Professional Plus Calibration Tips
Contents
1. Wait for the readings to stabilize and then press enter to accept the calibration If you receive a warning message stating that the calibration is questionable do not continue with the calibration Instead select No and investigate what is causing the questionable results If you accept a questionable calibration your ORP readings will be erroneous Typical causes for this error message include incorrect Sensor Port setup in the instrument a dirty sensor or bad calibration solution TROUBLESHOOTING TIPS Typical working life for ORP sensors is approximately 12 24 months depending on usage storage and maintenance Proper storage and maintenance generally extends the sensor s working life Clean and recondition the sensor if the sensor exhibits a slow response in Zobell solution i e it takes more than 90 seconds to stabilize when placed in Zobell If you get error messages during an ORP calibration check the following 1 2 Ensure the ORP calibration solution is good and not expired Ensure that the ORP sensor is installed in the correct port of the cable and the correct ISE is enabled in the Sensor Setup menu a If using an ORP or pH ORP combo sensor in a 6051020 cable ensure the sensor is installed in port 1 b If using an ORP sensor in a 60510 6051020 or 6051030 cable ORP should be enabled in ISE1 of the instrument s Sensor Setup menu c If using a pH ORP combo sensor in a 60510 6051020 or 6051030 cable ORP s2. and investigate what is causing the questionable results If you accept a questionable calibration your pH readings will be erroneous Typical causes for this error message include incorrect Sensor Port setup in the instrument a dirty sensor or bad buffer solution 13 After accepting a good calibration navigate to the GLP file and check the pH Slope and Slope of ideal A good slope should be between 55 and 60 mVs while the ideal is 59 mV If the slope drops below 53 the sensor should be reconditioned and recalibrated TROUBLESHOOTING TIPS Typical working life for pH sensors is approximately 12 24 months depending on usage storage and maintenance Proper storage and maintenance generally extends the sensor s working life Clean and recondition the sensor if a slow response in the field has been reported or if it takes more than 90 seconds to stabilize in pH buffer If you get an error message during a pH calibration check the following 1 Ensure the pH buffers are good and not expired 2 Ensure that the pH sensor is installed in the correct port of the cable and the correct ISE is enabled in the Sensor Setup menu a If using a pH or pH ORP combo sensor in a 6051020 cable ensure the sensor is installed in port 1 b If using a pH or pH ORP combo sensor in a 60510 6051020 or 6051030 cable pH should be enabled in ISE1 of the instrument s Sensor Setup menu c If using a pH sensor in a 6051010 or Quatro cable check to see if the
3. 10 0 ml of the above 100 mg L standard solution into a 1000 ml volumetric flask Add 2 6 g of lithium acetate dihydrate to the flask Add approximately 500 ml of distilled or deionized water swirl to dissolve the solid reagents and then dilute to the volumetric mark with water Mix well by repeated inversion and then transfer the 1 mg L standard to a storage bottle Add 3 drops of concentrated hydrochloric acid to the bottle then seal and agitate to assure homogeneity TROUBLESHOOTING TIPS Typical working life for ammonium sensors is approximately 3 6 months depending on use storage and maintenance Proper storage and maintenance generally extends the sensor s working life If you get error messages during an ammonium calibration check the following 1 Ensure the ammonium solutions are good and not expired 2 Ensure that the ammonium sensor is installed in the correct port of the cable and the correct ISE is enabled in the Sensor Setup menu a If using an ammonium sensor in a 6051020 cable ensure the sensor is installed in port 1 b If using an ammonium sensor in a 60510 6051020 or 6051030 cable ammonium should be enabled in ISE1 in the instruments Sensor Setup menu c If using an ammonium sensor in a 6051010 or Quatro cable check to see if the ammonium sensor is installed in the port 1 or port 2 If the ammonium sensor is installed in port 1 enable ammonium in ISE1 of the Sensor Setup menu If the ammonium sensor is installed in port 2
4. 2 8 weeks depending on use and storage In addition the membrane and electrolyte solution should be changed if a bubbles are visible under the membrane b significant deposits of dried electrolyte are visible on the membrane or c if the sensor shows unstable readings or other sensor related symptoms To install a new membrane cap follow these instructions 1 Remove the sensor guard or cal cup to access the sensor tip 2 Unscrew and remove any old membrane cap by holding the sensor when unscrewing the membrane cap Discard the used membrane cap 3 Thoroughly rinse the sensor tip with distilled or DI water 4 Fill anew membrane cap with the appropriate electrolyte solution that has been prepared according to the directions on the bottle Polarographic sensors use electrolyte that is in a white labeled bottle KCI Na2SOs Galvanic sensors use electrolyte that is in a blue labeled bottle NaCl Be very careful not to touch the membrane surface during this process Lightly tap the side of the membrane cap to release air bubbles that may be trapped 5 Thread the membrane cap onto the sensor It is normal for a small amount of electrolyte to overflow 12 Reconditioning the DO Sensor Anode Cathode Polarographic Sensors Model 605203 Due to the chemical reaction taking place under the membrane deposits will form on the gold cathode and silver anode The gold cathode will begin to appear dull and the silver anode
5. Temperature Sensor in a Quatro Cable 25 Cleaning a SENSOR POM ct Acct bepiikibezd A A a Ae nn OA ea A 26 Verifying Sensor Accuracy and CalibratiON ooooocccccccnccononoconoccccooooonnnrnn crono nono non nn n nn nono nn nnnnnnnnnnnnns 26 Resetting a Sensor to Factory DET ooo a RA AA AAA 26 Introduction This guide provides helpful instructions tips and troubleshooting suggestions for calibrating a Professional Plus instrument For more detailed information on calibration and information on how to setup and operate a Pro Plus please refer to the Pro Plus User Manual Calibration Worksheet The Calibration Worksheet on the following page is provided for your convenience Utilizing the Calibration Worksheet can help document your calibration and track the performance of your sensors Date of Calibration Technician Instrument Serial Number Software Revision Cable Model Number Temperature Reading Temperature Accurate Y N DO Sensor in use Polarographic Galvanic Sensor notated in Sensor menu Y N DO membrane changed Y N Color of Membrane Color notated in Sensor menu Y N Record the following calibration values Pre Cal After Cal Conductivity ORP DO True Barometric Pressure at time of calibration Pre Cal pH7 pH mV value Range 0 mV 50 mV pH4 pH mV value Range 165 to 180 from 7 buffer mV value pH 10 pH mV value Range 165 to 180 from 7 buffer mV value NOTE See pH Cal tips section for add
6. amount of potassium nitrate All other concentrations should remain unchanged It is important to note that some of these chemicals are hazardous and therefore the standards should only be prepared by qualified chemists in laboratories where proper safety precautions are possible It is the responsibility of the user to obtain and study the MSDS for each chemical and to follow the required instructions with regard to handling and disposal of these materials You will need Solid Potassium Nitrate or a certified 1000 mg l NO 3 N from a supplier Magnesium Sulfate high purity water good quality analytical balance 1000 ml volumetric flask accurate volumetric measuring devices for 100 ml 10 ml and 1 ml of solution and 1000 ml glass or plastic storage vessels 100 mg L standard Accurately weigh 0 7222 g of anhydrous potassium nitrate and transfer quantitatively into a 1000 ml volumetric flask Add 1 0 g of anhydrous magnesium sulfate to the flask Add approximately 500 ml of water to the flask swirl to dissolve all of the reagents and then dilute to the volumetric mark with distilled or deionized water Mix well by repeated inversion and then transfer the 100 mg L standard to a storage bottle Rinse the flask extensively with water prior to its use in the preparation of the 1 mg l standard Alternatively 100 mL of certified 1000 mg L NO 3 N standard can be used in place of the solid potassium nitrate 1 mg L standard Accurately measure 10 0 mL of t
7. be calibrated with up to three calibration points For highest accuracy perform a two point calibration with 1 and 100 mg L standards within 10 C of your sample temperature Rinse the sensors and cal cup with a small amount of ammonium solution 1 mg L for the first point and 100 mg L for the second point Fill the cup so that the ammonium sensor tip and the temperature sensor are submerged in solution If using a Quatro cable or 6051030 cable ensure that the conductivity sensor is also submerged in the calibration solution The salinity reading from the conductivity sensor is used in the algorithm for the ammonium measurement After entering the calibration screen change the calibration value if necessary If not using a conductivity sensor enter the salinity value of the calibration standard After calibration change the salinity correction value to the salinity value of the water you will be testing in the field in order to obtain the most accurate ammonium measurement You can change the salinity correction value in the Sensor menu As mentioned the Ammonium sensor should only be used in fresh water salinity lt 2 ppt The salinity of fresh water is typically O to 0 5 ppt Record the NH millivolts for each calibration point The acceptable mV outputs for each calibration solution are shown below NH 1 mg L 0 mV 20 mV new sensor only NH 100 mg L 90 to 130 mV gt 1 mg L mV value e The mV span between 1 mg L and 100 mg L value
8. complete reinstall the nitrate sensor and proceed with its calibration with no stabilization delay Or b Calibrate pH first immersing both sensors in the pH buffers After calibrating pH place the sensors in 100 mg L nitrate standard and monitor the reading Usually the reading starts low and may take awhile to reach a stable value When it does proceed with the calibration This may take several hours The nitrate sensor can be calibrated with up to three calibration points For highest accuracy perform a two point calibration with 1 and 100 mg L standards within 10 C of your sample temperature Rinse the sensors and cal cup with a small amount of nitrate solution 1 mg L for the first point and 100 mg L for the second point Fill the cup so that the nitrate sensor tip and the temperature sensor are submerged in solution If using a Quatro cable or 6051030 cable ensure that the conductivity sensor is also submerged in the calibration solution The salinity reading from the conductivity sensor is used in the algorithm for the nitrate measurement After entering the calibration screen change the calibration value if necessary If not using a conductivity sensor enter the salinity value of the calibration standard After calibration change the salinity correction value to the salinity value of the water you will be testing in the field in order to obtain the most accurate nitrate measurement You can change the salinity correction value in the Sens
9. connector be dry when installing removing or replacing sensors This will prevent water from entering the port Once a sensor is removed examine the connector inside the port If any moisture is present use compressed air to completely dry the connector or place directly in front of a steady flow of fresh air If you suspect port contamination follow the port cleaning procedures listed under Cleaning a Sensor Port Remove sensors upside down facing the ground to help prevent water from entering the port upon removal The instrument utilizes o rings as seals to prevent water from entering the sensor ports When the sensors are removed the o rings that provide the seal should be carefully inspected for contamination e g debris grit etc and cleaned if necessary If no dirt or damage to the o rings is evident wipe the o rings with a lint free cloth or lens cloth to remove the old o ring grease Then lightly apply new o ring grease provided in the maintenance kit to the o rings without removing them from their groove If there is any indication of damage the o ring should be replaced with an identical o ring At the time of o ring replacement the entire o ring assembly should be cleaned Do not over grease the o rings The purpose of the o ring grease it to keep the o ring in good condition Excess grease may collect grit particles that can compromise the seal Excess grease can also cause the waterproofing capabilities of the o ring to d
10. enable ammonium in ISE2 of the Sensor Setup menu Note If using with a pH or ORP sensor it is recommended to install the pH or ORP sensor in port 1 and the ammonium sensor in port 2 16 3 If using a 6051010 or Quatro cable you must have a sensor installed in port 1 for port 2 to operate Additionally ensure that the sensor installed in port 1 is in good working order In 6051010 and Quatro cables the sensors installed in port 1 and port 2 use the reference from the sensor installed in port 1 only Therefore if the sensor installed in port 1 is not working properly the readings from the sensor installed in port 2 will be erroneous as well 4 If you continue to get error messages during calibration clean the sensor 5 If you continue to get error messages during calibration soak the sensor in 100 mg L ammonium standard for several hours or overnight 6 If you suspect port contamination follow the instructions in the Cleaning a Sensor Port section 7 If you continue to have trouble calibrating the ammonium sensor contact your local YSI Representative or a YSI Authorized Service Center Cleaning the Ammonium Sensor The ammonium sensor uses a PVC membrane As always when handling a sensor care should be taken to avoid damaging the membrane After extensive use the membranes may become coated with a deposit or scoured with fine scratches which may cause a slow or reduced response low slope or unstable readings Deposits may be rem
11. in good working order In 6051010 and Quatro cables the sensors installed in port 1 and port 2 use the reference from the sensor installed in port 1 only Therefore if the sensor installed in port 1 is not working properly the readings from the sensor installed in port 2 will be erroneous as well 4 Ifyou continue to get error messages during calibration clean the sensor 5 If you continue to get error messages during calibration soak the sensor in 1000 mg L chloride standard for several hours or overnight 6 If you suspect port contamination follow the instructions in the Cleaning a Sensor Port section 7 If you continue to have trouble calibrating the chloride sensor contact your local YSI Representative or a YSI Authorized Service Center 22 Cleaning and Reconditioning the Chloride Sensor The chloride sensor is considered a pellet membrane ISE As always when handling sensors care should be taken to avoid damaging the membrane This sensor can be regenerated by washing with alcohol and or gently polishing with fine emery paper in a circular motion to remove any deposits or discoloration then thoroughly washing with deionized water to remove any debris The sensor may require soaking in the high chloride calibration solution to recover its performance Soak in 1000 mg L for several hours or overnight 23 Installing and Uninstalling Sensors GENERAL PRECAUTIONS It is important that the entire sensor connector and cable
12. low slope or unstable readings Deposits may be removed with a fine jet of deionized water or rinsing in alcohol followed by soaking in 100 mg L nitrate calibration standard The sensor may require soaking in the high nitrate calibration solution to recover its performance Soak in 100 mg L for several hours or overnight 20 Chloride The chloride sensor should be calibrated every day the instrument is used The chloride sensor should only be used in fresh water salinity lt 2 ppt and to depths of 55 feet 17 meters of water CALIBRATION TIPS 1 10 11 12 If using a chloride sensor with either a pH or ORP sensor on a 6051010 or Quatro cable install the pH or ORP sensor in port 1 and the chloride sensor in port 2 If using a chloride sensor on a 6051010 or Quatro cable calibrate the sensor in port 1 prior to calibrating the sensor in port 2 The sensor in port 2 uses the reference of the sensor installed in port 1 Therefore it is important to verify that the sensor in port 1 is working properly before calibrating the sensor in port 2 See Chloride Troubleshooting Tips for additional information on port configuration Exposure to the high ionic content of pH buffers can cause a significant but temporary drift in the chloride sensor Therefore if calibrating a pH sensor either c Remove the chloride sensor from the cable bulkhead and plug the port After pH calibration is complete reinstall the chloride sensor and p
13. to the volumetric mark with water Mix well by repeated inversion and then transfer the 10 mg L standard to a storage bottle TROUBLESHOOTING TIPS Typical working life for chloride sensors is approximately 3 6 months depending on use storage and maintenance Proper storage and maintenance generally extends the sensor s working life If you get error messages during a chloride calibration check the following 1 Ensure the chloride solutions are good and not expired 2 Ensure that the chloride sensor is installed in the correct port of the cable and the correct ISE is enabled in the Sensor Setup menu a If using a chloride sensor in a 6051020 cable ensure the sensor is installed in port 1 b If using a chloride sensor in a 60510 6051020 or 6051030 cable chloride should be enabled in ISE1 of the instrument s Sensor Setup menu c If using a chloride sensor in a 6051010 or Quatro cable check to see if the chloride sensor is installed in port 1 or port 2 If the chloride sensor is installed in port 1 enable chloride in ISE1 of the Sensor Setup menu If the chloride sensor is installed in port 2 enable chloride in ISE2 of the Sensor Setup menu Note If using with a pH or ORP sensor it is recommended to install the pH or ORP sensor in port 1 and the chloride sensor in port 2 3 If using a 6051010 or Quatro cable you must have a sensor installed in port 1 for port 2 to operate Additionally ensure that the sensor installed in port 1 is
14. BEEBEBRA gt Sa mee NN Calibration Tips Table of Contents ROUCO ar AAA AAA A EA AAA VNR OTST ad add kazda aaa Andel n 1 Calibration Worksheet 5 soud nd d a 1 AAA aah cd aie ten a dua ak sll O 3 Galibration TIPS a A AAA EE A AE AA sad 3 Troubleshootn TDS cmd da cita 3 CONdUCHVIY ze o o eo 4 Calibration NDS cessed A a n nar AA A Ah 4 FFOUDSSHOOUN MIOS tarda 4 DA DA A EA SAA AAA EEN SA 6 Calibration TIOS AA AA AA AAA AA RA AR AR 6 Troubleshooting TIPS A asain pou kos on 6 ORP O n O E n E ee lo jooo bh rob 9 Calibration VIDS aeeie ico AS AE Aa EUA 9 Tro bleshooting MOS dad 9 BES A rc ae Sac ae ae eae Bas Sealers heel ras eta 11 Calibration TIPS 22d A AA AAA AS AA AS AA AAA ATAN 11 Troubleshooting PS diana dna unie nooo udasdnoud s 12 ARMONIA AAA ar tenner Aa VLEK AE AAA V KA A oto RAS K 15 Calibration UMS ape ai a de tl O AAA AA A AAA 15 Troubleshooting TIPS A zac AER 16 A IR A 18 Calibration TIPS ENANA G L G L T aS 18 TrOUbleSNOQUAO PS dada rios hodl 19 A A A AT O ce 21 Calibration TIPS adnie DOE AS EA ERA ERA AE nd Frew er 21 Troubleshooting n 22 Installing and Uninstalling Sensors 110 bs 24 General Pr cautions A as tau data tata AE E E au set na 24 Uninstalling DO pH ORP pH ORP and ISE Sensors eee 25 Installing DO pH ORP pH ORP and ISE Sensors eee eee een 25 Uninstalling a Conductivity Temperature Sensor in a Quatro Cable 25 Installing a Conductivity
15. The cleaning can be chemical and or mechanical Removing the sensor from the cable may make cleaning easier Initially moisten a soft clean cloth lens cleaning tissue or cotton swab to remove all foreign material from the glass bulb and or platinum button Then use a moistened cotton swab to carefully remove any material that may be blocking the reference electrode junction of the sensor CAUTION When using a cotton swab be careful NOT to wedge the swab between the guard and the glass sensor If necessary remove cotton from the swab tip so that the cotton can reach all parts of the sensor tip without stress You can also use a pipe cleaner for this cleaning if more convenient If good pH and or ORP response is not restored perform the following additional procedure 1 Soak the sensor for 10 15 minutes in clean water containing a few drops of commercial dishwashing liquid 2 GENTLY clean the glass bulb and platinum button by rubbing with a cotton swab soaked in the cleaning solution 3 Rinse the sensor in clean water wipe with a cotton swab moistened with clean water and then re rinse with clean water If good pH and or ORP response is still not restored perform the following additional procedure 1 Soak the sensor for 30 60 minutes in one molar 1 M hydrochloric acid HCI This reagent can be purchased from most lab supply distributors Be sure to follow the safety instructions included with the acid 2 Rinse the sensor in clean wa
16. The conductivity calibration should be verified every day the instrument is used However the conductivity sensor is very stable and may hold its calibration for several weeks CALIBRATION TIPS 1 It is not necessary to calibrate conductivity specific conductance and salinity Calibrating one of these parameters will simultaneously calibrate the others YSI recommends calibrating specific conductance temperature compensated conductivity for greatest ease and accuracy Ensure the conductivity sensor is clean and dry before performing a specific conductance calibration Always use fresh traceable conductivity calibration solution when calibrating the conductivity sensor a The shelf life of conductivity solution is one month after being opened This is due to potential changes in the value of the solution caused by evaporation which can occur after opening the bottle Be sure to write the open date on the bottle so you know that you are using good calibration solution b Never calibrate with a conductivity solution that is less than 1 0 mS cm You are setting the slope on a linear device so a good strong conductivity signal will give you the best performance Use 1 0 mS cm for fresh water 10 mS cm for brackish to estuarine water and 50 mS cm for salt water 1 0 mS millisiemens 1000 uS microsiemens Pre rinse the cal cup and sensors with a small amount of calibration standard or rinse standard and discard When calibrating the conduct
17. alues should be less than 100 mVs If the difference is 80 mVs or higher consider replacing the sensor as it is nearing the end of its life span 10 Dissolved Oxygen The dissolved oxygen sensor should be calibrated every day the instrument is used It is not necessary to calibrate in both and mg L or ppm Calibrating in will simultaneously calibrate mg L and ppm and vice versa CALIBRATION TIPS 1 The Pro Plus can be calibrated in air saturated water water saturated air or against a Winkler Titration You can perform a 1 or 2 point DO calibration A 2 point calibration includes 1 point in a zero oxygen environment and the 2 point at full saturation For both ease of use and aceuracy YSI recommends that you perform a 1 point calibration in water saturated air Make sure that there is a good membrane with fresh electrolyte O2 probe solution installed on the DO sensor The membrane should be clean and free of wrinkles There should not be any air bubbles present under the membrane Membranes should be changed regularly and generally last 2 8 weeks depending on use and storage To perform a 1 point calibration in water saturated air place the sensor in a 100 humid environment This can be accomplished several ways a For the 60520 and 6052030 cables moisten the sponge in the gray calibration sleeve with a small amount of clean water and place it over the sensor guard b For the 6051020 and Quatro cables place a small am
18. anding disk and twist it in a circular motion to sand the gold cathode The goal is to sand off any build up and to lightly scratch the cathode to provide a larger surface area for the electrolyte solution under the membrane Usually 3 to 4 twists of the sanding disk are sufficient to remove deposits and for the gold to appear to have a matte finish Rinse thoroughly and wipe the gold cathode with a wet paper towel before putting on a new membrane cap Note Be sure to 1 Only use fine 400 grit wet dry sand paper and 2 Sand as mentioned in the above procedures Not adhering to either of these instructions can damage the electrodes If this procedure is unsuccessful as indicated by improper DO sensor performance contact your local YSI Representative or a YSI Authorized Service Center Galvanic Sensors Model 605202 The Galvanic dissolved oxygen sensor is continuously reducing oxygen even when the Pro Plus is turned off This factor allows the sensor to be used with no warm up time as soon as the instrument is powered on However because the sensor is on all the time some solid from the oxidation of the zinc anode will form in the electrolyte within 1 2 weeks of activation The Galvanic electrolyte solution will appear milky white after use but this will not affect the accuracy of the sensor unless there is excessive build up which may result in jumpy readings Otherwise the color change is acceptable and normal as long as DO readings rema
19. at some of the chemicals reguired for these solutions could be hazardous under some conditions It is the responsibility of the user to obtain and study the MSDS for each chemical and to follow the reguired instructions with regard to handling and disposal of these chemicals You will need Solid sodium chloride or a certified 1000 mg L chloride solution from a supplier magnesium sulfate high purity water a good quality analytical balance 1000 ml volumetric flask an accurate 10 ml measuring devices and 1000 ml glass or plastic storage vessels 1000 mg L standard Accurately weigh 1 655 grams of anhydrous sodium chloride and transfer into a 1000 ml volumetric flask Add 0 5 grams of anhydrous magnesium sulfate to the flask Add 500 ml of distilled or deionized water to the flask swirl to dissolve all of the reagents and then dilute to the volumetric mark with water Mix well by repeated inversion and then transfer the 1000 mg L standard to a storage bottle Rinse the flask extensively with water prior to its use in the preparation of the 10 mg L standard Alternatively simply add 0 5 grams of magnesium sulfate to a liter of a 1000 mg L chloride standard from a certified supplier 10 mg L standard Accurately measure 10 ml of the above 1000 mg L standard solution into a 1000 ml volumetric flask Add 0 5 grams of anhydrous magnesium sulfate to the flask Add 500 ml of distilled or deionized water swirl to dissolve the solid reagents and then dilute
20. d calibration navigate to the GLP file and check the conductivity cell constant for the calibration For highest accuracy the cell constant should be 5 0 0 5 However the acceptable range is 5 1 0 A cell constant outside of this range indicates that a questionable calibration was accepted TROUBLESHOOTING TIPS If you get an error message during calibration be sure that you are IRUN Entering the correct calibration value 1 mS cm 1000 uS cm Calibrating in Specific Conductance mode Using enough solution to cover the vent holes on the sensor Dislodging any air bubbles that could be trapped in the sensor Using a fresh traceable conductivity calibration solution If you are following the above recommendations and still receiving an error message check the conductivity sensor to make sure it is clean A clean conductivity sensor should read less than 3 uS cm in dry air If your sensor is dry and giving you a reading higher than 3 uS cm in air it should be cleaned The conductivity calibration generates its cell constant value after calibration The ideal cell constant is 5 0 0 5 but 5 0 1 0 is acceptable Any significant jump or change in this number from one calibration to the next usually indicates a problem with the calibration and or sensor If you are sure that your calibration standard is good and your calibration process is correct then your sensor may need to be cleaned Cleaning the Conductivity Sensor Th
21. e openings that allow sample access to the conductivity electrodes should be cleaned regularly The small cleaning brush included in the Maintenance Kit is intended for this purpose Dip the brush in clean water and insert it into each hole 10 to 12 times In the event that deposits have formed on the electrodes it may be necessary to use a mild detergent laboratory grade soap or bathroom foaming tile cleaner with the brush Rinse thoroughly with clean water then check the response and accuracy of the conductivity sensor with calibration solution Quatro Cables Quatro cables have a replaceable combination conductivity temperature sensor p n 5560 All other Pro Plus cables have integral conductivity sensors If using a Quatro cable and your conductivity sensor is not calibrating or is reading gt 3 uS cm in dry air after being cleaned remove the conductivity temperature sensor from the cable The Pro Plus should read lt 3 uS cm for conductivity not specific conductance without a conductivity sensor installed If the instrument is reading gt 3 uS cm without a sensor installed the conductivity temperature port on the cable may be contaminated Refer to the Cleaning the Sensor Port section of this document for information on how to clean the port If the conductivity measurement continues to read more than 3 uS cm without a conductivity temperature sensor installed there may be a problem with the cable and or instrument In this case contact y
22. e to properly seat the sensor may result in damage Twist the sensor clockwise to engage threads and finger tighten Do not use a tool This connection is waterproof Please refer to the sensor installation sheet that is included with each sensor for detailed instructions UNINSTALLING A CONDUCTIVITY TEMPERATURE SENSOR IN A QUATRO CABLE First ensure that the entire sensor and cable bulkhead are clean and dry Remove sensors upside down facing the ground to help prevent water from entering the port upon removal Remove the conductivity temperature sensor using the installation tool to loosen the stainless steel retaining nut Insert the tool into one of the holes in the stainless steel retaining nut Next use the installation tool to turn the stainless steel retaining nut counter clockwise to loosen Do not allow the sensor to be turned with the tool Turning the sensor with the tool will likely damage the sensor connector Once the stainless steel retaining nut has been completely loosened from the bulkhead remove the sensor from the bulkhead by pulling the sensor straight out of the port INSTALLING A CONDUCTIVITY TEMPERATURE SENSOR IN A QUATRO CABLE First ensure both the sensor connector and sensor port on the cable are clean and dry If any moisture is present use compressed air to completely dry the connector or place directly in front of a steady flow of fresh air If you suspect port contamination follow the port cleaning procedures li
23. erature sensor can not be calibrated nor should calibration be required TROUBLESHOOTING TIPS If the temperature sensor is not reading accurately ensure that it is clean and free of debris The conductivity cleaning brush and warm water with mild detergent can be used to scrub the temperature sensor if needed Alternatively you can use a toothbrush to clean the sensor Quatro Cables Quatro cables have a replaceable combination conductivity temperature sensor p n 005560 All other Pro Plus cables have integral temperature sensors If using a Quatro cable and your temperature sensor is not reading accurately remove the conductivity temperature sensor from the cable The Pro Plus should read C without a temperature sensor installed If the instrument is reading any other value the conductivity temperature port on the cable may be contaminated Refer to the Cleaning the Sensor Port section of this document for information on how to clean the port After cleaning the port recheck the temperature reading If the temperature reading is still not displaying C without the sensor installed there may be a problem with the cable and or instrument In this case contact your local YSI Representative or a YSI Authorized Service Center Other Pro Plus Cables If your temperature sensor is not reading accurately after cleaning around the sensor contact your local YSI Representative or an YSI Authorized Service Center Conductivity
24. fter performing a chemical cleaning perform a mechanical cleaning on both the anode and cathode Mechanical cleaning In order to sand the silver anode along the shaft of the sensor remove the membrane and hold the sensor in a vertical position Wet 400 grit wet dry sand paper with a small amount of clean water then gently wrap it around the sensor anode and twist it a few times to lightly sand the anode the goal is to sand off any build up without scratching or removing layers of the anode itself Usually 3 to 4 twists of the sanding disk are sufficient to remove deposits However in extreme cases more sanding may be required to remove all of the deposits After completing the sanding procedure repeatedly rinse the electrode with clean water and wipe with lens cleaning tissue to remove any grit left by the sanding disk Thoroughly rinse the entire tip of the sensor with distilled or deionized water and install a new membrane 13 Gold Cathode For correct sensor operation the gold cathode must be textured properly It can become tarnished or plated with silver after extended use Never use chemicals or abrasives not recommended or supplied by YSI First dry the sensor tip completely with lens cleaning tissue Wet 400 grit wet dry sand paper with a small amount of clean water and place it face up in the palm of your hand Next with your free hand hold the sensor in a vertical position tip down Place the sensor tip directly down on the s
25. he above 100 mg L standard solution into a 1000 mL volumetric flask Add 1 0 g of anhydrous magnesium sulfate to the flask Add approximately 500 mL of distilled or deionized water swirl to dissolve the solid reagents and then dilute to the volumetric mark with water Mix well by repeated inversion and then transfer the 1 mg L standard to a storage bottle Recipes are given for 1 and 100 mg L Other concentrations can be made by altering the amount of potassium nitrate All other concentrations should remain unchanged TROUBLESHOOTING TIPS Typical working life for nitrate sensors is approximately 3 6 months depending on use storage and maintenance Proper storage and maintenance generally extends the sensor s working life If you get error messages during a nitrate calibration check the following 1 Ensure the nitrate solutions are good and not expired 2 Ensure that the nitrate sensor is installed in the correct port of the cable and the correct ISE is enabled in the Sensor Setup menu a If using a nitrate sensor in a 6051020 cable ensure the sensor is installed in port 1 b If using a nitrate sensor in a 60510 6051020 or 6051030 cable nitrate should be enabled in ISE1 of the instrument s Sensor Setup menu c If using a nitrate sensor in a 6051010 or Quatro cable check to see if the nitrate sensor is installed in port 1 or port 2 If the nitrate sensor is installed in port 1 enable nitrate in ISE1 of the Sensor Setup menu If
26. he chloride sensor should only be used in fresh water salinity lt 2 ppt The salinity of fresh water is typically O to 0 5 ppt Record the Cl millivolts for each calibration point The acceptable mV outputs for each calibration solution are shown below CI 10 mg L 225 mV 20 mV new sensor only Cl 1 000 mg L 80 to 130 mV lt 10 mg L mV value e The mV span between 10 mg L and 1000 mg L values should be 80 to 130 mV The slope should be 40 to 65 mV per decade Wait for the chloride and temperature readings to stabilize in each calibration solution and then press enter to accept each calibration point Rinse the sensor and cal cup between calibration points with a small amount of the next buffer After pressing enter to accept your last calibration point press cal to complete the calibration Otherwise you will continue calibrating up to 3 calibration points If you receive a warning message stating that the calibration is questionable do not continue with the calibration Instead select No and investigate what is causing the questionable results If you accept a questionable calibration your chloride readings will be erroneous Typical causes for this error message include incorrect Sensor Port setup in the instrument a dirty sensor or bad buffer solution 21 Preparing Chloride Calibration Solution The following recipes are provided for preparation of 10 and 1000 mg L chloride reagents It is important to note th
27. hould be enabled in ISE2 of the instruments Sensor Setup menu d If using an ORP sensor in a 6051010 or Quatro cable check to see if the ORP sensor is installed in port 1 or port 2 If the ORP sensor is installed in port 1 enable ORP in ISE1 of the Sensor Setup menu If the ORP sensor is installed in port 2 enable ORP in ISE2 of the Sensor Setup menu If using a pH ORP combo sensor in a 6051010 or Quatro cable ORP will not be measured or reported If using a 6051010 or Quatro cable you must have a sensor installed in port 1 for port 2 to operate Additionally ensure that the sensor installed in port 1 is calibrated and in good working order In 6051010 and Quatro cables the sensors installed in port 1 and port 2 use the reference from the sensor installed in 9 port 1 only Therefore if the sensor installed in port 1 is not working properly the readings from the sensor installed in port 2 will be erroneous If you continue to get error messages during calibration clean and recondition the sensor per the instructions in the pH Troubleshooting section of this document If you suspect port contamination follow the instructions in the Cleaning a Sensor Port section before reinstalling the sensor If you continue to have problems you can check the offset of the ORP sensor by performing a factory reset to the ORP sensor After resetting the sensor compare the ORP mV readings in Zobell solution to the calibration value The difference between v
28. iminish potentially causing leaks If excess grease is present remove it using a lens cloth or lint free cloth To remove the o rings Use a small flat bladed screwdriver or similar blunt tipped tool to remove the o ring from its groove Do not use a sharp object to remove the o rings Using a sharp object could damage the o ring groove which would allow water to enter the port resulting in permanent damage to the port and sensor Check the o ring and the groove for any excess grease or contamination If contamination is evident clean the o ring and nearby plastic parts with lens cleaning tissue or equivalent lint free cloth Alcohol can be used to clean the plastic parts but use only water and mild detergent on the o ring itself Using alcohol on o rings may cause a loss of elasticity and may promote cracking Also inspect the o rings for nicks and imperfections Before re installing the o rings make sure to use a clean workspace clean hands and avoid contact with anything that may leave fibers on the o ring or grooves Even a very small amount of contamination hair grit etc may cause a leak To re install the o rings Place a small amount of o ring grease between your thumb and index finger Draw the o ring through the grease while pressing the fingers together to place a very light covering of grease to the o ring Place the o ring into its groove making sure that it does not twist or roll Do no excessively stretch the o ring during i
29. in stable The rate of solid formation is dependent on the type of membrane installed The formation of solids typically form more rapidly with the 5912 black 1 mil Teflon less rapid with 5913 yellow 1 25 mil PE and least rapid with 5914 blue 2 mil PE When changing the membrane rinse the anode and cathode with distilled or deionized water and wipe with a clean paper towel If white deposits are evident on the anode after rinsing and wiping remove the deposits by sanding the anode with 400 grit wet dry sand paper following the Mechanical Cleaning instructions under the Polarographic Silver Anode maintenance section If there are deposits on the cathode sand the cathode with 400 grit wet dry sand paper following the maintenance instructions listed for the Polarographic Gold Cathode Note Do not perform the Polarographic chemical cleaning on a Galvanic sensor If this procedure is unsuccessful as indicated by improper sensor performance contact your local YSI Representative or a YSI Authorized Service Center 14 Ammonium The ammonium sensor should be calibrated every day the instrument is used The ammonium sensor should only be used in fresh water salinity lt 2 ppt and to depths of 55 feet 17 meters of water Ammonia is calculated from the ammonium temperature and pH readings pH greatly affects the ammonia calculation Therefore for highest accuracy in the ammonia calculation be sure to use a pH sensor in conjunc
30. itional information Span between pH 4 and 7 and 7 and 10 mV values should be 165 to 180 mV 177 is the ideal distance or 59 mV per pH unit Ammonium 1 point 1 mg L NH4 mV value Range 0 mV 20 mV new sensor only 2 point 100 mg L NH4 mV value Range 90 to 130 mV gt 1 mg L mV value Nitrate 1 point 1 mg L NO3 mV value Range 200 mV 20 mV new sensor only 2 point 100 mg L NO3 mV value Range 90 to 130 mV lt 1 mg L mV value Chloride 1 point 10 mg L Cl mV value Range 225 mV 20 mV new sensor only 2 point 1000mg L Cl mV value Range 80 to 130 lt 10 mg L mV value Record the following diagnostic numbers after calibration by viewing the glp file and reading the values for the day s calibration Conductivity Cal Cell Constant Range 5 0 1 0 acceptable DO Sensor Value uA Membrane dependent see DO Cal Tips pH Slope 55 to 60 mV pH 59 ideal pH Slope of ideal Temperature CALIBRATION TIPS Before calibrating any other Pro Plus sensor verify that the temperature sensor is reading accurately by comparing it to a traceable thermometer or other known reference in a water bath Temperature compensation is used in every other sensor measurement so its accuracy should be verified and recorded each time the Pro Plus is calibrated Be sure to consider the specification tolerances of both the Pro Plus temperature sensor and the thermometer when comparing the measurements The Pro Plus temp
31. ivity sensor the calibration solution must cover the top vent holes of the conductivity sensor If using a Quatro cable the top vent hole is located on the side of the combination conductivity temperature sensor If using a different cable the conductivity sensor is integral to the cable and the sensor has two vent holes located close to the cable Ensure the entire conductivity sensor is submerged in the solution or the instrument will read approximately half the expected value After placing the sensor into the solution gently move the sensor up and down to remove any air bubbles that may be trapped in the conductivity sensor If calibrating Specific Conductance enter the value of the conductivity solution as it is listed for 25 C Make sure you are entering the correct units 1 mS 1 000 uS If you receive a warning message stating that the calibration is questionable do not continue with the calibration Instead select No and investigate what is causing the questionable results If you accept a questionable calibration your conductivity readings and your DO mg L readings will be erroneous Typical causes for this error message include incorrect entries entering 1000 uS cm instead of 1 0 mS cm not using enough solution to cover the vent holes air bubbles trapped in the sensor calibrating in conductivity instead of specific conductance dirty conductivity electrodes and or bad calibration solution After accepting a goo
32. ly 0 0 5 ppt and seawater is typically 35 ppt After accepting the calibration navigate to the GLP menu and record the DO sensor s value sensor current in uA The acceptable sensor currents when calibration is performed at 25 C in a 100 saturated air environment at 760 mmHg are 1 25 mil PE membrane yellow membrane Average 6 15 uA min 4 31 UA max 8 00 UA 2 0 mil PE membrane blue membrane Average 3 38 uA min 2 37 uA max 4 40 uA 1 mil Teflon membrane Average 16 29 uA min 11 40 uA max 21 18 uA If you receive a warning message stating that the calibration is questionable do not continue with the calibration Instead select No and investigate what is causing the questionable results If you accept a questionable calibration your DO readings will be erroneous Typical causes of a calibration error message include incorrect sensor membrane or port setup in the instrument incorrect barometric pressure information a bad membrane or a sensor that needs reconditioned TROUBLESHOOTING TIPS 1 Ensure that the correct sensor type and membrane type are enabled in the Sensor Setup Menu Galvanic sensors have a gray probe body and Polarographic sensors have a black probe body If using a 6051020 cable ensure that the DO sensor is installed in port 2 If using a Quatro cable ensure that the DO sensor is installed in the port labeled DO Ensure the Pro Plus barometer is reading accurately The DO Saturation calibration
33. nstallation Use your grease coated finger to once again lightly go over the mating surface of the o ring Do not over grease the o rings The excess grease may collect grit particles that can compromise the seal Excess grease can also cause the waterproofing capabilities of the o ring to diminish potentially causing leaks If excess grease is present remove it using a lens cloth or lint free cloth 24 UNINSTALLING DO PH ORP PH ORP AND ISE SENSORS First ensure that the entire sensor and cable bulkhead are clean and dry Remove sensors upside down facing the ground to help prevent water from entering the port upon removal Simply unscrew the sensor from the cable by holding the sensor port end of the cable bulkhead in one hand and the sensor in the other hand Twist the sensor counter clockwise to unscrew the sensor from the port INSTALLING DO PH ORP PH ORP AND ISE SENSORS First ensure both the sensor connector and sensor port on the cable are clean and dry If any moisture is present use compressed air to completely dry the connector or place directly in front of a steady flow of fresh air If you suspect port contamination follow the port cleaning procedures listed under Cleaning a Sensor Port To connect the sensor grasp the sensor with one hand and the sensor port end of the cable bulkhead in the other Push the sensor into the connector on the cable until it is properly seated and only one o ring is visible Failur
34. or menu As mentioned the nitrate sensor should only be used in fresh water salinity lt 2 ppt The salinity of fresh water is typically 0 to 0 5 ppt Record the NO millivolts for each calibration point The acceptable mV outputs for each calibration solution are shown below NO3 1 mg L 200 mV 20 mV new sensor only NO3 100 mg L 90 to 130 mV lt 1 mg L mV value e The mV span between 1 mg L and 100 mg L values should be 90 to 130 mV The slope should be 45 to 65 mV per decade Wait for the nitrate and temperature readings to stabilize in each calibration solution and then press enter to accept each calibration point Rinse the sensor and cal cup between calibration points with a small amount of the next buffer you will continue calibrating up to 3 calibration points If you receive a warning message stating that the calibration is questionable do not continue with the calibration Instead select No and investigate what is causing the questionable results If you accept a questionable calibration your nitrate readings will be erroneous Typical causes for this error message include incorrect Sensor Port setup in the instrument a dirty sensor or bad buffer solution 18 Preparing Nitrate Calibration Solution We recommend using YSI calibration solutions whenever possible However qualified users can save cost by following these recipes for 1 and 100 mg L nitrate standards Other concentrations can be made by altering the
35. ount of water in the calibration storage cup and place it over the sensors When screwing the calibration cup onto the sensor bulkhead only engage one or two threads Do not screw the calibration cup completely onto the sensor bulkhead The goal is to have air exchange between inside and outside the calibration cup The sponge and calibration sleeve cup should be clean since bacterial growth may consume oxygen and interfere with the calibration Be sure the sensor is in air not water and that there are not any water droplets on the membrane or temperature sensor After entering the calibration mode wait approximately 5 to 15 minutes for the storage container to become completely saturated and if using a polarographic sensor to allow the sensor to stabilize Salinity affects the ability of water to hold oxygen and is used by the instrument to calculate DO mg L ppm The Salinity value displayed near the top of the DO calibration screen is either the salinity correction value entered in the Sensor menu or the Salinity value as measured by the conductivity sensor in use If you are using a conductivity sensor ensure that it is calibrated and reading correctly in order to obtain accurate DO mg L ppm measurements If you are not using a conductivity sensor the Salinity correction value should be the salinity of the water you will be testing Highlight Salinity and press enter to modify this setting if necessary The salinity of fresh water is typical
36. our local YSI Representative or a YSI Authorized Service Center Other Pro Plus Cables If your conductivity sensor is not calibrating or is reading gt 3 uS cm in dry air after performing a sensor cleaning contact your local YSI Representative or a YSI Authorized Service Center pH The pH calibration should be verified every day the instrument is used However a new pH sensor may be capable of holding its calibration for several days CALIBRATION TIPS 1 If using a pH sensor in a 6051010 or Quatro cable calibrate the sensor in port 1 prior to calibrating the sensor in port 2 The sensor in port 2 uses the reference of the sensor installed in port 1 Therefore it is important to verify that the port 1 sensor is working properly before calibrating the port 2 sensor See pH Troubleshooting Tips for additional info 2 The pH sensor can be calibrated with up to six calibration points 3 Calibration can be accomplished in any buffer order 4 pH7 buffer should be used regardless of how many calibration points you use however it does not have to be the first point 5 In most cases a two point calibration is all that is reguired 4 and 7 or 7 and 10 You can bracket the expected in situ pH values Use a three point calibration with 4 7 and 10 if the in situ pH values are unknown or if you expect the in situ values to be on both sides of the pH scale 6 Rinse the sensors and cal cup with a small amount of pH buffer Fill the cup
37. oved with a fine jet of deionized water or rinsing in alcohol followed by soaking in 100 mg L ammonium calibration standard The sensor may require soaking in the high ammonium calibration solution to recover its performance Soak in 100 mg L for several hours or overnight Nitrate The nitrate sensor should be calibrated every day the instrument is used The nitrate sensor should only be used in fresh water salinity lt 2 ppt and to depths of 55 feet 17 meters of water CALIBRATION TIPS 1 10 After pressing enter to accept your last calibration point press cal to complete the calibration Otherwise 11 12 If using a nitrate sensor with either a pH or ORP sensor on a 6051010 or Quatro cable install the pH or ORP sensor in port 1 and the nitrate sensor in port 2 If using a nitrate sensor on a 6051010 or Quatro cable calibrate the sensor in port 1 prior to calibrating the sensor in port 2 The sensor in port 2 uses the reference of the sensor installed in port 1 Therefore it is important to verify that the sensor in port 1 is working properly before calibrating the sensor in port 2 See nitrate Troubleshooting Tips for additional information on port configuration Exposure to the high ionic content of pH buffers can cause a significant but temporary drift in the nitrate sensor Therefore if calibrating a pH sensor either a Remove the nitrate sensor from the cable bulkhead and plug the port After pH calibration is
38. pH sensor is installed in port 1 or port 2 If the pH sensor is installed in port 1 enable pH in ISE1 of the Sensor Setup menu If the pH sensor is installed in port 2 enable pH in ISE2 of the Sensor Setup menu Note It is not recommended to use a pH ORP combo sensor in 6051010 or Quatro cables If using a pH ORP combo sensor in a 6051010 or Quatro cable ORP will not be measured or reported 3 If using a 6051010 or Quatro cable you must have a sensor installed in port 1 for port 2 to operate Additionally ensure that the sensor installed in port 1 is in good working order In 6051010 and Quatro cables the sensors installed in port 1 and port 2 use the reference from the sensor installed in port 1 only Therefore if the sensor installed in port 1 is not working properly the readings from the sensor installed in port 2 will be erroneous For greatest ease install a pH sensor in port 1 of both 6051010 and Quatro cables and your other ISE sensor in port 2 4 Ifyou continue to get error messages during calibration clean and recondition the sensor Cleaning and Reconditioning the pH ORP or pH ORP Sensor If the pH or pH ORP sensor has been allowed to dry out or has been stored in distilled or deionized water for an extended period of time soak the sensor in buffer 4 overnight to try and restore functionality Cleaning is required whenever deposits or contaminants appear on the glass and or platinum surfaces or when the sensor s response slows
39. roceed with its calibration with no stabilization delay Or d Calibrate pH first immersing both sensors in the pH buffers After calibrating pH place the sensors in 1 000 mg L chloride standard and monitor the reading Usually the reading starts low and may take awhile to reach a stable value When it does proceed with the calibration This may take several hours The chloride sensor can be calibrated with up to three calibration points For highest accuracy perform a two point calibration with 10 and 1000 mg L standards within 10 C of your sample temperature Rinse the sensors and cal cup with a small amount of chloride solution 10 mg L for the first point and 1 000 mg L for the second point Fill the cup so that the chloride sensor tip and the temperature sensor are submerged in solution If using a Quatro cable or 6051030 cable ensure that the conductivity sensor is also submerged in the calibration solution The salinity reading from the conductivity sensor is used in the algorithm for the chloride measurement After entering the calibration screen change the calibration value if necessary If not using a conductivity sensor enter the salinity value of the calibration standard After calibration change the salinity correction value to the salinity value of the water you will be testing in the field in order to obtain the most accurate chloride measurement You can change the salinity correction value in the Sensor menu As mentioned t
40. s should be 90 to 130 mV The slope should be 45 to 65 mV per decade Wait for the ammonium and temperature readings to stabilize in each calibration solution and then press enter to accept each calibration point Rinse the sensor and cal cup between calibration points with a small amount of the next buffer After pressing enter to accept your last calibration point press cal to complete the calibration Otherwise you will continue calibrating up to 3 calibration points 15 12 If you receive a warning message stating that the calibration is guestionable do not continue with the calibration Instead select No and investigate what is causing the questionable results If you accept a questionable calibration your ammonium and ammonia readings will be erroneous Typical causes for this error message include incorrect Sensor Port setup in the instrument a dirty sensor or bad buffer solution Preparing Ammonium Calibration solution We recommend using YSI calibration solutions whenever possible However qualified users can save cost by following these recipes for 1 and 100 mg L standards Other concentrations can be made by altering the amount of ammonium chloride All other ingredient concentrations should remain unchanged It is important to note that some of these chemicals are hazardous and therefore the standards should only be prepared by qualified chemists in laboratories where proper safety precautions are possible It is the re
41. so that the pH sensor tip and the temperature sensor are submerged in buffer 7 If necessary highlight the Calibration Value and enter the pH value of the buffer solution Note The Pro Plus has auto buffer recognition which can be set to USA 4 7 10 or NIST 4 01 6 86 9 18 buffer values in the pH Sensor Setup menu 8 Record the pH millivolts for each calibration point The acceptable mV outputs for each buffer are shown below pH 7 mV value 0 mV 50 mV pH 4 mV value 165 to 180 from 7 buffer mV value pH 10 mV value 165 to 180 from 7 buffer mV value e A value of 50 or 50 mVs in buffer 7 does not indicate a bad sensor e The mV span between pH 4 and 7 and 7 and 10 mV values should be 165 to 180 mV 177 is the ideal distance The slope can be 55 to 60 mV per pH unit with an ideal of 59 mV per pH unit e f the mV span between pH 4 and 7 or 7 and 10 drops below 160 clean the sensor and try to recalibrate 9 Wait for the pH to stabilize in the each buffer and then press enter to accept each calibration point 10 Rinse the sensor and cal cup with a small amount of the next buffer between calibration points 11 After pressing enter to accept your last calibration point press cal to complete the calibration Otherwise you will continue calibrating up to 6 calibration points 12 If you receive a warning message stating that the calibration is guestionable do not continue with the calibration Instead select No
42. sor is still not calibrating after performing a sensor cleaning contact your local YSI Representative or a YSI Authorized Service Center ORP The ORP calibration should be verified every day the instrument is used However a new ORP sensor may be capable of holding its calibration for several days CALIBRATION TIPS 1 2 If using a pH ORP combination sensor calibrate pH first to ensure it is working If using an ORP sensor in a 6051010 or Quatro cable calibrate the sensor in port 1 prior to calibrating the sensor in port 2 The sensor in port 2 uses the reference of the sensor installed in port 1 Therefore it is important to verify that the port 1 sensor is working properly before calibrating the port 2 sensor See ORP Troubleshooting Tips for additional info Rinse the sensors and cal cup with a small amount of ORP calibration solution Fill the cup so that the ORP sensor tip and the temperature sensor are submerged in solution Enter the calibration value per the temperature reading The value of ORP calibration solution is greatly affected by temperature The ORP solution should include a chart of solution values per temperature If not contact the supplier of the ORP solution to obtain this information The Pro Series ORP sensors use a Ag AgCl 3 5 M KCI reference Be sure the value you enter is for this type of reference If using the YSI Zobell calibration solution the Pro Plus will automatically determine the calibration value
43. sponsibility of the user to obtain and study the MSDS for each chemical and to follow the required instructions with regard to handling and disposal of these materials You will need solid Ammonium Chloride or a certified 100 mg L NH4 N from a supplier Lithium Acetate Dihydrate concentrated hydrochloric acid high purity water a good quality analytical balance a 1000 ml volumetric flask accurate volumetric measuring devices for 100 ml and 10 ml of solution and a 1000 ml glass or plastic storage vessels Caution Hydrochloric acid is highly corrosive and toxic and should therefore be handled with extreme care in a well ventilated fume hood The user could also add the equivalent amount of a less hazardous more dilute sample of the acid if preferred 100 mg L Standard Accurately weigh 0 3817 g of ammonium chloride and transfer quantitatively into a 1000 ml volumetric flask Add 2 6 g of lithium acetate dihydrate to the flask Add approximately 500 ml of distilled or deionized water to the flask swirl to dissolve all of the reagents and then dilute to the volumetric mark with distilled or deionized water Mix well by repeated inversion and then transfer the 100 mg L standard to a storage bottle Add 3 drops of concentrated hydrochloric acid to the bottle then seal and agitate to assure homogeneity Alternatively 100 ml of certified 100 mg L NH N standard can be used in place of the solid ammonium chloride 1 mg L Standard Accurately measure
44. sted under Cleaning a Sensor Port 1 Align the connectors of the sensor and the port With connectors aligned push the sensor in towards the bulkhead until you feel the sensor seat in its port You will experience some resistance as you push the sensor inward this is normal 2 Once you feel the sensor seat into the port gently rotate the stainless steel sensor nut clockwise with your fingers do not use the tool 3 The nut must be screwed in by hand If the nut is difficult to turn STOP as this may indicate cross threading If you feel resistance or cross threading at any point unscrew the nut and try again until you are able to screw the nut down completely without feeling any resistance Damage to your cable sensor may occur if you force the parts together 4 Once completely installed the nut will seat flat against the bulkhead At this point use the installation tool that was included with the sensor to turn the nut an additional to Y turn Do not over tighten 5 Please refer to the sensor installation sheet that is included with the conductivity temperature sensor for detailed instructions 25 Cleaning a Sensor Port If you suspect port contamination you can clean the port on the cable by filling the port with Isopropyl Alcohol for 30 seconds and then dumping it out Next allow the port to air dry completely or blow it out with compressed air Installing a sensor into a port that is not completely dry is likely to cause erra
45. ter wipe with a cotton swab moistened with clean water not DI water and then re rinse with clean water To be certain that all traces of the acid are removed from the sensor crevices soak the sensor in clean tap water for about an hour with occasional stirring If biological contamination of the reference junction is suspected or if good response is not restored by the above procedures perform the following additional cleaning step CAUTION Do not mix the acid from the previous step with the chlorine bleach in the following step A toxic gaseous product can form from the reaction between the acid and the chlorine bleach Be certain to copiously rinse the sink and drain system of acid after its disposal and before the disposal of chlorine bleach 1 Soak the sensor for approximately 1 hour in a 1 1 dilution of commercially available chlorine bleach 2 Rinse the sensor with clean water and then soak for at least 1 hour in clean tap water with occasional stirring to remove residual bleach from the junction If possible soak the sensor for a period of time longer than 1 hour in order to be certain that all traces of chlorine bleach are removed Then re rinse the sensor with clean water and retest Prior to reinstalling the sensor dry the port and sensor connector with compressed air If you suspect port contamination follow the instructions in the Cleaning a Sensor Port section of this document before reinstalling the sensor If your pH sen
46. the nitrate sensor is installed in port 2 enable nitrate in ISE2 of the Sensor Setup menu Note If using with a pH or ORP sensor it is recommended to install the pH or ORP sensor in port 1 and the nitrate sensor in port 2 3 If using a 6051010 or Quatro cable you must have a sensor installed in port 1 for port 2 to operate Additionally ensure that the sensor installed in port 1 is in good working order In 6051010 and Quatro cables the sensors installed in port 1 and port 2 use the reference from the sensor installed in port 1 only Therefore if the sensor installed in port 1 is not working properly the readings from the sensor installed in port 2 will be erroneous as well 19 4 If you continue to get error messages during calibration clean the sensor 5 If you continue to get error messages during calibration soak the sensor in 100 mg L nitrate standard for several hours or overnight 6 If you suspect port contamination follow the instructions in the Cleaning a Sensor Port section 7 If you continue to have trouble calibrating the nitrate sensor contact your local YSI Representative or a YSI Authorized Service Center Cleaning and Reconditioning the Nitrate Sensor The nitrate sensor uses a PVC membrane As always when handling a sensor care should be taken to avoid damaging the membrane After extensive use the membranes may become coated with a deposit or scoured with fine scratches which may cause a slow or reduced response
47. tic and erroneous readings If the connector is corroded contact your local YSI Representative or a YSI Authorized Service Center Verifying Sensor Accuracy and Calibration Sensor accuracy and calibration can be verified by immersing a sensor into calibration solution or YSI Confidence Solution Compare the readings on the Pro Plus display to the value of the solution If the readings have drifted more than the accuracy specification of the sensor perform a calibration before taking field measurements YSI Confidence Solution can be used to check the accuracy and calibration of the conductivity pH and ORP sensors However to maintain the highest accuracy of the instrument it should not be used to perform a calibration Resetting a Sensor to Factory Default Occasionally it may be necessary to reset the instrument to its factory calibration default values To reset the calibration values press the Cal key highlight Restore Default Cal and press enter Highlight the parameter you wish to reset to default and press enter Next you will be asked to confirm the operation Highlight Yes and press enter to confirm Professional Plus Calibration Tips Rev A December 2010 26
48. tion with an ammonium sensor during measurements If a pH sensor is not in use the instrument will assume the sample is neutral pH 7 for the calculation CALIBRATION TIPS 1 10 11 If using an ammonium sensor with either a pH or ORP sensor on a 6051010 or Quatro cable install the pH or ORP sensor in port 1 and the ammonium sensor in port 2 If using an ammonium sensor on a 6051010 or Quatro cable calibrate the sensor in port 1 prior to calibrating the sensor in port 2 The sensor in port 2 uses the reference of the sensor installed in port 1 Therefore it is important to verify that the sensor in port 1 is working properly before calibrating the sensor in port 2 See ammonium Troubleshooting Tips for additional information on port configuration Exposure to the high ionic content of pH buffers can cause a significant but temporary drift in the ammonium sensor Therefore if calibrating a pH sensor either a Remove the ammonium sensor from the cable bulkhead and plug the port After pH calibration is complete reinstall the ammonium sensor and proceed with its calibration with no stabilization delay Or b Calibrate pH first immersing both sensors in the pH buffers After calibrating pH place the sensors in 100 mg L ammonium standard and monitor the reading Usually the reading starts low and may take awhile to reach a stable value When it does proceed with the calibration This may take several hours The ammonium sensor can
49. uses the instrument s barometric pressure reading for the DO calibration If the barometer is not reading accurately the calibration will be erroneous The barometer should be reading true barometric pressure If you suspect the barometer reading is incorrect calibrate the barometer and then recalibrate the DO sensor Laboratory barometer readings are usually true uncorrected values of air pressure and can be used as is for barometer calibration Weather service readings are usually not true i e they are corrected to sea level and therefore cannot be used until they are uncorrected An approximate formula for this uncorrection is True BP in mmHg Corrected BP in mmHg 2 5 Local Altitude in ft above sea level 100 Install a new membrane with fresh electrolyte onto the DO sensor Ensure you are using the correct electrolyte solution Polarographic sensors use electrolyte that is in a white labeled bottle KCI Na2SO4 Galvanic sensors use electrolyte that is in a blue labeled bottle NaCl Recondition the DO sensor and then install a new membrane If you suspect port contamination remove the sensor and follow the instructions in the Cleaning a Sensor Port section If you continue to have trouble calibrating the DO sensor contact your local YSI Representative or a YSI Authorized Service Center Membrane Cap Installation The DO membrane and electrolyte solution O2 solution should be changed once every
50. will turn dark in color This discoloration is normal however it is recommended that you remove the deposits as needed Perform the following cleaning procedures to remove the deposits if 1 You have troubles calibrating the sensor or the DO readings are unstable and 2 Changing a membrane does not correct the problem Silver Anode After extended use a layer of Silver Chloride AgCI builds up on the silver anode reducing the sensitivity of the sensor The anode must be cleaned to remove this layer and restore proper performance The cleaning can be chemical and or mechanical Chemical cleaning Remove the membrane cap and rinse the electrodes with deionized or distilled water Soak the sensing electrode section of the sensor in a 14 ammonium hydroxide solution for 2 to 3 minutes or in a 3 ammonia solution overnight for 8 12 hours most household ammonia cleaners are typically around 3 Rinse heavily in cool tap water followed by a thorough rinsing with distilled or deionized water The anode should then be thoroughly wiped with a wet paper towel to remove the residual layer from the anode Trapping residual ammonia under the new membrane cap can quickly tarnish the electrode and or give false readings Note Chemical cleaning should be performed as infrequently as possible 1 or 2 times per year depending on use First attempt a membrane change and recalibrate If a new membrane does not resolve the problem then proceed with cleaning A
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