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PHD 22/2000 Syringe Pump Series User`s Manual

1. Standard End see manual for loading syringes Push Pull Mechanism Moveable Syringe Holder and Clamp all syringes mount on this holder 1 to 30 ml only see figure below Posterior Syringe Clamp Pulley Cover 2400 414 2 2400 411 Thumb Nut Syringe Clamy 5103 050 2408 Stop Plate Syringe Holder moveable 2400 418 2400 412 Remove this piece when using large syringes Set Screw 2 5115 015 Ass y Pusher 2400 410 T Knob 5100 055 Anti Syphon P P 2400 420 T Knob 5100 055 Figure 4 Syringe Loading Infuse Withdraw or Programmable model shown Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Operation 7 Locate Allen Wrench 7 in its holder on the back of the Syringe Holder Block and remove Loosen front and rear Infusion Adjustable Stops 8 right side and slide them both to the left until they contact the retaining bracket 4 Using the Allen Wrench tighten both Infusion Adjustable Stops 8 8 Squeeze the Release Tabs 1 and move the Pusher Block 2 to the left until the syringe plunger is located as far out of the syringe as intended to travel Then squeeze the Release Tabs 1 and move the Pusher Block 2 to the right just enough so that the end of the syringe plunger is just short of the maximum trav el allowable and release the Release Tabs 1 This step is not required for the PHD 22 2000 Infusi
2. Syringe Refill lt gt OPI i lt _ Syringe _ q Cl oT q D o Figure 16 Pump to Valve Tubing Connection Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Appendix K Ordering Information Harvard PHD 22 2000 Syringe Pump Series Pump Versions Standard 50 Ibs Force Regular Remote High Power 66 Ibs Force Regular Remote PHD 22 2000 Infusion Only PHD 22 2000 Infuse Withdraw PHD 22 2000 Programmable Infuse Withdraw PHD 22 2000 Infusion Only with 6 10 Multirack PHD 22 2000 Infusion Only with 4 x 140 Multirack PHD 22 2000 Infusion Only with Microliter Rack PHD 22 2000 Infuse Withdraw with 6 10 Multirack PHD 22 2000 Infuse Withdraw w 4 x 140 Multirack PHD 22 2000 Infuse Withdraw with Microliter Rack PHD 22 2000 Programmable with 6 10 Multirack PHD 22 2000 Programmable with 4 x 140 Multirack PHD 22 2000 Programmable with Microliter Rack MA1 70 2000 MA1 70 2001 MA1 70 2002 MA1 70 2003 MA1 70 2004 MA1 70 2005 MA1 70 2006 MA1 70 2007 MA1 70 2008 MA1 70 2009 MA1 70 2010 MA1 70 2011 MA1 70 2100 MA1 70 2101 MA1 70 2102 MA1 70 2103 MA1 70 2104 MA1 70 2105 MA1 70 2106 MA1 70 2107 MA1 70 2108 MA1 70 2109 MA1 70 2110 MA1 70 2111 Harvard PHD 22 2000 Push Pull Syringe Pump Series Standard 50 Ibs Force Pump Versions PHD 22 2000 Infusion Withdraw Regular MA1
3. The data value entered can be reviewed as described above Note Certain data items have multiple settings For these after the ENTER key is pressed the display will prompt you for the additional information Various rules apply to when what and how data can be set at various times See the relevant section for further details SPECIAL FEATURES ACCESS TABLE Pressing the green outlined SET key and then the appropriate numerical key will allow the setting of several special features Pressing the appropri ate numerical key again will scroll between the options provided Refer to the table below When the desired option is displayed press the green out lined ENTER key RS 232 Protocol SET 1 Alarm Mode SET Power Up Mode SET 3 Publication 5400 008 REV F 3 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual User Interface Entering Data Step Key Setting RS 232 Protocol Description 1 SET SET WHAT Press and release SET key Pump Mode 2 MODEL 44 PROTOCOL Press and release 1 key Pump Mode Current RS 232 protocol displayed 3 1 MODEL 22 PROTOCOL Press and release 1 key to Pump Mode change RS 232 protocol 4 XXXXX ml INFUSE Press ENTER to accept Pump Mode return to main display Step Key Setting Alarm Mode Description 1 SET SET WHAT Press and release SET key Pump Mode 2 2 ALARM VISUAL ONLY Press and release 2 key Pump Mode Current alarm mode displayed 3 2 ALARM VISUAL amp AUDIBLE Press and release 2 key to
4. min seconds Figure 9 Periodic Dispense Loop This is an example of a series of periodic dis penses of varying volumes and intervals For this application Strategy 2 dispenses are used Note that between the third and fourth dis penses is a 45 minute interval Each dispense in the first sequence is separated by a pause inter val of 1 30 Since after the third dispense there already will be a 1 30 pause an additional pause of 43 30 is used to extend the pause to the desired 45 00 Sequence 5 is a RESTART command causing the series of dispenses to be continuously repeated until the pump is stopped Publication 5400 008 REV F PROGRAM PRINTOUT PROG1 SEQ 1 DISPENSE 15 000 ml mn 3 5000 ml 0 01 30 INTERVAL 3 REPEAT INFUSE PROG1 SEQ 2 PAUSE 0 43 30 INTERVAL PROG1 SEQ 3 DISPENSE 25 700 mli mn 6 7500 ml 0 05 00 INTERVAL 2 REPEAT INFUSE PROG1 SEQ 4 DISPENSE 20 000 ml mn 4 3000 ml 0 02 30 INTERVAL 4 REPEAT INFUSE PROG1 SEQ 5 RESTART Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Programming Tutorial Combination of Infusion and Withdraw Profiles Example 100 90 80 70 60 50 40 RATE ml min 30 20 10 Figure 10 Combination Infusion and SEQUENCE 4 5 6 TIME seconds Withdraw Profiles 8 Here is an example of a more com plex profile program Each run of the infusion
5. or time interval remaining in an operation expressed with 5 digits plus a decimal point Next is the units of the previous number expressed as ml for milli liters g for grams if a scale is attached or time for a time interval Third is the pump ing direction either INFUSE or REFILL or the current operating state of the pump A diamond symbol in the last character of the display indicates pump chain commu nication has been received Setting displays are used to facilitate entering control information and data into the pump Data entry will be discussed in detail later in this section Informational messages occur at various times to indicate such items as a data setting out of range or a detected problem such as the pump stalling Pressing any key clears the message from the display The second line of the display is also divided into 3 sections The first is the Running Mode Pump Vol Prog The second section is the AutoFill indication no message if Auto Fill is Off If On AutoFill ON is displayed The third section is a pair of direction al arrow heads lt or gt indicating that the pump is running and in what direction The keys are used for entering control information and data into the pump These keys are grouped into 3 sections see Figure 1 Set keys Toggle keys and Data Entry keys Publication 5400 008 REV F 5 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual User Int
6. s inch tube fitting MA1 70 2268 8 ml Stainless Steel Syringe with inch tube fitting MA1 70 2269 2 5 ml Stainless Steel Syringe with 1s inch tube fitting MA1 70 2246 1 Replacement Tip with O Ring for 8 ml or 2 5 ml Syringe MA1 70 2245 Replacement Tip with O Ring for 8 ml Syringe MA1 5013 087 Replacement Syringe O Ring Chemraz 8 ml Syringe MA1 5013 088 Replacement Syringe Back Up O Ring Teflon 8 ml Syringe MA1 5013 110 Replacement Tip O Ring Chemraz MA1 70 2271 Repalcement Bal Seal for 2 5 ml Syringe Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Appendix C Min Max Flow Rates Minimum flow rates are taken from the smallest inside diameters and maximum flow rates are taken from the largest inside diameters of the syringes supplied by the eleven most widely used syringe manufacturers Nominal Minimum Maximum Flow Rates for Various Syringes Actual Limits will vary depending on manufacturer Syringe pl hr ml min i Min 0 5 pl 0 0001 95 330 tpl 10 0002 190 70 2ul 10 0004 381 30 Sul 0 0010 953 17 10ul 10 0019 1 9013 25ul 0 0046 4 7752 50ul 10 0092 9 5511 100 pl 0 0183 19 153 250 pl 10 0454 47 532 500 pl 0 0911 95 492 1000 pi 0 0031 190 95 imi 0 0033 205 30 2ml 0 0119 747 35 2 5 ml 0 0076 476 21 3 ml 0 0100 11 231 5 ml 0 0208 21 781 10 ml 0 0301 31 486 20 mi 0 0523 54 804 30 ml 0 0673 70 518 50 ml 0 1019 106 76 100 0 1740 182 40 140 ml 0 21
7. 10 100 1 46 60 29 10 250 2 30 Becton Dickinson 500 3 26 i Plastic f Sherwood Monoject imi Aetna Plasti pak Plastic 25 7 28 Size Diameter Size Diameter 5 10 30 10 14 57 1cc 4 78 mm 1 66 4 65 mm 3 8 66 3 8 94 E 5 12 06 6 12 70 Hamilton Microliter 10 14 50 42 15 90 Series Gastight 20 19 13 20 20 40 Size Diameter 30 21 70 35 23 80 0 5 pl 0 103 mm 50 60 26 70 60 26 60 0 1457 140 38 40 Air Tite ee All Plastic 0 460 Cadence Science Inc i Size Diameter Formly Popper amp Sons Inc 0 729 9 60 mm Perfektum Glass 1 031 12 45 1 46 15 90 22 50 0 5 3 45 4 61 mm 29 00 4 50 2 5 7 28 8 92 5 10 3 Unimetrics 8 99 10 14 57 Series 4000 amp 11 70 25 23 0 5000 ae 50 32 6 i 19 58 Size Diameter 22 70 Size Diameter 23 Becton Dickinson 0 460 mm 29 00 Glass All Types 0 729 35 70 1 031 Size Diameter 1 460 Renfac 0 5ul 4 64 mm 2 300 Diameter 1 4 64 3 260 eee 2 5 8 66 4 610 orem 5 11 86 rae 10 14 34 14 55 f 23 20 30 22 70 27 60 50 28 60 100 34 90 Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Appendix B Stainless Steel Syringes Harvard High Pressure Stainless Steel Syringes CatNo Product Replacement Parts MA1 5013 089 Chemraz O Ring 20 ml MA1 5013 090 Chemraz O Ring 50 ml MA1 5013 091 Chemraz O Ring 100 ml MA1 5013 092 Chemraz O Ring 200 ml MA1 5013 109 Chemraz Tip Seal O Ring sizes
8. 70 2020 PHD 22 2000 Programmable Infuse Withdraw MA1 70 2019 Harvard PHD 22 2000 HVP High Volume and Pressure Standard over 400 Ibs Force Regular Pump Versions PHD 22 2000 HVP High Volume and Pressure MA1 70 2023 Publication 5400 008 REV F MA1 71 2000 MA1 71 2001 MA1 71 2002 MA1 71 2003 MA1 71 2004 MA1 71 2005 MA1 71 2006 MA1 71 2007 MA1 71 2008 MA1 71 2009 MA1 71 2010 MA1 71 2011 MA1 71 2100 MA1 71 2101 MA1 71 2102 MA1 71 2103 MA1 71 2104 MA1 71 2105 MA1 71 2106 MA1 71 2107 MA1 71 2108 MA1 71 2109 MA1 71 2110 MA1 71 2111 E Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Appendix L Accessories amp Spare Parts Auto Fill Accessory To operate the Auto Fill feature either the low pressure or high pressure valve assem bly is required Each assembly contains the solenoid valve connector cable power cable and tubing and fittings to connect to the syringe Operates at 115 230 VAC 50 60 Hz via a selector switch Catalog No Accessories MA1 55 7760 MA1 70 2022 MA1 72 8340 MA1 55 4144 MA1 55 7002 MA1 55 7004 MA1 61 0270 MA1 72 0199 MA1 72 1405 MA1 70 2263 MA1 70 2264 MA1 70 2265 MA1 70 2266 Description Daisy Chain Cable PHD 22 2000 RS 232 Adapter 9 pin to standard phone jack USB to Serial Connector Foot Switch Auto Fill Valve Box Regular Pressure to 20 psi Auto Fill Valve Box High Pressure 20
9. AND PARTS Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using a part number obtained from our Customer Service department If practical enclose a sample part or sketch We offer a com plete reconditioning service SERIAL NUMBERS All inquiries concerning our product should refer to the serial number of the unit located on the rear panel See Figure 2 CALIBRATIONS All electrical apparatus is calibrated at rated voltage and frequency While the flow and vol ume will stay calibrated the peak pressure may vary AN CAUTION Not for clinical use on human patients Publication 5400 008 REV F s Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual General Safety Summary Please read the following safety precautions to ensure proper use of your syringe pump To avoid potential hazards and product damage use this product only as instructed in this manual To Prevent Hazard or Injury Use Proper Line Cord Use only the specified line cord for this product and make sure line cord is certified for country of use Ground the Product This product is grounded through the grounding conductor of the power cord To avoid electric shock the grounding conductor must be connected to earth ground Before making any connections to the input or output terminals of the product ensure that the product is properly grounded Make Proper Connections
10. Chain Commands Pump Chain Commands Model 44 Protocol Symbol lt lf gt lt float gt lt integer gt lt time gt lt text gt Meaning optional select one either or digits 0 9 or a decimal point digits 0 9 carriage return ASCII 13 line feed ASCII 10 ffffff ddddd d dd dd any string of ASCII characters Command Formats and Meanings Command Format lt cr gt pump address lt cr gt optional pump address command lt cr gt After each command is received and executed the pump acknowledges the command with a prompt Preceding the prompt may be some additional text responses The addi tional text will be one or more lines of ASCII text each preceded by a line feed and terminated by a carriage return lt lf gt lt text gt lt cr gt A prompt is a string of ASCII characters sent by a pump indicating the pumps address and its present state Meaning Stops all pumps All pumps on the pump chain interpret this as a stop command Request for prompt The pump with the indicated address responds with its prompt Send a command to a pump The pump with the indicated address executes the command then responds with its prompt The optional pump address if not specified will default to pump address 0 lt lf gt 1 or 2 digit address prompt character Prompt Characters N ANV gt Publication 5400 008 REV F Meaning Pump stopped Pump infusing Pump refilli
11. Make sure all connections are made properly and securely Any signal wire connec tions to the unit must be no longer than 3 meters Observe all Terminal Ratings Review the operating manual to learn the ratings on all connections Use Proper Fuse Use only specified fuses with product Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qual ified service personnel to perform inspection Place Product in Proper Environment Review the operating manual for guidelines for proper operating environments Observe all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION Protective Ground Refer to Manual Terminal Publication 5400 008 REV F 4 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Specifications Specifications PHD 22 2000 SYRINGE PUMP SERIES Accuracy 0 35 Reproducibility 0 05 Syringes Min Max 0 5 pl 140 ml Flow Rate Minimum 0 0001 yl hr with 0 5 pl Syringe Maximum 220 82 ml min with 140 ml Syringe Calibration Automatic Display 2 line 40 char fluorescent Non Volatile Memory Stores all settings RS 232 Interface Multiplexed dual bidirectional ports RS 232 Connectors RJ11 4 cond phone plug TTL Connectors 9 pin D Sub connector Linear Force Max Standard 23 kg 50 Ibs High Force 30 k
12. SELECT MODE key and press the RUN STOP key The examples on the following RATE pages include Multiple Infusions Ramping Up ml min Infusion Rate Multiple Dispensing Periodic Dispense Loop Combination Infuse and Withdraw Profile Use of Events and Use of Signal 50 Multiple Infusion Example em The following program will instruct the pump to 0 5 10 15 infuse according to the above graph The pro gram instructs the pump to infuse 10 ml at 75 000 mi mn then infuse another 5 ml at 25 ml mn then stop for a total of three sequences Since this graph is Rate vs Volume Strategy 1 will be used when entering the program VOLUME ml Figure 6 Multiple Infusions PROGRAM Key Presses Explanation SET Allows selection of Programming mode PROGRAM Press until PROFILE selected ENTER Enters selection Fe Enter rate of 75 000 ml mn INFUSE RATE Press until units are ml mn ENTER Enters rate 0 ENTER Enter 0 for the time this indicates Strategy 1 10 ENTER 10 ml is the first target volume INFUSE REFILL Toggles direction to infuse ENTER Enters sequence s pumping direction PROG1 SEQ 1 PROFILE 75 000 mi mn 10 000 ml INFUSE PROG1 SEQ 2 PROFILE 25 000 mi mn 5 0000 ml INFUSE PROG1 SEQ 3 STOP SEQUENCE 2 Key Presses Explanation PROGRAM Press until PROFILE selected ENTER Enters selection 25 Enter rate of 25 000 ml mn INFUSE RATE Press until units are ml mn ENTER Enters rate 0 ENTER Ent
13. ULH number Set rate units are microliters per hour MMD number Set diameter units are mm Rate is set to 0 MIT number Set target infusion volume units are ml Numbers between 0 and 1999 will be accepted by the pump Leading zeros and trailing decimal point are optional Any number of digits to the right of the decimal point may be transmitted The number received will be rounded to four significant digits if the leading digit is 1 or three significant digits if the leading digit is 2 to 9 Publication 5400 008 REV F i Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Pump Chain Commands Queries Response CR LF Value CR LF Prompt Queries with Numeric Response DIA Send diameter value units in mm RAT Send rate value in current range units VOL Send current accumulated infused volume units are ml TAR Send target volume units are ml VER Send model and version number Value format nnnn nnn The transmitted value is an 8 character string with leading zeros converted to SP char acters ASCII 32 The fifth character is a decimal point ASCII 46 Query Response CR LF Range CR LF Prompt Queries with String Response RNG Send range message Range is a character string one of ML H ML M UL H or UL M Error Responses CR LF CR LF prompt Unrecognized command CR LF O OR CR LF prompt Out of range Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Pump
14. diameter is accepted and valid diameter becomes new diame ter Diameter will not be accepted if the pump is running and a Not Applicable response will be given If the diameter is invalid an Out Of Range response will be given Query diameter DIA Response is of the following format space space f f f f f f Units are MM TGT lt float gt Request to set or query target volume setting Set target volume TGT volume Volume is of format f f f f f f Units are ML If volume is accepted and valid volume becomes new target vol ume Volume will not be accepted if the pump is running and a Not Applicable response will be given If the volume is invalid an Out Of Range response will be given Query volume TGT Response is of the following format space space f f f f f f Units are ML Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Pump Chain Commands 4 MOD PMP VOL PGM Request to set or query pumping mode Set MOD PMP Puts pump in Pump Mode MOD VOL Puts pump in Volume Mode MOD PGM Puts pump in Program Mode Command will not be accepted if the pump is running and a Not Applicable response will be given Query MOD If mode is PUMP response will be PUMP If mode is VOLUME response will be VOLUME If mode is PROGRAM response will be PRGRAM DIR INF REF REV Request to set or query pumping direction Set DIR I
15. different units EEPROM Data Lost The values stored in the nonvolatile memory have been lost This should never occur under normal operation Replace NOVRAM The nonvolatile memory has failed This should never occur under normal operation Publication 5400 008 REV F 4 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Appendix J Auto Fill Valves There are two Auto Fill Valve models available Catalog No Product MA1 55 7002 Supplied with 1 8 inch ID 1 4 inch OD silastic tubing and connector to syringe MA1 55 7004 High Pressure Supplied with 1 4 inch OD 304 stainless steel tubing with Swagelok fittings Setting Up the Valve 1 Power Connection Connect the line cord to the power supply included Attach the power supply to the input jack on the value box 2 Pump to Valve Electrical Connection The cable fitted with a male 9 pin D sub connector must be connected to the female D sub connector on the rear of the PHD 22 2000 Syringe Pump Power can now be switched on with the illuminated power On Off switch on the front valve housing 3 Pump to Valve Tubing Connection Swagelok fitting should be finger tight plus 1 1 4 turn Tubing is 304 stainless steel 1 4 inch O D wall thickness 0 035 inch Fe Silicon tubing 1 4 inch OD 1 8 inch ID Infuse Refill
16. has been determined to pump 43 155 ml The first sequence refills the syringe with the volume to be infused then the infusion profile is started after which the syringe is refilled and the infusion is repeated until the pump is stopped PROGRAM PRINTOUT PROG1 SEQ 1 PROFILE 75 000 ml mn 43 155 ml REFILL PROG1 SEQ 2 PROFILE 50 000 ml mn 0 00 04 INTERVAL INFUSE PROG1 SEQ 3 DECR 4 0000 DECR 0 00 01 INTERVAL 12 REPEAT INFUSE PROG1 SEQ 4 INCR 5 0000 DECR 8 0000 INCR 0 00 01 INTERVAL 8 REPEAT INFUSE Publication 5400 008 REV F PROG1 SEQ 5 PROFILE 95 000 ml mn 0 00 10 INTERVAL INFUSE PROG1 SEQ 6 PROFILE 30 000 ml mn 0 00 05 INTERVAL INFUSE PROG1 SEQ 7 PROFILE 65 000 ml mn 0 00 05 INTERVAL INFUSE PROG1 SEQ 8 DECR 5 0000 DECR 0 00 01 INTERVAL 11 REPEAT INFUSE PROG1 SEQ 9 RESTART 2 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Programming Tutorial Use of Events 1 Output Pin4 0 A 4 Event Event 5 5 e o 0 Time Figure 11 Use of Events Rate ml mn This is an example of the PHD 22 2000 working interactively with other laboratory equipment The pump will continuously pump af 300 ml hr until an external event a high to low transition at pin 9 possibly produced by another PHD 22 2000 causes the pump to deliver a 15 ml bolus at 75 ml mn After delivering 5 ml of the bolus out put pin
17. if one is not specified in the program regardless of pumping direction When entering the Infuse Rate the INFUSE RATE key is used to scroll through the allow able units of rate The allowable units are ml mn ml hr ul mn ul hr While running in the Pump or Volume modes the Infuse Rate can be changed If the new rate is valid it will take effect when the ENTER key is pressed The minimum and maximum rates permitted vary depending on the diameter of the syringe If an OUT OF RANGE message is displayed when entering a rate try using a different syringe for your application Refill Rate not applicable to the Infusion model The Refill Rate is the rate of pumping while refilling in the Pump or Volume Modes or dur ing Auto Fill If the Refill Rate hasn t been set rate is 0 the Refill Rate will default to the Infuse Rate When entering the Refill Rate the REFILL RATE key is used to scroll through the allowable units of rate The allowable units are ml mn ml hr ul mn ul hr While run ning in the Pump or Volume modes the Refill Rate can be changed If the new rate is valid it will take effect when the ENTER key is pressed The minimum and maximum rates per mitted vary depending on the diameter of the syringe If an OUT OF RANGE message is displayed when entering a rate try using a different syringe for your application Publication 5400 008 REV F 4 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Oper
18. mn for 10 seconds with a profile operation 4 Stop the pump Publication 5400 008 REV F 4 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Programming Tutorial Multiple Dispensing Example 75 SEQUENCE PROGRAM PRINTOUT A t PROG1 SEQ 1 DISPENSE 35 000 ml mn 15 000 ml 3 REPEAT INFUSE PROG1 SEQ 2 DISPENSE 25 65 000 ml mn 25 000 ml 2 REPEAT INFUSE P 7 F PROG1 SEQ 3 DISPENSE l l l 45 000 ml mn 0 EXTERNAL TRIGGERS 17 000 ml TIME Figure 8 Multiple Dispensing gt a 3 AT PROG1 SEQ 4 STOP a RATE ml min Here a series of dispenses are programmed Each dispense is started by a trigger such as pressing the RUN STOP key or pressing an attached foot switch Seven dispenses are programmed three of 15 ml at 35 ml mn two of 25 ml at 65 ml mn and two of 17 ml at 45 ml mn The pump s display will show TRIGGER when it is waiting for a run trigger and lt or gt will not be displayed This is a Strategy 1 dispense A time interval of 0 is specified when entering a Strategy 1 dispense Since the total volume to be dispensed is 129 ml and the syringe volume is 50 ml the Auto Fill feature would be very useful with this program Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Programming Tutorial Periodic Dispense Loop Example RATE ml min 0 TIME
19. of Equipment Model No 73 23 EEC 89 336 EEC EN61010 EN50082 1 EN5008 1 1 Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 U S A Syringe Pump PHD 2000 Series I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place Date C HARVARD APPARATUS United States of America November 11 1996 y Signature Beth Bauman Full Name VP Engineering Operations Position
20. of any type should never be used to clean the pump A mild detergent solu tion may be used to clean the keypad Troubleshooting Infusion Accuracy To ensure infusion accuracy always use new syringe s and measure syringe bore diameter and enter actual dimensions in millimeters mm Additionally make sure that the guide rods and lead screw are properly lubricated RS 232 Difficulties Verify that the baud rates and data framing parameters on all devices are the same With the pump chain a Communication timeout error on a computer is usually caused by the computer errantly handshaking on the RTS CTS and DSR lines Verify pins 4 5 and 6 on the 25 pin connector are jumped on the comput er side of the cable PHD 22 2000 Display Messages Pump Stalled This indicates that the pusher block travel has been impeded This may be caused by the syringe plunger hitting bottom a kink in the tubing syringe plunger binding or any situation requiring more force to the head of the syringe than the pump is capable of delivering To restart the pump remove the cause of stalling and press RUN The pump will continue the interrupted procedure where it stopped Also note a large increase in the pumping rate could also stall the motor Out of Range A value was entered or encountered in a pump program that was beyond the pump s limits Also verify that the Infuse Rate is not 0 Note that rates must be less than 42949 Express larger rates using
21. program SEQ 2 PROFILE 100 00 ml mn 150 00 ml REFILL Query program sequence n s mode SEQ n MOD Response will be according to the following table Response Description Response STP stop RST PRO profile GOT INC increment EVN DEC decrement PMP DIS dispense OUT PAS pause Publication 5400 008 REV F Description restart go to event pump TTL out J Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Pump Chain Commands Query data item of program sequence n Command Description SEQ n RAT Query rate Response f f f f f f units Where units is one of the following ml mn ul mn ml hr ul hr SEQ n GOT Query go to sequence number Response lt n gt SEQ n TGT Query target volume Response lt float gt SEQ n INT Query time interval Response lt time gt SEQ n RPT Query repetition count Response lt float gt SEQ n OUT Query output pin level setting Response lt ON OFF gt SEQ n DIR Query pumping direction Possible responses INFUSE REFILL Set mode of program sequence n SEQ n MOD mode Where mode is as follows Mode Description Mode Description STP stop RST restart PRO profile EVN event INC increment GOT go to DEC decrement OUT set output pin DIS dispense PMP pump PAS pause Set data item of program sequence n Set sequence s rate SEQ lt n gt RAT lt float gt lt units gt Rate is of format f f f f f f Units are one of Description UM ul mn U
22. the pump will not stop when the syringe is full Also if the syringe plunger is manually moved the pump will lose track of the true syringe volume Selecting the Run Mode After entering any necessary operating data into the pump select the pumping mode that will be used when the pump is operated Pressing the SELECT MODE key advances the second line of the display to indicate the next run mode Advance the run mode to the desired mode either Pump Mode Volume Mode or Program Mode until it is displayed Pump Mode The pump will continuously pump infusing or refilling until stopped While running the Infuse and Refill Rates can be changed The new rate for the relevant pumping direction takes effect when the ENTER key is pressed Also the pumping direction can be changed by pressing the INFUSE REFILL key Volume Mode The pump will run infusing or refilling until a specified target volume is pumped or refilled The TARGET VOLUME key is used to enter the Volume Mode target volume Used in conjunction with Auto Fill the target volume can be greater than the volume of the syringe While running the Target Volume Infuse and Refill Rates can be changed The new rate for the relevant pumping direction takes effect when the ENTER key is pressed Target Volume can be used to enter a Target weight in grams if a scale is attached via RS 232 Program Mode In the Program Mode the pump can make complex dispenses including changes in rate and t
23. 0 0 oo 000 o 0o 0 0 0 0 00000 o 00000 o o 0 O O RS 232 HARVARD APPARATUS o ooo vo HOLLISTON MA 01746 OUT N vogs CAUTION Re AMPS NOT FOR CLINICAL USE ol es29 o SERIAL NO CE ON HUMAN PATIENTS a 5006 313 REV 0 _ 508 8335333 WADE IN USA USERI O OUT IN Figure 5 Rear Panel External Connections External devices that can be attached to the PHD 22 2000 are categorized into either RS 232 devices or user I O devices Pump Chains Scales and Printers are RS 232 devices all other devices are user I O devices See the Appendix F for specification details on attaching RS 232 devices and Appendix H for user I O specifications There are two telephone jack type connectors on the back of the unit See Figure 5 These are the RS 232 ports Looking at the back of the pump the connector on the right is IN and the left is OUT Attach the RS 232 connectors in the appropriate port according to the following chart Device Port Pump Chain computer side IN Pump Chain pump side OUT Scale IN Printer OUT Configuring the Pump for RS 232 Devices Press the SET key and then press 1 to select either Model 22 or Model 44 Protocol Refer to Section 12 Pump Chain Commands Press the SET key then use the RS 232 key to scroll through the menu of allowable RS 232 configurations Possible configu rations are Pump Chain Scale Printer Scale amp Printer After entering the RS 232 configuration additional informat
24. 0 00 01 INTERVAL PROG1 SEQ 7 TTL OUT PROG1SEQ 3 TTL OUT ON ON PROG1 SEQ 8 PROFILE PROG1 SEQ 4 PAUSE 75 000 ml mn 0 00 01 INTERVAL 5 0000 ml PROG1 SEQ 5 TTL OUT INFUSE OFF PROG1SEQ 9 GO TO5 PROG1SEQ 6 PROFILE This is an example of the PHD 22 2000 being operated from a remote location When the pump is powered on the position of the pusher block is unknown and must be homed to a known position At the syringe full position a limit switch is placed such that it is tripped by the pusher block when the syringe is full The limit switch is connected to pins 4 and 9 programma ble output and the event input on the user I O connector When the pump receives a start signal it first refills the syringe and stops when the limit switch is sensed The pump then waits for a start trigger and performs a dispense then refills the syringe and waits again for the next start trigger PROGRAM PRINTOUT PROG1 SEQ 1 EVENT 75 000 ml mn GO TO 7 PROG1 SEQ 7 DISPENSE PROG1 SEQ 2 TTL OUT 10 000 ml mn ON 0 0001 ml PROG1 SEQ 3 PAUSE 1 REPEAT 0 00 01 INTERVAL REFILL PROG1 SEQ 4 TTL OUT PROG1 SEQ 8 PUMP OFF 75 000 ml mn PROG1 SEQ 5 PAUSE 10 000 ml 00 00 01 INTERVAL INFUSE PROG1 SEQ 6 PUMP PROG1 SEQ 9 RESTART Publication 5400 008 REV F 2 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Pump Chain Commands The Pump Chain RS 232 interface is used to enhance the control applications of the PHD 22 2000 Thi
25. 0 psi Continuous Flow Tube Segment Remote Extension Cable 1 5 m 5 ft Remote Ext Cable 9 1 m 30 ft SS Syringe 200 ml with 1 16 inch Swagelok SS Syringe 200 ml with 1 8 inch SWAGELOK SS Syringe 200 ml with 1 4 inch SWAGELOK SS Syringe 200 ml with Luer Lock For PHD 22 2000 HVP Remote Syringe Pump For PHD 22 2000 Push Pull Syringe Pump Spare Parts MA1 0606 086 MA1 2400 243 MA1 5012 005 MA1 5091 030 MA1 5100 055 MA1 5153 209 MA1 5400 008 Magnalube Lead Screw Lubricant Syringe Clamp 3 32 Hex Key Syringe Retainer Knob T Knob Withdraw and Anti Siphon Bracket Power Cord PHD 22 2000 Operators Manual Publication 5400 008 REV F s Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Appendix M Kits and Upgrades Catalog No Kits MA1 70 2012 MA1 70 2013 MA1 70 2014 MA1 70 2015 Upgrades MA1 70 2016 MA1 70 2017 MA1 70 2018 Factory Installed Only Description PHD 22 2000 6 10 Multirack Upgrade Kit PHD 22 2000 4 x 140ml Multirack Upgrade Kit PHD 22 2000 Microliter Rack Upgrade Kit PHD 22 2000 Anti Siphon Kit for Infusion Pump PHD 22 2000 Infusion to Infuse Withdraw PHD 22 2000 Infuse Withdraw to Programmable PHD 22 2000 Infusion to Programmable Publication 5400 008 REV F Declaration of Conformity Application of Council Directive s Standard s to which conformity is declared Manufacturer s Name Manufacturer s Address Type
26. 06 220 82 Stainless Steel Syringes Only Harvard s stainless steel syringes can be used with this pump They can withstand high pressure applications and GEIGRIIS CURT eel Stel provide years of service Flow Rates for PHD 22 2000 HVP High Volume and Pressure Syringe Size Minimum Maximum 20 ml 1 5 yl hr 20 ml min 50 ml 3 4 ul hr 46 ml min 100 ml 5 0 uV hr 68 ml min 200 ml 8 2 ul hr 112 ml min Publication 5400 008 REV F z Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Appendix D Custom Applications The Harvard PHD 22 2000 Syringe Pumps lend themselves to a multitude of OEM industrial applications For all types of custom pumping or pilot plant applications the pump s programming capabilities can be enhanced Please contact the Harvard Development Group if we can be of help Appendix E PHD 22 2000 to PC Connections m ee CE RJ11 Cable Straight Thru DB9 Female to Computer View at Pump Pinning 4 6 PIN RJ11 12 such as Max Distance L COM P N RBAO98F 15M Front View Computer Connection Terminal Port Chart Settings RJ12 6x6 RJ11 RJ12 DB9 STD DBS Baud Rate 9600 2 RD or match pump baud rate 3 TD Data Bits 8 5 Sig ground Stop Bits 2 RJ11 6x4 View of Cable Adapter Figure 13 Pump to PC Connection Publication 5400 008 REV F 4 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Appendix F RS 232 Specificat
27. 20 200 ml MA1 72 2472 Replacement Viton O Ring 20 ml pkg of 10 MA1 72 2473 Replacement Viton O Ring 50 ml pkg of 10 MA1 72 2474 Replacement Viton O Ring 100 ml pkg of 10 MA1 72 2475 Replacement Viton O Ring 200 mi pkg of 10 MA1 72 2616 Replacement Viton Tip Seal O Ring sizes 20 200 ml pkg of 20 MA1 72 2617 Stainless Steel Plunger Button to Adapt Syringe for Use with PHD 22 2000 HVP with Triple S Clamp Required for 50 ml and 100 ml syringes only Fitting for Tube Diameter Syringe Size 1 16 inch Harvard High Pressure Stainless Steel Syringes 1 8 inch Harvard High Pressure Stainless Steel Syringes Harvard now offers a completely new line of Stainless Steel Syringes intended for high pressure applications with good resistance to most aggressive liquids Wetted parts are 316 stainless steel or Viton Syringes are available in 20 50 100 and 200 ml sizes with removable replace able tips Syringe to tube fittings are avail able in 1 16 1 8 and 1 4 inch sizes A luer lock end fitting is also available All tips are interchangeable with all syringes 20 to 200 ml in the series Both syringe barrel end plungers are 316 stainless steel A Viton O ring seal between top and end of the barrel insures against leakage Syringes are guaranteed to be leak free for pressures up to 700 psi All syringes are supplied with inside diameter dimensions for use with Harvard microprocessor controlled pump
28. 22 and Model 44 pumps to use existing pro grams to control the PHD 22 2000 pumps The two sets of commands are contained on the following pages Publication 5400 008 REV F 4 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Pump Chain Commands Each command sent to the pump chain is a string of ASCII characters with leading zero s on numbers and all spaces optional Numbers are a maximum of five digits The following symbols are used in describing the commands Pump Chain Commands Model 22 Protocol Model 44 Protocol on page 39 After each transmission to the Pump terminating with a CR character ASCII 13 the pump enters remote mode and responds with the three character sequence CR LF prompt The prompt character indicates the status of the pump as follows When stopped ASCII 58 decimal gt When running forward ASCII 62 decimal lt When running reverse ASCII 60 decimal When stalled ASCII 42 decimal Note With a pump chain if no address precedes the command trans mitted the pump at address 0 will respond to the command Commands Response CR LF prompt RUN Infuse forward direction REV Start reverse direction Not accessible on Infusion model STP Stop CLV Clear volume accumulator to zero CLT Clear target volume to zero MLM number Set rate units are milliliters per minute ULM number Set rate units are microliters per minute MLH number Set rate units are milliliters per hour
29. 2b Remote Unit Connection 06 15 Figure 3 Fuse Replacement 16 Figure 4 Syringe Loading cccceeeeeeeeeeees 18 Figure 5 Rear Panel External Connections 27 Figure 6 Multiple Infusions 0 ee 29 Figure 7 Ramping Up Infusion Rate 30 Figure 8 Multiple Dispensing ee 31 Figure 9 Periodic Dispense Loop 000 32 Figure 10 Comb Infusion amp Withdraw Profiles 33 Figure 11 Use Of EVES iis ciscrnscavomsccceninecscrzarentees 34 Figure 12 Use of TTL Signal 0 eeeeeee 35 Figure 13 PHD 22 2000 to PC Connection 52 Figure 14 RS 232 Specifications 0 eee 53 Figure 15 User I O Connector Specifications 54 Figure 16 Auto Fill Valves 0 000 000 eeeeeeeeeeee 56 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Warranty and Repair Information CAUTION REFER TO SECTION 7 INITIAL SETUP BEFORE PLUGGING IN PHD 22 2000 PUMP MANUAL DESCRIPTION This manual is designed to provide all operational and program information required to operate and maintain the PHD 22 2000 series pumps The functions and features are described in the Technical Specifications section WARRANTY Harvard Apparatus warranties this instrument for a period of two years from date of pur chase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or materials This warra
30. 4 is set to a logic high for the duration of tbe bolus after which it is dropped This output pin can be attached to the timer input of another pump such as a Harvard Pump 22 or 44 to create a precise mixture during the bolus After the bolus is com pleted the Pump 22 would be stopped and the PHD 22 2000 would return to deliv ering its background rate of 300 ml hr waiting for another external event PROGRAM PRINTOUT PROG1 SEQ 1 TTL OUT 5 0000 ml OFF INFUSE PROG1 SEQ 2 EVENT PROG1 SEQ 5 TTL OUT GO TO 4 ON PROG1 SEQ3 PUMP PROG1 SEQ 6 PROFILE 300 00 ml hr 75 000 ml mn INFUSE 10 0000 ml PROG1 SEQ 4 PROFILE INFUSE 75 000 ml mn PROG1 SEQ 7 RESTART Publication 5400 008 REV F 2 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Programming Tutorial Time Figure 12 Use of TTL Signal Before the pumping flow begins the pump sends a TTL signal to another piece of equip ment for 5 seconds but first verifies that the TTL line is low by turning it off for 1 second then turns it on The pumping process consists of an alternation of pumping 3 ml s at 53 ml mn and 5 ml s at 75 ml mn It uses the TTL output to signal to another device whose sequence it is executing by lowering the TTL line before sequence 5 and raising it before sequence 8 PROGRAM PRINTOUT PROG1 SEQ 1 TTL OUT 53 000 ml mn OFF 3 0000 ml Prog1 SEQ 2 PAUSE INFUSE
31. H ul hr MM ml mn MH ml hr Publication 5400 008 REV F 4 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Pump Chain Commands Set sequence s go to sequence number SEC lt n gt GOT lt n gt Set sequence s target volume SEQ lt n gt TGT lt float gt Set sequence s time or target SEQ lt n gt INT lt time gt Set sequence s repetition number SEQ lt n gt RPT lt integer gt Set sequence s pumping direction SEQ lt n gt DIR lt INF REF gt Set sequence s output pin level SEQ lt n gt OUT lt ON OFF gt VER Request for version of pumps embedded software Pump Chain Error Messages Error messages are in the format lt lf gt space space lt message gt lt cr gt Where lt message gt is one of the following Syntax error in a received command NA Command not applicable at this time OOR Control data is out of the operating range of the pump Note If assistance is needed in programming pump control via RS 232 call Harvard Apparatus Customer Service Support at 1 800 272 2775 Outside U S call 1 508 893 8999 Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Appendix A Syringe Diameters in mm Stainless Steel Terumo SGE Scientific Size Diameter Size Diameter Glass 7 Engineering 2 5 CC 4 851 mm 3 cc 8 95 mm 20 19 130 5 13 00 Size Diameter 50 28 600 10 15 80 25 ul 0 73 mm 100 34 900 20 20 15 50 1 03 200 44 755 30 23
32. NF sets pumping direction to infusion DIR REF sets pumping direction to refill DIR REV reverses current pumping direction Command will not be accepted if the pump is running in volume or pro gram modes and a Not Applicable response will be given Query DIR If pump direction is infusion response will be INFUSE If pump direction is refill response will be REFILL AF ON OFF Request to set or query auto fill setting Set AF ON turns Auto Fill feature on Note The syringe volume is also needed for auto fill to operate See SYR command AF OFF turns Auto Fill function off Command will not be accepted if the pump is running and a Not Applicable response will be given Query AF If Auto Fill function is ON response will be ON If Auto Fill function if OFE response will be OFF Publication 5400 008 REV F E Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Pump Chain Commands SYR lt float gt Request to set or query syringe volume setting for auto fill Used in conjunction with Auto Fill feature See AF command Set syringe volume SYR volume Volume is of format f f f f f f Units are ML If volume is accepted and valid volume will become the new syringe Auto Fill volume Volume will not be accepted if the pump is running and a Not Applicable response will be given If the volume is invalid an Out Of Range response will be given Query syringe volume SYR
33. PHD 22 2000 Syringe Pump Series User s Manual PHD 22 2000 Series MA1 70 20xx PHD 22 2000 High Force Series MAI 71 20xx PHD 22 2000 Remote Series MA1 70 21xx PHD 22 2000 High Force Remote Series MAI 71 21xx PHD 22 2000 Hpsi MA1 70 2023 HARVARD APPARATUS Publication 5400 008 REV F WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Categ
34. Program Runtime Error Messages If while running a program an operation is requested that cannot be performed the pump will stop and an error message will be displayed Error messages will be dis played with the following format Program N SEQ n message Where N is the Program number and n is the sequence number when an error was detected and message is the indicated error as follows INFINITE LOOP A GOTO sequence cannot specify the current sequence INVALID GO TO The target of the GO TO specified an invalid sequence number RATE UNDERFLOW A decrement sequence decremented a rate to less than or equal to 0 RATE OVERFLOW An increment sequence caused an arithmetic overflow OUT OF RANGE Specified or calculated rate is beyond the pumps capabilities with the specified syringe VOL TGT ERROR A sequence with a volume target cannot follow a sequence with a time target unless the volume delivered is zero or the pump is stopped at the start of the sequence enter ing it select Program Mode using the SELECT MODE key and press the RUN STOP key Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual External Control Interfaces RS 232 Devices Remote Unit Connector MA1 70 21xx MA1 71 21xx and MA1 70 2023 only EEEN o o 000 o 0o00 0 0 o 0o 0 0 0 o o 0 0
35. Pump Mode change alarm mode 4 XXXXX ml INFUSE Press ENTER to accept and Pump Mode return to main display Step Key Setting Power Up Mode Description 1 SET SET WHAT Press and release SET key Pump Mode 2 3 POWER UP STANDBY Press and release 3 key Pump Mode Current power up mode displayed 3 3 POWER UP RUNNING Press and release 3 key to Pump Mode change POWER UP mode 4 XXXXX ml INFUSE Press ENTER to accept and Pump Mode return to main display Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Initial Setup ATE O 000o O O O O O 0 O O O O 0 2 O O O O O O O O O O 0 O O O O O O O O O O O O O O O O o RS 232 HARVARD APPARATUS 0000 10 HOLLISTON MA 01746 OUT CATH VOLTS CAUTION FREQ AMPS NOT FOR CLINICAL USE ole222o SERIAL NO ON HUMAN PATIENTS gene tT F 5006 313 REV 508 897 H095 BADE NUSA Power Cord Socket Main Power Switch Serial Number Label Figure 2a Rear Panel Power Entry and Serial Number Label 1 Read the entire manual to become familiar with all features and functions of the PHD 22 2000 2 The operating voltage range for the PHD 22 2000 is 100 240 VAC 50 60 Hz Use only the specified line cord for this product and make sure line cord is certified for country of use 3 Take this opportunity to fill out and m
36. Response is of the following format space space f f f f f f Request to read the TTL logic level of the specified pin on the external 9 pin D SUB connector Valid pin numbers for input are 6 7 8 and 9 If the pin specified is valid and if the pin level is high response will be ON If the pin level is low response will be OFF If the pin specified is invalid An Out Of Range response will be given OUT d lt ON OFF gt Request to set the TTL logic level at the specified pin on the external 9 pin D SUB connector Valid pin number for output is 4 If the pin specified is invalid An Out Of Range response will be given Example Set pin 4 high OUT 4 ON Set pin 4 low OUT 4 OFF Publication 5400 008 REV F 4 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Pump Chain Commands SEQ lt integer gt lt entry gt Request to set or query programming sequences lt integer gt is sequence number Default is Sequence 1 Command only applicable while pump is stopped Valid sequence numbers n are 1 to 10 n defaults to 1 wherever it is optional See program examples Query entire program SEQ Example response SEQ 1 DISPENSE 75 000 mi mn 43 155 ml 0 00 01 INTERVAL 3 REPEAT INFUSE SEQ 2 PROFILE 100 00 mli mn 150 00 ml REFILL SEQ 3 RESTART Query program sequence n SEQ n Example response to the command SEQ 2 with the previous example s
37. agaseds 9 Program St ra gE issawsseencaansesicarnrescanssousrwarusavesansans 9 User Interface Description of Keys ceicsnsitivatasncceiiesnuanimcaianad 11 13 Special Features Table cccccsssseeeseneeees 13 Entering Data Seateratoeciantverimastaernaavensnrtnadavnnten 14 Initial Setup Fuse Replacement ccccccsssssesecseceeeeeesseees 16 Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Table of Contents SUBJECT PAGE NO Operation Syringe Loading eeesooneeeenneeneeenneene neen 17 19 Running the PUMP wicscaevearcevseeammsnretanearenuevenmanans 20 DIGBY sssrin aE ERRA 20 Infuse Rate sssrinin 20 Refill RANG icsdsicencshandinedvcadcedsnesnardivbevnenciconseuananns 20 Target VOWS xiciinetisncusanainatonvataaiaadanneauseimnaneinan 21 A to PN ssisisciseconan gictesewasutnndscacsGunnsatenaadexsicseeiudeeses 21 Selecting the Run Mode a ecescscecese ees dicasesceemaders 21 Program Mode Program Description in cexsentrnneansesacnannsindnaninrecerntaan 22 Entering a PF OGRA ssiarretersearsisitensiatiantiakwancatiemss ities 22 Sequence Operation ccecseeeeceeeeeees 23 26 Program PriIMMOUT sisciintiincsaiitinsaesidatdannavdidanaindinan 26 Program Run Time Error Messages 26 External Control and Interfaces RS 232 Devices decsuscesssssaesdeascansxaascassauseednanien 27 Configuring Pump for RS 232 Devices 27 28 User WO Devi
38. ail the Warranty Card The Catalog and Serial numbers are located on the Serial Number label on the rear panel Refer to Figure 2a 4 Turn on main power switch see Figure 2a located directly above the power cord receptacle on the rear panel The two line display will illuminate indicating that the power connections are correct The flashing display indicates that power has just been applied Pressing any key will reset the blinking display to constant illumination 5 See Section 8 for operating Instructions on next page 6 Fuse Replacement Turn off power remove power cord from power module Use straight blade screw driver pry open access door Remove fuse holder remove fuse s from holder Replace fuse s and fuse holder For continued fire protec tion replace fuse only with 250 V fuse of the specified type and rating 3 AG 0 50 AMP SLO BLO See Figure 3 7 Remote Unit Connection Catalog no s MA1 70 21xx MA1 71 21xx and MA1 70 2023 only Connect the control unit to the remote pump unit with the cable provided Remote Extension Cable Remote Pump Unit Figure 2b Remote Unit Connection Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Operation Screw Driver Figure 3 Fuse Replacement Because of the wide range of functions that the PHD 22 2000 is capable of perform ing certain information about your application must be entere
39. al target An incremental target is added to the delivered volume at the start of the sequence Time Interval Enter the time duration of the sequence in the form hours minutes seconds If sequence Strategy 1 Target Volume is used enter 0 00 00 for the time target The max imum time interval is 9 99 99 Number of Repetitions Enter the number of times the sequence is to be repeated The repetition number can be from 1 to 99 999 Pumping Direction Each sequence that specifies a pumping operation also specifies a pumping direction Use the INFUSE REFILL key to change the pumping direction lt or gt Pin Level Select either HI or LOW for the logic level of the programmable output pin 4 Use the PROGRAM key to change the setting Go to sequence number Enter the destination sequence to continue operation of the program Valid sequence numbers are 1 to 9 Publication 5400 008 REV F 2 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Program Mode Profile Runs at specified flow rate until target volume is pumped or a time interval has elapsed Travel direction is as specified Data Specified Strategy 1 Flow rate Volume Time Interval 00 00 00 Target volume Pumping direction Strategy 2 Flow rate Time Time interval Pumping direction Incr Increments current rate by specified value and pumps until the target volume is pumped or a time interval has elapsed Units of rate will be tha
40. arget volume These complex dispenses are easily programmed from the keypad and are detailed in the Program Mode and the Programming Tutorial sections Publication 5400 008 REV F 3 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Program Mode Program Description A program is made up of a set of sequences Each sequence being a set of operating instructions for the pump to follow When the pump is started in the PROGRAM run mode the pump will start at sequence 1 and execute the operating instructions in that sequence When the pump has completed the instructions for a sequence it will go to the next or specified sequence and execute the instructions in that sequence The pump continues this process until it either has reached a STOP operation the pump is manually or remotely stopped or the last sequence has been completed 4 programs with up to 9 sequences each may be entered A sequence consists of a sequence number indicating the order of the sequence a mode indicating what operation the sequence will be performing and the actual data for the operation such as rates and volumes The necessary data specified for each sequence will depend on the strategy used One of two strategies may be chosen for a sequence s target Strategy 1 Target Volume pumps until a target volume is reached while Strategy 2 Time Interval pumps until a target time interval has lapsed When Strategy 1 is used enter a time interva
41. ation Target Volume The Target Volume is the volume that you desire to deliver at the set Infuse or Refill Rate The pump must be set for this Volume mode for this feature to work When entering the Target Volume enter the volume desired in mls milliliters and press the ENTER key Auto Fill Programmable model only Use AUTO FILL key to toggle between Auto Fill ON and OFF If Auto Fill is set to ON the pump will next request the volume of the syringe in milliliters The volume of the syringe is used as the refill volume of the syringe This volume can be set to a volume smaller than the syringe if desired When set to ON the syringe is assumed to be empty Auto Fill continuously monitors the volume of the syringe according to the volume pumped When the pump determines that the syringe is empty the operation in progress is suspended and Auto Fill is activated The pumping direction is then reversed and the pump runs at the refill rate During the Auto Fill operation the display will indicate the volume of liquid in the syringe When the volume in the syringe reaches the set refill volume Auto Fill will stop and the previous operation of the pump will resume Auto Fill continues to monitor the volume of the syringe TTL direction output is toggled ON during refill Refill Rate defaults to Infuse Rate if not set Note Auto Fill will only activate while infusing i e if the pump direction is set to Refill
42. ces ix icetuiccsiiornanveuicniccdaviearcalseunirieans 28 Programming Tutorial Multiple Infusion Example c ceeeeee 29 Ramping Up Infusion Rate Example 30 Multiple Dispensing Example 008 31 Periodic Dispense Loop Example 32 Combination of Infusion and Withdraw Profiles EXAMS siciontss cansininaaitunitricuiermarniautennsaaiaua 33 Use OF Events ecesdesecunicececnesvsteinaxemmataamcteiess 34 35 Pump Chain Commands Model 22 Protocol sicsiigcita ctncctenscwnsinantatdnauaninns 37 38 Model 44 Protocol ais ciasticcutencennaseevonsttencemnuenemdives 39 Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Table of Contents SUBJECT PAGE NO Appendices A Table of Syringe Diameters 48 B Stainless Steel Syringe 00 49 50 C Nominal Min Max Flow Rates 51 D Custom Applications ccccceeceees 52 E PHD 22 2000 to PC Connection 52 F RS 232 Specifications csc0 scccsccccs 53 G Symphony ssscctnsasiccssachanesscctantarestadtevenicets 53 H User I O Connector Specifications 54 l Maintenance and Troubleshooting 55 J Auto Fill Valves cceeecceceeeeesseseereeees 56 K L M Accessories and Spare Parts 57 59 Figure 1 Keypads iccctasccrascecincsscseressonorcanmenasenanest 11 Figure 2a Rear Panel and Serial Number Label 15 Figure
43. d into the pump At min imum the pump needs to know the diameter of your syringe the infusion rate and direction of travel This is the only information needed to run the pump in the Pump Mode If not speci fied the refill rate will default to the infuse rate The pump will need additional infor mation to utilize its more advanced features See Section 6 User Interface for general information on data entry CAUTION Use in manner not specified by the manufacturer may impair the protection provided by the equipment Publication 5400 008 REV F 4 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Operation SYRINGE LOADING Standard with a 2 Syringe Holder The Pusher Block 2 and Syringe Holder Block are fitted with movable retaining brack ets 4 and 5 Available as an option for the PHD 22 2000 Infusion model as an Anti siphon kit Catalog MA1 70 2015 which hold the syringe barrel and plunger firmly when refill ing When loading the syringe into the pump it is necessary to adjust these retaining brack ets and the Adjustable Stops 8 amp 9 The Pusher Block 2 is fitted with Release Tabs 1 to release the drive nut from the leadscrew so that the Pusher Block 2 can be moved freely on the leadscrew to facilitate loading the syringe Refer to Figure 4 1 Squeeze the Release Tabs 1 towards each other and move the Pusher Block 2 to the left allowing enough space for the syringe being used to fit betwe
44. e Doing so may crack or break a glass syringe Ensure that both retaining brackets 4 and 5 are tight against the syringe barrel flange and plunger flange then tighten the screws on the retaining brackets on the Pusher Block 2 and the Syringe Holder Block 3 This step is not required for the PHD 22 2000 Infusion model which is not equipped with retaining brackets or Adjustable Stops Squeeze the Release Tabs 1 and move the Pusher Block 2 to the right until the syringe plunger is located as far into the syringe as it will travel Then squeeze the Release Tabs 1 and move the Pusher Block 2 to the left just enough so that the end of the syringe plunger is not touching the inside end of the syringe barrel and release the Release Tabs 1 This step is not required for the PHD 22 2000 Infusion model which is not equipped with retaining brackets or Adjustable Stops Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Operation eP ee SH RELEASEO ENGAGED 1 Release Tabs 6 Syringe Clamps disengage 2 Pusher Blocks 7 Allen Wrench 3 Syringe Holder 8 Adjustable Stops aS Block Infuse i 4 Retaining Bracket 9 Adjustable Stops Pusher Block Refill 5 Retaining Bracket Syringe Block
45. e f f f f f f units Where units is one of the following ml mn ul mn ml hr ul hr Publication 5400 008 REV F 4 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Pump Chain Commands RFR lt float gt lt units gt Request to set or query refill rate setting Set refill rate RFR rate Set refill rate and units RFR rate units Rate is of format f f f f f f Units are one of Definition UM ul mn UH ul hr MM ml mn MH ml hr If rate is accepted and valid rate will become the new refill rate If the rate is invalid an Out Of Range response will be given Command will not be accepted if the pump is running in the Program Mode and a Not Applicable response will be given Query refill rate RFR Response is of the following format space space f f f f f f units Where units is one of the following ml mn ul mn ml hr ul hr PGR Request for the rate of pumping set during the running of a program Response is of the following format space space f f f f f f units Where units is one of the following ml mn ul mn ml hr ul hr Publication 5400 008 REV F 3 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Pump Chain Commands DIA lt float gt Request to set or query syringe diameter setting Set diameter DIA diameter Diameter is of format f f f f f f Units are MM INFUSE and REFILL rates will be set to zero and AUTO FILL will be set to off If
46. een outlined SET key The dis play should then read SET WHAT Press the key in the SET key group whose data is to be modified The display will display the current setting Data is entered into the pump by either entering a numerical value or by scrolling through a menu of choices Always press the green outlined ENTER key to terminate and store each data request by the pump If you are to enter a numerical value the far left of the display will show ENTER fol lowed by the units of the number to be entered Using the numerical keys on the right side of the keypad see Figure 1 enter the new data value Up to five digit numbers are accepted including up to four decimal places Entering more than five digits will clear the previous five digits entered in the display Press the green outlined ENTER key when the desired data value is displayed If the far left of the display does not show ENTER then a menu of choices is being displayed Pressing the relevant key according to the choices being displayed selects successive menu entries When the desired selection is displayed press the green out lined ENTER key If the data value entered is outside the pump s operating parameters the display will read OUT OF RANGE Pressing any key will restore the display with the original data value Enter another data value within the pump s parameters or just press the green outlined ENTER key to reuse the original data value
47. en the Pusher Block 2 and the Syringe Holder Block 3 Loosen the four screws to free the retaining brackets 4 and 5 on the Pusher Block 2 and Syringe Holder Block 3 This step is not required for the PHD 22 2000 Infusion model which is not equipped with retaining brackets 4 amp 5 or Adjustable Stops 9 Locate Syringe Clamp 6 and determine if clamp is in the correct position to firm ly hold the desired syringe Rule of thumb is that if the syringe is 20ml or larger the tips of the clamp should be pointing down If it is required to change the orienta tion of the clamp unscrew the plastic knob located in the center of the clamp by turning it counter clockwise to the left Remove the knob lift the clamp off the shaft flip the clamp over and place back on the shaft then tighten the knob by turn ing it clockwise to the right Lift up on the spring loaded Syringe Clamp 6 and rotate it 90 to allow clearance for the syringe Place the barrel of the syringe on to the Syringe Holder Block 3 and rotate the clamp over the syringe Ensure that the syringe barrel flange and plunger flange are located within the retaining brackets on both the Pusher Block 2 and the Syringe Holder Block 3 This step is not required for the PHD 22 2000 Infusion model which is not equipped with retaining brackets or Adjustable Stops 9 Lower the Syringe Clamp 6 down onto the syringe CAUTION Do not let the Syringe Clamp snap down on the syring
48. enter the value in millimeters Otherwise access the built in Syringe Table and select the syringe being used After a new diameter is entered directly or via the built in Syringe Table the Infuse Rate and Refill Rate are set to 0 and the Auto Fill feature is turned off This is done for reasons of safety The max imum diameter that can be entered is 99 mm To access the built in Syringe Table after pressing the SET key then the DIAMETER key hold down the DIAMETER key for about one second Once the Syringe Table has been accessed each press of the key will scroll through the manufacturers Find the manufacturer and material if applicable of your syringe Press the ENTER key to enter your selection Now using the DIAMETER key again scroll by pressing the DIAMETER key until the size of your syringe is displayed in cc or uL as indicated on the display Pressing the ENTER key will select the size of the syringe and look up and store the diameter The diameter will be dis played until the ENTER key is released Thereafter pressing the DIAMETER key will display the selected diameter In addition the syringe size selected becomes the default Refill Volume when the Auto Fill feature is turned on See Appendix A for a listing of the built in Syringe Table and their respective diameters Infuse Rate The Infuse Rate is the rate of pumping while infusing in the Pump or Volume modes Also the Infuse Rate is used as a starting rate for the program model
49. er 0 for the time this indicates Strategy 1 5 ENTER 5 ml is the second target volume INFUSE REFILL Toggles direction to infuse ENTER Enter sequence s pumping direction SEQUENCE 3 Key Presses Explanation PROGRAM Press until STOP selected ENTER Enters selection and ends program entry Publication 5400 008 REV F Programming Tutorial Ramping Up Infusion Rate Example PROGRAM PRINTOUT 20 PROG1 SEQ 1 PROFILE 10 000 ml mn 0 00 01 INTERVAL INFUSE PROG1 SEQ 2 INCR 0 1695 INCR 0 00 01 INTERVAL a RATE ml min a INFUSE 59 REPEAT PROG1 SEQ 3 PROFILE o 20 000 mli mn 0 00 10 INTERVAL INFUSE TIME seconds PROG1 SEQ 4 STOP Figure 7 Ramping Up Infusion Rate In this example the pump will ramp up from 10 ml mm to 20 ml mn over 60 seconds then continue to run at 20 ml min for another 10 seconds This is a Strategy 2 Program requiring four sequences 1 Specify the initial rate as a profile of 10 ml mn for one second 2 Specify the ramp up to 20 ml mn Since the minimum resolution of an increment is one second it will take 59 steps to reach the target rate Sequence 2 starts at time 1 second and ends at time 60 seconds giving it a duration of 59 seconds At one second a step 59 seconds divided by one second per step equals 59 steps The increase per step will by 20 ml mn minus 10 ml mn divided by 59 steps or 0 1695 rounded to four decimal places 3 Continue running at 20 ml
50. erface HARVARD PHD 22 2000 APPARATUS Infusion et et elect E de Figure 1a Infusion Model Keypad HARVARD PHD 2000 APPARATUS Infuse Withdraw Clore ES EA EA Figure 1b Infuse Withdraw Model Keypad HARVARD PHD 22 2000 APPARATUS Programmable oe A ED Gs Figure 1c Programmable Model Keypad Publication 5400 008 REV F 3 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual User Interface Set Keys SET Allows modification of a data item in this group of keys To modify a data item press the relevant key after pressing the SET key Pressing Set with an appropriate data entry key will also allow the selection of special features Refer to Entering Data on page 13 INFUSE RATE Displays sets current infuse rate Scrolls through rate units with each press of the key when setting Displays current programmed rate while running in program mode TARGET VOLUME Displays sets current volume mode target volume PROGRAM Displays current program number program sequence and sequence number Held down with a printer attached and the pump stopped prints a program listing DIAMETER Displays sets current syringe diameter When held down during setting accesses built in syringe table REFILL RATE Displays sets current refill rate Scrolls through rate units with each pr
51. ess of the key when setting RS 232 Displays sets current RS 232 device s attached AUTO FILL Turns Auto Fill feature setting on off Also displays sets syringe refill volume Toggle Keys C in this group when permitted successive states of the keys function are selected when the key is pressed RUN STOP Starts stops interrupts running of pump INFUSE REFILL Changes direction of pusher block travel during operation in Pump and Volume modes Pump must be stopped or in Pump Mode to reverse direction of pump SELECT MODE Toggles in an incremental loop through PUMP VOLUME or PROGRAM run modes with each press of the key The current mode is displayed on the 2nd line of the display Data Entry Keys O 1 2 3 4 5 6 7 8 9 0 Used to enter numeric data values or access special features ENTER Saves and stores displayed data value in memory when setting a data item Publication 5400 008 REV F 3 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual User Interface Set Keys Keys with rectangular outlines in this group are used to modify or review settings of the pumps control data To review the current setting of a control data item simply hold down the relevant key and the data setting will appear in the top line of the dis play In the case of the PROGRAM key if it is held down the data will be sent to the printer if one is attached To modify a data setting first press then release the gr
52. g 66 Ibs Drive Motor 1 8 Stepper Motor Motor Drive Control Microprocessor from 1 2 to 1 32 microstepping Motor Step per one From 800 to 12 800 Rev of Lead Screw Step Rate Minimum 27 3 sec step Maximum 416 7 usec step Pusher Travel Rate Minimum 0 18 m min Maximum 190 676 mm min Power 100 240 VAC 50 60 Hz 50 W 0 5 A fuse Dimensions 16 3 x 22 8 x 27 9cm 6 4 x 9 x 11in Syringe Holder N A Remote Cable Length N A Weight 4 5 kg 10 Ib Publication 5400 008 REV F PHD 22 2000 VHP REMOTE SYRINGE PUMP SERIES 0 5 0 05 Holds 20 to 200 ml stainless steel syringes 1 5 pl hr 112 ml min Automatic 2 line 40 char fluorescent Stores all settings Multiplexed dual bidirectional ports RJ11 4 cond phone plug 9 pin D Sub connector 196 kg 433 Ibs N A 1 8 Stepper Motor geared Microprocessor from 1 2 to 1 32 microstepping From 8 000 to 128 000 27 3 sec step 416 7 usec step 0 09 m min 71 210 mm min 100 240 VAC 50 60 Hz 50 W 0 5 A fuse 9 9 x 27 9 x 22 9 cm 3 9 x 11 x 9 in 22 9 x 43 2 x 30 5 cm 9x 17 x 12 in 152 cm 60 in approx Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Theory of Operation All models in the PHD 22 2000 series employ a microcontroller which controls a small step angle stepping motor that drives a lead screw and Pusher Block Micro stepping techniques are employed to further reduce the step angle eliminating flow pulsatio
53. ge Pump Series User s Manual Features Pressure and Speed PHD 22 2000 can deliver up to 220 82 ml minute with a single 140 ml syringe Maximum pressure is dependent on syringe size Built In Syringe Table and Custom Syringes The user may select the syringe to be used from the pump s built in syringe table Syringes are arranged according to manufacturer and material and then according to size The pump will look up and use the diameter for the syringe you select See Appendix A for a listing of the standard syringes If a syringe is to be used which is not listed in the syringe table enter the inside diameter of the syringe in millimeters Infusion and Refill Rates Specify independent rates for infusing and refilling This allows a slow infusion rate then a fast refill If Refill Rate is not set the software defaults to the set Infuse Rate value Target Volume Specify the volume that is to be infused or refilled The pump will run at the rate spec ified until this volume has been delivered when in the Volume mode Auto Fill Auto Fill automatically activates an externally attached solenoid refer to Appendix L for part number and refills the syringe when it is empty This permits infusions to be virtually independent of syringe capacity Modes of Operation Pump Runs continuously in the infuse or refill directions until stopped Volume Runs until a specified volume has been pumped or refilled Program Pump operate
54. ion may be requested Pump Chain Enter the 2 digit address assigned to the pump Note Each pump in the chain needs a unique address After entering the address up to 99 the baud rate will be requested Use the RS 232 key to toggle between the supported baud rates 1200 2400 9600 and 19200 Note Each pump in the chain must have same baud rate See the Section 12 Pump Chain Commands for pump chain control information Publication 5400 008 REV F i Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual External Control Interfaces Scale Use the RS 232 key to toggle between the supported manufacturers Mettler Sartorius and Ohaus When a scale is attached the weight will be read from the scale and used as the delivered volume whenever the pumping direction of the pump is set to infuse When refilling the syringe diameter is used for volume calculations When the scale weight is displayed the units will be grams Printer No additional information requested when entering With a printer capable of serial port communication attached the pump will print the delivered volume whenever the pump stops or the direction of pumping changes except before and after Auto Fill of the syringe If the pump stops due to the pump stalling an asterisk will be appended to the volume printed In addition the entered pump program can be listed on the printer by pressing the PROGRAM key for about one second with the pump stopped U
55. ions Pump Chain Pump Port 1 Computer control side Pump Port 2 Connection for remainder of pump chain Baud Rate 1200 2400 9600 or 19 200 Word Size 8 Parity none Stop Bits 2 Scale Connect scale to Pump Port 1 Supported scales Mettler Baud Rate 2400 Word Size 7 Parity even Stop Bits 1 Pause 0 Sartorius Baud Rate 2400 Word Size 7 Parity even Stop Bits 1 Code Settings 311 Weigh only models with PLUS performance package 211 External print command with out stability 225 2400 baud 234 Even Parity 432 Beeper off Transmit Receive GND GND GND GND Transmit Receive Port 2 out in a Port 1 Figure 14 RS 232 Specifications Ohaus Baud Rate 2400 Word Size 8 Parity even Stop Bits 1 Auto print feature off Stable data only off Serial data frame selection Fr 7 Printer Serial printer with print buffer Baud Rate 2400 Word Size 8 Parity none Stop Bits 2 Appendix G Symphony Symphony the Windows Pump Manager software allows you to control your PHD 22 2000 pump from your remote computer With Symphony you can orchestrate all pump functions from a virtual control panel via the serial communications link built into every PHD 22 2000 pump Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Appendix H User I O Connector Specs User I O input
56. l of 0 00 00 then you will be prompted for the target volume See the Programming Tutorial for example programs Entering a Program It is advisable to plan out your program prior to entering the program into the pump Press the SET key then the PROGRAM key to begin entering a program The following is a list of possible data that can be requested when entering a program and instructions on entering the data Publication 5400 008 REV F i Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Program Mode Sequence of Operation Use the PROGRAM key to select the sequence s operation Operations that can be selected are Profile Increment Incr Decrement Decr Dispense Event Go To Pause Pump TTL Out Restart Stop When the required operation is displayed press the ENTER key Additional information may be requested Rate Enter the rate using the INFUSE RATE key to change units Note If the rate entered is invalid an error message will not be given at the immediate time of entry An OUT OF RANGE error message will be given during the running of the program Delta Rate Enter the rate of Increment or Decrement desired The units of the rate cannot be spec ified Units will be the same as the units of the current pumping rate at the time the sequence is executed Target Volume Enter the required delivered target volume of the sequence For increment and decre ment sequences the target volume is an increment
57. n A keypad is used for entry of operating data to the pump Data can also be entered via an RS 232 connector located on the rear panel The microcontroller using the internal Syringe Look Up Table calculates the cross sectional area of the syringe selected and calibrates the flow rate and volume accumulation The numerous features of the PHD 22 2000 series result from the use of microprocessor technology The PHD 22 2000 Infuse Withdraw model is equipped with all the functions of the PHD 22 2000 Infusion model along with additional features to provide the Withdraw Refill function The PHD 22 2000 Infuse Withdraw model s additional features include two additional function keys on the keypad REFILL RATE key and the INFUSE REFILL key and the addition of syringe Retaining Brackets and Adjustable Stops for refill Use of the REFILL RATE key the INFUSE REFILL key Retaining Brackets and Adjustable Stops are all described in detail in section 6 and 8 of this manual The PHD 22 2000 Programmable model is equipped with all the functions of the PHD 22 2000 Infuse Withdraw model along with additional features to provide Programmable functions The PHD 22 2000 Programmable models additional features include two additional function keys on the keypad the PROGRAM key and the AUTO FILL key Use of the PROGRAM key and the AUTO FILL key are described in detail in section 6 of this manual Publication 5400 008 REV F 3 Harvard Apparatus PHD 22 2000 Syrin
58. n the external event The triggered event causes an immediate con tinuation of the program at the specified sequence and the operation of the pump will be according to this sequence Data Specified Go To sequence number Go To Causes the program to immediately continue operation at the sequence specified Data Specified Go To sequence number Pause Pump stops for specified time then continues with next programmed sequence Current program rate set to 0 with no change in units Data Specified Time interval Pump Runs the pump continuously at the specified flow rate without any pumping target This mode can provide a background flow rate while waiting for an external event to trigger a new sequence specified by the EVENT operation Data Specified Flow rate Pumping direction Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Program Mode TTL Out Programmable TTL Pin TTL output Pin 4 of the user I O connector can be set to a HIGH or LOW level from within a program Data Specified TTL pin leve Restart Immediately restart program from the first sequence Data Specified None Stop Stops pump and the program terminates Data Specified None Program Printout If a printer is attached and the pump is stopped a program listing can be obtained by pressing the PROGRAM key for about one second PRINTING PROGRAM will be dis played while data is being sent
59. ng Pause interval pump stopped Pumping interrupted pump stopped Dispense trigger wait pump stopped 3 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Pump Chain Commands Pump Commands and Responses RUN STP DEL CLD Starts pumping according to the present setting of the pump If pump is already pumping a Not Applicable response will be given Stops pump if it was running If pump was already stopped a Not Applicable response will be given Request for volume delivered in ml Response is of the following format space space f f f f f f Request to zero volume delivered If the pump was interrupted it will can cel the interrupted condition If the pump is running request will not be accepted and a Not Applicable response will be given Otherwise no response is given RAT lt float gt lt units gt Request to set or query infusion rate setting Set infusion rate RAT rate Set infusion rate and units RAT rate units Rate is of format f f f f f f Units are one of Definition UM ul mn UH ul hr MM ml mn MH ml hr If rate is accepted and valid rate will become the new infusion rate If the rate is invalid an Out Of Range response will be given Command will not be accepted if the pump is running in the Program Mode and a Not Applicable response will be given Query infusion rate RAT Response is of the following format space spac
60. nty does not extend to damage result ing from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original consumer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow the exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus 84 October Hill Road Holliston Massachusetts 01746 using our toll free number 1 800 272 2775 or outside the U S call 508 893 8999 Email Address is bioscience harvardapparatus com Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs or replacement If the unit is replaced the replace ment unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state REPAIR FACILITIES
61. on model which is not equipped with retaining brackets or Adjustable Stops 9 Loosen the front and rear Refill Adjustable Stops 9 on the left side and slide them both to the right until they contact the Pusher Block 2 Then using the Allen Wrench tighten both Refill Adjustable Stops 9 and return the Allen Wrench to its holder With Multiple Syringe Holder Catalog Numbers MA1 70 2003 MA1 70 2006 MA1 70 2009 These Versions of the PHD 22 2000 have a saw tooth syringe holder capable of hold ing up to ten syringes emptying them simultaneously at identical rates This multiple syringe pump will accommodate up to Ten syringes of any size up to 10 ml e Six syringes of 20ml 100 ml Catalog Numbers MA1 70 2004 MA1 70 2007 MA1 70 2010 These Versions of the PHD 22 2000 have a multiple syringe holder which accommodates e Up to four syringes of 30ml to 140 ml To load syringes rotate the spring loaded retainer arm out of the way and lay syringes in V shaped slots with the syringe flange touching the holder Rotate the retainer arm to hold syringes in place If less than the maximum number of syringes are used try to place them symmetrically about the lead screw to present a balanced load Maximum Force The PHD 22 2000 will generate 50 Ibs of force With a 50 ml syringe it will deliver 25 psi Note The back pressure for each syringe will be divided by the number of syringes used Thus for four 50 ml syringes the maximum
62. ory 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any changes as they apply Do Not Dispose Product with Municipal Waste e Special Collection Disposal Required 5 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Table of Contents SUBJECT PAGE NO General Information Manual Description si dicisccccectveuterndanencncerextentxantedes 4 WairaNty crpsiernargo e a E 4 PUES casstt cose nyiges dacdt atin binnistori EN 4 Serial Numbers sosiedats cizciteceseeiaduticintbiesnisesaadataennn 4 ANID WAMONNG seriesas enera NRTA 4 General Safety Summary ssssssssnnnnnnnnnnnnnnnnnnnnnnn nnna 5 Technical SpecificationS sssssssnnnnnnnnnnnnnnnnnnn nanna 6 Theory of Operation ssssssssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nn nna 7 Features Pressure and Speed sccenstaccscieacecueeeueneieecteastecey 8 Built in Syringe Table and Custom Syringes 8 Infusion and Refill Rates csssssssesesererees 8 Target Vol ME sisca oora 8 PUIG FM E E T 8 Modes of Operation sicescictiecenccrrctiiecceecennae 8 External Connections sicsiccecciccresuensessenctiernonnarensacs 9 Nonvolatile Memory sscassnninsesinavenuctuctuntasiwarennainn 9 Stall Detection sssi ssireisinnsniis renieu niia 9 Visual Audible Alarm ccccccccccceceeeeeeeeeeeeeeeeeeees 9 Power Up OpWONS sciscsveinccianeccrmececstieitea
63. output connections are via a 9 pin D sub type connector All inputs are internally pulled up to a logic high Valve Control lt t _ Running output indicator User definable outputs Logic High Reference Foot Switch Timer Refill Event Figure 15 User I O Connector Specifications Pin Assignments 1 Vss Ground reference logic low Falling edge starts stops pump 2 Valve control output Low Infusing High Refilling 3 Running Output Indicator Low Pumped stopped High Pump running Programmable output Logic High Reference 5V 6 mA 6 Foot switch input Si Publication 5400 008 REV F 7 Timer Input Rising edge starts pump Falling edge stops pump 8 Directional control input Rising edge sets pump to infuse Falling edge sets pump to refill 9 Event input Falling edge triggers program event Logic Low OV 0 8V Max 6 mA current sink Logic High 2V 5V Max 6mA current source 2 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Appendix I Maintenance amp Troubleshooting Maintenance PHD 22 2000 series pumps require no special maintenance other than keeping them clean by avoiding accidental spills of pumped material The two guide rods and the lead screw should be sparingly lubricated periodically The guide rods and the lead screw should be lubricated with Magnalube G R grease Catalog MA1 0606 086 provided with the pump Solvents
64. pressure developed in each syringe will be 6 3 psi Syringe Loading Push Pull Mechanism 1 Loading syringes on the standard end see manual 2 Loading syringes on push pull end 1 ml through 30 ml only Remove 2 thumb nuts and syringe clamp Place syringes on moveable syringe holder Adjust syringe retainer using 2 set screws Position moveable holder for correct stroke Clamp on to guide rod with the T knob Lock stop plate in place with thumb nut Place syringe clamp over syringes and lock in place with thumb nut Publication 5400 008 REV F 5 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Operation Running the Pump Pressing the RUN STOP key starts the pump The pump will operate according to the rel evant data entered as interpreted by the selected run mode Pressing the RUN STOP key while the pump is running stops the pump and the right side of the top line of the display will indicate INTERRUPT plus gt for infusing or lt for refilling This indicates that the pumping operation has been suspended and can be continued Pressing the RUN STOP key again will continue the pumping operation at the point that it was interrupted Changing any of the settings including the pumping direction and the run mode cancels the interrupted operation and resets the display The pump can also be started and stopped from a remote source Diameter If the inside diameter of the syringe being used is known
65. pump receives its first command and all settings from when it was powered down will be recalled Stall Detection An optical detector is used to verify expected movement of the motor If the motor is prevented from turning due to jamming or excessive back pressure the pump will stop and the display will read PUMP STALLED Visual Audible Alarm After a power interruption the alarm can be selected to be a silent flashing display or an audible signal in addition to flashing display Refer to Section 6 for details on setting the Alarm Mode Power Up Options Pump Mode Only Enables the user to select between a standby mode waiting for a command to begin running after power is interrupted or a restart mode that begins running the pump at the settings that were in process when the power was interrupted Refer to Section 6 for details on setting the power up mode Program Storage Programmable model can store up to 4 sets of 9 program sequences for later selection Publication 5400 008 REV F 3 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual User Interface The user interface consists of a keypad with a display area The display consists of a 2 line 40 character alphanumeric vacuum fluorescent display The display will show one of three types of messages Default display setting display or an informational message The top line of the display is divided into three sections The first consists of the deliv ered volume
66. s according to a specified sequence of instructions Note All modes interact with Auto Fill Publication 5400 008 REV F S Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Features External Connections User I O Allows pump operations to be synchronized with external devices or by a person at a distance from the pump Connector pins are available to control direction of pump travel to control an external valve for refilling and for general use A simple contact clo sure to ground or TTL level signals may be used for inputs See Section 10 and Appendix H RS 232 Multiple pumps can be daisy chained together and remotely controlled from a com puter or any device communicating via RS 232 A scale can be connected enabling the pump to infuse by weight instead of by vol ume Section 10 A printer can be connected to record final volumes or weights whenever the pump stops In addition the program entered for the program mode can be listed on a con nected printer Both a scale and a printer may be connected simultaneously See Section 10 Remote Unit Catalog No s MA1 70 21xx and MA1 71 21xx Only Allows connection to the remote syringe pump unit The remote unit may be posi tioned up to 30 feet away from the control unit Nonvolatile Memory All operational data entered into the pump from the keypad or from a computer will be stored including the program On power up the display will blink until the
67. s and rate charts for use with older classic pumps Replacement Viton O Rings are available as are the more chem ically resistant Chemraz O Rings Luer Lock 1 4 inch 20 ml MA1 70 2251 MA1 70 2252 MA1 70 2253 MA1 70 2254 50 ml MA1 70 2255 MA1 70 2256 MA1 70 2257 MA1 70 2258 100 ml MA1 70 2259 MA1 70 2260 MA1 70 2261 MA1 70 2262 200 ml MA1 70 2263 MA1 70 2264 MA1 70 2265 MA1 70 2266 Replacement Tips Furnished with Sealing O Ring MA1 70 2247 Publication 5400 008 REV F MA1 70 2248 MA1 70 2249 MA1 70 2250 5 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Appendix B Stainless Steel Syringes Harvard High Pressure Small Volume Stainless Steel Syringes Both syringes and the fittings are constructed of 316 stainless steel The 8 ml syringe uses a pair of perfluoroelastomer o rings with Teflon back up rings When used with Harvard PHD 4400 high pressure pump working pressures of 1500 psi can be achieved great for chromatography and process control applications The 2 5 ml syringe utilizes a Bal Seal with a PEEK back up ring When used with a 4400 pump working pressures of 7000 psi can be achieved Specifications Volume 2 5 ml Dimensions Length of Barrel 16 9 cm 6 64 in Plunger Excursion 13 4 cm 5 28 in oD 1 3 cm 50 in ID 0 49 cm 191 in Max Test Pressure 9000 psi Working Pressure 7000 psi Cat No Product MA1 70 2267 8 ml Stainless Steel Syringe with
68. s interface allows all control information including a program to be entered into the pump from an external source such as a computer In addition this interface allows up to 100 pumps and in certain cases other RS 232 devices to be con trolled from a single RS 232 communication port on a computer Assign each pump in the pump chain a unique address from 0 to 99 This address is used to identify which pump is to receive a command and which pump is respond ing Configure each pump with its assigned address and the baud rate being used See Section 10 External Control amp Interfaces When the control device communicates with the pump a diamond appears on the far right of the display indicating that it is receiving RS 232 commands The diamond remains on the display until the pump is turned off or SET RS 232 is entered on the keyboard indicating a change in the RS 232 configuration A pump will not respond to pump chain communication while it is in a setting mode entered when user presses the SET key The pump can still be controlled from the keyboard while it is in a pump chain Control data that is changed via RS 232 will be stored in the pump s nonvolatile memory After each command is received and executed the pump terminates its responses with a prompt A prompt is a string of ASCII characters sent by a pump The PHD 22 2000 is capable of using two sets of pump interface commands This will allow users of Harvard Apparatus Model
69. ser I O Devices The pump does not need to be configured to attach a user I O device Simply plug the device into the 9 pin connector on the rear of the pump See Appendix I for wiring specifications Foot Switch or Relay Used to start and stop the pump Pressing the foot switch performs the same function as pressing the RUN STOP key on the keyboard The foot switch connector allows remote or automated operation of the pump Timer Opening the timer input starts the pump Closing the timer input stops the pump The timer input allows for an externally controlled pumping interval Pumping Direction not applicable for Infusion only models Sets the direction of pumping Opening the directional input sets the pump to infuse Closing the directional input sets the pump to refill The pumping direction input is recognized only in the situations that the INFUSE REFILL key would be recognized i e when the pump is stopped or running in the Pump Mode Valve Control not applicable for Infusion only models The valve control output is an indicator of the direction of pump travel When the out put is high the pump is set to Refill A low output indicates Infuse Publication 5400 008 REV F Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Programming Tutorial In the following examples the diameter is 26 7 mm and the infuse rate is 50 ml mn To run ATA 2 a program after entering it select Program mode 75 using the
70. t of the current rate of the pump or the infusion rate s units if first sequence Sequence is repeated the specified number of times Travel direction is as specified Data Specified Strategy 1 Delta flow rate Volume Time Interval 00 00 00 Volume increment Number of repetitions Pumping direction Strategy 2 Delta flow rate Time Time interval Number of repetitions Pumping direction Decr Same as INCR except rate is decremented Publication 5400 008 REV F 2 Harvard Apparatus PHD 22 2000 Syringe Pump Series User s Manual Program Mode Dispense Repeatedly dispense specified volume Runs at specified flow rate until a volume is pumped or a time interval has elapsed then pump will stop If no time interval was specified Strategy 1 the display will show TRIGGER and the next dispense will begin after an external or keyboard run command Otherwise the sequence will pause for specified time interval Sequence is repeated the specified number of times Travel direction is as specified Data Specified Strategy 1 Flow rate Volume Time Interval 00 00 00 Target volume Number of repetitions Pumping direction Strategy 2 Flow rate Time Target volume Time interval Number of repetitions Pumping direction Event Program Events A program event is an external event defined as a high to low tran sition on TTL pin 9 Within a program a one time event trigger can be set which watch es for and acts upo

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