MVCS-MVCC manual ver4_2 - NPI Electronic Instruments
Contents
1. I If the recording chamber is not grounded connect GND of the headstage MVCS C Note System ground is isolated from mains ground The 19 cabinet EPMS H 07 housing is connected to mains ground see also chapter 2 headstage enclosures are connected to the internal system ground LI MVCS C MVCC C Connect the Ver 10 connectors and the current OUTPUT 4 Figure 5 to an oscilloscope or to a data acquisition system I If you intend to control the MVCS MVCS C system externally e g by a computer connect the gating signal to TTL 15 Figure 5 the stimulus waveform to INPUT 3 Figure 5 and the current OUTPUT 4 Figure 5 to the analog input of the data acquisition system version 4 2 page 17 MVCS MVCC User Manual 8 Operation MVCS MVCS C MVCC MVCC C systems are modules for the npi EPMS 07 system Important These modules require an EPMS 07 housing with high voltage power supply Users of systems delivered before April 2002 will recognize a built in high voltage power supply by the fact that the EPMS 07 housing is 354 mm deep instead of 243 mm with low voltage power supply Systems delivered after September 2006 have a yellow warning label above the respective channels Additionally each channel has a mark A B C D COMPENSATION and the related headstages are labeled accordingly Please use headstage A for channel A headstage B for channel B and so on because each headstage 1s dedicated to a particular2. D Electronic L Instruments for the Life Sciences ASUL e wade OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE MVCS MVCC Series IONTOPHORESIS MODULES FOR EPMS 07 SYSTEMS Vala la Wa Veta CINTIA IF NZ KI NA KEN ER E VERSION 4 2 npi 2015 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com MVCS MVCC User Manual Table of Contents ADOUCIR Mota 3 lx alety Resulta id 5 Ze EPMS 07 Modular Plu MN SS pista 6 2 1 General System Description Operation sisisi a 6 A A HOUSE eriin E E N 6 20 HOP WAST TOUS 1 ts aa 6 SIAC E EMISOR 6 2e EA 7 A PWR O De a 7 Ds AAA a 8 EPIMS O7 EBMSOS tai ti can 8 O 8 ZS Technical Dai E 8 EPMS 07 EPMS E 07 and EPMS H 07 ccccccccccccceceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeees 8 ERMS 07 and TEINS DTS E ida 8 EDMIS DT li li a d qa 8 PEMS O Senna aia 8 y MVESENVMVCO COMPONER aiii e 9 4 WY IA E O 9 Als VIVES Tontophoresis Mode ds 9 AD WIV CC 02 Balance Modulada sliciiaia 10 4d Fast Capacitance Compensa ON sesers a E E e aE a 10 dde Electrode Resistance Le aa 11 de Descnption Ol the Front Pana 12 dis Front Panel Elements A aectiaa eels tote 13 MVC C2 Cc ses oceano acidos 15 6 Headstage MVCS C MVCC C Systems ss sisvsscdeateedvinsseaszatcncacenbiatseeiatinaan viavesaeaseaunss 16 T Setting up the IONtOpnOresis S VSM ico dieta 17 8
3. Pierrefiche O Lalley P M and H R Polder 1996 Voltage clamp analysis of neurons within deep layers of the brain J Neurosci Methods 67 121 131 Iontophoretic labeling of cells I Varvel NH Grathwohl SA Baumann F Liebig C Bosch A Brawek B Thal DR Charo IF Heppner FL Aguzzi A Garaschuk O Ransohoff RM Jucker M 2012 Microglial repopulation model reveals a robust homeostatic process for replacing CNS myeloid cells Proc Natl Acad Sci U S A 109 18150 5 Fast capacity compensation Simulation of synaptic events I Behrends J C Lambert J D C and K Jensen 2002 Repetitive activation of postsynaptic GABAA receptors by rapid focal agonist application onto intact rat striatal neurones in vitro Pfliigers Arch 443 707 712 Y Cottrell J R Dube G R Egles C and G Liu 2000 Distribution Density and Clustering of Functional Glutamate Receptors Before and After Synaptogenesis in Hippocampal Neurons J Neurophysiol 84 1573 1587 I Heine M Groc L Frischknecht R Beique J C Lounis B Rumbaugh G Huganir R L Cognet L amp Choquet D 2008 Surface mobility of postsynaptic AMPARs tunes synaptic transmission Science 320 201 205 I Heine M Thoumine O Mondin M Tessier B Giannone G amp Choquet D 2008 Activity independent and subunit specific recruitment of functional AMPA receptors at neurexin neuroligin contacts Proc Natl Acad Sci U S A 105 20947 20952 I Li Y Kru
4. The circuit 1s designed to minimize oscillations Caution As in any feedback circuit this circuit can cause overshoots or oscillations if it is overcompensated 8 2 Electrode Resistance Test Procedure MVCS C MVCC C Systems MVCS C modules and MVCC C modules have an electrode resistance test unit built in It works by application of rectangular current pulses 10 nA to the electrode This will cause an voltage drop at the electrode which is proportional to the electrode resistance This voltage drop is measured the resulting electrode resistance calculated and shown at the digital display 10 Y Set switch 5 to OPERATE 1 Immerse the electrode into the bath and compensate for stray capacities see chapter 8 1 above I Set Vet leL ReL switch to Rev The resistance of the electrode is shown at display 10 Note If 5 is switched to SET the internal resistor of 10 MQ is measured and displayed If a different value is shown within a tolerance of 10 it is likely the amplifier or the headstage is damaged Please contact npi electronic in this case Important The electrode resistance is displayed correctly only if the electrode capacity is compensated accurately Note The electrode resistance test 1s functional also if an iontophoresis current is generated This feature can be used to test the resistance at different current levels For testing rectification the EJECT potentiometer or a ramp signal at the INPUT connecto
5. Oper ia 18 8 1 Capacity Compensation Tuning Procedure MVCS C MVCC C Systems 19 8 2 Electrode Resistance Test Procedure MVCS C MVCC C Systems oocccccccccnnniccnnns 19 D A O E 21 10 TechmcallD truncada llas 23 version 4 2 page 2 MVCS MVCC User Manual About this Manual Available Modules MVCS 01 one channel without headstage ReL test and CAPACITY COMPEN SATION MVCS 02 two channels without headstage Re test and CAPACITY COMPEN SATION MVCS C 01 one channel with headstage Re test and CAPACITY COMPENSATION MVCS C 02 two channels with headstage Re test and CAPACITY COMPENSATION MVCC 02 balance module for up to four channels This manual describes the MVCS 02 MVCC 02 systems The only difference between MVCS 01 and MVCS 02 systems are the number of channels Therefore the MVCS 01 systems are not mentioned separately The manual should help you setup and use the MVCS 02 MVCC 02 system correctly and to perform accurate experiments With MVCS 02 the iontophoresis is performed and MVCC 02 is the balance module for the iontophoresis system used to compensate for the iontophoretic current see also chapter 4 2 Unless otherwise noted functions and settings that apply to MVCS 02 also apply to MVCC 02 Generally two different versions of the MVCS 02 MVCC 02 system are available e System without headstage ReL test and CAPACITY COMPENSATION MVCS 02 MVCC 02 versions e System with headstage ReL te
6. for channels A and B 100 nA V Calibration for the MVCC 18 1 uA V The OUTPUT is not isolated from system ground 5 SET OPERATE switch version 4 2 page 13 MVCS MVCC User Manual PE Two position switch to set the mode of operation In SET position the electrode outputs AM are connected to an internally grounded load 10 MQ and no compensation signal is generated Thus the SET position is used to preset the desired values at the EJECT RETAIN controls on a well defined basis In the OPERATE position the current preset at the EJECT RETAIN controls will flow through the electrode and a compensation signal is generated at the MVCC 6 VeL 10 connector BNC connector monitoring the electrode potential divided by 10 Normally used to wg monitor the electrode resistance scaling 1 mV MQ see also chapters 4 4 and 8 1 7 RETAIN potentiometer 9 OVER LEDS gag LEDs indicating that the current source is out of linear range or that the electrode voltage current is 10 below the maximum output voltage current 10 CHANNEL A display a 3 1 2 digit display for the electrode potential in V XXX X V the electrode current in nA XXXX nA for channels A and B XX XX uA for MVCC or the electrode resistance in MQ XXXX MQ 1 e 0100 correspond to 100 MQ selected by toggle switch 12 For the correct value of the electrode resistance display it is necessary to adjust the capacity compensation accurately se
7. pulses of 10 nA to measure the electrode resistance These pulses are monitored at the current OUTPUT BNC and the voltage response can be seen on the Ver 10 BNC In this way changes of electrode resistance can be recorded with a chart recorder or computer based data acquisition system In addition the electrode resistance test mode can be used to tune the fast capacity compensation see chapter 8 1 Important The CAPACITY COMPENSATION unit must be tuned properly Otherwise the electrode resistance display may be inaccurate version 4 2 page 11 MVCS MVCC User Manual 5 Description of the Front Panel AWN JOVWISOVIH NILIY JOVISOWIH ANWUOO L ANUOOL INdINO _ lt ll ul 1NdNI INAINO JVUIdO O NIVIZO dNOIAVI TINNVHO TINNVHD Oldu 20 DDAW du INZO SDAMN gt TINNVHO NOUVSNIANODO g TINNVHO Y TIINNVHO Figure 5 MVCS C 02M MVCC C front panel view the numbers are related to those in the text below page 12 version 4 2 MVCS MVCC User Manual 5 1 Front Panel Elements In the following description of the front panel elements each element has a number that is related to that in Figure 5 The number is followed by the name in uppercase letters written on the front panel and the type of the element in lowercase letters Then a short description of the element is given The front panel of the MVCS C 02M can be divided into two functional units CHANNEL A and CHANNEL B T
8. same controls but no potentiometer for adjusting current In fast systems with CAPACITY COMPENSATION MVCS C MVCC C the injecting electrodes are connected via small SMB or BNC shielded connectors that are mounted to a small headstage avoiding artifacts caused by long cables Systems for slow long lasting applications in the second or minute range MVCS MVCC need no headstages In this case the electrodes are connected by special connectors at the front panel with shielded cables For EJECT or RETAIN currents modes of operation include manual activation and automatic control by digital TTL signals HI EJECT LO RETAIN An automated electrode resistance test mode MVCS C MVCC C is also available 4 2 MVCC 02 Balance Module To avoid artifacts caused by iontophoretic drug application the MVCS 02M systems can be operated with the balance module MVCC 02 The balance compensation signal inverted sum of current output signals divided by 10 generated by the MVCC module is applied to a separate compensation electrode if the OPERATE mode is selected for the injection channel s and COMPENSATE mode is selected for the compensation channel The MVCC module can compensate iontophoretic current for up to four injection channels 4 3 Fast Capacitance Compensation The MVCS C MVCC C iontophoresis instruments have been designed for high speed application of drugs in electrophysiological experiments In addition to the standard fea
9. special connectors on the front panel with shielded cables Pin 2 white blue wire ground Pin 5 yellow red wire electrode 7 Setting up the lontophoresis System The following steps should help you set up the iontophoresis system correctly Always adhere to the appropriate safety measures see chapter 1 Usually the module s are shipped mounted in an EPMS H 07 housing If a single module is delivered the user has to mount the module in the EPMS H 07 housing This is done by performing the basic installation steps Basic installation Y Turn off the EPMS 07 system I Remove front covers from the EPMS 07 housing Y Plug in the MVCS MVCC and fasten the iontophoresis balance module with four screws The screws are important not only for mechanical stability but also for proper electrical connection to the EPMS 07 housing After installation MVCS MVCS C MVCC C MVCC are attached to the setup by assembling the electrical connections Electrical connections Y Turn POWER off _I MVCS MVCC Connect your injection electrodes to the special connectors with shielded cables at the front panel of the MVCS module Connect your compensation electrode to the special connector with shielded cables at the front panel of the MVCC module Pin 2 white blue wire ground Pin 5 yellow red wire electrode MVCS C MVCC C Connect the headstages to the HEADSTAGE connectors 1 Figure 5 at the front panel of the respective module
10. view Right PWR 03D rear panel view Note This power supply is intended to be used with npi EPMS E systems only version 4 2 page 7 MVCS MVCC User Manual 2 7 System Grounding EPMS 07 EPMS 03 The 19 cabinet is grounded by the power cable through the ground pin of the mains connector protective earth In order to avoid ground loops the internal ground is isolated from the protective earth The internal ground is used on the BNC connectors or GROUND plugs of the modules that are inserted into the EPMS 07 housing The internal ground and mains ground protective earth can be connected by a wire using the ground plugs on the rear panel of the instrument It is not possible to predict whether measurements will be less or more noisy with the internal ground and mains ground connected We recommend that you try both arrangements to determine the best configuration EPMS E 07 The 19 cabinet is connected to the CHASSIS connector at the rear panel It can be connected to the SYSTEM GROUND SIGNAL GROUND on the rear panel of the instrument see Figure 4 The chassis can be linked to PROTECTIVE EARTH by connecting it to the PWR 03D with the supplied 6 pole cable and by interconnecting the GROUND and PROTECTIVE EARTH connectors on the rear panel of the PWR 03D see Figure 3 Best performance is generally achieved without spy NES y 1 e connection of the chassis to protective earth SIGNAL HASS GROUND e IS ES Imp
11. 4 to AUTO and applying a TTL pulse to 15 version 4 2 page 18 MVCS MVCC User Manual 8 1 Capacity Compensation Tuning Procedure MVCS C MVCC C Systems The tuning of the capacity compensation controls is performed with the help of the electrode potential monitor BNC marked Ver 10 6 and square pulses applied to the electrode This pulse can originate from the built in ELECTRODE RESISTANCE TEST circuit or from an external signal source The pulses generated internally by the ELECTRODE RESISTANCE test unit have an amplitude of 10 nA The following tuning procedure is described for the MVCS C channels A or B only The tuning of the capacity compensation for the MVCC C module is done analogue The tuning must be performed with the electrode in the bath immersed to the maximal depth required during the experiment Square pulses positive and negative of a few nA and 0 1 10 ms duration are applied to one of the INPUT BNCs 3 or by activating the ELECTRODE RESISTANCE test unit 13 The signals from the Ver 10 and CURRENT OUTPUT BNCs 4 are monitored on an oscilloscope The SET OPERATE switch 5 must be in OPERATE position The CAP COMP control 8 is turned on clockwise until the signal at the Ver 10 BNC is as square as possible The CAPACITY COMPENSATION is based on the well known conventional compensation stray capacitances around the electrode are compensated by passing amounts of the electrode signal through a small capacitor
12. channel Each system is composed of one MVCS 01 MVCS C 01 or two independent injection channels MVCS 02 MVCS C 02 marked A and B MVCC MVCC C systems serve as compensation balance channels and can compensate iontophoretic current for up to four injection channels Each channel has an auxiliary analog input and an output which monitors the current flowing through the electrode Each channel is equipped with a digital display and two overload LEDs All numbered items refer to Figure 5 page 12 in the following discussion The systems can be operated manually by means of a toggle switch on the front panel 14 or by an external digital pulse TTL connected to 15 Turn CAP COMP 8 for all channels to less than 1 to avoid oscillations Turn POWER on Set the operation mode of all channels to SET using switch 5 to disable current output Set the EJECT and or RETAIN current amplitude to the desired values using 11 and 7 DOCCOL L MVCS C First compensate the stray capacitances of the electrodes and second check the electrode resistances by switching 13 to Rex see also chapter 8 1 below Important The values of the ELECTRODE RESISTANCE are accurate only if the capacitances of the electrodes are compensated properly 1 Put the injection and compensation electrodes to the desired positions J Start iontophoresis either manually by setting switch 14 to EJECT and switch 5 to OPERATE or remotely by setting switch 1
13. e chapter 8 1 11 V nA MQ LEDs LEDs indicating the unit of the reading of the DISPLAY 10 version 4 2 page 14 MVCS MVCC User Manual 12 EJECT potentiometer Ten turn control to set the EJECT current range 0 1 uA 13 Vet leL ReL switch NW 3 position toggle switch to set the mode of display CHANNEL A 10 d Position Ver the electrode potential is displayed Position Ier the current flowing through the electrode is displayed Position Ret the electrode resistance is displayed Important The Ret mode is an option that is only implemented in MVCS C MVCC C fast systems In MVCS MVCC slow systems the Ret position of the switch has the same function as the Ig position middle position 14 EJECT RETAIN AUTO switch Switch to select the mode of operation EJECT the EJECT current set with 11 is applied to the electrode RETAIN the RETAIN current set with 7 is applied to the electrode AUTO Operation controlled by a TTL pulse at 15 Remember Current is applied to the electrode only if switch 5 is set to OPERATE 15 TTL connector O Y BNC connector for external control in the AUTO mode see also 14 LO RETAIN me HI EJECT MVCC C Only 16 MODE switch 17 INPUT connector 18 OUTPUT 1u A V connector For 17 and 18 see above 16 MODE switch Switch to select the operation mode of the MVCC C COMP The inverted sum of all injection channels is appli
14. ed to the electrode OFF No current 1s applied to the electrode EXTERN The output current source is connected directly to INPUT BNC 17 In this mode the MVCC can be used as an additional injection channel with a scaling of 1 uA V 1 e if 1 V is connected at 17 an injection current of 1 uA is applied to the electrode connected to the MVCC version 4 2 page 15 MVCS MVCC User Manual 6 Headstage MVCS C MVCC C Systems The headstage is housed in a small box that can be mounted directly onto a micromanipulator It is connected to the main amplifier by means of a shielded flexible cable and a multi pole connector electrode holder headstage optional D 71732 Tamm www npielectronic com y Pm IONTOPHORESIS HEADSTAGE e bie men A B C D COMP OPERATE t voLTAGEI Y O O O U o cm CHANNEL 2 3 Figure 6 MVCS C 02C headstage 1 Pet BNC connector for the electrode holder grounded shield 2 CHANNEL indicator marker show for which CHANNEL the headstage is configured in the example shown for CHANNEL A see also hint below 3 holding bar and headstage cable to amplifier 4 GND ground connector 5 OPERATE LED indicates that injection takes place GND GROUND connector The bath or reference of the recording chamber is connected to GND This is the lowest signal level in the recording system i e all signals are related to this signal This connector must be connected to the ground signal o
15. f the recording amplifier chamber PEL In order to avoid disturbances on the recording amplifier the microelectrode holder is connected via a BNC connector with a grounded shield Caution The current injection headstages have an output compliance of 45 V up to 150 V In addition all headstages are equipped with sensitive FET amplifiers that can be damaged by electrostatic charge and must therefore be handled with care see also chapter 1 Very Important Always turn power off when connecting or disconnecting headstages from the 19 cabinet For changing electrodes it is sufficient to switch the respective channel to SET mode Also very important Each headstage is adjusted for a specific channel and instrument They are labeled A and B or A B C D for a four eject channel system formed by two MVCS C 02M modules for the EJECT channels and C for the COMPENSATION channel Please do not exchange headstages for a respective instrument or between different MVCS instruments Hint If one channel is not used the headstage can be left unconnected but then the display for this channel will show arbitrary values So we recommend to connect the headstage even if it is not used and set channel to SET mode using switch 5 Figure 5 version 4 2 page 16 MVCS MVCC User Manual Systems for slow long lasting applications in the second or minute range need no headstages MVCS systems In these systems the electrodes are connected from
16. he MVCC C module serves for balancing the iontophoretic current for up to four injection channels Most of the elements are identical for each channel with identical functions and labels and therefore are numbered and described only once e g 1 HEADSTAGE connector that is also present for CHANNEL B and the MVCC Figure 5 shows the MCVS C 02 MVCC C fast systems with capacity compensation and the automatic electrode resistance test facility These two features are not present in the MVCS 02 MVCC slow systems In slow systems the CAP COMP potentiometer is not installed and the function of Re is somewhat different see below 1 HEADSTAGE connector 8 pole connector for the HEADSTAGE MVCS C systems or for the cable directly connected to the injecting electrode MVCS systems 2 NORMAL INVERTED switch NOR a Switch to set polarity of EJECT and RETAIN current NORMAL EJECT positive meq RETAIN negative 3 17 INPUT connectors BNC connectors for an auxiliary INPUT These BNCs are directly connected to rA RS the output current source and are not isolated from ground Calibration for channels A and B 100 nA V Calibration for the MVCC 17 1 uA V Note The MVCC can be used as an additional injection channel by linking an external waveform to this connector and setting switch 16 to EXTERN 4 18 OUTPUT connectors G BNC connector monitoring the EJECT or RETAIN current ouput s Calibration
17. idelberg New York highly recommended I Ogden D ed 1994 Microelectrode Techniques The Company of Biologists LTD Cambridge J Purves R D 1981 Microelectrode Methods for Intracellular Recording and lontophoresis London Academic Press I Roberts M H T and T Gould 1993 Iontophoresis in the mammalian central nervous system in D I Wallis ed Electrophysiology A Practical Approach Oxford University Press Oxford _I Zieglg nsberger W 1992 Arrays for Microiontophoresis in Kettenmann H amp Grantyn R eds Practical Electrophysiological Methods Wiley Liss New York version 4 2 page 22 MVCS MVCC User Manual 10 Technical Data Electrode output Maximum current Display Over LEDs Eject Minimum pulse duration Retain Capacity compensation Output current polarity Modes of operation floating current source output impedance gt 10 Q 450 nA 1 5 uA into 100 MQ load current XX XX nA compensation XX XX uA voltage XXX X V Re XXXX MQ displayed value is set by a three position toggle switch separate displays for each channel activated 10 below maximum current voltage adjustable by ten turn control 50 us adjustable by ten turn control maximum 100 nA adjustable by ten turn control range 0 30 pF selected by INVERTED NORMAL toggle switch set by two toggle switches EJECT RETAIN AUTO switch enables manual or TTL controlled operation SET OPERATE switch connects automatica
18. ing a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME All analog electronic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition 1 e after internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient HANDLING Please protect the device from moisture heat radiation and corrosive chemicals CURRENT INJECTION HIGH VOLTAGE HEADSTAGE The current injection headstages have an output compliance of 45 V up to 150 V In addition some headstages are equipped with a driven shield electrode connector marked Driven Shield on the headstage enclosure After turning on the instrument do not touch the interior contact or the shield of the electrode plug or of the cable that is connected to this plug In addition it is extremely important that the instrument is turned off when changing or adjusting the electrode version 4 2 page 5 MVCS MVCC User Manual 2 EPMS 07 Modular Plug In System 2 1 General System Description Operation The npi EPMS 07 is a modular system for processing of bioelectrical signals in electrophysiology The system is housed in a 19 rack mount cabinet 3U ha
19. k with up to 150 V generating currents up to 1 5 uA into 100 MQ Generally two different versions of the MVCS 02M system are available e System without headstage ReL test and CAPACITY COMPENSATION MVCS 02M versions e Systems with headstage ReL test and CAPACITY COMPENSATION MVCS C 02M versions Note In this manual the slow MVCS 02M version is referred as MVCS whereas the fast MVCS C 02M version is referred as MVCS C The operating and display elements of these instruments facilitate the application of drugs in physiological pharmacological and biochemical studies All systems allow very fast drug applications in the millisecond range and even the sub millisecond range if equipped with the fast capacitance compensation option Therefore these systems can be used to simulate synaptic events Behrends et al 2002 Renger et al 2001 Cottrell et al 2000 Liu et al 1999 The MVCS systems are available as 19 instruments or as modules for the EPMS 07 modular system The systems described here are EPMS 07 modules The MVCS 01M has one channel for current injection while the MVCS 02M consists of two independent injection channels Each injection channel has digital ten turn potentiometer for EJECT or RETAINING currents and CAPACITY COMPENSATION Each injection channel also has a digital display over version 4 2 page 9 MVCS MVCC User Manual range LEDs and two switches for selection of the operating mode The MVCC has the
20. lly electrode outputs via a 10 MQ resistor to ground SET position TTL input AUTO mode LO RETAIN HI EJECT isolated Analog input Current monitor Voltage monitor Electrode resistance test sensitivity 100 nA V Rin gt 100 KQ range 10 V sensitivity 100 nA V Rout 50 Q VeL 10 Rout 50 Q 1 mV MQ at voltage monitor VeL 10 MVCC output inverted sum of all injection currents up to four channels sensitivity LuA V Output connector pins and cable colors Pin 2 white blue wire ground for systems without headstage Pin 5 yellow red wire electrode Dimensions MVCS 01 Front panel 18 HP 91 1 mm x 3U 128 5 mm Housing 7 175 mm deep MVCS 02 Front panel 36 HP 182 5 mm x 3U 128 5 mm Housing 7 175 mm deep MVCC 02 Front panel 12 HP 60 75 mm x 3U 128 5 mm Headstage size version 4 2 Housing 7 175 mm deep approx 70x25x25 mm page 23
21. ortant Always adhere to the appropriate safety measures Figure 4 Rear panel connectors of the EPMS E 07 2 8 Technical Data EPMS 07 EPMS E 07 and EPMS H 07 19 rackmount cabinet for up to 7 plug in units Dimensions 3U high 1U 1 3 4 44 45 mm 254 mm deep EPMS 07 and EPMS H 07 Power supply 115 230 V AC 60 50 Hz fuse 2 A 1 A slow 45 60 W EPMS E 07 External power supply PWR 03D 115 230 V AC 60 50 Hz fuse 1 6 0 8 A slow Dimensions of external power supply W x Dx H 225 mm x 210 mm x 85 mm EPMS 03 Power supply 115 230 Volts AC 60 50 Hz fuse 0 4 A 0 2 A slow Maximum current supply 500 mA version 4 2 page 8 MVCS MVCC User Manual 3 MVCS MVCC Components The following items are shipped with a MVCS MVCC system MVCS MVCC amplifier module Headstage fast MVCS C MVCC C systems only GND connector for headstage 2 6 mm fast MVCS C MVCC C systems only Electrode cables slow MVCS MVCC systems only User manual SNA NN Optional accessories Electrode holder Electrode adapter with BNC and SMB connector 4 System Description 4 1 MVCS lontophoresis Module MVCS 02M systems are high voltage current sources for iontophoresis or other applications where constant currents in the nano or microampere range are needed Standard MVCS 02M systems have an output compliance of 45 V and can generate currents up to 450 nA into 100 MQ while high voltage MVCS 02M systems wor
22. pa B Kang J S Bolshakov V Y amp Liu G 2009 Glycine Site of NMDA Receptor Serves as a Spatiotemporal Detector of Synaptic Activity Patterns J Neurophysiol 102 578 589 I Liu G Choi S and R W Tsien 1999 Variability of Neurotransmitter Concentration and Nonsaturation of Postsynaptic AMPA Receptors at Synapses in Hippocampal Cultures and Slices Neuron 22 395 409 I Murnick J G Dub G Krupa B and Liu G 2002 High resolution iontophoresis for single synapse stimulation J Neurosci Meth 116 65 75 I M ller C Beck H Coulter D Remy S 2012 Inhibitory control of linear and supralinear dendritic excitation in CA1 pyramidal neurons Neuron 75 85 1 64 I Renger J J Egles C and G Liu 2001 A Developmental Switch in Neurotransmitter Flux Enhances Synaptic Efficacy by Affecting AMPA Receptor Activation Neuron 29 469 484 I Slutsky I Sadeghpour S Li B amp Liu G 2004 Enhancement of synaptic plasticity through chronically reduced Ca2 flux during uncorrelated activity Neuron 44 835 849 I Tang Y P E Shimizu G R Dube C Rampon G A Kerchner M Zhuo G Liu and J Z Tsien 1999 Genetic enhancement of learning and memory in mice Nature 401 63 69 version 4 2 page 21 MVCS MVCC User Manual General methods I Lalley P M 1999 Microiontophoresis and Pressure Ejection in U Windhorst and H Johansson eds Modern Techniques in Neuroscience Research Springer Berlin He
23. ply to minimize distortions of the signals caused by the power supply version 4 2 page 6 MVCS MVCC User Manual 2 5 EPMS 03 The following items are shipped with the EPMS 03 housing Y EPMS 03 cabinet with built in power supply Y Mains cord Y Fuse 034 A 0 2 A slow inserted Y Front covers DE DES aa NO 8 9 A IN A OLA OU Figure 2 Left front view of EPMS 03 housing Right rear panel detail of EPMS 03 and EPMS 07 housing In order to avoid induction of electromagnetic noise the power supply unit the power switch and the fuse are located at the rear of the housing see Figure 2 right 2 6 PWR 03D The external power supply PWR 03D is capable of driving up to 3 EPMS E housings Each housing is connected by a 6 pole cable from one of three connectors on the front panel of the PWR 03D to the rear panel of the respective EPMS E housing see Figure 3 Figure 4 A POWER LED indicates that the PWR 03D is powered on see Figure 3 left Power switch voltage selector and fuse are located at the rear panel see Figure 3 right Note The chassis of the PWR 03D is connected to protective earth and it provides protective earth to the EPMS E housing if connected i npl l UPETE IE ee eesti LO BING He ARA MNAC O BONE y j nt N j hgt oioctonio GMBH Pa C 1171432 Tarn Gerrea Wwe ale ironic Oc ri Gai OUTPUT A 0 OFF Figure 3 Left PWR 03D front panel
24. r can be used for setting a current level version 4 2 page 19 MVCS MVCC User Manual Testing of rectification of the electrode is done e g by application of different current levels both positive and negative to the electrode using the EJECT potentiometer and testing the resistance of the electrode If the resistance changes at a certain level the electrode rectifies and should not be used for application of currents above or below this level 1 e the resistance must not change over the range of current which is used during the experiment Also Important Testing the electrode resistance at different current levels will cause the substance in the electrode to leak out of the electrode Therefore don t test the electrode resistance intensively within your preparation in order to avoid 1 e desensitization undercompensated potential mV 250 gt 200 4 150 100 50 4 0 T T T T 1 0 10 20 30 40 50 time ms overcompensated potential mV 600 400 200 0 4 200 400 600 0 10 20 30 40 50 time ms compensated potential mV 250 200 150 100 4 50 4 0 T T T T z T 1 0 10 20 30 40 50 time ms potential Figure 7 Capacity compensation of the electrode version 4 2 page 20 MVCS MVCC User Manual 9 Literature Iontophoresis and drug application during single electrode voltage clamp experiments I Richter D W
25. ronic disclaims any warranties for such purpose Equipment supplied by npi electronic must be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 6 GENERAL This system is designed for use in scientific laboratories and must be operated by trained staff only General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and must therefore be handled with care Electrostatic discharge can be avoided by touch
26. s room for up to 7 plug in units The plug in units are connected to power by a bus at the rear panel The plug in units must be kept in position by four screws M 2 5 x 10 The screws are important not only for mechanical stability but also for proper electrical connection to the system housing Free area must be protected with covers 2 2 EPMS 07 Housing The following items are shipped with the EPMS 07 housing Y EPMS 07 cabinet with built in power supply Y Mains cord Y Fuse 2 A 1 A slow inserted Y Front covers Figure 1 Left front view of empty EPMS 07 housing In order to avoid induction of electromagnetic noise the power supply unit the power switch and the fuse are located at the rear of the housing see Figure 2 right 2 3 EPMS H 07 Housing In addition to the standard power supply of the EPMS 07 the EPMS H 07 has a built in high voltage power supply This 1s necessary for all MVCS MVCC modules the HVA 100 HV TR 150 and HVC 03M modules The output voltage depends on the modules in use 2 4 EPMS E 07 Housing The following items are shipped with the EPMS E 07 housing EPMS E 07 cabinet External Power supply PWR 03D Power cord PWR 03D to EPMS E 07 Mains chord Fuse 1 6 A 0 8 A slow inserted Front covers SNNNNN N The EPMS E 07 housing is designed for low noise operation especially for extracellular and multi channel amplifiers with plugged in filters It operates with an external power sup
27. st and CAPACITY COMPENSATION MVCS C 02 MVCC C 02 versions Note In this manual the slow MVCS 02 MVCC 02 version 1s referred as MVCS MVCC whereas the fast MVCS C 02 MVCC C 02 version is referred as MVCS C MVCC C If you are not familiar with the use of instruments for iontophoretic application of substances please read the manual completely The experienced user should read at least chapters 1 5 and 6 Important Please read chapter 1 carefully It contains general information about safety regulations and how to handle highly sensitive electronic instruments version 4 2 page 3 MVCS MVCC User Manual Signs and conventions In this manual names of all elements of the front panel are written in capital letters as they appear on the front panel System components that are shipped in the standard configuration are marked with Y optional components with In some chapters the user is guided step by step through a certain procedure These steps are marked with Ld Important information hints and special precautions are highlighted in gray Abbreviations ler current at electrode Re electrode resistance VeL voltage at electrode version 4 2 page 4 MVCS MVCC User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical purposes e g for diagnosis or treatment of humans or for any other life supporting system npi elect
28. tures of the slow MVCS MVCC devices each channel has a capacity compensation circuit and an Rex test unit see chapter 4 4 The capacity compensation circuit is operated by the control marked CAP COMP The correct tuning of the capacity compensation is very important if high speed operation with high resistance microelectrodes is required Uncompensated stray capacitances are charged from the iontophoretic current that is supplied by the instrument Uncompensated stray capacitance therefore slows application The tuning procedure is described in chapter 8 1 The CAPACITY COMPENSATION control is based on the well known conventional compensation stray capacitances around the electrode are compensated by passing amounts of the electrode signal through a small capacitor The circuit is set so that overshoots are avoided as far as possible Caution Just like any feedback circuit this circuit can cause overshoots or oscillations if it is overcompensated version 4 2 page 10 MVCS MVCC User Manual 4 4 Electrode Resistance Test MVCS C MVCC C systems are equipped with an automatic electrode resistance test facility By switching the VeL IeL ReL switch to Ret the value of the electrode resistance is shown on the digital display in MQ whether current is applied or not This means that changes in electrode resistance caused by high currents rectification effects can be monitored directly The electrode resistance test uses current
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