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Agilent 1260 Infinity Preparative Pump

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1. The module should be operated in a horizontal position For a complete system including multiple pumps it is recommended to position the modules in two or more stacks Environment Your module will work within specifications at ambient temperatures and relative humidity as described in Table 4 on page 27 CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 26 Agilent 1260 Infinity Preparative Pump User Manual Site Requirements and Specifications 2 Physical Specifications Table 4 Physical Specifications Type Specification Comments Weight 15 0 kg 34 Ibs Dimensions 200 x 345 x 440 mm 8 x 13 5 x 18 width x depth x height Line voltage Line frequency Power consumption Ambient operating temperature Ambient non operating temperature Humidity Operating Altitude Non operating altitude Safety standards IEC CSA UL inches 100 240 VAC 10 50 or 60 Hz 5 250 VA 210 W 717 BTU 4 40 C 41 104 F 40 70 C 4 158 F lt 95 at 25 40 C 77 104 F Up to 2000 m 6500 ft Up to 4600 m 14950 ft Installation Category II Pollution Degree 2 Wide ranging
2. 181 Index pressure test 71 pressure 28 priming solvents 50 priming 49 pump head assembly 138 pump head ID module 151 pump head assembly 114 126 129 pump off while analysis 87 pump installing 29 unpacking 28 purge valve 114 radio interference 174 reassembling pump head assembly 129 pump head assembly 114 recommended pH range 28 REMOTE connector 39 remote timeout 76 remote interface 19 160 signal distribution 161 removing pump head assembly 126 pump head assembly 114 repair procedures 114 repairing inlet valve 114 outlet valve 114 valves 114 repairs replacing firmware 132 results pressure test 97 RS 232 connector 39 RS 232C connection table 162 RS 232C interface 162 182 RS 232 interface 18 runmode 73 running the leak test 99 running the pressure test 96 S safety class 168 safety information lithium batteries 173 safety general information 168 168 standards 27 symbols 171 schematics preparative pump 9 seal wash current low 91 seal wash function 66 seal wash pump cartridge 125 seal wash pump cassette 114 seal wash pump drive 151 seal wash tubings 44 sealwash 66 seal wash function 52 sealwash 92 seals 52 114 126 security lever 37 setable flowrange 28 shipping containers 30 shut down 76 simple repair procedures 114 simple repairs 108 site requirements 24 snap fastener 43 46 solvent cabinet parts 140 solvent counter zero 91 solvent filters cleaning 116 so
3. 9 Main Board PPM G1361 66500 10 Fan assembly 3160 1016 11 Electromagnetic purge valve EMPV G1361 60000 12 Cassette seal wash pump 5042 8507 Stepper motor seal wash pump 5065 4409 13 Leak pan pump 5042 8590 134 Agilent 1260 Infinity Preparative Pump User Manual Parts and Materials for Maintenance Pump Housing and Main Assemblies Figure 30 Overview of Main Assemblies Front View Agilent 1260 Infinity Preparative Pump User Manual 135 8 Parts and Materials for Maintenance Table 15 Repair Parts Pump Housing and Main Assemblies Rear View Item Description Part Number 1 Power supply behind rear panel 0950 3907 2 Standoff remote connector 1251 7788 3 Board cover 5001 3772 4 Screw 0515 0910 Lock washer 2190 0409 136 Agilent 1260 Infinity Preparative Pump User Manual Parts and Materials for Maintenance 8 Pump Housing and Main Assemblies 000000000 Tits sleielelriel CA M D itt tee erar C EEE T Figure 31 Overview of Main Assemblies Rear View Agilent 1260 Infinity Preparative Pump User Manual 137 Parts and Materials for Maintenance Pump Head Assembly 138 Table 16 Pump Head Assembly Item Description Part Number 12 13 Complete assembly Screw M5 55 mm lg Pump head front front and rear head sould be replaced at the same time it necessary Valve assembly for inlet and outlet Adapter for outlet valve Special screw
4. Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 172 Agilent 1260 Infinity Preparative Pump User Manual Appendix 10 Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig h ndtering Udskiftning ma kun ske med batteri af samme fabri
5. Agilent Technologies Notices Agilent Technologies Inc 2006 2007 2011 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61361 90012 Edition 02 2011 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant reg ula tions Otherwise it is intended only for gen eral laboratory use i s682 2 The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate writ
6. Exchange the Multi Assembly 5 Defective main board Exchange the main board Pressure Sensor not Connected The pressure readings read by the pump ADC analog digital converter are missing The ADC reads the pressure readings from the pressure sensor every 1 ms If the readings are missing for longer than 10 seconds the error message is generated Probable cause Suggested actions 1 Pressure sensor not connected should be Ensure the pressure sensor connector is clean connected to P29 on the PPM board or connected and seated correctly connected to the seal wash cable connector P23 2 Defective pressure sensor Exchange multi assembly 3 Defective PPM board Exchange the PPM board Agilent 1260 Infinity Preparative Pump User Manual 83 6 Troubleshooting and Diagnostics Wrong Data from Pressure Sensor The pressure sensor delivers readings within the range of 5 to 25 bar Probable cause Suggested actions 1 Defective pressure sensor Exchange the pressure sensor 2 Defective PPM board Exchange the PPM board Motor Drive Temperature too High The temperature of the motor drive circuits is too high The processor continually monitors the temperature of the drive circuits on the PPM board If excessive current is being drawn for long periods the temperature of the circuits increase If the temperature exceeds the upper limit of 95 C the error message is generated Probable cause Suggested actions 1 High fr
7. Two status indicators are located on the front of the preparative pump The lower left one indicates the power supply status the upper right one indicates the instrument status Status indicator 4 i Ce Power supply indicator Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON The power switch stays pressed in and a green indicator lamp in the power switch is ON when the preparative pump is turned ON When the line power switch stands out and the green light is OFF the preparative pump is turned OFF 72 Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 Instrument Status Indicator The instrument status indicator indicates one of four possible instrument conditions When the status indicator is OFF and power switch light is ON the module is in aprerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is ina not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a setpoint or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem whic
8. gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 1 Place the module on the bench in a horizontal position 2 Ensure the power switch on the front of the preparative pump is OFF switch stands out 3 At the rear of the preparative pump move the security lever to its maximum right position Status lamp L ea e Power switch Serial number Figure 7 Front View of the Preparative Pump Agilent 1260 Infinity Preparative Pump User Manual 37 3 Installing the Pump Installing the Preparative Pump oc o amp 9 LI C Cam CEE em e e epee a cS Power connector Figure 8 Power Connector and Safety Lever at Rear of the Preparative Pump 4 Connect the power cable to the power connector at the rear of the module see Figure 8 on page 38 5 If required connect additional interface and control cables to the preparative pump Figure 9 on page 38 See also Connecting Modules and Control Software on page 40 CAN cable to previous module Interface BCD board slot 8 bit configu ration switch 0000000 Remote CAN DC out RS232C CAN bus Figure 9 Preparative Pump Electrical Connections 38 Agilent 1260 Infinity Preparative Pump User Manual Installing the Pump 3 The CAN bus is a serial bus with high speed data tr
9. operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards 168 Agilent 1260 Infinity Preparative Pump User Manual Appendix 10 Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation M
10. unstable pressure Seal wash pump not delivering flow Seal wash pump leaking Leaks unstable flow or pressure After pumphead repair External internal leak Pressure ripple unstable run leak test for verification After replacing pump seals contaminated filter After replacing filter cup particles on filter frit If mechanically defect If seal life time is shorter than normally expected check plungers for scratches while changing the seals 114 Agilent 1260 Infinity Preparative Pump User Manual Maintenance 7 Checking and Cleaning the Solvent Inlet Filters A blocked filter does not affect the pressure readings of the preparative pump The pressure readings cannot be used to check whether the filter is blocked or not If the filter is in good condition the solvent will freely run out of the solvent tube due to hydrostatic pressure If the solvent filter is partly blocked only little solvent will drip out of the solvent tube When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used CAUTION Small particles can permanently block the capillaries and valves of the
11. 103 Prep Pump EMPV Cleaning Description 103 Running the Cleaning Procedure 104 aa Agilent Technologies 69 Troubleshooting and Diagnostics Agilent Lab Advisor software 70 The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1260 Infinity HPLC modules This includes tests and calibrations procedures as well as the different injector steps to perform all the maintenance routines A Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files This manual provides lists with the names of Not Ready messages and other common issues Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 Overview of the Pump s Indicators and Test Functions This chapter describes the instrument
12. 2 pm 5022 2192 1 CAN cable 0 5 m 5181 1516 1 Agilent 1260 Infinity Preparative Pump User Manual 33 3 Installing the Pump Optimizing the Stack Configuration If your preparative pump is part of a complete Agilent 1260 Infinity system you can ensure optimum performance and minimum delay volume by installing the following configuration e Figure 5 on page 34 and Figure 6 on page 35 show the configuration recommended for the pump in a UV based system Preparative System Solvent compartment Preparative pump A Column organizer Preparative pump B 7 UV vis detector Autosampler Fraction collector Figure 5 Recommended Stack Configuration Preparative System Front View 34 Agilent 1260 Infinity Preparative Pump User Manual NOTE Installing the Pump 3 Optimizing the Stack Configuration For information about connecting an LC MSD or a None 1260 Infinity detector to the system please refer to the User s Guide for Purification High Throughput System PN G2262 90010 or to the Installation Note for the Mass Based Fraction Collection Kit Se im Solvent Compartment mw Preparative Pump A Column Organizer GPIB or LAN 4 C to ChemStation a Preparative oo neo Pump B CAN Bus UVVis oo H ae Cable Detector xe C a d m Autosampler Fraction gg Collector NOTE Agilent 1260 Infinity Preparative Pump User Manual Figure 6 Recommended Stack Configuratio
13. 3 Installing the Pump Connecting Modules and Control Software WARNING Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Connecting Agilent 1260 Infinity modules 1 Place the individual modules in a stack configuration as shown in Figure 5 on page 34 2 Ensure the power switches on the front of the modules are OFF switches stand out 3 Plug a CAN cable into the CAN connector at the rear of the respective module except vacuum degasser 4 Connect the CAN cable to the CAN connector of the next module see Figure 6 on page 35 5 Press in the power switches to turn on the modules 40 Agilent 1260 Infinity Preparative Pump User Manual Installing the Pump 3 Connecting control software control module 1 Ensure the power switches on the front of the modules in the stack are OFF switches stand out 2 Plug a GPIB cable into the GPIB connector at one of the modules preferably at the detector MUST for the DAD 3 Connect the GPIB cable to the Agilent control software in use 4 Plug a CAN cable into the CAN connector of the control module Do not connect the Agilent control software or the control module with the vacuum degasser 5 Connect the CAN cable to the CAN connecto
14. Preparative Pump 8 Hydraulic Path Overview 9 How Does Compressibility Compensation Work 10 Early Maintenance Feedback EMF 11 Instrument Layout 12 The Electronics 13 Electrical Connections 14 Interfaces 16 ee Agilent Technologies 1 Introduction to the Preparative Pump Introduction to the Preparative Pump Introduction to the Preparative Pump The preparative pump is a cam driven pump with fixed stroke and two parallel pistons each with identical ball and seat inlet and outlet valve Each of the two pump pistons is capable of delivering a maximum of 50 ml min flow at up to 400 bar pressure The two cams have overlapping delivery cycles Compression losses are calculated and smooth delivery is ensured through variation of the motor speed over the pump cycle The pistons are guided through two seals each The gap between those seals is connected to capillary fittings this way seal wash is not only an option but integral part of every pump A peristaltic pump to automate seal wash is also part of the system Pump drive Power supply PPM board Fittings seal wash Fan Outlet valves Pump head Inlet valves Electromagnetic purge valve Leak eak sensor Seal wash pump Multi Assembly with filters and pressure sensor at the rear Figure 1 Overview of the Preparative Pump 8 Agilent 1260 Infinity Preparative Pump User Manual Introduction to the Preparative Pump 1 H
15. Pump The parameters for setting up the preparative pump can be accessed through the instrument menu or by clicking on the pump icon in the system diagram see Figure 15 on page 58 Figure 15 System Diagram The Setup dialog box depends on the configuration of the pump In Figure 16 on page 58 you see the dialog box for the isocratic pump and in Figure 17 on page 59 you see the interface for the gradient pump Set up Pump 1 Instrument 1 m Control x Pressure Limits Purge C enabled disabled purgetime 0 00 min Flow 50 00 ml min Max 400 bar StopTime 360 00 min F in PostTime Off min fo bar gt Solvent A 100 0 H20 65 ACN 35 m Timetable Time Flow Max Press Insert Cut im Copy Paste Display Timetable OK Figure 16 Cancel Help Setup dialog box isocratic pump Agilent 1260 Infinity Preparative Pump User Manual Using the Preparative Pump 4 x r Control Pressure Limits Purge Solvent Flow EMRE mimin Max C enabled disabled 400 bar StopTime 30 00 fmin i purgetime 0 00 min in P ae Note Don t forget to set up PostT ime Off min fo bar purging for solvent B r Solvent A Solvent B 800 Water J20 facetonitle m Timetable Insert Append Eut Copy Faste Display Timetable Cancel Help Figure 17 Setup
16. actions 1 Value for bottle filling set incorrectly Reset bottle filling to the correct value 2 Solvent bottle empty Refill solvent bottle No Motor Motion No motor rotation feedback Probable cause Suggested actions 1 Defective or disconnected index cable Ensure the connector connected and seated correctly 2 Encoder cable not connected at the encoder Ensure the encoder cable is connected and seated correctly at the encoder 3 Motor defective or not connected Exchange the pump drive assembly 4 Defective main board Exchange the main board Seal Wash Current Low The current sensing of the seal wash motor was lower than expected Probable cause Suggested actions 1 Defective or disconnected seal wash pump Ensure the cable is correctly connected motor Exchange the seal wash pump motor 2 Defective main board Exchange main board Agilent 1260 Infinity Preparative Pump User Manual 91 6 Troubleshooting and Diagnostics Seal Wash Safety CAUTION Pressure sensor incorrectly plugged into the main board port You risk permanent damage of the pressure sensor if the seal wash pump is turned ON while the pressure sensor is incorrectly connected to the main board port dedicated for the seal wash pump This requires exchange of complete multi assembly gt Never turn on the seal wash pump while the pressure sensor is connected to the main board port gt Make sure the seal wash cable and the pressure senso
17. capability Maximum Non condensing For storing the module For indoor use only Agilent 1260 Infinity Preparative Pump User Manual 27 2 Site Requirements and Specifications Performance Specifications 28 Table5 Performance Specification Agilent 1260 Infinity Preparative Pump Type Hydraulic system flow rangeSettable Flow precision Pressure range Compressibility compensation Recommended pH range Control and data evaluation Communications Safety and maintenance GLP features Housing Specification Dual pistons in parallel 0 001 100 ml min lt 0 5 RSD 20 to 400 bar 5880 psi system pressure User selectable based on mobile phase compressibility 1 0 12 5 solvents with pH lt 2 3 should not contain acids which attack stainless steel Agilent ChemStation for LC and LC MS Controller area network CAN RS 232 APG Remote ready start stop and shut down signals CAN DC OUT LAN optional Extensive diagnostics error detection and display through Agilent ChemStation leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas Early maintenance feedback EMF for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with user settable limits and feedback messages Electronic records of maintenance and errors All materials recyclable Agilent 1260 In
18. dialog box gradient pump In these dialog boxes you can specify the following parameters Flow Flow rate delivered by the pump For gradient systems the flow parameter defines the combined flow delivered by the system on channel A and B The maximum settable flow is 100 ml min For best performance a flow above 5 ml min should be chosen The pump can be operated at lower flow rates However a slightly higher pressure ripple will be observed In addition the system pressure should always be kept above 20 bar Stop Time The Stop Time defines the end of your analysis run After the stop time all gradients are stopped and the pump settings return to the initial values Typically the Stop Time of the pump specifies the Stop Time for all other modules in the LC system Post Time During the Post Time the instrument stays in the Not Ready state and delays the start of the next analysis The Post Time allows the column to equilibrate after a gradient run Agilent 1260 Infinity Preparative Pump User Manual 59 4 Using the Preparative Pump Max Pressure The pump shuts down when exceeding the maximum pressure limit to protect the system or the column against overpressure The Preparative Pump can operate up to 400 bar over the entire flow range The limit has to be changed if columns or other components with lower pressure limits are part of the LC system Min Pressure The pump shuts down if the pressure is below this limit
19. direct sunlight e Filter HPLC grade dry Acetonitrile before use The dryer the Acetornitrile the stronger the tendency to form polymers and therefore block the system Refilter at least every two days Never use the system without solvent filter installed 54 Agilent 1260 Infinity Preparative Pump User Manual Using the Preparative Pump 4 Algae Growth in HPLC Systems The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems Algae grow in aqueous media preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light will also stimulate their growth Even in distilled water small sized algae grow after some time Instrumental Problems Associated With Algae Algae deposit and grow everywhere within the HPLC system causing e Deposits on ball valves inlet or outlet resulting in unstable flow or total failure of the pump e Small pore solvent inlet filters to plug resulting in unstable flow or total failure of the pump e Small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure e Column filters to plug giving high system pressure Flow cell windows of detectors to become dirty resulting in higher noise levels since the detector is the last module in the flow path this problem is less co
20. for several seconds Purge Solvent A If the purge function is enabled the purge valve EMPV will open for the specified time before each analysis In this dialog box you can only control the automated purge for pump A If you are using a gradient system choose Purge B to specify and enable the purge procedure on channel B see Figure 18 on page 60 Purge B Instrument 1 x m Purge Solvent B C enabled disabled purgetime 0 00 min Cancel Help Figure 18 Purge Dialog Box Solvent A This text field allows you to write down a brief description of solvent A Solvent B This text field allows you to write down a brief description of solvent B on gradient systems In addition you can specify the initial proportion of solvent B in This composition is valid as soon as the pump is switched on before the run and after the Stop Time During the run the composition gradient can be specified with the timetable Timetable With the timetable you can specify changes of the composition B the flow and the maximum pressure limit during the run It is used to define solvent gradients The composition B is only available on gradient systems Agilent 1260 Infinity Preparative Pump User Manual Using the Preparative Pump 4 Flow Ramp and Compressibility can be specified in the Auxiliary dialog box see Figure 19 on page 61 Flow Ramp This is the rate for turning the flow up and down when the pump is
21. interface boards for the Agilent 1260 Infinity Series are BCD Board e LAN Communication Card Agilent 1260 Infinity Preparative Pump User Manual 13 1 Introduction to the Preparative Pump Electrical Connections 14 The GPIB connector is used to connect the module with a computer The address and control switch module next to the GPIB connector determines the GPIB address of your module The switches are preset to a default address and is recognized once after power is switched ON The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal Agilent 1260 Infinity module data transfer and synchronization One analog output provides signals for integrators or data handling systems The interface board slot is used for external contacts and BCD bottle number output or LAN connections The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch See your software documentation for further information The power input socket accepts a line voltage of 100 240 volts AC 10 with a line frequency of 50 or 60 Hz Maximum power con
22. is delivered with your pump The seal wash function cannot be operated permanently danger of overheating Switch on the seal wash pump in regular intervals normally for 30 s every 30 minutes for a certain length of time It is however important to keep the back of the seals wet with the help of the seal wash function Water or Aqueous Buffer Solutions We recommend a composition of 10 Isopropanol in HPLC grade water for water or aqueous buffer solutions With buffer solutions the seal wash pump should be configured such that it is turned on for a duration of 30 s every 5 minutes with a maximum of 30 cycle time Shorten the intervals between the pump turn ons for highly concentrated buffer solutions gt 0 1 M buffer solutions Organic Solvents For all organic non buffered solutions we recommend to use the same solvent for the seal wash as the one that is currently pumped by your preparative pump In this case the seal wash pump has to be operated in large intervals only Basically it is enough to keep the back of seals wet with the help of the seal wash option Beware that the seal wash tubing is made of silicon Never use any solvents for the seal wash that are not compatible with silicone 66 Agilent 1260 Infinity Preparative Pump User Manual Optimizing Performance 5 How to Optimize the Compressibility Compensation Setting The compressibility compensation default settings are 75 x 10 bar best for most aqueous
23. main power switch unplug the pump from line power Disconnect cables from the interfaceboard connectors Loosen the screws Slide out the interface board from the pump Install the new interface board Secure screws oF W N Reconnect the cables to the board connector BCD Interface board Figure 29 Exchanging the Interface Board Agilent 1260 Infinity Preparative Pump User Manual 131 7 Maintenance Replacing the Module s Firmware When Tools required Parts required Preparations 132 The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third part control software requires a special version LAN RS 232 Firmware Update Tool or Agilent Diagnostic Software Instant Pilot G4208A only if supported by module Description Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware the following steps have to be performed 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 Load
24. module Damage of the module gt Always filter solvents gt Never use the module without solvent inlet filter Agilent 1260 Infinity Preparative Pump User Manual 115 7 Maintenance Cleaning the Solvent Filters When If solvent filter is blocked Parts required Description Concentrated nitric acid 65 Bidistilled water 1 Beaker Preparations Remove the solvent inlet tube from the inlet valve of the pump and let the tube hang down 1 Remove the blocked solvent filter from the bottle head assembly and place it in a beaker with concentrated nitric acid 65 for one hour 2 Thoroughly flush the filter with bidistilled water remove all nitric acid some columns can be damaged by nitric acid 3 Replace the filter 116 Agilent 1260 Infinity Preparative Pump User Manual Maintenance 7 Exchanging the Inlet Outlet Ball Valves When Tools required Parts required Valve assembly Valve adapter Removing an Inlet Ball Valve Bottom of the Pump Head If internally externally leaking back flow Wrench 17 mm Wrench 1 4 inch Part number Description 1 G1361 60012 Valve assembly The inlet valves do not necessarily have to be replaced in pairs Use the built in Leak Test see Prep Pump Leak Test Description on page 98 to identify the valve that is not working properly 1 Disconnect the solvent inlet tube at the inlet valve Beware of leaking solvents 2 Using a 17 mm wrench loosen the inle
25. on and an entry is written into the instrument logbook Timeout The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required Agilent 1260 Infinity Preparative Pump User Manual 75 76 Troubleshooting and Diagnostics Shut Down An external instrument has generated a shut down signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system Remote Timeout Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition A not ready condition is
26. pressure test from the test selection menu 2 Start the test and follow the instructions Finishing the test will release the pressure in the system 3 Remove the blanking nut after finishing the test and reconnect all capillaries For detailed instructions refer to the Agilent LMD Tool 96 Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 Evaluating the Results The sum of all leaks between the pump and the blank nut will be indicated by flow rate of gt 10 ul minute to keep the pressure constant Note that small leaks may cause the test to fail but solvent may not be seen leaking from a module Please notice the difference between an error in the test and a failure of the test An error means that during the operation of the test there was an abnormal termination If a test failed this means that the results of the test where not within the specified limits Do not overtighten fittings After the first major increase of resistance when tightening a previously loose fitting the fitting should not be tightened any further than an additional 1 8 to 1 4 wrench turn Often it is only a damaged blank nut itself poorly shaped from overtightening that causes a failure of the test Before investigating on any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened Large internal leaks at the EMPV can easily be identified by observin
27. s built in troubleshooting and test functions Status Indicators The preparative pump is provided with two status indicators which indicate the operational state prerun run and error states of the preparative pump The lower left indicates the power supply status the upper right indicates the preparative pump status The status indicators provide a quick visual check of the operation of the preparative pump see Status Indicators on page 72 Error Messages In the event of an electronic mechanical or hydraulic failure the preparative pump generates an error message in the user interface For details on error messages and error handling please refer to the Agilent Lab Monitor amp Diagnostic Software Pressure Test The pressure test is a quick test designed to determine the pressure tightness of the system After exchanging flow path components e g pump seals or injection seal use this test to verify the system is pressure tight up to 400 bar see Prep Pump Pressure Test Description on page 94 Leak Test The leak test is a diagnostic test designed to determine the pressure tightness of the preparative pump When a problem with the preparative pump is suspected use this test to help troubleshoot the preparative pump and its pumping performance see Prep Pump Leak Test Description on page 98 Agilent 1260 Infinity Preparative Pump User Manual 71 6 Troubleshooting and Diagnostics Status Indicators
28. still present on the remote input When an analysis is started the system expects all not ready conditions e g a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line 2 Defective remote cable 3 Defective components in the instrument showing the not ready condition Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s reference documentation Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 Synchronization Lost During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board i
29. switched on or off The default setting 800 ml min min protects the column against sudden pressure peaks and drops Compressibility The compressibility of the solvent has to be specified in this field The value will be used for the compressibility compensation to optimize the performance of the pump For more details read the chapter How to Optimize the Compressibility Compensation Setting on page 67 Auxiliary A Instrument 1 DE10200112 x m Flow Ramp o ml min per minute r Compressibility 75 10 soar Cancel Help Figure 19 Auxiliary Dialog Box The Auxiliary dialog box is also available for channel B on gradient pump systems The Control dialog box Figure 20 on page 62 allows you to switch the pump on an off Purge In this field you can open and close the automated purge valve or you can specify a time for how long the purge valve will be open Error Method By setting an Error Method you can define a pump particular method that is run if an instrument error is detected It is typically used to shut down the pump in a controlled manner Agilent 1260 Infinity Preparative Pump User Manual 61 4 62 Using the Preparative Pump Use the Setup Pump dialog box Figure 16 on page 58 or Figure 17 on page 59 to define a suitable error method Then select Take Current Method and click OK The current method is immediately copied to the pump and stored as error method and executed in ca
30. the firmware into the module as described in the documentation Due to a different hardware platform there is no way to convert a G1314D VWD and the G1314E VWD SL Plus to a G1314A B VWD or G1314C VWD SL Agilent 1260 Infinity Preparative Pump User Manual Agilent 1260 Infinity Preparative Pump User Manual 8 Parts and Materials for Maintenance Pump Housing and Main Assemblies 134 Pump Head Assembly 138 Solvent Cabinet and Solvent Inlet Parts 140 Hydraulic Path 142 Preparative Pump Basis Kit 145 Preparative Pump Gradient Kit 147 ee Agilent Technologies 133 8 Parts and Materials for Maintenance Pump Housing and Main Assemblies Table 14 Repair Parts Pump Housing and Main Assemblies Front View Item Description Part Number 1 Leak sensor 5061 3356 2 Multi assembly with filters and pressure sensor at the rear G1361 60005 Filter cup 10 um 3150 0942 SST filter assembly 2 pm 5022 2192 3 Inlet valve see Removing an Inlet Ball Valve Bottom of the Pump Head on page 117 4 Pump head see Removing and Disassembling the Pump Head G1361 60004 Assembly on page 126 5 Outlet valve see Removing an Outlet Ball Valve Top of the Pump Head on page 118 6 Cap seal wash see Removing and Disassembling the Pump Head Assembly on page 126 7 Pump drive assembly G1361 60001 8 Power supply underneath the PPM board main assembly only 0950 3907 for additional parts refer to the Repair Manual
31. the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself Agilent 1260 Infinity Preparative Pump User Manual Introduction to the Preparative Pump 1 The Electronics The electronics are comprised of four main components e The preparative pump main board PPM see Preparative Pump Main Board PPM on page 150 e Power supply see Main Power Supply Assembly on page 164 Optional e Interface board BCD external contacts see BCD External Contact Board on page 155 e LAN Communication Card see LAN Communication Interface Board on page 157 Main Board The board controls all information and activities of all assemblies within the module The operator enters parameters changes modes and controls the module through interfaces CAN GPIB or RS 232C connected to the user interfaces Main Power Supply Assembly The main power supply comprises a closed assembly no on site repair possibility The power supply provides all DC voltages used in the module except for the voltages supplied by the lamp power supply to the deuterium and tungsten lamps in the detectors The line voltage can vary in a range from 100 240 volts AC 10 and needs no manual setting Optional Interface Boards The Agilent 1260 Infinity modules have one optional board slot that allows to add an interface board to the modules Optional
32. the module Place the bottles into the solvent cabinet and place a bottle head assembly into each bottle Ground the solvent bottles 4 Connect the solvent tubes from the bottle head assemblies to the inlet adapters of the pumps Fix the tubes in the clips of solvent cabinet and preparative pumps 5 By holding a piece of sanding paper around the waste tubing connect it to the electromagnetic purge valve EMPV of each pump and place it into your waste system 6 If one of the preparative pumps is placed on the bottom of a stack connect the corrugated waste tube to the waste outlet of the pump leak handling system In order to guarantee an error free handling of any leakage that may occur in the system all modules of a stack must be thoroughly aligned on top of each other Outstanding knobs at the rear of the top covers of the modules and holes as counterparts at the rear of the bottom covers of the modules will help to align the instruments nicely on top of each other by making them slide into the correct position This will then allow any possible spills to be routed safely from one instrument to the next one with the help of the built in leak drainage system Each module has a funnel to catch drops coming from the above instrument from there spills a lead through a waste drain tube into the leak pan and out from there through an outlet on to the next module Agilent 1260 Infinity Preparative Pump User Manual Installing the Pump 3
33. to damage seal surface Pump seals Pump head front 2 Take rear pump head and place on top of front pump head Beware that the O rings are in proper place and the seal wash fitting is facing in same direction as outlet valves Seal wash fitting Outlet ball valve Agilent 1260 Infinity Preparative Pump User Manual 129 7 Maintenance 3 Insert and tighten special screws using a 10 mm wrench Special screws 4 Insert new secondary seals with the spring facing the pump body Press the seals firmly into place Secondary seals 5 Place Spring assembly on special screws and tighten the two flathead screws using a pozidrive 1 The RF tag must be on the upper side of the pumphead Flathead screws Spring assembly RF tag 6 Reinsert Plungers by pushing until Plunger collars hit springs 130 Agilent 1260 Infinity Preparative Pump User Manual Maintenance 7 Exchanging the Optional Interface Board When Board defective Parts required Description 1 BCD Interface board see BCD External Contact Board on page 155 CAUTION Electrostatic discharge at electronic boards and components Electronic boards and components are sensitive to electrostatic discharge ESD gt 228 In order to prevent damage always use an ESD protection for example the ESD wrist strap from the accessory kit when handling electronic boards and components 1 Switch off the pump at the
34. 1260 Infinity Preparative Pump User Manual Installing the Pump 3 Flow Connections for a Dual Binary Gradient Preparative Pump Tools required Parts required Preparations Wrench 1 4 5 16 inch for capillary connections Part number Description Other modules 1 G1361 68708 Parts from basis kit see Table 8 on page 32 1 G1361 68707 Parts from gradient kit see Table 9 on page 33 Pumps are installed in the HPLC system When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Danger of Explosion Pumping solvents at high flow rates generally results in electrostatic charging of the solvents which may result in the potential ignition of flammable vapors or solvents gt To avoid electrostatic discharge never use the pump without grounded metal solvent bottles gt Do not use metal bottles without appropriate grounding Agilent 1260 Infinity Preparative Pump User Manual 45 3 46 Installing the Pump 1 Remove the front covers of both pumps by pressing the snap fasteners on both sides Figure 12 Removing the Front Cover 2 Place the solvent cabinet on top of
35. 176 flow connections gradient pump 45 isocratic pump 42 flow 42 frequency range 27 front cover 43 front view isocratic preparative pump 37 fuse 151 fuses on BCD board 155 G GPIB interface 159 GPIB interface 18 gradient kit 30 grounding of solvent bottle 43 grounding metal bottles 42 45 H hints for successful use 52 housing 28 HP JetDirect card 157 humidity 27 hydraulic path parts 142 hydraulic system 28 28 index board 151 index missing 87 indicator status 72 inlet valve 117 inlet valves 114 installation power cords 25 pump module 36 installing the pump 29 instrument status indicator 73 amp 73 interconnection Diagram Agilent 1260 Infinity Preparative Pump User Manual Index CSM board 154 interface board 39 131 interface Agilent 1260 Infinity 16 analog signal output 19 CAN 18 159 GPIB 18 159 remote 19 160 RS 232C 162 RS 232 18 interfaces 152 152 interface special 21 internet 178 isocratic preparative pump 37 L laboratory bench 26 lamp status 72 lamp instrument status 73 LAN communication interface board 157 eak sensor short 79 eak sensor open 78 eak test evaluation 100 eak test 98 eak converter 150 eaktest 71 eakage 43 eak 78 ine frequency 27 39 ine voltage 27 39 lithium batteries 173 logbook 75 M main assemblies overview 112 13 13 150 maintenance procedures 11 main board maintenance replacing firmware 132 Materials in conta
36. 67306 1 Spare bottle inlet filter frit 3150 0944 1 Spare frit adapter for 4 7 mm OD tubing G1361 23205 1 Glass stop valve for stopped pumps 5042 6464 1 Spare filter cup 3150 0942 1 Spare SST frit 2 um 5022 2192 1 CAN cable 0 5 m 5181 1516 1 Agilent 1260 Infinity Preparative Pump User Manual 147 8 Parts and Materials for Maintenance Preparative Pump Gradient Kit 148 Agilent 1260 Infinity Preparative Pump User Manual Agilent 1260 Infinity Preparative Pump User Manual 9 Configuring the Preparative Pump Preparative Pump Main Board PPM 150 Block Diagrams 152 Optional Interface Boards 155 Interfaces 159 Analog Signal Output 159 GPIB Interface 159 CAN Interface 159 APG Remote Interface 160 RS 232C 162 Main Power Supply Assembly 164 ee Agilent Technologies 149 9 Configuring the Preparative Pump Preparative Pump Main Board PPM 150 The board controls all information and activities of all assemblies within the preparative pump The operator enters parameters changes modes and controls the preparative pump through interfaces CAN or RS 232C connected to the user interfaces Figure 34 on page 152 and Figure 35 on page 153 show block diagrams of this board ASIC Application Specific Integrated Circuit The application specific integrated circuit ASIC includes all digital logic for the core processor functions and also for module specific functions Motor Drive The drive comprises motor control an
37. 7 Connect the long capillary 300 mm G1361 67306 to the electromagnetic purge valve EMPV of the upper pump and connect the female T piece to the other end of the capillary 8 Connect the medium length capillary 140 mm G1361 67304 to the electromagnetic purge valve EMPV of the lower pump and connect the other end of the capillary to the female T piece 9 Install the mixer 79835 87330 in the clips on the front panel of the bottom pump 10 Connect the capillary G1361 67305 from the outlet of the female T piece to the mixer 11 Install the outlet capillary G1361 67302 to the mixer and make the necessary connections the other side of the capillary normally a capillary to an injector or injection valve 12 Install the seal wash tubings as shown in Figure 13 on page 48 You need for each of the pumps a tubing from your seal wash solvent bottle to the seal wash pump one from there to the pump head and one from the pump head to waste 13 Install the front cover Agilent 1260 Infinity Preparative Pump User Manual 47 3 Installing the Pump 14 Press in the power switch to turn ON the preparative pumps From solvent bottle A Outlet ball valves Tube clip Leak funnel Tubing Leak drainage Seal wash to waste g EMPV out to bottom pump G1361 67306 Inlet ball valves From seal wash solvent bottle From solvent bottle B Female T piece Tubing Seal wash to
38. Agilent 1260 Infinity Preparative Pump User Manual Maintenance 7 Exchanging the Seal Wash Pump Cartridge When If mechanically defect Parts required Part number Description 1 5042 8507 Seal wash pump cartridge 1 Disconnect seal wash tubing at the connectors and place the ends of each tubing at a level above seal wash bottle to avoid leaking solvents 2 By pressing the upper and lower clip remove the seal wash pump cartridge 3 Replace the defective cassette and slide the new cassette on to the shaft pressing upper and lower clip Push forward until the clips snap into place The preferred orientation of the cartridge is as shown in picture below 4 Reconnect seal wash tubing Seal wash connectors Figure 28 Seal Wash Pump Cartridge Agilent 1260 Infinity Preparative Pump User Manual 125 7 Maintenance Removing and Disassembling the Pump Head Assembly When When leaky or blocked For exchanging the seals For exchanging the plungers Tools required Screwdriver Pozidrive 1 4 mm hexagonal key 10 mm wrench Toothpick Preparations Switch OFF preparative pump at power switch Remove the front cover to have access to the pump mechanics Seals should always be replaced as pairs 2x front seals 2x back seals CAUTION Damage of the pump drive Starting the pump when the pump head is removed may damage the pump drive gt Never start the pump when the pump head is removed 126 Agilen
39. Cable coon onoonFrwonr coon oonrFrwonr Oo w Agilent 1260 Infinity Preparative Pump User Manual DCD RX TX DTR GND DSR RTS CTS RI DB9 Configuring the Preparative Pump Interfaces 163 Configuring the Preparative Pump Main Power Supply Assembly 164 The main power supply comprises a closed assembly no on site repair possibility The power supply provides all DC voltages used in the module except for the voltages supplied by the lamp power supply to the deuterium and tungsten lamps in the detectors The line voltage can vary in a range from 100 240 volts AC 10 and needs no manual setting MPS 436V m 36V not used supply line i iai gt me supply voltage circuits 24V 4 5A 5V switching gt 5V 3A regulator linear voltage gt FIONDA regulators 15V 3A Figure 38 Main Power Supply MPS Blockdiagram No accessible hardware fuse is needed because the main power supply is safe against any short circuits or overload conditions on the output lines When overload conditions occur the power supply turns off all output voltages Turning the line power off and on again resets the power supply to normal operation if the cause of the overload condition has been removed Agilent 1260 Infinity Preparative Pump User Manual Configuring the Preparative Pump 9 An over temperature sensor in the main power supp
40. Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear of the module The male end of each power cord is different and designed to match the wall socket of a particular country or region The absence of ground connection and the use of an unspecified power cord can lead to electric shock or short circuit Electric Shock gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1260 Infinity Preparative Pump User Manual 25 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 4 on page 27 allow to place the module on almost any laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 3 1 inches in the rear for the circulation of air and electric connections If the bench should carry a complete Agilent 1260 Infinity system make sure that the bench is designed to carry the weight of all the modules
41. Infinity Preparative Pump User Manual Appendix 10 Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing strong complexing agents e g EDTA Mixtures of carbon tetrachloride with 2 propanol or THF Agilent 1260 Infinity Preparative Pump User Manual 177 10 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the Agilent 1260 Infinity modules for download 178 Agilent 1260 Infinity Preparative Pump User Manual Index Index A adapter 43 46 additional interface and control cables 38 Agilent Diagnostic software 70 Agilent Lab Advisor software 70 Agilent Lab Advisor 70 Agilent on internet 178 algae growth 54 algae 52 176 176 ambient operating temperature 27 ambient non operating temperature 27 analog signal output 19 APG remote connector 160 APG remote connector 19 aqueous buffer solutions 66 ASIC application specific integrated circuit 150 back pressure 52 basis kit parts 145 basis kit 32 basis kit 30 145 battery 151 safety information 173 BCD board external contacts 155 155 BCD board 39 131 bench space 26 blank nut 104 blanknut 96 99 bloc
42. M6 Cap seal wash fittings Screw for plunger housing Sapphire plunger Plunger housing Seal prep flange Pump head rear front and rear head sould be replaced at the same time it necessary O ring Adapter for inlet valve G1361 60004 0515 4249 G1361 25200 G1361 60012 G1361 23202 G1361 22401 G1361 67307 5015 0048 G1361 22402 G1361 60007 5022 2188 G1361 25201 0905 1516 G1361 23201 Agilent 1260 Infinity Preparative Pump User Manual Parts and Materials for Maintenance 8 Pump Head Assembly Figure 32 Pump Head Assembly Agilent 1260 Infinity Preparative Pump User Manual 139 8 Parts and Materials for Maintenance Solvent Cabinet and Solvent Inlet Parts Table 17 Solvent Cabinet and Bottle Head Assembly Parts Item Description Part Number 1 Solvent cabinet including all plastic parts 5065 9981 2 Name plate Agilent 1200 5042 8901 3 Front panel solvent cabinet 5065 9990 4 Leak pan solvent cabinet 5042 8907 5 Bottle head assembly for Prep pump includes items 6 7 8 9 G1361 60022 and 10 6 Y fitting and tube pump 7 Cap bottle 8 Bottle amber 2 liter 9301 6341 8 Bottle clear 2 liter 9301 6342 Bottle clear 2 liter with second inlet 5065 4421 9 Frit adapter G1361 23205 10 Solvent inlet filter 3150 0944 11 Prep Pump SST inlet adapter for pressurized solvent supply G1361 60008 Allows connection of any 1 8 Swagelock compatible capillary to the inlet of the prep pump 12 HiFlow S
43. Parts and Materials for Maintenance on page 133 There are two different kits that can possibly be delivered with the pump 1 A Basis kit 1361 68708 is delivered with every isocratic configuration or if the pump is the first pump in a system isocratic configuration order No G1361A 2 A Gradient kit G1361 68707 is delivered with every additional pump that is added to a system s first pump gradient configuration order No G1391A Please report missing or damaged parts to your local Agilent Technologies sales and service office Agilent 1260 Infinity Preparative Pump User Manual Installing the Pump 3 Table6 First Preparative Pump G1361A Delivery Checklist Description Quantity Preparative Pump 1 Corrugated waste tubing 1 Power cable local 1 Service Manual 1 Solvent cabinet 1 Basis kit see Table 8 on 1 with every FIRST G1361A pump of a system only page 32 Table 7 Gradient Preparative Pump G1391A Delivery Checklist Description Quantity Preparative Pump 1 Corrugated waste tubing 1 Power cable local 1 Gradient kit see Table 9 on 1 with very ADD ON G1391A pump of a system page 33 Agilent 1260 Infinity Preparative Pump User Manual 31 3 Installing the Pump Basic Kit Contents Table8 Basis Kit Contents G1361 68708 shipped with the first pump of a system Description Part Number Quantity Bottle head assembly G1361 60022 1 Tube seal wash 2 m 0890 1764 1 Waste tu
44. Performance 65 How to Configure the Seal Wash Function 66 How to Optimize the Compressibility Compensation Setting 67 6 Troubleshooting and Diagnostics 69 Agilent Lab Advisor software 70 Overview of the Pump s Indicators and Test Functions 71 Status Indicators 72 User Interfaces 74 Error Messages 75 Pressure Test 94 Leak Test 98 EMPV Cleaning 103 7 Maintenance 107 Introduction to Maintenance and Repair 108 Overview of Maintenance and Repair 112 Simple Repairs 114 8 Parts and Materials for Maintenance 133 Pump Housing and Main Assemblies 134 Pump Head Assembly 138 Solvent Cabinet and Solvent Inlet Parts 140 Hydraulic Path 142 Preparative Pump Basis Kit 145 Preparative Pump Gradient Kit 147 9 Configuring the Preparative Pump 149 Preparative Pump Main Board PPM 150 Optional Interface Boards 155 Interfaces 159 Main Power Supply Assembly 164 4 Agilent 1260 Infinity Preparative Pump User Manual Contents 10 Appendix 167 General Safety Information 168 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 172 Lithium Batteries Information 173 Radio Interference 174 Sound Emission 175 Solvent Information 176 Agilent Technologies on Internet 178 Agilent 1260 Infinity Preparative Pump User Manual Contents 6 Agilent 1260 Infinity Preparative Pump User Manual Agilent 1260 Infinity Preparative Pump User Manual 1 Introduction to the Preparative Pump Introduction to the
45. The side of the valve assembly that has the two holes must always face in flow direction 3 Insert the valve adapter with the new valve assembly into the housing and screw tight 4 Reconnect all capillaries and tubings Agilent 1260 Infinity Preparative Pump User Manual Maintenance 7 Simple Repairs NOTE Don t reverse inlet and outlet valve adapters The outlet valve adapters have a smaller hole for fitting of the SST outlet capillary The inlet adapters are designed for the finger tight fittings of the inlet tubing Agilent 1260 Infinity Preparative Pump User Manual 119 7 Maintenance Exchanging the Filter Cup When Tools required Parts required 120 After the pump seals have been exchanged When the back pressure exceeds 10 bar pumping 50 ml min of pure water with open purge valve Wrench 17 mm Wash bottle containing isopropanol Part number Description 1 3150 0942 Filter cup Whenever replacing the filters on the multi assembly is required because of a pump seal change or because excessive back pressure indicates the need for a replacement make sure to replace the filter cup first and then the filter frit see Exchanging the Filter Frit on page 122 after having pumped another 200 ml of solvent It is not possible to clean a contaminated filter cup in an ultrasonic bath because any contamination of the filter is normally permanent 1 Using a 17 mm wrench loosen filter screw on the multi assem
46. Using the Preparative Pump Hints for Successful Use of the Preparative Pump 52 Solvent Information 53 Prevent Blocking of Solvent Filters 54 Algae Growth in HPLC Systems 55 How to Prevent and or Reduce the Algae Problem 56 Setting Up the Pump 57 Setting up the Preparative Pump Agilent ChemStation 57 ee Agilent Technologies 51 4 Using the Preparative Pump Hints for Successful Use of the Preparative Pump 52 Flush the pump extensively when changing to a new solvent The system pressure must be higher than 20 bar at the pump outlet for optimum performance of the pump Place the solvent cabinet with the solvent bottles always on top of the preparative pump or at a higher level Prevent blocking of solvent inlet filters never use the pump without solvent inlet filters Growth of algae should be avoided see Prevent Blocking of Solvent Filters on page 54 Regularly clean the filter cup and the filter frit installed in the multi assembly A back pressure greater than 10 bar when pumping pure HPLC grade water at a flow rate of 50 ml min while the EMPV is open indicates that one of the two filters is blocked or that the EMPV does not switch open properly Make sure to follow the correct procedures for cleaning filters see Simple Repairs on page 114 and the EMPV see Prep Pump EMPV Cleaning Description on page 103 Always clean the pump s filters after exchanging seals Confirm that the pump and t
47. actions 1 Defective or disconnected index cable Ensure the connector connected and seated correctly 2 Defective Pump drive defective index board Exchange the pump drive assembly on pump drive 3 Defective main board Exchange the main board Position Error The motion control of the pump drive could not reach the desired condition within a certain time frame The deviation from the desired position exceeded the error limit Probable cause Suggested actions 1 Blocked flow path Ensure that there is no blockage in the flow path before the pressure sensor 2 Mechanical blockage of the pump drive Exchange the pump drive assembly assembly Agilent 1260 Infinity Preparative Pump User Manual 87 6 Troubleshooting and Diagnostics Spurious Index The pump motion control expects one and only one index signal for every turn of the drive wheel This error message is generated if this signal is received more often than within one complete turn of the drive wheel Probable cause Suggested actions 1 Missing reflections to light switch because Ensure the drive wheel is clean of contamination on drive wheel 2 Defective Pump drive Exchange the pump drive assembly 3 Defective main board Exchange the main board Encoder not Connected There is an encoder wheel mounted to the shaft of the pump motor that gives pulses to the motion control according to the rotation of the shaft The error message is generated if the short bridge o
48. ak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Agilent 1260 Infinity Preparative Pump User Manual Introduction to the Preparative Pump 1 Interfaces Special Interfaces Some 1260 Infinity modules have module specific interfaces connectors They are described in the module documentation Agilent 1260 Infinity Preparative Pump User Manual 21 1 22 Introduction to the Preparative Pump Interfaces Agilent 1260 Infinity Preparative Pump User Manual Agilent 1260 Infinity Preparative Pump User Manual 2 Site Requirements and Specifications Site Requirements 24 Power Consideration 24 Power Cords 25 Bench Space 26 Environment 26 Physical Specifications 27 Performance Specifications 28 ee Agilent Technologies 23 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimum performance of the instrument P
49. ake sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting Agilent 1260 Infinity Preparative Pump User Manual 169 10 Appendix When working w
50. ansfer The two connectors for the CAN bus are used for internal Agilent 1260 Infinity module data transfer and synchronization The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as common shut down prepare and so on The RS 232 connector may be used to control the preparative pump from a computer through an RS 232 connection using appropriate software This connector needs to be activated by the 8 bit configuration switch module in the upper right corner of the rear of the pump The software needs the appropriate drivers to support this communication See your software documentation for further information The power input socket accepts a line voltage of 100 240 Volts AC 10 with a line frequency of 50 60 Hz max power consumption is 250 VA Volt Amps There is no voltage selector on your preparative pump because the power supply has wide ranging capability There are no externally accessible fuses The security lever at the power input socket prevents that the preparative pump cover is taken OFF when line power is still connected The interface BCD board slot is used for external contacts BCD output and for LAN communication The CAN DC out provides 24 Volts DC power for external CAN devices like a switch valve Max permanent power consumption is 100 mA channel or 2 A for 2 sec Agilent 1260 Infinity Preparative Pump User Manual 39
51. be reorder No 5042 2461 1 Capillary 3 pump outlet to system G1361 67302 1 Bottle AMBER 2 0 9301 6341 1 Wrench open end 14 17 mm 8710 2435 1 Wrench open end 1 4 5 16 8710 0510 2 Wrench open end 7 16 3 8 8710 0972 1 Hex key 3mm 8710 2411 1 Hex key 4 mm 87102392 1 Pair of tweezers 5022 2195 1 Priming Syringe re order no pck of 10 5062 8534 1 Adapter for Priming Syringe 9301 1337 1 Tubing for Priming Syringe G1361 87300 1 Spare bottle inlet filter frit 3150 0944 1 Spare frit adapter for 4 7 mm OD tubing G1361 23205 1 Glass stop valve for stopped pumps 5042 6464 1 Spare filter cup 3150 0942 1 Spare SST frit 2 pm 5022 2192 1 CAN cable 1 m 5181 1519 1 Union preparative 5022 2133 1 Sanding paper no PN 2 32 Agilent 1260 Infinity Preparative Pump User Manual Installing the Pump 3 Gradient Kit Contents Table9 Gradient Kit Contents G1361 68707 shipped with every additional pump ina gradient system Description Part Number Quantity Bottle head assembly G1361 60022 1 Tube seal wash 0890 1764 1 Waste tube 5042 2461 1 Bottle clear 2 0 I 9301 6342 1 Capillary 5 pump A to T union G1361 67304 1 Capillary 6 T union to inlet of mixer G1361 67305 1 Female T union 0100 1016 1 Mixer 79835 87330 1 Capillary 7 pump B to T union G1361 67306 1 Spare bottle inlet filter frit 3150 0944 1 Spare frit adapter for 4 7 mm OD tubing G1361 23205 1 Glass stop valve for stopped pumps 5042 6464 1 Spare filter cup 3150 0942 1 Spare SST frit
52. bly 2 Carefully pull out filter screw and filter cup Agilent 1260 Infinity Preparative Pump User Manual Maintenance 7 Simple Repairs 3 Remove the filter cup from the screw by holding the screw and filter and pulling them apart NOTE Generally all components which are in direct contact with solvents should be handled wearing gloves Filter screw Filter cup Figure 25 Exchanging the Filter Cup 4 Before reinserting the new filter screw into the multi assembly wash the filter housing thoroughly with a wash bottle containing Isopropanol to remove any contamination 5 Install a new filter into the filter screw 6 Reinsert the filter and filter screw and turn until finger tight then another 1 8 turn using a 17 mm wrench NOTE Do not overtighten the filter screw Excessive force on the filter screw doesn t help in case of a leak 7 Pump about 200 ml of solvent before replacing the filter frit installed at the multi assembly the filter frit between the outlet of the multi assembly and the EMPV see Exchanging the Filter Frit on page 122 Agilent 1260 Infinity Preparative Pump User Manual 121 7 Maintenance Exchanging the Filter Frit Whenever replacing the filters on the multi assembly is required because of a pump seal change or because excessive back pressure indicates the need for a replacement make sure to replace the filter cup first see Exchanging the Fil
53. compensation sensor open 79 compensation sensor short 80 composition cluster partner lost 90 EMPV Missing 89 encoder not connected 88 fan failed 80 ignition without cover 82 82 index missing 87 leak sensor open 78 leak sensor short 79 leak 78 motor direction 86 motor driver fault 85 motor driver temperature too high 84 motor maximum power fault 86 position error 87 pressure above upper limit 82 pressure below lower limit 83 pressure sensor not connected 83 pump off while analysis 87 remote timeout 76 seal wash current low 91 sealwash 92 shut down 76 solvent counter zero 91 spurious index 88 timeout 75 wait for encoder position timed out 92 wait for pressure timed out 90 wait for volume timed out 90 wrong data from pressure sensor 84 exchanging EMPV 114 filter cup 114 120 filter plate 114 122 180 filter 114 114 fitters 120 122 inlet valve 117 inlet valve 114 interface board 131 outlet valve 117 outlet valve 114 pistons 114 126 plungers 114 126 purge valve 114 seal wash pump cartridge 125 seal wash pump cassette 114 seals 114 126 valves 114 114 117 external contacts BCD board 155 155 F fan drive 151 fan failed 80 features GLP 28 instrument layout 12 safety and maintenace 28 filter cup 114 120 filter plate 114 122 filters 120 122 firmware updates 132 upgade downgrade 132 first analysis 49 flow precision 28 flow range 28 flow cell 176 solvent information
54. compounds PEEK Pump head SST sapphire Inlet Outlet Valves SST sapphire ruby PEEK EMPV SST ruby sapphire PEEK Filter Cup SST Filter Plate SST PEEK Pressure Sensor SST PEEK Capillaries SST For pump specifications see Site Requirements on page 24 How Does Compressibility Compensation Work The compressibility of the solvents in use will affect retention time stability when the back pressure in the system changes for example ageing of column In order to minimize this effect the pump provides a compressibility compensation feature which optimizes the flow stability according to the solvent type The compressibility compensation is set to a default value and can be changed through the user interface Without a compressibility compensation the following will happen during a stroke of the first piston The pressure in the piston chamber increases and the volume in the chamber will be compressed depending on backpressure and solvent type The volume displaced into the system will be reduced by the compressed volume When a compressibility compensation value for the pump head is set the pump processor calculates a compensation volume that depends on the system pressure and the selected compressibility value The pump has a fixed stroke To compensate for compressibility losses the speed of the piston movement has to be varied during the different parts of each stroke Agilent 1260 Infinity Preparative Pump User Man
55. ct with mobile phase 10 message ignition without cover 82 82 etal bottles 42 45 issing parts 30 otor driver temperature too high 84 otor maximum power fault 86 otor direction 86 otor drive 150 MPS main power supply 164 MPS main power supply 13 m m m motor driver fault 85 m m m no motor motion 91 no pump head 89 non operating altitude 27 non operating temperature 27 not ready condition 73 0 onboard battery 151 operating range 28 operating Altitude 27 operating temperature 27 organic solvents 66 outet valve 117 outlet valves 114 overview of parts 112 overview hydraulic path 9 preparative pump 8 Agilent 1260 Infinity Preparative Pump User Manual P parts basis kit 145 bottle head assembly 140 damaged 30 hydraulic path 142 missing 30 pump head 138 solvent cabinet 140 performance specification 28 pH range 28 physical specifications 27 pistons 114 126 plungers 52 114 126 position error 87 power supply indicator 72 power supply description 164 power switch 37 power consideration 24 power consumption 27 39 power cords 25 power input 39 power supply description 13 wide ranging capability 39 preparative pump main board PPM 150 preparative pump delivery checklist 31 31 preparative pump connections 38 prerun condition 73 pressure above upper limit 82 pressure below lower limit 83 pressure sensor not connected 83 pressure test 94 pressure converter 150 pressure pulsation 67
56. d motor amplifier Electromechanical Proportional Valve The EMPV consists of an electro magnetic solenoid driven by a PWM Pulse Width Modulator Pressure Converter This block comprises a filter and amplifier for the pressure sensor signal an A D converter with integrated multiplexer Leak Converter This block comprises a PTC for the leak identification and a NTC for the ambient temperature measurement This assures that temperature changes are not identified as leak A leak would cool down the PTC and its change in resistance results in a leak signal Agilent 1260 Infinity Preparative Pump User Manual Configuring the Preparative Pump 9 Fan Drive The revolution of the fan is controlled by the main processor depending on the internal heat distribution in the preparative pump The fan provides a PWM signal which is proportional to the revolution This fan status signal is used for diagnostics Seal Wash Pump Drive The drive comprises a current control and full bridge driver for the seal wash pump Pump head ID Module The pump head ID Module is used to write pump head specific information onto a tag that is installed in the pump head Index Board The Index board is used to sense the movement and positioning of the pump drive It also comprises calibration information of the drive Electronic Fuses The valve circuit is electronically fused on the board Any error on the board or shortages of the valve will activate the elec
57. distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained Agilent 1260 Infinity Preparative Pump User Manual 19 1 20 Introduction to the Preparative Pump by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal level are defined as e standard TTL levels 0 V is logic true 5 V is false e fan out is 10 e input load is 2 2 kOhm against 5 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 volt power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 V with respect to the ground terminal Table 3 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example le
58. e Pump User Manual Using the Preparative Pump 4 Actual Volume Set the actual volume after refilling the solvent bottle When turning on the pump the current filling will be calculated based on the flow rate and the run time of the pump Total Volume This value is used to calculate the filling of the bottle for the display in the system diagram You can select not to start the run if the current fill level is below a user defined value and to turn off the pump before it is running out off solvent Solvent Bottle Filling A Instrument 1 DED 02001 x Actual Volume Total Volume A 40 95 liter 2 00 liter JV Prevent analysis if level falls below 0 100 liter IV Tum pump off if unning out of solvent Cancel Help Figure 21 Solvent Bottle Filling dialog box The Solvent Bottle Filling dialog box is also available for channel B on gradient pump systems Agilent 1260 Infinity Preparative Pump User Manual 63 4 Using the Preparative Pump Setting Up the Pump 64 Agilent 1260 Infinity Preparative Pump User Manual Agilent 1260 Infinity Preparative Pump User Manual 5 Optimizing Performance How to Configure the Seal Wash Function 66 Water or Aqueous Buffer Solutions 66 Organic Solvents 66 How to Optimize the Compressibility Compensation Setting 67 ee Agilent Technologies 65 5 Optimizing Performance How to Configure the Seal Wash Function Always use the seal wash function which
59. ential ignition of flammable vapors or solvents gt To avoid electrostatic discharge never use the pump without grounded metal solvent bottles gt Do not use metal bottles without appropriate grounding 42 Agilent 1260 Infinity Preparative Pump User Manual Installing the Pump 3 1 Remove the front cover by pressing the snap fasteners on both sides Figure 10 Removing the Front Cover Place the solvent cabinet on top of the module Place the bottle into the solvent cabinet and place a bottle head assembly into the bottle Ground the solvent bottle 4 Connect the solvent tubes from the bottle head assemblies to the inlet adapters of the pump Fix the tubes in the clips of solvent cabinet and preparative pump 5 By holding a piece of sanding paper around the waste tubing connect it to the electromagnetic purge valve EMPV and place it into your waste system 6 Ifthe preparative pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack connect the corrugated waste tube to the waste outlet of the pump leak handling system In order to guarantee an error free handling of any leakage that may occur in the system all modules of a stack must be thoroughly aligned on top of each other Outstanding knobs at the rear of the top covers of the modules and holes as counterparts at the rear of the bottom covers of the modules will help to align the instruments nicely on top of each other by mak
60. es Yes No Yes G1329A STD 2 Yes No Yes Yes No Yes THERMOSTAT for G1329B STD SL G1330A B G2260A PREP G1364A FRC 2 Yes No Yes Yes No Yes THERMOSTAT for G1367A B C D G1330A B WPS CAN DC OUT for G1377A pWPS CAN slaves G2258A D LOOP Detectors G1314A B VWD 2 Yes No Yes Yes 1 Yes G1314C VWD SL 2 Yes No No Yes 1 Yes G1314D VWD 2 No Yes No Yes 1 Yes G1314E VWD SL 2 No Yes No Yes 1 Yes 16 Agilent 1260 Infinity Preparative Pump User Manual Introduction to the Preparative Pump 1 Table 2 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN GPIB RS 232 Analog APG Special optional on board Remote G1315A B DAD 2 Yes No Yes Yes 2 Yes G1365A B MWD G1315C DAD SL 2 No Yes No Yes 2 Yes G1365C MWD SL G1315D DAD G1365D MWD G1321A FLD 2 Yes No Yes Yes 1 Yes G1362A RID G4280A ELSD No No NO No Yes Yes Yes EXT Contact AUTOZERO Others G1316A TCC No No No A Yes No Yes G1316B TCC SL No No No A Yes No Yes G1322A DEG No No No No No No Yes AUX G1379A DEG No No No No Yes No No AUX G4240A CHIPCUBE 2 Yes No No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED e CAN connectors as interface to other Agilent 1200 Infinity Series modules e GPIB connector as interface to the Agilent ChemStation e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e analog output connector s for signal output and e interface slot for specific interfacing ex
61. ess steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCly O gt 2COCly 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing strong complexing agents e g EDTA Mixtures of carbon tetrachloride with 2 propanol or THF dissolve stainless steel Agilent 1260 Infinity Preparative Pump User Manual 53 4 Using the Preparative Pump Prevent Blocking of Solvent Filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module This is especially true for aqueous solvents or phosphate buffers pH 4 to 7 The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the module e Use a sterile if possible amber solvent bottle to slow down algae growth e Filter solvents through filters or membranes that remove algae e Exchange solvents every two days or refilter e If the application permits add 0 0001 0 001M sodium azide to the solvent e Place a layer of argon on top of your solvent e Avoid exposure of the solvent bottle to
62. finity Preparative Pump User Manual Agilent 1260 Infinity Preparative Pump User Manual 3 Installing the Pump Unpacking the Preparative Pump 30 Damaged Packaging 30 Delivery Checklist 30 Basic Kit Contents 32 Gradient Kit Contents 33 Optimizing the Stack Configuration 34 Preparative System 34 Installing the Preparative Pump 36 Connecting Modules and Control Software 40 Connecting Agilent 1260 Infinity modules 40 Connecting control software control module 41 Flow Connections for a Single Isocratic Preparative Pump 42 Flow Connections for a Dual Binary Gradient Preparative Pump 45 Get the System Ready for the First Analysis 49 Priming your preparative LC system with the pump 49 ee Agilent Technologies 29 3 Installing the Pump Unpacking the Preparative Pump 30 Damaged Packaging Upon receipt of your quaternary pump inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged save them until the contents have been checked for completeness and the quaternary pump has been mechanically and electrically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carrier s inspection Delivery Checklist Ensure all parts and materials have been delivered with the preparative pump The delivery checklist is shown in Table 6 on page 31 till Table 9 on page 33 To aid in parts identification please see
63. from the flow path 2 Check your system for leak tightness with the help of the built in pressure test see Pressure Test Prep Pump Pressure Test Description on page 94 3 Refer to Optimizing Performance on page 65 for optimizing your pump s performance 4 Before starting an analysis pump until you receive a stable pressure signal from your pump and until your detector baseline has stabilized normally no more than five minutes Low ripple is a vital sign for a smoothly operating pump and a stable system Agilent 1260 Infinity Preparative Pump User Manual 49 3 50 Installing the Pump 5 When the pumping system has been turned OFF for a certain time for example overnight repeat Step 3 step 4 on page 49 Table 10 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation When switching between reverse phase and normal phase both times After an installation To clean the system when using buffers After a solvent change After the installation of normal phase solvents lsopropanol Ethanol or Methanol Bidistilled water Hexane 5 lsopropanol Best solvent to flush air out of the system Alternative to lsopropanol second choice if no lsopropanol is available Best solvent to re dissolve salts Good wetting properties Agilent 1260 Infinity Preparative Pump User Manual Agilent 1260 Infinity Preparative Pump User Manual 4
64. g a flow out through the waste tubing of the EMPV while the EMPV should be closed Agilent 1260 Infinity Preparative Pump User Manual 97 6 Troubleshooting and Diagnostics Leak Test Prep Pump Leak Test Description The leak test is a quick built in test designed to demonstrate the pressure tightness of the pump The test involves monitoring the pump drive steps needed to build up a pressure of 350 bar and the flow rate required to keep a constant pressure of 350 bar as the preparative pump runs through a predefined pumping sequence The resulting flow rate provides information about the pressure tightness of the pump Each step is separately performed and evaluated separately for piston 1 and piston 2 Step 1 The test begins with an automated flushing of the pump at a flow rate of 25 ml min with an open EMPV Step 2 The EMPV is closed and next piston 1 begins pumping solvent with a flow rate of 2 ml min until reaching a pressure of 150 bar then the pump delivers at a flow rate of 0 2 ml min until reaching a pressure of 250 bar then the pump delivers at a flow rate of 0 1 ml min until reaching a pressure of 345 bar then the pump switches into its Keep pressure mode Step 3 The flow rate to compensate for leaks should from this point onwards be no more than 10 ul minute Step 4 Steps 2 and 3 are automatically repeated for piston 2 98 Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagno
65. ge to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Agilent 1260 Infinity Preparative Pump User Manual Contents Contents Introduction to the Preparative Pump 7 Introduction to the Preparative Pump 8 Instrument Layout 12 The Electronics 13 Electrical Connections 14 Interfaces 16 Site Requirements and Specifications 23 Site Requirements 24 Physical Specifications 27 Performance Specifications 28 Installing the Pump 29 Unpacking the Preparative Pump 30 Optimizing the Stack Configuration 34 Installing the Preparative Pump 36 Connecting Modules and Control Software 40 Flow Connections for a Single Isocratic Preparative Pump 42 Flow Connections for a Dual Binary Gradient Preparative Pump 45 Get the System Ready for the First Analysis 49 Using the Preparative Pump 51 Hints for Successful Use of the Preparative Pump 52 Solvent Information 53 Prevent Blocking of Solvent Filters 54 Algae Growth in HPLC Systems 55 Setting Up the Pump 57 Agilent 1260 Infinity Preparative Pump User Manual Contents 5 Optimizing
66. h affects correct operation of the module Usually an error condition requires attention for example leak defective internal components An error condition always interrupts the analysis A flashing yellow status indicator indicates that the module is in its resident mode Call your local service provider for assistance upon observing this error condition A flashing red status indicator indicates a severe error during the startup procedure of the module Call your local service provider for assistance upon observing this error condition Agilent 1260 Infinity Preparative Pump User Manual 73 6 Troubleshooting and Diagnostics User Interfaces Depending on the User Interface the available test vary Some descriptions are only available in the Service Manual Table 12 Test Functions available vs User Interface Test ChemStation Instant Pilot Agilent Lab G4208A Monitoring amp Diagnostic Software Pressure Test Yes No Yes Leak Test Yes No Yes EMPV Cleaning Yes No Yes 74 Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair frit exchange or exchange of consumables required In the event of such a failure the red status indicator at the front of the module is switched
67. he original Agilent pump head with RF tag Exchange PPM board 89 6 90 Troubleshooting and Diagnostics Wait for Volume Timed Out The wait time limit until a given volume has been pumped has been exceeded Probable cause Suggested actions 1 Wrong limit set for the time limit Set limit accordingly 2 Flow setting too low Set flow accordingly Wait for Pressure Timed Out The limit for waiting until a given pressure is reached has been exceeded Probable cause Suggested actions 1 Wrong limit set for the time limit Set limit accordingly 2 Leak Check for leaks with the help of the built in pressure or leak test and correct them where necessary Composition Cluster Partner Lost Communication to a composition cluster partner was interrupted In a gradient system two or more isocratic preparative pumps can be linked together in a cluster acting as one gradient pump The error message is generated if the communication between the partners of a cluster is interrupted Probable cause Suggested actions 1 Composition cluster partner was turned OFF Turn ON composition cluster partner 2 CAN cable to composition cluster partner Reconnect or replace CAN cable disconnected 3 Defective CAN bus Replace PPM main board Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 Solvent Counter Zero The volume counter for the bottle filling reached zero Probable cause Suggested
68. he pump inlet outlet valves 6 Large leaks visible at filters multi assembly capillaries or EMPV 7 Leaky pressure sensor 8 Leaking seal or scratched plungers of channel 1 or channel 2 Agilent 1260 Infinity Preparative Pump User Manual Suggested actions Use Isopropyl alcohol only Ensure all fittings are tight or exchange capillary Run the EMPV cleaning procedure Ensure the pump head screws are tight Tighten or exchange the pump inlet outlet valves Ensure the leaky components are installed tightly Exchange the component if required Run the EMPV cleaning procedure Exchange the multi assembly Exchange the pump seals in both channels Check the plungers for scratches Exchange if scratched 101 6 102 Troubleshooting and Diagnostics Maximum Allowed Flow Rate exceeded for Piston 1 Probable cause 1 Externally leaking inlet or outlet valve in channel 1 2 Internally leaking inlet valve in channel 1 back flow 3 Internally leaking outlet valve in channel 2 back flow 4 Leaking seal or scratched plunger in channel 1 5 Loose or leaky fitting or capillary or fitting in channel 1 Suggested actions Ensure the valves are installed correctly Tighten the valves Clean or replace the inlet valve in channel 1 Clean or replace the outlet valve in channel 2 Exchange the pump seals in channel 1 Check the plunger for scratches Exchange if scratched Tighten fitting or
69. he resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Exchange the main board Fan Failed The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated Probable cause Suggested actions 1 Fan cable disconnected Ensure the fan is connected correctly 2 Defective fan Exchange fan 3 Defective main board Exchange the main board 4 Improperly positioned cables or wires Ensure the fan is not mechanically blocked obstructing fan blades 80 Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 Open Cover Safety Do not remove instrument covers when operating Before the instrument is switched ON all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protectio
70. he rest of the system are completely leak tight by performing the built in Leak test see Prep Pump Leak Test Description on page 98 and Pressure test see Prep Pump Pressure Test Description on page 94 regularly When using buffer solutions flush the system with plenty of water to remove all buffer solution from the entire system before switching it OFF or before changing to an organic solvent Always use the seal wash function Check the pump seals Scratched plungers will lead to micro leaks and will decrease the lifetime of the seal plungers for scratches when changing the piston For the generation of gradients in systems with multiple pump setups make sure that none of the pumps delivers less than a minimum flow rate of 5 ml min at any time during the gradient run in order to achieve best performance Agilent 1260 Infinity Preparative Pump User Manual Using the Preparative Pump 4 Solvent Information Always filter solvents through 0 4 um filters small particles can permanently block the capillaries and valves Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric and nitric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainl
71. iagnose signals 153 9 Configuring the Preparative Pump Preparative Pump Main Board PPM Encoder SMF fuse Motor T Power supply lambda eea 10A 24V 5V 5V 2A 4 15V 0 Index I Power switch status status Electronic Hardware 24VF EEN 2 oe fuse l switch ___ 24 VF Seal wash Seal wash pump pump DV 15V Teak converter Pump Control Med Fan drive Fan drive head ID a 15V Pressure Pressure converter sensor Leak sensor Fan CAN P4 P5 RS232 P6 2avean Power one Remote P7 switch MIO P25 CAN DG Figure 36 Interconnection Diagram CSM Board 154 Agilent 1260 Infinity Preparative Pump User Manual Configuring the Preparative Pump 9 Optional Interface Boards BCD External Contact Board The Agilent 1260 Infinity modules have one optional board slot that allows to add an interface board to the modules Some modules do not have this interface slot Refer to Interfaces on page 16 for details Table 21 Optional Interface Boards Description Part Number BCD Board G1351 68701 Fuse 250 mA four are on the board 2110 0004 The BCD board provides a BCD output for the bottle number of the Agilent 1260 Infinity autosampler and four external contacts The external contact closure contacts are
72. ice Manual Inlet valves see Removing an Inlet Ball Valve Bottom of the Pump Head on page 117 Pump head see Removing and Disassembling the Pump Head Assembly on page 126 Outlet valves see Removing an Outlet Ball Valve Top of the Pump Head on page 118 Pump drive see Exchanging a Pump Drive in the Service Manual Power supply see Exchanging a Power Supply in the Service Manual PPM board see Exchanging a PPM Board in the Service Manual Fan see Exchanging a Fan in the Service Manual Electromagnetic purge valve EMPV see Exchanging a EMPV in the Service Manual Seal wash pump see Exchanging the Seal Wash Pump Cartridge on page 125 Agilent 1260 Infinity Preparative Pump User Manual 113 7 Maintenance Simple Repairs The procedures described in this section can be done with the preparative pump in place in the system stack Table 13 Simple Repair Procedures Procedure Symptom Potential causes Removing an Inlet Ball Valve Bottom of the Pump Head on page 117 Exchanging the Filter Cup on page 120 Exchanging the Filter Frit on page 122 Exchanging the Seal Wash Pump Cartridge on page 125 Removing and Disassembling the Pump Head Assembly on page 126 Reassembling the Pump Head Assembly on page 129 If leaking or not opening closing properly Excessive back pressure with open EMPV unstable pressure Excessive back pressure with open EMPV
73. iction partial mechanical blockage in Remove the pump head assembly Ensure there the pump drive assembly is no mechanical blockage of the pump head assembly or pump drive assembly 2 Partial blockage of the flowpath in front of Ensure the outlet valve is not blocked the pressure sensor Ensure the capillaries and frits between the pump head and pressure sensor at the rear of the multi assembly are free from blockage Clean the filters in the multi assembly 3 Defective drive assembly Exchange defective drive assembly 4 Defective PPM board Exchange the PPM board 84 Agilent 1260 Infinity Preparative Pump User Manual Motor Drive Fault Troubleshooting and Diagnostics 6 The motor drive chip has issued a fault signal The current drawn by the pump motor exceeded the maximum limit or under voltage lockout Blockages in the flowpath are usually detected by the pressure sensor which result in the preparative pump switching OFF when the upper pressure limit is exceeded If a blockage occurs before the pressure sensor i e the pressure increase cannot be detected by the pressure sensor the preparative pump will continue to pump As pressure increases the pump drive draws more current When the current reaches the maximum limit the preparative pump is switched OFF and the error message is generated Probable cause 1 Flow path blockage in front of the pressure sensor 2 Blocked outlet ball valve 3 High friction pa
74. ing them slide into the correct position This will then allow any possible spills to be routed safely from one instrument to the next one with the help of the built in leak drainage system Each module has a funnel to catch drops coming from the above instrument from there spills a lead through a waste drain tube into the leak pan and out from there through an outlet on to the next module Agilent 1260 Infinity Preparative Pump User Manual 43 3 Installing the Pump Flow Connections for a Single Isocratic Preparative Pump 7 Connect the Outlet Capillary G1361 67302 to the electromagnetic purge valve EMPV and make the necessary connections the other side of the capillary normally a capillary to an injector or injection valve 8 Install the seal wash tubings as shown in Figure 11 on page 44 You need a tubing from your seal wash solvent bottle to the seal wash pump one from there to the pump head and one from the pump head to waste 9 Install the front cover 10 Press in the power switch to turn ON the preparative pump From solvent bottle A Tube clip Leak funnel Outlet ball valves Tubing Seal wash Leak drainage to waste a ce S Inlet ball valves EMPV out to g l next module P AS CS ey T a From seal wash solvent bottle to seal wash pump EMPV to waste Seal wash pump to pump head Waste outlet corrugated waste tubing Figure 11 Flow connections to the preparative pump 44 Agilent
75. ith solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 170 Agilent 1260 Infinity Preparative Pump User Manual Appendix 10 Safety Symbols Table 28 Safety Symbols Symbol Description gt e The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up WARNING es alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions Agilent 1260 Infinity Preparative Pump User Manual 171 10 Appendix The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC
76. ized by the operating software from Agilent Technologies RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module next to the GPIB connector Agilent 1260 Infinity Preparative Pump User Manual Introduction to the Preparative Pump 1 The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Instrument PC DCD 1 1 DCD RX 2 o2 2 RX TX 3 X 3 TX DTR 4 T 4 DTR GND 5 y 5 GND DSR 6 6 DSR RTS 7 y 7 RTS CTS 8 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 4 RS 232 Cable Analog Signal Output The analog signal output e g detector signal or pump pressure signal can be distributed to a recording device For details refer to the description of the main board of the module APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a
77. k diagram 152 153 pump controller board 152 153 blocking of filters 52 board HP JetDirect card 157 boards LAN card 157 bottle head assembly parts 140 buffer application 52 buffer solutions 66 C cable connecting APG remote 35 connecting CAN 35 connecting GPIB 35 connecting LAN 35 connecting the ChemStation 35 connecting the power 35 CAN interface 159 CAN bus 39 CAN DC out 39 CAN interface 18 cleaning 111 compensation sensor open 79 compensation sensor short 80 composition cluster partner lost 90 compressibility compensation 28 compressibility compensation 10 67 condensation 26 connections flow 45 connections Agilent 1260 Infinity Preparative Pump User Manual electrical 38 control and data evaluation 28 control software 41 D damaged parts 30 delivery checklist preparative pump 31 31 details preparative pump 10 Diagnostic software 70 dimensions 27 disassembling pump head assembly 114 126 E early maintenance feedback EMF 12 electrical connections descriptions of 14 electromagnetic proportioning valve 150 electronic fuses 151 electrostatic discharge ESD 109 131 EMPV cleaning procedure 103 EMPV fuse blown 89 EMPV Missing 89 EMPV 114 150 encoder not connected 88 environment 24 26 error messages EMPV fuse blown 89 error message 24V supply for external devices low 93 error messages 179 Index no motor motion 91 nopumphead 89 error condition 73 error messages
78. kat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA Agilent 1260 Infinity Preparative Pump User Manual 173 10 Appendix Radio Interference Cables supplied by Agilent Technoligies are screened to provide opitimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 174 Agilent 1260 Infinity Preparative Pump User Manual Appendix 10 Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test Agilent 1260 Infinity Preparative Pump User Manual 175 10 Appendix Solvent Information 176 Flow Cell To protect o
79. leak sensor is connected correctly board 2 Defective leak sensor Exchange the leak sensor Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 Leak Sensor Short The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Exchange the leak sensor 2 Leak sensor incorrectly routed being Exchange the leak sensor pinched by a metal component Compensation Sensor Open The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Exchange the main board Agilent 1260 Infinity Preparative Pump User Manual 79 6 Troubleshooting and Diagnostics Compensation Sensor Short The ambient compensation sensor NTC on the main board in the module has failed short circuit T
80. lvent inlet filter 52 solvent cabinet 52 solvent filters prevent blocking 54 solvent information 52 53 176 solvents 1 6 special interface 21 specification performance 28 physical 27 spurious index 88 stack configuration 35 rearview 35 status indicators 72 supply for external devices low 93 T temperature sensor 78 timeout 75 U unpacking the pump 28 V valves 114 114 117 voltage range 27 voltage selector 39 wW wait for encoder position timed out 92 wait for pressure timed out 90 wait for volume timed out 90 weight 27 wide ranging capability 39 wrench 1 4 inch 96 99 wrong data from pressure sensor 84 Agilent 1260 Infinity Preparative Pump User Manual Index Agilent 1260 Infinity Preparative Pump User Manual 183 www agilent com In This Book This manual contains technical reference information about the Agilent 1260 Infinity Preparative Pump The manual describes the following e introduction e requirements e installation e using the pump e optimizing performance e troubleshooting and diagnostics e maintenance e parts and materials e identifying cables e configuring the pump e legal safety and warranty information Agilent Technologies 2006 2007 2011 Printed in Germany 02 2011 G1361 90012 ea Agilent Technologies
81. ly is used to turn OFF output voltages if the temperature exceeds the acceptable limit for example if the cooling fan of the instrument fails To reset the main power supply to normal operating conditions turn the instrument OFF wait until it is approximately at ambient temperature and turn the instrument on again The following table gives the specifications of the main power supply Table 27 Main Power Supply Specifications Maximum power Line input Output 1 Output 2 Output 3 Output 4 Output 5 130 W Continuous output 100 240 volts AC 10 line Wide ranging frequency of 50 60 Hz 24V 4 5 A maximum Total power consumption of 24 V and 36 V must not exceed 107 W 36 V 2 5 A maximum 5V 3A 15V 03A 15V 03A Agilent 1260 Infinity Preparative Pump User Manual 165 9 Configuring the Preparative Pump Main Power Supply Assembly 166 Agilent 1260 Infinity Preparative Pump User Manual Agilent 1260 Infinity Preparative Pump User Manual 10 Appendix General Safety Information 168 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 172 Lithium Batteries Information 173 Radio Interference 174 Sound Emission 175 Solvent Information 176 Agilent Technologies on Internet 178 ee Agilent Technologies 167 10 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of
82. mmon Agilent 1260 Infinity Preparative Pump User Manual 55 4 Using the Preparative Pump Symptoms Observed with the Agilent 1260 Infinity HPLC In contrast to the HP 1090 and HP 1050 Series HPLC systems which use helium degassing algae have a better chance to grow in systems such as the Agilent 1260 Infinity Series where helium is not used for degassing most algae need oxygen and light for growth The presence of algae in the Agilent 1260 Infinity Series can cause the following to occur Short lifetime of solvent filters bottle head assembly A blocked solvent filter in the bottle especially when only partly blocked is more difficult to identify and may show up as gradient performance problems intermittent pressure fluctuations etc Algae growth may also be the possible source for failures of the ball valves and other components in the flow path How to Prevent and or Reduce the Algae Problem 56 Always use freshly prepared solvents especially use demineralized water which was filtered through about 0 2 um filters Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle part number 9301 1450 supplied with the instrument for your aqueous mobile phase If possible add a few mg l sodium azide or a few percent organic solvent to the aqueous mobile phase Agilent 1260 Infinity Preparative Pump User Manual Using the Preparative Pum
83. n Preparative System Rear View If a G1330B ALS thermostat is part of the system it must always be installed underneath the Autosampler 35 3 Installing the Pump Installing the Preparative Pump Parts required Part number Description 1 G1361A Pump 1 Power cord for other cables see text below and Cable Overview in the Service Manual 1 Control Software ChemStation EZChrom OpenLab etc and or G4208A or a handheld controller Instant Pilot G1323B Preparations Locate bench space Provide power connections Unpack the pump WARNING Instrument is partially energized when switched off The power supply still uses some power even if the power switch on the front panel is turned off gt To disconnect the module from line unplug the power cord WARNING Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 36 Agilent 1260 Infinity Preparative Pump User Manual Installing the Pump 3 CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage
84. n a different module Switch off the system Restart the system and determine which module or modules are not recognized by the system Agilent 1260 Infinity Preparative Pump User Manual 77 78 Troubleshooting and Diagnostics Leak A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 3 Loose or leaking active inlet valve outlet ball Ensure pump components are seated correctly valve or EMPV If there are still signs of a leak exchange the appropriate seal active inlet valve outlet ball valve or EMPV 4 Defective pump seals Exchange the pump seals Leak Sensor Open The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the main Ensure the
85. n has been impaired the instrument must be made inoperative and be secured against any intended operation WARNING Open main cover The following procedures require opening the main cover of the instrument gt Always ensure the instrument is disconnected from the line power when the main cover is removed gt The security lever at the power input socket prevents that the instrument cover is taken off when line power is still connected WARNING Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed Agilent 1260 Infinity Preparative Pump User Manual 81 6 82 Troubleshooting and Diagnostics Open Cover The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is removed the pump is turned OFF within 4s for safety reasons and the error message is generated If the pump is started without cover this error message is also generated and the pump is not turned on Probable cause 1 The top foam was removed during operation or pump started without cover 2 Foam
86. n the connector is not sensed Probable cause Suggested actions 1 Defective or disconnected pump encoder Ensure the connector is clean and seated connector or cable correctly 2 Defective main board Exchange main board 88 Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 Purge Valve EMPV Fuse Blown The electronic fuse has detected that the current through the purge valve was too high Probable cause 1 Defective purge valve 2 Defective PPM board Suggested actions Exchange the drive of the EMPV Exchange the PPM board Electro Magnetic Proportional Valve EMPV Missing The EMPV in the preparative pump is missing or defective Probable cause 1 Disconnected or defective cable 2 Defective purge valve 3 Defective PPM board No Pump Head Suggested actions Ensure the connection cable is seated correctly Exchange the drive of the EMPV Exchange PPM board The pump head RF tag in the preparative pump was not found Probable cause 1 Pump head not installed correctly screws not secured or pump head not seated correctly 2 RF tag on pump head missing or defective 3 Wrong pump head connected 4 Defective PPM board Agilent 1260 Infinity Preparative Pump User Manual Suggested actions Install the pump head correctly Ensure nothing e g capillary is trapped between the pump head and body Install missing or replace defective RF tag Install t
87. not activating the sensor 3 Sensor defective 4 Rear ofthe module is exposed to strong direct sunlight Pressure Above Upper Limit Suggested actions Reinstall the top foam Replace the top foam Exchange the main board Ensure that the rear of module is not directly exposed to strong sunlight The system pressure has exceeded the upper pressure limit This error condition automatically results in the opening of the purge valve Probable cause 1 Upper pressure limit set too low 2 Blockage in the flowpath after the pressure sensor 3 Defective pressure sensor 4 Defective main board Suggested actions Ensure the upper pressure limit is set to a value suitable for the analysis Check for blockage in the flowpath Exchange the Multi Assembly Exchange the main board Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 Pressure Below Lower Limit The system pressure has fallen below the lower pressure limit Probable cause Suggested actions 1 Lower pressure limit set too high Ensure the lower pressure limit is set to a value suitable for the analysis 2 Air bubbles in the mobile phase Ensure bubble free flow Purge the preparative pump 3 Leak Inspect the pump head capillaries and fittings for signs of a leak Purge the module Run a pressure test to determine whether the seals or other module components are defective 4 Defective pressure sensor
88. olvent Inlet Filter Assembly for high filtering capacity 5065 4500 includes tubing and connectors 13 Replacement HiFlow Solvent Inlet Filter glass housing with 5065 9901 filter 140 Agilent 1260 Infinity Preparative Pump User Manual Parts and Materials for Maintenance 8 Solvent Cabinet and Solvent Inlet Parts Figure 33 Solvent Cabinet and Solvent Inlet Parts Agilent 1260 Infinity Preparative Pump User Manual 141 Parts and Materials for Maintenance Hydraulic Path 142 Table 18 Hydraulic Path Item Description Part Number 1 2 3 Tubing from solvent bottle see Solvent Cabinet and Solvent Inlet Parts on page 140 Tube clip Leak funnel Leak drainage same as item 7 re order 5 m Prep Capillary 3 basis kit EMPV out to next module SST 1 16 o d 0 6 mm i d 400 mm long Prep Capillary 4 multi assembly to EMPV SST 1 16 o d 0 5 mm i d 160 mm long Tubing PTFE 0 057 i d EMPV to waste re order 5 m Corrugated waste tube 120 cm re order 5 m Tubing Seal wash pump to pump head Tubing Seal wash solvent bottle to seal wash pump Tube adapter for item 8 and 9 Inlet ball valve see Pump Head Assembly on page 138 Outlet ball valve see Pump Head Assembly on page 138 Tubing Seal wash to waste Prep Capillary 1 Outlet valve 1 to multi assembly top SST 1 16 o d 0 6 mm i d 173 mm long Prep Capillary 2 Outlet valve 2 to multi assembly bo
89. ondition will be reset automatically when the excessive current is not drawn from the external 24 CAN DC out port any more 2 Defective main board Exchange main board Agilent 1260 Infinity Preparative Pump User Manual 6 93 6 Troubleshooting and Diagnostics Pressure Test Prep Pump Pressure Test Description The pressure test is a quick built in test designed to demonstrate the pressure tightness of the system The test involves monitoring the flow rate required to keep a constant pressure of 350 bar as the preparative pump runs through a predefined pumping sequence The resulting flow rate provides information about the pressure tightness of the system Step 1 The test begins with an automated flushing of the pump at a flow rate of 25 ml min with an open EMPV Step 2 The EMPYV is closed and next piston A begins pumping solvent with a flow rate of 0 5 ml min until reaching a pressure of 150 bar At this point the pump delivers at a flow rate of 0 2 ml min until reaching a pressure of 250 bar The pump then delivers at a flow rate of 0 1 ml min until reaching a pressure of 345 bar then switches into its Keep pressure mode To test the pressure tightness of all individual components in the flow path of the pump itself use the leak test see Prep Pump Leak Test Description on page 98 Step 3 When the system pressure reaches 350 bar the preparative pump switches to its Keep pressure mode The flow ra
90. overs in place 110 Agilent 1260 Infinity Preparative Pump User Manual Maintenance 7 Cleaning the module The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth as liquid may drip into the module WARNING Liquid dripping into the electronic compartment of your module Liquid in the module electronics can cause shock hazard and damage the module gt Do not use an exessively damp cloth during cleaning gt Drain all solvent lines before opening any fittings Agilent 1260 Infinity Preparative Pump User Manual 111 7 Maintenance Overview of Maintenance and Repair Overview of Maintenance and Repair 112 Figure 23 on page 112 shows the main assemblies of the preparative pump The pump heads and its parts do require normal maintenance for example seal exchange and can be accessed from the front simple repairs Replacing internal parts will require to remove the module from its stack and to open the top cover Figure 23 Overview of Repair Procedures Agilent 1260 Infinity Preparative Pump User Manual Maintenance 7 22 33 44 55 66 71 88 99 1010 1111 Multi assembly with filters and pressure sensor at the rear see Exchanging the Filter Cup on page 120 Exchanging the Filter Frit on page 122 Leak sensor see Exchanging the Leak Sensorin the Serv
91. ower Consideration The module power supply has wideranging capability see Table 4 on page 27 It accepts any line voltage in the range described in the above mentioned table Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply WARNING Incorrect line voltage at the instrument Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage WARNING Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed 24 Agilent 1260 Infinity Preparative Pump User Manual CAUTION Site Requirements and Specifications 2 Unaccessable power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords
92. p 4 Setting Up the Pump Setting up the Preparative Pump Agilent ChemStation The Agilent 1260 Infinity Preparative Pump can be operated as Isocratic Pump or can be combined with an additional pump to form a gradient system Details for both pump configurations are explained in Flow Connections for a Single Isocratic Preparative Pump on page 42 for the isocratic pump and in Flow Connections for a Dual Binary Gradient Preparative Pump on page 45 for the gradient pump If the Agilent ChemStation software is started all pumps that are connected to the LC system are recognized and can be added to the list of configured modules see Figure 14 on page 57 If two pumps are configured they are treated as preparative gradient pump The first upper pump in the configuration will deliver solvent A and the second lower pump will deliver solvent B Configuration Available Modules Configured Modules fi G13298 S Autosampler SL IS offline EA G1361A Prep Pump G13614 1 Prep Pump DE10200112 G13614 2 Prep Pump a offline DE10200113 G13624 a G22584 RID Refractive Index Detector Dual Loop utosampler PS offline PP34300026 G13644 G13154 Automatic Fraction Collector DAD Diode Array Detector offline DE53500062 zl EEN Figure 14 List of Configured Modules Agilent 1260 Infinity Preparative Pump User Manual 57 4 58 Using the Preparative Pump Setting Up the
93. plot Typical failure modes are described below Please notice the difference between an error in the test and a failure of the test An error means that during the operation of the test there was an abnormal termination If a test failed this means that the results of the test were not within the specified limits Often it is only the damaged blank nut itself poorly shaped from overtightening that causes a failure of the test Before investigating on any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened Error Limits Exceeded for One or Both of the Encoder Values and One or Both of the Maximum allowed Flow Rates in the Constant Pressure Mode Probable cause Suggested actions 1 Air bubble Flush pump thoroughly 2 Loose or leaky fittings Ensure all fittings are tight or exchange capillary 3 Large leaks visible at the pump seals Exchange the pump seals 4 Large leaks visible at valve outlet valve Ensure the leaky components are installed filters multi assembly capillaries or EMPV tightly Exchange the component if required Run the EMPV cleaning procedure 100 Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 Maximum Allowed Flow Rate Exceeded for Both Pistons 1 and 2 Probable cause 1 Wrong solvent 2 Loose or leaky fittings 3 Contaminated EMPV 4 Loose pump head screws 5 Large leaks visible at t
94. pple can be monitored with one of these instruments 5 Starting with a compressibility setting of 50 x 10 bar increase the value in steps of 10 The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition 6 Repeat step 1 on page 67 through step 5 on page 67 for each preparative pump in a system with a multiple pump setup Agilent 1260 Infinity Preparative Pump User Manual 67 5 Optimizing Performance Table 11 Solvent Compressibility Solvent pure Compressibility 10 6 bar Acetone Acetonitrile Benzene Carbon tetrachloride Chloroform Cyclohexane Ethanol Ethyl acetate Heptane Hexane Isobutanol lsopropanol Methanol 1 Propanol Toluene THF Water 126 115 95 110 100 118 114 104 120 150 100 100 120 100 87 95 46 68 Agilent 1260 Infinity Preparative Pump User Manual Agilent 1260 Infinity Preparative Pump User Manual 6 Troubleshooting and Diagnostics Agilent Lab Advisor software 70 Overview of the Pump s Indicators and Test Functions 71 Status Indicators 72 Power Supply Indicator 72 Instrument Status Indicator 73 User Interfaces 74 Error Messages 75 Pressure Test 94 Prep Pump Pressure Test Description 94 Positioning the Blank Nut 95 Running the Pressure Test 96 Evaluating the Results 97 Leak Test 98 Prep Pump Leak Test Description 98 Running the Leak Test 99 Evaluating the Results 100 EMPV Cleaning
95. ptimal functionality of your flow cell Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cell If the flow cell is transported while temperatures are below 5 degree C it must be assured that the cell is filled with alcohol Aqueous solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add a small of organic solvents e g acetonitrile or methanol 5 Use of Solvents Observe the following recommendations on the use of solvents e Brown glass ware can avoid growth of algae e Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters e Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid and nitric acid especially at higher temperatures if your chromatography method allows replace by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCly Op gt 2COCly 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Agilent 1260
96. r cable are plugged in the correct positions before turning ON the seal wash pump 92 Seal Wash This error message is generated if the pressure sensor is incorectly plugged into the connector on the main board that should hold the seal wash motor cable Probable cause Suggested actions 1 Pressure sensor and seal wash pump Switch the connectors of the seal wash pump connector were connected to the wrong cable and the pressure sensor to meet the position on the main board identical correct positions P23 for seal wash cable P29 connectors allow to plug into the wrong for the pressure sensor cable from the multi positions by mistake assembly Wait for Encoder Position Timed Out Encoder position was not reached within specified time Probable cause Suggested actions 1 Flow too low Recalculate flow or wait time to allow to reach encoder position before encoder position time out 2 Pump OFF Turn ON pump Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 24V Supply for External Devices Low Voltage sensing of the 24 Volts CAN outlet shows low voltage probably fuse has blown Probable cause Suggested actions 1 Excessive current has been drawn by an Ensure that any device connected to the external device connected to the external 24 external 24 CAN DC out does not draw more CAN DC out than 100 mA as a permanent current or a maximum of 2 A for no longer than 2 seconds The error c
97. r of one of the modules 6 Press in the power switches to turn on the modules The Agilent control software e g ChemStation EZChrom OL etc can be also be connected to the system through a LAN cable which requires the installation of a LAN board For more information about connecting the control module or Agilent control software refer to the respective user manual For connecting the Agilent 1260 Infinity equipment to non Agilent 1260 Infinity equipment see Introduction to the Preparative Pump on page 8 Agilent 1260 Infinity Preparative Pump User Manual 41 3 Installing the Pump Flow Connections for a Single Isocratic Preparative Pump Tools required Parts required Preparations Wrench 1 4 5 16 inch for capillary connections Part number Description Other modules 1 G1361 68708 Parts from basis kit see Table 8 on page 32 Pump is installed in the HPLC system When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Danger of Explosion Pumping solvents at high flow rates generally results in electrostatic charging of the solvents which may result in the pot
98. reased to approximately 350 bar Then the EMPV is opened and the pressure is released very quickly The procedure is repeated 5 times in a sequence Agilent 1260 Infinity Preparative Pump User Manual 103 6 Troubleshooting and Diagnostics Running the Cleaning Procedure When When the EMPV is suspected of being leaky or contaminated with particles Tools required Wrench7 16 inch Parts required Part number Description 1 01080 83202 Blank nut Preparations Plug the pump outlet with a blank nut 01080 83202 at EMPV outlet Select EMPV Cleaning from the test selection menu 2 Start the procedure and follow the instructions Detailed instructions are provided in the Lab Advisor tool Run a leak test see Prep Pump Leak Test Description on page 98 after having performed the cleaning procedure to ensure tightness of the EMPV 104 Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 3 For better removal of any left over particles it will help to clean the ball of the valve with an additional procedure if the previous step did not help Using a 7 16 wrench remove deposits by turning the holding screw of the EMPV body back and forth about 1 4 wrench turn several times Figure 22 Removing Deposits from the EMPV with the Help of a Wrench 4 Remove the blank nut 5 Reconnect all capillaries Agilent 1260 Infinity Preparative Pump User Manual 105 6 Troubleshooting and Diagnos
99. refer to its documenation The following cards can be used with the Agilent 1260 Infinity modules Table 23 LAN Boards Type Vendor Supported networks G1369A Agilent Technologies Fast Ethernet Ethernet 802 3 RJ 45 G1369 60001 10 100Base TX recommended for re ordering J4106A Hewlett Packard Ethernet 802 3 RJ 45 10Base T J4105A Hewlett Packard Token Ring 802 5 DB9 RJ 45 10Base T J4100A Hewlett Packard Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX BNC 10Base2 These cards may be no longer orderable Minimum firmware of these Hewlett Packard JetDirect cards is A 05 05 Agilent 1260 Infinity Preparative Pump User Manual 157 9 Configuring the Preparative Pump Table 24 Recommended LAN cables Cross over network cable shielded 3 m long for point to point connection 5023 0203 Twisted pair network cable shielded 7 m long for hub connections 5023 0202 158 Agilent 1260 Infinity Preparative Pump User Manual Configuring the Preparative Pump 9 Interfaces Analog Signal Output The analog output connector s provide an analog signal output pressure signal The analogue data are digitalized by an A D converter and can then be distributed to a PC which is running with a non Agilent operating system that does not communicate via GPIB For details refer to the description of the main board of the module GPIB Interface The GPIB connector serves as the default interface bet
100. relay contacts The maximum settings are 30 V AC DC 250 mA fused BCD connector Board identification 12 Processor BCD interface register f Line driver RFI filter 7 250 mA External Ax External contacts i contact connector DRF filter Agilent 1260 Infinity Preparative Pump User Manual 155 9 Configuring the Preparative Pump There are general purpose cables available to connect the BCD output see BCD Cables in the Service Manual and the external outputs see External Contact Cable in the Service Manual to external devices Table 22 Detailed connector layout 1200 Pin Signal name BCD digit 1 BCD5 20 2 BCD7 80 3 BCD 6 40 4 BCD 4 10 5 BCD 0 1 6 BCD 3 8 7 BCD 2 4 8 BCD 1 2 9 Digital ground 10 BCD 11 800 11 BCD 10 400 12 BCD 9 200 13 BCD 8 100 15 5V Low 156 Agilent 1260 Infinity Preparative Pump User Manual Configuring the Preparative Pump 9 LAN Communication Interface Board The Agilent 1260 Infinity modules have one optional board slot that allows to add an interface board to the modules Some modules do not have this interface slot Refer to Interfaces on page 16 for details Description Part Number LAN Communication Interface Board G1369A G1369 60001 One board is required per Agilent 1200 stack It is recommended to add the LAN board to the detector with highest data rate For the configuration of the G1369A Lan Communication Interface card
101. replace capillary if necessary in channel 1 Maximum Allowed Flow Rate Exceeded for Piston 2 Probable cause 1 Externally leaking inlet or outlet valve in channel 2 2 Internally leaking inlet valve in channel 2 back flow 3 Internally leaking outlet valve in channel 1 back flow 4 Leaking seal or scratched plunger in channel 2 5 Loose or leaky fitting or capillary or fitting in channel 2 Suggested actions Ensure the valves are installed correctly Tighten the valves Clean or replace the inlet valve in channel 2 Clean or replace the outlet valve in channel 1 Exchange the pump seals in channel 2 Check the plunger for scratches Exchange if scratched Tighten fitting or replace capillary if necessary in channel 2 Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 EMPV Cleaning Prep Pump EMPV Cleaning Description Depending on the application particles can sometimes be collected in the EMPV valve This fast cleaning routine is designed to remove such particle deposits The routine should always be performed when the EMPV is suspected of being leaky or contaminated with particles Large internal leaks at the EMPV can easily be identified by observing a flow out through the waste tubing of the EMPV while the EMPV should be closed The EMPV outlet is plugged with a blank nut 01080 83202 After a short flushing routine the EMPV is closed and the pressure is inc
102. rious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal level are defined as e standard TTL levels 0 V is logic true 5 V is false e fan out is 10 e input load is 2 2 kOhm against 5 V and e output are open collector type inputs outputs wired or technique Agilent 1260 Infinity Preparative Pump User Manual Configuring the Preparative Pump 9 Table 25 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing preanalysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as pos
103. rits between the sensor pump head and pressure sensor at the rear of the multi assembly are free from blockage Clean the filters in the multi assembly 2 Blocked outlet ball valve Exchange the outlet ball valve 3 High friction partial mechanical blockage in Remove the pump head assembly Ensure there the drive assembly is no mechanical blockage of the pump head assembly or drive assembly 4 Defective drive assembly Exchange the drive assembly 5 Defective PPM board Exchange the PPM board Motor Direction The motor control has determined that the motor turns in opposite direction Probable cause Suggested actions 1 Cables connected in wrong orientation after Ensure the cables are correctly connected on a repair the main board and at the pump drive and reconnect with new orientation if necessary Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 Pump Off While Analysis This error message is generated if the pump was turned OFF during an analysis Power OFF or pump in a Not Ready condition Probable cause Suggested actions 1 Power OFF or pump in a Not Ready Turn ON the Pump condition Eliminate the cause of the Not Ready condition Index Missing The pump motion control expects one index signal for every turn of the drive wheel The error message is generated if this signal is not received within one complete turn of the drive wheel Probable cause Suggested
104. rtial mechanical blockage in the drive assembly 4 Defective drive assembly 5 Defective PPM board Agilent 1260 Infinity Preparative Pump User Manual Suggested actions Ensure the capillaries and frits between the pump head and pressure sensor at the rear of the multi assembly are free from blockage Clean the filters in the multi assembly Exchange the outlet ball valve Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or drive assembly Exchange the drive assembly Exchange the PPM board 85 86 Troubleshooting and Diagnostics Motor Maximum Power Fault The motor control has determined that the set maximum for the motor power was exceeded for longer than 1 second To protect the circuitry from damage this error event is issued in this case Blockages in the flowpath are usually detected by the pressure sensor in the multi assembly which results in the preparative pump switching OFF when the upper pressure limit is exceeded If a blockage occurs before the pressure sensor i e the pressure increase cannot be detected by the pressure sensor the preparative pump will continue to pump As pressure increases the pump drive draws more current When the current reaches the maximum limit the preparative pump is switched OFF and the error message is generated Probable cause Suggested actions 1 Flow path blockage in front of the pressure Ensure the capillaries and f
105. se of an instrument error Seal Wash Pump The settings for seal wash pump can be controlled in this section The seal wash can be switched off or a single wash can be started for a user specified time In addition you can choose to turn the seal wash on periodically for a user defined on time The on time must be between 0 1 min and 70 of the entire period Running the seal wash periodically will reduce the ware of the pump seals and increase the maintenance interval of your pump For more details read the chapter How to Configure the Seal Wash Function on page 66 Control A Instrument 1 DE10200112 f x m Pump Purge Error method C On C On Off J Take current method Off fof 0 00 rain r Seal Wash Pump C Off C SingleWash Duration 10 min Periodic Period 1 0 min 0 2 min ON time m Automatic Turm On J Tumpumponat Date 8 10 2007 Time J 15 55 41 hh mm ss OK Cancel Help m d yyyy Figure 20 Control dialog box The Control dialog box is also available for channel B on gradient pump systems To prevent the pump from running dry you can specify the fillings of your solvent reservoir in the Solvent Bottle Filling dialog box Figure 21 on page 63 This dialog box can be opened through Instrument gt More Preparative pump gt Bottle Filling or by clicking on the bottle icon in the graphical user interface Figure 14 on page 57 Agilent 1260 Infinity Preparativ
106. sible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Agilent 1260 Infinity Preparative Pump User Manual 161 9 162 Configuring the Preparative Pump RS 232C The RS 232C connector is used to control the instrument from a computer through RS 232C connection using the appropriate software The RS 232C interface can be used for controlling the module with PCs running non Agilent Control Software Most but not all of the Control Software products of other manufacturers communicate via this RS 232C interface If in such a scenario the RS 232C interface cannot be used the analog output connector can be used instead in which case an additional handheld controller connected via the CAN Connector must be used The RS 232C connector can be configured with the configuration switch module next to the GPIB connector The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table 26 RS 232C Connection Table continued Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Agilent 1260 Infinity Preparative Pump User Manual DCD RX TX DTR GND DSR RTS CTS RI DB9 DB9 Figure 37 RS 232
107. solutions Under normal conditions the default settings reduce the pressure pulsation to values below 2 of system pressure for a complete system with a preparative scale sized column installed that will be sufficient for most applications If the compressibility values for the solvents used differ from the default settings it is recommended to change the compressibility values accordingly Compressibility settings can be optimized by using the values for various solvents described in Table 11 on page 68 If the solvent in use is not listed in the compressibility table when using premixed solvents and if the default settings are not sufficient for your application the following procedure can be used to optimize the compressibility settings Use the preparative pump with a flow rate of at least 20 ml min 1 Start the preparative pump with the adequate flow rate The system pressure should be between 50 and 150 bar 2 Before starting the optimization procedure the flow must be stable Before starting the procedure check the tightness of the system with the help of the built in pressure test see Prep Pump Pressure Test Description on page 94 3 Verify the tightness of the pump itself piston seal combinations valves filters fittings etc with the help of the built in Leak Test see Prep Pump Leak Test Description on page 98 4 Your pump must be connected to a Agilent ChemStation or handheld controller the pressure and ri
108. stics 6 Running the Leak Test When When problems with leaks are suspected or after maintenance of flow path components e g pump seals filters EMPV or capillaries to prove pressure tightness up to 350 bar Tools required inch wrench Parts required Part number Description 1 01080 83202 Blank nut 500 ml lsopropanol Preparations Thoroughly flush the pump with isopropanol and remove all contaminations and potential air bubbles release the pressure in the system and position the blank nut Make absolutely sure that all parts of the flow path that are part of the test are very thoroughly flushed with IPA before starting to pressurize the pump Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail It is recommended to run the EMPV cleaning procedure see Prep Pump EMPV Cleaning Description on page 103 prior to running the Leak test to remove any potential deposits from the EMPV valve 1 Select the leak test from the test selection menu 2 Start the test and follow the instructions Detailed instructions are provided in the LMD Tool Evaluating the Results on page 100 describes the evaluation and interpretation of the leak test results Agilent 1260 Infinity Preparative Pump User Manual 99 6 Troubleshooting and Diagnostics Evaluating the Results Defective or leaky components in the pump head lead to changes in the leak test pressure
109. sumption is 220 VA There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply The security lever at the power input socket prevents the module cover from being taken off when line power is still connected Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1260 Infinity Preparative Pump User Manual Interface BCD board slot Remote oooooong ff RS232C CAN cable to previous module CAN bus Introduction to the Preparative Pump Electrical Connections 0000000 jy 8 bit configu ration switch CAN DC out Figure 3 Rear View of Preparative Pump Electrical Connections and Label Agilent 1260 Infinity Preparative Pump User Manual 1 15 1 Introduction to the Preparative Pump Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table 2 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN GPIB RS 232 Analog APG Special optional on board Remote Pumps G1310A ISO 2 Yes No Yes Yes 1 Yes G1311A QUAT G1312A BIN G2226A NANO G1312B BIN SL 2 Yes No Yes Yes 1 Yes G1361A PREP 2 Yes No No Yes No Yes CAN DC OUT for CAN slaves Samplers G1313A STD 2 Yes No Y
110. t 1260 Infinity Preparative Pump User Manual Maintenance Simple Repairs 7 1 Disconnect the capillaries at the outlet valves and the tubes at the inlet valves Beware of leaking solvents Remove seal wash tubes Outlet capillaries T 2 Using a 4 mm hexagonal key step wise loosen and remove the four pump head screws and remove the pump head from the pump drive Pump head screws TA T lt h am i E z Lanny A q P ae i es tO ty Y 7 L j ry lj f n N 3 Place the pump head on a flat surface facedown Pull out the plungers from the spring assembly Plungers T l Spring assembly io 4 Loosen the two screws on the spring assembly and carefully pull the spring assembly and pumphead apart Screws Spring assembly Nc Hold here s Pump head Agilent 1260 Infinity Preparative Pump User Manual 127 7 Maintenance 5 Remove secondary seals between the pumphead and the spring assembly by using the plungers Secondary seals 128 Agilent 1260 Infinity Preparative Pump User Manual Maintenance 7 Reassembling the Pump Head Assembly Tools required Screwdriver Pozidrive 1 4 mm hexagonal key 10 mm wrench Preparations Clean all parts before reassembly 1 Place front pump head facedown on a flat surface Insert new seals with the spring facing the pump body Press the seals firmly into place Make sure not
111. t valve and remove the valve adapter including the valve assembly from the pump head Figure 24 Ball Valve Parts Agilent 1260 Infinity Preparative Pump User Manual 117 7 Maintenance When Tools required Parts required 118 Removing an Outlet Ball Valve Top of the Pump Head If internally externally leaking back flow Wrench 17 mm Wrench 1 4 inch Part number Description 1 G1361 60012 Valve assembly The outlet valves do not necessarily have to be replaced in pairs Use the built in Leak Test see Prep Pump Leak Test Description on page 98 to identify the valve that is not working properly 1 Disconnect the solvent outlet capillary at the valve adapter with a 1 4 inch wrench Beware of leaking solvents 2 Using a 17 mm wrench loosen the outlet valve adapter and remove the valve adapter from pump head 3 Lift the valve adapter out of the housing in the pumphead 4 With a pair of tweezers carefully remove valve assembly Replacing an Inlet Outlet Valve 1 Before inserting the new valve assembly clean the valve adapter by placing it into an ultrasonic bath if it shows visible signs of contamination Clean the valve housing in the pumphead by filling a syringe with alcohol and flushing the housing thoroughly 2 Insert a new valve assembly into the valve adapter The arrow on Figure 24 on page 117 indicates the flow direction Make sure the valve assembly is fully inserted into the valve adapter
112. te to compensate for leaks should from this point onwards be no more than 10 ul minute 94 Agilent 1260 Infinity Preparative Pump User Manual Troubleshooting and Diagnostics 6 Positioning the Blank Nut If a specific component is suspected of causing a system leak place the blank nut immediately before the suspected component then run the pressure test again If the test passes the defective component is located after the blank nut Confirm the diagnosis by placing the blank nut immediately after the suspected component The diagnosis is confirmed if the test fails Agilent 1260 Infinity Preparative Pump User Manual 95 6 Troubleshooting and Diagnostics Running the Pressure Test When If problems with small leaks are suspected After maintenance of flow path components e g pump seals injection seal to prove pressure tightness up to 350 bar Tools required inch wrench Parts required Part number Description 1 01080 83202 Blank nut 500 ml lsopropanol Preparations Thoroughly flush the entire system with Isopropanol and remove all contaminations and potential air bubbles release the pressure in the system and position the blank nut Make absolutely sure that all parts of the flow path that are part of the test are very thoroughly flushed with IPA before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail 1 Select the
113. ten agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in dama
114. ter Cup on page 120 and then the filter frit after having pumped another 200 ml of solvent Changing the Filter Frit When When the back pressure exceeds 10 bar pumping 50 ml min of pure water with open purge valve After 200 ml solvent have been pumped through the pump after a filter cup exchange Tools required Wrench 7 16 inch Wrench 1 4 inch Hex key 4mm Wash bottle containing Isopropanol Parts required Part number Description 1 5022 2192 Filter frit 2 um 1 Using a 1 4 inch wrench disconnect the capillary between multi assembly and EMPV 122 Agilent 1260 Infinity Preparative Pump User Manual Maintenance 7 2 Insert a hex key in to hole in the multi assembly Hold the hex key firmly and unscrew the locknut using the 7 16 inch wrench Filter frit Filter holder Figure 26 Changing the Filter Frit 3 Pull out the filter holder and filter 4 Flush the filter port and filter holder using a wash bottle containing isopropanol Agilent 1260 Infinity Preparative Pump User Manual 123 7 Maintenance 5 Install a new filter frit into the filter holder with the collar of the filter frit facing towards the multi assembly Reconnect the filter holder into multi assembly Finger tighten screw and then turn 1 8 turn using 7 16 inch wrench and hex key Filter holder Filter frit Figure 27 Changing the Filter Frit 6 Reconnect capillary between multi assembly and EMPV using a 1 4 inch wrench 124
115. ternal contacts BCD LAN and so on For identification and location of the connectors see the module manual Agilent 1260 Infinity Preparative Pump User Manual 17 1 18 Introduction to the Preparative Pump Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement If a Agilent 1260 Infinity detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler LAN The 1200 Infinity modules have either an interface slot for an LAN card e g Agilent G1369A LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a connected PC with the appropriate control software e g Agilent ChemStation GPIB This interface is not available in all modules and may be removed from the modules in future The GPIB connector is used to connect the module with a computer The address and control switches next to the GPIB connector determine the GPIB address of your module The switches are preset to a default address and recogn
116. the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed Sharp metal edges Sharp edged parts of the equipment may cause injuries gt To prevent personal injury be careful when getting in contact with sharp metal areas When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap when handlingelectronic boards and components Agilent 1260 Infinity Preparative Pump User Manual 109 7 Maintenance The electronics of the preparative pump will not allow operation of the preparative pump when the top cover and the top foam are removed A safety light switch ON the main board will inhibit the operation of the preparative pump Always operate the preparative pump with the top foam and top c
117. tics EMPV Cleaning 106 Agilent 1260 Infinity Preparative Pump User Manual Agilent 1260 Infinity Preparative Pump User Manual 7 Maintenance Introduction to Maintenance and Repair 108 Simple Repairs 108 Warnings and Cautions 109 Cleaning the module 111 Overview of Maintenance and Repair 112 Simple Repairs 114 Checking and Cleaning the Solvent Inlet Filters 115 Exchanging the Inlet Outlet Ball Valves 117 Exchanging the Filter Cup 120 Exchanging the Filter Frit 122 Exchanging the Seal Wash Pump Cartridge 125 Removing and Disassembling the Pump Head Assembly 126 Reassembling the Pump Head Assembly 129 Exchanging the Optional Interface Board 131 Replacing the Module s Firmware 132 ee Agilent Technologies 107 7 Maintenance Introduction to Maintenance and Repair Simple Repairs The module is designed for easy repair The most frequent repairs such as plunger seal change and purge valve frit change can be done from the front of the module with the module in place in the system stack These repairs are described in Simple Repairs on page 114 108 Agilent 1260 Infinity Preparative Pump User Manual Maintenance 7 Warnings and Cautions Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access
118. to system G1361 67302 1 Bottle AMBER 2 0 I 9301 6341 1 Wrench open end 14 17 mm 8710 2435 1 Wrench open end 1 4 5 16 8710 0510 2 Wrench open end 7 16 3 8 8710 0972 1 Hex key 3 mm 8710 2411 1 Hex key 4 mm 8710 2392 1 Pair of tweezers 5022 2195 1 Priming Syringe re order no pck of 10 5062 8534 1 Adapter for Priming Syringe 9301 1337 1 Tubing for Priming Syringe G1361 87300 1 Spare bottle inlet filter frit 3150 0944 1 Spare frit adapter for 4 7 mm OD tubing G1361 23205 1 Glass stop valve for stopped pumps 5042 6464 1 Spare filter cup 3150 0942 1 Spare SST frit 2 um 5022 2192 1 CAN cable 1m 5181 1519 1 Agilent 1260 Infinity Preparative Pump User Manual 145 8 Parts and Materials for Maintenance Table 19 Basis Kit Contents G1361 68708 shipped with the first pump of a system Description Part Number Quantity Union preparative 5022 2133 1 Sanding paper no PN 2 146 Agilent 1260 Infinity Preparative Pump User Manual Parts and Materials for Maintenance 8 Preparative Pump Gradient Kit Table 20 Gradient Kit Contents G1361 68707 shipped with every additional pump ina gradient system Description Part Number Quantity Bottle head assembly G1361 60022 1 Tube seal wash 0890 1764 1 Waste tube 5042 2461 1 Bottle clear 2 01 9301 6342 1 Capillary 5 pump B to T union G1361 67304 1 Capillary 6 T union to inlet of mixer G1361 67305 1 Female T union 0100 1016 1 Mixer 79835 87330 1 Capillary 7 pump A to T union G1361
119. tronic fuse that will switch OFF the supply voltage This prevents the damage of components Onboard Battery An onboard lithium battery buffers the electronic memories when the preparative pump is turned off For safety information on lithium batteries see Lithium Batteries Information on page 173 Agilent 1260 Infinity Preparative Pump User Manual 151 9 Configuring the Preparative Pump Block Diagrams to A D Converter Encoder see next page Optocoupler Pump head Motor DriveJ 34 Identification tag Index EEPROM SS Transceiver TAG __sJ lt gt y to A D Converter see next page FPGA Seal wash pump Drive Oo ee Processor EMPV el magn purge valve Valve drive CAN DC OUT ON OFF CAN DC OUT AN G to ADC see next page Figure 34 Block Diagram Pump Controller Board 152 Agilent 1260 Infinity Preparative Pump User Manual Configuring the Preparative Pump 9 see previous page Vii A D converter Multiplexer ae _ Pressure sensor ASIC Processor Amplifier amp ambient meas lt Leak sensor Drive Fan Figure 35 Block Diagram Pump Controller Board Agilent 1260 Infinity Preparative Pump User Manual Control Signal lt gt Data Address lt gt D
120. ttom SST 1 16 o d 0 6 mm i d 178 mm long Prep Capillary 5 gradient kit Union to EMPV2 SST 1 16 o d 0 6 mm i d 111 mm long Prep Capillary 6 gradient kit Union to mixer SST 1 16 o d 0 6 mm i d 40 mm long G1361 60022 5042 6404 5042 6405 5062 2463 G1361 67302 G1361 67303 5062 2461 5062 2463 0890 1764 0890 1764 5042 6422 0890 1764 G1361 67300 G1361 67301 G1361 67304 G1361 67305 Agilent 1260 Infinity Preparative Pump User Manual Parts and Materials for Maintenance Table 18 Hydraulic Path Item Description Part Number 17 Prep Capillary 7 gradient kit EMPV1 to union SST 1 16 o d 0 6 mm i d 367 mm long 18 Union gradient kit Fitting FRONT Fitting BACK Fitting 1 16 re order 10 pk G1361 67306 0100 1016 5062 2418 Agilent 1260 Infinity Preparative Pump User Manual 143 8 Parts and Materials for Maintenance Hydraulic Path fd T 1 i 3 UT ih T i i Jo ee i in a ml 5a 10 gt vyp ABs LH 144 Agilent 1260 Infinity Preparative Pump User Manual Parts and Materials for Maintenance 8 Preparative Pump Basis Kit This kit is part of the G1361A Preparative Pump Table 19 Basis Kit Contents G1361 68708 shipped with the first pump of a system Description Part Number Quantity Bottle head assembly G1361 60022 1 Tube seal wash 2 m 0890 1764 1 Waste tube reorder No 5062 2461 1 Capillary 3 pump outlet
121. ual Introduction to the Preparative Pump 1 Early Maintenance Feedback EMF The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user settable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled For details on EMF counters and how to use them see Agilent Lab Advisor Agilent 1260 Infinity Preparative Pump User Manual 11 1 Introduction to the Preparative Pump Instrument Layout 12 The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations e the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields
122. waste G1361 67305 Mixer G1361 67304 Seal wash pump to pump head EMPV to waste 0890 0615 From seal wash solvent bottle to seal wash pump Waste outlet corrugated waste tubing Mixer out to next module G1361 67302 Figure 13 Flow connections to the preparative pump 48 Agilent 1260 Infinity Preparative Pump User Manual Installing the Pump 3 Get the System Ready for the First Analysis When you are using the system for the first time it is recommended to prime it to remove all the air and the possible contamination introduced in the flow path during the installation Priming your preparative LC system with the pump The pump should never be used for priming empty tubings never let the pump run dry Use a syringe to draw enough solvent for completely filling the tubings to the pump inlet before continuing to prime with the pump WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1 Set the flow rate to 5 ml min and pump for 5 minutes with an open EMPV to prime your new tubings and capillaries and to remove potential air bubbles or contamination
123. ween an Agilent 1260 Infinity module and the Agilent Control Software The GPIB connector is used to connect the module with a computer The address and control switches next to the GPIB connector determine the GPIB address of your module The switches are preset to a default address and recognized by the operating software from Agilent Technologies CAN Interface The CAN connectors serve as the interface between the Agilent 1260 Infinity modules themselves All modules in the Stack must be interconnected It is a 2 wire serial bus system supporting high speed data communication and real time requirements Agilent 1260 Infinity Preparative Pump User Manual 159 9 160 Configuring the Preparative Pump APG Remote Interface The APG Remote connector serves as the interface between the Agilent 1260 Infinity and other Agilent APG remote compatible products It may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a se
124. ydraulic Path Overview The pump flow leaving the pump head passes through the multi assembly which combines the flow of the two pistons filters the solvent and contains a pressure sensor to monitor system pressure No hydraulic damper is included in the system This makes very fast gradients possible From the multi assembly the flow is routed to the purge valve and from there either directly into waste or on to the next module normally injection valve or sampling device Gradients are formed with two combined pumps as high pressure gradients The combined flow is flowing through a passive mixer to smooth composition With its high pressure mixing principle gradient systems and the usually high flow rates no degassing of solvents is necessary To avoid outgassing in the detector cell a back pressure regulating device is recommended for applications that demand it Inlet valve Inlet valve 2 e e From solvent bottle in binary gradient system Female T only in binary gradient system Mixer E in binary gradient system From Pump B only Seals 2x2 Piston 1 Multi Assembly with 2 filters and pressure sensor Piston 2 Figure 2 Schematics of the Preparative Pump Agilent 1260 Infinity Preparative Pump User Manual 9 1 10 Introduction to the Preparative Pump Table 1 Preparative Pump Details Materials in contact with mobile phase Bottle head assembly glass frits PTFE

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