E ARRA RA profile was chosen that uses DiTi s am Select the rack to be defined by clicking on it Type in number of Complete Columns and the number of Tips in incomplete Dii Type DiTi 200 Grid 3 DiTi 1000 96 Grid 9 DiTi 200 96 Column Grid 3 RM DiTi 1000 36 Click Set piri 1000 pem ESSENCE tone DiTi rack is full click Set to ull All Full All racks will be set to full Disposable Tips are always picked from left to right and from rear to front Make sure that partly used DiTi racks are filled up in this direction GENESIS RMP Operatin
66. ing DiTi Cones Diti cone Cone wrench 7 GENESIS RMP Operating Manual Doc No 390 840 V3 0 7 9 7 Maintenance GENESIS RMP Operating Manual Doc No 390 840 V3 0 TECAN September 2000 September 2000 8 Repair Repair Repairs on the GENESIS RMP instrument modules or parts thereof shall only be carried out by TECAN authorized service technicians TEACN will not accept responsibility for any claim resulting from unauthorized repairs Decontamination In order to protect service and repair personnel contaminated instruments or instrument parts must be decontaminated according to standard laboratory regulations Copy and fill in the Decontamination Form and follow the instructions in this form before calling a service technician GENESIS RMP Operating Manual Doc 390 840 V3 0 8 1 8 Repair GENESIS RMP Operating Manual Doc No 390 840 V3 0 TECAN September 2000 e 9 Instrument Shut down Storage 9 Instrument Shut down Storage 9 1 Instrument shut down Warning Depending on the applications run parts of the unit may have been in contact with biohazardous poisonous or even radioactive materials Thoroughly decontaminate all relevant parts If you intend to shut down the instrument for a longer period 1 Empty the liquid system and thoroughly clean and decontaminate all liquid sys tem components se
67. istribution is processed first Identify Worktable x Dialog screen Identify Worktable appears Place all racks and carriers on the worktable as indicated on the screen Close the front access door Click Next gt If for the current run less sample carriers are needed than indicated on the screen place only the number of carriers required for this cack meo run onto the worktable Grid 12 Tube 13 100mm 16 Pos 60913 Wash station Washstation E The GENESIS RMP is initialized The PosID reads all barcodes The Columbus Washer is rinsed Perform the daily maintenance procedures If not all barcodes could be read see paragraph 6 2 3 5 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 e 6 Operation Setting up a Run Worklist Dialog screen Worklist appears EE aed To disable specific samples test will not be performed 1000605 Elisa A Select sample to be disabled by clicking v 1000608 Elisa on it queen Flies of raemes Click Enable Disable Sample 0 Click Elisa Multi 2 20 0 Orders current number of samples to be tested of Orders number of samples including disabled samples To sort click column header
68. n If a test action finishes earlier than planned the TOPS run module can recalculate the time schedule so that the run finishes earlier see figure 6 7 This option is only recommendable if less than 12 plates are processed With more than 12 plates the time needed to re calculate the schedule becomes too long Figure 6 7 Options Re Schedule on Earlier Termination Run Control Demo Elisa Re Schedule on earlier termination x Eie Bun Instrument Refil Results View Window Help g Error Sound Options Haje eit C Warnings and Information Res Option Cancel Vv Allow re scheduling if a test action is earlier finished as planned Do not re schedule on earlier termination if a run contains more than fiz plates 6 2 4 Running a Profile 6 2 4 1 Running a Profile Introduction When a profile is running the TOPS Run Control shows an overview of the status of the run figure 6 8 In the following situations manual interactions may be necessary during a run The running profile contains To Do steps see paragraph 6 2 4 2 Not enough DiTi s were placed onto the worktable see paragraph 6 2 4 3 Manually pipetting of common pre dilution required see paragraph 6 2 4 4 An error occurred during the run see paragraph 6 2 4 5 Figure 6 8 TOPS Run Control Run Status TOPS Run Control Demo Elisa File Bun Inst
69. n Introduction The GENESIS RMP is a computer controlled liquid handling and microplate handling system with integrated barcode reader microplate washer absorbance reader and temperature controlled incubator The operator controls the system via a personal computer equipped with the GENESIS Instrument Software as well as the TOPS Application Software Liquid System The liquid system includes all instrument parts that contain or directly influence the liquid handling The maintenance of liquid system components in proper operating conditions is essential to the pipetting performances of the instrument see chapter 7 Principle of Functioning Figure 3 1 shows the schematic diagram of the instrument liquid system The liquid system comprises the central components for the GENESIS pipetting functionality It transmits the precise movement of the diluter to the tips through the system liquid The system liquid is delivered to the system in a container and is aspirated and distributed within the whole system via tubes valves and connectors The distribution of the system liquid is effected by the movement of the diluter pistons in several pulses and driven by the fast wash pump Figure 3 1 GENESIS RMP Liquid System 1 System liquid container 2 Valve used for empty system liquid container Tubing used for flushing Fast wash pump Pressure relief valve Wik SSY
70. n in the GENESIS RSP uses the CAN Bus Carrier One unit of a Carrier Class identified by its position on the worktable and or its unique barcode Carrier Class A set of properties e g number of racks X Y and Z offsets of an arrangement of one or several Rack classes independent of their work table position See also Class Class A GENESIS software concept describing either a Container Rack or Carrier which may be placed on the Worktable as often as required GENESIS RMP Operating Manual Doc No 390 840 V3 0 5 Glossary Cleaner The well in which a tip is positioned in order to wash both its interior and exterior surfaces by dispensing System Liquid through the tip Clot Detector A program function issuing a message if the difference between the liquid levels measured before and after sample aspiration does not correspond to the calculated difference of level indicating clots attached to the tip The liquid detector works in both directions i e when moving into a liquid and when coming out This dip out or bi directional feature allows to detect aspirated blood clots The principle is simple The software stores the position at which the dip in signal occurred The volume aspirated and the vial diameter is known Therefore the software can calculate the point at which the dip out signal should occur If the dip out signal occurs at a much higher position it can be assumed that a clot is attache
71. nductive with filter 2304 pieces 612 512 Disposable Tips 1000 ul conductive 2304 pieces 612 513 Disposable Tips 1000 ul conductive with filter 2304 pieces 750 141 Liquid system bottle 10 290 338 Waste bottle for system liquid with snap locks 12 2 GENESIS RMP Operating Manual Doc No 390 840 V3 0 July 2000 July 2000 Additional Devices Part No Description 106 001 106 003 750 620 750 661 5mm with snap locks 619 401 diameter 9 mm 36301 36601 30340 Filter 340 nm 30370 Filter 370 nm 30405 Filter 405 nm 30415 Filter 415 nm 30450 Filter 450 nm 30492 Filter 492 nm 30540 Filter 540 nm 30550 Filter 550 nm 30570 Filter 570 nm 30620 Filter 620 nm 30630 Filter 630 nm 30690 Filter 690 nm 30750 Filter 750 nm 750 014 12 Accessoires and Spare Parts Colombus Washer Manifold 8 nozzles Colombus Washer Manifold 16 nozzles Set of 4 wash liquid containers Set of 4 aspirating tubings for Colombus washer outer diameter 5 m aspirating tubing PVC for Colombus washer waste outer Additional Filterslide for Sunrise Reader without filters Additional Filterslide for Sectra Reader without filters Alarm Device FLASHING Light Module 24V AC DC GENESIS RMP Operating Manual Doc No 390 840 V3 0 12 3 12 Accessoires and Spare Parts Carriers Racks Troughs Part No 613 001 619 404 613 012 613 022 613 013 613 015 613 004 613 005 613 014 613 002 613 003 613 020 613 011 613 016 613 017 613010 6130
72. ner The procedure which removes remainders of distributed liquid from the tip inside and outside and the liquid system to prevent carry over It is executed after each or a few pipetting cycles within a distribution and the liquid is discarded into the cleaner position Waste The position in the wash waste carrier into which a tip is placed for washing its interior The system liquid is dispensed through the tip and then into the outer cavity of the wash waste carrier From there waste liquid flows off through the waste tubing to the waste container Well One of the containers in a Microplate Workarea The instrument area which can be accessed by the pipetting tips and the RoMa Worktable Part of the instrument where the carriers are placed for access by the Liquid Handling Arm Worklist A list in which tests and other pertinent information e g status of the individual distribution are assigned to a number of individually identified Samples Often downloaded by a host to the instrument PC the worklist allows to run various tests on a given set of samples so that every individual sample is subjected to only the required tests X Y Z movement The left right X front back Y and up down Z motions of the pipetting tip Z dispense The height of the point of the tip at which liquid is dispensed Z bottom The lowest possible position the tip is allowed to reach During a search liquid command the instrument will search f
73. ng 3 2 Liquid 3 2 Wash Waste 5 3 3 Hardware System Modules 3 3 Liquid Handling Arm 3 3 Robotic Manipulator Arm RoMa 3 4 Positive Identification 3 5 Colombus Microplate Washer 3 5 Sunrise Spectra Microplate 3 5 Room Temperature 3 6 Shaker Heated 3 6 Operating Keys Display Operating Modes 4 1 Introduction 4 1 Warning 5 4 1 Operating KeyS 4 1 Display cakania ae a E ee 4 2 Malfunction Indicators 4 2 Operation 4 3 Introduction 4 3 4 3 WOLFKIISE e Rd doe oce d ade hee d 4 3 Worklist Query Mode with Worklist 4 3 Installation 5 1 GENESIS RMP Operating Manual Doc No 390 840 V3
74. nt Troughs 20 ml for use in 613 019 100 pieces 12 4 GENESIS RMP Operating Manual Doc No 390 840 V3 0 July 2000 e 2 13 Customer Support 13 Customer Support 13 1 Customer Support on the Internet Visit our customer support website at http www tecan cs ch indexl htm Here you find technical information about our products and the timeschedule for training courses 13 2 How to reach us by Telephone TECAN Schweiz AG and its representatives maintain a fully trained staff of technical specialists around the world For any technical questions contact your nearest TECAN representative Switzerland TECAN Schweiz AG Telephone 441 55 254 82 82 Feldbachstrasse 80 Telefax 41 85 254 82 81 CH 8634 Hombrechtikon E Mail hotline tecan com Switzerland Internet http www tecan com Austria TECAN Austria Ges m b H Telephone 43 624 6 8933 Untersbergstrasse 1a Telefax 43 624 67 2770 5082 Gr dig Salzburg E mail tecan a tecan co at France TECAN FRANCE S A Telephone 33 1 30 68 81 50 Parc d Activit s de Pissaloup Telefax 33 1 30 68 98 13 B timent B HERMES II E Mail 113043 3132 compuserve com 27 Rue Edouard Branly 78190 Trappes Germany TECAN Deutschland GmbH Telephone 49 7951 94170 Theodor Storm Strasse 17 Telefax 49 7951 5038 74564 Crailsheim E Mail Info Tecan de Great Britain TECAN UK Ltd Telephone 44 1491 875087 018 The High Street Telefax 44 1491 875432 Goring on Thames E Mail 100751 2354
75. om temperature 5 50 41 F 125 or 50 1 C 60 122 2 F 140 C Temperature Setting Steps 0 1 C 0 1 F Accuracy of Slot temperature 0 5 C at 37 C 0 5 F at 98 6 Gradient over Microplate 0 5 C Shaking Frequency Range 1 8 2 Hz 8 2 Hz is default Amplitude 1mm Frequency Setting Steps 0 1 Hz Supply Voltage Range 24 V 26 4 VDC 1 3 7 4 PosID Laser Class Class 2 Power 0 95 mW max Wave Length 670 nm Readable Barcode Types Code 39 length 0 32 Code 39 full ASCII Codabar length 0 32 Code 128 length 0 32 2 5 Interleaved length 0 30 even only length not setable 12 UPC E length not setable 8 EAN 8 length not setable 8 13 length not setable 13 1 3 7 5 Reader see Operating Manual for SUNRISE Remote Control I 137 301 or Operating Manual for Spectra amp Rainbow Readers I 139 003 1 3 7 6 Washer see Operating Manual for Columbus Washer 1109 004 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 1 4 1 4 1 1 4 2 September 2000 1 Product Description Equipment Standard Delivery A standard delivery follows the specifications on the order configuration sheet Usually it consists of a GENESIS RMP basic instrument including RoMa LiHa with tips washer reader PosID heated incubator RT incubator 1 set of racks and software Accessories and Spare Parts For a list of accessories and spare parts please refe
76. on no exit signal detected after aspiration clot in liquid on the mechanical level Z drive obstructed before dispense while moving down no liquid during dispense syringe drive blocked after dispense no exit signal detected B Pipetting Status Table 2 Pipetting Action Involved Error Error Name 13 Dispense Move in Z 20 Mixing Move Down 21 Mixing only 22 Mixing Retract 23 Mixing Move in Z 30 Flush 31 Wash 32 Pickup Diluent 33 Pickup 34 Put Down 35 Diluter Initialization 36 Move Arm X or Y 37 Move Tip in Z 50 Dispense Check Read Table 3 Errors Causes Meaning the error occurred on the mechanical level Z drive obstructed before mixing while moving down no liquid during mixing syringe drive blocked after mixing no exit signal detected on the mechanical level Z drive obstructed during flushing during washing while picking up diluent while aspirating air etc syringe drive blocked while dispensing diluent air etc syringe drive blocked while initializing the precision pump valve syringe drive blocked while moving arm in X or Z drive blocked while moving the tip up or down Z drive blocked during dispense check with reader Cause Meaning Codes 509 The tip was not able to detect any liquid container is empty or liquid not detect able with the given settings 510 Drives are in a no load state after a cras
77. or liquid from Z start down to Z bottom If the tip reaches Z bottom without finding liquid the instrument reacts according to the liquid detection error mode selected Z start The height of the tip at which the ILID is switched on during a search liquid command It is usually slightly above the rim of the liquid container Z travel The height at which the tip moves from one X Y position to another Moves which cross different racks always use the highest Z travel defined GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 September 2000 1 Product Description Product Description Introduction GENESIS RMP Overview The GENESIS RMP is a Robotic Microplate Processor for ELISA Enzyme Linked Immuno Sorbent Assay and ELISA like tests It is produced in three sizes instrument width 100 150 and 200 cm respectively It is equipped with a Robotic Manipulator Arm RoMa and a Liquid Handling Arm LiHa with 4 or 8 tips Furthermore the GENESIS RMP contains a room temperature incubator a heated incubator a reader a washer a shelf for 4 6 8 or 12 microplates and a barcode reader PosID The access door is secured with doorlocks see figure 1 1 Optional the instrument can be equipped with a second room temperature incubator and or heated incubator The GENESIS RMP is operated via the Test Oriented Plate Scheduler TOPS Run Control software Figure 1 1 GENESIS RMP 150 9 9 3 1 9 9 1 Pi
78. peting tubing 9 Absorbace Reader 2 Diluters 10 Incubator with optional shaker 3 Main power switch 11 Robotic Manipulator arm RoMa 4 Top safely panel 12 Access door 5 Wash bottle rack 13 Worktable 6 Loading Port RT Incubator 14 Positive identification PosID 7 Access door lock 15 Shelf 8 Microplate Washer 16 Liquid Handling arm LiHa GENESIS RMP Operating Manual Doc No 390 840 V3 0 1 1 1 Product Description 1 1 2 Reference Documentation Document Doc No Instrument Operating Manuals GENESIS RMP Operating Manual 390 840 Operating Manual for Columbus Washer 1 109 004 Operating Manual for SUNRISE Remote 1137 301 Control or Operating Manual for Spectra amp Rainbow 1139 003 Readers Application Software Manual GENESIS RMP TOPS Software Manual 390 837 Maintenance Logbook GENESIS Service and Maintenance Logbook 390 924 Instrument Software Manual GENESIS Instrument Software Manual 390 791 Manuals must be stored within reach of the instrument Intended Use The GENESIS RMP Robotic Microplate Processor is intended for fully automated processing of 96 well microplate based ELISA Enzyme Linked Immuno Sorbent Assay and ELISA like tests starting from sample pipetting and ending with result reading The user is responsible for correctly applying the GENESIS RMP according to all local state and federal laws that may apply All precautions must be followed to ensure safe operation of the instrum
79. prime Fill verification during wash Wash liquid on plate support Reader Symptom Lamp low No measurement filter defined No reference filter defined Wavelength nm not available GENESIS RMP Operating Manual Doc No 390 840 V3 0 Room Temperature Incubator Possible Cause Clogging of nozzles Clogging of nozzles Conductivity of wash liquid too low Clogging of nozzles Conductivity of wash liquid too low Droplets on manifold due to splashing Overflow position of aspiration needles too high Manifold leakage Possible Cause The optical system is receiving not enough light Measurement filter was not defined Reference filter was not defined The defined reference or measurement filter is not available on the inserted carriage If the loading port door is not closing properly the magnet may be too far to the back Turn the screw to correct If the slot doors keep open call an authorized Tecan service technician To Do Clean see 7 3 3 2 Clean see 7 3 3 2 Change wash liquid Clean see 7 3 3 2 Change wash liquid conductance must be between 5 mS cm and 30 mS cm Clean and dry manifold If using round bottom microplates reduce dispense speed and or adjust dispense position call authorized Tecan service technician Call authorized Tecan service technician Call authorized Tecan service technician To Do Check lamp If the lamp is working and positioned
80. r to chapter 12 of this document GENESIS RMP Operating Manual Doc No 390 840 V3 0 1 7 1 Product Description GENESIS RMP Operating Manual Doc No 390 840 V3 0 TECAN September 2000 2 1 2 1 1 September 2000 2 Safety Instructions Safety Instructions Introduction Definition Operator Any person who uses the equipment for its intended purpose Service Technician Person authorized by TECAN responsible for installation and initial start up of the instrument Person to carry out service and maintenance task and therefore to be contacted in case of any problems with the system Target Group The GENESIS RMP has been conceived for applications the diagnostic and life science market requiring qualified and authorized laboratory personnel Instrument operation requires thorough knowledge of applications instrument functions and software programs as well as all applicable safety rules and regulations Qualification Training To be qualified for instrument operation you must read and understand the written instructions in this Operating Manual have appropriate training and know the relevant safety rules and regulations Importance of the Safety Instructions This chapter contains general information assuring safe operation of the instrument More specific instructions with regard to safety are given throughout this manual at the respective locations where observation is most important Make sure tha
81. r when changing wash liquids Rinse all channels At the end of each day 7 3 3 1 Waste Empty and clean At the end of each day or 8 7 3 4 1 container when necessary GENESIS RMP Operating Manual Doc No 390 840 V3 0 7 1 7 Maintenance 7 2 3 Routine Maintenance Item Action When How Liquid system Clean Once per week 7 3 1 3 Washer Rinse all channels with Once per month 7 3 3 1 ethanol 70 Unclog nozzles Once per week 7 3 3 2 System liquid Clean Once per week 7 3 4 1 container Wash bottles Clean Once per week 7 3 4 1 Reagent Clean Once per week 7 3 4 1 troughs Fixed tips Replace If damaged or bent 7 3 6 3 RoMa LiHa Clean arm guide Every 6 months or if 7 3 5 1 necessary 7 2 4 Half Yearly and Yearly Maintenance Half yearly and yearly maintenance is to be performed by an authorized TECAN service technician For a list of maintenance tasks to be performed please refer to the GENESIS Service and Maintenance Logbook Doc ID 390 925 Before a TECAN service technician carries out any maintenance work contaminated instruments or instrument parts must be decontaminated according to standard laboratory regulations and the Decontamination Form attached to this manual must be filled in 7 3 Procedures 7 3 1 Liquid System 7 3 1 1 Checking the Liquid System for Leaks The liquid system is leaking if liquid droplets are hanging on the fixed tips or DiTi cones before the GENESIS RMP is switched on If the
82. rcodes The Columbus Washer is rinsed Perform the daily maintenance procedures If not all barcodes could be read see paragraph 6 2 3 5 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 Sp ration Setting up a Run Worklist Query Mode with Worklist Editor C Dialog screen Edit appears pm Select a sample by clicking on it En 2 Selecta test from the Available Tests o window by clicking on it Toone 1 i toe To assign the test to the sample shift the test to the Assigned Tests window by ror i clicking th arrow button 1000703 T 100070 1 1000705 1 1000706 1 100070 1 Ex nen 1 5 Repeat repeats the last assignment Import Last Worklist the last worklist that was used in TOPS Run Control will be imported Itm 3H Dialog sereen Worklist appears Tm ta d dm To disable specific samples test will not be ire ee performed 1000605 Elisa Eae Select sample to be disabled by clicking v 1000508 Elisa on it meee Click Enable Disable Sample Ela 0 Click Next gt Elisa Multi 2 20 0 Samples current number of samples to be tested Rejected Samples number of samples including disabled samples To sort click column header Dialog screen Testkits appears Testkit
83. redilution rack E Pre Dilution Rack ose C serak Select the sample that were manually F pipetted and click Selection manually Sample Pre Dilution Test Pre Dilation Flack Id Dilution Rack Location pipetted or if all samples were manually 1330002 ComPre DW 2 DwDestl Shelf 8 Pos Shelf1 B 1 1330003 ComPre DW 2 DWDestl Shelf 8 Pos Shelfl ca pipetted click All manually pipetted X 1330006 ComPre DW 2 Shelf 8 Shelf 01 3 gt e Click Done 1390011 2 Dw Nest Shelf Pns Shelf A 4 3 Selection manualy pipetted Al manualy pipetted Alarm Off Pia Click Continue The run continues Running a Profile Handling Errors Errors occurring during a run are listed in the Run Control status screen figure 6 8 An audio signal indicates that an error occurred Three types of error messages may occur Error Requires access to the instrument by the user Error cause shall be restored before run can be continued Warning Informs the user about a failure which may soon occur This message has no influence on the run Information to the user No influence on the run To handle the error Double click on the error message in the Run Control status screen figure 6 8 to get more information about the error Handle the error and click continue see figure 6 8
84. rument Refill Results View Window Options Help 81 x Errors Wamings and Information Eri 4 Instrument Access Continue Time Tope JE jElisa 5 Multi 1 Elisa Multi 2 Predilution pre a i ie ee TE ME n 1740 17 50 18 00 18 10 18 20 18 30 18 40 18 50 19 00 1810 1820 1830 For Help press F1 Time 1851 14 1738 September 2000 GENESIS RMP Operating Manual Doc No 390 840 V3 0 6 19 6 Operation Running a Profile Handling To Do Steps 6 2 4 2 A 6 2 4 3 Running a Profile Handling To Do Steps If a profile includes To Do steps these steps are listed in the Run Control status screen figure 6 8 An audio signal indicates that a To Do step must be performed Warning The current Test Profile will not continue until a To Do step is handled If the operator does not handle the To Do step immediately the results will not be reliable Handle To Do steps immediately To Do steps may be Load global liquid Load plate Any other manual interaction defined in the profile To handle To Do steps Click Instrument Access and wait until the status changes to UNLOCKED Follow the instructions given on the screen and click OK Click Continue The run continues Running a Profile Refilling DiTi s To refill DiTi s proceed as follows Click Instrument Access
85. t all Safety Instructions in this publication are strictly followed GENESIS RMP Operating Manual Doc No 390 840 V3 0 2 1 2 Safety Instructions amp 2 2 Warnings Cautions and Notes Three types of informational notices are used in this manual These notices highlight important information or warn the user of a potentially dangerous situation The follow ing notices will have the same level of importance throughout this manual Warning Indicates the possibility of severe personal injury loss of life or equipment damage if instructions are not followed Caution Indicates the possibility of severe personal injury loss of life or equipment N damage if instructions are not followed EP Note Gives helpful information about the equipment 2 2 1 Barcode Scanner PosID Option Laser Class 2 Laser light do not look into the beam Migth be harmful fo your eyes To ensure proper operation the Laser Beam Output Window must always be perfectly clean Even slight soiling will cause reading errors 2 2 2 Use of Mobile Phones C The use of mobile phones in the vicinity of the GENESIS RMP may cause false liquid G detection Mobile phones must be banned from the room in which a GENESIS RMP is 7 installed Turn off mobile phones no stand by operation 2 2 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 3 1 3 2 3 2 1 September 2000 3 Structure and Function Structure and Functio
86. t be changed via TOPS Run Control It is defined by the profile to be run No Worklist In the worklist operation mode no external worklist is available The operator creates a worklist in TOPS Run Control by allocating groups of samples sample batches to the respective tests to be performed Worklist In the worklist operation mode an external worklist is imported in TOPS Run Control The operator can modify the worklist Worklist Query Mode with Worklist Editor In the worklist query mode operation mode no external worklist is available The operator creates a worklist in TOPS Run Control by allocating single samples to the respective tests to be performed GENESIS RMP Operating Manual Doc No 390 840 V3 0 4 3 4 Operating Keys Display Operating Modes 4 4 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 September 2000 5 Installation Installation The initial installation of a GENESIS RMP or an optional module must be carried out by a TECAN authorized service technician according to separate instructions available to service technicians only GENESIS RMP Operating Manual Doc No 390 840 V3 0 5 1 5 Installation 5 2 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 6 1 6 1 1 September 2000 6 Operation Operation Introduction Safety Instructions Carefully read the safety instructions in chapter 2 before operating the GENES
87. that define the error that occurred Pipetting Status 601 511 2 2 0 LI LJ Identification not an error message Warning signifies seriousness of error Recovery action Cause of the error Pipetting action involved Liquid involved If no pipetting error occurred the pipetting status is 0 0 0 0 0 An overview of all codes and their meaning is given in appendix B 6 2 5 4 End of Run Test Result Files The result files created by TOPS can be read during a test or after a run To read the result files created by TOPS proceed as follows File Instrument Refil Window Ti a by double clicking on it View Results Configure Viewer For an explanation on the pipetting status refer to paragraph 6 2 5 3 and appendix B Sort column by clicking on column header Elisa Multi 1 10 20 99 11 08 07 Elisa Multi 2 10 20 99 11 10 39 Elisa 10 20 99 11 13 08 Elisa Multi 2 10 20 99 11 23 39 Elisa Multi 1 10 20 98 11 24 10 Elisa 10 20 99 11 29 41 Common Predilution 10 20 33 14 16 05 Elisa 10 20 99 14 17 34 Elisa Multi 2 10 20 99 14 19 34 Elisa Multi 1 10 20 98 14 20 05 Elisa 10 20 99 14 23 05 4 1 Test Result Files Open Result Evaluation Files September 2000 GENESIS RMP Operating Manual Doc 390 840 V3 0 6 23 S Opmton CC End of Run Test Result Files 6 2 6 Creating Backup folders TOPS automatically prompts the user to
88. tions Hel Click the Create new run button Eoma Create new run and Information nstrument Access Continue ToDo Abort Select Profile x Dialog screen Select Profile appears Select the profile to be run Tosettests on priority select the test and click Priority Click Next Selected Profile Elisa Demo Elisa B B Common Predilution QQ Elisa Multi 1 QQ Elisa Multi 2 EQ No ComPreDilution Elisa QC Elisa GB ac elisa QQ No ComPre Dilution QC Elisa QC Incubator Tip Decon QB Tip Decon EQ No ComPre Dilution Tin Nann Priority For tests set on priority the sample distribution is processed first Identify Worktable x Dialog screen Identify Worktable appears Place all racks and carriers on the worktable as indicated on the screen Close the front access door Click Next If for the current run less sample carriers are needed than indicated on the screen place only the number of carriers required for this lt Back cw run onto the worktable Grid 12 Tube 13 100mm 16 Grid 13 Wash station Washstation x The GENESIS RMP is initialized The PosID reads all barcodes The Columbus Washer is rinsed
89. uthorized TECAN service personnel and with the help of the special lifting devices only Do not attempt to lift the instrument by holding it at arm guides or cover panels The Genesis RMP is a precision instrument Transporting or moving it by the arm guides or covers might cause the latter to get damaged or even break off Serious instrument damage or even injuries may result if this instruc tion is disregarded Packing The GENESIS RMP shall be packed by TECAN authorized service personnel only Use original packing material Transportation All TECAN guarantees are void if the instrument is not correctly packed by TECAN authorized service personnel for shipping Contact your TECAN representative for assistance GENESIS RMP Operating Manual Doc No 390 840 V3 0 10 1 10 Packing and Transportation 10 2 GENESIS RMP Operating Manual Doc No 390 840 V3 0 TECAN September 2000 11 September 2000 11 Disposal Disposal TECAN instruments and parts are made of environmentally inert materials which can all be discarded according to normal waste regulations applicable in the specific country Warning The enesis RMP be contaminated with hazardous materials Thoroughly decontaminate the Genesis RMP before disposal GENESIS RMP Operating Manual Doc 390 840 V3 0 11 1 11 2 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000 12 July 2000 12 Accessoires and Spare Parts Accessoires
90. very Actions Recovery Code 1 a A 10 11 12 20 Meaning After the Error the instrument retried the pipetting action skipped the pipetting action moved to Z bottom aborted the method finished the current pipetting action and then stopped continued with the method with the order to stop when the method is exe cuted ignored the error and attempted to continue as if no error had occurred searched for Disposable Tips retried the Wash cycle disabled the diluter channels went for a new liquid position manually pipetted Table 5 Errors Warning 0 Pipetting was not affected and has been executed 1 the error was not critical for the quality of the results 2 critical error result is not useable Table 6 Errors Identification 0 Identification by Posld or not bar coded 1 User entered the Identification manually September 2000 GENESIS RMP Operating Manual Doc No 390 840 V3 0 B 3 B Pipetting Status ECAN B 4 GENESIS RMP Operating Manual Doc No 390 840 V3 0 September 2000

Download Pdf Manuals

Related Search

Related Contents

Altova SchemaAgent User Manual  Harbor Freight Tools 32206 User's Manual  Manuel d`utilisation PL92FR-HT4 Perceuse à  BUNDLE HANDLER - Ingersoll Rand  Manual - RayBiotech, Inc.  Valueline VLCP34800B30  SMS Smart Media Solutions AE023042 project mount  assembly    

Copyright © All rights reserved. Failed to retrieve file