SenSorTrace BaSIc 3.0 uSer manual
Contents
1. cco ene TITT TT SESS SSP SSS SSS SSS SS SS PSSST TSS SS SS PSS SSS SSS SSS SS TS SSS SR SSS SS SS PS SSS SS SS SSS SSeS Ss SSS sSSSs as ssessesesss 9999 SSs esses A 4 4 4 4 4 4 4 See See eens han Sk ewes TETTI 1 5 1 155 50 pmol 2 Sensor 2 No calibration Clear SAMPLING INTERVAL The sampling interval denotes how often the program will make a data point for the log file and in the graph A single data poin2. save to a specified file Delete the data file Make new data file New file Comments Pressing COMMENTS will make a dialog appear where a comment can be entered along with a timestamp from the time the button is pushed The timestamp can be updated to the current time by pressing UPDATE TIME This feature allows the user to either 1 press the button just when observing an unforeseen event and then write the appropriate text later or 2 write the text first wait for an expected event and press Update time when the event occurs The events will be shown in the table to the right and an indication will be displayed in the graph in the Strip chart window The comment will be added to the table on the Comments tab 19 OUTPUT FILE All logged data and text entered and stored in your SensorTrace BASIC program are accessible in an Microsoft Excel output file to facilitate processing and graphic representation of the data Importing and converting text files is thus not necessary when using Unisense software However make sure that you have a program that can view or import Microsoft Excel files Microsoft Excel D rerio embryos xls rile Edit View Insert Format Tools Data Window Help Adobe PDF Z 100 2 16 Arial B D Time since start h Sensor 1 pmol l Sensor 2 Sensori mV Sensor 2 mV 2 14 04
3. 5 5 SENSORTRACE BASIC 3 0 USER MANUAL 5 BASIC 3 0 User MANUAL SENSORTRACE BASIC 3 0 USER MANUAL Copyright 2010 Unisense A S Version October 2010 UNISENSE A S TABLE CONTENTS CONGRATULATIONS WITH YOUR NEW PRODUCT SUPPORT ORDERING AND CONTACT INFORMATION OVERVIEW INSTALLING THE SOFTWARE GETTING STARTED SENSORTRACE BASIC INTERFACE SETMNGS wees COMMENTS CALIBRATIONFIAB CALIBRATION PROCEDURE LOGGER TAD SAMPLING INTERVAL RECORDING DATA WARRANTY AND LIABILITY REPAIR OR ADJUSTMENT CONGRATULATIONS WITH YOUR NEW PRODUCT SUPPORT ORDERING AND CONTACT INFORMATION If you wish to order additional products or if you encounter any problems and need scientific technical assistance please do not hesitate to contact our sales and support team We will respond to your inquiry within one working day E mail sales unisense com Unisense A S Tueager 1 DK 8200 Aarhus N Denmark Tel 45 8944 9500 Fax 45 8944 9549 Further documentation and support is available at our website www unisense com OVERVIEW SensorTrace BASIC is
4. 83 630 863 14 04 2009 13 36 22 0 561127 172 837 0 446 391 630 923 28 14 04 2009 13 36 23 0 561408 172 564 0 445 687 630 981 29 14 04 2009 13 36 24 0 56169 172 518 0 445 567 631 003 30 14 04 2009 13 36 26 0 561972 172 317 0 445 049 631 039 31 14 04 2009 13 36 27 0 562254 172 199 0 444 743 631 068 32 14 04 2009 13 36 28 0 562535 171 917 0 444 013 631 114 33 14 04 2009 13 36 29 0 562817 171 823 0 443 77 631 172 34 14 04 2009 13 36 30 0 563099 171 682 0 443 404 631 302 35 14 04 2009 13 36 31 0 563381 171 572 0 443 121 631 4 36 14042009 13 36 32 0 563663 171 467 0 442 849 631 444 37 14 04 2009 13 36 33 0 563945 171 535 0 443 026 631 467 38 14 04 2009 13 36 34 0 564227 171 234 0 442 247 631 516 39 14 04 2009 13 36 35 0 564508 171 313 0 442 452 631 53 40 14 04 2009 13 36 36 0 56479 171 155 0 442 043 631 605 41 14042009 13 36 37 0 565072 170 911 0 441 412 631 671 14 04 2009 13 36 38 0 565354 171 026 0 441 71 631 728 43 14 04 2009 13 36 39 0 565636 170 966 0 441 555 631 766 Comments Sensors Calibration Calibration Loggerf 0000077 Ready 20 The worksheets in the Excel file reflect the tabs in the SensorTrace BASIC software However for technical reasons there are two sheets of calibration data see picture and the table below SensorTrace BASIC tabs Excel worksheet Settings Sensors Comments Comments Calibration Calibration data and Calibration Logger
5. Logger DIRECT EXPORT OF GRAPHS The content of all graphs in SensorTrace BASIC can be exported either as graphics or as a data file This option is particularly useful for conserving the data in the Strip Chart which is not saved in the normal Excel file To export a graph right click on the graph This returns a small menu with two options Export and Print Choosing Export makes a dialog box appear with three tabs Picture Native and Data and the buttons Copy SAVE SEND and CLOSE as Metafile The chosen tab determines the type of data e Picture the data is a graphic either a bitmap WARRANTY AND LIABILITY or a Windows metafile as determined on the tab Microsensors are consumable items The sensors are tested e Native this tab is redundant and can be thoroughly before packaging and shipment disregarded e Data the data are data values a format Warranty for sensors chosen by the user on the tab It is possible sensors 60 days from shipment Standard Oxygen sensors 180 days from shipment to choose only one sensor in the series yg y p All other sensors excluding special sensors 90 days from shipment drop down box Each sensor is mentioned 21 D l l If within the above specified period the sensor s fail to perform two times First time a sensor is mentioned Gh cog according to the specifications Unisense
6. in the graph in the Strip chart window In addition to the user generated comments the program will time stamp and show events for instance calibrations All comments and events are stored in the output file 13 CALIBRATION TAB Calibrations are performed in the Calibration tab The buttons in the top left corner show whether the sensors are calibrated or not the layout will change if there are more than eight sensors Uncalibrated Calibrated 1 Sensor1 699 20 mV 2 Sensor 2 604 30 mV Choose the sensor you would like to calibrate The sensor name type and calibration unit is shown under Active calibration Signal Shows the current raw sensor signal for the chosen sensor The sensor signal can also be followed continuously in the strip chart window in the bottom of the tab CALIBRATION PROCEDURE Prepare the calibration samples Choose the sensor you want to calibate Change the concentration in the concentration box according to the actual calibration solution For oxygen an automated procedure to calculate the atmospheric saturation as a function of temperature and salinity can be invoked by pressing the button named O2 After entering the correct concentration add the calibration point by pressing ADD POINT Several points can be added for each concentration Change to another calibration standard and repeat points 3 4 It is possible to use several d
7. should not be chosen so small that the signal gets beyond the selected range This will The Settings tab shown with all menus down for overview cause the A D converter to get saturated Name Write a name describing your sensor optional Comment Write a comment about your sensor optional BACKGROUND FREQUENCY To the right of the table SensorTrace BASIC allows you to select the frequency of the background data acquisition This determines the frequency with which the A D converter reads the signal from the instruments If you are using a computer which be slow or less powerful than specified in the computer requirements for the program you can set this to slow This will cause the A D converter to read data with a speed of 100Hz 100 times per second instead of 1000 Hz This will put less strain on the computer but result in more noisy measurements In most instances especially if your computer meets the standards in the specifications you should leave this on normal The background frequency is NOT the same as the sampling interval chosen in the Logger tab Each logged point is an average of many measurements The sampling interval denotes how often a data point is logged The background frequency determines how many measurements are included in the average read more in the section on the Logger tab under Sampling interval Note that settings cannot be changed after pressing Start When the settings are appro
8. will replace the sensor s data represents uncalibrated data the free of charge second time it represents calibrated data Unisense will only replace dysfunctional sensors if they have The buttons have the following functions been tested according with the instructions in the manual copies data to the Windows Clipboard upon receipt of the sensor s The warranty does not include and can then be pasted directly into Excel or repair or replacement necessitated by accident neglect misuse unauthorized repair or modification of the product other program by pressing CTRL V or Edit Physical damage to the tip of the sensor is not covered by the Paste warranty SAVE prompts for a filename and path where the data is saved Liability In no event will Unisense be liable for any direct indirect SEND sends data as an attachment to an consequential or incidental damages including lost profits or for email any claim by any third party arising out of the use the results of e CLOSE closes the dialog use or the inability to use this product REPAIR OR ADJUSTMENT Sensors and electrodes cannot be repaired Other equipment that is not covered by the warranty will if possible be repaired by Unisense with appropriate charges paid by the customer In case of return of equipment please contact us for a return authorization For further information please see the documents Conditions for Sale and Deli
9. 2009 13 35 57 0 554091 175 207 0 452 521 629 612 14 04 2009 13 35 58 0 55437 175 033 0 452 07 629 656 4 14042009 13 35 59 0 554648 174 784 0 451 427 629 704 5 14 04 2009 13 36 00 0 55493 174 955 0 451 869 629 731 6 14 04 2009 13 36 01 0 555212 174 8 0 451 469 629 799 14 04 2009 13 36 02 0 55549 174 44 0 450 537 629 887 8 14 04 2009 13 36 03 0 555771 174 46 0 450 589 629 926 9 14 04 2009 13 36 04 0 556053 174 478 0 450 634 629 979 10 14 04 2009 13 36 05 0 556335 174 318 0 450 222 630 027 11 14 04 2009 13 36 06 0 556617 174 031 0 449 479 630 072 12 14 04 2009 13 36 07 0 556898 173 969 0 449 318 630 139 13 14 04 2009 13 36 08 0 557181 173 795 0 448 868 630 204 44 14042009 13 36 09 0 557462 173 537 0 448 202 630 266 15 14 04 2009 13 36 10 0 557744 173 711 0 448 653 630 316 16 14 04 2009 13 36 11 0 558026 173 464 0 448 015 630 401 47 14 04 2009 13 36 12 0 558308 173 463 0 448 01 630 448 18 14 04 2009 13 36 13 0 558589 173 406 0 447 863 630 474 19 14 04 2009 13 36 14 0 558871 173 427 0 447 918 630 527 20 14 04 2009 13 36 15 0 559154 173 308 0 447 609 630 592 21 14 04 2009 13 36 16 0 559435 173 256 0 447 477 630 673 14 04 2009 13 36 17 0 559717 173 16 0 447 227 630 722 23 14 04 2009 13 36 18 0 559999 173 25 0 447 46 630 759 14 04 2009 13 36 19 0 560281 173 004 0 446 824 630 804 25 14 04 2009 13 36 20 0 560562 172 749 0 446 165 630 847 26 14 04 2009 13 36 21 0 560844 173 006 0 446
10. cale By default the y axis autoscales to accommodate the maximum and minimum signals that are shown in the strip chart The y axis scale can be modified on the Chart format dialog see below which appears when right clicking on the y axis NB only works after pressing Start If minimum and maximum values are entered into the Value fields in this window un zooming will return the scale to these values Calibrated un calibrated The user can control whether the graphs show calibrated signals or raw signals for the sensors by checking the Calibrated checkbox for each graph If no calibration has been performed see Calibration tab section below and the checkbox is checked no signals will be plotted Chart legend To the right of the chart there is a legend showing the graph format for the associated sensors and their current signal concentration value Datapoints The datapoints in the Strip chart window can be cleared by pressing CLEAR This will NOT affect the stored data measured in the Logger tab THE CHART FORMAT DIALOG The Chart format dialog appears when the Y axis of the Strip chart window is pressed only works after Start has been pressed It is possible to use multiple y axes up to four in each chart in SensorTrace BASIC This allows the user to view sensor signals values of different scales in the same graph Modifications of axes and signal representation can be made for both calibrated and u
11. ecked calibrated The lower area the Strip Chart shows the sensor signals and event values are plotted for calibrated sensors The strip chart holds a marks continuously to provide the user an overview of the entire maximum of 24 hours of measurements experiment The strip chart is shown in all tabs of the program You can change the height of the window by dragging its upper border The upper area is divided into tabs which allow the user to access different functions of the program All components will be described in detail on the following pages 1 Sensor 1 2485 00 mV 2 Sensor 2 852 10 mV Comments calibrations points and other events generated by the user or the program can be seen as colored marks in the strip chart window By holding the cursor over the mark the associated text is displayed next to the mark See also the section on the Comments tab for further information on comments and events X axis scale By default the time scale x axis is controlled in the Show last min box where a number of preset time intervals can be chosen To have a look at a certain time span zoom in on this area by dragging a rectangle with the mouse from the upper left corner to the lower right corner of the area of interest only works if the Y zoom only box to the right is un checked To un zoom drag a rectangle from the lower right to the upper left corner Y axis s
12. ifferent standards and make multi point calibration to verify linearity If any calibration points are not valid e g due to typing errors a single point can be cleared by right clicking the point and choosing Delete point All calibration points can be removed by pressing CLEAR ALL POINTS When you are satisfied with your calibration press APPLY CALIBRATION A linear regression will be performed based on the calibration and this regression will form the basis for converting signals to calibrated values Values are displayed in the table Repeat 2 7 for other sensors The calibration table below the calibration graph shows the 15 calibrations for the chosen sensor Each calibration will appear here with information on calibration number time of calibration linear regression data slope intercept and as well as additional user comments that can be entered directly into the table For information on calibration of specific sensors consult the sensor manuals RECALIBRATION PROCEDURE Sensors can be recalibrated at any time during an experiment The new calibration applies from the time of calibration onward To recalibrate the sensor 1 Press CLEAR ALL POINTS 2 Follow step 1 6 in the Calibration procedure All calibration data are stored in the output excel file RETRIEVING A CALIBRATION Pressing RETRIEVE CALIBRATION will retrieve the calibration of the sensor made the last time the program was ru
13. ncalibrated signals on separate tabs The Chart Format dialog when two In the upper part of the Chart Format window you can modify the maximum minimum values each axis default the axes are set to autoscale to accommodate the maximum and minimum concentrations signals shown in the chart but you can modify the maximum and minimum values by typing new values in the appropriate boxes In the lower part of the Chart Format window you can assign sensors to the different axes by choosing the desired Axis number for each sensor In this area you can also set the visibility and format of lines and marks on the graphs SETTINGS TAB After the filename has been selected in the startup dialog the first tab to appear in the upper area is the Setttings tab This tab will display the detected hardware and sensors In the Sensors table various parameters for the sensor s can be chosen 1 Sensor 1 2485 00 2 Sensor 2 852 10 mV Sensor Mark the checkboxes for the sensors you want to view and record signals from Type Choose sensor type from the drop down menu if the default value is not appropriate Unit Select an appropriate concentration unit for the sensor signal when calibrated Range V Select the voltage range for the AD converter Select this range as small as possible to get the most out of the resolution of the A D converter However the range
14. nning If you want to use the retrieved calibration remember to press APPLY CALIBRATION after retrieving a calibration Note When retrieving a calibration you will load the last calibration made the previous time the program was running Make sure that the retrieved calibration matches your current sensor in terms of signal size and units IMPORTANT When retrieving a calibration you will load the last calibration made the previous time the program was running Make sure that the retrieved calibration matches your current sensor terms of signal size and units LOGGER TAB The Logger tab is where data collection is performed SensorTrace Basic 2 0 D rerio embryos xls Jawslina intal G 10 aenn Per ee ey Te Ee TT LCP Te TT Ee Te ee 1 Sensor 1 2 Sensor 2 etctoclucaocstaccsescdecdccsecuceesedscccsecesecnelecccesec eases ee eer eee Gee eee E dew wwe T
15. priate press START The sensor and A D converter settings will be saved and two more tabs will appear Calibration and Logger 11 COMMENTS TAB The Comments tab allows the user to enter notes and comments regarding the measurements in the file 1 Sensor 1 2755 00 mV 2 Sensor 2 916 30 File shows the path and the filename selected previously in the Start dialog Experiment notes In this field any text that you want to save with your data e g a general description of your experiment can be entered Comments SensorTrace BASIC allows the user to make comments with a timestamp which will be displayed on both the Strip Chart and Logger graphs This enables the user to get a better overview of the course of an experiment Pressing COMMENT keyboard shortcut Ctrl K will make the following dialog appear where TIP The keyboard shortcut for adding acomment is Ctrl K the comment can be entered along with a timestamp from the time the button is pushed The timestamp can be updated to the current time pushing the TIME button Thus this feature allows the user to either 1 press the button just when observing an unforeseen event and then write the appropriate text later or 2 write the text first wait for an expected event and press UPDATE TIME when the event occurs The events will be listed in the table to the right and an indication will be displayed
16. t consists of an average of measurements sampled with the background frequency in the sampling interval preceeding the data point up to 10 s In other words with a sampling interval of 10s the data points are logged every 10s as an average over 1000 samples per second during the previous 10s The Background frequency is preset to 1000Hz and can be changed on the Settings tab Background frequency It is important to know the system you are measuring in in order to choose the right sampling interval and not average out the fluctuations that you are expecting For a relatively active system 17 for example it is preferable to choose a short sampling interval For along perhaps linear monitoring situation a longer sampling interval will save computer memory The sampling interval cannot be changed while logging If you need to change your sampling interval sampling must be interrupted PAUSE or SAVE see below before the interval can be changed Resume logging by pressing In the upper right corner you can follow the Memory status on the amount of free memory space and on the number of points made RECORDING DATA Before you start the recording choose the relevant sampling interval Then press REC and the data will be logged in the PC memory An Excel file is created when you close the program or when you press SAVE O Record data Rec Pause data J Stop data acquisition
17. the basic data acquisition program from the Unisense program package It offers timeseries datalogging and calibration features SensorTrace BASIC is compatible with all digital Unisense instruments It supports up to 16 channels with an adjustable logging frequency of up to 10 samples per second The program automatically saves all data in an MS Excel formatted file for subsequent data analysis The software is freeware and can be downloaded from our webpage System requirements e A digital Unisense amplifier or A D converter unit e PC with e Windows XP 2000 Vista e 100 MB free hard disk space e USB port MS Excel or other Excel file viewer Other programs For motor control activity calculations and profiling in 1 2 or 3 dimensions we recommend that you use SensorTrace PRO For photosynthetic experiments using the light dark switch technique we recommend that you use SensorTrace PHOTO INSTALLING THE SOFTWARE Make sure that you are installing in the administrator mode Start the installation program exe file from the CD Follow the instructions given by the installation program This will install the SensorTrace BASIC program and a version of this manual in the program group Unisense A configuration utility for the A D converter will also be installed This is called Instacal and will be placed in a program group called Measurement Computing GETTING STARTED 1 Make sure that yo
18. ur PC does not enter sleep mode or stand by during measurements as this will interrupt the connection to the instruments and it will be necessary to restart the program Connect all instruments in your set up to the computer Start SensorTrace BASIC it is placed in the program group Unisense The following dialog appears 5 Basic 3 0 2 SensorTrac SensorTrace Basic v3 0 2 New experiment Load old experiment Choose either to make a new experiment or load an old experiment a New experiment A dialog appears were you can choose name and location of your new data Load old experiment A dialog appears were you can choose the file you would like to open This mode is for UNISENSE IMPORTANT Please make sure that your PC does not enter sleep mode or stand by during measurements as this will interrupt the connection to the instruments and it will be necessary to restart the program working with old data settings parameters cannot be changed and THE STRIP CHART new measurements cannot be started 5 Press OK The Strip Chart graph is permanently visible in the lower part of the SensorTrace BASIC interface It allows the user to view sensor SENSORTRACE BASIC INTERFACE signals continuously By default uncalibrated raw sensor signals The SensorTrace BASIC interface is divided into two main areas are shown but if the Calibrated check box is ch
19. very for and Warranty and Shipping Information 22 23 TROUBLE SHOOTING Problem Possible cause 1 Possible cause 2 Possible cause 3 Solution Problem Possible cause 1 Solution Possible cause 2 Solution Solution 24 Following error message appears x A D converter is not recognised Board S N 0 If problem persist please contact Unisense Do you want to start configuration utility Yes No You have not connected your digital sensor instrument or A D converter The program does not recognize the analog to digital converter An A D converter with different bit resolution has just been connected Choose yes to open configuration utility InstaCal InstaCal will clear any old A D converters not connected to your system and search for connected A D converters Noisy measurements Physical vibrations from other appliances on the table are causing movements of the sensor resulting in instability of experimental set up and disturbances in the measurements Remove all unrelated appliances from surfaces in contact with the experimental set up and ensure completely stable conditions for the sample Electric noise in the system Check that the system is properly grounded Connect the Ground connection on your sensor instrument to a ground source a waterpipe or similar Sometimes it can also help to ground the meter directly to your measuring set up
20. with a wire going from the Ground connection of the sensor instrument to the liquid you are measuring in Change the Background frequency 5 5 UNISENSE DENMARK www unisense com info unisense com
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