Fisherbrand II Multichannel User Manual 2002
Contents
1. on a shelf countertop or pipette stand To use hang the grippy finger rest on the shelf hanger PIPETTING TECHNIQUES Push and release the push button slowly at all times particularly when working with high Figures 4 7 viscosity liquids Never allow the push button As Ready position to snap back B First stop Make sure that the tip is firmly attached to the C Second stop tip cone Check for foreign particles in the tip Before you begin your actual pipetting work fill and empty the tip 2 3 times with the solution that you will be pipetting Hold the pipette in an upright position while aspirating liquid The grippy should rest on your index finger Make sure that the tips pipette and solution are at the same temperature FORWARD TECHNIQUE wi Fill a clean reagent reservoir with the liquid to be dispensed 1 Depress the push button to the first stop 2 Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm and slowly release the push button Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess liquid 3 Deliver the liquid by gently depressing the push button to the first stop After a delay of about one second continue to depress the push button all the way to the second stop This action will empty the tip 4 Release the push button to the ready position If necessary change the tip and continue pipetting EJREVERSE TECHNIQ2. grade 3 water conforming ISO 3696 Tests are done in a draft free room at a constant 0 5 C temperature of water pipette and air between 15 C to 30 C The relative humidity must be above 50 Especially with volumes under 50 ul the air humidity should be as high as possible to reduce the effect of evaporation loss Special accessories such as the evaporation trap are recommended PROCEDURE TO CHECK CALIBRATION The pipette is checked with the maximum volume nominal volume and with the minimum volume A new tip is first pre wetted 3 5 times and a series of ten pipettings is done with both volumes A pipette is always adjusted for delivery Ex of the selected volume Procedure 1 Do 10 pipettings with the minimum volume 2 Do 10 pipettings with the maximum volume 3 Calculate the accuracy A and precision cv of both series 4 Compare the results to the limits in the Table 1 If the calculated results are within the selected limits the adjustment of the pipette is correct TABLE 1 Maximum permissible errors according ISO8655 Range Channel Volume Accuracy Precision u u s d ul cv 1 10 ul Sj 12 10 0 24 24 0 16 1 6 1 0 24 24 0 16 16 5 50 ul fs 122 MS 50 10 20 0 4 0 8 5 10 20 0 4 8 0 30 300 ul 8 12 300 80 2 7 3 0 1 0 30 80 26 7 o 10 0 EJADJUSTMENT Adjustment is done with the service tool 1 Place the service tool into the openings of the calibration nut at the top of
3. Remove the piston bar and clean the pistons with a dry nap free cloth Clean the tip cones 0 If needed replace the seal by carefully releasing the cover ring from its snap joint with the screwdriver Remove all the parts from the tip cone Clean all the parts Reassemble the tip cone 14 5 50 ul and 50 300 ul Take one piston Slide cover ring 32 larger hole spring 33 support ring 35 o ring 37 bigger 5 50ul and o ring 36 smaller onto the piston Grease the o ring with the lubricant provided in the pipette package Slide all the parts into the tip cone and close the snap joint of the cover ring gi S 0 5 10 ul Take one piston Slide cover ring 32 larger hole support 35 o ring 36 bigger o ring 37 smaller and o ring support 38 onto the piston Then slide spring 39 spring support 40 sharp edges first and o ring 41 onto the o ring sup port 38 Grease the o rings with the lubricant provided in the pipette package Slide all the parts into the tip cone and close the snap joint of the cover ring 11 Grease cleaned pistons with the lubricant provided in the pipette package 12 Install the piston bar with pistons and tip cones in the cover and close the cover with the four six screws 13 Place the tip ejector and module spring on the neck of the module Press the spring below the tip ejector Close the tip ejector with the tip ejector adapter 14 Use the maintenance key to slide the adapter to wider groove in t
4. n vov standards deviation gt PT V mean volume S i l EE E n number of measurements Standard deviation can be expressed as a relative value CV CV 100 x S V MAINTENANCE When the Fisherbrand Finnpipette Il Multichannell is not in use make sure it is stored in an upright position We recommend a Finnpipette stand for this purpose SHORT TERM CHECKING The pipette should be checked at the beginning of each day for dust and dirt on the outside surfaces of the pipette Particular attention should be paid to the tip cone No other solvents except 70 ethanol should be used to clean the pipette LONG TERM MAINTENANCE If the pipette is used daily it should be checked every three months The servicing procedure starts with the disassembly of the pipette DISASSEMBLING ASSEMBLING MULTICHANNEL PIPETTES 1 Press down the tip ejector mH Insert the maintenance pliers under the tip ejector bar to release the 2 tip ejector i 3 Remove the tip cone module by pressing it with the maintenance pliers ft 4 Press the spring and remove the locking pieces from the groove Remove the spring spring support and o ring Place the maintenance key in the adapter groove and pull off the adapter 6 Pull out the tip ejector adapter Lift the upper end of the tip ejector bar slightly and push it back Lift out the module spring 7 Use a screwdriver to remove the four six screws in the module cover and lift off the cover
5. 0030 1 0030 21 1 0028 1 0029 1 0030 1 0030 1 0031 1 0031 21 1 0030 1 0030 1 0031 1 0031 1 0032 1 0032 22 1 0031 1 0031 1 0032 1 0032 1 0033 1 0033 22 8 1 0032 1 0032 1 0033 1 0033 1 0034 1 0035 28 1 0033 1 0033 1 0034 1 0035 1 0035 1 0036 295 1 0034 1 0035 1 0035 1 0036 1 0036 1 0037 24 1 0035 1 0036 1 0036 1 0037 1 0038 1 0038 24 5 1 0037 1 0037 1 0038 1 0038 1 0039 1 0039 25 1 0038 1 0038 1 039 1 0039 1 0040 1 0041 25 5 1 0039 1 0040 1 0040 1 0041 1 0041 1 0042 26 1 0040 1 0041 1 0042 1 0042 1 0043 1 0043 DO 1 0042 1 0042 1 0043 1 0043 1 0044 1 0045 27 1 0043 1 0044 1 0044 1 0045 1 0045 1 0046 27 8 1 0004 1 0045 1 0046 1 0046 1 0047 1 0047 28 1 0046 1 0046 1 0047 1 0048 1 0048 1 0049 28 5 1 0047 1 0048 1 0048 1 0049 1 0050 1 0050 29 1 0049 1 0049 1 0050 1 0050 1 0051 1 0052 295 1 0050 1 0051 1 0051 1 0052 1 0052 1 0053 30 1 0052 1 0052 1 0053 1 0053 1 0054 1 0055 P Fisher Scientific For customer service call 1 800 766 7000 To fax an order use 1 800 926 1166 To order online www fishersci com 02002 Fisher Scientific Litho in U S A BN 1507920 01
6. 2079108 pcs 42 2207920 12 pcs 8 ch SPARE PARTS Figure 14 lists spare parts and reorder numbers annel 1058180 10593260 1030590 10593750 10593740 2205970 10594560 10594550 1131430 0202040 0202020 1131930 10594570 u 10592510 2207390 1131890 10589520 2205950 1061020 1131400 1131790 10589500 1030480 1030160 2208850 2209080 8 pcs 2209090 12 pes 12 channel 10 1058180 12 10593260 13 1030590 14 10593750 15 10593740 16 2205980 17 10594590 18 10594580 19 1131430 20 0202040 21 0202020 22 1131930 23 10594600 50 300 pl 5 10592520 6 2207460 11 1130720 30 10589160 31 2205960 32 10589490 33 1131400 34 1131790 35 10589510 36 1030140 42 2205270 42 2207080 8 pcs 42 2207090 12 pcs Shelf hanger 2206740 Temperature Air pressure hPA mbar 800 853 907 960 1013 1067 i5 1 0018 1 0018 1 0019 1 0019 1 0020 1 0020 11515 1 0018 1 0018 1 0019 1 0020 1 0020 1 0021 16 1 0019 1 0020 1 0020 1 0021 10021 1 0022 16 5 1 0020 1 0020 1 0021 1 0022 1 0022 1 0023 17 1 0021 1 0021 1 0022 1 0022 1 0023 1 0023 1745 10022 1 0022 1 0023 1 0023 1 0024 1 0024 18 1 0022 1 0023 1 0024 1 0024 1 0025 1 0025 lew 1 0023 1 0024 1 0025 1 0025 1 0026 1 0026 19 1 0024 1 0025 1 0025 1 0026 1 0027 1 0027 195 1 0025 1 0026 1 0026 1 0027 1 0028 1 0028 20 1 0026 1 0027 1 0027 1 0028 1 0029 1 0029 20 5 1 0027 1 0028 1 0028 1 0029 1
7. Fisherbrand 9 Finnpipette ll Multichannel INSTRUCTIONS FOR USE f Fisher Scientific 2000 PARK LANE Pittsburgh PA 15275 800 766 7000 CONTENTS PRODUCT DESCRIPTION PIPETTE OPERATION PIPETTING TECHNIQUES CALIBRATION MAINTENANCE TROUBLE SHOOTING 11 PACKAGE 11 SPARE PARTS 12 13 o0 gt gt PRODUCT DESCRIPTION The Fisherbrand Finnpipette l Multichannel is a manual digital pipette It operates on the air displacement principle i e an air interface and uses detachable disposable tips The adjusted delivery volume is displayed digitally on a readout window on the handle The five different models of Fisherbrand Finnpipette Il Multichannel pipettes cover a volume range from 0 5 ul to 300 ul Fisher Catalog Number Volume Range Tip Size 21377825 8 channel 0 5 10 ul micro 10 2 877827 8 channel 5 50 ul 250 300 21377829 8 channel 50 300 ul 250 300 21377826 12 channel 0 5 10 ul micro 10 21377828 12 channel 5 50 ul 250 300 21377830 12 channel 50 300 ul 250 300 Dera DISPLAY The adjusted delivery volume is clearly indicated in the large digital display on the handle RAW MATERIALS The Fisherbrand Finnpipette Il Multichannel is made of mechanically durable and chemically resistant materials PIPETTE OPERATION SETTING THE DELIVERY VOLUME 2 Set the delivery
8. UE The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency to foam easily The technique is also recommended for dispensing very small volumes Fill a clean reagent reservoir with the liquid to be dispensed 1 2 4 Depress the push button all the way to the second stop Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm and slowly release the push button This action will fill the tip Withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess liquid Deliver the preset volume by gently depressing the push button to the first stop Hold the push button at the first stop Some liquid will remain in the tip and this should not be included in the delivery The remaining liquid should either be discarded with the tip or pipetted back into the container REPETITIVE TECHNIQUE The repetitive technique offers a rapid and simple procedure for repeated delivery of the same volume Fill a clean reagent reservoir with the liquid to be dispensed T Depress the push button all the way to the second stop 2 Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm and slowly release the push button This action will fill the tip Withdraw the tip from the liquid touching against the edge of the reservoir to remove excess liquid 3 Deliver the preset volume by gently depressing the push bu
9. he module neck 15 Slide the o ring spring support and spring onto the piston bar and lock with the locking pieces 16 Attach the tip cone module to the handle and the tip ejector adapter to the tip ejector bar SERVICE INSTRUCTIONS FOR MULTICHANNEL PIPETTE TIP CONES To ensure even performance between all channels in a multichannel pipette all tip cones have to be changed at the same time if any of them needs to be changed Don t mix tip cones of different packages because one bag contains a matched set of tip cones CAUTION The Fisherbrand Finnpipette II Multichannell is designed to allow easy in lab service If you would prefer to have us or your local representative service your pipette please make sure that the pipette has been decontaminated before you send it to us Please note that the postal authorities in your country may prohibit or restrict the shipment of contaminated material by mail TROUBLE SHOOTING The table below lists possible problems and their solutions Defect Possible reason Solution Leakage Tip incorrectly attached Attach firmly Foreign particles Clean tip cones attach between tip and tip cone new tips Foreign particles between Clean and grease O ring the piston the O ring and the and cylinder cylinder Insufficient amount of Grease accordingly grease on cylinder and O ring O ring damaged Change the O ring Inaccurate Incorrect operation Follow instructions dispensing ca
10. refully Tip incorrectly attached Attach firmly Calibration altered caused Recalibrate according by misuse for example to instructions Inaccurate Unsuitable calibration Recalibrate with dispensing High viscosity liquids may the liquids in question with certain require recalibration liquids PACKAGE The Fisherbrand Finnpipette Il Multichannell is shipped in a specially designed package containing the following items The Finnpipette Il Service tool Maintenance pliers Tube of grease Instruction manual Calibration certificate Shelf hanger Two stickers CoL e OU EOS T9 c 12 channel 10 8g B E 3 e X O ske ka LJ TOHI 8 channel 10 8g PIT HE e wu et 0 cv 9 Ge SE ce ve w am oT Le I 00 08 Ga 3 pr 0 9 LE Ge ee CE ve E Le Qm O 00 Irlos s cv c Q Q Q Q O m gt sles Qo ost au 0 O o e m0 0 ll Cv 8 irl oL s 0 13 All 1 10593720 2 2900580 3 10593050 4 1527200 0 5 10 pl 5 10592500 6 2207370 11 1131890 24 10593230 30 10593240 31 2205860 32 10589490 33 10593510 34 1131790 35 10593280 36 1030380 37 1030060 38 10593290 39 1131900 40 10593360 41 1030170 42 2205870 42 2
11. the handle 2 Turn the service tool clockwise to increase or counterclockwise to decrease the volume 3 After adjustment check the calibration according to the instructions above FORMULAS FOR CALCULATING RESULTS Conversion of mass to volume V w e xZ V volume ul w weight mg e evaporation loss mg Z conversion factor for mg ul conversion Evaporation loss can be significant with low volumes To determine mass loss dispense water to the weighing vessel note the reading and start a stopwatch See how much the reading decreases during 30 seconds e g 6 mg 0 2 mg s Compare this to the pipetting time from taring to reading Typically pipetting time might be 10 seconds and the mass loss is 2 mg 10 s x 0 2 mg s in this example If an evaporation trap or lid on the vessel is used the correction of evaporation is usually unnecessary The factor Z is for converting the weight of the water to volume at test temperature and pressure A typical value is 1 0032 ul mg at 22 C and 95 kPa See the conversion table on page 15 Accuracy systematic error Accuracy is the difference between the dispensed volume and the selected volume of a pipette A V V A accuracy V mean volume V nominal volume Accuracy can be expressed as a relative value A 100 x A V Precision random error Precision refers to the repeatability of the pipettings It is expressed as standard deviation s or coefficient of variation cv
12. tton to the first stop Hold the push button at the first stop Some liquid will remain in the tip and this should not be included in the delivery 4 Continue pipetting by repeating steps 2 and 3 EIBIPETTING WHOLE BLOOD deproteinization in blood glucose determination for example Use steps 1 and 2 of the forward technique to fill the tip with blood Wipe the tip carefully with a dry clean tissue T 2 3 Immerse the tip into the reagent and depress the push button to the first stop making sure the tip is well below the surface Release the push button slowly to the ready position This will fill the tip Keep the tip in the solution Depress the push button to the first stop and release slowly Keep repeating this procedure until the interior wall of the tip is clear Finally depress the push button all the way to the second stop to completely empty the tip CALIBRATION All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with distilled or deionized water The pipettes are constructed to permit re adjustment for liquids of different temperature and viscosity DEVICE REOUIREMENTS AND TEST CONDITIONS An analytical balance must be used The scale graduation value of the balance should be chosen according to the selected test volume of the pipette Volume range readable graduation under 10 ul 0 00 1 mg 10 100 ul 0 01 mg above 100 ul 0 1 mg Test liquid Water distilled or deionized
13. volume using the push button on the top of the pipette To increase the delivery volume turn the push button counterclockwise To decrease the delivery volume turn it clockwise 2 Make sure that the desired delivery volume clicks into place and that the digits are completely visible in the display window _ Do not set volumes outside the pipette s specified volume range Using excessive force to turn the push button outside the range may jam the mechanism and eventually damage the pipette EMP EJECTION To help eliminate the risk of contamination each pipette is fitted with a tip ejector system The tip ejector system consists of a soft touch tip ejector and specially designed gearing mechanism To release the tip point the pipette at suitable waste receptacle and press the tip ejector with your thumb SAFETY LABEL You can mark the pipette application your initials the calibration date etc on the safety label Remove the clear plastic window on the edge closest to the push button use the service tool that comes with the pipette or a screwdriver Mark the adhesive label with a felt tipped or other pen and snap the window back in place SHELF HANGER You can attach the pipette shelf hanger on a counter pipette stand or anywhere where you want to hang your pipette Clean the area where you plan to attach the shelf hanger Apply two stickers to the underside of the shelf hanger Press the shelf hanger firmly into place
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