Approach to Instrumentation
Contents
1. A 10 Press START will blink until set values are reached 1 Turn ON MasterSwitch 2 Depress foot pedal to release door and install rotor Page 11 pH Meter VWR Symphony Automatic Read Mode Digital Example 3 System should start in Automatic Read Mode with AR blinking Remove probe and insert in watatats your sample OPI Wait until the AR and small Current 1 GR 00S pH display stop blinking Temperature of This is the Measured pH sample gt Rinse probe with DI water and return to stand 1 Press and hold for 2 sec to turn unit on off Press once if needed to go to Automatic Read Mode 2 Press bottom of key to turn on or off lighted display P 12 STLCC CPLS Morrison 8 25 2014 age Genetic Analyzer ABI Maintenance Calibration and Supplies PC Driven Instrument i p apode Buffer 3500 Series PN 4393927 ULN 12345 Exp 31 Dec 2009 1 fen Resewrn Wee Only Wes tar Me Gognoutic naai re Serom2 Cwarc Ponte City CA asi Aado ia USA Sev SPW lene 1ra 7 Supplies Link to ABI 3500 User Manual pdf 18mb Prepared by Bob Morrison STLCC CPLS Instrumentation Specialist STLCC_CPLS Morrison 8 25 2014 Initiated Sep 10 Last Update Jan 2012 Page 13 Genetic Analyzer Genetic Analyzer ABI PC example Fs fog Ex E 3500 Data Collection Software Library Maintenance Tools Manage v Preferences Help Log Out2. Approach to Instrumentation Prepared by Bob Morrison STLCC CPLS Instrumentation Specialist Original April 2008 Revised Aug 2014 STLCC_CPLS Morrison 8 25 2014 Page 1 Approach to Instrumentation Guidelines e Review SOPs Standard Operating Procedures e Safety Issues e Analog vs Digital vs PC e Get help STLCC_CPLS Morrison 8 25 2014 Page 2 Approach to Instrumentation SOPs e Review SOPs Standard Operating Procedures or other materials Visit Users website before you try to use the instrument Jump to a specific instrument from the title page by detecting on the pictures Use Search option if you don t see what you are looking for Review the pictorial SOPs and any Quick Start Guides Note hotlinks that will take you to online copies of manuals and other information e Get help lf this is your first time using the instrument seek help from others who may have experience with the instrument instructor staff classmates Use onboard HELP options or quick start guides Return to SOPs and investigate other pages and or online user manuals STLCC_CPLS Morrison 8 25 2014 Page 3 STLCC CPLS Website Equipment SOPs Protocols Training News http users sticc edu departments fvbio 00 00 4 ee Center for Plant and Life X B St Louis Community College Gi Home A Feeds J ReadMail Prnt Page gt Safety Pi ank and Life Sciences Su Laas Laniiiaty y College
3. St Louis Community College STLCC Center for Plant and Life Sciences CPLS Join us on facebook Search for Stlcc fv Biotech or FVBiotech Instrumentation Description SOPs Protocols Education How to Lab Manuals Training CPLS Brochure CPLS Facility Layout Labs Instrumentation CPLS Organization CPLS Class Schedules Biotechnology Program Bridge to STEM Life Science Program B2S5L5 Chemical Technology Program Clincical Lab Program Contract Research Organization Bio Bench Projects Horticulture Program Mobile Technology Center o News Visitors Press Releases Job Info oO Oo E e 69 60 6 6 60060 60 60 60 6 MJ Research PTC 220 BioRad Model PTC 1148 MJ Research Model PTC 150 a tig Thermal Cyclers PCR qPCR Standard Operating Procedures Select Picture to Jump to that Section device Stratagene qPCR Mx3005P Prepared by Bob Morrison FVCC Instrumentation Specialist June 2008 Latest Revision Oct 2011 HYBAID PCR Omn E STLCC_CPLS Morrison 8 25 2014 Page 5 Approach to Instrumentation Guidelines Safety Look for safety guidelines regarding chemicals or electrical handling before you turn it on If the on button is not obvious trace power cord to the instrument and on off toggle is often nearby Also look for or 0 1 symbol Check Ready on green lights or any warning red amber lights before proceeding Don t force anything pushing o
4. Dashboard Edit Y 4 Maintenance Fj 5 Print v amp History View Calibrate Calibration Settings ring Settings Spatial Q CRL Pass Fail Threshold Spectral Chemistyseefe General Sequencing v Read Length Start umber of Wells 96 96 FastTube 384 Plate Position A se ee Read Length End Keep Spectral Calibration Data 0 rj Performance Check Fragment Install Standard HID Install Standard o Maintenance Wizards Planned Maintenance v Capillary Run Data Capillary Median SD Spectral Calibration Run Contiguous Read Length CRL p s Ref Sequence 4 Check this box to Keep save a Sequenced and Calibration Data Main Wi fh CRL Basepair Accuracy Basepair Accuracy Read Length Notifications Log wi Passed ii Failed Borrowed Not Calibrated Quality Value Condition Status Message v Intensity vs Scan Number Calibrated Data Bin He 0 4000 8000 16000 20000 24000 28000 32000 12000 Intensity vs Scan Number v Sequence Comparison to Sample Capillary 1 Base Position Reference Sample v Intensity vs Pixel Number Capillary 1 B KAKOAK 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 Intensity vs Pixel Number Page 14 STLCC_CPLS_ Morrison 8 25 2014 Approach to Instrumentation Guidelines Review e Review SOPs Standard Operating Procedures o
5. OOK for News on Plant and Life Sciences Search for FVBiotech or Sticc fv Biotech S toui Commenity College at FV Biotechnology Program x Comer for Plat and Life Sciences BRDG Park Facility Pi amA Lhe Saana e beet ee te Ga Wall E Info Photos 4 E Notes Af Friends R Subscriptions Friends 139 Soumya Mallikarjunaiah Nour Al hiyari Giselle Mungal Hornaday Tarak Patel UMSL 4 Sticc fv Biotech d Lives in Saint Louis Missouri amp Add where you work Add your school Edit Profile E Update Status fg Add Photo Video What s on your mind Z Em Sticc fv Biotech a Nov 2011 fi Like Comment Share Novembe E FA Sticc fv Biotech Sa iy m e z a ct T A g 3 Q a m A m 5 i D oO a Sticc fv Biotech Find Friends Home v Edit Profile View As People You May Know See All David Willis 3 mutual friends del Add Friend Jennifer Florida 2 mutual friends dsl Add Friend Brian L Baker 2 mutual friends del Add Friend 2 Megan Wurst 2 mutual friends tel Add Friend Sponsored See All Friends of Clowder House Please help kitties in the St Louis area by liking our page g Like 263 people like this Grab Your L Chat Offline x b
6. r other materials Visit Users website before you try to use the instrument Jump to a specific instrument from the title page by detecting on the pictures Use Search option if you don t see what you are looking for Review the pictorial SOPs and any Quick Start Guides Note hotlinks that will take you to online copies of manuals and other information e Get help Seek help from others who may have experience with the instrument instructor staff classmates Use onboard HELP options or quick start guides Return to SOPs and investigate other pages and or online user manuals e Safety Issues Look for safety guidelines regarding chemicals or electrical handling before you turn it on Ifthe on button is not obvious trace power cord to the instrument and on off toggle is often nearby Dont force knobs dials or press harder on screen menu as this may harm you and the instrument Look for Ready on green lights or any warning lights before proceeding vs Digital vs PC Analog Dials to set and then generally a Start or Run button follow order in SOP to avoid problems Digital Small screen with arrows to navigate like your cellphone not always a back or return option Avoid SETUP menus unless the SOP directs you to change or set them PC More complex instruments use PC application logon with D password select Icon follow menus STLCC_CPLS Morrison 8 25 2014 Page 15 facebook Join Us on FACEB
7. r twisting harder on knobs switches or screen menus is not the answer and may damage the instrument or cause shock STLCC_CPLS Morrison 8 25 2014 Page 6 Electrophoresis Ty Red Electrical Probe leads to far or receiving end of plate If lose or pops out expose metallic end and expand thin strips Black Electrical Probe leads to well or start end of plate Safety Notes 1 Connect all wiring and probes before turning the unit on 2 Never touch the probes or power supply box while the device is on Turn off the device before disconnecting electrical probes Do not handle the device with wet hands or on wet countertop STLCC_CPLS Morrison 8 25 2014 pical Gel Setup Safety example Voltage or Amps dial control Typically set to maintain 95 volts during run Digital Volt or Amp readout Window Rocker Switch Set to constant Volts or Amps per lab procedure Typically 95 Volts DC Page 7 10 11 Electrophoresis E gel Basic Operations SOP and Safety example Before putting an E gel cartridge in the powerbase attach the circular end plug of the power adapter cord into the hole in the base and then plug the adapter itself into the 110V outlet A brief self test of flashing lights and beeping will occur then no lights until step 2 is completed Insert the E gel cartridge into the base right side first then press the left side down There should be an click sound as the ca
8. rtridge snaps into place and a steady red light will appear Ready Mode No button pushes are required to reach this state A two minute pre run with the E Gel comb in place is required before you will load samples into the wells Press and hold either the 15 or 30 min button for a few seconds as the steady red light turns into a flashing green light for the 2 minute run At the end of the pre run the flashing green light will turn to flashing red and a rapid beep will sound Press and release either button to stop the beeps and return to a steady red Remove the comb from the E gel cartridge carefully lifting straight out and then clear up any gel residue near the wells Prepare and load your samples into the wells per the E gel manual guidelines For a single comb gel press the 15 min button a steady blue light will appear for the duration of the run For a double comb gel press the 30 min button a steady green light will appear You may interrupt the run at any time by pressing either button once and then again to restart but you must manually time the remaining portion to avoid overrunning the gel At the end of the run periods the steady light will return to a flashing red light and beeps will sound Press either button to stop the beeping and return the light to a steady red Remove the E gel carefully from the powerbase and you are now ready for the transilluminator and or other analysis Bands will diffuse within 20 minute
9. s however STLCC_CPLS Morrison 8 25 2014 Page 8 Thermal Cycler MyCycler Basic Operations Power example Plug in the unit to provide power there is no toggle on off switch Turn on by pressing the Stand by key once To put into Stand by mode conserving power hold down for gt 3 sec Turn off completely by unplugging the unit there is no on off switch STOP key will pause a protocol when running Pressing STOP again will terminate the protocol HELP menus are available From the HOME startup screen select F5 Help to review topics and display an index of features Hot link to Bio Rad MyCycler User Manual 45 pgs pdf STLCC_CPLS Morrison 8 25 2014 Page 9 Approach to Instrumentation Guidelines e Analog vs Digital vs PC Analog Dials to set and then generally a Start or Run button follow order in SOP to avoid problems Digital Small screen with arrows to navigate like a cellohone not always a back or return option Avoid SETUP menus unless the SOP directs you to change or set them PC More complex instruments use PC application logon with ID password select Icon follow menus STLCC_CPLS Morrison 8 25 2014 Page 10 Actual readings Centrifuge Floor Avanti J 20XP Control Panel BRDG Analog example 5 Set Speed 6 Set Time hr min 4 Press SET press 3 Set dial to match installed rotor i 7 Set Temp C again to see
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