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ScanDrop Nano-volume Spectrophotometer

1. Analytical methods can be selected via tabs 1 8 and 9 16 of the sample setup area 1 Define reference sample 2 Activate Expr key 3 Select analytical formula from list field A selected formula can be edited working in the Default settings dialog screen refer to section Analytical Parameter Presettings Programming of Analytical Formula p 62 4 Mark all those sample positions in the sample setup area which you want analyzed according to the selected formula Different formulas can be applied for analysis of different sample locations of a CHIPCUVETTE as part of a measurement process However only an identical formula can be set for the deep test point and the flat test point of a given sample channel or only one of the two test points can be activated Defining sample properties The Sample Prop tab displays sample names and analytical formulas in a table ScanDrop 06 2009 57 Expression Module Settings for amp Management of Analytical Parameters Module Bio 1 8 9 16 Sample Prop Settings Load sample names from file csv Reference e Sample 1 Absorbance 280 Factor 1 38 B Sample 2 Absorbance 280 Factor 1 38 a Sample 3 Absorbance 280 Factor 1 38 s Sample 4 Absorbance 280 e Sample 5 Absorbance 280 New Load Save Fig 26 Expression module Chip definition or modification of sample table 1 To enter or change sample properties pr
2. UV VIS Module 8 5 8 5 1 User Defined Measurement UV VIS Module Working in UV VIS module one may measure samples for transmittance absorbance or energy Available result options are spectrums values obtained at discrete wavelengths or values for two wavelengths or the corresponding differential amount Furthermore multiple measurement Sequences can be performed for kinetic analysis However no optimal measuring range can be determined in contrast to e g operation in Quant module Triggering UV VIS module 1 For ScanDrop 250 Select CHIPCUVETTE tube type in main window 2 Press UV VIS key The UV VIS module window opens Function elements in UV VIS module window The three tabs Chart Table and Settings are available options for presentation of results They are explained in more detail in section Display amp Management of Measured Results p 70 The module s function keys are located at the bottom bar of the dialog window Function Description Para Opens parameter window for measurement parameter settings Open Opens a previously saved result file Save Saves current measurement results Export Exports measurement results Start Triggers measurement with specified parameter settings Eject Moves holder frame out for insertion or removal of a CHIPCUVETTE Close Closes UVVIS module Setting amp Managing Measurement Parameters Measurement parameter settings can be made
3. Content 4 06 2009 ScanDrop General Advice 1 General Advice 1 1 Working with the ScanDrop The ScanDrop provides a UVVIS spectrophotometer that has been specifically designed to perform analysis of micro liter samples with a minimum sample volume of 0 3 uL The ScanDrop is available in three product versions e ScanDrop 100 e ScanDrop 200 e ScanDrop 250 Version 200 Scandrops are intended for measurement of micro liter samples in a patented CHIPCUVETTE type tube ScanDrop 250 systems include an extra tube chimney for insertion of standard tubes They can be used to analyze samples ina CHIPCUVETTE or to measure samples in standard tubes with a layer thickness of 10 mm The ScanDrop 100 is for measurement of samples in a standard tube The optical beam train of all ScanDrop versions integrates a xenon flash lamp serving as excitation light source and a diode array with a holographic grid as the receiver part Because there are no mechanically moving parts inside the polychromator the ScanDrop performs with exceptional stability yet providing high wavelength accuracy at the same time Configured with a combination of a xenon flash lamp and a split beam component for reference gauging the system will work in a stable state and be ready for measurement immediately on turning power on No run in time is required to achieve consistent test readings The ScanDrop is prepared for measurement within a spectral range from 190
4. Cycles tab Select single measurement or cyclic multiple measurement The results of a cyclic multiple measurement process e g a series of spectrums are saved in a single cyclic file Option Description No Selects single measuremnt normal mode Automatic Automatically repeats measurement of a sample Specifies a number of desired repeat measurement at the Number input field Time controlled Selects time controlled repetition of a measurement for kinetics Once active this option opens a table for defining a maximum of four subphases intervals with different interval times Waiting time Defines the length of time by which the actual start of measurement is delayed against the starting command This time specification can be used as incubation time for kinetic jobs Time intervals s Defines total length of a subphase Time s Time interval between two successive measurements within a given subphase Start request Actual measurement will not start before the Trigger measurement query has been acknowledged when this ScanDrop 06 2009 67 User Defined Measurement UV VIS Module Setting amp Managing Measurement Parameters option is active Kinetic slow Performs kinetic fast measurement if disabled Launches kinetic slow measurement if enabled Module UY YIS Parameter General Meas mode Cycls l malonil C No Automatic Time controlled JV Start request T Slow k
5. Water Take care to prevent penetration of liquid into the inner working space Do not place liquid containing vessels onto the ScanDrop Otherwise the system may suffer damage Danger of corrosion Do not operate the ScanDrop in the direct vicinity of aggressive vapors for example strongly etching acidic or alkali fumes Such fumes might corrode terminals mechanical and optical parts Keep the sample compartment free from exposure to strongly etching substances in order to prevent adverse influences Use vapor tight tubes with a plug in such cases Touch screen scratching Use only your finger tips or a suitably chamfered input aid of plastic material palm pen to make entries at the touchscreen Refrain from the use of sharp edged objects or pins 06 2009 ScanDrop Safety Instructions Standards amp Directives 2 2 Standards amp Directives Protection class and degree of internal protection The system casing is designed to comply with internal protection standard IP 20 Operating safety The ScanDrop is designed and built to meet the following safety standards e DIN EN 61010 1 VDE 0411T 1 IEC 61010 1 EMC compatibility The ScanDrop has been tested for radio interference suppression and noise immunity and found to fulfill the requirements according to e DIN EN 61326 1 Environmental compatibility The ScanDrop has been tested for environmental compatibility and found to meet the requirements according to
6. Note To save a big file for example the result of spectrum measurement at all test points of a CHIPCUVETTE takes several seconds The screen keys remain disabled until saving is complete Measured results are saved in the UV VIS module window 1 Press Save key in results window The standard window for file saving opens 2 Select path and file name and click OK for saving ScanDrop 06 2009 71 User Defined Measurement UV VIS Module Display amp Management of Measured Results Data can be exported into a text file where they will be available for further processing in other programs for example Excel Data exports can be triggered in the UV VIS module window 1 Press Export key in results window The standard window for file saving opens 2 Select path and file name and press OK to save a fiven file 72 06 2009 ScanDrop Quantitative Analysis Quantification Module Measurement in Standard Tube Quantitative Analysis Quantification Module The Quantification module is mainly intended for express jobs of routine concentration analysis A concentration value is determined working from the absorbance at a given wavelength Concentration analysis may rely on a factor or a linear or square regression module alternatively A routine procedure processes predefined sequences of reference samples standard samples and samples for concentration or absorbance measurement Working in
7. A 260nm 40 Absorbance 260 nm factor 50 x ug ml A 260nm 50 Absorbance 280 nm x A 280nm Absorbance 280 nm factor x mg ml A 280 nm 1 38 1 38 Kalb Bernlor x ug ml A 230nm 183 A 280nm 75 8 Kalckar Shafran x mg ml A 280nm 1 45 A 260nm 0 74 Warburg Christian DNA x mg ml A 260nm 62 9 A 280nm 36 Warburg Christian Protein x mg ml A 280nm 1 55 A 260nm 0 76 Whitaker Granum x mg ml A 235nm A 280nm 2 51 On selection of a method the absorbance values which are required for a given analytical process will be determined at the appropriate wavelengths and the results be calculated and displayed Automatic determination of optimal measuring range Similar to the procedure followed in Quantification module a measurement outputs two measured values that have been determined for both test points of a sample channel Of the two measured values that one will be included in analysis which is within a linear measuring range of 0 005 to 1 5 Abs If both measured absorbance readings are within this range the one which is closer to 0 75 Abs will be selected Measured values are checked for accurary according to these criteria e Abs 1mm gt Abs 0 1 mm e Abs 1mm gt 0 005 e Abs 0 1 mm lt 1 5 Abs 1 mm Absorbance value for 1 mm layer thickness Abs 0 1 mm Absorbance value for 0 1 mm layer thickness 46 0
8. Meas Sample measurement Results will be output in parameters as defined via General tab and Meas mode tab No Deletes individual sample positions AII Assigns above selected sample type to all sample channels 68 06 2009 ScanDrop User Defined Measurement UV VIS Module Setting amp Managing Measurement Parameters Module UY YIS Parameter Cancel Fig 33 UVVIS module Chip parameter settings with sample sequence 8 5 1 2 Saving amp Loading Measurement Parameters For management of measurement parameters contained in the UVVIS module the two keys Open and Save are provided in the module s parameter window The default path for file saving is in UVVIS module is StorageCard ScanDrop UVVIS The file extension is par Files must always be saved on the storage card Storage Card folder The memory space of the HID Pro 320 operation control unit is not big enough for this purpose Saving a measurement parameter file 1 Specify measurement parameter settings you want saved when working in the parameter window 2 Press Save key The standard window for file saving opens 3 Select path and file name and press OK for acknowledgement Loading analytical parameters Analytical parameters are saved in the same way as measured results A parameter file always contains analytical parameters A result file contains the absorbance readings for the measured samples in addition
9. Settings Show Sample 2 m Both channels Fig 34 UVVIS module Chip graphic representation of spectrum Table tab The Table tab contains a table of samples with an overview of measured results 70 06 2009 ScanDrop User Defined Measurement UV VIS Module Display amp Management of Measured Results Chart Table Settings 1 6781 1 6908 1 6172 0 2023 1 6029 1 555 0 1889 1 5399 1 5018 0 1789 1 4867 1 4547 0 1714 1 4398 1 4103 0 165 1 396 gt Para Open Save Export Start Eject Close Fig 35 UVVIS module Chip samples table Settings tab Displays the following measurement parameter data and saves them together with the measured results Meas mode Spectral range or wavelengths being measured Display Shows magnitude of ordinate of results of measurement while measurement is going on Accumulation Number of repetitions of a single measurement Interval Measurement to measurement interval in cyclic measurement mode Opening saving and exporting measured results The default path for file saving in UVVIS module is StorageCard ScanDrop UVVIS The file extension is dac Files with previously saved results of measurement can be opened in the UV VIS module window 1 Press Open key in parameter window The standard window for file opening opens 2 Select dac file with analytical results from UV VIS module and click OK to open
10. cbi file with analytical results of a prior bio method and press OK for acknowledgement Measured results can be saved in the results window 1 Press Save key in results window The standard window for file saving opens 2 Select path and file name and press OK for saving Data can be exported to a text file where they will be available for further processing in other programs for example Excel For data exporting the results window is available 1 Press Export key in results window The standard window for file saving opens 2 Select path and file name and press OK for saving ScanDrop 06 2009 53 Bio Method Module Presettings in Bio Module Closing the results window The results window can be exited by pressing the New or the Close control button After a query if unsaved data are to be saved the Bio module parameter window is restored New Parameter window displays basic setings or presettings Close Parameter window preserves the settings of a preceding measurement They can be used for next measurement or be modified 8 3 4 Presettings in Bio Module Presetttings can be made for analytical parameters These will be loaded when the Bio method module starts 1 Press Default key in Bio module window 2 Available parameter setting options Sample type Presets sample type in CHIPCUVETTE sample setup Method Selects a bio method Accumulations Defines the number of repeat
11. details can be opened Position Sample name Type Date Time Result Result Corr 320 nm Table Position on CHIPCUVETTE Sample name Analytical method Time of analysis Analytical result Analytical result after background correction Values measured for absorbance For a CHIPCUVETTE both readings i e one for 0 1 mm and one for 1 mm deep test point are indicated 52 06 2009 ScanDrop Bio Method Module Display amp Management of Analytical Results Module Bio Sample result Position 2 Sample name Type Absorbance 280 Factor 1 38 Date Time 04 04 2122 7 33 Result Measurement values 1 7117 Used channel 1 mm Result Corr 320mm 1 6883 Used channel 1 mm 0 0005 0 0169 0 0 Fig 23 Bio module Chip displays result of a single sample Settings tab The Settings tab displays the Title the Operator the number of Accmulations and the Unit of an analytical procedure These values are saved together with the measured results Opening saving and exporting measured results The default path for file saving in Bio method module is StorageCard ScanDrop Bio The file extension for CHIPCUVETTE data is cbi Files with previously saved results of measurement can be accessed in the parameter window that will open once the Bio method module is chosen 1 Press Open key in parameter window The standard window for file opening opens 2 Select
12. e DIN ISO 9022 3 2000 e DIN ISO 9022 32 03 0 e DIN ISO 9022 2 2003 01 EG directives The ScanDrop is built and tested to comply with the requirements of EC directives 2006 95 EC and 2004 108 EC It is factory shipped in a faultless and absolutely safe technical condition To preserve this condition and ensure reliable operation the user must follow all safety notes and instructions that are provided in this Manual Integrated accessories or system components from other manufacturers should be treated in accordance with the appropriate user manuals ScanDrop 06 2009 9 Safety Instructions Standards amp Directives 10 06 2009 ScanDrop Technical ScanDrop Data 3 Technical ScanDrop Data Physical amp optical ScanDrop data Technical data ScanDrop Principle Diode array for UV VIS range with additional reference channel Optics Polychromator with concave holographic grid and photo diode line 256 pixels Reference channel polychromator ScanDrop250 or photo diode ScanDrop200 ScanDrop 100 Radiation sources Xenon flash lamp range Wavelength measurement 190 720 nm range Spectral slit width 7 nm Photometric measurement 3 3A Wavelength accuracy 1 nm achieved with holmium oxide filter Wavelength reproducibility Stray light 0 05 nm 0 5 T at 220 nm and 340 nm NaNO Photometric accuracy for VIS 0 02 A at 546 nm with Hellma F4 neutral gl
13. to add a defined wavelength to the list Selected wavelengths are shown in the list field e To delete a wavelength from the list field mark this wavelength and press Del Two wavelengthsRecords the readings for two wavelengths and determines with difference the difference amount between them On selection of this option the two inputs fields Wavelength 1 and Wavelength 2 come on display e Use superimposed keypad to specify a wavelength at each field Selects single measurement or cyclic multiple measurement The results of cyclic multiple measurement for example a series of spectrums are saved in a single cyclic file Option No Description Performs single measurement cycles normal measuring mode 92 06 2009 ScanDrop User Defined Measurement UV VIS Module Settings for amp Management of Measurement Parameters Automatic Time controlled Manual Start request Sets automatic repetition of a sample measurement Define a desired number of repeat cycles at the Number input field Sets time controlled repetition of measurement for kinetic purposes On activation of this button a table will show It allows you to specify up to four different subphases intervals with different interval settings Waiting time The amount of delay form a starting command to the actual start of measurement This time length can be used as incubation time for kinetic jobs Time interval s De
14. 2 Select path and file name and press OK for saving Data can be exported to a text file where they will be available for further pro cessing in other programs for example Excel Data exports can be accom plished via the results window 1 Press Export key in results window The standard window for file saving opens 2 Select path and file name and press OK for saving 9 1 4 Presettings in Quantification Module Presettings can be made for analytical parameters These will be shown on triggering of the Quant module 1 Press Default key in Quant module window 2 Make parameter settings as follows Sample type Type which is preset in the sample table Wavelength Analytical wavelength Multiple meas Specifies number of repeat measurements for a given sample Concentration is determined from the mean value of all absorbance readings Unit Unit of measure for concentration analysis Sample number Number of samples in sample table Display decimals Number of decimal digits in analytical result output 80 06 2009 ScanDrop Quantitative Analysis Quantification Module Presettings in Quantification Module Calibration Selects type of concentration analysis allows entry of factor or name of calibration file where appropriate 3 Use OK to exit the Quant module Default Settings window Use another OK to confirm you want your changes saved All modified presettings will be saved and displayed with
15. Consequently analytical parameters can equally be loaded from a parameter file or a result file 1 Press Open key in results window 2 Select par file and press OK for acknowledgement Analytical parameters will be displayed in the parameter window They can be edited or used for subsequent measurement ScanDrop 06 2009 69 User Defined Measurement UV VIS Module Display amp Management of Measured Results 8 5 2 Performing Sample Measurement 1 Prepare analytical parameters 2 Load CHIPCUVETTE in accordance with sample setup specified via analytical parameters and insert CHIPCUVETTE into holder frame 3 Press Start to trigger measurement Measurement starts with predefined settings and included samples are automatically evaluated in accordance with the sample setup you have configured 8 5 3 Display amp Management of Measured Results Presentation of measured results On completion of measurement the results are displayed on the Chart the Table and the Settings tab of the UV VIS module window The function keys on this window are intended for saving or exporting measured results or restoring the parameter window screen Chart tab Only for measurement of spectrums and kinetic reaction processes A measured spectrum can be graphically shown on the Chart tab The list fields above the spectrum graph area allow you to select a sample and sample position for display Chart Table
16. and brief lateral free space between the instructions on top of ScanDrop system and the cardboard wall 9 Close packing case and use adhesive tape for sealing 100 06 2009 ScanDrop Disposal 12 Disposal It is the ScanDrop owner operator s responsibility to dispose of waste matter sample material resulting from measurement in accordance with statutory rules and local practices Under currently bindung provisions of law the system with electronic components must be disposed as electronic waste on expiry of its specified lifetime ScanDrop 06 2009 101 Disposal 102 06 2009 ScanDrop List of Illustrations 13 List of Illustrations Fig 1 General view Of ScanDrop c cccececeeeeeeceeeeeeeeeeeeeeeseeeesaeeseneees 16 Fig 2 Connector terminals at HID Pro 320 control Unit eee 17 Fig 3 Connector terminals on the rear side of the ScanDrop 18 Fig 4 Structure of a CHIPCUVETTE ccccsescccssssessesssssssessstesteetseesesen 21 Fig 5 Holder frame with CHIPCUVETTE inserted ccccssssesceseeseeees 23 Fig 6 Standard tube installed in measuring chimney ceeeeeee 24 Fig 7 Main menu of ASpect Nano Software n 25 Fig 8 Settings window General tab ccccceceeeeeeeeeeeeeeeseeeeeseeeeeeeees 28 Fig 9 QUANT module window sample assignment settings for GHIPGUVET TE neoe ai EEEE Ea 33 Fig 10 Quant module Chip for defin
17. and managed working in UV VIS module window 1 Click Para to open the UV VIS module Parameters measurement parameter window in UVVIS module The measurement parameter window opens This window contains five tabs General Meas mode Cycles 1 8 9 16 They allow settings for measurement parameters The function keys are located ScanDrop 06 2009 65 User Defined Measurement UV VIS Module Setting amp Managing Measurement Parameters in the window s bottom bar They are intended for management of measurement parameters 8 5 1 1 Measurement Parameter Settings The measurement parameter window provides the following tools for user selections General tab Title For assigning a name to a measurement The specified title will be saved together with the measured results and displayed when this result file is loaded again A name entry at the Title input field is optional Operator This entry is defined by the user identity management tool Display Defines magnitude for ordinate of measured results display during measurement e Absorbance e Transmittance e Energy Accumulations Defines the number of repetitions of a single measurement The readings obtained for a total number of single measurement cycles will be averaged for a selected spectral range and output as the result Accumulation of single measurement readings helps improve the signal to noise ratio Meas mode tab The Meas mode tab provides f
18. automatically analyzed following your preselected sample setup 8 3 3 Display amp Management of Analytical Results Representation of measured results On completion of measurement the results will be represented on the Results and the Settings tab of the results window Its function keys can be used to save or export measured results or to return to the parameter window Tabs in results window Results tab The Results tab contains a sample table with a summary of analytical results ScanDrop 06 2009 51 Bio Method Module Display amp Management of Analytical Results Results Settings Probe 1 e Probe 2 2 Probe 3 a Probe 4 s Probe 5 Probe 6 Probe 7 Export Module Bio result list Pos Sample name methode frest Corr 320mm B Referenz Abs 280 F 1 38 1 7117 Abs 280 F 1 38 17242 Abs 280 F 1 38 1 4862 Abs 280 0 9027 Abs 280 0 7532 Abs 280 0 5933 Fig 22 Bio method Chip display of results list Values on display Pos Sample name Method Result Check box Corr 320 nm Position number of sample on CHIPCUVETTE Sample name defined via sample properties prior to measurement Analytical method Analytical result Performs background correction if active The absorbance value measured at the wavelength of 320 nm is subtracted from the measured absorbances By pressing a sample line the Single sample result window with more sample
19. can be selected from the Calibration list field Factor Performs concentration analysis based on factor Enter desired value at Factor input box which opens Current sample Performs concentration analysis following a calibration graph table that is recorded in a current analytical process as defined via the sample table Select one of three regression calculation possibilities in Regression input field 74 06 2009 ScanDrop Quantitative Analysis Quantification Module Analytical Parameter Settings Y B X Y A B X Y A B X C X Load calibration Loads a previously saved calibration graph refer to section file below Loading previously saved calibration graph From a previously saved result file you may load the calibration data for use in a current measurement job The default folder setting for file saving is StorageCard Scandrop Quant The file extension is squ 1 Set Load calibration file option in Calibration list field 2 Click the I symbol in standard dialog field for opening files 3 Mark a desired file and confirm your selection with OK Calibration data are loaded from the previous file and will be available for use in subsequent concentration analysis Note No other standards can be measured or considered for calibration when you work with a previously saved calibration graph Defining sample sequence and sample properties A sample sequence and sample properties can be specified
20. firmware and software now query The Scandrop Update Tool window opens 6 Press Update Software key or Update Firmware as appropriate for a required update The progress of updating will be displayed The process takes 2 to 3 s 7 Press Close key on completion of an update process Following a firmware update the ScanDrop must be restarted i e power must be turned off and on again Software updates will be valid at once without a restart 98 06 2009 ScanDrop Packaging for Transportation 11 Packaging for Transportation The ScanDrop may only be transported or dispatched for shipment in its original packing A Caution Transport damage You are advised to strictly follow all packaging instructions This is necessary to prevent transport damage 1 Prepare ScanDrop with 2 Pack ScanDrop and HID Pro HID Pro 320 operation control 320 into plastic bags to protect unit for transportation from moisture and scratching n Ea a 3 Place ScanDrop with HID Pro 4 Place second shape moulded 320 s into lateral shape moulded padding part to the system from padding part the top ScanDrop 06 2009 99 Packaging for Transportation 5 Place ScanDrop into its packing 6 Put accessories cardboard box case Make sure it is correctly with CHIPCUVETTES power seated pack and power cable into separate packing unit 7 Insert accessories box into the 8 Place manual
21. from the various analytical modules for triggering Configuration settings for the quick start menu can be made on the Quick start tab of the ScanDrop Settings window 1 Press Settings key in main menu and change to Quick start tab in ScanDrop Settings window 2 Select desired quick start position from Select button list field for editing Quick start button 1 6 Set tube type in Measurement list field Select analytical module in Start module list field Load method Press key I to open the standard window for file opening Select method and press OK for acknowledgement Note On loading only such methods will be displayed which were previously created for the selected tube type 6 Enter a description in field Description of quick start menu If quick start is the active version for starting menu a method list will appear as soon as the ScanDrop is powered up A method can be loaded and displayed by pressing the pertaining Start key 7 1 4 General System Settings The Device tab in ScanDrop Settings window contains basic settings for determination and processing of measured values It displays the current software version and facilitates automatic firmware software updates via USB stick gt section Firmware amp Software Updating p 98 Note Basic settings solely intended to be modified by service personnel Basic settings have been made in such a way that optimal measuring results will be obta
22. in shallow channel Typ e 1 mm Defines sample type in deep channel Method Select other bio method if necessary Alternatively required sample names can be imported from a file in csv format This CSV file must contain the sample names in question in the same order as the sample table Note that the positions of reference samples must be taken into account ScanDrop 06 2009 49 Bio Method Module Settings for amp Management of Analytical Parameters Defining general analytical parameter settings Module Bio x 1 8 9 16 Sample Prop Settings Title 196 rabbit Unit mami Accumulations 10 New Load Save Open Start Eject Default Close Fig 21 Bio module Chip general analytical parameter settings The wavelength is automatically set for a selected method The following parameter settings options on the Settings tab are valid to all methods Title Title of a measurement This entry is optional Unit Unit of measure for analysis The unit of measure appears in the results window It is saved together with the analytical results This entry is optional Accumulation Defines number of repeat measurement runs for a single reading From the total number of single readings a mean value for the selected wavelength is obtained and output as the result Accumulation of single readings helps improve the signal to noise ratio Saving and loading analytical parameters Analytical parameter
23. layer thickness Abs 0 1mm Absorbance value for 0 1 mm layer thickness In the event of non compliance with these requirements affected test reading s will be marked as faulty with a supporting reference to non conforming sample preparation To facilitate this analytical procedure also when performing concentration analysis with calibration one calibration graph is recorded for the deep and one for the shallow test point to be included for analysis Triggering Quantification module 1 For ScanDrop 250 Select CHIPCUVETTE tube type in main window 2 Press Quantification key ScanDrop 06 2009 35 Quantitative Analysis Quantification Module Parameter Settings for Concentration Analysis The Quant module parameter window opens Function elements in Quant module parameter window The Quant module parameter window has four tabs 1 8 and 9 16 Sample prop Settings Tube setup with CHIPCUVETTE sample assignments Means sample table with details regarding sample sequence sample type name and standard concentrations Defines measurement and evaluation settings for concentration analysis The actual function keys are located at the bottom bar of the dialog window Function New Load Save Open Start Eject Default Close Description Deletes all previous entries and allows reentry of analytical parameters Loads previously saved measuring and evaluation paramete
24. method can be selected via tabs 1 8 and 9 16 in the sample setup area 1 Define reference sample 2 Activate Meth key 3 Select analytical method in list field 48 06 2009 ScanDrop Bio Method Module Settings for amp Management of Analytical Parameters 4 Mark all sample positions you want analyzed according to the selected method Selected samples will be marked in the color of the selected method to the right of the list field Different sample channels of a CHIPCUVETTE can be analyzed according to different methods in one measurement process However for a given sample channel the same method must be defined for both test points or one test point may be deactivated Entering sample sequence and methods in a table The Sample table Tab displays a table with sample names and analytical methods Module Bio 1 8 9 16 Sample Prop settings Load sample names from file csv Reference Sample 1 Absorbance 280 Factor 1 38 3 Sample 2 Absorbance 280 Factor 1 38 a Sample 3 Absorbance 280 Factor 1 38 s Sample 4 Absorbance 280 e Sample 5 Absorbance 280 Fig 20 Bio module Chip for configuration or modification of sample table 1 To enter or change sample properties press the particular sample line A window for definition of properties for a selected sample opens 2 Available value entries Sample name Defines sample name Type 0 1mm Defines sample type
25. nm to 720 nm Its integrated portable operation control unit with HID Pro 320 color touchscreen makes the ScanDrop a footprint saving stand alone tool The operation control unit has implemented ASpect Nano software to facilitate e Quantitative evaluation based on three calibration models e Biochemical analysis e Analysis following user programmed formulas e Intensity measurement at discrete wavelengths and within discrete spectral ranges plus output and display of readings in units of transmittance absorbance and energy Optionally the ScanDrop may be connected to a PC Operation control and data evaluation processes are accomplished by WinASPECT software for UVVIS spectroscopy in this case ScanDrop 06 2009 5 General Advice 1 2 Notes Regarding the Use of This Manual A A Warning and information symbols used throughout this Manual Danger Advice of this kind must be followed under all circumstances in order to prevent physical harm to people Caution Advice of this kind must be followed in order to prevent damage to the product Note Advisory notes should be followed in order to obtain correct results of measurement For greater convenience in working with this Manual the following systematic rules have been incorporated herein e Chapters and illustrations are numbered consucutively e Each picture has its own caption e Cross references to other sections are marked with an arrow e g gt secti
26. on the Sample table tab Each sample is assigned a sample name a standard concentration weighed in portion and dilution factor in a sample table Module QUANT x General SampleTable Number 10 Load sample names kaad aa 2 stai so 6 5 Abs 3 std 22 std3 so 60 6 All ia Eme x Fig 37 Quant module Stand definition of sample table 1 Define total number of samples including reference and standard sample in the Number field using arrow keys as necessary The sample table is built up Through modification of the total sample number you may extend or reduce the sample table as required A change is always made at the end of the sample table ScanDrop 06 2009 75 Quantitative Analysis Quantification Module Analytical Parameter Settings 2 Assign a type to the samples in the table Activate a required type switch on the window s left hand side then tap on that cell in the Type column of the table you want assigned to the selected type The following sample types can be defined in Quant module Ref Reference Note Reference measurement must precede measurement of any other sample For this reason this type of measurement must be defined as the first in a sample table Std Standard with known analyte concentration Conc Sample for which a concentration is to be determined Abs Sample for which only absorbance is measured Assign sample name Tap onto the Sample name inp
27. settings can be handled with the help of the two keys Load and Save in the parameter window The default path for file saving in Bio method module is StorageCard ScanDrop Bio The file extension is cbi Saving a file of analytical parameter settings 1 Select analytical parameters you want saved in parameter window 2 Press Save key The standard window for file saving opens 3 Set path and file name and press OK for acknowledgement 50 06 2009 ScanDrop Bio Method Module Display amp Management of Analytical Results Loading of analytical parameters Analytical parameter settings are saved in the same manner as measured results A file always contains a set of analytical parameters A result file contains the absorbance readings for the measured samples in addition Accordingly analytical parameter settings can equally be loaded from a parameter file or a result file 1 Press Load key in parameter window 2 Select cbi file and press OK for acknowledgement Analytical parameter settings are displayed in the parameter window They can be edited or used for subsequent measurement 8 3 2 Triggering Measurement 1 Prepare analytical parameter settings 2 Load CHIPCUVETTE in accordance with sample setup defined in analytical parameters and insert CHIPCUVETTE into holder frame 3 Click Start to trigger measurement Measurement begins with predefined parameter settings The samples are
28. standards are always measured in both channels Target value Known analyte concentration of standard samples Dilution Dilution factor Init weight Concentration factor for correction of a weighed in initial portion Alternatively sample names can be imported from a file in csv format This CSV file must contain sample names in an order identical with that of the sample table The positions of reference and standard samples must be taken into consideration Defining measurement parameters For measurement parameter settings and definition of a desired type and mode of concentration analysis the Settings tab is provided Module QUANT x 1 8 9 16 Sample Prop Settings Title rabbit IG Wavelength 280 Multi measure 1 Unit majml Accumulations 5 Calibration Current sample table Regression ly A B X x New Load Save Open Start Eject Default Close Fig 12 QUANT module Chip measurement parameter settings Title Title of a measurement This entry is optional Wavelength Designates the wavelength being analyzed Multiple meas The number of repeat measurements of a sample Concentration is determined from the mean value of all readings 38 06 2009 ScanDrop Quantitative Analysis Quantification Module Parameter Settings for Concentration Analysis Unit Unit of measure for concentration analysis This input appears as part of results display It is saved together with th
29. the bottom bar of the dialog window Function Description Para Opens parameter window for measurement parameter settings Open Opens a previously saved result file Save Saves the results of current measurement Export Exports measured results Ref Triggers reference measurement Start Triggers measurement with selected measurement parameter 90 06 2009 ScanDrop User Defined Measurement UV VIS Module Settings for amp Management of Measurement Parameters 9 4 1 settings Close Closes UV VIS module Settings for amp Management of Measurement Parameters Options for measurement parameter settings and management are provided in the UV VIS module Parameters window 1 Press Para key in UV VIS module window The UV VIS module Parameters measurement parameter window opens This window comprises three tabs General Meas mode Cycles that allow settings for measurement parameters Related function keys at the bottom bar of the window are intended for management of measurement parameters Module UY IS Parameter x General Meas mode Cycls Title Spectrum Operator Display Accumulations 10 New Open Save Ok Cancel 9 4 1 1 Measurement Parameter Settings The following setting options are provided in measurement parameters window General tab Title For definition of a title to a measurement task The entry for title will be saved together with the ac
30. 6 2009 ScanDrop Bio Method Module Presettings in Quant Module If these requirements are not fulfilled the given measured value will be marked as faulty with a supporting reference note to non conforming sample preparation Triggering Bio method module 1 For ScanDrop 250 Select CHIPCUVETTE tube type in main window 2 Press Bio method key The Bio module parameter window opens Module Bio x 1 8 Jo 16 Sample Prop Settings Meth DNA Purity 260 280 Ref 1 2 3 4 5 6 7 8 a all New Load Save Open Start Eject Default Close Fig 18 Bio module Chip parameter window Function elements in Bio module parameter window The Bio module parameter window provides four tabs 1 8 and 9 16 Tube setup with CHIPCUVETTE sample assignments Sample prop Samples table with details regarding sample sequence sample type name and standard concentrations Settings Allows input of measuring and evaluation parameters for Bio method analysis All function keys are located at the bottom bar of the dialog screen Function Description New Deletes all previous entries for definition of new analytical settings Load Loads previously saved analytical settings for subsequent analysis Save Saves current analytical parameter settings Open Opens previously saved file of analysis performed with Bio method Start Triggers measurement with currently set an
31. Bio Mehod Module 84 Presettings in Bio Method Module cccseeeeeeeeeeeeeeeeteeeeeeeeeeees 85 Expression MOJU ic cusshckertetobvecsetevbiceedheetepeith este devbest AREE RN TAKEREI 86 Triggering Measurement amp Displaying Analytical Results 87 Data Managemen t ccceecceeeesecceeeeeeeeeeeeeeeneeeeeeeneeeesneneeseenennaeeeenenees 88 Analytical Parameter Presettings Programming of Analytical FOrmula ccccccceceeeceesceeeeeeeeeeeeeecaeeeeeeeseeeeeseaeeeeeaeeeeeeeee 88 User Defined Measurement UV VIS Module cceeseeeereees 90 Settings for amp Management of Measurement Parameters 91 Performing Sample Measurement cccccsceceeeereeceeeeeeeaeeeeeeeeeeees 94 Display amp Management of Measured Results ccccceeeeseeeereees 94 Preventive Maintenance amp Care esseeseeees 97 Cleaning the ScanDrop cccccceceeeeeseeeeeeeceeeeeaeeeeeeeseeeeeesaeeseeeeeaes 97 06 2009 ScanDrop Content 10 2 Cleaning the Touchscreen cccceeeeeceeeeeteeeeeeeseeceeeeesaeeesaeeneneeeeaees 98 10 3 Firmware amp Software Updating ccccccceeeeeseeeeeeeeeeeeeeeseaeeeeeeeeenees 98 11 Packaging for Transportation ssssssssssssnnnnnnnnnnnnnnnnn 99 12 Disposal dasima aeae eae eeann esarete 101 13 List of Illustrations ccceceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeees 103 ScanDrop 06 2009 3
32. acilities for selection of a spectral range or of the wavelengths to be measured Spectrum Measures spectral range Set starting wavelength and end wavelength of a spectral range at the two input fields Wavelength 1 and Wavelength 2 Fixed Measures at one or more wavelengths wavelengths On selection of this option the Wavelength input field will appear with a great list field e Having pressed the Wavelength input field use the numerical keypad which is superimposed on the display area for entering a desired wavelength e Use 4 to hide the keypad again when you have completed the last wavelength entry e Press Add to acknowledge a wavelength 66 06 2009 ScanDrop User Defined Measurement UV VIS Module Setting amp Managing Measurement Parameters The selected wavelengths will appear in the list field e To delete a wavelength from the list field mark this wavelength and press Del Two wavelengthsRecords measurement readings for two wavelengths and and difference determines their difference amount e On selection of this option the two input fields Wavelength 1 and Wavelegth 2 will show Use the numerical keypad which is superimposed on the display area to define a wavelength at each input field Module UY YIS Parameter x General Meas mode cyds 1 8 9 16 Meas mode Wavelength i00 New Open Save Ok Cancel Fig 31 UVVIS module Chip parameter settings for wavelengths
33. alytical parameters Eject Moves holder frame of CHIPCUVETTE out for insertion or removal of a tube of this type ScanDrop 06 2009 47 Bio Method Module Settings for amp Management of Analytical Parameters Default For definition of presettings These are displayed in Bio module before a new analytical process starts Close Closes Bio module session 8 3 1 Settings for amp Management of Analytical Parameters Automatically on selection of a bio method the appropriate measurement parameter settings are loaded Consequently only a bio method needs to be selected and a sample assignments chart be defined for working with a CHIPCUVETTE Entering sample sequence Action for sample assignment settings of CHIPCUVETTE is described in section Defining Sample Assignments for CHIPCUVETTE in ASpect Nano p 33 For measurement in Bio module tabs 1 8 und 9 16 provide the following sample options Meth Sample which is analyzed according to the selected method Ref Reference Note Reference measurement must always precede sample measurement No Deletes single sample channels All Assigns all sample channels to the sample type that was selected above Module Bio 1 8 Jo 16 Sample Prop Settings Meth absorbance 280 Factor 1 38 Mi Ref mec E No Load Save t Default Close Fig 19 Bio module Chip sample sequence settings An analytical
34. alytical module with its pre settings or a quick start menu will show immediately after power is turned on Triggering analytical module after ScanDrop is turned on Initial screen settings can be made by selecting the Launch ASpect Nano Software group on the General tab of the ScanDrop Settings window ScanDrop Settings General Device Quick Start CHIPCUVETTE logged User Language JEnglish Launch AspectNANO software CHIPCUVETTE bd Measurement Start module Fig 8 Settings window General tab 1 Press Settings key in main menu and change to General tab in ScanDrop Settings window Select tube type for analysis in Measurement list field Select starting software in Start module list field as shown hereafter Mail Basic setting with window for selection of an analytical module Quick start Menu with up to six analytical methods available for 28 06 2009 ScanDrop Operation Control with ASpect Nano General Settings for ASpect Nano measurement Quant Module for concentration analysis Bio method Module with prefabricated typically required methods of bio analysis Expression Module for analysis based on user programmed formula s UV VIS Module for measurement of absorbance transmittance and energy at discrete wavelengths or for discrete spectrums Configuring quick start menu The quick start menu is provided to load methods or measurement parameters directly
35. alytical wavelength Unit Unit of measure of concentration value Multiple meas The number of repeat measurements that is defined for determination of a concentration value Calibration Calibration model Coefficients calculated according to calibration model Standard deviation with linear and square calibration models Valid calibration range Module QUANT Result list Calibration Table Settings Title Operator Wavelength 500 Unit Multi measure 1 Calibration Current sample table Y A BX R2 0 9998 Range of concentration 0 0073 Min 6 5 B 0 0099 Max 125 C 0 Save Export New Close Fig 40 QUANT module Std view of parameter settings for current analytical job ScanDrop 06 2009 79 Quantitative Analysis Quantification Module Presettings in Quantification Module Opening saving and exporting measured results Files with previously saved results of measurement are displayed in a parameter window that will appear once the Quantification module has started The default path for file saving in Quantification module is StorageCard ScanDrop Quant The file extension is squ 1 Press Open key in parameter window The standard window for file opening opens 2 Select squ file with quantitative results of measurement and press OK for loading Measured results can be saved in the results window 1 Press Save key in result window The standard window for file saving opens
36. analytikjena ScanDrop Nano volume Spectrophotometer 4 PEE Analytik Jena AG analytikjena Kundendienst Konrad Zuse Str 1 07745 Jena Phone Hotline 49 3641 77 7407 Fax 49 3641 77 7449 e mail service analytik jena de General information about Analytik Jena AG on the internet http www analytik jena de Copyrights and Trademarks Microsoft Windows 2000 Windows XP Windows NT MS Excel are registered trademarks of Microsoft Corp Publication No 211 802 23 Edition June 2009 Technical documentation made by Analytik Jena AG This documentation describes the state of this product at the time of publishing It need not necessarily agree with future versions of the product Subject to change Copyright 2009 Analytik Jena AG Content Table of Contents 1 General AQVICE isccssccreccieisniacteueue civeravnaateetesdiclavnaiverateadaal 5 1 1 Working with the SCanD oOp ccccecesceceeeeeceeeeeeneeeseeeeeseaeeesaeeeeneeeeaees 5 1 2 Notes Regarding the Use of This Manual cc cceeeeeeeesteeeeeeeeeees 6 2 Safety INSHrUCHONS sesdiisciisiniacdcedieainenveansnnndestieianeaneanstenecs 7 2 1 General Safety INStruCtiONS cccceceeeeeeeeeee tees eeeeeeseeeeetaeeeeeaeeteneeess 7 2 2 Standards amp DireCtives ccecccccseeeseeeeeeeceeeeeeeaaeeeeneeseeeesaeeseaaeesenees 9 3 Technical ScanDrop Data cccccesseseseseeeeeeeeeeeeeeees 11 4 Transportation amp Installa
37. and samples for concentration or absorbance measurement will be processed Working with the Quantification module it is possible to prepare and save required methods to such an extent that a given analytical process can be triggered by pressing Start once a method and sample assignments setup have been selected A sequence of samples for analysis with a CHIPCUVETTE is defined through that tube s sample assignments The Quantification module displays an initial parameter window for input of required analytical settings when selected It outputs the results for concentration in a separate results window in graphical and numerical form Automatic determination of optimal measuring range Where quantitative analysis performed with a CHIPCUVETTE records a test reading at both test points of a sample channel the analytical process will use that of the two test readings which is found to be within the linear measuring range from 0 005 to 1 5 Abs If both measured readings are found to be within this range the one which is closer to 0 75 Abs will be included for analysis This automated procedure for determination of an optimal measuring range allows you to extend the available width in concentration without having to put up with a dilution factor of 10 The measured readings are tested for accuracy according to these criteria e Abs imm gt Abs 0 1 mm e Abs 1mm gt 0 005 e Abs 0 1 mm lt 1 5 Abs 1 mm Absorbance value for 1 mm
38. ass filter Photometric accuracy for UV 0 04 A with potassium dichromate after Ph Eur Longtime stability 0 003 A h at 500 nm Baseline noise 0 002 RMS Shortest time to measure 12 ms for one spectrum taking into consideration tolerances prescribed by the selected standard ScanDrop 06 2009 Technical ScanDrop Data General technical ScanDrop data Footprint area W x D about 500 mm x 450 mm Phys dimensions Wx Hx 286 mm x 235 mm x 250 mm D Input voltage 24V DC 2A External power pack Inlet 100 240 V AC 50 60 Hz Output voltage 24V DC 2 7A Radio interference EN 55011 class A suppression Degree of protection IP 20 Data terminals USB Summary table 3 1 General technical ScanDrop data Technical data of HID Pro 320 Phys dimensions WxHx 110 mm x110 mm x 180 mm D Display Touch screen 5 7 diagonal Operating system Windows CE 12 06 2009 ScanDrop Transportation amp Installation Requirements 4 Transportation amp Installation Requirements 4 1 Transportation amp Storage For transportation and storage the following ambient conditions must be maintained Temperature range 40 C 50 C Rel air humidity up to 95 Use dessicant 4 2 Installation Requirements A Caution The following compulsory rules must be observed at any time during operation Do not place objects onto the ScanDrop Prevent operat
39. at opens on selection of the Quantification module The default path for file saving in Quantification module is StorageCard ScanDrop Quant The file extension is cqu 1 Press Open key in parameter window The standard window for file opening opens 2 Select cqu file with quantitative measuring results and press OK for acknowledgement Measured results are saved in the results window 1 Press Save key in results window The standard window for file saving opens 2 Select path and file name and press OK for acknowledgement Files can be exported to a text file where they will be available for further processing in other programs for example Excel Files can be exported when working in the results window 1 Press Export key in results window The standard window for file saving opens 2 Select path and file name and press OK for acknowledgement 44 06 2009 ScanDrop Quantitative Analysis Quantification Module Presettings in Quant Module Closing results window To exit the results window you may select either New or Close The software will query if you want unsaved data to be saved and restore the Quant module parameter window New The parameter window will show basic settings or presettings Close The parameter window preserves the parameter settings of a previous measurement These can be used or modified for next measurement 8 2 4 Presettings in Quant Module Presett
40. dividual assemblies or single parts This will also apply in the event of service or repair work which is not performed by authorized service personnel All claims for warranty guarantee will be null and void in such cases Local requirements practice Local safety provisions and rules which are applicable to product operation should be followed e g industrial labor protection practice accident prevention rules industrial safety regulation A reference to potential hazards contained in the User Manual must not be regarded as replacing compulsory workforce safety requirements Personnel The ScanDrop may not be operated by anyone other than duly trained and qualified personnel Prior knowledge of this Manual is indispensable and assumed to have been acquired before operation Shut down in the event of emergency In the event of an emergency the power plug must be detached from the line socket in order to cut power supply to the ScanDrop and its components Caution There is danger of loss of data and damage to the operating system Refrain from operation in rooms with an explosive atmosphere ScanDrop 06 2009 7 Safety Instructions General Safety Instructions A A Danger of finger injury working at the ScanDrop 250 Keep your fingers clear of the standard tube chimney As part of outward motion the CHIPCUVETTE cover is raised to a position above the tube chimney which creates danger of physical injury
41. dministrator Password admin User identity management The user identity management tool can be accessed via the Gneral tab of the ScanDrop Settings window Creating a new user profile 1 Press Settings key in main menu and change to the General tab in the ScanDrop Settings window 2 Press Edit user list key to open a summary table of available user profiles Press Add key to open the dialog window for entry of a new user profile Make data inputs as follows o User name 26 06 2009 ScanDrop Operation Control with ASpect Nano General Settings for ASpect Nano Password and repeated password User level refer to section User levels amp rights below Deactivate User is blocked check box If this check box is active a currently edited profile will be blocked so it cannot be used 5 Press OK to exit the dialog window The new user profile is now contained in the list of profiles Editing a user profile 1 Select desired profile from the summary list of available user profiles 2 Press Edit key to open the dialog window with user profile data 3 Working in the dialog window you may edit the password user level and the blocked state 4 Press OK to acknowledge your changes in data settings Note A user profile can be edited or blocked but it cannot be deleted User levels Working in the user identity management you may assign rights on three levels Administrator Extended us
42. e 1 Select CHIPCUVETTE option from the Select measurement list and select a desired application under Modules working in the main menu 2 Press CHIP key to move the CHIPCUVETTE out of the ScanDrop Place CHIPCUVETTE onto the holder frame Then slide the CHIPCUVETTE into the left holder groove and press it down Note Make sure that the CHIPCUVETTE is correctly positioned Its fill openings must be facing to the top and position 1 must be located on the left hand side A check for correct placement can be made using system setting procedures section System Settings for CHIPCUVETTE p 30 The CHIPCUVETTE will mechanically engage with a perceivable click 4 Acknowledge Please insert CHIPCUEVETTE screen prompt with OK The CHIPCUVETTE holder moves into the ScanDrop 5 Press CHIP again on completion of measurement in order to trigger another outward motion run of the holder frame with CHIPCUVETTE Place your fingertip into the right hand recess in the frame below the CHIPCUVETTE push the tube somewhat to the left and take it off from the top 22 06 2009 ScanDrop Inserting Samples Working with a Standard Tube 6 5 4 1 Holder frame of CHIPCUVETTE 4 Recess in holder frame 2 Shallow test point of sample channel 1 5 Clamp 3 CHIPCUVETTE 6 Groove Fig 5 Holder frame with CHIPCUVETTE inserted 6 2 Working with a Standard Tube Only for ScanDrop 250 and ScanDrop 100 Cr Note
43. e parameter window They can be edited or used for subsequent measurement 9 4 2 Performing Sample Measurement 1 Prepare analytical parameters 2 Place reference sample into tube chimney 3 Trigger reference measurement by pressing Ref 4 Place tube with sample being analyzed into tube chimney 5 Press Start key to begin measurement Measurement starts with predefined measurement parameters The various samples are automatically evaluated in accordance with your sample assignment presettings 9 4 3 Display amp Management of Measured Results Presentation of measured results On completion of measurement the results can be viewed on the Chart the Table and the Settings tab of the UV VIS module window Its function keys are intended for saving or exportation of measured results and for returning to the parameter window Chart tab Only for measurement of spectrums and kinetic reaction graphs A measured spectrum is graphically shown on the Chart tab Module UYYIS Chart Table Settings Fig 47 UVVIS module Std display of spectrum Table tab Table contains a sample table with a survey of the measured results 94 06 2009 ScanDrop User Defined Measurement UV VIS Module Display amp Management of Measured Results Settings tab The following measurement parameter data can be viewed and saved together with the measured results Meas mode Spectral range or wavelength being m
44. e result of analysis Accumulation Designates the number of repetitions of measurement you want included in the formation of a measured value All single readings are averaged and output as the result of measurement Absorbance Accumulation of single measured readings may help improve the signal to noise ratio The type of concentration analysis can be selected in the Calibration list field Factor Concentration analysis using a factor Enter required value having opened the Factor input field Note In the case of concentration measurement using a factor measured absorbance readings will be normalized to a layer thickness of 1 cm for both test points of a sample channel For this reason the extinction coefficient for 1 cm layer thickness must also be defined Current sample Concentration analysis relies on a calibration graph that is table recorded as part of a current analytical job in accordance with the sample table entries Select one of three regression calculation options in the Regression input field Y B X Y A B X Y A B X C X Load calibration Loads a previously saved calibration graph refer to section file further below Loading previously saved calibration graph From a previously saved result file the settings for calibration can be loaded for use in a current measurement task 1 Select Load calibration file option in Calibration list field 2 Click symbol to open the standard dialog screen for file openi
45. eaeeseeeeeeeeeessaeeesaeeseaes 29 7 1 5 System Settings for CHIPCUVETTE 0 eeccceeseseeeeeeeeeeeeeseeeeeeaeeeeees 30 7 2 Data Management on HID Pro 320 0 0 eee eeeeeeee center eeenaeeeeeeaeeeeeeaaaes 32 8 Measurement in CHIPCUVETTE sssseeeeeeesseeseeeenes 33 8 1 Defining Sample Assignments for CHIPCUVETTE in ASpect Nano 33 8 2 Quantitative Analysis Quantification MOdUIe cccceesseeeeeees 35 8 2 1 Parameter Settings for Concentration Analysis ceseeeeeees 36 8 2 2 Triggering Concentration Measurement ccceeeeseeeeeteeeeeeeeeneeeees 40 8 2 3 Display amp Management of Measured ReSUIts cc ccceceessteeeeeereees 40 ScanDrop 06 2009 1 Content 8 2 4 8 3 8 3 1 8 3 2 8 3 3 8 3 4 8 4 8 4 1 8 4 2 8 4 3 8 4 4 8 5 8 5 1 8 5 2 8 5 3 9 1 9 1 1 9 1 2 9 1 3 9 1 4 9 2 9 2 1 9 2 2 9 2 3 9 3 9 3 1 9 3 2 9 3 3 9 4 9 4 1 9 4 2 9 4 3 10 10 1 Presettings in Quant MOUIC cccceeeeseeeeeeeeeeceeeeeeeteeeeetaeeeeeeeees 45 Bio Method Module tcc e eaa rai enian a aaaea aaa a a niaaa aeei 46 Settings for amp Management of Analytical Parameters 0 00 48 Triggering Measurement sssssesssriessernsssrrestsnnesnnnaestennessnnnnnnnnnnnennna 51 Display amp Management of Analytical Results c cccceeeeseeeereees 51 Presettings in Bio Module asssesssssesssrnssssrresssnnesrnnnnntennennnnnnnnnnnennnne 54 Expres
46. easured Display Displays unit of measure for ordinate of measured results during measurement Accumulations The number of repetitions of a single measurement cycle Interval Settings for cyclic measurement Opening saving and exporting measured results Files with previously saved results of measurement can be opened in the UV VIS module window The default path for file saving in UV VIS module is StorageCard ScanDrop UVVIS The file extension is das 1 Press Open key in UV VIS module window The standard window for file opening opens 2 Select desired das file with analytical results from UV VIS module and press OK to open this file Measured results can be saved in the UV VIS module window 1 Press Save key in result window The standard window for file saving opens 2 Select path and file name and press OK to save this file Data can be exported to a text file where they will be available for further processing in other programs for example Excel For exportation of data the UV VIS module window is available 1 Press Export key in result window The standard window for file saving opens 2 Select path and file name and press OK to save the given file ScanDrop 06 2009 95 User Defined Measurement UV VIS Module Display amp Management of Measured Results 96 06 2009 ScanDrop Preventive Maintenance amp Care 10 Preventive Maintenance amp Care The ScanDrop
47. ed with crystal pipettes of a maximum 20 uL capacity The opening on the deeper channel side is intended to be filled using standard pipettes with tips up to 200 uL capacity Optionally or with very small sample quantities a sample channel may only be filled at its shallow or its deep test point end Channel depth Fill quantity Pipette tips 0 1mm 0 3 uL 0 1 20 uL 1 mm 2 uL up to 200 uL both combined max 4 uL 1 2 3 5 4 1 Sample channel 4 Test point for 1 mm layer thickness 2 Fill opening on shallow channel side 5 Fill opening on deep channel side 3 Test point for 0 1 mm layer thickness Fig 4 Structure of a CHIPCUVETTE ScanDrop 06 2009 21 Inserting Samples Working with a CHIPCUVETTE Inserting a CHIPCUVETTE A Caution Danger of finger injury working with the ScanDrop 250 Keep your fingers clear of the tube chimney and take care that no tube or other object are located in the standard tube chimney if measurement is to be performed with a CHIPCUVETTE During outward motion of the CHIPCUVETTE holder the tube s cover raises to a level above the tube chimney s top edge Note Contamination of a CHIPCUVETTE may falsify measured readings Prevent physical contact with optical surfaces test point surface of a CHIPCUVETTE Contamination forming on an optical surface may falsify the readings that will be measured Hold the CHIPCUVETTE only by its lateral sid
48. er Restricted user Load analytical module Start measurement Create analytical method Reregister user Edit general system settings Reregistration of a user During an ASpect Nano session one may change a currently logged user For reregistration the General tab on the ScanDrop Settings window is available 1 Press Settings key in main menu and change to General tab in ScanDrop Settings window 2 Press Log Off key to open the dialog window for user and password entry 3 Enter new user name with pertaining password and press OK for acknowledgement The newly logged user will be displayed at the General tab ScanDrop 06 2009 27 Operation Control with ASpect Nano General Settings for ASpect Nano 7 1 2 Language Settings Language settings can be made on the General tab of the ScanDrop Settings window 1 Press Settings key in the main menu and change to General tab in ScanDrop Settings window 2 Select the Language list field 3 Select desired language German or English The software user screen will promptly convert to the selected language output version 7 1 3 Setting Initial Screen After ScanDrop Power Becomes Available In default setting version the main menu with keys that are assigned to the four analytical modules will be displayed once power is available to the ScanDrop This setting version can be edited so a particular an
49. er in the internal reference channel 7 1 5 System Settings for CHIPCUVETTE Alignment of CHIPCUVETTE Exact positioning of the CHIPCUVETTE within ScanDrop beam train is an indispensable prerequisite for obtaining correct measurement results Alignment is performed under software control using the CHIPCUVETTE tab in the ScanDrop Settings window We recommend that a positional adjustment routine be performed for each new batch of tubes of this type Insert an empty non filled CHIPCUVETTE for this purpose Alignment is carried out using the deep channels Three different modes are available for choice as necessary to meet the requirements on accuracy Single channel calibration fast Monitors channel 3 As measurement is going on the CHIPCUVETTE moves through the beam path thus recording the profile of this sample channel From 30 06 2009 ScanDrop Operation Control with ASpect Nano General Settings for ASpect Nano the recorded profile the location of the mid channel point is then determined and resulting correction factors are calculated Two channel calibration recommended Monitors channels 1 and 16 The alignment process is identical with that in Single channel calibration mode All channel calibration advanced Monitors all channels The alignment process is identical with that in Single channel calibration mode Correction of channel depth A channel depth of 100 um for shallow channel measure
50. es of analytical parameter settings are displayed and saved together with the measured results Title Title of measurement Operator Name of user Wavelength Analytical wavelength Unit Unit of measure of concentration value Multiple meas The number of repeat measurement cycles required to determine a concentration value Calibration Calibration model Samples tab The Sample tab contains a table of samples with an overview of the results of measurement Module QUANT Result list Settings Samples Calibration Coefficients Pos Sample name tye cone Sam le 1 CONC 8 2969 Sample 2 CONC 8 3979 Sample 3 CONC 7 2858 Sample 4 CONC 6 1303 Sample 5 CONC 5 0993 Sample 6 CONC 4 0733 Sample 7 CONC 3 0045 Fig 14 Quant module Chip outputs table with results of analysis The sample table survey area displays the following values ScanDrop 06 2009 41 Quantitative Analysis Quantification Module Display amp Management of Measured Results Pos Position number of sample in CHIPCUVETTE Sample name Sample name as defined in sample properties prior to measurement Type Sample type reference standard concentration value or absorbance value Conz Calculated concentration value On pressing of a sample line a Single sample result window with more individual sample details will open Name Sample name Conzentration Concentration value Target value Only for standards Nominal c
51. ess Save key The standard window for file saving saving opens 3 Set path and file name and press OK for acknowledgement Loading analytical parameters Analytical parameters are saved in the same manner as measured results A file always contains analytical parameters Result files contain the absorbance values of tested samples in addition For this reason analytical parameters can equally be loaded from a parameter file or a result file 1 Press Load key in parameter window 2 Select cei file and press OK for acknowledgement Analytical parameters are shown in the parameter window They can be edited or used for subsequent measurement 8 4 2 Triggering Measurment 1 Prepare analytical parameters 2 Load CHIPCUVETTE in accordance with sample setup defined via analytical parameters and place CHIPCUVETTE into holder frame 3 Press Start to begin measurement Measurement starts with specified measurement parameter settings Samples are automatically evaluated according to sample assignment definitions 8 4 3 Displaying amp Managing Analytical Results Representation of measured results On completion of measurement the results are displayed at the Results and the Settings tab of the results window The window provides function keys that are intended for saving and exporting of measured results as well as for restoring the parameter window ScanDrop 06 2009 59 Expression Module Displaying a
52. ess the corresponding sample line A window for defining properties for a selected sample opens 2 Value entries Name Enter sample name Type 0 1 mm Enter sample type for shallow channel Type 1 mm Set sample type for deep channel Expression Select other formular if necessary Alternatively sample names can be imported from a file in csv format This CSV file must contain the sample names in the same order as in the sample table The position of reference samples must also be taken into account Defining general measurement parameters A wavelength is automatically set for a given selected method The following parameters and entries which were made on the Settings tab will be valid for all methods Title Title of a measurement This entry is optional Accumulation The number of repeat measurements required to calculate an absorbance value cr Note The unit of measure for a measured quantity can be saved in the related analytical formula Saving and loading analytical parameters Analytical parameters can be managed with the help of the two keys Load and Save of the parameter window The default path for file saving in 58 06 2009 ScanDrop Expression Module Displaying amp Managing Analytical Results Expression module is StorageCard ScanDrop EXPRESSION The file extension is cei Saving a file of analytical parameters 1 Set analytical parameters you want saved in the parameter window 2 Pr
53. etted when you work with spray disinfectant Prior to reshipment of a ScanDrop which is used to process infectious material to Analytik Jena AG for necessary service work it must be decontaminated and performed action be documented ScanDrop 06 2009 97 Preventive Maintenance amp Care 10 2 Cleaning the Touchscreen Use typical domestic detergents to clean the touchscreen Do not allow the sensor to come into contact with acetone or solvents This might result in discoloring or haziness of the uppermost plastic layer 10 3 Firmware amp Software Updating Firmware updates can be performed on the Device tab of the ScanDrop Settings window Software update packages will be sent via email Please follow special instructions for unpacking and renaming of files as may be necessary from case to case Save files to a USB stick according to instructions All files which are required for a given update must be contained in the Scandrop Update directory of this USB stick The two text files appfile txt and gsefile txt list all those files which must be contained in the Update folder 1 Turn ScanDrop on and enter your password once the software has started 2 Wait until the main menu shows Then plug your USB stick into the USB stick port on the right hand side of the HID Pro 320 Select Settings menu item in the main menu Press Firmware Software Update on the Device tab Respond Yes to Do you want to update
54. fines the total length of a subphase Time s The time between two successive measurements within a given subphase Multiple sample measurement with manual triggering A single sample measurement is triggered manually After each measurement a dialog window appears You may decide to trigger another measurement or terminate your series of measurements in this window The number of samples in a cycle need not be defined in advance Actual measurement will not start until the Start measurement query has been confirmed if this box is active 9 4 1 2 Saving amp Loading Measurement Parameters Action for management of measurement parameters in UV VIS module can be triggered by pressing Open and Save in the measurement parameter window The default path for file saving in UV VIS module is StorageCard ScanDrop UVVIS The file extension is par Saving a measurement parameter file 1 Select measurement parameters you want saved in parameter window 2 Press Save key The standard window for file saving opens 3 Select path and file name and press OK for acknowledgement Loading analytical parameters 1 Press Open key in measurement parameter window 2 Select desired par file and press OK for acknowledgement ScanDrop 06 2009 93 User Defined Measurement UV VIS Module Display amp Management of Measured Results The file s analytical parameters are displayed in th
55. gger reference measurement 4 Place tube with analytical sample into tube chimney 5 Press Start to launch measurement Measurement starts with predefined parameters Module Expression x Sample name Expression y Abs 4 260 A 280 Accumulations 1 Result Measurement values nm A nm A 1 4502 260 0 8318 280 0 6183 Operator New Open Save Export Ref Start Default Close Fig 44 Expression module Std display of results The results of analysis can be viewed in the lower half area of the Expression module window In addition to the calculated result it outputs the values that have been obtained for absorbance ScanDrop 06 2009 87 Expression Module Analytical Parameter Presettings Programming of Analytical Formula 9 3 2 Data Management Files with analytical results can be manipulated using the function keys in Expression module window Saving analytical results 1 Press Save key The standard window for file saving opens 2 Select path and file name and press OK for saving The default path for file saving in Expression module is StorageCard ScanDrop Bio sei is used as file extension Viewing the results of analysis 1 Press Open key The standard window for file opening opens 2 Select required sei file with analytical results and press OK for viewing Exporting the results of analysis 1 Press Export key The standard w
56. he key corresponding to that type Then press key AII The same sample type will be assigned to all test points on the active tab Note regarding sample assignments e Asample assignments chart need not necessarily begin with position 1 Unused channels of a tube may be assigned at a later point in time e A sample measurement sequence begins with the smallest assigned sample position e Areference must be placed before its related sample i e be defined with a position number that is smaller than the actual sample Defining sample properties in Quantification Bio method and Expression module The various samples in a given tube setup may be assigned to specific properties such as sample name sample type or correction factors e To open this input window press number key of the sample channel 2 in Fig 9 e Enter all required sample properties in this window and press OK to restore the tube setup window screen 34 06 2009 ScanDrop Quantitative Analysis Quantification Module 8 2 Quantitative Analysis Quantification Module The Quantification module is preferentially intended for express routine jobs of concentration analysis A concentration value is determined using the absorbance value obtained for a given wavelength Concentration analysis may be performed with a factor or based on a linear or square regression model alternatively Routinely a pre defined sequence of reference samples standard samples
57. ibration tab Only for concentration analysis with calibration Module QUANT Result list x Settings Samples Calibration Coefficients Both chanr gt 0123 45 6 7 8 mg ml Export Fig 16 Quant module Chip shows calibration graph Both calibration graphs are graphically represented on the Calibration tab The graph for the 1 mm layer thickness channel is marked with green color the graph for the 0 1 mm layer thickness channel in blue color Single samples can be selected from the samples list on the right hand side The absorbance and concentration values of a selected sample will be indicated numerically and marked with gray lines in the graphic field Coefficients tab Only for concentration analysis with calibration ScanDrop 06 2009 43 Quantitative Analysis Quantification Module Display amp Management of Measured Results Module QUANT Result list Settings Samples Calibration Coefficients Calibration Storage Regression 4 Bx Save Export New Close Fig 17 Quant module Chip shows calibration coefficients The Coefficients tab depicts the parameters for calibration In addition to the actual calibration coefficients it outputs the coefficient of determination and the values for maximum and minimum concentration Opening saving and exporting measured results Files with previously saved results of measurement are contained in a parameter window th
58. ical parameters Ca Note regarding assignment of reference and standard samples Measurement readings for reference samples and standard samples are always determined for both channels Defining sample properties On selection of the Sample prop tab the various samples of a sample assignments chart can be assigned a sample name standard concentration weighed in quantities and dilution factors in a sample table Module QUANT x 1 8 9 16 Sample Prop settings Load Sample names from file csv Pos Sample name Type Target a Reference Ref 2 Stand 1 Std 10 3 stand 2 std 20 j4 stand 3 std 30 5 stand 4 std 40 e Sample 1 Cone hd New Load Save Open Start Eject Default Close Fig 11 QUANT module Chip editing sample table 1 To enter or edit sample properties press corresponding sample line A window for defining the properties of a selected sample will open ScanDrop 06 2009 37 Quantitative Analysis Quantification Module Parameter Settings for Concentration Analysis Alternatively this window can also be opened from tabs 1 8 and 9 16 by pressing number keys 1 to 16 2 Available value entries Sample name Individual sample designation Type 0 1mm Sample type in shallow channel Type 1mm Sample type in deep channel Note For shallow channel and deep channel the same sample type or No property for sample type must be set References and
59. indow for file saving opens 2 Select path and file name and press OK for saving 9 3 3 Analytical Parameter Presettings Programming of Analytical Formula Analytical parameter presettings Working in the Presettings window you can define those analytical parameters which you want displayed on selection of the Expression module Besides this window also provides options for programming analytical formulas 1 Press Default key in Expression module window 2 Settings can be made for the following parameters Sample name Individualized sample name Expression Analytical formula Accumulations The number of repetitions of a single measurement cycle required to obtain a measured value Decimal places The number of decimal digits which are comprised in a result output Edit This key opens a list of formulas for programming a new expressions formula or deleting one or more previous formulas 88 06 2009 ScanDrop Expression Module Analytical Parameter Presettings Programming of Analytical Formula Confirm your entries with OK Following changes in parameter settings you will be queried if you want your new settings saved Acknowledge this query with Yes to validate your changes Compiling editing a formula Operation in Expression module relies on user programmed formulas for sample analysis The procedure for compilation or editing of formulas is similar to that for CHIPCUVETTE gt section Program
60. ined They should not be changed unless by prior consultation with Analytik Jena AG ScanDrop 06 2009 29 Operation Control with ASpect Nano General Settings for ASpect Nano Accumulation Defines the number of repetitions of a measurement cycle required to obtain a measured value This parameter can be changed in pre settings for the various modules and in any method Preset value 10 Flash number Number of flashes to expose the receiver line Preset value 3 Flash frequency Sets the number of flashes flash rate per second Preset value 4 Dark corr Records dark signal prior to each measurement if active Dark signal correction should always be active Data correction Corrects flash lamp intensity with the help of an internal reference channel The ScanDrop 250 includes two receivers for measurement in a CHIPCUVETTE and in a standard tube In the case of CHIPCUVETTE measurement the receiver for standard tube is used as reference channel and vice versa Wavelength indicates the midpoint value of a range that is selected for measurement of the flash intensity The size of this correction range can be defined in the Range input window It specifies the number of pixels to the right and to the left of Wavelength With check box Data smooth in active state all values obtained within the correction range will be subjected to smoothing In the case of ScanDrop 200 and ScanDrop 100 a photo diode functions as receiv
61. inetic Waiting time s 0 Time intervals 50 30 0 0 Time s 2 10 o o New Open Save Ok Fig 32 UVVIS module Chip parameter settings for cyclic measurement Kinetic slow and Kinetic fast For measurement in a CHIPCUVETTE two different kinetic modes are available Kinetic slow and Kinetic fast In kinetic fast mode measurement is completely performed at a given single test point before measurement of the next sample begins In terms of measurement kinetics this means that measurement for reaction kinetics will be fully performed on a given sample before the next measurement of reaction kinetics starts activate Start request check box As compared to this with extended kinetic measurement jobs you may optimize the total time to measure and perform measurement of samples in a cyclically timed manner Kinetic slow Within a given cycle one measurement is performed on each sample This is followed by measurement of the first sample as part of the next cycle Measurement continues in the same order until the last cycle has been completed Tabs for sample assignments on CHIPCUVETTE Settings for CHIPCUVETTE sample assignments are described in section Defining Sample Assignments for CHIPCUVETTE in ASpect Nano p 33 For measurement in UVVIS module the following sample options can be defined Ref Reference measurement Refrence measurement must always precede actual sample measurement
62. ings can be made for analytical parameters These will be displayed on selection of the Quant module 1 Press Default key in Quant module 2 Available parameter settings Default sample Type preset in CHIPCUVETTE setup type Decimal places Number of decimal digits for output of analytical results conc Wavelength Analytical wavelength Multiple meas Number of repetitions when a sample is measured for absorbance Concentration is determined from the mean value of all absorbance readings Unit conc Unit of measure for concentration analysis Accumulations The number of repetitions of measurement defined for determination of an absorbance value Concentration Selects type of concentration analysis allows entry of a factor or a calibration file name where required 3 Use OK to exit the Quant module Default settings window and confirm query for saving of changes with another OK All modified presettings will be saved and displayed when the Quantification module is selected for the next time ScanDrop 06 2009 45 Bio Method Module 8 3 Bio Method Module The Bio method module is intended for analysis of proteins DNA and RNA including related evaluation procedures according to these methods Name of method Formula DNA purity A260 A280 x A 260nm A 280nm Absorbance 260 nm x A 260nm Absorbance 260 nm factor 33 x ug ml A 260nm 33 Absorbance 260 nm factor 40 x ug ml
63. ion in rooms with an explosive atmosphere There must be no aggressive vapors for example strongly etching acidic or alkali fumes in the immediate surroundings of the system The designated site for installation must meet the following requirements e Absence of draught effects dust etching vapors and mechanical vibration e No electromagnetic fields e g motors located nearby e No dripping gushing or splashing water near the ScanDrop e No exposure to incident solar radiation or radiation emitted by heaters Temperature range 10 C 40C Max rel air humidity 93 at 40 C Footprint requirements With physical dimensions as quoted above and approximately 100 mm of clearance on all sides the ScanDrop requires a minimum footprint area of 500 mm x 450 mm W x D ScanDrop 06 2009 13 Transportation amp Installation Requirements 14 06 2009 ScanDrop Installation amp Start Up Standard Delivery Scope Installation amp Start Up Standard Delivery Scope ScanDrop delivery may include system versions and accessory items as specified hereafter ScanDrop 100 e ScanDrop 100 spectrophotometer for 10mm standard tubes e ASpect Nano software e 1 standard glass tube 10 mm layer thickness e 1 standard quartz tube 10 mm layer thickness ScanDrop 200 e ScanDrop 200 spectrophotometer for CHIPCUVETTE e ASpect Nano software e 2CHIPCUVETTE micro trubes ScanDrop 250 e ScanDrop 250 spectrophotome
64. ions This key opens a formula list for programming new formulas or deleting prior formulas Acknowledge your entries with OK Following changes in parameter settings you will be queried if your settings are to be saved Confirm this query with Yes in order to validate the changes Programming a formula Analysis performed via the Expression module relies on user compiled formulas for evaluation of samples The absorbance values of a maximum of six wavelengths may be included in programming A formula can be compiled starting from the Default settings window 62 06 2009 ScanDrop Expression Module Analytical Parameter Presettings Programming of Analytical Formula 1 Press Default key in parameter window of Expression module 2 Press Edit formula key This produces a formula list Press Add in formula list 3 Set number and related values of analytical wavelengths in Set wavelengths window O Set Wave lengths to use for expression Use or key of Number field as necessary to set a required number of wavelengths x 1 6 Keys A1 to Ax will turn active according to your settings Ax will designate the absorbance value for a specified wavelength in the resulting formula Press A1 key and enter first wavelength in numerical field that has opened Proceed in the same manner until all wavelengths have been defined Specify a unit of measure for analysis at the Unit box This entr
65. is largely maintenance free To maintain your laboratory certification and equipment validation state we offer a maintenance agreement with IQ OQ installation and operation qualification Required action for preventive maintenance and care on your part is restricted to cleaning of the system enclosure and its display screen In the event of maloperation of defects in the system you are requested to contact our Customer Service department You can find its address details on the inside of the title page 10 1 Cleaning the ScanDrop Sample material spillings in the sample compartment or on surfaces of accesory items should be wiped off with blotting paper immediately Use a piece of soft clean cloth which can be moderately wetted with a commercial neutral detergent to remove soiling or pockets of contamination from system surfaces Cleaning in a medical laboratory Special care should be used where the ScanDrop is required to perform analysis of infectious material because ScanDrop cannot be decontaminated as a whole Perceivable contamination must be immediately removed with suitable aids Make sure that no solvent can penetrate into the inner system space as you do this Recommended disinfectants Decosept AF Spray disinfectant from Dr Schuhmacher GmbH Meliseptol HBV cloth from B Braun Use a non shedding piece of cloth to wipe the system and adapter surfaces down for decontamination Make sure that affixed lettering is not w
66. ition of sample sequence via analytical Parameters ceeste neren ier Esi E RERE ark ESE OKEE AEREE ONAE 37 Fig 11 QUANT module Chip editing sample table eee 37 Fig 12 QUANT module Chip measurement parameter settings 38 Fig 13 QUANT module Chip display of selected analytical parameter SOtUuNGS sss cleat kei dhe ei Aha eee at elise Sia a 41 Fig 14 Quant module Chip outputs table with results of analysis 41 Fig 15 Quant module Chip shows sample properties and single FOACINGS ie oeei hee atid ET E E he cee 43 Fig 16 Quant module Chip shows calibration graph cceeeee 43 Fig 17 Quant module Chip shows calibration coefficients 44 Fig 18 Bio module Chip parameter WiINdOW ccecceeeeeeetteeeeteeees 47 Fig 19 Bio module Chip sample sequence settings cseeeeeeees 48 Fig 20 Bio module Chip for configuration or modification of sample tables ei abit cea tie ude antes Lahde tiniest 49 Fig 21 Bio module Chip general analytical parameter settings 50 Fig 22 Bio method Chip display of results liSt 0 ccecceeeeeeeeereeees 52 Fig 23 Bio module Chip displays result of a single sample 53 Fig 24 Expression module Chip parameter window 0 ceseee 55 Fig 25 Expression module Chip sample sequence settings 57 Fig 26 Expressi
67. ized sample name This entry is optional Method Provides a selection list of available analytical methods Select a desired bio method ScanDrop 06 2009 83 Bio Method Module Management of Analytical Results in Bio Mehod Module Accumulation Specifies the number of single measurement repetitions you want included in the formation of a measured value All single measurement readings will be averaged and output and the result of measurement absorbance Accumulation of the individual readings helps improve the signal to noise ratio Place reference sample into sample chimney 2 3 Trigger reference measurement by pressing Ref key 4 Place tube with analytical sample into tube chimney 5 Trigger measurement by pressing Start key Measurement starts with predefined parameter settings Module Bio x Sample name ficcrabbt Methode DNA Purity 260 280 X Accumulations 10 Result Corr 320 nm Measurement values nm 4 0 4186 280 0 6206 320 0 3172 Operator New Open Save Export Ref Start Default Close Fig 42 Bio module Std display of results The results of analysis are displayed in the lower half area of the Bio module window In addition to the calculated results it outputs the values for absorbance Background correction is performed if the Corr 320 nm check box was set The absorbance value obtained at 320 nm is subtracted from the aborbance values that have bee
68. key in parameter window 2 Select cqu file and press OK for acknowledgement The analytical parameters are displayed in the parameter window They can be edited or used for subsequent measurement 8 2 2 Triggering Concentration Measurement 1 Pprepare parameters for concentration analysis 2 Load CHIPCUVETTE in accordance with the sample setup as made via measurement parameter settings and place tube into holder frame 3 Press Start to start measurement Measurement begins with predefined measurement parameter settings Samples are automatically evaluated in accordance with the selected sample setup 8 2 3 Display amp Management of Measured Results On completion of measurement the results are displayed on the four tabs Settings Samples Calibration and Coefficient of the results window The available function keys are intended for saving and exporting the results of measurement and for returning to the parameter window 40 06 2009 ScanDrop Quantitative Analysis Quantification Module Display amp Management of Measured Results Tabs in results window Settings tab Module QUANT Result list Settings Samples Calibration Coefficients Title Operator Administrator Wavelength 280 Unit mg ml Multi measure 1 Calibration IgGKalib cqu Y A BX Save Export New Close Fig 13 QUANT module Chip display of selected analytical parameter settings The following valu
69. luates samples analyzed for concentration Bio method Measures proteins DNA and RNA with respective evaluation Expression Performs evaluation based on user defined formulas UV VIS Measures sample intensities and outputs results for transmittance absorbance and energy Actions for system operation control and evaluation of results are normally triggered at the large touch sensitive LCD monitor touch screen of HID Pro 320 Its input fields are adequately sized to allow operation control with one s fingers A commercial palm pen can also be used as input aid A key control button option or control field can be activated by pressing it For inputs in an entry field an alpha numerical or numerical keypad will show as soon as the input field is active Inputs with the alpha numerical keypad will not become valid unless confirmed by pressing OK Numerical values on the numerical keypad can be acknowledged by pressing lt The main menu will be displayed when a ScanDrop session starts It contains a tool for selection of a tube keys for triggering the various analytical modules and one key to open the system settings window The starting window of ASpect Nano software can be modified For example required analytical module windows can be directly opened or a menu for method selection be chosen in addition to the main menu refer to section Setting Initial Screen After ScanDrop Power Becomes Available p 28 Selec
70. measurements to obtain an absorbance value Decimals places The number of decimal digits for display of results Unit The unit of measure in which analytical results will be shown Correction 320 nm Activates background correction at 320 nm 3 To exit the Bio Module Default settings module press OK Then use another OK to confirm that you want your changes saved Modified presettings will be saved and available on display when a bio module session is selected for the next time 54 06 2009 ScanDrop Expression Module 8 4 Expression Module The Expression module allows you to link as many as six absorbance values obtained at different wavelengths in a single mathematical formula Automatic determination of optimal measuring range Similar to the procedure followed in Quantification module a measurement job involving both test points of a sample channel will consider only that measured reading for analysis which is within a linear measuring range of 0 005 to 1 5 Abs If both absorbance values are within this area the one which is closer to 0 75 Abs will be included Measured values are checked for accuracy according to these criteria e Abs 1mm gt Abs 0 1 mm e Abs 1mm gt 0 005 e Abs 0 1 mm lt 1 5 Abs 1 mm Absorbance value for 1 mm layer thickness Abs 0 1 mm Absorbance value for 0 1 mm layer thickness If these requirements are not fulfilled the particular measured value will be marked as faulty wi
71. ment cannot always be guaranteed To enable users to account for the precise value of a layer thickness in analytical processes system delivery includes a correction factor for each lot of tubes This factor normalizes the result of measurement or analysis to a layer thickness of 100 um in all cases It must be entered at the Correction factor of channels input field Position testing of CHIPCUVETTE A CHIPCUVETTE must always be inserted in a correct position in relation to the tube holder see Fig 5 S 23 If the CHIPCUVETTE is installed in a rotationally twisted position its samples will be mal assigned If the Check position of inserted CHIPCUVETTE check box is active the CHIPCUVETTE will automatically be checked for correct placement in its holder frame Note To check for correct ordinate location and correct wavelength with filter chip position testing must be turned off ScanDrop 06 2009 31 Operation Control with ASpect Nano Data Management on HID Pro 320 7 2 Data Management on HID Pro 320 Files must always be saved on the memory card Storage Card folder The HID Pro 320 operation control unit does not provide enough memory space for this purpose Analytical data are managed and maintained in individual analysis modules File summary table in Aspect Nano File name File content Quantification modules cqu Analytical parameters and results of concen
72. ming a formula p 62 Once compiled or edited a given formula can equally be used for measurement in a CHIPCUVETTE or a standard tube ScanDrop 06 2009 89 User Defined Measurement UV VIS Module 9 4 User Defined Measurement UV VIS Module Sample intensities can be measured with the help of the UV VIS module Results can be output in units of transmittance absorbance or energy Subject to measurement can be spectrums values at discrete wavelengths or values at two wavelengths with the pertaining differential amount Furthermore multiple measurement is possible for purposes of kinetic analysis However PC software is required for evaluation in this case Where standard tubes are used only single samples can be measured For further processing of a single reading the particular analytical value must be saved in a separate file after each sample measurement Triggering UV VIS module 1 For ScanDrop 250 Select Standard 10 mm tube type in main window 2 Press UV VIS key The UV VIS module window opens Module U IS Chart Table Settings Para Open Save Export Ref Start Fig 45 UV VIS module Std starting screen Function elements in UV VIS module window Three tabs Chart Table and Settings are available for presentation of results They are explained in section Display amp Management of Measured Results p 94 The module s function keys are located at
73. module Std display of results cccceeeeeeesseeeeeseeeeeeeees 84 Fig 43 Expression module Std parameter settingS 0 c ceeeee 86 Fig 44 Expression module Std display of resultS cceeeeeee 87 Fig 45 UV VIS module Std starting Screen 90 Fig 46 UV VIS module Std parameter SettingS ccccsceeeeeereeees 91 Fig 47 UVVIS module Std display of spectrum s s s 94 104 06 2009 ScanDrop
74. module window is intended for selection of measuring parameters and an analytical formula as well as display of analytical results Its function keys are located at the bottom bar of the dialog screen area Function Description New Deletes all previous entries and allows new entries for analytical parameters Open Opens analytical file Save Saves analytical results Export Exports analytical results to a text file Ref Performs reference measurement Start Triggers measurement with specified analytical parameter settings Default Allows programming of analytical formula and definition of presettings Close Closes the Expression module 86 06 2009 ScanDrop Expression Module Triggering Measurement amp Displaying Analytical Results 9 3 1 Triggering Measurement amp Displaying Analytical Results 1 Prepare analytical parameters Make settings for the following parameters Sample name Individualized sample name This entry is optional Accumulation Specifies the number of repetitions of single measurement you want performed for calculation of a measured value All single measurement readings will be averaged and output as the result of measurement absorbance Accumulation of single readings helps improve the signal to noise ratio Formula Provides a list of choices to select from available analytical formulas Select desired formula Place reference sample into sample chimney 2 3 Press Ref key to tri
75. mp Managing Analytical Results Module Expression result list x Results Settings Pos Sample name Expression Result Probe 1 Reference 2 Probe 2 A1 1 45 a2 0 74 1 0592 3 Probe 3 A1 1 45 A2 0 74 1 0732 4 Probe 4 A1 1 45 a2 0 74 0 9317 5 Probes A1 1 45 A2 0 74 07813 je Probe 6 A1 1 45 A2 0 74 0 6551 z Probe 7 A1 1 45 A2 0 74 052 Is Probes A1 145 A2 0 74 04339 Save Export New Close Fig 27 Expression module Chip display of results list Tabs in results window Results tab Results contains a sample table with a summary of the results of measurement It displays the following values Pos Sample name Expression Result Position number of sample on CHIPCUVETTE Sample name defined via sample properties prior to measurement Analytical formula which is applied to the sample Result of analysis A Sampe result window with more sample details will open when a given sample line is clicked Name Type Position Date Time Result Table Sample name Analytical formula which was applied to the sample Position of this sample on CHIPCUVETTE Time of analysise Result of analysis Values measured for absorbance For CHIPCUVETTE both readings one for 0 1 mm test point and the other for 1 mm test point are indicated 60 06 2009 ScanDrop Expression Module Displaying amp Managing Analytical Results Module Expression sample re
76. n measured for a given bio method 9 2 2 Management of Analytical Results in Bio Mehod Module Files with analytical results can be managed using the function keys in Bio module window The default path for file saving in Bio method module has been assigned to StorageCard ScanDrop Bio The selected file extension is sbi Opening analytical file 1 Press Open key The standard window for file opening opens 84 06 2009 ScanDrop Bio Method Module Presettings in Bio Method Module 2 Select sbi file with the analytical results of a bio method and press OK to open this file Note Once a result file has been loaded its prior method selection can be used for further measurement Saving measured results 1 Press Save key The standard window for file saving opens 2 Select path and file name and press OK to save this file Exporting results of measurement Data can be exported to a text file where they will be available for further processing in other programs for example Excel 1 Press Export key in result window The standard window for file saving opens 2 Select path and file name and press OK for saving 9 2 3 Presettings in Bio Method Module For analytical parameters it is possible to make presettings These will be loaded when a Bio method module session starts 1 Press Default key in Bio module window 2 Make parameter settings for Sample name Individualized
77. n or removal of a tube of this type Default Compiles formulas for use in analytical processes Defines presettings These are displayed at the beginning of a new analytical procedure Close Closes the Expression module 8 4 1 Settings for amp Management of Analytical Parameters On selection of a particular formula the required relevant measurement parameter settings will be loaded automatically Accordingly only a formula needs to be selected and sample assignments be defined for CHIPCUVETTE Defining sample sequence Action to make settings for CHIPCUVETTE sample assignments is described in section Defining Sample Assignments for CHIPCUVETTE in ASpect Nano p 33 56 06 2009 ScanDrop Expression Module Settings for amp Management of Analytical Parameters Module Expression 1 8 Jo 16 Sample Prop Settings Expr y ma ml 4 280 1 45 A 260 0 74 rf 1 2 3 4 5s oe 7 2 TRI Load Save Open Start Eject Default Close Fig 25 Expression module Chip sample sequence settings For measurement in Expression module the following sample types can be defined via tabs 1 8 and 9 16 Expr Sample being analyzed according to selected formula Ref Reference Note Reference measurement must always precede sample measurement No Deletes single sample channels AII Assigns sample type selected above to all sample channels
78. ng 3 Mark a desired file and press OK for acknowledgement The default setting for saving the results files of concentration analysis is StorageCard Scandrop Quant The file extension is cqu Calibration data are loaded from the available file and will be available for subsequent concentration analysis Note When working with a previously saved calibration graph you are not able to measure with other standards nor include these for calibration ScanDrop 06 2009 39 Quantitative Analysis Quantification Module Display amp Management of Measured Results Saving and loading analytical parameters Analytical parameter settings can be managed with the help of the two keys Load and Save in the parameter window The default path for saving files in Quantification module is StorageCard ScanDrop Quant The file extension is cqu Saving an analytical parameter file 1 Set analytical parameters you want saved in the parameter window 2 Press Save key The standard window for file saving opens 3 Set path name and file and press OK for acknowledgement Loading analytical parameters Analytical parameters and measured results are saved in the same manner A file always contains analytical parameter settings A result file contains the absorbance values for the measured samples in addition Accordingly analytical parameters can be loaded from a parameter file or a result file 1 Press Load
79. nit ScanDrop 06 2009 17 Installation amp Start Up Components amp Terminals Rear side connection terminals Eo 6 5 4 3 2 1 RS 232 interface port Terminals for water supply to heat exchanger and thermostat with temperature controlled standard tube Electric connection points for Peltier thermo controller and standard tube USB port PC Terminal for external power pack N Terminal for standard tube stirrer On Off switch N OO A O Fig 3 Connector terminals on the rear side of the ScanDrop 18 06 2009 ScanDrop Installation amp Start Up Connecting the ScanDrop 5 3 Connecting the ScanDrop 1 Remove the ScanDrop power pack operation control unit and user manual from their transport packaging Allow adequate time for the system to thermally adjust to its ambient temperature Store transport packaging for future transportation if necessary Mount HID Pro 320 operation control unit onto the mechanical interface at the top of the ScanDrop The operation control unit is held in position by magnets Alternatively you may connect a PC in order to perform operation control and data evaluation of the ScanDrop at the PC Connect the ScanDrop with the power pack and connect power pack to line power supply 5 4 Starting Up amp Turning On A Caution Liquids Do not place objects especially liquid containing vessels onto the ScanDrop Refrain from tilting The operation control uni
80. on Working with the ScanDrop p 5 e Work steps are numbered This Manual contains a description of ScanDrop 100 ScanDrop 200 and ScanDrop 250 product models A description part which equally applies to all three product models will provide collective references to the ScanDrop as such Where a particular feature of a given product model is to be specifically highlighted the full product name will be quoted 06 2009 ScanDrop Safety Instructions General Safety Instructions 2 2 1 Safety Instructions General Safety Instructions For your own safety and to assure failsafe operation you should carefully read this chapter before you proceed with any kind of ScanDrop start up action Comply with all safety requirements that are contained in this Manual and follow all messages and prompts that are displayed on the monitor screen by control software You are also advised to observe all safety requirements for integrated system components from other manufacturers e g operation control unit printer Remember to comply with safety advice on labels and specifications regarding the handling storage and disposal of reagents reaction tubes and accessory pack slips of test sample sets Intended use The ScanDrop including any original accessories may not be used for applications other than described in this User Manual No manufacturer warranty can be assumed in the event of any other type of use including that of in
81. on module Chip definition or modification of sample TADIO LOTEEN cubs EEA EEEE ATE EE OET T ae 58 Fig 27 Expression module Chip display of results list 60 Fig 28 Expression module Chip single result display for a sample 61 Fig 29 Expression module Chip defining wavelengths for formula 63 Fig 30 Expression module Chip editing of formulas seeee 64 ScanDrop 06 2009 103 List of Illustrations Fig 31 UVVIS module Chip parameter settings for wavelengths 67 Fig 32 UVVIS module Chip parameter settings for cyclic measurement be stica hed sy A AEA E A AT 68 Fig 33 UVVIS module Chip parameter settings with sample seguen Ns iii etal iain ie lan ne ela 69 Fig 34 UVVIS module Chip graphic representation of spectrum 70 Fig 35 UVVIS module Chip samples table cceecceeeeeeeeeeeeeneees 71 Fig 36 Quant module Stand calibration parameter settings 74 Fig 37 Quant module Stand definition of sample table 0 75 Fig 38 Quant module Stand display of calibration results 78 Fig 39 QUANT module Std display of results list cece 78 Fig 40 QUANT module Sid view of parameter settings for current analytiGal Joba eitie taisni nadraai at edoae aaea Sa anand aKa 79 Fig 41 Bio module Std parameter and results window see 83 Fig 42 Bio
82. oncentration specified via analytical parameters Type Sample type standard concentration value or absorbance value Dilution Dilution factor Init weight Concentration factor Active Includes the entire sample into calculation procedures e g for determination of mean value or calibration parameters if active Does not consider a given sample for calculation if inactive An inactive sample value is marked with a bracket in the sample table Table Values measured for absorbance For CHIPCUVETTE mode both measured readings are displayed i e that from the 0 1 mm deep test point the other from the 1 mm deep test point With multiple measurement sequences all single sample readings are indicated Related mean values are output below the table Single sample values can be deactivated in the table To do this touch the particular single sample value and acknowledge the Do you want to deactivate measured value query with OK A deactivated single sample will be marked with a bracket in the table The related mean value will change correspondingly 42 06 2009 ScanDrop Quantitative Analysis Quantification Module Display amp Management of Measured Results Module QUANT Sample result Name Concentration stdi 8 2969 Type Concentration o tmm fimm f Dilution 1 0 1233 1 1956 Init weight 1 Active Iv 0 1233 1 1956 Close Fig 15 Quant module Chip shows sample properties and single readings Cal
83. op 06 2009 61 Expression Module Analytical Parameter Presettings Programming of Analytical Formula Closing the results window The results window can be exited by clicking the New or the Close control button Following a query if unsaved data are to be saved the Expression module parameter window is restored New Parameter window displays basic settings or presettings Close Parameter window preserves the parameter settings of a preceding measurement They can be selected or modified for use in next measurement 8 4 4 Analytical Parameter Presettings Programming of Analytical Formula Analytical parameter presettings Working in the Default settings window one can enter analytical parameter settings that will be displayed on selection of the Expression module In addition this window also provides options for programming of analytical formulas 1 Press Default key in parameter window 2 Available value setting options are Expression Analytical formula Sample type The sample type that is valid for all sample positions in a sample setup Accumulations Defines number of repeat cycles of a single measurement From the total number of single measurements mean readings are determined for each selected wavelength and output as results Accumulation of single measurement readings helps improve the signal to noise ratio Decimal places Number of decimal digits contained in a result output Edit Express
84. r settings for concentration analysis Saves currently valid analytical parameters Opens previously saved result file of prior concentration analysis Triggers measurement with analytical parameters as user set Moves holder frame of CHIPCUVETTE out for insertion or removal of a tube of this type Defines presettings that are displayed prior to each new concentration analysis Closes the Quantification module 8 2 1 Parameter Settings for Concentration Analysis Entering a sample sequence Action for sample assignment settings in CHIPCUVETTE mode is described in section Defining Sample Assignments for CHIPCUVETTE in ASpect Nano p 33 For measurement in Quantification module the following sample types can be defined via tabs 1 8 and 9 16 Ref Reference Note Reference measurement must always precede a standard or a sample measurement 36 06 2009 ScanDrop Quantitative Analysis Quantification Module Parameter Settings for Concentration Analysis Std Standard with known analyte concentration Conc Sample being measured for concentration Abs Sample being measured for absorbance only No Deletes individual sample position s All Assigns previously selected sample type to all sample channels Module QUANT 1 8 Jo 16 Sample Prop Settings Load Save Open Start Eject Default Close Fig 10 Quant module Chip for definition of sample sequence via analyt
85. ration The purpose of the Calibration tab is to present a graphical view of the calibration curve Single samples can be selected from the sample list on the right hand side The absorbance and concentration values of a selected sample are displayed in numerical format and marked with red lines in the graphical screen area By pressing the Detail key you can open the Sample result window for a selected sample see Table tab below ScanDrop 06 2009 77 Quantitative Analysis Quantification Module Display amp Management of the Results of Concentration Analysis Module QUANT Result list x Calibration Table Settings conc 60 6 4 0 6154 0 25 50 75 100 125 Detail Save Export New Close Fig 38 Quant module Stand display of calibration results Table tab Table contains a sample table with a summary of analytical results Pos Number of sample in sample table Sample name Sample name defined via sample properties prior to measurement Type Sample type reference standard concentration value or absorbance value Conc Calculated concentration value Module QUANT Result list x Calibration Table settings Pos Sample name Type conc 2 fsa STD 65 3 stez STD 22 a afsta STD 606 5 std4 STD 125 je stds STD 158 Z pi CONC 158 3653 e pr2 CONC 61 2088 a pr3 CONC 22 0221 xl Save Export New Close Fig 39 QUANT module Std display of results list On selection of a pa
86. regarding ScanDrop 250 The CHIPCUVETTE holder must have been fully lowered into the ScanDrop Otherwise its cover will obstruct access to the standard tube chimney 1 For ScanDrop 250 Select Standard 10 mm option from the Select measurement list in the main menu 2 Fill sample into the tube minimum fill height 17 mm 1 7 mL 3 Place tube into the tube chimney The arrow beside the tube chimney indicates the direction of beam passage The tube s optical blank surfaces must be orthogonal in relation to the beam ScanDrop 06 2009 23 Inserting Samples Working with a Standard Tube 1 Standard tube 2 Arrow marker for measuring beam direction 2 1 Fig 6 Standard tube installed in measuring chimney LF Note regarding ScanDrop 250 Always remove the CHIPCUVETTE from the ScanDrop before you begin measurement with a standard tube During measurement of a standard tube the measurement channel of CHIPCUVETTE is used as internal reference A CHIPCUVETTE present in the beam path may falsify the measured result 24 06 2009 ScanDrop Operation Control with ASpect Nano 7 Operation Control with ASpect Nano When you work with the HID Pro 320 control unit system operation is controlled and data packages are evaluated by ASpect Nano software which has been specifically developed for biochemical analysis ASpect Nano software is of modular setup It consists of the following modules Quant Eva
87. rticular sample line the Single sample result window with more sample related details will open Name Sample name Concentration Concentration value Target value Only for standards Nominal concentration as defined via analytical parameters Type Sample type standard concentration value or absorbance value Dilution Dilution factor 78 06 2009 ScanDrop Quantitative Analysis Quantification Module Display amp Management of the Results of Concentration Analysis Init weight Concentration factor Active Includes sample in procedures for averaging or calculation of calibration parameters if active The given sample will not be considered for calculation is this button is disabled A disabled sample value will be marked with parenthesis in the sample table Measured valuesThe reading obtained for absorbance or the individual absorbance readings in the case of multiple measurement Mean values are output below the table Single sample values can be disabled in the table To do this tap on a desired single sample value and cofirm Do you want measured value disabled query with OK Once disabled the single sample in question will be marked with parenthesis in the table The related mean value will change accordingly Settings tab The following items of analytical parameter settings are displayed and saved together with the results of measurement Title Title of measurement Operator Name of user Wavelength An
88. sample name Method Selects bio method Accumulation Defines the number of repetitions of single measurement you want included in the calculation of a mean value Decimal places The number of decimal digits in a result output Correction 320 nm For activation of background correction at 320 nm 3 To exit the Bio module Default settings window use OK Then press OK once again to confirm you want your changes saved Modified presettings will be saved and output for display when the Bio module is selected the next time ScanDrop 06 2009 85 Expression Module 9 3 Expression Module The Expression module allows linking a maximum of six absorbance values obtained at different wavelengths in a mathematical formula Only single measurement is possible when working with standard tubes Where further processing is required the given analytical value must be saved ina separate file after each sample measurement Triggering Expression module 1 For ScanDrop 250 Select Standard 10 mm tube type in main window 2 Press Expression key The Expression module parameter window opens Module Expression x Sampe name Expression y Abs A 260 4 280 T Accumulations Mi Result Measurement values nm A nm A o 2500 Operator New Open Save Export Ref Start Default Close Fig 43 Expression module Std parameter settings Function elements in Expression module window The Expression
89. sion Mod le sisisi inisini iani ae dai Na 55 Settings for amp Management of Analytical Parameters 0 c0 56 Triggering Measurment ee eeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeteeeeeeeeeeeeaeeeeeeeaeees 59 Displaying amp Managing Analytical Results eee eeeeeeeeeeeeeees 59 Analytical Parameter Presettings Programming of Analytical FOrmula cccccccecececeesceeeeeeeceeeeeaeeeeaeeseeeeeseeeeesaeeeeeeeee 62 User Defined Measurement UV VIS Module cceeseeeereees 65 Setting amp Managing Measurement ParameterS c0cccccssseeeeeees 65 Performing Sample Measurement cccccccesesecceeesseseeeeseeeeessseaees 70 Display amp Management of Measured Results cccceeeeseeeereees 70 Measurement in Standard Tube ccccccesseeeeseeeeees 73 Quantitative Analysis Quantification MOduUIe cccccesseeeeeees 73 Analytical Parameter Settings ccccececeecseeeeeeeeeeeeeeseeeeeseaaeseeneeee 74 Triggering Concentration Analysis cccccceceeeseeceeeeeeeteeeeeeeeeeeeetas 77 Display amp Management of the Results of Concentration Analysis 77 Presettings in Quantification Module 0 cceeeeceeeeeeeeeeteeeeteeteeeeees 80 Bio Method Module cccceceeeeeeeeneeceeeeeeeeseeenaeeeeeeeeeseenaneeeeeeeeess 82 Selection of Bio Method amp Triggering Analysis c ccseeeeeeeeeees 83 Management of Analytical Results in
90. sult Name Probe 3 Type y majml A 280 1 45 A 260 0 74 Position 3 Date Time 1 2494 0 1041 0 9978 Result 1 0732 Used channel 1 mm Fig 28 Expression module Chip single result display for a sample Settings tab The Settings tab indicates the Title the Operator and the number of Accumulations of an analytical job This data is saved together with the results of measurement Opening saving and exporting measured results Files with previously saved results of measurement can be opened in the parameter window that appears following selection of the Expression module The default path for file saving is StorageCard ScanDrop EXPRESSION The file extension is cbi 1 Press Open key in parameter window The standard window for file opening opens 2 Select cei file with analytical results from Expression module and press OK for acknowledgement Measured results can be saved in the results window 1 Press Save key in result window The standard window for file saving opens 2 Select path and file name and press OK for saving Data can be exported to a text file where they will be available for further processing in other programs for example Excel Files can be exported working in the result window 1 Press Export key in results window The standard window for file saving opens 2 Select path and file name and press OK for saving ScanDr
91. t just sits on the connector interface of the basic unit It may fall off if the system is tilted up Use the On Off switch to turn the ScanDrop on The ScanDrop will automatically undergo an initialization routine The screen for password entry appears on the monitor Enter your user name with related password if there is an installed user identity management system Otherwise make these entries User name Administrator Password admin The main menu screen will show The ScanDrop is ready for measurement ScanDrop 06 2009 19 Installation amp Start Up Turning Off 5 5 Turning Off 1 Remove samples Remove standard tube from tube chimney Trigger CHIP to move the CHIPCUVETTE holder frame out of the ScanDrop and remove the CHIPCUVETTE 2 Turn power off using the ScanDrop On Off switch 20 06 2009 ScanDrop Inserting Samples Working with a CHIPCUVETTE 6 6 1 Inserting Samples Working with a CHIPCUVETTE Only for ScanDrop 250 and ScanDrop 200 Structure of a CHIPCUVETTE A CHIPCUVETTE can be loaded with as many as sixteen samples All sample channels are of through single space type so they can be filled without formation of bubbles Each channel has two test points a shallow point with a layer thickness of 0 1 mm and a deeper one with a layer thickness of 1 mm A channel has two openings for sample filling from a pipette The opening on the shallow channel side can be serv
92. t measurement x CHIPCUVETTE Module Quantification Bio methods Expression Device Settings Fig 7 Main menu of ASpect Nano software Operator action for the analytical modules is different for working with a CHIPCUVETTE and with a standard tube For this reason measurement with the two types of tube will be discussed separately in this User Manual General ScanDrop 06 2009 25 Operation Control with ASpect Nano General Settings for ASpect Nano system settings such as language user identity management or starting options for ASpect Nano are described in section General Settings for ASpect Nano p 26 Abbreviations for convenience This ASpect Nano software description uses Chip for CHIPCUVETTE and Std for a standard tube 7 1 General Settings for ASpect Nano For access to general settings options press the Settings key in the main menu The following system parameters and properties can then be configured e User identity management e Language setting for software user screen e Settings for starting menu after power becomes available to the ScanDrop e General measurement parameter settings e Software updates e Alignment of CHIPCUVETTE The bigger part of setting options is only available to persons with administrator rights 7 1 1 User Identity Management amp Current User Log Off Cr Note In initial setting state a user with administrator rights is logged on User name A
93. ter for CHIPCUVETTE and 10 mm standard tubes e ASpect Nano software e 2 CHIPCUVETTE micro tubes e 1 standard glass tube 10 mm layer thickness e 1 standard quartz tube 10 mm layer thickness Optional components e HID Pro 320 portable control unit e Set of CHIPCUVETTE 5 tubes e Set of CHIPCUVETTE 25 tubes e Stirrer for ScanDrop 250 and ScanDrop 100 e Water or Peltier temperature controller for ScanDrop 250 and ScanDrop 100 ScanDrop 06 2009 15 Installation amp Start Up Components amp Terminals 5 2 Components amp Terminals The ScanDrop is of modular setup It consists of a basic system unit that can be combined with a HID Pro 320 operation control unit Once mounted onto the interface port at the top of the basic unit the control unit will be operational ScanDrop analytikjena HID Pro 320 operation control unit Basic ScanDrop unit Chimney for 10 mm layer thickness standard tubes ScanDrop 250 and ScanDrop 100 Receptacle for CHIPCUVETTE micro tubes closed A UO N Fig 1 General view of ScanDrop 16 06 2009 ScanDrop Installation amp Start Up Components amp Terminals Connector terminals at HID Pro 320 control unit The HID Pro 320 control unit provides a USB port for data transmission It is required for software updates and to export measurement and evaluation data 2 1 USB port 2 0 2 LAN port Fig 2 Connector terminals at HID Pro 320 control u
94. th a supporting reference note to non conforming sample preparation Triggering Expression module 1 For ScanDrop 250 Select CHIPCUVETTE tube type in main window 2 Press Expression key The Expression module parameter window opens Module Expression x 1 8 Jo 16 Sample Prop Settings All New Load Save Open Start Eject Default Close Fig 24 Expression module Chip parameter window ScanDrop 06 2009 55 Expression Module Settings for amp Management of Analytical Parameters Function elements in Expression module parameter window The Expression module parameter window provides four tabs 1 8 and Tube setup with sample assignments for CHIPCUVETTE 9 16 Sample Prop Sample table with details regarding sampling sequence and analytical formulas Settings Input of measurement parameters that are independent of the analytical formula Its function keys are located at the bottom bar of the dialog screen Function Description New Deletes all previous entries and allows new settings for analytical parameters Load Loads previously saved measuring and analytical parameters for analysis Save Saves current measurement and evaluation settings Open Opens previously saved file with analytical results Start Triggers measurement with selected settings for measurement parameters Eject Moves holder frame of CHIPCUVETTE out for insertio
95. the Quant module allows users to prepare and save methods to such a degree that an analytical process just neeed sto be triggered via Start once a method has been selected and relevant sample assignment been made For routine analysis in standard tubes it is necessary to prepare a sample table that specifies a given sample sequence along with sample properties Triggering Quantification module 1 For Scandrop 250 Select Standard 10 mm tube type in main window 2 Press Quantification key The Quant module parameter window opens Function elements in Quant module parameter window The Quant module window provides two tabs General For input of measurement and evaluation parameters required in concentration analysis Sample table Table of samples with details regarding the sample sequence sample type name and standard concentrations The relevant function keys are located in the bottom bar of this dialog window Function Description New Deletes all previous input and allows new analytical parameter settings Save Saves currently valid analytical parameter settings Open Opens a result file of concentration analysis Presents analytical results in the results window Load Loads previously saved analytical parameters for use in other measurement jobs Start Triggers measurement with measurement parameters currently set Default Defines those presettings which will be displayed at the beginning of a ne
96. the broad bar the deep test point end This tube setup is identical for all measuring modules Module QUANT 1 8 ae 2 Sample Prop mas Load Save Open Start Eject Default Close 1 Keys for selection of sample type 2 Number keys of sample channels 3 Keys for sample channels Fig 9 QUANT module window sample assignment settings for CHIPCUVETTE The keys 1 in Fig 9 on the left of the actual tube setup area are intended for assigning a required sample type also refer to description of analysis modules An activated sample type will be assigned on marking of a test point Sample type Depending on the selected analysis module the appropriate sample types will be enabled for definition reference standard concentration analysis sample etc No Removes marker from a test point AII Assigns marked sample type to all test points Removes all markers if No is active e To assign a sample type to a test point activate its related key for example Conc Then touch a sample channel 2 in Fig 9 A sample channel is fully marked in color e To deactivate just one test point activate key No at first Then touch all those test points you do not want to measure ScanDrop 06 2009 33 Measurement in CHIPCUVETTE Defining Sample Assignments for CHIPCUVETTE in ASpect Nano The markers at these test points will be removed e Toassign the same sample type to all test points activate t
97. the next selection of the Quantification module ScanDrop 06 2009 81 Bio Method Module Bio Method Module The Bio method module facilitates measurement of proteins DNA and RNA with related evaluation procedures according to the methods listed hereafter Name of method Formula DNA Purity A260 A280 x A 260nm A 280nm Absorbance 260 nm x A 260nm Absorbance 260 nm factor 33 x ug ml A 260nm 33 Absorbance 260 nm factor 40 x ug ml A 260nm 40 Absorbance 260 nm factor 50 x ug ml A 260nm 50 Absorbance 280 nm x A 280nm Absorbance 280 nm factor x mg ml A 280 nm 1 38 1 38 Kalb Bernlor x ug ml A 230nm 183 A 280nm 75 8 Kalckar Shafran x mg ml A 280nm 1 45 A 260nm 0 74 Warburg Christian DNA x mg ml A 260nm 62 9 A 280nm 36 Warburg Christian Protein x mg ml A 280nm 1 55 A 260nm 0 76 Whitaker Granum x mg ml A 235nm A 280nm 2 51 Absorbance values will be determined at wavelengths as appropriate for one or more selected methods and the results are calculated and output for display Working in standard tube mode only single measurement is possible For further processing the analytical value must be saved in a separate file after each sample measurement Triggering Bio method module 1 For ScanDrop 250 Select Standard 10 mm tube
98. tical parameters are saved in the same manner as the results of measurement Consequently a result file also contains parameters for 76 06 2009 ScanDrop Quantitative Analysis Quantification Module Display amp Management of the Results of Concentration Analysis determination of concentration For this reason analytical parameters can be loaded from a parameter file or a result file 1 Press Load key in parameter window 2 Select squ file and press OK for acknowledgement Analytical parameters are displayed in the parameter window They can be edited or used in a subsequent measurement session 9 1 2 Triggering Concentration Analysis 1 Prepare analytical parameters Trigger measurement with Start Place Sample 1 reference into tube chimney following the screen prompt then press OK 4 Successively measure all other samples in the same order as defined via the sample table The samples will be automatically evaluated in accordance with your sample assignment settings 9 1 3 Display amp Management of the Results of Concentration Analysis On completion of a measurement process the results are available for viewing on the Calibration the Table and the Settings tab of the results window Its function keys are intended for saving and exporting of measured results and for returning to the parameter window Tabs in results window Calibration tab Only for concentration analysis with calib
99. tion Requirements 13 4 1 Transportation amp Storage cecccccceeeeeeeeceeeeeceeeeeaeeseeeeeseeeeeeaeeeeneeees 13 4 2 Installation REQUIreEMENKS ccceeeeeeeeeeeeceeeeeeeeeeeeeeeeteaeeeeaeeteaeeseenees 13 5 Installation amp Start Up ccccccccesssessseeeeeeeeeeeeeseeeeeeeeeees 15 5 1 Standard Delivery SCOPG ccccccceeseeeeeeeeeeeeeeeaeeeeeeeseeeeessaaeeeeneeeeaes 15 5 2 Components amp Terminals ccccccccceeceeneeeeeeeeeeeaeeeeeeeseeeeesseeesaeeesaes 16 5 3 Connecting the ScanDrop ccccecesceeeeeeeceeeeeeaeeeeeeeseeeeeseaeeseneeeeaes 19 5 4 Starting Up amp Turning ON ccceceeeeeeeeeeeee eee eeeaeeeeaeeseeeeessaeeeteneeeeaes 19 5 5 MAUI dallele Oae ceperererree error e rere rece a i a EAE 20 6 Inserting Samples ccccceeeeeeeeeeeeeeeeeeeeeeeeeeeseeenees 21 6 1 Working with a CHIPCUVETTE 0 ecccececceeeeeeeeeneeeeeeeeesaeeeeaaeeeeneeeees 21 6 2 Working with a Standard TUD c ccceeeeeeceeeeeeeeeeeeeeeseeeesnaeeneneeeees 23 7 Operation Control with ASpect Nano cccceseeeeee 25 7 1 General Settings for ASpect Nano ssssseeesesiessresrssrrssrrssrresresrns 26 7 1 4 User Identity Management amp Current User Log Off 0 eeeeeeerees 26 7 1 2 Language Settings raseria aht aoei aa aaa iinet nie 28 7 1 3 Setting Initial Screen After ScanDrop Power Becomes Available 28 7 1 4 General System Settings ccececceeeceeneeeeeeeeee
100. tration analysis performed with a CHIPCUVETTE Squ Analytical parameters and results of concentration analysis performed with a standard tube Bio method modules cbi Analytical parameters and results of a bio method performed with a CHIPCUVETTE sbi Analytical parameters and results of a bio method performed with a standard tube Expression modules cei Analytical parameters and results based on user programmed formula with a CHIPCUVETTE sei Analytical parameters and results using a programmed formula with a standard tube UV VIS modules dac Readings obtained measuring with a CHIPCUVETTE das Readings obtained measuring with a standard tube par Measurement parameters in UVVIS module 32 06 2009 ScanDrop Measurement in CHIPCUVETTE Defining Sample Assignments for CHIPCUVETTE in ASpect Nano 8 8 1 Measurement in CHIPCUVETTE Defining Sample Assignments for CHIPCUVETTE in ASpect Sample assignments for a CHIPCUVETTE must be specified before measurement can begin This will determine the order in which samples are measured in an analytical process This work step is similar in all program modules A sample assignments chart is graphically displayed For better convenience the various sample positions sample channels on CHIPCUVETTE are distributed onto the two tabs 1 8 and 9 16 A sample channel on CHIPCUVETTE is represented through a bar with its assigned number A slim bar symbolizes the shallow test point end
101. tual results of measurement and displayed when this measured data file is loaded the next time Entries at the Title input field are optional Operator User names are assigned through user identity managament Display For input of a desired unit of measure for the ordinate of measured results e Transmittance e Absorbance ScanDrop 06 2009 91 User Defined Measurement UV VIS Module Settings for amp Management of Measurement Parameters Accumulation Measuring mode tab Cycle tab e Energy Specifies the number of repetitions of a single measurement cycle you want included in calculation of a measured value This number of single readings will be averaged and output as the result of measurement in your selected unit of measure for ordinate Accumulation of a number of single readings helps improve the signal to noise ratio The Meas mode tab can be used for selection of a spectral range or of wavelengths for measurement Spectrum Fixed wavelengths Measures spectral range Specify starting wavelength and end wavelength in input fields Wavelength 1 and Wavelength 2 respectively Measures at one or more wavelengths On selection of this option a Wavelength input field and a big list field will appear e Press Wavelength input field using the superimposed numerical keypad then define a wavelength e Use 4 to hide the keypad when you have completed the last wavelength definition e Press Add
102. type in main window 2 Press Bio method key The Bio module parameter window opens 06 2009 ScanDrop Bio Method Module Selection of Bio Method amp Triggering Analysis 9 2 1 Module Bio x Sample name Methode JoNa Purty 260 280 Accumulations fio Result Corr 320 nm J Measurement values nm a Operator New Open Save Export Ref Start Default Close Fig 41 Bio module Std parameter and results window Function elements in Bio module window The Bio module window is intended for selection of a bio method and for display of analytical results Its function keys are located at the bottom bar of this dialog window Function Description New Deletes all previous entries and allows new entries for analytical parameters Open Opens a previously saved analytical file with bio methods Save Saves analytical result Export Exports the result of analysis Ref Triggers reference measurement Start Triggers measurement with presettings for analytical parameters Default Defines presettings that will be displayed in the Bio module window at the beginning of a new analytical session Close Closes the Bio module Selection of Bio Method amp Triggering Analysis A bio methiod can be selected and an analytical process be triggered in Bio module window 1 Prepare analytical parameters Make parameter settings as follows Sample name Individual
103. ut field of a desired sample line Enter a name at the alpha numerical keypad and press OK for acknowledgement A table with sample names can also be imported from a CSV file This CSV file must contain sample names in the same order as your current sample table The position of reference and standard samples must be taken into consideration Enter known analyte concentrations for standard samples Tap onto Target input field of a desired sample line Enter a desired concentration value at the numerical keypad and press OK for acknowledgement Alternatively to making entries at the sample table all inputs relating to a given sample may equally be performed in Sample setup window Press required table cell in Pos column and open sample settings window for the sample in question Besides the data described above a factor for weighed in portion Init weight and for Dilution can be specified Saving and loading analytical parameters Manipulation of analytical parameters is possible via the Load or the Save key of the parameter window The default path for file saving in Quant module is StorageCard ScanDrop Quant The file extension is squ Saving an analytical parameter file 1 2 3 Set analytical parameters you want saved in the parameter window Press Save key The standard window for file saving opens Set path and file name and confirm your selections with OK Loading of analytical parameters Analy
104. w concentration analysis session Close Closes the Quantification module ScanDrop 06 2009 73 Quantitative Analysis Quantification Module Analytical Parameter Settings 9 1 1 Analytical Parameter Settings Defining calibration parameters Measurement parameters can be set and the type and mode of concentration analysis be specified on the General tab Module QUANT x General SampleTable Title Wavelength i Multi measure 1 Unit Accumulations 10 Calibration Current sample table Regression ly A B New Save Open Load Start Default Close Fig 36 Quant module Stand calibration parameter settings Title For assigning a title to measurement This entry is optional Wavelength Sets wavelength to be used for analysis Multiple meas Defines repetition runs of a sample measurement Determines concentration from the mean value of all measured readings Unit Unit of measure applied to concentration analysis This entry appears in the results display area It will be saved together with the analytical result Accumulations The number of repetitions of a single measurement run which are involved in calculation of a measured value From the single readings a mean value is obtained which is then output as the result of measurement Absorbance Accumulation of a multiple of single measurement runs helps improve the signal to noise ratio For concentration analysis a desired type
105. y is optional Acknowledge your entries with OK and exit the Set wavelengths window Module Expression Set wave lengths x Fig 29 Unit of Results mg mL Number Ok Cancel Expression module Chip defining wavelengths for formula 4 Compile formula in Edit Expression window Available mathematical operations Designation of key Operator Constant Round brackets Addition Subtraction Multiplication Division Reciprocal value Square 3rd power Square root ScanDrop 06 2009 63 Expression Module Analytical Parameter Presettings Programming of Analytical Formula In Natural logarithm abs Amount The A keys stand for the absorbance values that were obtained at the defined wavelengths The numeral block is intended for numerical inputs By pressing Clear or Back you can delete an expression fully or delete only its last entry The Test can be used to check a formula for mathematical correctness o Enter formula for example A1 62 9 A2 36 Press OK to acknowledge this formular and return to the formula list screen Modul Expression Edit expression Al 1 45 A2 0 74 Clear Back Fig 30 Expression module Chip editing of formulas The newly edited formula appears in the list for example y mg ml A 260 62 9 A 280 36 64 06 2009 ScanDrop User Defined Measurement

ScanDrop Nano-volume Spectrophotometer

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