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1. coordinates the geometrical parameter of the sample will be computed J ATON Aaa ERF ZAR A GSE TEATE i gn BH WHE JAE E EE DAA 6 Reconstruction In the following pictorial diagram the reconstruction is conducted according to the following parameters Platform CPU version or GPU version Series of original image original image lf the module called public parameters are set the content is acquired automatically Series of images after reconstruction set the name of the image after reconstruction Initial models The user can select the backprojection assigning a value of 0 random values weighted backprojection or existing reconstructed image weighted backprojection is not realized yet Reconstruction algorithm ART SIRT or WBP WBP is not realized yet Iteration the number of times of reconstruction Relaxation factor the relaxation factor of reconstruction BEER PSR FE 5 _RF2multsqznormp_aligned_ Re Res mrc 8 Lac PERVAN ELIS HM 8 Ac TH WHEN JAHRE ae If the user select the existing reconstructed image in the initial models series of reconstructed image are chosen ae psa ARIES Fa HEEJ The pionne are advanced parameters Geometrical parameter they are required by the module of determining geometrical parameter Angle parameter they are set from the module of public parameters by default The path of light float or 0 1 Range f
2. establishing model fast If original image and the file of angle parameters are set in the module called public parameters original image and the file of angle parameters are acquired automatically Setting the thickness The height of the object reconstructed will be required according to the thickness and the file of angle parameters Three lines are drawed separately in the the upper lower middle parts which are bound with blue dotted lines after inputting parameters that is to say corresponding lines are drawed just you click the corresponding point of position which is used to fix the position in which the An slice is reconstructed then you click the button marked Jf R in order to make the programme run Aeri BE fs iM 400s AESH an_debug angle par Ab SHES PLA os Fh RISE enggy Testjiemian debug S RF2multsqznormp aligned mrc __ ap ae i fH Hae JL fe Sl ta PLE HR He Fy JA AES HES ea eee 5 Determining geometrical parameter In the last step three images will be gotten so you can select different images by clicking the button marked E 5 or F 3K In the every image you can move four controlling balls of two lines to fix the bound of the image reconstructed Running the programme by clicking the button marked FF 4R with view to getting the coordinates of four points when the process of three images is finished the coordinates of twelve points will be required According to those
3. ATOM Software Usage Manuals Installation guide To run the program you type a command line of either atom_install bin According to clew input the installing path that can be absolute or relative In the last catalogue should not be added If you directly input the enter the installation is conducted in the default directory Please check the authority of writing to the installation directory Waiting for the moment in which installation is successful ATOM path will be gotten under the specified path of user at the same time the software will be installed under the directory If the user uses tcsh or csh please run the command source cshrc to update environment variable If the user uses other shells please set the environment variable PATH or LD_LIBRARY_PATH by yourself and insert their content in to the ATOM catalogue If the path you inputted is bin when installing the programme bin ATOM is necessary to be added in the environment variable The installation was successful Please run the ATOM to come to use 1 Different linguistic choices in Chinese or English The following figure is shown Ye ChooseLanguage English 2 Public parameters Parameter lists Original image stack of original images measured Angle parameter rotation angles in the process of measure Angstrom Pixel the amount of angstrom per one pixel when you have finished inputting three parameters click the pr
4. esentation PAR Fie F Pec HERTHA HAE LASS 8 lac EH WHE TEE Se 3 2D Image matching a aron C aon ERASER ug s RF2mutsgzrormp signede O RERLAE AE LATS RH 8 Dac BH Fe Fai HEIs B WHE JARRE ae Iteration times are twice by default at least once You can input the iteration times or adjust them by the right arrow Series of original image are selected by clicking the button marked in addition you can directly input the path If the original images are selected in the module called public parameters related address is filled automatically but the address still may be modified by yourself at the same time all selected images are seen by clicking the button marked E Series of matched images whose default filename is the name of original image followed by aligned are results that images are matched which can be modified or inputted by yourself or skimmed by clicking the button marked If the computing of rotation parameter is necessary when the computing of 2D Image matching is conducted choose the radio button When the computing is finished the button marked 2 4 is effective When you click the button marked Jf 4n the validity of parameters will be checked If parameters are effective the programme will run and the output will be shown in the following textbox meanwhile the reminder will be also shown when the computing is finished 4
5. ull reconstruction or part reconstruction Weight 0 unweighed 1 weighed BSR FSR FE ZER Ac aia 2 RD HAE LATS HH 8 Ac FH zhanggy Testjiemian_debug angle par WHEN JAE ae Note Prerequisites Public parameters are interactive with the corresponding parameters of other processes that is after submitting the public parameters the correspoding parameters will be modified and the display of public parameters will be also changed While when the input mode instead of navigation mode is used to change the corresponding parameters of other processes the parameters will not be changed at the same time
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