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Smartline UV/VIS Detector 2550 user manual

1. Fig 3 Rear view Detector 2550 User Manual V5193A Version 1 0 19 Startup Note Prerequisite KNAUER Checklist before initial operation Use this checklist to determine whether the detector is ready for initial startup Devices are in the correct location Note Observe the ambient conditions and space require ments The power connection of the detector is plugged in If the detector is part of a HPLC system the following must be observed and operated with chromatography software The network connection to the router is established Chromatography software either from KNAUER or another software supplier has been installed The capillaries in the solvent bottles have a filter insert Capillaries from the column to the detector and capillaries from the detector to the waste bottle are securely attached Before the detector is ready to use for measurements a flow cell must be installed You can change the optical path length of a preparative flow cell before assembling the flow cell The opti mal path length depends on type and quantity of the sample Installation of the flow cell The supply configuration of this detector only includes a test cell dummy cell from KNAUER which does not have connections for solvent It is used for example to check the lamp intensity as a dirty flow cell can distort this value Before using the device with solvents a KNAUER flow cell must be installed
2. Push the depressor tool into the opening on the spring strip Continue pushing the mo depressor tool down and lead the cable into the front end of the spring strip wi Remove the depressor eee tool Check whether the cables Fig 6 Spring strip are tightly attached Plug the spring strip onto the pin header Nextsteps Finish the installation and perform the initial startup Terminal strip Event and remote control on the rear of the device The electric terminal strip Event and remote control is used to exchange signals with other devices Terminal strip Event and remote control KNAUER Detector 2550 User Manual V5193A Version 1 0 24 Connecting the detector to other devices Relay contact Relay dropped outO Relay actuated1 Pulse relay actuated for 1000 ms Permissible load of the relay contact 1 A 30V EV 2 TTL output Possible levels Low 500 mV O High 22 V 1 Pulse 22 V for 1000 ms To ensure secure operation the load resis tance of the output may not drop below 10 kQ ERROR IN TTL input When an error signal is received running programs are terminated and the mes sage Error input activated appears START IN TTL input When a signal is received a program is started in HOLD mode or a link with the corresponding WAIT parameter is started AUTOZERO The current measurement signal is set to zero Provides a voltage of 5 V with respect to GND This
3. A list of the flow cells that can be used can be found in the chapter Available flow cells for the Smartline detector 2550 Detector has been switched off Power plug has been pulled Detector 2550 User Manual V5193A Version 1 0 20 Startup Legend Q Inlet of the flow cell Path length of the flow cell 10 mm G Light path Outlet of the flow cell Knurled head screws Cover plate Carriage Fig 4 installing the flow cell Installing the flow cell 1 Open the device door and loosen and remove both knurled head screws by hand 2 Hold the flow cell securely and pull the carriage out towards the front 3 Lift up and remove the test cell located in the carriage with two fingers 4 Insert the new flow cell in the carriage and ensure that the engraved path length of the cell points towards you and the alignment pin of the detector housing fits into the corre sponding hole on the rear of the cell 5 Push the carriage onto the housing Insert both screws and tighten manually 6 Connect the incoming and outgoing capillaries at the inlet and outlet of the flow cell Changing the path length of a preparative flow cell At delivery the path length of a preparative flow cell is set to 2 mm at the factory To reduce it to 1 25 or 0 5 proceed as fol lows This description applies for the preparative flow cells with order numbers A4066 A4067 and A4068 with 1 8 or 1
4. 10 Safety More safety relevant information is listed in alphabetical order in the following table Eluent lines Install capillaries and hoses in such a way that liquids cannot get into the interior in case of a leak Flammability Organic eluents are highly flammable Since capillaries can detach from their screw fittings and allow eluent to escape it is prohibited to have any open flames near the analytical sys tem Regularly check if any system components are leaking Power cable Defective power cables are not to be used to connect the device and the mains power Power strip If several devices are connected to one power strip always consider the maximum power consumption of each device Power supply Only connect devices to voltage sources whose voltage equals the device s voltage Self ignition point Only use eluents that have a self ignition point higher than 150 C under normal ambient conditions Solvent tray Risk of electrical shock or short circuit if liquids get into the device s interior For this reason place all bottles in a solvent tray Toxicity Organic solvents are toxic above a certain concentration Ensure that work areas are always well ventilated Wear pro tective gloves and safety glasses when working on the device Where is use of the Never use the system in potentially explosive atmospheres with device prohibited out appropriate protective equipment For further information contact the Technic
5. Detector 2550 User Manual V5193A th KNALIER Table of Contents Note For your own safety read the manual and always observe the warnings and safety information on the device and in the user manual Intended Use oos a eo Dr Ro Etc R oa ueque ea 6 Local network and automatic configuration lle 6 Operating tange ww sees Gale daa Ge Taner UR DL Sg OP ATI ADR ER 6 Where is use of the device prohibited 0 eee eee eee 7 Features viuo sede edt vu rea quedo d qug CV E eaa ea qua te pet deg d 7 El rilS i Sa serene Tera Iste opea ea ed eur Dear ird ater dove p hes 8 Safety cod vat degunt Od FER DUE eee EE E SOLE ERE 9 Definition of Personal and Material Damage ssllleeees 11 Decontamination uxo oa e ae MeL RI E rm sete tics RCRUM rte nets wae 11 Symbols and Signs suci A ao sees eae EECPEEGIUEUPMEST T ERERS 12 Unpacking and Setup ees cp I REN ARG OY EP EE I ERA EE 13 Contacting the Technical Support 2 eee eee eee eee eee 13 Location Requirements 4 boca bee d Sede te oo E Ka e Cre EG 13 Ventilation 7er aep doe Go al et aw ail Cedar quise dicetis Caesia 13 Space Regulremernts S arsena ene date PEN eae So RU CV oe toe e a 13 Power Supply and Connection sd eue e x ees ert CR t eR RR 14 Power Cable 3 555 ses t eee ad un quoe uv dons end une Greta aepo ire 14 POWGPPIUQ i re Se YER FE EE USE s Se ayaa 14 Packaging and transport 2 23 res eX eR SAX RETE Rhe 14 Remove fastenin
6. Prog offO on 1 au au Prog Link Hold 0 Time A1 A2 Run 1 min nm nm Load Events 1 2 Signal 1 Signal 2 Prog off 0 on 1 au au Prog P20 Time 12 09 WAKE UP Setup Link4 Date 12 11 09 P GLP Hold 0 Time A1 A2 Run 1 min nm nm Load Events 2 Signal 1 Signal 2 Prog offO on 1 au au Prog P20 Time 15 00 WAKE UP Setup Link Date 12 11 09 P01 jJ GLP Hold 0 Time A1 A2 Run 1 min nm nm Fig 16 Creating a wakeup program Detector 2550 User Manual V5193A Version 1 0 48 Navigating through the Link menu Increase or decrease number values Procedure KNAUER Controlling the detector Controlling the Link menu Links are references between existing programs which can be defined and edited like the programs themselves Links are created and listed in the Link menu Use the horizontal arrow keys to navigate through the two lines of the display A diamond icon is displayed on the left of the lower line Move the cursor onto the diamond icon to navigate through the subitems of the Link menu by means of the vertical arrow keys With the vertical arrow keys you can increase or decrease the number values by one value T Use the horizontal arrow keys to move the cursor to the desired position in the display 2 Use the number keys to enter a desired value Move the cursor onto the diamond icon to navigate through the further subitems of the Link menu by
7. p Setup Link lt p GLP Hold 0 Time M 2 Run 1 min nm nm Load Events 1 2 Signal 1 Signal 2 Prog off 0 on 1 au au Prog Delete programm P gt Setup Link GLP Hold 0 Time A1 2 Run 1 min nm nm Load Events 2 Signal 1 Signal 2 Pro offO on 1 au au Prog Delete programm P01 Setup Link confirm with up down key GLP Hold 0 Time A1 A2 Run 1 min nm nm Fig 15 Deleting a program KNAUER Detector 2550 User Manual V5193A Version 1 0 47 Procedure KNAUER Controlling the detector Creating a program with preselectable start time Program number 20 serves as a wake up program Here a pro gram or link can be loaded at a prescribed time The device is switched on from standby mode at the desired time the D sources is ignited and put into the so called HOLD state Note Make sure that the date and time configured in the Setup menu are correct 1 Select the Program menu 2 Enter program number 20 3 Press horizontal arrow key to go to the editing mode of the submenu 4 Enter the date and time for the program start 5 Enter the number of the program that should start at the pre set time To interrupt the current WAKE UP program enter another pro gram number Load Events 2 Signal 1 Signal 2 Prog off on 1 au au Prog 4 Link Hold 0 Time A1 A2 Run 1 min nm nm Load Events 1 2 Signal 1 Signal 2
8. C 39 2 77 0 F Isopropanol Carbon dioxide liquid 99 999 CO Methanol Phosphate buffer solu tions 0 5 M Toluol Dilute ammonia solu tion Dilute acetic acid 10 50 at 25 C 77 0 F Dilute sodium hydrox ide 1M Water Intended Use Eluents Even small quantities of other substances such as additives modifiers or salts can influence the durability of the materials The list of selected solvents was compiled based on research in the pertinent literature and is only a recommendation If there is any doubt contact the Technical Support of the manufacturer Less suitable eluents Not suitable eluents Dimethyl sulfoxide DMSO Slightly volatile eluents Methylene chloride Tetrahydrofuran THF Dilute phosphoric acid valid for the specified temperature range KNAUER Halogenated hydrocar bons e g Freon Concentrated mineral and organic acids Concentrated bases Eluents containing parti cles Perfluorinated eluents e g Fluorinert FC 75 FC 40 Perfluorinated polyether e g Fomblin Detector 2550 User Manual V5193A Version 1 0 9 Safety Safety Professional group The user manual is addressed to persons that are qualified as chemical laboratory technicians or have completed comparable vocational training The following knowledge is required Fundamental knowledge of liquid chromatography Knowledge regarding substances that are suitable only to a limited
9. Inform the Technical Support of the manufacturer in case the system message repeats itself The system messages are sorted alphabetically 5 phase motor Switch the device off and on again Check init failed cable connection If the system message appears again notify KNAUER Technical Support At least one Check whether at least one channel is on wavelength must Check that the entered the wavelengths be valid are in the permissible range 190 nm to 900 nm Calibration failed Restart the device Check that lamps motor and filter are functioning correctly If the system message appears again notify KNAUER Technical Support Restart calibration at the device or in the chromatography software Cannot delete First pause link then delete program active program link Cannot edit First pause link then edit data using program from chromatography software the running link Cannot initialize Check cables and connections in local area LAN network Cannot operate Restart the device Wait until calibration is an uncalibrated completed If the system message appears instrument again restart the device Cannot operate Create a link with an empty link Cannot proceed Wait until D2 lamp has preheated D2 lamp heating Cannot proceed Test whether the lamps have been lamps are off switched on Cannotread data Restart the device from FRAM If the system message appears again notify KNAUER Technical Sup
10. quality assurance for laboratories Gradient Time dependent composition of solvent mobile phase on low pressure or high pres sure side of system High performance liquid chromatography Integration The integration time determines how fast time the detector reacts to changes in absor bance IP address Unique address of transmitter or receiver in local network or Internet Internet protocol Solvent Mobile phase eluent or carrier for liquid chromatography Remote The detector is completely controlled by the chromatography software Dead volume Volume of thin flexible stainless steel capil laries between mixing chamber injector and column as well as between column and detector The dead volume should as small as possible UHPLC Liquid chromatography in the ultra high pressure or ultra high performance range KNAUER Detector 2550 User Manual V5193A Version 1 0 lh KNALIER Declaration of Conformity Manufacturer name and address Detector Smartline 2550 Europe Wissenschaftliche Ger tebau Dr Ing Herbert Knauer GmbH Hegauer Weg 38 14163 Berlin Germany Product number E4311XA E4312XA complies with the following requirements and product specifica tions DIRECTIVE 2006 42 EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 17 May 2006 on machinery and amending Directive 95 16 EC recast IEC 60799 June 1998 Electrical accessories Cord sets and interconnection cord sets IEC 61010 1
11. 2010 Corrigendum 2011 Safety require ments for electrical equipment for measurement control and laboratory use IEC 61010 2 081 2001 A1 2003 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 081 Particular requirements for automatic and semi automatic laboratory equipment for analysis and other purposes IEC 61010 2 010 2003 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 010 Particular requirements for laboratory equip ment for the heating of materials Low voltage directive 2006 95 EC IEC 61000 3 2 2005 A1 2008 A2 2009 Electromagnetic compatibility EMC Part 3 2 EMC standard 2004 108 EC IEC 61326 1 2012 Electrical equipment for measurement control and laboratory use EMC requirements Part 1 Gen eral requirements Directives for an environmentally sound use of electrical and electronic equipment RoHS directives 2002 95 EC 2003 and 2011 65 EU on the restriction of the use of certain hazardous substances in electrical and electronic equipment WEEE directive 2002 96 EC 2003 and 2012 19 EU on waste electrical and electronic equipment The device was tested with a standard configuration Berlin 2013 10 23 AT Dr Alexander B nz Managing Director The mark of conformity has been applied to the rear panel of the device Ce Table O Fig Fig Fig Fig Fig Fig Fig Fig Fig
12. Holmium After device calibration the spectral refer ence values are measured with an internal GLP Holmium holmium filter as well as the H beta line of the deuterium lamp as nor GLP H beta mal line and their position verified If the difference between nominal and actual value is less than specified in the device s data sheet the line position is marked o k GLP optical BW Display of the results of the self test for stray light optical bandwidth and approximate lin earity The linearity is determined by a short procedure for determination of scattered light at a spectral point during calibration GLP D2 Display of the serial numbers of the installed lamps their operating time and GLP Hal in case of the deuterium lamp also the number of lamp starts If a source is not installed it is indicated by KNAUER Detector 2550 User Manual V5193A Version 1 0 43 Procedure KNAUER Controlling the detector GLP time Display of the total operating time of the detector and the run time of the grating motor The grating motor operating times are only registered when the device operates in multiple channel mode Controlling the Program menu The Program menu is used to list and create programs The 2550 detector can save up to 19 programs and up to 99 program lines Program 20 is reserved for a special wake up program for a time delayed execution of the links or programs Max 10 program links between defined program
13. KNAUER Detector 2550 User Manual V5193A Version 1 0 57 Legend Q Inlet of the flow cell Outlet of the flow cell G Knurled head screws Cover plate Carriage Prerequisite Procedure Result KNAUER Maintenance and care Replacing the flow cell UV light will cause the flow cells to become blind with time solarization making them no longer suitable for use A list of the flow cells that can be used can be found in the chap ter Available flow cells for the Smartline detector 2550 Fig 22 Replacing the flow cell The device is switched off and the power cable has been unplugged The following steps are recommended for disassembling the flow cell 1 2 3 Switch off the device and pull the power plug Open the device door Remove capillaries at the inlet 1 and outlet 2 of the flow cell Unscrew the knurled head screws 3 of the cover plate Hold the flow cell securely with your hand during this proce dure Pull out the carriage of the flow cell 5 towards the front 6 Lift the flow cell up and out Insert new flow cell from above into the carriage Continue to hold the flow cell securely Push the carriage back into the detector Screw the knurled head screws back into the cover plate 4 and tighten Flow cell has been replaced Detector 2550 User Manual V5193A Version 1 0 Disposal 58 AVV marking in Ger many WEEE registration El
14. USB to COM box the option Allow the computer to turn off this device to save power in the Device Manager must be deactivated for all USB hosts Only for Windows 7 For the network adapter the option Allow the computer to turn off this device to save power in the Device Manager must be deactivated In Windows 7 chlick on Start Control Panel Network and Sharing Center Double click on LAN Connection Click on the button Properties Select Internet Protocol version 4 TCP IPv4 Click on the button Properties Check the settings in the tab General The correct settings for the DHCP client are a Obtain an IP address automatically b Obtain DNS server address automatically Click on the button OK Connecting the Cables To avoid interference we recommend to operate the HPLC sys tem separate from the company network This is why we recom mend using a router A router has several LAN ports and one WAN port that can be used to integrate the LAN into a wide area network WAN e g a company network or the Internet On the other hand the LAN ports serve to set up a network from devices and a computer A patch cable is included in the accessories kit A router is not included in the scope of delivery To connect the router to a Detector 2550 User Manual V5193A Version 1 0 28 Legend Modules Router G LAN port WAN port Workstation Prerequisite Procedure Procedure Resul
15. extent for use in liquid chromatography Knowledge regarding the health risks of chemicals Participation during an installation of a device or a training by the company KNAUER or an authorized company If you do not belong to this or a comparable professional group you may not perform the work described in this user manual under any circumstances In this case please contact your supe rior Safety equipment When working with the device take measures according to lab regulations and wear protective clothing What must be taken into account KNAUER safety glasses with side protection protective gloves overall All safety instructions in the user manual The environmental installation and connection specifications in the user manual National and international regulations pertaining to labora tory work Original spare parts tools and eluents made or recom mended by KNAUER Good Laboratory Practice GLP Accident prevention regulations published by the accident insurance companies for laboratory work Filtration of substances under analysis Use of inline filters Once they have been used never re use capillaries in other areas of the HPLC system Only use a given PEEK fitting for one specific port and never re use it for other ports Always install new PEEK fittings on each separate port Follow KNAUER or manufacturer s instructions on caring for the columns Detector 2550 User Manual V5193A Version 1 0
16. means of the verti cal arrow keys Press AUTO ZERO key or hold the horizontal arrow key for approx 1 second to return to the main menu Creating a link A maximum of ten links between defined programs can be cre ated and saved Every link contains a program number P 01 19 a number R for repetitions 1 99 and a programmable wait status W 0 or 1 T 2 3 6 Select the Link menu Enter the link number Press horizontal arrow key to go to the editing mode of the submenu Move the cursor to position P in the display and enter the program number Move the cursor to position R R Repeat and enter the value number of repetitions for the specified program Move the cursor to position W in the display and enter 1 or 0 Note If the W 0 W Wait setting is used the detector waits for an external start signal or for the user to press number 1 run Every subsequent line starts Detector 2550 User Manual V5193A Version 1 0 49 Procedure KNAUER Controlling the detector immediately If the W 1 setting is used the link sequence is run without interruption Prog 4 Link Prog lt Link Prog L01 Setup Link lt line01 P02 R01 WO j GLP Fig 17 Creating a link Executing a link After the link has been executed the detector stops The display switches to the END mode LP 2 3 NOAUA Select the Link menu Enter the link number Press horizonta
17. of one another Practical tip Synchronization with an external clock is only possible when using the supplied ser vice tools which simplifies the setting when the time zone changes From the factory the device clock is set to Central European Time DISPLAY Here the brightness and contrast of the display can be set KNAUER Detector 2550 User Manual V5193A Version 1 0 40 Navigating through the GLP menu KNAUER Controlling the detector Controlling the GLP menu All displays of the GLP menu are for information purposes only They provide information about the use of the detector as well as an overview of the configuration and condition of the device Entries are not possible Note The supplied service tool records additionally all device data and is used for service remote diagnostics A diamond icon is displayed on the left of the lower line in the display Move the cursor onto the diamond icon to navi gate through the subitems of the GLP menu by means of the vertical arrow keys Press AUTO ZERO or hold the horizontal arrow key for approx 1 second to go to the main menu Structure of the GLP menu GLP S N ANA080200001 V01 10 GLP display D01 01 T00 85 GLP Service 10 11 2009 Instalation 05 12 2008 GLP Int max sig 0193 nW ref 0092 nW GLP Integration time 001 38 ms GLP Cal Grating 1177 Zero 0 1 GLP Holmium 360 9nm 361 5 gt o k GLP Holmium 446 2
18. performance liquid chromatography HPLC is a method for separating compounds determining substances and measur ing their concentration The UV detector is the most frequently used detector in HPLC The Smartline UV detector 2550 is a measuring system for fast and precise measurement of UV spectra and optionally with a halogen lamp for the visual spectrum The measurement prin ciple is based on the attenuation of a monochromatic light beam passing through liquid The self calibration of the detector guarantees operation with out external settings Type and combination of the installed lamps as well as the transmission of the corresponding installed flow cell are analyzed and used for the automatic setting of the integration time in order to achieve a high level of sensitivity Note This manual applies for the Smartline UV detector 2550 as well as for the UV Vis detector 2550 Use the device only for applications that fall within the range of the intended use Otherwise the protective and safety equip ment of the device could fail Local network and automatic config uration The detector is controlled either by means of the input field on the front of the device or by means of the chromatography software Normally the detector is controlled by the chromatography software through a local network LAN The detector connected to the local area network LAN is auto matically detected by the chromatography software When
19. port 29 Power connection 14 Power Supply 10 Power supply 14 power cable 10 power strip 10 R running a program 44 KNAUER Detector 2550 User Manual V5193A Version 1 0 75 Index S safety 9 Salts 8 SCAN 17 selecting the GLP menu 34 selecting the Link menu 34 selecting the Program menu 34 selecting the Setup menu 33 selection of the wavelength 32 service tool 53 Solvents 8 startup 19 storage 58 symbols 12 system messages 59 T technical data 63 Technical Support 13 53 terminal strip event and remote control 23 terminology 71 Transportation damages 70 Troubleshooting LAN 29 WwW WAKE UP program 47 Warranty 70 warranty conditions 72 KNAUER Detector 2550 User Manual V5193A Version 1 0 Wissenschaftliche Ger tebau Dr Ing Herbert Knauer GmbH All rights reserved Technical data are subject to change without notice Please check our website for latest updates and changes Translation of the original German edition of this manual version 1 0 Last manual update 2013 10 23 Printed in Germany on environmentally friendly paper from sustainable forests amp Smartline are registered trademarks of Wissenschaftliche Ger tebau Dr Ing Herbert Knauer GmbH P See up to date manuals online www knauer net downloads HPLC SMB Osmometry Wissenschaftliche Ger tebau Phone 49 30 809727 0 Dr Ing Herbert Knauer GmbH Telefax 49 30 8015010 KNALI E Hegauer Weg 38 E Mail infoGknauer
20. this been successful 8 Check that the IP port in the device s and chromatog raphy software are identical KNAUER Detector 2550 User Manual V5193A Version 1 0 31 Controlling the detector Controlling the detector Switching the detector on The device is switched on by the power key on the back of the device Prerequisite Installation has been completed Flow cell has been assembled CAUTION Possible damage to the device caused by con densed water Allow device to acclimate for 3 h before it is con nected to power supply and taken into operation After the devices has been switched on with the power switch on the rear the device name and with a delay the firmware version number appear on the display The device performs a self test which concludes with the message SYSTEM TEST OK The same procedure takes place when the device is switched on from standby mode The D lamp then preheats for ignition This is indicated by INI in the lower left of the display The start routine is completed with an automatic device calibration which is displayed with Calibrating After this ON appears in the display The basic settings are similar to those before switching the detector off The device is now ready for use Legend Q Display Program Link Event status Signal output dis play Arrow key Standby key amp Wavelength display Number key Time display G Status of program li
21. to act for 5 minutes 3 Flush with plenty of water 4 The cell is then dried in a nitrogen stream Note Do not use compressed air for drying as this often contains microscopic oil drops that can be depos ited onto the cell If flushing does not have the desired effect all flow cells can be disassembled to clean the lens Cleaning the lens of an analytical flow cell This description applies for the analytical flow cells A4061V2 A4042 and A4045 Device has been switched off Flow cell has been removed Detector 2550 User Manual V5193A Version 1 0 55 Legend PTFE seal ring Lens G Lens holder com pression part Threaded ring Procedure for cleaning the lens Prerequisite KNAUER Maintenance and care Loosen the threaded ring 4 with the 3 mm hexagonal span ner included with the flow cell Remove the lens holder 3 with tweezers or by gently tap ping and place on a clean surface The lens 2 is protected by a PTFE seal 1 This must be renewed every time the lens is disassembled Remove the lens and clean with a clean soft cloth or with an appropriate solvent in an ultrasonic bath Ensure that the clean lens is not touched by fingers Then place the flow cell back and ensure that the new PTFE seal does not interrupt the light path Next tighten the threaded ring with the hexagonal spanner in such a manner that damage to the lens is avoided Clea
22. 0 bar 0 5 mm A4069 1 16 0 8 mm 3 ul Stainless steel 250 ml min 200 bar 0 5mm A4095 1 16 0 8 mm 3 ul PEEK 250 ml min 100 bar KNAUER Detector 2550 User Manual V5193A Version 1 0 68 Accessories and Spare Parts KNAUER U Z View flow cells Technical data Path length Connection Inner diameter Volume Material Max flow rate Max pressure Path length Connection Inner diameter Volume Material Max flow rate Max pressure 10 mm 1 16 0 15 mm 0 18 ul Stainless steel 0 10 ml min 200 bar 10 mm 280 um 0 075 mm 0 045 ul Quartz glass 0 01 ml min 200 bar A4091 A4092 Flow cell replacement parts Foranalytical flow cells Lens for A4061V2 flow cell Lens for A4061 flow cell For preparative flow cells Mew Order number Fiber optics quartz glas rod N0076 Compression bushing P2644 PEEK spacer P2645 Stainless steel cover P2646 PTFE seal ring P7003 Detector 2550 User Manual V5193A Version 1 0 69 Accessories and Spare Parts Test Cells Test cell holmium oxide A4126 filter Test cell filter stray light A4146 KNAUER Detector 2550 User Manual V5193A Version 1 0 70 Legal Information Legal Information KNAUER Warranty Conditions The manufacturer s warranty for the device is valid for 12 months after the date of dispatch All warranty claims shall expire in the event that any unauthorized changes are made to the device Dur
23. 2550 User Manual V5193A Version 1 0 74 Index Index A abbreviations 71 Accessories and Spare Parts 65 Additives 8 ambient conditions 58 AUTO ZERO 17 automatic configuration 6 AVV marking 58 C Caring 54 CE marking see Declaration of conformity 72 changing a program 45 changing the optical path length 20 chromatography software 26 Cleaning 54 Connection Problems LAN 29 connector strip 22 Contact 13 53 controlling the detector 31 controlling the GLP menu 40 controlling the link menu 48 controlling the main menu 33 controlling the program menu 43 controlling the scan menu 51 controlling the set up menu 34 creating a link 48 creating a program 43 D Declaration of Conformity 72 declaration of conformity 72 Decontamination 11 decontamination 58 deleting a link 51 deleting a program 46 disposal 58 E electrical connections 22 Eluents 8 flammability 10 self ignition point 10 toxicity 10 error messages see system messages 59 executing a link 49 F features 7 flow cells available 66 H HPLC 6 installation 13 Installation location see location 13 IP port 29 L LAN Connection Problems 29 Router 28 Settings 27 Setup 27 Troubleshooting 29 Location requirements 13 M maintenance 53 Maintenance Contract 53 manual entry of control signals 25 Manufacturer 70 Modifiers 8 O Operating range 6 overview of buttons 17 P performing a wavelength scan 52 Port see IP
24. 4 connec tions KNAUER Detector 2550 User Manual V5193A Version 1 0 21 Startup Legend Threaded ring Q Stainless steel cover PEEK spacer Seal holder com pression bushing G Fiber optics with PTFE seal Fig 5 path length of a preparative flow cell Procedure for shorten 1 Loosen the threaded ring with a hexagonal spanner ing the path length 2 Remove stainless steel cover and the PEEK spacer 3 Insert the stainless steel cover again and tighten the threaded ring again The missing spacer causes the fiber optics to be pushed deeper into the flow cell 0 75 mm resulting in a shortened optical path length of 1 25 mm To further shorten down to 0 5 mm the PEEK spacer on the other cell side must also be removed Procedure for lengthen To increase the path length in steps of 0 75 mm put the spacers ing the path length back in 1 Loosen the threaded ring with a hexagonal spanner 2 Remove stainless steel cover and fiber optics together with the seal holder with tweezers 3 To enlarge the path length push the fiber optics out approx imately 1 mm Please use a clean cloth and avoid touching the fiber optics with your fingers 4 Put the fiber optics together with the seal holder back into the cell 5 Insert the PEEK spacer and then the stainless steel cover 6 Tighten the threaded ring again When tightening the threaded ring the rod shaped fiber optics is pushed into the correct position in th
25. AUTO ZERO or hold the horizontal arrow key for approx 1 second to return to the main menu Detector 2550 User Manual V5193A Version 1 0 35 Controlling the detector Structure of the Setup menu LAN manual Analog INTERFACE LAN DHCP RS232 9600 RS232 19200 RS232 38400 RS232 115200 IP adr 192 168 001 000 mask 255 255 255 000 IP GW 192 168 001 254 port 10001 D2 OFF D2 HAL ON HAL OFF HAL CAL zero 0 1 Grat 1175 auto calibration int sig int ref SIGNAL sig 0 7531 absorption ref 0 3789 TIME CONSTANT 1s 10s integrator 2 SCAN OUT integrator 1 411 nm s ANALOG OUT 1 1 000 au V 2 1 000 au V KNAUER Detector 2550 User Manual V5193A Version 1 0 36 Controlling the detector KNAUER ANALOG OUTOFFSET 1 2 0000 71mV 0000 72mV ANALOG IN set zero actual 000 nm FRACTION 0 00073au delay 074 0s event E 1 EVENTS active E 1 start 1 error 0 AZ 0 19 01 2010 d m y 16 00 46 h m s DISPLAY Brightness 1816 Contrast 0790 Fig 10 Structure of the Setup menu Parameters of the Setup menu INTERFACE Setting of the connection options of the detector Connections on the rear of the device IP adr mask Setting of the IP address and the subnet mask IP GW Port Setting of the gateway and the port LAMP Setting of the lamp configuration deute rium halogen or tandem A source that is not insta
26. Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig f Fi E ees Bo Eye 2 Be Oe es ee ee 73 Table of Figures gures Front VIEW id ne VA AU EE ea sat phe tele oe cheers et eae 16 Front view of the device with door open llle 16 R ar VIEW ores wed wees a alec cure T Reed esq deeem e Ree 18 installing the flow cell 4 243 4x Sr s SER UE RA RE E uA 20 path length of a preparative flow cell 0005 21 SDIIDG SUID sae uaea EXE CORERRAOUR E E CI ORAE d Re e os Ses 23 Terminal strip Event and remote control 00 23 Cabling system LAN se eons kek 4 ans ECCE UA ERE S 28 Displaycand keys oo ees Nowe Se Lace ex ades dog Ete d ud 31 Structure of the Setup menu 42a EX ds x ERE EY RA 36 Structure of the GLP menu cs uesoceda tk eR ERE he bees 41 Creating a program sesir qoe en paw ee Eee ee eee ar 44 RUNNING a rogatio uq RUE EE RUD RUE RU MORE ee RTE 45 Changing a program ex ERI RE Reik a Ses ose SE 46 Deleting apregraltiasso5ed04 a pisce ec ee neues gine 46 Creating a wakeup program 0 cece ee eee eee tees 47 Creating GIP sac ES pes Matha gee NERA RAT ee eS 49 Executing adios cf gna Lpxsst e XE ERE od end C E NO da 50 Deleting alin i eee e AUT Au OV e TR eras Mae dorm RAR 51 Analytical How cell 222 x RERSDRRE Heke renro prani 55 Fiber optics of a preparative flow cell llle 56 Replacing the flow cell 5a reU EXE Gd FERT CE 57 KNAUER Detector
27. RE Lee eRe eS a ve Ree es 48 Creating a NK vui es wees ee Ne AE WER a RRS eee ee d 48 Exec ting TK ves gid esee wide SEWER Pele a I ed ee eo ee 49 Deleting a ink sms xara PA AER a ie St Sala ak AL Aone IK oUt 51 Controlling the Scan menu 4 goes NS Ss RES p aV AD EACUS POUR Sea 51 Performing wavelength scans 0 ee eee ee eee eee eee 52 Maintenance and Care 1 ce ee ee hn 53 Contacting the Technical Support 2 2 6 ona ua goed bee oat a Beaded 53 Maintenance Contract s as cee daca tape Rok eee Mee Ree ee aed 53 Cleaning and Caring for the Device eee cee eee eee eee 54 Cleaning the flow cell 3 cae eS RR ee CR RR eee 54 Flushing the flow cell 1 5 0408 08 RR RE EE 54 Cleaning the lens of an analytical flow cell 0000000 54 Cleaning the fiber optics of a preparative flow cell 0 55 Replacing the flow cell 1 oe ess oye ex xe n hx RO X OR eas 57 Disposal uc senex v ittm v ave eA A bera DU E det adve eae fro 58 KNAUER Detector 2550 User Manual V5193A Version 1 0 Storage v boa EN A ert dco TO d Deane eae d pia p i os d ice dose ee 58 Troubleshooting an hdc 2a ox Hi Rer E PSU Sh EE SE edis 59 System messages x os Man bet e ea ete els bh es RES RU E Bis 59 Technical data aoo cannes cox e eS Oe ee eRe eS MRS eds 63 Accessories and Spare Parts 5 esa os n ake ow he 2E oS ok Rade eee 65 Available flow cells for the Smartline detector 2550 000 006 66 Anal
28. a an adjustable grating that must be moved for every wavelength Note X For each change of solvent the autozero scan should be performed again 1 Press SCAN to enter the Scan menu Detector 2550 User Manual V5193A Version 1 0 52 Procedure for wave length scans KNAUER Controlling the detector Press AUTO ZERO The detector scans the set wavelength range and sets all measured values to zero Wait until the main menu is shown in the display The autozero scan has now been completed Performing wavelength scans For a wavelength scan the UV spectrum of a sample is recorded The wavelength of light at the output gap is changed by turning the optical grating continuously During the procedure the light absorption is measured constantly T 2 Press SCAN to enter the Scan menu In the first line the signal sources absorption int sig or int ref can be selected The second line is used to specify the scan range in nanome ter Press SCAN key again The scan is executed immediately and saved The display returns to the main menu To subsequently output the saved scan proceed as follows 1 2 Press SCAN key again to enter the Scan menu Using the arrow keys position the cursor in the OUT field in the upper line Load Events 1 2 Signal 1 Signal 2 Prog off 0 on au au Prog lt SCAN absorption OUT Setup Link range 200 500 j GLP Hold 0 Time A1 A2 Run 1 min nm nm Pressi
29. al Support of KNAUER Take the device out of At any time take the device completely out of operation by operation either switching off the power switch or by pulling the power plug Opening the module The device may only be opened by the KNAUER Technical Sup port or any company authorized by KNAUER KNAUER Detector 2550 User Manual V5193A Version 1 0 11 Safety Definition of Personal and Material Damage Possible dangers related to the device are distinguished in per sonal and material damages in this user manual Type of damage Category Explanations Personal damages DANGER Lethal or very serious injuries can occur Personal and material CAUTION Moderate injuries can occur Device defects damage can occur Decontamination Contamination of devices with toxic infectious or radioactive substances poses a hazard for all persons during operation repair sale and disposal of a device DANGER Danger if getting in contact with toxic infectious or radio active substances Before disposing off or sending away contami nated devices commission an expert with the decontamination All contaminated devices must be properly decontaminated by a specialist company or the operating company before they can be recommissioned repaired sold or disposed of All materials or fluids used for decontamination must be col lected separately and disposed of properly KNAUER Detector 2550 User Manual V5193A Version 1 0 12 S
30. at do not con tain active elements are replaced with the character Use the horizontal arrow keys to navigate through the two lines of the display of the main menu With the number keys you can increase or decrease the number values With the vertical arrow keys you can increase or decrease the displayed values During navigation through the main menu the cursor is a flash ing rectangle at the current position During entry the cursor turns into the underscore character 1 Use the horizontal arrow keys to put the cursor at the desired position in the display 2 Use the number keys or the vertical arrow keys to enter a desired value Input mode is automatically terminated without saving the entered values if no key is pressed for 5 seconds Events M2 Signal 1 Signal 2 off0 oni A1 nm Selecting the Setup menu 1 Use the horizontal arrow keys to put the cursor in the top line of the display 2 Press the right horizontal arrow key for approx 1 second 3 Press AUTO ZERO or hold one of the horizontal arrow keys for approx 1 second to return to the main menu Detector 2550 User Manual V5193A Version 1 0 34 Procedure Procedure Procedure Navigating through the Setup menu Practical tip Increase or decrease number values KNAUER Controlling the detector Selecting the GLP menu 1 Use the horizontal arrow keys to put the cursor in the lower line of the display 2 Press th
31. change If one of the two events is used for fractionation then the behavior of the corresponding event fields in the main menu changes If the threshold is exceeded for fraction ation this setting prevents an event from being triggered When the threshold for fractionation is exceeded the assigned event switching is triggered Has no meaning in this case Detector 2550 User Manual V5193A Version 1 0 26 Network connection Note Process Note KNAUER Connecting the detector to other devices Control of the detector with chroma tography software The detector can be controlled within an LC system with a computer and with software e g KNAUER chromatography software OpenLab ChromGate or ClarityChrom The computer is connected via the LAN network connection or the serial interface RS 232 interface on the rear of the detector Note A maximum data rate of 80 Hz is available over the LAN interface The RS 232 interface is limited to a rate of 10 Hz This means that only the LAN interface can provide a sufficiently fast data transfer for full per formance of the device In combination with the software the Smartline detector 2550 can simultaneously measure up to four wavelengths and record an unlimited number of scans during the method run If the detector is controlled with software this is indicated in the display by amp symbol e g 3450 2030 240 400 For operation of the Smartl
32. e cell Inserting a spacer lengthens the optical path length by 0 75 mm When changing the path length the PTFE seal does not need to be changed KNAUER Detector 2550 User Manual V5193A Version 1 0 22 Connecting the detector to other devices Connecting the detector to other devices Prerequisites Tools KNAUER Electrical connections The detector is connected with the Events and remote control ter minal strip and within a network with the LAN connection to external devices A computer can also be connected to the detector using the RS 232 interface Connecting the connector strip with the cable To control one device through another you use the pin header The single ports are used to exchange control signals To use remote control you have to connect cables to the pin header Therefore connect the cables firstly to the spring strip and push the spring contacts of the spring strip secondly onto the pins of the pin header The device has been turned off The power plug has been pulled Depressor tool CAUTION Short circuit hazard Turn off the device before connecting it to the pin header Pull the power plug CAUTION Electrostatic discharge can destroy the electronics Wear protective bracelet against electrostatic dis charge and ground Detector 2550 User Manual V5193A Version 1 0 23 Connecting the detector to other devices Procedure Place the spring strip on a suitable surface
33. e flow cells for the Smartline detector 2550 Analytical flow cells Technical data Path length Connection Inner diameter Volume Material Max flow rate Max pressure Path length Connection Inner diameter Volume Material Max flow rate Max pressure Path length Connection Inner diameter Volume Material Max flow rate Max pressure 10 mm 1 16 1 1 mm 10 ul Stainless steel with heat exchanger 20 ml min 300 bar 3 mm 1 16 1 0 mm 2 ul Stainless steel 50 ml min 300 bar 3mm 1 16 1 0 mm 2 ul PEEK 50 ml min 30 bar A4061V2 A4042 A4045 Detector 2550 User Manual V5193A Version 1 0 67 Accessories and Spare Parts Preparative flow cells Technical data Path length Connection Inner diameter Volume Material Max flow rate Max pressure Path length Connection Inner diameter Volume Material Max flow rate Max pressure Path length Connection Inner diameter Volume Material Max flow rate Max pressure Path length Connection Inner diameter Volume Material Max flow rate Max pressure Path length Connection Inner diameter Volume Material Max flow rate Max pressure 0 5 1 25 2mm A4066 1 8 2 3mm 1 7 4 3 6 8 ul Stainless steel 1000 ml min 200 bar 0 5 1 25 2 mm A4067 1 8 2 3mm 1 7 4 3 6 8 ul PEEK 1000 ml min 100 bar 0 5 1 25 2mm A4068 1 4 4 0 mm 1 7 4 3 6 8 ul Stainless steel 10000 ml min 20
34. e of your HPLC device will ensure successful analyses and reproducible results Contacting the Technical Support You have various options to contact the Technical Support 49 30 809727 111 49 30 8015010 support knauer net You can make your requests in English and German Maintenance Contract The following maintenance work on the device may only be per formed by the manufacturer or a company authorized by the manufacturer and is covered by a separate maintenance con tract Opening the device Removing the hood or the side panels Detector 2550 User Manual V5193A Version 1 0 54 Procedure Prerequisite KNAUER Maintenance and care Cleaning and Caring for the Device CAUTION Intruding liquids can cause damage to the device Place solvent bottles next to the device or in a solvent tray Moisten the cleaning cloth only slightly All smooth surfaces of the device can be cleaned with a mild commercially available cleaning solution or with isopropanol Cleaning the flow cell Increased noise of the base line and reduced sensitivity can be a result of a dirty flow cell Often it is sufficient to flush the flow cell to restore optimal sensi tivity Flushing the flow cell The following solvent is recommended for flushing 1 mol l HCI 1 mol l NaOH Ethanol Acetone Water 1 Fill one of the recommended solvents into a syringe 2 Inject it into the inlet of the flow cell and allow it
35. e right horizontal arrow key for approx 1 second 3 Press AUTO ZERO or hold the horizontal arrow key for approx 1 second to return to the main menu Selecting the Link menu 1 Use the horizontal arrow keys to put the cursor in the lower line of the display 2 Press the left horizontal arrow key for approx 1 second 3 Press AUTO ZERO or hold the horizontal arrow key for approx 1 second to return to the main menu Selecting the Program menu 1 Use the horizontal arrow keys to put the cursor in the top line of the display 2 Press the left horizontal arrow key for approx 1 second 3 Press AUTO ZERO or hold the horizontal arrow key for approx 1 second to return to the main menu Controlling the Setup menu In the SETUP menu fundamental parameters for controlling the detector are specified Usethe horizontal arrow keys to navigate through the two lines of the display Move the cursor onto the diamond icon to navigate through the subitems of the Setup menu by means of the vertical arrow keys With the vertical arrow keys you can increase or decrease the number values 1 Use the horizontal arrow keys to put the cursor at the desired position in the display 2 Use the number keys to enter a desired value 3 Use the vertical arrow keys to select the option 4 Move the cursor onto the diamond icon to navigate through the further subitems of the Setup menu by means of the ver tical arrow keys 5 Press
36. ed go through the following points Check after each point if the problem is solved If you did not manage to locate the problem call the Technical Support 1 Check the status of the LAN connection in the Win dows taskbar faj Connected sl Connection not established If no connection was established test the following Is the router on Is the patch cable connected correctly to the router and the computer Check the router settings Is the router set to DCHP server Is the IP address range sufficient for all the connected devices Check all connections Are the patch cable connected to the LAN ports and not the WAN port Are all cable connections between devices and router correct Are the cables plugged in tightly Detector 2550 User Manual V5193A Version 1 0 30 Connecting the detector to other devices 4 If the router is integrated into a company network pull out the patch cable from the WAN port Can the devices communicate with the computer even though the router is disconnected from the com pany network 5 n case you own a Control Unit check the settings in the menu Setup Network s LAN DHCP set for controlling Did the device receive an IP address 6 Turn off all devices router and computer Firstly turn on the router secondly the devices and the computer Has this been successful 7 Replace the patch cable to the device with that no connection could be established Has
37. error Restart the device If the system message appears again notify KNAUER Technical Support I2C failed for Restart the device lamp s If the system message appears again notify KNAUER Technical Support KNAUER Detector 2550 User Manual V5193A Version 1 0 61 Troubleshooting System message I2C failed for panel I2C Init failed I2C operation failed Instrument remote controlled Insufficient access Invalid command Invalid parameter s Invalid time in time table Invalid time table index Link is loaded Memory error No link available No link available Pls edit link first No space for scan is available No stored scans available No time table to start Restart the device If the system message appears again notify KNAUER Technical Support Restart the device If the system message appears again notify KNAUER Technical Support Restart the device If the system message appears again notify KNAUER Technical Support This entry is not executable Quit software program Change the entry Check the cable connections Change the entry Check the validity of the parameters Change the entry in the program line First unload the link then change the link or delete it Wait until the link has been executed then change the link or delete it Restart the device If the system message appears again notify KNAUER Technical Support Create a link and edit it Create a l
38. ess than 100 ms The beam guidance is designed so that no chromatic aberra tion occurs and thus the best possible useful signal is avail able independent of the selected wavelength Automatic recording and storage of the device specific char acteristics that are important for GLP Good Laboratory Prac tice and OQ Operation Qualification or are of interest for the device service and device history These are operating times operating parameters wavelength accuracy and refer ence spectra Self running and up to date device status diagnostics Control with KNAUER HPLC software possible Easy integration of the detector into complex chromatogra phy systems Extremely low noise level and equally low drift High data rates for fast chromatography Flexible options for use in the entire field of LC applications due to a comprehensive range of flow cells for the KNAUER detectors from nano HPLC cells with flow rates 100 nl min to preparative flow cel Is with 10 l min Various types of flow cells are available to the user Pay attention to the compatibility of the flow cells A test cell is preassembled to the detector Before the detector can be used for measurements the test cell has to be replaced by a flow cell Detector 2550 User Manual V5193A Version 1 0 1 8 Note Suitable eluents Acetone at 4 25 C 39 2 77 0 F Acetonitrile Benzene Chloroform Ethyl acetate Ethanol Hexane heptane at 4 25
39. et Detector 2550 User Manual V5193A Version 1 0 16 Device overview Front view of the device The input panel and display of the detector are located in the door on the front of the device Behind the door there is the holder for the flow cell with cover plate Device door with input field and display Legend Q Display with upper and lower status bar G AUTO ZERO key Number keys SCAN key Arrow keys Standby key with LED display Fig 1 Front view Front view of the device with door open Legend Knurled head screws Cover plate with sensor G Carriage holder for flow cell Fig 2 Front view of the device with door open KNAUER Detector 2550 User Manual V5193A Version 1 0 17 Device overview Overview of the basic buttons Vertical The vertical arrow keys serve arrow keys to modify the corresponding controlled parameters They are not used to move between the upper and lower status bar of the display The upper vertical arrow key also has a special function Once an error message appears in the display it can be deleted by pressing the N arrow key Horizontal The operation of the arrow keys horizontal arrow keys moves the cursor to the individual input or switching fields and confirms an entry or selection If you hold these keys down you will switch to one of the menus shown to the side of the display Prog Link Setup GLP AUTO ZERO Actuating this
40. etup Link END 030 00 240 400 jJ GLP Hold 0 Time A1 A2 Run 1 min nm nm Fig 13 Running a program Changing a program When values are entered the old values are overwritten Procedure 1 Select the Program menu 2 Select the program number 3 Press horizontal arrow key to go to the editing mode of the submenu 4 Using the vertical arrow keys navigate to the program line that is to be changed 5 Enter the desired value KNAUER Detector 2550 User Manual V5193A Version 1 0 46 Controlling the detector 6 Position cursor on the diamond icon and hold the horizontal arrow key down to exit the submenu Load Events 2 Signal 1 Signal 2 Prog off on 1 au au Prog 4 Open PO 01 02 03 p Setup Link lt p GLP Hold 0 Time M 2 Run 1 min nm nm Load Events 12 Signal 1 Signal 2 Prog off0 on 1 au au Prog P02 00 ye Setup Link 000 0 240 000 GLP Hold 0 Time A1 2 Run 1 min nm nm Fig 14 Changing a program Deleting a program Procedure 1 Select the Program menu 2 Enter program number 0 3 Press horizontal arrow key to go to the editing mode of the submenu 4 Enter number of the program to be deleted 5 Press vertical arrow key to confirm Note X Running programs and programs which are contained in a running link cannot be deleted Load Events 2 Signal 1 Signal 2 Prog offO on 1 au au Prog 4 Open PO 01 02 03
41. g material 235123 x a rere towers e e BOE Re ee PaO as 14 Protective film on the display 0 0 ce eee ee eee eee ee eee 14 Scope Of SUPDIY s e ee tee ex e t d boleh RE es 15 Checking the scope of supply cee eee ee ce eee eee eee 15 DEVICE OVerVIEW s cdd does Be Cy Sek die acie Me hah ewe dal edu 16 Front view at the device es Lg as Note Aou atas ola Became eee aks e DO d RES d anat 16 Device door with input field and display llle 16 Front view of the device with door open 2 eee eee eee 16 Overview of the basic buttons 0 0 cee ee eee ee eee tees 17 Rear view of the device cee ce et eee eee ete nees 18 Stan coss ctae wate et es ex eee TAG Pe ne pee eS 19 Checklist before initial operation 0 eee ee eee eee 19 Installation of the flow cells 25 4450 ert let RU s edt exe nak ea 19 Installing the flow cell 4s oA ec thera dig o d cot CE n 20 Changing the path length of a preparative flow cell 20 Connecting the detector to other devices 0065 22 KNAUER Detector 2550 User Manual V5193A Version 1 0 Electrical connections 1 x eed hex EAT ER ER EN E et e d 22 Connecting the connector strip with the cable 0 0 22 Terminal strip Event and remote control on the rear of the device 23 Manual configuration of the control signals events 25 Control of the detector with chromatog
42. gest acces sible wavelength corresponds thus to a control voltage of 9 V Detector 2550 User Manual V5193A Version 1 0 39 Controlling the detector FRACTION In order to activate ports 1 and 2 on back of the detector for fraction collecting event 1 must first be activated from the main screen The corresponding port 1 or 2 must also be selected in the Fraction collection menu together with a thresh old value AU and optionally a delay time When the threshold value is reached exceeded the selected port is opened and the event field remains active on the main screen When the signal falls below the threshold the port is closed and the event field remains active on the main screen In order to activate ports 3 and 4 on back of the detector for fraction collecting the corre sponding port 3 or 4 must be selected in the Fraction collection menu together with a threshold value AU and optionally a delay time When the threshold value is reached exceeded the selected port is opened and the event field remains inac tive on the main screen When the signal falls below the threshold the port is closed and the event field remains inac tive on the main screen EVENTS Used for testing the terminal strip func tions The connections E 1 to E 3 and the 24 V output can be selected which can be activated individually DATE Setting of the time and date Day month year hour minute and second are set independently
43. ine detector 2550 using ChromGate or ClarityChrom no special settings are necessary on the detec tor Connecting a Device in a Local Area Network LAN to a Computer This chapter describes how to set up a local area network LAN and how a network administrator can integrate this LAN into a company network The description applies to the operating sys tem Windows and all conventional routers To set up a LAN we recommend to use a router The following steps are necessary 1 On the computer go to the Control Panel and check the LAN properties 2 Connect the router to the devices and the computer On the computer configure the router to set up the network A w Install the chromatography software from the data storage device 5 Switch on the device and run the chromatography software The port ist set to 10001 at the factory The IP port numbers in the device configuration of the chromatography software must be identical to those in the device otherwise the connection fails Detector 2550 User Manual V5193A Version 1 0 27 Prerequisite Procedure Note KNAUER Connecting the detector to other devices Configuring the LAN Settings The LAN uses only one server which is normally the router from that the devices automatically receive their IP address Nn BW MN 7 In Windows power saving hibernation standby automatic Windows update and screen saver must be deactived In case you use a
44. ing the warranty period any components with material or design related defects will be replaced or repaired by the manu facturer free of charge This warranty excludes the following 1 Accidental or willful damage 2 Damage or errors caused by third parties that are not con tractually related to the manufacturer at the time the damage occurs 3 Wear parts fuses glass parts columns light sources cuvettes and other optical components 4 Damage caused by negligence or improper operation of the device and damage caused by clogged capillaries 5 Packaging and transport damage In the event of device malfunctions directly contact the manu facturer Wissenschaftliche Ger tebau Dr Ing Herbert Knauer GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 0 Fax 49 30 8015010 E Mail info knauer net Internet www knauer net Transportation Damages The packaging of our devices provides the best possible protec tion against transportation damage Check the devices for signs of transportation damages In case you notice any damage con tact the Technical Support and the forwarder company within three workdays Detector 2550 User Manual V5193A Version 1 0 71 Abbreviations and terminology Abbreviations and terminology Here you can find information on the abbreviations and termi nology used in this device manual for the Smartline detector 2550 Terminology Explanations GLP Good Laboratory Practice
45. ink and edit it Check the detector Check the number of program lines A maximum of 99 program lines are possible Edit the data by means of the chromatography software Save the scan Edit the data by means of the chromatography software KNAUER Detector 2550 User Manual V5193A Version 1 0 62 KNAUER Troubleshooting Not enough Check the detector Check the number of space to store program lines A maximum of 99 program link lines are possible Not enough Check the detector Check the number of space to store program lines A maximum of 99 program program lines are possible Not supported Change the entry Program does Create a program not exist Program is Quit program or wait until program has running been completed Scan is already Cancel scan procedure or wait until scan active procedure has been completed Thislinkisusedin First quit or delete wakeup program Wake up wu 2 Wake Up then edit or delete link This program is First pause or delete link then edit or used in a link delete data by means of the chromatography software This program is First quit or delete wakeup program used in Wake up wu Wake Up then edit or delete data by means of the chromatography software Time already Correct the time entry exists Time table is not The device is in Standalone mode no active program is running If you try to quit a non existent program sequence this message appears Time tab
46. ke sure the room is well ventilated Keep clear at least 15 cm at rear and 5 10 cm at each side for air circulation Space Requirements Side clearance to other devices At least 5 cm if there is another device on one side At least 10 cm if there are devices set up on both sides Atleast 15 cm on the rear panel for the fan Detector 2550 User Manual V5193A Version 1 0 14 Note Checking for signs of damage during trans port Remove fixation material Removing device from packaging KNAUER Unpacking and Setup Power Supply and Connection The device is intended for use with AC power networks of 100 240 V The supplied power cable is to be used to connect the device to the mains supply Only use power units with a permission for use from your coun try In case of queries contact the Technical Support Power Cable Only the supplied power cable is to be used to connect the device to the mains supply Replace defective power cables only with original accessories from KNAUER Power Plug Make sure that the power plug on the rear of the device is always accessible so that the device can be disconnected from the power supply Packaging and transport At the factory all KNAUER devices are carefully packed for safe transport Check the device and accessories for signs of damage that occurred during transport If the shipment is incomplete or damaged inform the manufacturing factory within three
47. key trig key gers a signal auto zero for the currently set wavelength If the display shows the SCAN menu then press ing the AUTO ZERO key will effect an auto zero scan over the entire wavelength range With the AUTO ZERO key you return to the main menu SCAN key With this key you switch to the SCAN menu Pressing it a second time triggers a scan of the selected wavelength range KNAUER Detector 2550 User Manual V5193A Version 1 0 18 Legend Serial number and manufacturer G Analog outputs scalable G LAN network con nection Serial interface G Fan opening Terminal strip Event and remote control Power switch Power connection KNAUER Device overview er E All ten number keys make possible entry of numeric values at the cursor position Pressing the standby key for two seconds puts the detector into a power saving mode Event inputs and communication interfaces remain active The standby status is indicated by the lit red LED above this key To switch back on press the standby key briefly Calibration is not performed in this case as the set wavelength has been fixed Note To disconnect the detector from the mains power press the power switch on the rear of the device Rear view of the device The rear of the device contains the mains power connection power switch connections for external devices technical symbols and serial number
48. l arrow key to go to the editing mode of the submenu Press AUTO ZERO to go to ON OFF mode Enter the link number Press number 1 The display switches to HOLD mode Press number 1 again The display switches to RUN mode linked program is running Detector 2550 User Manual V5193A Version 1 0 50 Controlling the detector 8 After the link has been completed press AUTO ZERO The display switches to ON OFF mode Load Events 1 2 Signal 1 Signal 2 Prog offO on 1 au au Prog Open L01 pSetup Link J gt GLE Hold 0 Time Aq A2 Run 1 min nm nm Load Events _ _2 Signal 1 Signal 2 Prog off 0 on 1 au au Prog 4 L01 p Setup Link line01 PO1 RO1 WO jJ GLP Hold 0 Time A1 A2 Run 1 min nm nm Load Events 1 2 Signal 1 Signal 2 Prog off on1 au au Prog P01 00 2030 0109 Setup Link HOLD 000 00 240 400 j GLP Hold 0 Time A1 A2 Run 1 min nm nm Load Events 2 Signal 1 Signal 2 Prog off on 1 au au Prog P01 00 2030 0109 Setup Link lt RUN 019 00 240 400 jJ GLP Hold 0 Time Aq A2 Run 1 min nm nm Load Events 2 Signal 1 Signal 2 Prog offO0 on 1 au au Prog 4 P01 00 2030 0109 Setup Link lt END 030 00 240 400 j GLP Hold 0 Time A1 A2 Run 1 min nm nm Fig 18 Executing a link KNAUER Detector 2550 User Manual V5193A Version 1 0 51 Procedure Autozer
49. le is not First load the program then start the loaded program Time table line is Edit the program line empty Too many linesin Check the number of program lines program A maximum of 99 program lines are possible Wrong Line Change the entry in the program line number Detector 2550 User Manual V5193A Version 1 0 63 Technical data Technical data Smartline detector 2550 Detector type Variable multiple wavelength UV VIS detector Detection channels 4 software 2 standalone Light source Deuterium D2 with integrated GLP chip one lamp version Deuterium D2 and halogen lamps with integrated GLP chip two lamp ver sion Wavelength range 190 750 nm one lamp version 190 900 nm two lamp version Spectral bandwith 6 nm at Hg line FWHM Wavelength accu 2 0 nm verification with integrated racy holmium oxide filter Wavelength preci 0 4 nm ASTM E1657 98 sion Noise 0 75 x 10 AU at 254 nm ASTM E1657 98 Drift 1 5 x 10 AU h at 254 nm ASTM E1657 98 Linearity 2 2 AU at 270 nm ASTM E1657 98 Time constants 0 1 0 2 0 5 1 0 2 0 5 0 10 0 s Maximum data 80 Hz LAN Analog 1 channel rate 10 Hz RS 232 1 channel Input Error IN Start IN Autozero 0 10 V Analog IN Output Events 1 3 5 V 24 V Value Analog output 2x0 5V scalable 20 bit offset adjust able Control Digital LAN LAN DHCP RS 232 remote control Analog wavelength control Manual fr
50. lled is indicated by Detector 2550 User Manual V5193A Version 1 0 37 Controlling the detector CAL Display of the effective line density of the optical grating in lines mm and offset of the unbent light Necessary for automatic wavelength accuracy and correction thereof Manual setting not necessary automatic calibration is started with the vertical arrow keys and initiates a com plete calibration procedure Note Itis recommended that after each change of the flow cell or solvent an auto calibration should be performed to opti mally adjust the detector pro perties When doing so make sure that the flow cell is comple tely filled with solvent and free of air bubbles SIGNAL Selection of the signal source that will be used for all outputs Selection between absorption int sig signal channel and int ref reference channel TIME CONSTANT Selection of a time constant to be used for signal smoothing The larger the value of the selected time constant the stronger the applied signal smoothing The sur faces of the elution peaks remain their profile can however be changed in partic ular with long time constants SCAN OUT Adjustment of the output speed of a pre viously recorded spectrum of the down stream recording device and the specification of the analog output over which the spectrum is output The per missible spectral range is 190 900 nm ANALOG OUT Scaling of the two analog outputs accord ing to
51. makes it possible to supply a consumer switched with event 1 relay The output is protected with an internal 62 mA fuse no Reference point forthe Lieve 24 V valve Event controlled switching of 24 V against GND The output is protected with an internal 250 mA fuse This output can be used for direct opera tion of a fraction valve External A Allows external analog control of the detector when the option ANALOG has been selected in the SETUP menu The control voltage must be applied against AGND KNAUER Detector 2550 User Manual V5193A Version 1 0 25 Procedure KNAUER Connecting the detector to other devices AGND Reference point of the voltage at the input External A Manual configuration of the control signals events In the program menu the events E 1 and E 2 can be activated directly and independent of one another Here it is relevant for the behavior whether or not E 1 or E 2 has been selected as con trol event in the FRACTION menu see Setup menu 1 Open the Program menu 2 Select the program number 3 Press horizontal arrow key to go to the editing mode of the submenu Enter values for events in the upper status bar by using the verti cal arrow keys If none of the two events is used for fractionation they have the following meaning Hc Event output remains in off state off Event output is switched fh The output is switched on for 1 second and then off again E No status
52. net D 14163 Berlin Germany Internet www knauer net KNAUER 2013 V5193A 0 05 9 13 Koe
53. ng one of the vertical arrow keys activates the scan out put The progress of the output is indicated by the continu ing rise in the upper wavelength Load Events 1 2 Signal 1 Signal 2 Prog off on 1 au au Prog lt SCAN OUT Setup Link range 200 386 j gt GLP Hold 0 Time A1 A2 Run 1 min nm nm Detector 2550 User Manual V5193A Version 1 0 53 Maintenance and care Maintenance and care Phone Fax E mail KNAUER that work areas are always well ventilated When performing maintenance tasks on the device always wear safety glasses with side protection protective gloves and an overall All wetter components of a device e g flow cells of detectors or pump heads and pressure sensors for pumps have to be flushed with isopropanol first and water afterwards before being main tained disassembled or disposed WARNING AN Irritation of retina through UV light Concen trated UV light can leak out from the flow cell or the fiber optic connectors Switch off the device and pull the power plug CAUTION Performing maintenance tasks on a switched on device can cause damage to the device Switch off the device and pull the power plug Users may perform the following maintenance tasks themselves Regularly check the light intensity of the D5 lamp best before 2000 operating hours Inspect the flow cell assembly Clean the flow cell Replace the flow cell Proper maintenanc
54. ning the fiber optics of a preparative flow cell This description applies for the preparative flow cells A4066 A4067 A4068 and A4069 The preparative flow cells have a rod shaped fiber optic instead of the concave lens of the analytical cells Device has been switched off Flow cell has been removed Detector 2550 User Manual V5193A Version 1 0 56 Maintenance and care Legend Threaded ring Q Stainless steel cover PEEK spacer Seal holder com pression bushing G fiber optics with PTFE seal Fig 21 Fiber optics of a preparative flow cell Procedure for cleaning 1 Loosen the threaded ring with the 3 mm hexagonal spanner the fiber optics included with the flow cell 2 Remove stainless steel cover and the PEEK spacer not part of A4069 3 Using tweezers remove the holder with the fiber optics 4 Carefully push the fiber optics out of the holder and wipe off the PTFE seal The PTFE seal must be renewed every time the fiber optics are cleaned 5 Clean the fiber optics with a clean soft cloth or with an appropriate solvent in an ultrasonic bath Ensure that the clean fiber optics are not touched by fingers 6 Then place the flow cell back and ensure that the new PTFE seal does not interrupt the light path 7 Next tighten the threaded ring with the hexagonal spanner in such a manner that damage to the lens is avoided Result The fiber optics have been cleaned and put back into the flow cell
55. nk SCAN key AUTO ZERO key Fig 9 Display and keys KNAUER Detector 2550 User Manual V5193A Version 1 0 32 Procedure for switching the device on Procedure for selecting the wavelength Procedure for measure ment configuration KNAUER Controlling the detector 1 Switch the detector on with the power switch on the rear of the device The device name is displayed followed by the firmware version number 2 Wait until the detector has performed the system test This is indicated with the message SYSTEM TEST OK 3 Wait until deuterium lamp has preheated The preheating is indicated by INI in the lower left of the display 4 Wait until the device calibration is completed ON then appears in the display Selecting the wavelength 1 Using the arrow keys position the cursor in one of the two fields for the wavelength 6 2 Enter the desired value using the number keys or using the vertical arrow keys 3 If only one wavelength is to be used then a zero must be entered in the unused field This deactivates the correspond ing detector channel 4 Confirm entry by using the horizontal arrow keys Otherwise the display will return to the previous value after five seconds Two spectral edge filters to suppress the second diffraction order are automatically activated if a deuterium lamp is installed and the shortest wavelength in all active channels exceeds an inter nal default value The activated filte
56. nm 446 7 gt o k GLP H beta 486 0nm 486 2 o k Detector 2550 User Manual V5193A Version 1 0 41 Controlling the detector KNAUER Fig 11 GLP Optical BW 5 0nm Stray light 3 2au GLP D2 S N SFA082207683 starts 0217 00374 1h GLP Hal S N SND092800004 00232 3h GLP Time total 00582 8h motor 00000 9h Structure of the GLP menu Detector 2550 User Manual V5193A Version 1 0 42 Controlling the detector Parameters of the GLP menu vae Meng E GLP S N Display of the serial number and the cur rently installed firmware version GLP display Display of the firmware version GLP Service Display of the initial installation and last Installation service GLP Int max Display of the maximum light output in the measurement and reference channel This specification serves mainly to evalu ate the lamp aging This value is deter mined during device calibration and thus does not change during the work phase of the detector but only when calibration is performed again GLP Integration Display of the optimal integration time of time the detector which is automatically determined during calibration The value is dependent on the status of the lamps and the flow cell that is used GLP Cal Display of the line density of the optical grating as well as the fine deviation of the zeroth spectral order Both values are used for exact positioning on the selected wavelength GLP
57. o scan Procedure for the autoz ero scan KNAUER Controlling the detector Deleting a link 1 Select the Link menu 2 Enter link number 0 3 Press horizontal arrow key to go to the editing mode of the submenu 4 Enter number of the link to be deleted 5 Press vertical arrow key to confirm Note Running links cannot be deleted Load Events 2 Signal 1 Signal 2 Prog offO on 1 au au Prog 4 Open LO 01 02 03 p Setup Link lt GLP Hold 0 Time 1 A2 Run 1 min nm nm Load Events 2 Signal 1 Signal 2 Prog off0 on 1 au fau Prog Delete link L gt Setup Link GLP Hold 0 Time A1 A2 Run 1 min nm nm Load Events 2 Signal 1 Signal 2 Prog off 0 on 1 au au Prog Delete link L01 Setup Link confirm with up down key GLP Hold 0 Time 1 A2 Run 1 min nm nm Fig 19 Deleting a link Controlling the Scan menu The Scan menu enables the following functions Selection of the wavelength range for the scan and the autoz ero scan Output of the saved scan data The Smartline detector 2550 can save a scanned spectrum and output it via the analog output 1 or 2 selected in the Setup menu Before recording spectra the detector must perform the so called autozero scan During this procedure the solvent spec trum is measured and the measurements along the entire set wavelength range are set to zero This can take some time as the wavelength is set vi
58. ont panel Programming Timed wavelengths events fraction valves links wake up program link 19 programs 99 program lines KNAUER Detector 2550 User Manual V5193A Version 1 0 64 Technical data tion operating hours lamp s operating hours step motor operating hours number of lamp ignitions Power consump 8W tion in standby mode Dimensions in mm 226 x 135 x 410 mm width x height x depth Height above sea maximum 2000 meters level GLP Detailed report including lamp recogni KNAUER Detector 2550 User Manual V5193A Version 1 0 65 Accessories and Spare Parts Accessories and Spare Parts For repeat orders of spare parts use the enclosed packing list Contact the Technical Support in case there are any questions on spare parts or accessories Devices and accessories Name Order number number UV VIS detector 2550 without E 91XA flow cell two lamp version Smartline UV detector 2550 without flow A5190XA cell one lamp version Device manual English V5193A Device manual German V5193 Power supply cable 230 V M1479 LAN network cable A5255 Ethernet patch cable CAT5e Re order Name Order number Complete accessory kit F5190 Deuterium lamp one lamp device A5193 HBST deuterium lamp for UV Vis detector A5194 two lamp version Halogen lamp A5195 KNAUER Detector 2550 User Manual V5193A Version 1 0 66 KNAUER Accessories and Spare Parts Availabl
59. port Detector 2550 User Manual V5193A Version 1 0 60 Troubleshooting Cannot read RTC Restart the device If the system message appears again notify KNAUER Technical Support Cannot set Modify the entry acquisition parameters Cannotstarttime Edit the data by means of the table chromatography software Cannot write Restart the device data on FRAM If the system message appears again notify KNAUER Technical Support CRC failed Restart the device If the system message appears again notify KNAUER Technical Support D2 Lamp read If the system message appears again failure Ignition notify KNAUER Technical Support counter The lamp has to be replaced D2 Lamp read If the system message appears again Ignition counter notify KNAUER Technical Support The lamp has to be replaced D2 Lamp read If the system message appears again lamp lifetime notify KNAUER Technical Support The lamp has to be replaced D2 Lamp does Switch off lamp on touchscreen and turn it not start on again If the system message appears again notify KNAUER Technical Support The lamp unit has to be replaced Data acquisition Noentries are possible First stop acquiring active measurement data afterwards you can make a new entry Error input Check the external devices and cable activated connections Error Restart the device programming If the system message appears again flash notify KNAUER Technical Support Filter move
60. r is indicated by the raised in front of the specification of the wavelength Options for signal output The output of a measurement can be configured 1 Position the cursor in one of the two fields for the signal out put 3 2 Use the vertical arrow keys to select the desired option Possible options are The signal is inverted d The display of signal 1 is divided by signal 2 udm The display of signal 2 is divided by signal 1 The absorption ratio of the measurement can thus be generated to check the chromatographic separation quality at two wave Detector 2550 User Manual V5193A Version 1 0 33 Navigation through the main menu Practical tip Procedure for entering values Legend Setup menu GLP menu G Link menu Program menu Procedure KNAUER Controlling the detector lengths The ratio values are displayed by the symbols 12 or 21 at the decimal point of the absorption value Load Events _ _2 Signal 1 Signal 2 Prog off on 1 au au Prog Open 00 22030 210109 Setup Link ON 240 400 jJ GLP Hold 0 Time 1 A2 Run 1 min nm nm Controlling the main menu The normal display after switching the device on shows the last set operating mode The flashing cursor is in the lower left cor ner of the display The last loaded program or link is shown in the upper left Time event setting and wavelength correspond to the first line of the loaded program All fields th
61. raphy software 26 Connecting a Device in a Local Area Network LAN to a Computer 26 Controlling the detector 2 60 5 545 45 655 ERE ERE SERE 31 Switching the detector Ohr es IER RICO ERR RR RU WR S RUN ERAI 31 Selecting the wavelength ves iaces I REORRLE RT RERO A EA TRUE Es 32 Options Tor signal output 4 5 4 oda the eee eee x exe EA 32 Controlling the main menu 4o o cox ee pe ES Ba Rt Ie Seles Rea 33 Selecting the Setup menu o ced e c RR UP e o eee eats 33 Selecting the GLP menu eost per S RR ECOSSE RU A 34 Selecting the Link menu scs ey e E eee O me E ER 34 Selecting the Program menu usse er Wee PS eRe EKG RES 34 Controlling the Setup menu 4 er cree ER ERR ER ee Rs 34 Structure of the Setup merui a Soe ewe RO eae e ea a ER es 35 Parameters of the Setup menu 0 eee eee eee eee eens 36 Controlling the GLP menu sce wegen ae tenes ago oan Kegs ee doge 40 Structiire OF the GLP menu esi t aeta deste b a hee dirae 40 Parameters of the GLP menu dee dere ERRARE WAR ELE Y 42 Controlling the Program menu eee ee eect teen tees 43 Creating a programi essa bed spi CREDO HK ERE E AUN 43 Running a program vios uas Fes t e d SA ea este tag thes 44 Changing a program 3e d aa ee sat Y Sp RO SO ORAS TRESS 45 DeletinigraprOgralfi 2 3 1 aea teure rema E artt c A e hee urs 46 Creating a program with preselectable start time 47 Controlling the Link menu 3es so oes REX ME
62. s can be cre ated and saved Programs can be created for the following functions Specification or modification of the wavelengths Definition of signal events during a wavelength program Creating a program 1 Select the Program menu 2 Select the program number 3 Press horizontal arrow key to go to the editing mode of the submenu 4 Enter desired values 5 To specify the desired start time in the submenu position the cursor on the diamond icon and press the vertical arrow keys 6 Press AUTO ZERO key to return to the main menu Detector 2550 User Manual V5193A Version 1 0 44 Controlling the detector Load Events 1 2 Signal 1 Signal 2 Prog off on 1 au au Prog lt Link 4 Hold 0 Time A1 A2 Run 1 min nm nm Load Events 1 2 Signal 1 Signal 2 Prog off0 on 1 au au Prog lt Link lt Hold 0 Time A1 A2 Run 1 min nm nm Load Events 2 Signal 1 Signal 2 Prog off on 1 au au Prog 4 P01 01 1 2 1 2 Setup Link 000 0 240 400 GLP Hold 0 Time At A2 Run 1 min nm nm Y Load Events 2 Signal 1 Signal 2 Prog off 0 on 1 au au Prog 4 P01 new time Setup Link4 9 000 0 GLP Hold 0 Time A1 2 Run 1 min nm nm Load Events 2 Signal 1 Signal 2 Prog offO on 1 au au Prog lt P01 01 1 2 1 2 Setup Link 000 0 240 400 j gt GLP Hold 0 Time A1 A2 Run 1 min nm nm Fig 12 Creating a program R
63. t Prerequisite Procedure KNAUER Connecting the detector to other devices WAN an additional patch cable is required which is also not supplied within the scope of delivery ssp eae ea at Seo LAN E RS ESI RE E RE RE RE Internet Fig 8 Cabling system LAN Computer is on There is a patch cable for each device and the computer 1 Use the patch cable to connect the router and the computer Repeat this step to connect all devices 2 Use the power supply to connect the router to the mains power system Configuring the Router The router is preset at the factory You can find a label at the bottom side of the router on which IP address user name and passwort are printed These information help to open the router configuration 1 To open the router configuration start your Internet browser and enter the IP address not for all routers 2 Enter user name and password Configure the router as DHCP server A w In the router configuration check the IP address range and make changes if necessary Once the router has assigned IP addresses to all devices the chromatography software can be used to remotely control the system Integrating the LAN into a Company Network A network administrator can integrate the LAN into a company network In this case the WAN port of the router is used There is a patch cable for the connection 1 Check that the IP address range of
64. the requirements of the recording device Specification of the range over which the output signal extends Maxi mum permissible is 5 au V If int sig or int ref is selected as data source the scaling unit is 1 V KNAUER Detector 2550 User Manual V5193A Version 1 0 38 Controlling the detector KNAUER ANALOG OUT OFFSET ANALOG IN Constant offset of the base line in both analog outputs Specification of a positive value 0 5 V for an offset in mV possible This value is added to the corresponding analog output signals Particularly useful when using chart records if the recordings of both channels should be separated or inverse chromatography is to be realized Any negative signals must be made view able with an offset as the analog outputs cannot provide a negative voltage Option for calibrating the analog input of the terminal strip for an external wave length control Using the vertical arrow keys in the set zero field it is possible define an applied input voltage as spectral zero point with a wavelength of 000 nm If a different positive control voltage is applied the current A field displays a resulting wavelength with scaling of 100 nm V This wavelength can then be adjusted finely by using the vertical arrow keys Thus a second calibra tion point is generated which modifies the scaling Note With respect to the maximum linearity it is recommended to scale the input voltage to 100 nm V The lon
65. the router and of the com pany network do not overlap 2 In case of an overlapping change the IP address range of the router 3 Use the patch cable to connect the router WAN port to the company network Detector 2550 User Manual V5193A Version 1 0 29 Note Procedure Result Note KNAUER Connecting the detector to other devices 4 Restart all device including the computer Controlling Several Systems Separately in a LAN Devices connected to a LAN communicate through ports which are part of the IP address If more than one HPLC system is con nected to the same LAN and you plan on controlling them sepa rately you can use different ports to avoid interference Therefore the port number for each device must be changed and this same number must be entered into the device configu ration of the chromatography software We recommend to use the same port number for all devices in the same system The port ist set to 10001 at the factory The IP port numbers in the device configuration of the chromatography software must be identical to those in the device otherwise the connection fails 1 Change the port number of the device 2 Enter the port number in the chromatography software The connection is established Refer to the troubleshooting chapter if you experience connec tion problems Troubleshooting for Connection Problems In case no connection between the computer and the devices can be establish
66. uents and other oper ating materials Storage KNAUER Disposal Hand in old devices or disassembled old components at a certi fied waste facility where they will be disposed of properly According to the German Abfallverzeichnisverordnung AVV January 2001 old devices manufactured by KNAUER are marked as waste electrical and electronic equipment 160214 KNAUER as a company is registered by the WEEE number DE 34642789 in the German Elektroaltger teregister EAR The number belongs to category 8 which among others comprises laboratory equipment All distributors and importers are responsible for the disposal of old devices as defined by the WEEE directive End users can send their old devices manufactured by KNAUER back to the dis tributor the importer or the company free of charge but would be charged for the disposal All eluents and other operating materials must be collected sep arately and disposed of properly All wetted components of a device e g flow cells of detectors or pump heads and pressure sensors for pumps have to be flushed with isopropanol first and water afterwards before being maintained disassembled or disposed The device can be stored within the following ambient condi tions Humidity below 90 96 noncondensing Temperature range 4 40 C 39 2 104 F Detector 2550 User Manual V5193A Version 1 0 59 Troubleshooting Troubleshooting KNAUER System messages
67. unning a program Before a program is run it must be loaded Procedure 1 Select the Program menu 2 Select the program number 3 Press horizontal arrow key to go to the editing mode of the submenu 4 Press AUTO ZERO key to go to ON OFF mode 5 Enter the program number and confirm with the horizontal arrow key The display switches to HOLD mode 6 Place the cursor onto HOLD and press 1 The display switches to RUN mode program is running KNAUER Detector 2550 User Manual V5193A Version 1 0 45 Controlling the detector 7 After the program has been completed press AUTO ZERO The display switches to ON OFF mode Load Events 1 2 Signal 1 Signal 2 Prog off 0 on 1 au au Prog Open P01 p Setup Link lt GLP Hold 0 Time Aq A2 Run 1 min nm nm Load Events 2 Signal 1 Signal 2 Prog off 0 on 1 au au Prog P01 00 2030 0109 Setup Link 000 00 240 400 GLP Hold 0 Time A1 A2 Run 1 min nm nm Load Events 1 2 Signal 1 Signal 2 Prog off 0 on 1 au au Prog P01 00 2030 0109 Setup Link HOLD 000 00 240 400 jJ GLP Hold 0 Time A1 A2 Run 1 min nm nm Load Events 2 Signal 1 Signal 2 Prog off on 1 au au Prog 4 P01 00 2030 0109 Setup Link RUN 019 00 240 400 jJ GLP Hold 0 Time A1 A2 Run 1 min nm nm Load Events _ _2 Signal 1 Signal 2 Prog off on 1 au au Prog P01 00 2030 0109 S
68. used in a local area network LAN the system status of the detector can be checked using the chromatography soft ware The detector is set to DHCP Dynamic Host Configuration Proto col at the factory This means that the detector is automatically assigned an IP address within the local network In the Setup menu this setting can be modified manually Operating range The detector can be used in analytical and preparative HPLC sys tems It is used in laboratories to analyze substance mixtures In an HPLC system the detector serves to detect substances in liq uids and show their concentration The device can be used in the following areas Biochemistry analysis Detector 2550 User Manual V5193A Version 1 0 Options Note KNAUER Intended Use Chemical analysis Food analysis Pharmaceutical analysis Environmental analysis The detector is e g used at universities research institutions and routine laboratories Where is use of the device prohibited DANGER Explosion hazard if the device is used in potentially explosive atmospheres without appropriate protective equipment Let specialists carry out protective measures Features Signal linearity and wavelength accuracy are verified by inde pendent measurements as part of a self calibration Within a system it is possible to measure four wavelengths simultaneously with the help of the software control Recording of a spectrum of 100 nm width in l
69. work days Also inform the freight carrier about transport damage Remove fastening material The device is held in place and protected by foam inserts at the top and bottom Please keep the transport box and the foam inserts Remove the foam insert on the top of the device e Remove the transport securing device Grip the device at its sides near the front and lift it out of the packaging Protective film on the display During transport a protective film prevents scratches to the screen of the device Detector 2550 User Manual V5193A Version 1 0 15 Scope of supply Scope of supply Use original parts and original accessories European hotline E mail KNAUER The delivery consists of Smartline detector 2550 with test cell Accessories Device manual Cables Power cable RS 232 port cable 2flat ribbon cables 10 pin LAN network cable Ethernet patch CAT5e 2 audio video connection cables 2 m 12 pin remote connector strip Stylus Transport securing device To maintain best performance only use original parts and accessories made by KNAUER or a company authorized by KNAUER Checking the scope of supply 1 Check that the device and accessories are complete 2 If anything is missing consult with KNAUER Technical Sup port Languages German and English Available by telephone 8 am to 5 pm CET Phone 49 0 30 809727 111 Fax 49 0 30 801 5010 E mail support knauer n
70. ymbols and Signs Symbols and Signs The following symbols and signs can be found on the device in the chromatography software or in the user manual Electric shock hazard Electrostatic discharge hazard damages to system device or components can occur General warning sign moderate injuries can occur and also damages to system device or components UV light hazard eye injuries can occur Hazardous substances A device or system marked with CE fulfills the product specific requirements of Euro pean directives This is confirmed in a Declaration of Conformity KNAUER Detector 2550 User Manual V5193A Version 1 0 13 Unpacking and Setup Unpacking and Setup Phone Fax E mail Requirements KNAUER Contacting the Technical Support You have various options to contact the Technical Support 49 30 809727 111 49 30 8015010 support knauer net You can make your requests in English and German Location Requirements The location for the device must meet the following require ments level surface for device or system Protect from heavy ventilation Weight 6 kg Dimension 226 x 135 x 410 mm Width x Height x Depth Power supply 100 240 V 50 60 Hz 75 W Humidity below 90 96 non condensing Temperature range 4 40 C 39 2 104 F Ventilation CAUTION Defect of the device due to overheating possible Protect the device against exposure to direct sun light Ma
71. ytical flow eells s 24 4 e7 5124 ER Gee Sft hae en B eO S Rete qan 66 Preparative flow cells 44 ueccbe eset VR EIER LEA T ees 67 LEZ View flow cells 2 mec ety en ka kp ed beo Ee PR durs Eu 68 Flow cell replacement parts ec cee ee eee RII 68 Test Cells sten d vore d EI UNE REUS TRB PME RAO RE EP UE 69 Legal Information xus do REN ERS UR RUP YOUR ER ATOR EX US 70 Warranty Conditions 2 s ses vi p X weet eae SOROR RON Ake ee s e Rn ns 70 Transportation Damages Sce uced ove Or ro EROR EIS CERRO RC E Rec ca 70 Abbreviations and terminology 0 e eee e eee ee eens 71 Declaration of Conformity cee cece eee ee ee eee 72 Table of EIGUEOS ces does as Ra SUP Sab RON AR MER Von Pw eR 73 I de C 74 To whom it may concern In case you prefer a French language user manual for this prod uct submit your request including the corresponding serial number via email or fax to KNAUER supportGknauer net 449 30 8015010 Thank you A qui que ce soit Si jamais vous pr f riez un manuel en francais pour ce poduit contacter KNAUER par email ou par fax avec le no de s rie supportGknauer net 49 30 8015010 Merci beaucoup KNAUER Detector 2550 User Manual V5193A Version 1 0 6 Intended Use HPLC Smartline UV detector 2550 Checking intended use Remote control Automatic configuration Device status LAN setting KNAUER Intended Use High

Smartline UV/VIS Detector 2550 user manual

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