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getSoft for getSpec–102/256/1024/2048 version 7.0 USER`S MANUAL

1. D3 Value for first History Channel 0 if spectrum is selected D5 D Spectrum for first History Channel E5 E Wavelength scale in nm for second History Channel spectrum empty if spectrum is not selected F3 Value for second History Channel F5 F Spectrum for second History Channel empty if soectrum is not selected depends on the wavelength range selected 4 5 4 Start Output You start the output the usual way with Application gt History gt Start measuring You can also use the corresponding button on the button bar Please do not perform large alterations of the worksheet sw5 noi E xi while data is being transmitted An error Call was rejected by callee will be issued if Excel is too busy e Call was rejected by callee Moving around the worksheet should not pose any problems Save any alterations to your worksheet before starting the transfer to Excel You do not have to close the worksheet or close Excel 4 5 5 Stop Output If you want to stop the transfer to Excel give focus to getSoft and press Exit It may take a few seconds before the buttons appear E oHistory Channel Functions Exit Pause m Info Fe User Defined Function 1 800 Scope Made Output 10 24 Excel will not be closed by getSoft Save your work and close Excel the same way you would when working with Excel manually We have included a sample worksheet called Continuous xls whi
2. 3 2 9 5 Setup Menu Options Correct for Drift USB1 platform only Correct Far Drift bv FOS 2 INLIME LIVIvIS Automatic Save Dark by TTL shutter by Spectrometer Channel Introduction When measuring the reflectance of a white reference tile or the transmittance of a reference solution against time the output should theoretically remain 100 noise In practice the output value will not remain exactly fluctuating round 100 but the signal can slowly drift away The cause for this drift in the measurement system can be a change in temperature in the optical bench which causes micro bending of the components that focus the light at the detector but also a drift in the light source that is used to illuminate the reference sample To correct for the drift in the system it needs to be measured first This can be done with a 2 channel fiber optic switch FOS 2 INLINE UV VIS in which case one channel is connected to the reference sample and the other channel to the sample that needs to be measured By regularly switching channels manually or automatically the measured deviation from 100 96 at the reference channel can be used to compensate the measured data at the sample channel The same principle can be used if a 2 or multiple channel spectrometer is available In that case the sample and reference data can be measured simultaneously and the measured data at the reference sample can be used directly to correct the sample data Disa
3. c BRI Dateiname Test kon I Dateityp Esperiment Files Abbrechen E lfnen With this option an experiment can be loaded that has been used before This way more spectra can be saved to an existing experiment An experiment name has the file extension kon After choosing this option a dialog box shows all experiments that were saved earlier in the current experiment directory If the experiment name that needs to be loaded is in this directory select it and click the Save button If the experiment name that needs to be loaded is in another drive and or directory move to this directory by clicking the sl behind the current folder name For detailed information on graphic filenames see section 3 1 7 File Menu Save Experiment 3 1 5 File Menu Save Dark With this option dark data are saved For USB1 platform the name of the dark data file is dark dat where represents the number of the slave channel for which the dark data has been saved 0 represents the master channel For USB2 platform the name of the dark data file is serialnr drk The dark data files will be saved in the experiment directory that has been picked by the option File gt Load Experiment or File gt Start gt New Experiment info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 2
4. more than spectroscopy APPENDIX A TROUBLESHOOTING How to rectify an incorrect USB installation info getSpec com Tel Fax 449 0 351 871 8423 449 0 351 871 8465 T l Fax 433 0 4 72 80 82 02 433 0 4 72 80 82 43 Tel Fax 449 0 231 974 2285 449 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 4 more than spectroscopy Gel Spec com 0 getSoft Installation Before you connect the getSpec 2048 spectrometer to the USB port of your computer you have to install the getSoft software GetSoft version 7 is a 32 bit application and can be installed under the following operating systems o Windows 95 98 Me o Windows NT 2000 XP If the operating system is Windows 95 or Windows NT4 0 use a standard RS 232 cable with male and female DB 9 connectors to connect the getSpec 2048 to the serial port of the computer Installation program Every new spectrometer system includes a getSpec com product CD ROM After the CD ROM is inserted in the CD ROM drive the main menu shows the option Install getSoft software After selecting this option a submenu is displayed in which the spectrometer configuration can be selected The getSpec USB1 group of spectrometers should be selected to install getSoft for one of the following spectrometer types getSpec 102 or getSpec 102 y getSpec 256 or getSpec 256 y getSpec 1024 or getSpec 1024 y getSpec 2048 or getSpec 2048 y
5. 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 12 more than spectroscopy Gel spec com ii Save experiment button By clicking the Save Experiment button an experiment is saved The same result can be achieved with the option File gt Save gt Experiment Print button By clicking the Print button a graph that is displayed on the monitor will be printed The same result can be achieved with the option File gt Print El bal OI Channel button USB1 platform only After clicking the Channel button a dialog is shown in which the spectrometer channels can be selected for which data will be acquired and displayed The same result can be achieved with the option View gt Channel S Scope button By clicking the S button the data will be presented in scope mode The same result can be achieved with the option View gt Scope Mode Al Absorbance button By clicking the A button the data will be presented in absorbance mode The same result can be achieved with the option View gt Absorbance Mode T Transmission button By clicking the T button the data will be presented in transmittance mode The same result can be achieved with the option View gt Transmittance Mode I Irradiance button p By clicking the I button the data will be presented in irradiance mode The s
6. What A s the ahscrtance or exincbon is the exanciem coefficient of the compound to be measured C ui the Ten conrenirasn and ig ur apace path length cmi Tahin n gractice thes rebysonship is ony mem at reasonably ipw absorbance Jewels zar less than 2 The wavelength st wiuch the Toky in D absarh anre us ne asused mast of course be wept crasta hice clei 1 Procam Conia To take rosceniranbon measurements voa wil reed a spectrometer with either a cuvette bolder ar a dp grabe and a suitable Exi uius aut pau ce gk Loge L Eherveenn gesta Chem can deplay and save the calculated cenceniratoa n feo ways m T THE cencentrabos can he displayed Wibe m amp sepgarabe Gak wand Ini op ta eh pacion chia E avaishin Yoo can zelect ug tn erti Festory dhmt that can dispar ad erre conceniratan values garet tee This opel cun be Combest with the Excel and Fracess Costral appazanors Instead of browsing through the contents to a specific topic the search TAB can be selected as illustrated in the figure below After typing in a keyword e g concentration and clicking the List Topics button a list of all topics containing this keyword is shown By selecting a topic at the left double click the information is displayed at the right in which the keyword is marked in the text Yeg ec sa TTE Coby Faru merri dis ironia madharce Price Coria Tie dialing sarie x diram after selecting Apis Chemomeary
7. 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 47 more than spectroscopy Gel Spec com Level Triggered When this mode is selected the spectrometer will start to accumulate data take scans at the selected integration time at the rising edge of the TTL pulse and will continue to do so as long as the TTL signal remains high When the signal becomes low the average of the accumulated data except for the last scan will be displayed or saved This mode is specially useful for conveyor belt applications when a product needs to be scanned independent of the transport speed 3 2 9 8 Setup Menu Options Auto configure Integration time After this menu option is clicked getSoft starts searching for an optimal integration time Depending on the maximum counts in the last scan the integration time will be increased decreased automatically until an optimal signal is measured The changing values of the actual integration time during the search can be followed in the integration time field at the edit bar If the auto configure integration time routine needs to be aborted before an optimal integration time has been found click the AC button which is in the down position during the search or reselect the menu option Setup gt Options gt Auto Configure Integration time When the maximum peak is around 14000 counts a dialog
8. Fixed Seconds l hin 135 00 Display Last Minutes A iou Mas 105 0 For Integral User Defined and Peak functions the output can be displayed graphically against time The amount of time that will be displayed at the x axis can be set manually by clicking the Fixed radio button If set to Auto the time axis will be set to 1 minute This option is not available if the View Spectrum Function Type has been selected because the x axis range is in that case in nanometers and determined by the wavelength range as described above The y axis can also be set to Fixed or to Auto The auto option will set the y axis range to the minimum and maximum function values that are in the list of measured data points Function Independent Parameters Display No Graphics To Speed Up Data Processing Save Function Qutpu Do not save Function Output save every ja seconds Change Output File Save Function Output Ta File D test History dat x Cancel Save HCF Load HCF Function Independent Parameters Display no graphics to speed up data processing Below the TAB sheets for function definition an option can be enabled or disabled to speed up data processing by not displaying the graphics during the measurements If an application requires fast data processing e g more than 10 scans per second this option should be enabled If saved to an output file the data can be displayed graphically after the time serie
9. Seis forthe imi me An esmi calibration fle excerpiril col baa hern created m Har folder chem weoch hoda the drta ha ui presented n He dalog Hobe Ihi the ERE a E nan Tyi artdi EE aC cages Vath knew concesabon Do crsale TH OW cabras Gels a Geacnbed beher Afer a calroon has bees musd E wi be loaded atomically the nest tre the sppbradkxm m rtarted Orme 3 cx o abon har been reed it can bee jaar beter en arid ode if needed ExuT asus Cakhrarioa A nsu rara cs babed by cbckeng the Eat Hew Caisruison Cea Dial bursa AD de Gelb Por tpe sek spectrometer channel wil be cleaned info getSpec com Tel 49 0 351 871 8423 T l 433 0 4 72 80 82 02 Tel 449 0 231 974 2285 Fax 449 0 351 871 8465 Fax 433 0 4 72 80 82 43 Fax 449 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 112 m ore than spectroscopy Gel Spec com As described in section 4 1 1 History Application Function Entry the History Channel Functions have been extended with the possibility to define a function with the support of a VB ocript We have included a help file for VBScript for your reference You can reach it from the main menu by selecting Help gt VBScript It lists among other things all available operators and functions o 4 wo o 6 fj g Hide Locate Previous Next Back Forvard Stop Refresh Home Print Contents Index Search Favorites VBScript User s Guide El Lf VBScript Q Users
10. more than spectroscopy get Spec COM getSoft for getSpec 102 256 1024 2048 version 7 0 USER S MANUAL info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 1 more than spectroscopy 0 0 1 0 2 1 2 2 1 2 2 2 3 2 4 2 5 2 6 3 1 3 2 Tel Fax Gell spec com GETSOFT INSTALLATION BIUCTOON ING ANA NON sccegiekcaddvnexeentenaiidiveaneciniadedscsaceeabequtndiniaansetindadicdgeawacgeaahogeeuxaned LAUNCHING the software ccc cece cece eee e eee e eee e eee eee ee nene memes enn nere senes QUICK START MEASURING AND SAVING A SPECTRUM MAIN WINDOW Menu bar Button bar Edit bar Graphical region Status bar Find peaks or valleys by CTRL or SHIFT left mouse button click ssuussussee MENU OPTIONS 3 1 3 1 3 1 3 1 3 1 3 1 3 1 3 1 3 1 3 1 10 3 1 11 3 1 12 3 1 13 3 1 14 OOnNDOOFRWD gt 3 2 1 3 2 2 3 2 3 3 2 4 3 2 5 3 2 6 3 2 7 3 2 8 3 2 9 3 2 9 1 3 2 9 2 3 2 9 3 3 2 9 4 3 2 9 5 3 2 9 6 3 2 9 7 3 2 9 8 3 2 9 9 3 2 9 10 File Menu Setup Menu Setup Menu Setup Menu Setup Menu Setup Menu Setup Menu Setup Menu Setup Menu Setup Menu Setup Menu File Menu Start New Experiment cccccecceceeeeceeeeeeee esse nme ne
11. s B verage Wavelength nm 545 80 USB2 platform multi channel Average to All Wavelength nm isi 59 For USB1 when getSoft is acquiring data the edit fields are gray and non editable By clicking the red Stop button data acquisition is stopped and the edit fields become white and editable The edit bar shows the following parameters Integration time ms 200 00 Integration time ms This option changes the CCD readout frequency and therefore the exposure or integration time of the CCD detector The longer the integration time the more light is exposed to the detector during a single scan so the higher the signal If the integration time is set too long too much light reaches the detector The result is that over some wavelength range the signal extends the maximum counts 16383 or in extreme case shows as a straight line at any arbitrary height even near zero Entering a shorter integration time can usually solve this Try to adjust the integration time such that the maximum count over the wavelength range is around 15000 counts When at minimum integration the signal is still too high an attenuator a neutral density filter or fibers with a smaller diameter may be used When not enough light reaches the spectrometer likewise a longer integration time should be entered It is also possible to let getSoft search for a good integration time by clicking the AC button or by using the menu option Setu
12. 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 18 more than spectroscopy Gel spec com 3 Menu Options In sections 3 1 to 3 3 the main menu options File Setup and View Menu and their submenus are described in detail 3 1 File Menu PY GetSofbe amp Full 2006 GetSpec File Setup View Application Help Stare New Experiment on E ATI Save Ld Load 3 Average f Print Black and White printer Display Saved Graph Convert Graph Exit 3 1 1 File Menu Start New Experiment After selecting this option a dialog box appears in which a new experiment name can be entered The experiment name will be saved as a filename with the extension kon This extension does not need to be entered After clicking the Save button the ERTS TT TTT x current filename will be built up from su oae the experiment name that has been entered and a sequence number starting at 0001 Example if the experiment name is esktop test the first graphic file that will be 9 saved in scope mode will be called B j test0001 ROH the sequence Piet number will be automatically incremented so the next file that will eis be saved in scope mode will be XE aee mm ee called test0002 ROH etc For a detailed information on graphic aa Dess E filenames see 3 1 7 File Menu Sav
13. 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 16 more than spectroscopy Gel spec com Zoom out Drag with the left mouse button within the white rectangle but in stead of dragging the mouse downwards and to the right drag it in another direction After releasing the mouse button both the x and y axis will be reset to their default values Move X Y Dragging with the right mouse button results in moving the complete spectrum up or down and to the left or right Move Y If a mouse wheel is available on the mouse being used then the spectrum can be moved up or down by moving the mouse wheel 2 5 Status bar USB1 l aster File getSpec 0007 ARAH Amplitude 3830 3 Smoothing 1 Spline ON For each selected spectrometer channel a status bar at the bottom of the main window shows information about the file to which the data will be saved amplitude at current wavelength and the current settings for the smoothing and spline parameters The field at the right of the Spline setting is used to indicate that the spectrometer is receiving too much light at a certain wavelength range 2 16383 counts before correcting for dynamic dark smoothing or averaging in which case the label Saturated will become visible See also section 3 2 9 1 Setup Menu Options Check on Saturation USB2 TA
14. Always show this dialog opens this serial port You will need to enter the port number in this example COM8 later in getSoft Data communication has now been established which is illustrated by a line between the device and the PC By clicking the menu option Tools Configurations Quick Connect the COM port number that has been assigned for the data communication between the spectrometer and the PC can be verified Starting getSoft getSoft can be started after data communication Iul xl has been established The first time that getSoft SIE cen is started for the Bluetooth spectrometer it will CAU ee tae show the dialog at the right Select one ol the ophons below then click OF ce press Erber Click the option Retry to establish E RU ERE LR IUE T CE PERRA communication through RS 232 or Bluetooth A C Ciise gees list with available comports will be displayed In our example COM8 has been selected for the Bluetooth data communication Select the right COM port from the list and click OK getSoft will now read the configuration data from the spectrometer this can take about 15 seconds after which measurements can be started info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 8 Imore than spectrosco
15. CIE 1931 or 1964 Standard Observer value 2 or 10 degrees angle at wavelength The tristimulus values X Y and Z and the spectral irradiance are computed in a wavelength range from 380 nm to 780 nm using a 1 nm interval The CIE1960 UCS color coordinates u and v are calculated by 3 4x 5 6y U o V gt V 2x 12y 23 2x2 12y 3 The equation that is used for calculating the color temperature is empirical and assumes a black body radiator 9 x 0 332 P Ta 0 1858 ColorTemp 5520 33 6823 3 p 3525 p 449 p In LED measurements the Dominant Wavelength and Purity also known as Helmholtz coordinates are often used to describe a color The Dominant Wavelength can be calculated for a measured sample point S with chromaticity coordinates Sx Sy by drawing a straight line from the midpoint in the chromaticity diagram E with x 2 y 2 0 333 through S towards the edge of the Chromaticity Diagram spectrum locus The points at the spectrum locus correspond with a wavelength and the interception of the straight line through E and S with the locus is called the Dominant Wavelength info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 79 more than spectroscopy Gel Spec com Purity is the distance from the midpoint E to the samp
16. Instead the wavelength range that has been entered in the function definition box will be displayed at the x axis The spectrum for the selected spectrometer channel will be displayed in the selected measure mode In combination with getSoft XLS the View Spectrum mode can also be used to export the spectra online to Excel section 4 6 info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 58 more than spectroscopy Gel spec com If the History Channel Functions are used to track the intensity or wavelength of a peak see below for the Function Definition Peak a graphical feature can be used to visualize the peak changes in a spectrum The number of peaks to display in the spectrum can be entered This number between 0 and 100 is the number of vertical lines that will be drawn in the spectrum each vertical line representing one of the detected peaks in the past o The height of the vertical line is equal to the peak intensity o The wavelength at which the vertical line is drawn in the spectrum corresponds to the wavelength of the detected peak o The color of a vertical line represents the oldness of the peak Most recent peak is red going back in time followed by orange green and blue All older peaks are gray colored from light gray oldest peak
17. Settings dialog As long as the reference channel is the Active FOS Channel the data processing does not occur in the main window but a small graph as shown 400 500 EU 700 aui in the figure below shows the Wavelength nm Active FOS Channe Sample channel Reference channel Elapsed time since last FOS channel switch 0 00 38 info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 433 0 4 72 80 82 43 Fax 449 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 41 more than spectroscopy Gel Spec com A D Counts measured at the FOS reference channel When clicking the Sample channel radio button the FOS 2 switches channels and the corrected data will be processed as usual A small window will remain visible in which the FOS channel can be switched back to the FOS reference channel and measure a new reference spectrum If set to Auto switching of channels will be done after a preset number of seconds Furthermore you can Select if yOU want to C Continuously update FOS reference measure sample on switch update the FOS reference spectra once every IRR u E x seconds or only take one sample measurement once every x seconds In figure at the right the sample channel will be measured during most of the 60 seconds Once every 60 seconds the FOS switches to the FOS reference channel
18. The selected parameter in the radiometric graph is also used to determine the peaks in the peak measurement box The radiometric spectra can be saved and loaded by the Save and Open Saved Graph buttons see below under button bar Button bar The Exit button will close the irradiance chart and return to the aetSoft main window Ext Pause Info m idl Integration time m p Sad average fo The Pause button will stop data processing to be able to take a snapshot at the spectrum and or output parameters Power emitted ulate Power emitted nWatt The Info button shows information about the current settings as shown in the dialog left The last line in this dialog Save irradiance spectrum Yes refers to the save setting in section 4 4 5 1 Irradiance Chart Settings If this option is 450 500 560 600 650 70D enabled then all displayed parameters will be Expand Size Wavelength nm saved in this example to irrchart2 txt and the info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 94 I ore than spectroscopy Gel Spec com spectrum for the selected parameter in the radiometric graph will be saved to irrchart2xxxx irr in which xxxx represents the sequence number If the Save irr
19. allocate a spectrometer to an application for example if one spectrometer is running with getSoft Full and another C 031101451 spectrometer needs to run with getSoft Raman software f 020800681 But it can also be used to run multiple spectrometers simultaneously just by restarting getSoft multiple times After clicking the OK button getSoft will communicate with the spectrometer serial number that has been activated in the dialog USB2 platform a Setup Hardware E iol xj SerialNumber Detector Mr Of Pixels Ful Range nm Name Activated MasterSpre O603001U7 TCD1304 3648 350 0 to 1079 4 Toshiba a 60302607 SonyiL 554 aie 1773 ta T1000 Sorp e Iv Synchronize scans between all spectrometerz seESpec Jb48 mode PreScan mode d OK x Cancel C ClearBulter mode This option shows all connected spectrometers and allows you to change the name of the channel and to deactivate and activate spectrometers For synchronization of scans a master sync spectrometer needs to be dedicated in Multi channel platforms usually the spectrometer from the left as seen from the front is predestined as master sync by Sentronic info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 27 more than spectroscopy Gel
20. an intensity calibration can be performed By selecting the menu option Application gt Absolute Irradiance Measurement a window is shown in which the Perform Intensity Catbration settings for the intensity calibration become visible Petform Intensity Calibration after clicking the Perform Intensity Calibration Spectrometer Channel button The following settings need to be entered Master before starting the intensity calibration o Spectrometer channel Calibration Lamp CX getSpec6242000120 DH mp o Calibration Lampfile View Edit Lamp Data CEN AS Fiber or get Sphere ssmpleport diameter For a single channel spectrometer the spectrometer 10000 channel will always be set to Master For multi channel spectrometer systems the spectrometer l channel for which the intensity calibration will be 3tart Calibration save Calibration performed can be selected from the list Master iL olavel o CC UV VIS fiber or integrating sphere sample port diameter micron Calibration Lamp Select Lamp Calibration File xl Look mr 3 aetzcec D cf ee The energy output in uWatt cm nm for the calibration lamp that will be used can be found in a file with the extension Imp This file needs to be selected by clicking the Select button After the calibration file has been selected the data can be
21. 1 Exit Calculate New Calibration Line A new calibration is started by clicking the Start New Calibration Clear Data button All data fields for the selected spectrometer channel will be cleared Enter the wavelength for the measurements in the Peak at field and the width of the peak that will be used in the Width field The program will integrate the absorbance values from Peak minus Width to Peak plus Width and uses this integral as the absorbance value in the concentration calculations The unit of concentration can be entered in the Conc Unit field This is just a text no adjustments will be made if you change this value Enter the known concentration values for the available standards in the central column of the calibration points table You can include the reference sample with zero concentration in these measurements After this the dialog contains for example the data shown in the figure at the right Tel Fax 449 0 351 871 8423 449 0 351 871 8465 T l Fax oxi Select Variables Actual Points Channel Master Actual Line 5 m New Points Peak at nm w New Line p o Width nm 2 Cone Unit E Start New Calibration Clear Data CA Bi ie u TAA EE r Calibration Points Calibration Line Coefficients F First Order Linear Default Int t C Second Order Quadratic k Coef H1 Actual Calibration Filename Master Co
22. 11 54551 29 203 83 14 25478 3 204 19 12 54551 31 204 56 14 26478 32 204 93 16 54551 33 205 29 14 26478 34 205 66 16 54551 35 206 03 13 26476 I4 MN Graphi AvaSoft Data r Test sheet 4 Gereed INUM getSoft will check if a sheet named getSoft Data is present and will add this sheet if it is not present This way links to the sheet can be preserved between sessions The sheet contains the following data at fixed positions A3 Date Time stamp This is a floating point value in which the integer part is the day number starting at January 1 1900 with day 1 The fractional part represents a decimal time value where 0 5 is 12 00 h noon and 0 75 is 18 00 h This way differences in date time can be readily calculated by subtracting values which would be much more difficult if this was a text representation B3 Time in milliseconds after midnight This value does not have a one millisecond resolution Remember that Windows is not a real time operating system It can however be used as a reasonably accurate indicator of the time that passes between scans C5 C Wavelength scale in nm for first History Channel spectrum info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 104 Imore than spectroscopy Gel Spec com
23. 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 110 Imore than spectroscopy Gel Spec com ia 4 6 3 Enabling the application Enable the application by selecting Application gt Chemometry gt Enable from the main menu A checkmark will appear before the menu entry to show that the option is enabled A separate Concentration display will appear for each spectrometer channel enabled After enabling the chemometry application you can select Chem as a function type in the Function Entry dialog of the History application Tel Fax 449 0 351 871 8423 449 0 351 871 8465 loxi B mmol l 2 1 History Channel Function Entry User Defined Integral View Spectrum info getSpec com T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 111 more than spectroscopy 5 Help After clicking the menu option Help gt Contents the getSoft FULL manual will be displayed in HTML format At the left side the Help Contents displays all sections to which the user can browse to a specific topic Chemumrtry Application geaoit Chen was imini te mhk m i concen sion mahrini with rpeclrameser zyriem Joc or ding 3 Lambert ieer 2 bee there i a beer relationshap between abearbance md conzentratian A ce
24. 8 Perform scans continuously save first available spectrum as Reference if TTL on is high pin 8 automatically enable the v Automatically save on Hardware Trigger Delay from DOZ to start scan 1000 nanoseconds Automatically save on Hardware PNE dn i Ze E ance max 2730563 ns Trigger option Then click OK _X Cancel When measuring in external hardware mode the data acquisition is controlled by the external trigger pulse input Therefore the Start Stop button will be disabled The edit bar will show the current setting for the delay 1000 ns in the figure below EU Getsoft t O Full 2005 GetSpec File Setup View Application Help Start MBS SATII OM eS fe bi E Hegsntneeb Averse O Wavelength inmi fesser I Trigger Delay 1000 ns 000 As long as the spectrometer is in this mode you cannot send commands from getSoft to the spectrometer So if you need to change the delay you will first need to disable the external trigger mode This can be done in the external trigger mode dialog by selecting the None button but a shortcut with the same effect is to click the Trigger Checkbox in the edit bar of the main window Note that one more trigger pulse is needed before the external trigger mode is disabled The reason for this is that the spectrometer can only react on an external trigger at pin 4 before it can respond to the Disable External Trigger Mode command A
25. 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 102 more than spectroscopy memand faq feud Jecemem Oprek Exe Owa enter Dm REC BEAN OO Se Oi el cial mE B gel Spec E lE a ede sur ST TES algi eo 4 XN Ee eae E is Firon 11329 ORONO 11 3234 mOr 61 32 26 OM 103238 es OM WS a2 1 i 41522847 2554194 41554706 125500 41555025 1 555605 a 4 196 07 2814117432 3 eig A UC 3 26 COGOR UTE t XI 4 0 5 ran 35 D 2063431543 75311520 LTH TIOEINGS SOE Ss 32059055717 1 156 E 125 75 4 B341174 2 A FEDER GL ATO 2B 5 SORES TOUT 0 22M T SUT aad t313 7 ie t 4 Sere 043 4 364 112006 IEEE 3 342056574 3 UIT 3055 iOS ms et 8 ran 4 pur 17450 1 PRISER a CC 3726 D a5 169495 SGT oe 3 307 E3584 a OC 5 Ib HE 2 5331547 354115206 0 Fd Taso 2 43 5574 DUST ESS LIEN 0 156 10 1ST 22 D B34117422 2 224104 Ji ATOS4 37 2B Eun 4 722877500 4 ESP 16 156 Hi it 137 55 qoi 43 ESA er in D eoe o THES D 206055 7717 UE IET 12 rar 95 1 B241 17432 D zem 1529655378 D HOSS 0 72280 aoe T 2 EK 13 Lb BE E 5 TUBOS EATEN E 251504 5 ES Rae ae BSE 2 PATA 4 195 14 ISE ES 3 E5 INH LuNa B 437 EHE ETI 269761 HEE 15 ia rs HERI CR 1m 1 E HACER E 760 15504 4 BEER SOG RRS Ce SAL LF a 7o TE 5 19 16 138 4 10 toon 5 23041104 25655118 j 420 yea B T2287 7800 1 5071815 ga I 28 73 aS rupe 5 EAA B znegT5SOS B BSUS LHE TAANE B 1965
26. Gel spec com Saved Dark as shown in the dialog at the right which appears after clicking the FWHM option After clicking the OK button the Full Width Half Max values are calculated for a number of peaks To mark a peak for which the FWHM values need to be calculated press the ALT key and click with the left mouse button on this peak If only one spectrometer channel is enabled and there are no earlier saved graphs being displayed then the FWHM value of the marked peak will be shown directly on top of the peak Furthermore this peak will be yellow marked over the width of the FWHM value it may be necessary to zoom in on the peak to be able to view that the peak is marked If more than one spectrum is being displayed a dialog as shown below pops up in which the spectrum for which the FWHM needs to be activated can be selected out of all displayed spectra In the figure below peaks have been selected for FWHM calculation The FWHM Males are ave in the same color the spectrum is drawn in Brik ha Ga hi ea Boe Fal aJades H gll E qe r om bap eL ck zi I Hau HW dee OE Be Se AH aia 1 EITE a do To disable the FWHM calculation the menu option which is marked as long as the FWHM is enabled needs to be reselected 3 2 9 3 Setup Menu Options Integrals This utility can be used for measuring the total amount of energy coming into the spectrometer Up to 10 integrals can be displayed simultaneously The integ
27. OK button you can select the files which need to be converted Selecting these files is the same as for converting to ASCII without the equi distance feature Faces section 3 1 11 Also the information in the header file is the same as for converting to ASCII without the equi distance feature The data in the TRT TAT TTT and TIT files is presented in two columns The first column gives the equally spaced wavelength in nanometers The second column shows the interpolated value for scopedata in a TRT file absorbance in a TAT file transmittance in a TTT file or irradiance in a TIT file Distance between datapoints 1 00 nanometer info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 25 more than spectroscopy Gel spec com 3 1 13 File Menu Convert Graph to JCAMP JCAMP DX is a standard file format for exchange of spectra between different spectrometer systems and computers A JCAMP DX spectrum is a text file which can be viewed corrected and annotated with a text editor A lot of other elaborate spectrometer software programs like Grams32 and OPUS are capable of importing files in JOAMP DX format The JCAMP format requires an equal distance between the data points at the x axis wavelength Converting the CCD pix
28. Select the file and click the Open button 4 Switch to the Irradiance Chart TAB to enter the hardware setup and select the colorimetric radiometric photometric and or peak parameters of interest see section 4 4 5 1 Irradiance Chart Settings Then click OK 5 Measure the output parameters in the experiment If needed change the integration time such that the maximum in scope mode is around 14000 A D counts Block the light path to the spectrometer and save a dark spectrum If the ir radiance of the light to be measured needs to be displayed against time click the Time Measurement TAB in the settings dialog as described in section 4 4 6 Time Series Measurement 4 4 3 Load Intensity Calibration By selecting the menu option Select Intensity Calibration File Application gt Absolute Irradiance Zokenm Cudss G Measurement a window is shown in which the intensity calibration that il faim time Hamz5b LL isti 32 was saved before can be loaded by a eis EXDAIT20T Master UVVIS df clicking the Load button An H Nieuwe map 3 D2f 2t 5ave Vv 5 cfr intensity calibration file contains the smoothing 3 Mastemew df data which is necessary to convert I speed 2 SlavelNew dh the Scope data to Irradiance data If the spectrometer system has one or more slave channels it is important Bestandsnaann E2120 Master UVV15 dlr to Know that the calibration data for Bestandstypen Intensity Calibr
29. USB dongle A Bluetooth driver installation CD is included in the Tornado USB dongle package This section describes how to set up Bluetooth data communication with the getSpec Bluetooth spectrometer Install getSoft for USB2 spectrometers from the getSpec Product CD ROM Insert the getSpec Product CD ROM in the CD ROM drive of the PC select Software Installation and install getSoft Install Bluetooth driver Insert the Tornado Installation Disc into the CD ROM drive of your PC and follow the on screen instructions After the installation has been completed you will be asked to restart the computer After the restart the IVT BlueSoleil window is shown Insert the Tornado USB dongle Connect the 12 V DC external power supply or battery pack to the getSpec First make sure that the battery pack is completely charged If not connect the battery pack to the charger and let it charge until the LED on the charger starts to flicker trickle charge mode Now connect the battery pack to the getSpec and switch on the battery pack switch on the front side and switch on the getSpec to external power Search for getSpec Spectrometer Device TT ee Pai MARRON The green power LED at the front side E iemuesem cere Tos Deb of the spectrometer should be on Mundi cCumerab Service Window Ci Tab Irrangs Devices Fir mh n amare pnis Select the menu option Refresh Devices in the IVT BlueSoleil Main Window a
30. be applied to zoom in on an interesting time interval A description for the Print button can also be found in section 4 1 2 Since the History Channels Output file is in ASCII this file can be easily imported in a spreadsheet program like Microsoft Excel The data in the file can also be viewed with a text editor like Microsoft Word or with Notepad An example of the file format is given on the next page info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 63 more than spectroscopy Gel spec com History Channel Functions Measurement Report Measurements started at 29 06 01 Time 15 06 29 Integration time 3 ms Average 1 scans Description of the Functions FUNCTION 1 User Defined Function m 400 FUNCTION 2 User Defined Function m 500 Absorbance Mode FUNCTION 3 User Defined Function m 600 Transmittance Mode FUNCTION 4 User Defined Function m 700 Irradiance Mode FUNCTION 5 Integral Master Channel between 380 0 and 780 0 nm Multiplied with 1 000 Transmittance Mode FUNCTION 6 View Spectrum in Transmittance Mode No Data Saved FUNCTION 7 Not Activated ocope Mode FUNCTION 8 Not Activated Time oeconds F1 F2 F3 F4 F5 15 06 29 0 62 79 79 2 294E 3 100 8 74 11 40192 15 06 30 0 77 81 84 2 059E 3 100 9 74 17 40236 15 0
31. files click the Cancel button New in getSoft 7 is that displayed graphs can be deleted or oe properties of the displayed graphs such as line style or color or Edit Comment comments can be changed This is done by clicking with the right mouse button on the line in the graphical display A small line edit box will occur Borders Now the line can be deactivated or the line properties Ie isibe Style solid m can be changed as depicted in the border editor or Cal width i E the comments can be edited __ Sb oe I In the right figure two graphic files were selected in Round Cancel scope mode The comments that were saved with these graphs are displayed at the top of the graphical info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 23 more than spectroscopy Gel spec com pd Bafi Ti PT 1 Pel TWN Ged Signe a CC E mL region together with mig agen information about amplitude i i eee at current wavelength amp integration time it and smoothing s settings at the moment that the file was saved and the name of the graphic file If the active spectrometer channels e g Master have not been unselected with the view channel option the actual data for the activated channel s will be displayed in the same graph as the
32. fon TIN UE can be made to overwrite the Se fn M old color parameters with the new ones or to keep the old values for this productlD see right figure o The Select button can be used to select a new reference color from the database as described in detail on the previous page o Each time the Save button is clicked a new record is added to the Filename in the lower left corner One record contains the following fields Date Time Comments L a b h c X Y Z RefProductlD Lref aRef bRef dL da db dE Channel Illuminant info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 71 more than spectroscopy Gel Spec com and Observer The Comments field contains the text that is entered in the dialog that is shown after clicking the Save button 4 3 2 2 Time Series Color of Object Settings j nl xl LABChart Time Series Fi r r r rs re r re Spectrometer Channel Illuminant CIE Standard Observer Master L C a Function Display Settings NE C D 5 2 degrees 7B C D50 D55 C 10 degrees LER NE ae Lr mars I NNNM E l Ss oe gt oe we Display No Graphics To Speed Up Data Processing L H MH t C Save Function Output x Cance
33. i Calculate Power Energy Photon Counts received by detector surface Calculate Power and or Energy emitted by a source Teotropic pointsource assumed Distance from source to detector can be set while measuring Radiometric Parameters and wavelength range can be selected while measuring If the hardware setup is Source Inside Sphere the detailed setting for the radiometric parameters emitted power in un Watt or emitted energy in uJoule is done while measuring in the irradiance chart dialog The same difference in hardware setup determines which photometric parameters can be measured If the hardware setup is not Source Inside Sphere the llluminance in Lux can be measured The Luminous flux the photometric equivalent of radiometric Power can be measured at the diffuser surface but it can also be calculated how much lumens are emitted by the source An important assumption is that the source should be an isotropic point source In this setup the Luminous Intensity in Candela can be measured as well If the hardware setup is Source Inside Sphere only the Lumens emitted by the source can be measured Photopic Parameters Hecaerved by detectar surface luminance Lus Lumens mr Luminous Flux Lumens Luminous Intensity Candela Emitted b Donde depuis Luminous Flux Lumens satrapic pointsaurce assumed Distance fram source to detector can be set while measuring pj The peak parameter group allows
34. in mols or as in our application in uymols The number of photons per nanometer can be calculated from the wavelength dependent photon energy and the absolute light energy that is measured A detailed description how this is done can be found at the next page The photon count distribution umol s m nm shows the photon flux received per square meter Other photon count units that can be calculated from this are o umol s nm photon flux received at diffuser surface o umol m nm photons received per square meter during one integration cycle o umol nm photons received at diffuser surface during one integration cycle info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 81 more than spectroscopy Gel spec com How to convert a power distribution uWatt cm nm into a photon count distribution uMol s m nm Photon energy E h c e Where h Planck s constant 6 626 068 76 x10 c velocity of light 2 998 x10 m s wavelength in meters For example the photon energy at 250 nm and at 1000 nm is E 250 6 626 x 10 2 998 x 109 250 10 7 946 x10 Joule photon E 1000 6 626 x 10 2 998 x 109 1000 10 1 986 x10 Joule photon 1eV 1 60207 x 10 Joule so the photon energy expressed in eV photon becomes E 250 7
35. is dynamic Note that this option is different from the dark current that needs to be saved before any transmittance or absorbance measurements can be taken File gt Save Dark If the correct for dynamic dark option has been changed it will be necessary to save a new dark and reference spectrum because the raw data has been changed If this menu option is preceded by a checkmark the scope data is corrected with the dynamic dark algorithm It is recommended to leave this setting checked which is the default state info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 30 more than spectroscopy Gel Spec com 3 2 5 Setup Menu Subtract Saved Dark This option is used to subtract the dark spectrum that has been saved File gt Save gt Dark from the raw scope data After starting getSoft this menu option is always unselected because a dark spectrum needs to be saved or loaded before it can be subtracted If this menu option is preceded by a checkmark the scope data is corrected with the saved dark 3 2 6 Setup Menu Strobe Enable This option can be used to enable or disable an external strobe e g the getLight XE attached to a getSpec 2048 spectrometer The measured light intensity of the getLight XE is independent of the in
36. is shown in which the new integration time is given Since the integration time has changed new reference and dark spectra need to be saved before switching to transmittance or absorbance mode is possible For this reason this option is only available in scope mode 3 2 9 9 Setup Menu Options Use Custom Reflection Reference In Transmittance Reflectance Mode the transmittance reflectance at pixel n is calculated using the current sample reference and dark data sets in the following equation sample dark ref dark 9 UE EE Get SS pee T C 3 f LT Getsofte m O Fail l xi Cn is the Custom Reflectance JN ma SATI IHSG wk E E Sweet B imam Em o eat j frma Reference factor at pixel n In earlier versions of getSoft this factor was set to 100 for each pixel and could not be modified In getSoft version 6 the custom reflectance reference factor is default set to 100 but can be set to different values if needed If the reflectance spectrum of a white calibration tile is known the data can be read from a file or entered in a table in getSoft The resulting custom reference data can info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 48 more than spectroscopy Gel spec com be save
37. is the cause of saturation o Zoomed in Saturation can also happen at a wavelength range that is not visible because the graph is not at full scale Under all these circumstances the saturation label will be shown in the status bar of the spectrometer channel for which one or more pixels are saturated During time series measurements History Channel Functions color versus time irradiance versus time the saturation detection will be done only for the relevant wavelength range The relevant wavelength range in color measurements is from 380 to 780 nm For History Channel Functions the relevant wavelength range is set by the user For example when monitoring an integral between 500 and 505 nm saturation detection and notification will be done only for this range If saturated the saturation label will become visible in the History Channel output If the time series measurements are saved online to Excel the font color in Excel will be red for a history channel as long as the output for that channel is saturated USB1 platform only Following options are available for USB1 platform spectrometers only information about the saturated wavelength ranges can be visualized and or saved by enabling the following options Additionally more detailed information can be retrieved in the following ways Visualize saturated pixels areas in graph Log saturated pixel areas to screen Log saturated pixel areas to file on Save Measurement
38. ln P PUT Mia AA M Lane TN bh re y y y M n I functionality as in the xdi iin inne j main window The Info button shows the Function Entry dialog in which all parameters can be viewed not edited while the measurement is running 1 By clicking the Print button the graphics that are displayed can be printed during the run First the printer specific dialog shows up in which the print options can be set For example if only one graph of the four in the figure above need to be printed this graph can be selected by the page s radio button All graphics are printed full size on a different page Zoom features In each graph the same zoom features apply as in the main window except for scaling the y axis with the mouse wheel However zooming in over the x axis while the measurement is running and the x axis is already scrolling will not be possible because in that case the x axis is updated with each new scan Clicking the Pause button to take a snapshot will solve this problem Zoom in Select a region to be expanded to the full graph To select this region click the left mouse button in the white graphics region and drag it downwards and to the right After releasing the left mouse button both the x and y axis will be rescaled to the new values of the selected region Zoom out Drag with the left mouse button within the white graphics region but in stead of dragging the mouse downwards and to
39. noise during the intensity calibration If a good reference signal is displayed click the white Save Reference button in the dialog above A white line will mark the reference spectrum Then switch off the calibration lamp wait until the spectrum becomes flat near the bottom of the scale and click the black button to save a dark spectrum A black line will mark the dark spectrum 4 4 4 2 Save Intensity Calibration If reference and dark data have been saved in the figure above the intensity calibration can be saved by clicking the Save Calibration button A dialog shows up in which the current E save Intensity Calibration ini xi settings in this intensity calibration are Calibration ight source used in this calibration shown If the calibration has been example mo performed with diffuser the intensity Select fiberoptic setup used in this calibration calibration data will be saved to an ASCII file E Itera Eulbrater perfmed van base bm with extension dfr with bare fiber this jnna Calibration performed with dilusa extension will be fbr The name of the E intensity calibration file can be entered after FieDifuee daseimSampie Pos ISP S00 micron clicking the Save As button For USB2 Parameters in this intensity calibration platform spectrometers the intensity Spechamelerchannek Master Integiationtine DIT milisecond calibration can be saved to EEPROM by Smoothing T peek clicking the Save To EE
40. o getSpec 2048FT or getSpec 2048F T y In which y represents the number of spectrometer channels O O OO The getSpec USB2 group of spectrometers should be selected to install getSoft7 for one of the following spectrometer types getSpec 102 256 1024 2048 3648 NIR256 USB2 Instructions below are for the getSpec USB1 and USB2 spectrometers Bluetooth instructions are found in section O 1 Installation Dialogs Choose Destination Location Em X The setup program will check the system configuration of the computer If no problems are detected the first dialog is the Welcome dialog with some general information Setup val metal getere USES imn He follosang Folder o anita ito a differed bolder check Brovee and select ari nkder fou can choose nea bo stall getiSpec LISB2 be ckckong Cancel In ial Setup In the next dialog the destination directory for the getSoft software can be changed The default destination directory is CAgetSOFT7USBA If you want to install the Destination Fakes software to a different directory click the ee minm Browse button select a new directory and click OK If the specified directory does not Cane info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 449 0 351 871 8465 Fax 433 0 4 72 80 82 43 Fax 449 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 5 more than spectro
41. option displays the hardware settings measurement parameters and save settings for the spectrum as shown in the dialog at the left The measurement parameters displays the unit of the intensity of the displayed saved spectrum This can be a different unit than the selected unit for the online measured spectra Also if comparing two saved spectra graphically be aware that these were saved in the same radiometric unit The other menu options in the popup menu Convert to ASCII and Convert to Excel can be used to export the data from the binary file with extension IRH to a textfile txt or to a file that can Es setings us be opened with Excel xls ET Irradiance Chart Measurement Info m E m x Spectrometer simulation Channel Master D etectarT ype CE LIVA vIS ar Fiber Diameter CC UV VIS or Fiber 2300 micron The Remove from Chart menu option clears the saved spectrum Lalibration settings Intensity Calibration Filename 0411058514 IS FC UVv200 2 sR CC At the right side of the button bar the integration time Integrationtime during calibration 213 ms Scelta dima E and number of scans to average can be changed The o Auto Configure Integration Time button can be used meenacswediumms to let getSoft search for integration time such that the E iradiance spectum No maximum output in scope mode A D Counts will be around 14500 counts Do not forget to save a new da
42. selected graphic files By clicking the green Start button the online measurements can be compared directly to the graphics that were saved before Vis aT200E ol Mpix 1277 scettr BOO pine OY The menu option File gt Display Saved Graph is preceded by a checkmark as long as the earlier saved graphics are displayed To clear these earlier saved graphics select again the menu option File gt Display Saved Graph after which the checkmark disappears and only the spectra for the active spectrometer channel s will be displayed 3 1 11 File Menu Convert Graph to ASCII This option requires that graphic files were saved earlier by using the option File gt Save Experiment After choosing this option a window shows all files in the current measure mode For instance if the measure mode is scope so the extension of the earlier saved spectra is roh To select graphic files that were saved in another measure mode e g absorbance click B behind the Graph Mode and pick the desired measure mode To select graphic files from another folder or drive click zl behind the current folder name If a graphic file is marked by a single mouse click on the filename the comment line for this file appears at the top of the graphical region in the main window Selecting multiple filenames can be realized by using the CTRL or SHIFT key in combination with the mouse If the CTRL key is pr
43. signal is high pin 8 External Hardware Trigger perform a single scan at rising edge of TTL pulse pin 4 Perform scans continuously save first available spectrum if TTL signal is high pin 8 In this mode getSoft will poll the status of pin 8 after each arriving scan If the status of pin 8 is HIGH 45V the spectrum or spectra in case more spectrometer channels are active will be saved automatically as described in section 3 1 7 File Menu Save Experiment A comment dialog will not be shown instead the spectrometer channel number and a time stamp will be written to the comment file Note that the spectrum will be saved only if the signal is high at the moment of polling which is the moment that getSoft receives a new spectrum from the data acquisition driver If a short TTL pulse is generated at pin 8 there is a info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 449 0 351 871 8465 Fax 433 0 4 72 80 82 43 Fax 449 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 43 more than spectroscopy Gel spec com big chance that the spectrum is not saved To save one spectrum the duration of the TTL signal should therefore be as long as the product of integration time and number of scans to average In time critical situations it is recommended to use the External Hardware Trigger Mode Perform scans continuously save first available s
44. spectrum with some heavily saturated peaks the auto correct can be used If one or more pixels of a spectrometer channel are saturated the text label saturated appears in the GetSoft main vandow statusbar for that channel Additionally more detailed information can be retneved in the Following ways To illustrate this a strong peak from the getLight CAL calibration line source was heavily saturated at 435 84 nm This caused the most heavily saturated pixels to return inverted 16383 counts pixel values figure below at the left In the right figure the saturation detection has been set to the third level which will not only detect the saturated pixels at 16383 counts but also detect and correct the inverted saturated pixels Disadvantage of the auto correct detection level is that processing the saturated scans by the application takes more time Visualize saturated pixels areas in graph Log saturated pikel areas to screen Log saturated pixel areas to file on Save Measurement X Cancel Mitsa cheated pcos eae Master channel Scope Values Saturation Notification If the saturation detection has not been switched off the spectrometer channel status bar will show the text label Saturated as long as one or more pixels of that spectrometer channel are saturated Slavel File gts001 ROH Amplitude 261 50 Smoothing 0 Spine OFF Saturated Master File gts002 ROH Amplitude 3613 7 Sm
45. the Grid Enable option is activated a grid will be displayed in the graph as shown in the figure below ET GetSsofto ZO Full 2005 GetSpec File Seis ges Awxraben Hels w PRS SATI IHtihh E E migration tme yea ioni average lz vaneen gii nv ap 325 E BD E Eh Fie HEXMOD E ROH earpiece 7 5 00 Bare ere Sere OE lames 3500 008 6 Averaaes 2 3 3 11 View Menu Progress Bar Enable If using long integration times or a high number of averages it can take a few or more seconds before a new scan is received by the application To get an indication about how much time it will take until the next scan arrives a progress bar can be displayed After enabling the progress bar by clicking the menu option it will be displayed after the next scan has arrived The progress bar will be shown only if the time between scans is more than one second The time between scans is roughly the integration time multiplied with the number of averages However if the number of averages is high the time between scans can get longer because of the overhead time that is spent on transmitting the high number of average spectra to the PC info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 53 more than spectroscopy Tel Fax 449 0 351 871 84
46. the right then click em the Save button AS a result My Documents the file MyFirst cal will be created and the calibration e line will become the actual ac calibration line that will be EE Filename iFirst El My Network P used to calculate the Save astype Calibration files cal Cancel 2 concentration levels for the specified spectrometer channel Master in the example After a calibration line is E chemometry Settings E ini x saved it will become the lasso S Ace Parts actual calibration line which EM NN peers is blue colored This actual n seo mm f f idt 10 nm line will be used for En SERRE 0 0 2 0 4 0 6 0 8 Start New Calibration Clear Data X M caen i Tel 49 0 351 871 8423 T l 3 pipes nud pid r Calibration Points Calibration Line Coefficients First Order Linear Defau Fax 49 0 351 871 8465 Fax Conc 0 C Second Order Quadratic um Intercont 7 3811E 001 0 000 0 633 Coef 81 gg768E 001 30 000 29 342 Coef H2 0 0000 000 70 000 69 146 St Dev 1 07538 95 000 95 813 Actual Calibration Filename Master MyFirst cal Load Read Absorbance for point 2 f Exit more than spectroscopy Gel spec com concentration calculations Also the calibration line coefficients are shown in the lower right corner of the dialog and the predicted concentration values that hav
47. the right drag it into another direction After releasing the mouse button both the x and y axis will be reset to their default values Move X Y Dragging with the right mouse button results in moving the complete spectrum up or down and to the left or right info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 62 more than spectroscopy Gel Spec com 4 1 3 History Application Display Saved History Graph If time measurement data have been saved EFM EAE the resulting ASCII file can be displayed aa WB graphically by selecting this file in the dialog that is shown after clicking the Display Saved History Graph menu option After clicking the Open button the function os is output of the functions with function type Integral or User Defined is displayed against time Both x and y axis are set to Bestandsnaam History dat their full scale which IS determined by the Bestandstypen History Channel Datafle Annuleren minimum and maximum values in the list Mearaemards tartsd wt TET TTEA HRS LM Ee lul E m NM pani mm MAN AW Tike l m Mh demi T lu mm fi li ad nin m uhi TAH Lad Hl i The zoom features as described in section 4 1 2 History Application Start Measuring can
48. time Depending on the maximum counts in the last scan the integration time will be increased decreased automatically until a scope signal of about 14500 counts is measured During the search routine the changes in integration time can be followed in the integration time edit box in the edit bar A dialog will display the new integration time if the search is ready The routine can be aborted by clicking the button again before the search has ended H C F button The History Channel Function button allows you to switch directly to the history channel function screen to start measuring immediately Of course first the functions need to be defined The two buttons below are available only if getSoft has been ordered together with the color application add on iz Color Chart button The Color Chart button allows you to switch directly to the color chart screen and starts measuring immediately r Lor UL Color of Object versus Time button The Color of Object versus Time button allows you to switch directly to the time measurement of color parameters and starts measuring immediately info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 433 0 4 72 80 82 43 Fax 449 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 14 more than spectroscopy Gel Spec com 2 3 X Edit bar USB1 platform Integration time ms
49. to dark gray one peak older than blue The time interval at which the vertical lines are updated is the same as the time interval at which the function output is saved to be entered under Save every x Seconds under Save Function Output For example suppose the function output will be saved every 3 seconds and the number of peaks to display has been set to 10 in function F1 After starting the history channel measurements the peak in the spectrum drawn for F1 will be marked by a red vertical line immediately In the next 3 seconds the spectral peak may vary in height or position but the red vertical line remains at the same place and height After 3 seconds the red vertical line becomes orange and a new red line will be drawn at the position and with the height of the peak detected at that moment t 3 After 6 seconds another line is drawn and the colors are shifted again so that red is most recent then orange then green At t 27 seconds the 10 vertical line is drawn again red for the most recent the oldest is now represented by a light gray color At t 30 seconds the oldest peak is removed from the list since in this example the Nr of peaks to display is 10 a new one is added and the colors are shifted according to the definition etc Function Definition Peak Function Definition Spectrometer Channel Wavelength range nm Peak Qutpu Master From 455 0 To 45110 v Wavelength Intensity In case a
50. to take a new FOS reference spectrum that will be used for the correction After clicking OK in the FOS Settings dialog the FOS will display one of the two dialogs below As long as the sample channel is active left dialog the measurement results will be processed as usual As long as the reference channel is active right dialog the measure ment results will be used to update the reference spectrum and the A D Counts of this spectrum will be displayed in the dialog FOS Ref Channel FOS Sample Cha n Time left until next FOS channel switch jo 00 25 Time left until nest FOS channel switch fo 00 27 500 550 B00 650 O0 750 Wavelength nm Correct for Drift by Spectrometer Channel USB1 platform only This option is available if the spectrometer system has one or more slave channels One spectrometer channel will be used as a reference channel which will continuously measure the reference spectrum e g the white tile in reflectance measurements or the cuvette holding the TEREE reference solution in transmittance measurements Changes in this slave a reference signal e g because of drift in the light source will be used to correct the data of the other selected spectrometer channels The Correct Channels wavelength range over which the data can be corrected will be the overlapping wavelength range between reference spectrometer channel and the spectrometer channel to be corrected I
51. which parameters will be A eenia shown in the irradiance chart By clicking one of the group deu aD ANTE parameters the detailed settings for this group become visible A re 77 7 description about these parameters and how they are calculated Changs Daip Fia can be found in section 4 4 1 Background secs Imstlbines Speer By default only the Colorimetric parameters are enabled CIE Standard Observe f 2 degrees Colorimetric Parameters x u Dominant wavelength v B v Complementary Dom Wavelength E i olor Temperature Purity 10 degrees The parameters that are enabled in the figure above will be displayed in the Irradiance Chart info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 90 more than spectroscopy Gel spec com The detailed setting for the radiometric parameters is done while measuring in the irradiance chart dialog If the hardware setup is not Source Inside Sphere you can already select if you want to measure only radiometric parameters received at the diffuser surface or also calculate the power and or energy emitted by the source An important assumption when calculating the emitted power or energy is that the source should be an isotropic point source Radiometric Parameters
52. will be called F1 for history channel 1 F2 for history channel 2 etc If extra sheets are added a suffix will be added to this name e g F1_01 F1_02 etc If the function type View Spectrum has been selected for more than one History Channel Function e g Master channel for F1 Slave1 channel for F2 then the different history channel sheets named F1 and F2 are located in separate workbooks and must be saved under separate filenames This is also the case for the sheet with 8 History Channel values described before Rows 1 and 2 contain the date time stamp and the elapsed millisecond value as described in the previous section Column A of the first sheet holds the wavelength scale The wavelength range matches the wavelength range as specified in the History Channel Functions The lower left half of the Settings dialog can be used to enter the number of scans you want to export to Excel and the time interval in seconds between two scans you want to log Default values are 1000 scans and no interval meaning as fast as possible Continuous Mode You can select continuous mode by selecting the radio button Continuously overwrite data to a fixed position in an existing workbook in the Select menu In this mode data will be written to a single sheet each scan overwriting the previous one info getSpec com Tel 49 0 351 871 8423 T l Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 02 Tel 433 0 4 72
53. window After starting the irradiance measurements by clicking OK the output of the selected parameters will be displayed against time If the option Display no graphics to speed up data processing has been marked in the function entry dialog the function output will be shown by numbers only which are updated each time a new scan is saved to the output file There are six buttons at the top of this window see figure below an Exit button a Pause Start button a Save Reference button a Save Dark button an Info button and a Print button If the red Exit button is clicked the time measurements are ended and the main window and menu is activated The yellow Pause button can be used to stop the time measurements temporarily After clicking the Pause button data acquisition stops and the text on the button changes to a green Start If the Start button is clicked data acquisition is activated again and the text changes back to the yellow Pause again The Save Reference and Save Dark buttons have the same functionality as in the main window The Info button shows the dialog in which all parameters can be viewed not edited while the measurement is running By clicking the Print button the graphics that are displayed can be printed during the run First the printer specific dialog shows up in which the print options can be set For example if only one graph of the four in the figure above
54. you to enable disable the following peak information Peak Parameters Center Wavelength nm fw Centroid nm Jf Absolute Peak nm FWwHM v Amplitude at Center Wavelength w Amplitude at Centroid v Amplitude at Peak Detailed settings like the wavelength range and the radiometric output parameter for which the peak s are calculated can be done in the Irradiance chart dialog Save Settings Save experiment results to File irrehart tt Change Output File Sawelradiance Spectrum The Graphics setting determines which graph will be displayed in the irradiance chart dialog The Save Settings are used to enter the name of the text file to which the results of the irradiance chart measurements will be info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 91 more than spectroscopy Gel Spec com saved By default this name is irrchart txt but by clicking the Change Output File button this filename can be changed By enabling the Save Irradiance Spectrum option a complete irradiance spectrum will be saved together with the parameter results The name of the irradiance spectrum file will start with the filename that has been entered irrchart in the figure at the right followed by a sequence number and
55. 0 info getSpec com Tel 49 0 351 871 8423 T l 433 0 4 72 80 82 02 Tel 449 0 231 974 2285 Fax 449 0 351 871 8465 Fax 433 0 4 72 80 82 43 Fax 449 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 73 more than spectroscopy Function Independent Parameters Display Ma Graphics To Speed Up Data Processing Save Function Qutpu Do not save Function Output Save every 0 Seconds Save Function Output To File D test CoaT ime dat W Create Backup File d OF x Cancel Save Settings Load Settings Function Independent Parameters Display no graphics to speed up data processing Below the TAB sheets for function definition an option can be enabled or disabled to speed up data processing by not displaying the graphics during the measurements If an application requires fast data processing this option should be enabled If saved to an output file the data can be displayed graphically after the time series experiment has been ended as described in section 4 1 3 History Application Display Saved History Graph Function Independent Parameters Save Function Output The results of a time series color experiment will be saved to an ASCII file if the radio button Save Function Output has been selected A number of seconds between saving can be entered for data reduction in case measurements are carried out over long periods Entering a value of zero results in saving
56. 0 380 430 480 530 580 630 680 730 780 Wavelength nm The three categories that were defined for the radiometric parameters can also be used for the photometric parameters o The photometric equivalent for the Radiant Flux Watt is the Luminous Flux expressed in Lumens o The photometric equivalent for the Radiant Intensity uWatt sr is the Luminous Intensity expressed in Lumens sr This unit is equal to Candela o The photometric equivalent for Irradiance uWatt cm is called Illuminance expressed in Lumens m This unit is equal to Lux oince the geometry of the three categories is the same for radiometry and photometry the same can be written about the hardware setup luminous flux can be measured inside an integrating sphere When measuring a source at a certain distance from the integrating sphere or cosine corrector the luminous flux can be calculated assuming that the source is an isotropic point source The Luminous Intensity Candela and Illuminance Lux of a sphere can be measured outside the integrating sphere or with a cosine corrector info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 84 more than spectroscopy Gel spec com 4 4 2 Quick Start Quick Start 1 Perform absolute irradiance measurements using a calibrated lam
57. 03 2002 11 32 36 41556317 O 11 52971 0 0 0 0 0 0 11 07 03 2002 11 32 37 41556616 0 15 38815 0 0 0 0 0 0 12 07 03 2002 11 32 37 41556926 0 14 91846 0 0 0 0 0 13 07 03 2002 11 32 37 41557239 O 11 53747 0 0 0 0 0 0 14 07 03 2002 11 32 38 41557539 O 22 45974 0 0 0 0 0 0 15 07 03 2002 11 32 38 41557850 O 18 35447 0 0 0 0 0 0 15 07 03 2002 11 32 38 41558150 O 19 098817 0 0 0 0 0 0 17 07 03 2002 11 32 38 41558450 QO 13 05762 0 0 0 0 0 0 18 07 03 2002 11 32 39 41558771 D 12 70234 0 0 0 0 0 0 19 07 03 2002 11 32 39 41559091 0 17 02149 0 0 0 0 0 0 20 07 03 2002 11 32 39 41559382 0 16 68021 0 0 0 0 0 0 21 07 03 2002 11 32 40 41559692 16 61596 0 0 0 0 0 0 22 07 03 2002 11 32 40 41560005 O 23 0465 0 0 0 0 0 0 23 07 03 2002 11 32 40 41550313 D 17 72162 0 0 0 0 0 0 24 07 03 2002 11 32 41 41560614 O 22 07574 0 0 0 0 0 0 25 07 03 2002 11 32 41 41560924 QO 22 14798 0 0 0 0 0 0 26 07 03 2002 11 32 41 41561235 0j 23 95353 0 0 0 0 0 27 07 03 2002 11 32 42 41561535 O 16 97764 0 0 0 0 0 0 28 07 03 2002 11 32 42 41561646 0 21 8558 0 0 0 0 0 0 29 07 03 2002 11 32 42 41562156 O 22 19735 0 0 0 0 0 0 30 07 03 2002 11 32 42 41562467 D 21 85271 0 0 0 0 0 0 31 07 03 2002 11 32 43 41552757 D 17 15362 0 0 0 0 0 0 32 07 03 2002 11 32 43 41553087 O 20 67904 0 0 0 0 0 0 33 07 03 2002 11 32 43 41553388 O 20 19298 0 0 0 0 0 0 34 07 03 2002 11 32 44 41553588 D 17 33064 0 0 0 0 0 0 35 07 03 2002 11 32 44 415639
58. 18 200 Te 7 1Bg5gccoeH EISH Sasa 4 B 427 pad GS mP pje nr EESFIEIS gas 19 200 5 RICE im 11 SoReal panca 1055758415 7 OE OE B outs T 13 15 2a 200 10 ae 14 20d ER FIT 159405 T2207 70800 Ei 156 gni Fi AX Xx aT BOO Eie i ers ID OB BHACTS Mcr ern In 0424475 LIE Er 201 6 ESE 10 2354 104 10 52855527 13 487 15247 CaP nl nr 165716156 B0 81 a Ai 5E 1i 7150806 7 ESSR m TE TEC h BESO pe ee eur LT B rogi Th 13 15 2d XO X 9 185960568 B 2504104 11 6205580 7 B aoo 95 9 TII TOO 1 68216 156 1181 a XL 15 71350005 otii 13 27581585 Ie nro 18 SS In zd p E Lu E 203 m 11 rm 13 2 24 114 3 53055827 aor Sa RTIIHPTEOD T EST16 156 l Prj Xn as 13 71450806 TERE 18 77pcs 105575843 12 0660026324 BD Toutsd TES B0 1 Am 23 85 13 30531 45s 71 2304 104 14 6205587 B 437 59495 12 2x05 ti 5H 15k gm iH AA m 15 7 14540008 EEn i 14 2763155 B ES ae TAS ee TE n 18 a Jas 13 Sees 15 2354 104 14 S2055827 1z 47 BSS 1272287775 13 8215 156 au gt Au g3 15 771450808 15 S835 BUD if PESTS 16 5575843 15 oe 1 704944765 13 13 x ENS 14 noa 13 22241104 18 5 059567 14 407 165053 EMEA ni 1 216 166 1 Ii 206 Ix 13 7 1450008 ITA 14 En 14 55 033417 gXLSIM 13 7 0842414705 H 1s H 260 12 eS 11 23 11 52B55b27 12 48718245 a 722807 0CG 1465216156 11 97 35 06 35 13 7 14508906 1 235583 13 27581655 n eom o6 2 goce 174 13 794504 75 i5 12 ud Hoa HNE FID wa Sy Since there is a maximum of 256 columns per sheet new sheets will be added after column 256 IV is filled The first sheet
59. 23 449 0 351 871 8465 info getSpec com T l 33 0 4 72 80 82 02 Tel 449 0 231 974 2285 Fax 33 0 4 72 80 82 43 Fax 449 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 54 more than spectroscopy gel Spe ccom 4 Applications FY GetSolbe E Full 2006 GetSpec File Setup View Application Help Gtart IN History il Wavelength Calibration b Frocess Control b Absolute Irradiance Measurement Excel Output b Oxygen b Chemometry b 4 1 Application History Channel Functions 4 1 1 History Application Function Entry With the History Application the output of Fi r2 rs r rs re F e self defined functions or Function Type integrals can be followed Mone Integral User Defined View Spectrum 7 Peak in a graph against time i M Mad Up to 8 fu nctions can be C Scope i C Absorbance v Transmittance C radiance followed simultaneously mm The functions are defined Spe komete Chenn Wavelength range nm Multiply with aster F From To in the dialog at the right M t pon poo i ogg which is shown after the menu option Application Function Display Settings manis time r asig Function Value gt History gt Function Auto Fixed Auto Fixed Seconds Entry has been chosen T ee ee Min 5 i T Max The functions F1 to F8 sce amp can be selected by Display No Graphics To Speed Up D
60. 286 Dresden Lyon Dortmund Sofia Seite 65 Imore than spectroscopy Gel Spec com ia coefficients Select OK and the new calibration will be applied immediately 9 Atleast 3 peaks are needed to successfully complete a new calibration Try to calibrate with more peaks Selecting too many peaks can however lead to peaks that cannot matched with standard wavelengths 4 2 2 Wavelength Calibration Restore Original Calibration This option enables the user to restore the calibration coefficients to their original values i e the values that getSoft was shipped with If a new calibration was performed with a limited number of peaks or over a limited wavelength range the results could be less favorable This option allows the user to undo unwanted changes to the calibration 4 3 Application Color Measurement L 64 40 Chamnel Hastsr 76 38 Tllwminamti 45 b 30 34 bsrrwrr 7 degreesm eo 33 18 f 3 90 E 5 5 H 325 483 C 06 81 The getSoft Color Application has been developed to perform on line color measurements with a spectrometer system It can be used for reflective color measurements in earlier versions of getSoft called Color Of Object Measurements The CIE 1976 L a b color parameters are calculated as well as other frequently used parameters like Hue Chroma and X Y Z These parameters can be displayed in a CIELAB chart or in a graph versus time It is also possible to sav
61. 31 974 2286 Dresden Lyon Dortmund Sofia Seite 20 more than spectroscopy Gel spec com 3 1 6 File Menu Save Reference With this option reference data are saved For USB1 platform the name of the reference data file is ref dat where represents the number of the slave channel for which the reference data has been saved 0 represents the master channel For USB2 platform the name of the reference data file is serialnr ref The reference data files will be saved in the experiment directory that has been picked by the option File gt Load gt Experiment or File gt Start gt New Experiment 3 1 7 File Menu Save Experiment With this option spectral data is saved All graphic files will be saved in the experiment directory that has been picked by the option File gt Load gt Experiment or File gt Start gt New Experiment Saving graphic files if one spectrometer channel is enabled First a window appears in which a line of comments can be entered to the saved graph Than two files will be saved the first file contains the saved spectrum data The name of this first file starts with the experiment name directly followed by the sequence number of the saved spectrum The extension of this first file depends on the current measuring mode as shown below Extension Mode ROH Scope Mode ABS Absorbance TRM Transmittance Reflectance IRR Irradiance The second file co
62. 4 micron will cover 15 14 210 micron at the CCD array Using a fiber diameter of 200 micron means that we will lose resolution when setting the smoothing parameter to 7 Theoretically the optimal smoothing parameter is therefore 6 The formula is slit size pixel size 1 2 In the table at the left below the recommended smoothing values for the getSpec 2048 getSpec spectrometer are listed as function of the light beam that enters the spectrometer This light beam is the fiber core diameter or if a smaller slit has been installed in the spectrometer the slit width Note that this table shows the optimal smoothing without losing resolution If resolution is not an important issue a higher smoothing parameter can be set to decrease noise against the price of less resolution c 102 getSpec getSpec getSpec getSpec 256 1024 NIR256 Pixel 25 um Pixel 14 um Pixel 8 um Pixel 50 um getSpe slit fiber Pixel 77 um 3 2 44 Setup Menu Correct for Dynamic Dark getSpec 2048 3648 only The pixels of the CCD detector getSpec 2048 3648 are thermally sensitive which causes a small dark current even without light exposure To get an approximation of this dark current the signal of the first 14 optical black pixels of the CCD detector can be taken and subtracted from the raw scope data This will happen if the correct for dynamic dark option is enabled As these 14 pixels have the same thermal behavior as the active pixels the correction
63. 6 30 0 92 81 79 3 756E 3 100 2 73 77 40201 15 06 30 1 07 84 69 3 209E 3 101 0 73 43 40202 15 06 30 1 22 82 19 7 877E 4 100 3 74 11 40188 4 2 Application Wavelength Calibration Please refer to section 3 2 2 Setup Menu Wavelength Calibration Coefficients for a description about the polynomial that converts the pixel number on the detector into the corresponding wavelength at the x axis in getSoft lf a Mercury Argon light source getLight CAL is available together with suitable optical fibers an automatic wavelength calibration can be performed The recommended optical fibers used for auto calibration are GS SMA IR 008 2 8 um core Vis NIR 2 meters long SMA GS SMA IR 050 2 50 um core Vis NIR 2 meters long SMA GS SMA UV 050 2 50 um core UV Vis 2 meters long SMA An important precondition for a successful auto calibration is the absence of saturation The easiest way to assure this is to select the Check on Saturation option section 3 2 9 1 setup Menu Options Check on Saturation If the soectrometer is saturated at minimum integration time a fiber with smaller core diameter e g GS SMA IR 008 2 needs to be used info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 64 more than spectroscopy Gell Spec com As an al
64. 65 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 98 more than spectroscopy Gel spec com a value of zero results in saving every scan The name of the file to which the data will be saved can be changed after clicking the Change Output File button ES RE E s Last option in the Save Function Output box is to enable or disable the possibility to create a backup file during the measurements If this option is enabled getSoft will create a backup file with the same filename but with the extension bak also in ASCII This backup file is updated every scan and can be used in case the filename that has been selected has failed to save the data for instance because of a power failure during the measurements Save ICF Load ICF buttons getSoft automatically saves all parameters irradiance parameters save options to the file irrtime62 ini and restores these parameters the next time getSoft is started With the Save ICF and Load ICF buttons it is also possible to save these parameter settings to and load from a file with the extension icf With this feature it is possible to save for each experiment a different icf file so it can be loaded a next time the same settings are required After the definition of one or more functions the OK button is clicked to start measuring the Cancel button to leave the dialog and return to getSoft s main
65. 71 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 82 more than spectroscopy get Spec com Hardware Setup outside sphere or CC outside sphere or CC outside sphere or Radiant CC outside sphere or CC outside sphere or CC Intensity outside sphere or Energy CC received Power emitted Energy emitted Radiant Flux Power emitted Energy emitted Radiant Energy Radiant Flux uWatt Total optical power emitted from a source uJoule Total optical energy emitted from a source calculated by multiplication of the power with the integration time uWatt Total optical power emitted from a source calculated by multiplication of radiant intensity with the solid angle of the light source UJoule Total optical energy emitted from a source calculated by multiplication of the power with the integration time uWatt sr Optical power per unit solid angle calculated by multiplication of irradiance with the square distance between point source and diffuser surface UJoule sr Total optical energy emitted from a source calculated by multiplication of the radiant intensity with the integration time uWatt Power received at diffuser surface UJoule Energy received at diffuser surface calculated by multiplication of the power with
66. 80 82 43 Fax 449 0 231 974 2285 449 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 103 more than spectroscopy gel Spec com In the lower right part of the Settings dialog you can select the workbook the sheet will be added to thereby enabling you to use your own calculations and graphs in Excel on live data from getSoft Click the Select File button to select the workbook Please save an empty workbook using Excel if you do not have a previous workbook to open Ed Microsoft Excel continuous xls Cars B Fw 5 c S I B m 81 xj Bestand Bewerken Beeld Invoegen Opmaak Extra Data Venster Help 16 x OSE eT X3 oA A d C vas 10 Bru mesmp sxuwu 5 A Q19 m z A B E D E E G H J K IL M N a 1 Time Elapsed msecs YVL1 F1 WL2 F2 WLS F3 WLA F4 WLS5 F5 WLS FB el 07 03 2002 11 27 32 41252018 0 20 0438 4 5 Please do not enter your own 195 01 0 73522 B formulas in this sheet 195 38 0 545509 7 las they will be overwritten 195 75 3 73522 8 by AvaSoft 196 12 3 45449 E 195 48 0 73522 10 196 85 0 545509 11 197 22 1 264776 12 197 59 2 545509 13 197 95 0 73522 140 198 32 5 545509 15 198 69 7 254778 lig 199 06 4 545509 17 199 42 7 264776 18 199 79 12 54551 200 16 7 264778 20 200 52 11 54551 21 200 89 9 264778 22 201 26 10 54551 23 201 52 13 26478 24 201 99 13 54551 25 202 36 11 26478 26 202 73 11 54551 27 203 09 7 264778 28 203 46
67. 9 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 92 more than spectroscopy gel Spec om candela need to be calculated then the distance meter and geometry of the lamp steradians can be entered in the irradiance chart window as well The geometry of the point source is default 4p stera Power emitted hatt iant and luminous flux calculations 2 500 meter 12 566 srteradians indow will be described Experiment setup Distance from poin 3 Geometry of the po 2 1 l f M c In this section the followir s00 550 BD 650 O Add Edit radiomet Expand Size Wavelength nm o Graphics o Button bar The other output parameters are displayed according to the settings in the irradiance chart settings dialog More information about all output parameters can be found in section 4 4 1 Background Add Edit Radiometric Parameters There are two ways to monitor the radiometric parameters The first one is by defining up to ten different parameters or wavelength ranges in the radiometry box at the right side of the irradiance chart window The second one is by selecting a radiometric parameter in the graph after which the spectrum for the selected parameter will be displayed see below under graphics To define an output parameter at one of the ten lines in the radiometry box sy double click the line or click the Edit button on top E Radiometric Parameter Selectio Oo x and enter
68. 946 x10 1 60207 x 10 2 4 9592 eV photon E 1000 1 986 x10 1 60207 x 10 2 1 2398 eV photon Suppose we measure 20 Watt cm at a certain wavelength 20 Watt cm 20 Joule s cm 0 2 Joule s m 0 2 1 60207 x 10 eV s m 1 248 10 eV s m S Knowing the photon energy at 250 nm and at 1000 nm from 3 and 4 the number of photons that correspond with 20 Watt cm at 250 nm and at 1000 nm can be calculated from 5 by for 250 nm photons 1 248 10 4 9592 2 517 10 photons s m for 1000 nm photons 1 248 10 1 2398 1 007 10 photons s m With 1 mol 6 02308 x10 Number of Avogadro 1 mol 6 02308 x10 So the number of photons expressed in mol s m when measuring 20 Watt cm at wavelength 250 nm and also 20 Watt cm at 1000 nm becomes for 250 nm 2 517 10 6 02308 10 0 418 mol s m for 1000 nm 1 007 105 6 02308 10 1 672 mol s m In the table below the radiometric parameters that can be measured in getSoft are listed Note that a wavelength range needs to be specified over which the parameter spectral output will be integrated In the first column Hardware Setup inside sphere refers to measurements that are done with the light source inside an integrating sphere while outside sphere or cc refers to measurements that are done with a light source at a certain distance from the sphere or with a cosine corrector info getSpec com Tel 49 0 351 8
69. 99 O 24 58224 0 0 0 0 0 0 I4 4 gt M NAvaSoft Data Gereed z The sheet will be called getSoft Data the columns contain the following data Column A contains a data time value formatted as dd mm yyyy hh mm ss This is a floating point value in which the integer part is the day number starting at January 1 1900 with day 1 The fractional part represents a decimal time value where 0 5 is 12 00 h noon and 0 75 is 18 00 h This way differences in date time can be readily calculated by subtracting values which would be much more difficult if this was a text representation Column B contains a time value representing the elapsed milliseconds since midnight This value does not have a one millisecond resolution Remember that Windows is not a real time operating system It can however be used as a reasonably accurate indicator of the time that passes between scans Columns C through J contain the 8 different History Channel values For unselected History Channels a value of 0 will be entered in the sheet If you select View Spectrum in your choice of History Channels the sheet will be formatted vertically As Excel only offers 256 columns per sheet we need to write a spectrum which can hold more than 2000 pixels in a column instead of a row and add more columns as time passes info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231
70. Autoscale The Autoscale option will set the y axis range to the minimum and maximum function values that are in the list of measured data points Function Independent Parameters Display Ma Graphics To Speed Up Data Processing Save Function Qutpu Do not save Function Output Save every E Seconds Change Output File Save Function Output Ta File D Sw BSFLILE peaktrack sdatasrrT ime dat J Create Backup File J X Cancel Save ICF Load ICF Function Independent Parameters Display no graphics to speed up data processing Below the TAB sheets for function definition an option can be enabled or disabled to speed up data processing by not displaying the graphics during the measurements If an application requires fast data processing this option should be enabled If saved to an output file the data can be displayed graphically after the time series experiment has been ended as described in section 4 1 3 History Application Display Saved History Graph Function Independent Parameters Save Function Output The results of a time series color experiment will be saved to an ASCII file if the radio button Save Function Output has been selected A number of seconds between saving can be entered for data reduction in case measurements are carried out over long periods Entering info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 84
71. B1 platform only seeeeseeeeeeeeeeeeeeese PWM getLight LED control USB2 platform ussssse OD TONS Garces sere E E E DD NERD LOO oPPIETUEUMIUE Setup Menu Setup Menu Setup Menu Setup Menu Setup Menu Setup Menu Setup Menu Setup Menu Setup Menu Options Check on Saturation cccccccccec ec eeececeeeeeeaeeeseeeeaeeeeeenes Options Full Width Half Max ccccccccceceseeeeeeeseeeeeeeeeeeeeaeeeeeeaes Options INC ON NS aeeoea A EEEE Options Autosave Spectra Periodically Options Correct for Drift USB1 platform only Options Automatic Save Dark by TTL shutter Options External Trigger Setting cccccececsceeceeeeeeeeeseeeaeeeeeeees Options Auto configure Integration time eseseeeseeeeeees Options Use Custom Reflection Reference setup Menu Options Suppress Save Comments sss 449 0 351 871 8423 449 0 351 871 8465 info getSpec com T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 2 more than spectroscopy Gel Spec om 3 2 9 11 Setup Menu Options View Reflectance instead of Transm
72. Display settings The measurement results can be displayed graphically in 3 charts 1 the LABChart in which a and b are shown by a moving dot in a CIELAB graph 2 the Measured Color Panel which displays the color which is being measured Note that the displayed color on the monitor may not match exactly the color of the object depends on monitor but it will give a good impression 3 The Reference Color Panel which displays the reference color that has been selected under the reference color setting The graphical display can be enabled or disabled by a mouse click for each graph separately o Display following Color Parameters The values of the color parameters that are enabled will be displayed below of the charts during the measurements o Save Settings The color parameters can be saved to an ASCII file during the measurements The current name and location of this ASCII file is shown under save setting This filename and path can be changed by clicking the Change Output File button The next dialog will be displayed To start a new file for saving the color parameters enter a filename that does not already exists getSoft will add the txt extension and click Open If an existing file is selected the color parameters that will be saved are appended to this file This way experiments that were saved before can be proceeded using the same Bestandsnaam test but output file Bestandstypen Color of Object Output F
73. Guide What Is VBScript Language Reference Ce FileSystemObject User s Guide Adding VBScript Code to an HTML Page c Scripting Hun Time Reference Q Regular Expressions Guide vBScript Fundamentals VBScript Data Types VBScript variables VBScript Constants VBScript Operators Using Conditional Statements Looping Through Code VBScript Procedures Finally the third Help submenu is the about box which shows some information about the getSoft version that is being used the serial number of the spectrometer that is connected the Windows version of the computer and the available memory info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 113 more than spectroscopy Gel Spec com Appendix A Troubleshooting How to rectify an incorrect USB installation Any USB device requires that the USB drivers need to be installed before the device is connected to the computer If you accidentally connected the getSpec 2048 spectrometer to the USB port of your PC before installing getSoft the USB drivers cannot be found and the spectrometer cannot be found by the later installed software To rectify an incorrect installation follow these steps Action View 1 Navigate to the Device Manager If you have rj HERR i jus Computer
74. M Data Acquisition Devices 4 Disk drives a Display adapters 5 CVDICD ROM drives 4 amp 3 Floppy disk controllers 4 Floppy disk drives SS IDE ATA ATAPI controllers qu Keyboards Mice and other pointing devices 3 Monitors BI Network adapters Ay Ports COM amp LPT ls C Ec Er Ec Ec Ec EH Ee Ed EH EH EH Windows98 select Start Settings Control Panel Double click the System icon Select the Device Manager tab For Windows2000 systems right click on My Computer select Properties select Hardware tab and click on Device Manager button scroll down until you see Other devices Under Other devices you will see USB device with a large question mark hit the remove button or right click the USB device and select the uninstall option Ef Sound video and game controllers T Ml System devices 4 A warning box will appear to ask if it is OK to BE uc nor EE Ed 5 Unplug the getSpec 2048 spectrometer from pep aot Do your PC and reinstall the getSoft software niversa Host Controller 6 Now you can plug the getSpec 2048 spectrometer to the USB port again Do not forget to connect the getSpec 2048 to the 12V power supply as well Windows will display the Found New Hardware USB device dialog and starts searching for the driver this can take a few minutes Depending on the remove the device click OK E Avantes Spectrometer board Windows version the Files Needed E es dialog at the right ma
75. OS A Fie get Spec DOZROH Amplitude 617 40 Smoothing Spline OFF intime 6 37 ms Averages 1 158 For each selected spectrometer channel a status bar at the bottom of the main window shows the name label of the connected spectrometer channel the file to which the data will be saved amplitude at current wavelength the current settings for the smoothing and spline parameters the line color and style in the graphical display the real integration time and averages per channel and final the number of scans taken since the Start button was clicked The field at the right is used to indicate that the spectrometer is receiving too much light at a certain wavelength range 2 16383 counts before correcting for dynamic dark smoothing or averaging in which case the label saturated will become visible 2 6 Find peaks or valleys by CTRL or SHIFT left mouse button click This option can be used in all modes Scope Absorbance Transmittance or Irradiance and for all displayed graphics When the left mouse button is clicked in the graphical region while the CTRL key is down getSoft will follow the following procedure to run to the closest peak 1 The wavelength is determined from the position the mouse click occurred 2 he data from closest pixel is retrieved 3 he direction to search for the peak is determined from the neighbor pixels If both neighbor pixels have a lower value at the y axis than the current pixel the current pixel is a
76. PROM button The following data will be saved to the intensity calibration file Save Ta EEFHOM Save To Pie As i o Name of light source calibration file Imp o Spectrometer channel 0 Master 1 Slave etc o Integration time in milliseconds o Diameter of diffuser surface in microns o TOTAL PIXELS 2048 for getSpec 2048 values which represent the dynamic range reference minus dark data at each pixel during the intensity calibration divided by the intensity of the calibrated light source that was used o Setting for the Smoothing parameter during the intensity calibration After an intensity calibration has been performed a graph is displayed which shows the data transfer function for the spectrometer channel The irradiance spectrum is calculated by multiplying the measured scope data from which a saved dark spectrum is subtracted with this data transfer function info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 89 more than spectroscopy If the intensity calibration has been performed the Irradiance Chart and Irradiance Time Series tabs will be enabled Clicking one of these tabs allows you to change the settings for the irradiance chart or time series measurements after which the measurements can be star
77. Save Settings Save experimental results to file C AvaSpec60 data 02080035 1 stickers txt Change Output File r Reference Color gt DB File D ASourceXS W amp Delphi amp xexample pct Current Reference Color Select Select Current Reference Color from T able LREF 64 42 aREF 70 38 bREF 50 56 Add Add Current Reference Color to T able ProductID red sticker 00000000 M Add New Reference Colors to DB when measuring X Cancel The color measurements in the LABChart are started by clicking the OK The following parameters can be set before the measurements are started o Spectrometer Channel If the spectrometer system contains one or more slave spectrometer channels the spectrometer channel on which the color will be measured can be selected from the drop down box at the upper left corner showing by default Master Make sure white reference and dark spectra have been saved before for the selected spectrometer channel o Illuminant The CIE Standard sources A B or C or one of the illuminants D50 D55 D65 or D75 can be selected by clicking one of the radio buttons The default is D65 which is the most widely used illuminant It represents the power distribution of average daylight with a correlated color temperature of 6500 K The D50 D55 and D75 have correlated color temperatures of 5000 K 5500 K and 7500 K The CIE Standard sources A B or C represent respectively I
78. Spec com getSpec 3648 USB only The Toshiba detector as implemented in the getSpec 3648 can be used in two different control modes 1 The Prescan mode default mode In this mode the Toshiba detector will generate automatically an additional prescan for every request from the PC the first scan contains non linear data and will be rejected the 2 scan contains linear data and will be showed on the screen and or saved This prescan mode is default and should be used in most applications like with averaging only one prescan is generated for a nr of averages with the use of an getLight XE one or more flashes per scan and with multi channel spectrometers The advantage of this mode is a very stable and linear spectrum The disadvantage of this mode is that a minor 5 96 image of the previous scan ghost spectrum is included in the signal This mode cannot be used for fast external trigger and accurate timing since the start of the scan is always delayed with the integration time min 3 7 ms 2 he Clear Buffer mode In this mode the Toshiba detector buffer will be cleared before a scan is taken This clear buffer mode should be used when timing is important like with fast external triggering The advantage of this mode is that a scan will start at the time of an external trigger the disadvantage of this mode is that after clearing the buffer the detector will have a minor threshold in which small signals 500 cou
79. a info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 100 Imore than spectroscopy Gel Spec com This is done in the same way it would be done without the Excel output option with the input screens you use to define History Channel Functions and Time Series in the Color and Irradiance applications The only difference is the Save Function Output box which is not used by the Excel Output option This part is replaced by a separate dialog which will be described under Settings 4 5 2 Enable Excel Output Enable the option by selecting Application gt Excel Output gt Enable A checkmark will appear before the menu entry Enable to show the status of the option 4 5 3 Settings getSoft will write the data to three differently formatted sheets depending on user input in History Channel Function Entry and the Settings dialog You can enter the Settings dialog by selecting Application gt Excel Output gt Settings E excel Automation BE Bl xl Select Made Export a fized number of scans to Excel Continuous Made Workbook Select File Export Made Scans to log fi O00 Interval sec 0 First select the mode you want Export Mode You can select the Export Mode
80. a few hundred maximum because a large number of files in the same experiment directory have a negative effect on the system speed If the automatic saving needs to be aborted before all spectra have been saved the Autosave Spectra Periodically menu option can be selected again and the value 0 can be entered for the number of spectra to be saved info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 38 more than spectroscopy Gel Spec com Save on Digital IN USB2 platform only For the USB2 platform the DB26 T p A save Parameters IB x connecior pins 7 16 and 24 may m be used to connect external The DB26 connector has 3 digital inputs pin 7 16 and 24 IF a high TTL signal is recereed at these pins spectra can be saved p witch h h witch switches such as photo switches or a new reference p 15 or dark p 24 spectrum can be stored to save a spectrum reference or dark This is specially useful for automated sampling in a process control environment with periodical Spectrometer Spectrum Reference Dark updates of dark and reference 3648 7 signals It is recommended to enable the Automatic save dark by TTL shutter in combination with the Save on digital In Dark setting Save Periodically Save Un Digital IN
81. adiance spectrum option is not enabled only the displayed output parameters will be saved By clicking the black Save Dark button a new dark spectrum will be saved It is recommended to save a new dark spectrum each time after changing the integration time Clicking the Save fj irrchart2 txt Notepad j File Edit Format Help Data button will add IRRADIANCE MEASUREMENT RESULTS the measured data to Spectrometer pete Channel ee 13 8 2004 14 58 21 DetectorType Avasphere source inside sphere the text file An user Mi ied sampleport AvaSpere 10000 micron Smoothing parameter 6 comment Can be added Irradiance Spectrum saved to D Sour ce sw6_ Delphi6Xdata 021200652XIRRCHART20004 IRR User comments yellow led S N 030812344 jr to the saved data If a Yee eee Measurement results veeeer ix wee colorimetry radiometric spectrum DW 572 276nm JUS A 97 44 has been saved with epic the output parameters feiatintin Photonetry 05369 Lumen the name of this file is wienn Radiometry Power Ce from 380 00 to 780 00 nm 94 057 ywatt i wwwwwwwwww peak Measurements from 380 00 to 780 00 nm also saved in the text Full width Half Max 31 421 nm fil center wavelength 568 36 nm He Intensity at CWL 2 3515 Te nm Peak wavelength Intensity at Peak centroid wavelength Intensity at Centroid m m m m Ww I un uw aon cn lad gt 2 3742 watt nm 2 2677 watt The Display Saved Graph button
82. ame result can be achieved with the option View gt Irradiance Mode ii Auto scale y axis button By clicking this button the graph will be rescaled on line A maximum signal will be shown at about 75 96 of the vertical scale The same result can be achieved with the option View gt Autoscale y axis info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 13 more than spectroscopy Gel spec com E Change Graph Scale button By clicking this button a dialog will be shown in which the range can be changed for both x and y axis This range can be saved as well and restored any time by clicking the Goto Preset Scale button see below The menu option with the same functionality is View gt Change Graph Scale Goto Preset Scale button By clicking this button the scale for x and y axis will be set to a range that has been set before The same result can be achieved with the menu option View gt Goto Preset Scale Eg Graphic Reset button By clicking this button the x and y axis will be reset to their default values The same result can be achieved with the option View gt Graphic Reset Auto Configure Integration time button After this button is clicked getSoft starts searching for an optimal integration
83. an be used to display data real time or to take a snapshot ih lt Cursor button After clicking the Cursor button a vertical line is displayed in the graph If the mouse cursor is placed nearby this line the shape of the mouse cursor changes from an arrow to a drag shape If this shape is displayed the left mouse button can be used to drag keep left mouse button down the line with the mouse towards a new position Moving this line shows the corresponding values of wavelength and amplitude in the main screen As an alternative for dragging the line the Set Big Step Cursor Movement EJ small step and big step arrow buttons may be used Or the Set cursor movement in nm far lt lt and gt gt left and right arrow keys on the keyboard The step size for 5 OI the arrow buttons can be changed by holding down the CTRL key while clicking at a single or double arrow button __ Caneel E Save reference and dark buttons The Reference button is the white button at the left top of the screen It needs to be clicked to save the reference data The same result can be achieved with the option File gt Save gt Reference The Dark button is the black button at the left top of the screen Click it to save the dark data The same result can be achieved with the option File gt Save gt Dark info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax
84. annel If the spectrometer system contains one or more slave spectrometer channels for which also an intensity calibration has been loaded or performed the spectrometer channel on which the output parameter will be measured can be selected from the drop down box at the left showing by default Master o Parameter The number of available parameters and the way these are calculated depend on the hardware setup The figure above shows the settings that are available when measuring with a cosine corrector or with an integrating sphere at a certain distance from the light source For each function F1 F8 one of the 35 available parameters can be selected See section 4 4 1 Background for further information about these parameters The colorimetric parameters can be calculated by using the 2 degrees standard observer values or the 10 degrees If one of the info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 97 m ore than spectroscopy gel Spec com radiometric parameters is selected the wavelength range needs to be specified The wavelength range is the spectral range over which the radiometric output will be integrated If the source is not inside an integrating sphere the radiant intensity in uWatts sr and radiant energy in uJoule sr can be calculate
85. annel while the top channel should be connected to the optical path that is used to measure the samples The Correct for Drift with the FOS 2 can be done in two different ways 1 Byregularly switching to the FOS reference channel the measured spectrum can be compared to the saved FOS reference spectrum The differences can be used to correct the data that is measured at the FOS sample channel To be able to correct the sample data the following data needs to be available o Dark spectrum FOS sample channel o Dark spectrum FOS reference channel if integration time at reference channel is different from integration time at sample channel o Reference spectrum at FOS reference channel 2 n Transmittance Reflectance or Absorbance mode the measured spectrum at the FOS reference channel can be used as a new reference for the FOS sample channel To be able to correct for the difference in sensitivity between both FOS channels the reference material white tile or blank needs to be saved at the FOS reference channel as well as at the FOS sample channel This option requires an equal integration time for FOS reference and sample channel The following data needs to be available o Dark spectrum at FOS sample or reference channel o Reference spectrum FOS sample channel o Reference spectrum at FOS reference channel The difference of these two methods of correction is that in the first method there is no relation between the data measured
86. are not in the wavelength range where the user is interested in Saturation can usually be solved by selecting a shorter integration time When at minimum integration the signal is still too high an attenuator a neutral density filter or fibers with a smaller diameter may be used info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 32 more than spectroscopy Gell Spec com In getSoft different levels of saturation detection can be set and there are also different options for notification as shown in the figure at the right Saturation detection levels The default level of saturation detection is Detect Saturated Pixels at saturation level Only for getSpec 2048 spectrometers the third auto correct inverted pixels level is available The reason for this is that if the detector type in the getSpec 2048 Sony ILX554 is heavily saturated at a light intensity of approximately 5 times the intensity at which saturation starts it will return values 16383 counts The other detector types in the getSpec 102 256 1024 and 3648 do not show this effect so no correction is needed Normally you do not need to use this third F7 saturation Detection Set Saturation D etectiorr C Off level for the getSpec 2048 but when measuring a peaky
87. at both channels getSoft uses only the differences measured in time at the FOS reference channel to correct the data measured at the sample channel In the second correction method the correction factor between the both FOS channels is determined first and then the white reference measured at the FOS reference channel is recalculated and saved as a new reference for the FOS sample channel oince for both methods a dark spectrum needs to be available save dark before enabling the Correct for Drift by FOS 2 INLINE UV VIS menu option After clicking the menu option the following dialog will be shown info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 40 more than spectroscopy FOS Settings reference channel 17 000 16 000 E Integration time ma Far FOS reference channel 15 000 4 5 fio Mr af scans ta Average for FOS reference channel 14 000 4 13 000 4 Save Dark for FOS reference channel 12 000 4 14 000 Save Reference for FOS reference channel w 10 000 5 9 000 FOS correction made anon i C Software user controls FOS switch frequency F 7 000 f Auto FOS switch frequency is preset 6 000 5 000 4 000 3 000 2 000 1 000 f Continuously measure sample update FOS reference on switch Contin
88. ata The fifth column shows the calculated value for absorbance in a TAT file or transmittance in a TTT file The format for the irradiance text files TIT depends on the availability of getSoft IRRAD The absolute irradiance text file that will be generated if getSoft IRRAD is available shows six columns The first two columns are the wavelength and dark data The third column is the transfer function from counts to microwatts The fourth column contains the measured counts In column five and six the calculated irradiance values are given in microwatts per square centimeter column 5 and in photon counts column 6 The relative irradiance text file that will be generated if getSoft IRRAD is not available shows five columns The first column gives the wavelength in nanometers The second to fourth column give respectively the dark reference and scope data The fifth column shows the calculated value for relative irradiance 3 1 12 File Menu Convert Graph to ASCII Equi distance This option requires that graphic files were saved earlier by using the option File gt Save Experiment After selecting the option File gt Convert Eexport to ASCII equidistance satin Graph gt To ASCII Equi distance the wavelength range for which the data should be converted and the Start export at Wavelength SME distance between two successive data points can be cq eum LI entered in the dialog as shown at the right After clicking the
89. ata Processing clicking the Save Function Qutpu corresponding TAB sheet Do not save Function Output UE every 40 SESS _ Change Output File h t f thi di Save Function Output To File D sources SW 5 Delphid data sHistory dat at t e op O IS Ala Og I Create Backup File Furthermore a number of general function Cancel Save HCF Load HCF independent parameters can be entered below of the function TAB sheets Function Type The first time that the Function Entry dialog is activated all 8 functions are not activated Function Type None To define a function the Function Type radio button needs to be changed from None to Integral User Defined View Spectrum or Peak After defining the Function Type the Measure Mode Function Definition and Function Display Settings can be set info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 55 more than spectroscopy gel Spec om Measure Mode The four possible modes Scope Absorbance Transmittance or Irradiance are described in section 3 3 1 to 3 3 4 Note that if the selected measure mode is Absorbance Transmittance or Irradiance a reference and dark spectrum needs to be saved or loaded before the History Channel Measurements can be started Function Definition The para
90. ation Files e each spectrometer channel are saved in a separate file To measure irradiance data at more spectrometer channels simultaneously the calibration files for each spectrometer channels needs to be loaded first After loading an intensity calibration file a graph is displayed which shows the data transfer function for the loaded channel The irradiance spectrum is calculated by multiplying the measured scope data from which a saved dark spectrum is subtracted with this data transfer function info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 86 Imore than spectroscopy Gel Spec com k Irradiance Settings If the intensity calibration has been loaded the Irradiance e Series Chart and Irradiance Time Series tabs will be enabled E aU Clicking one of these tabs allows you to change the settings Load for the irradiance chart or time series measurements after Eraser cians E chorro which the measurements can be started by clicking the OK button The irradiance chart and time series settings are described in sections 4 4 5 Irradiance Chart and 4 4 6 Beim Intensity Calibration 4 4 4 Perform Intensity Calibration If a calibrated light source such as the getLight HAL CAL or getLight DH CAL is available
91. bs are both converted to JCAMP the result will be gts0001 dx in both cases 3 1 14 File Menu Exit Closes getSoft USB2 With the USB2 spectrometers the measurement settings are saved to EEPROM before the software is closed During the process of saving it is very important that the spectrometer is not powered off A dialog as shown at the right will become visible as long as data are saved 1 sec for USB2 about 8 sec for RS232 Bluetooth connection Ti tosing get Soft E ioj x Y 99 l Saing settings to EEProm Please do not power off until get Soft is closed info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 26 Imore than spectroscopy Gel Spec com 3 2 Setup Menu PY GetSofti 2OFvll 2006 GetSpec File Setup View Application Help Cla Hardware Wewelength Calibration Coefficerts Inte Smoothing end Spline w Correct For Canam Dark Subbract Saved Dark Strobe gettight xE Contra Fii i qetLight LED Control Options 3 2 1 Setup Menu Hardware USB1 platform This menu option displays a list of getSpec spectrometer serial numbers that are connected to the PC s USB port s uu nix and COM port s and which are not used by another mE instance of the application This option can beusedto a c
92. by selecting the radio button Export a fixed number of scans to Excel In this mode a predefined number of scans will be logged to new worksheets that Excel will open Depending on your choice of history channel one of two formats of sheet or both will be opened by Excel If you select regular history channels without selecting View Spectrum the sheet will be formatted horizontally with the program adding a new row for each measurement written info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 101 more than spectroscopy Gel Spec com Ed Microsoft Excel Bladi amp ca cafe ER KS pen BRI 81 xl Bestand Bewerken Beeld Invoegen Opmaak Extra Data Venster Help la x DHASAR BB o r 5 M C vs 10 B Zz u 88a E 5 A Al Time rn D E F G H J K L M Time IElapsed msecs F1 F2 F3 F4 F5 F6 F7 F8 2 3 07 03 2002 11 32 34 41553984 15 55232 0 0 0 0 0 0 4 07 03 2002 11 32 35 41554535 O 21 97153 0 0 0 0 0 0 5 07 03 2002 11 32 35 41554775 O 15 77341 0 0 0 0 0 0 6 07 03 2002 11 32 35 41555095 O 14 52785 0 0 0 0 0 0 07 03 2002 11 32 35 41555386 0 15 37158 0 0 0 0 0 0 8 07 03 2002 11 32 36 41555696 0 15 29554 0 0 0 0 0 0 9 07 03 2002 11 32 36 41556007 0 18 36336 0 0 0 0 0 0 10 07
93. can be clicked to load a radiometric spectrum that was saved before In the open file dialog the radiometric spectra that were saved before will be listed By single clicking a file name the comment for this file will be displayed in the graph By double clicking the file name or by clicking the Open button the radiometric spectrum will be displayed This can be repeated for a second filename which allows you to compare the output of the two spectra graphically The online measured spectrum will be displayed in the same graph and can be compared to earlier saved spectra as well Power emitted Watt v IRRCHART20005 IRR greenyellow IRRCHART20006 IRR orange 8 520 540 560 580 600 620 640 660 680 700 720 740 760 780 800 Wavelength nm info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 95 m ore than spectroscopy Gel Spec com The filename and comments for the displayed spectra appear on top of zur the graph the text colors are the same as the colors used to draw the Convert to ASCII spectra By clicking the blue or green filename with the right mouse Convert to Excel button a popup menu for this radiometric spectrum is presented with the menu options shown in the figure at the right Remove From Chart The Show Info menu
94. centration A c l Where A is the absorbance or extinction e is the extinction coefficient of the compound to be measured c is the concentration and is the optical path length In practice this relationship is only linear at reasonably low absorbance levels say less than 2 The wavelength at which the absorbance is measured must of course be kept constant To take concentration measurements you will need a spectrometer with either a cuvette holder or a dip probe and a suitable light source If you use cuvettes remember that glass cuvettes will absorb UV light If your wavelength is in the UV range use quartz or polystyrene ones instead getsoft Chem can display and save the calculated concentration in two ways o The concentration can be displayed on line in a separate display window for up to eight spectrometer channels if available o You can select up to eight history channels that can display and save concentration values against time This application can be combined with the Excel and Process Control applications 4 6 1 Quick Start How to make concentration measurements with getSoft Chem 1 Start the getSoft software and click the Start button in the main window 2 Setup the absorbance experiment with a cuvette holder or a dip probe measuring the sample with the lowest concentration usually your reference Switch on the light source 3 Adjust the integration time to get a good reference signal with a ma
95. cf ini and restores these parameters the next time getSoft is started With the Save HCF and Load HCF buttons it is also possible to save these parameter settings to and load from a file with the extension hcf With this feature it is possible to save for each experiment a different HCF file so it can be loaded a next time the same settings are required The left figure below shows the dialog in which the name of the hcf file can be entered after clicking the Save HCF button The right figure below which is displayed after clicking the Load HCF button illustrates how to select an earlier saved HCF file Enter History Channel Function Definition Filename Opslaan als a speed experiment 1 hcf Zoeken in speed EN T experiment 1 hcf e experiment 2 hcf e esperiment 3 hcf e New het fe al Bestandsnaam experiment 4 Opslaan als type Function Detinition Files Annuleren Bestandsnaarn experiment 1 hcf Bestandstypen Function Definition Files Annuleren After the definition of one or more functions the OK button is clicked to confirm the Cancel button to leave the dialog without changes If the OK button is clicked getSoft performs a number of checks on the data that has been entered If no warnings show up the parameters that have been entered are accepted and the measurement can be started by the Application gt History Sta
96. ch demonstrates linking to the getSoft Data sheet In this case two graphs are drawn from the columns for Channel 1 and 2 The graph and data are continuously updated while data is being transferred to Excel info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 105 p i el spec com more than spectroscopy g Ed Microsoft Excel continuous xls a e i Eal ER S 5 WI 2 81 xl R9 Bestand Bewerken Beeld Invoegen Opmaak Extra Grafiek Yenster Help 28 x lose e 6Qar s Belo r5 i c Bru EessH szx e B 5 A Tekengebied Reeks1 Reeks2 20300 300 00 400 00 500 00 600 00 700 00 800 00 900 00 1000 00 1100 00 120 00 au OO Econ E RC RR MR RR RPM EREE RR 4 MI NGraphi AvaSoft Data Test sheet SESS A Gereed INUM 4 5 6 Limitations and Optimization Notes As all scans are stored in memory it is very easy to overflow internal memory with scans in Export mode Therefore a limit to the number of scans to store in Excel has been built in Before a new sheet is added the amount of memory Excel uses is determined If this number is larger than half the physical memory installed in the machine output to Excel will stop On a 128 MB machine this corresponds w
97. ched to getSpec USB2 by connecting an IC DB26 2 interface cable between the high density 26 pole Sub D connectors at the getSpec USB2 and the 15 pole DB connector of the getLight XE If used with a multi channel system make sure that the getLight XE is connected to the master sync spectrometer only the number of flashes per scan set for the master synch spectrometer will determine flash rate To disable the strobe simply enter 0 under the NrOfFlashes info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 31 more than spectroscopy Gel Spec com 3 27 Setup Menu 1 kHz Enable USB1 platform only CESS ID Spectrometer Nr fFlashes Pin 2 of the high density 15 pole Sub D connector at the getSpec 2048 can be used to generate an 1 kHz signal This signal can be used to control an getLight LED light sony UA o source in pulsed mode 3 2 8 Setup Menu PWM getLight LED control USB2 A Cancel platform The getLight LED needs to be attached to getSpec USB2 by connecting an IC DB26 2 interface cable between the high density 26 pole Sub D connectors at the getSpec USB2 and the 15 pole DB connector of the getLight LED DO1 pin1 1 The frequency can be set between 500 Hz and 300 kHz the duty cycle between 0 and 100 9 If use
98. cn Flenare 021200052 H VIS LNGOHSPS0 dh measurements mes a 00 Pr According to what has 400 600 500 1 000 been selected in the bou Save settings irradiance chart r p F iit parse ate paral in nita Ed settings dialog it will display the Eare iradence spectrum Yes locus for the 2 or 10 degrees standard observer The coordinates will be displayed in will be drawn from the midpoint the measured x y to the edge of the locus which represents the dominant wavelength measured x y the diagram and a line x y 1 3 through The scope data graph displays the raw A D counts that are received from the spectrometer This graph can be used to determine if a good integration time has been set If the integration time is too low the radiometric spectrum will be noisy if the integration time is set too high the spectrometer detector can be saturated in which case a saturated label will appear in the Scope Data Graph The radiometric graph displays the spectrum for the selected radiometric parameter Just like with selecting the radiometric parameter in the radiometry box the available parameters depend on the settings in the irradiance chart settings dialog In the figure at the right an LED is measured inside an integrating sphere in which case only the Radiant Flux emitted power in uWatt and the emitted energy during one integration cycle in uJoule can be selected See also the radiometric table in section 4 4 1 Background
99. cripts are plain ASCII files named Fx script txt where x is the history channel number from 1 to 8 A starter script has the following contents Function F2 value F2 0 End Function You can elaborate on this function as long as there is a value assigned to F2 in the end In this case F2 will be assigned the value O You can refer to the other history channels with the predefined variables F1 to F8 If you want to use data from the spectrometer you will need to assign other history channels to e g wavelength values or integrals The following script defines the function value for F3 as the quotient of F1 and F2 Function F3 value F3 F1 F2 End Function Please do not refer to the function value for the script itself You can also not refer to other functions if these are themselves assigned to a script This will result in a scripting run time error If you want to use code from another script please copy the necessary lines from that script into the one your working with info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 57 Imore than spectroscopy gel Spec om Please understand that a script is executed by an interpreter on a line by line basis If you make a syntax error in your script it will usually only show at the moment the line w
100. d corresponding concentration will be visualized as a new point in the graph Now move the cursor in the table to the second row setup the experiment for measuring the sample of known concentration for point 2 30 mmol l in the example above and click the Read Absorbance for point 1 button to import the measured absorbance into the left column in the table for point 2 Repeat these steps for the other samples of known concentration For a linear calibration line at least two samples are needed but the more points added the more reliable the calibration line will be After the samples have been measured click the Calculate New Calibration Line button As a result a red line will be fit through the measured data and you will be asked to save the calibration or not The figure at the right shows that the calibration line fits very well through the measured points so click Yes to confirm and to save the calibration If during the measurements an incorrect calibration point was measured for example because of mixing up the samples or measuring the concentration at the wrong row this will become clear from the graph You can then select either directly the No button or select the Yes button and modify the calibration line later on After clicking Yes a dialog is shown in which the name of the calibration file can be entered Type in the name you will use for this he calibration line MyFirst in the Desktop figure at
101. d and loaded under a user defined filename Click the Enable Custom Standard radio button and then OK in the Custom Reflectance Settings dialog to use the customized reference data in getSoft The status of this option is shown in the button bar by the addition of a green border to the white button If the border is absent 100 reflectance will be assumed for the reference tile If the border is present like in the figure above customized reflectance values will be used for all calculations 3 2 9 10 Setup Menu Options Suppress Save Comments This option if preceded by a checkmark disables the appearance of the comments dialog box if an experiment is saved as described in section 3 1 7 File Menu Save Experiment By default this option is OFF After clicking the menu option it will be enabled preceded by a checkmark 3 2 9 11 Setup Menu Options View Reflectance instead of Transmittance Although mathematically equivalent the experimental setup for a measurement in reflectance or transmittance mode is different and therefore the possibility to select the name Reflectance instead of Transmittance is implemented By default the option is not preceded by a checkmark meaning the program will use Transmittance mode By clicking the menu option a checkmark will appear and the program will use the name Reflectance instead of Transmittance The effect of the change is twofold o In Reflectance T
102. d if the distance from source to diffuser surface has been specified The radiant flux uWatts emitted and emitted energy uJoule e can be calculated if the geometry of the lamp in steradians is specified Distance and Geometry can be entered if the parameter is selected for which these data entries are relevant If one of the peak parameters is selected the wavelength range needs to be entered over which the highest peak will be calculated Moreover the radiometric parameter should be specified to determine a peak parameter Photometric parameters do not need a wavelength range because for these parameters the range is always from 380 to 780 nm However since emitted Lumens and Candela are the photometric equivalent of radiant flux and radiant intensity the geometry for emitted Lumens and distance for emitted Lumens and Candela needs to be specified if the source is not measured inside an integrating sphere If a source is measured inside an integrating sphere the radiometric parameters that can be measured are limited to radiant flux uWatts emitted and emitted energy uJoule e and the photometric parameter that can be measured in this hardware setup is emitted Lumen o Function display settings The parameters that have been set in the functions can be displayed graphically against time The amount of time that will be displayed at the x axis can be set manually under the x axis setting The y axis can be set to a fixed range or to
103. d the main window and menu is activated The yellow Pause button can be used to stop the time measurements temporarily After clicking the Pause button data acquisition stops and the text on the button changes to a green Start If the Start button is clicked data acquisition is activated again and the text changes back to the yellow Pause again The Save Reference and Save Dark buttons have the same functionality as in the main window The Info button shows the dialog in which all parameters can be viewed not edited while the measurement is running By clicking the Print button the graphics that are displayed can be printed during the run First the printer specific dialog shows up in which the print options can be set For example if only one graph of the four in the figure above need to be printed this graph can be selected by the page s radio button All graphics are printed full size on a different page info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 75 more than spectroscopy Gel spec com At the right top of each graph a color indicator panel shows the color of the sample being measured If the function output is a color difference value like dE in the figure above then a second color indicator panel is
104. d with a multi channel system all channels can have their own independent PWM setting for both frequency and duty cycle To disable the PWM output simply enter 0 under the Duty Cycle E frmSetPWM BERE Spectrometer Duty Cycle 0 100 2 Freg 500 300000 Hz 3 2 9 Setup Menu Options aE 5 14000 TAOS 14 2t 1000 Check on Saturation Full width Half Max Integrals XX Cancel Autasave Spectra Periodically Correct For Drift Automatic Save Dark bv TTL shutter External Trigger Setting Autoconfigure Integrationtime Use Custom Reflectance Reference Suppress Save Comments Dialog View Reflectance instead of Transmittance All options described in this section are standard included in getSoft FULL version In getSoft BASIC these features are not available 3 2 9 1 Setup Menu Options Check on Saturation The 14 bit A D converter in the getSpec results in raw Scope pixel values between 0 and 16383 counts If the value of 16383 counts is measured at one or more pixels then these pixels are called to be saturated or overexposed Since saturated pixels can disturb the measurement results a lot of attention has been given in getSoft and the driver package as161 dll AS 5216 DLL to detect saturation and to notify the user if a measurement contains saturated pixels This notification is done in such a way that the user can always decide to ignore the saturation for example if the saturation happens at pixels that
105. dd the pct extension and click Open To open an existing file simply double click on the filename or select it and click the Open button The file example pct can be found in getSoft s main directory default oo mE CAgetSpec60 and contains a few Bestandstypen Reference Color T able Annuleren measured reference colors To select a reference color from the database file click the select button below of the DB button The contents of the database file will be displayed as shown in the figure below To select one of the products from the list click on one of the numbers 1 11 in the first gray column To delete a product from the list select it and click the delete key on the keyboard To search for a certain productlD or number click the Find button To sort the records by ProductlD L REF a REF or b REF click on the E Reference Product Color Table DE x corresponding column header A yellow marked 1 will be shown in the column e header By clicking again at the header which is already marked the sorting direction will be converted PreductID L REF aREF b REF Color AR 1 19 280 2720 31 840 o By clicking the Cancel button the am 00 300 ANN selected product will not be moved to i20 aroo 4000 NENNEN the current reference color in the 42 290 44430 8990 pz LABChart settings dialog and changes 59740 22810 34600 IR e g deleting a prod
106. difference between the first two columns You can edit the values of the second column by selecting them with the mouse select the polynomial order In most cases a third order polynomial will show an excellent fit see figure above The include 2 order peaks option can be enabled if second or third order peaks should be added to the list of available literature peaks e g 507 30 nm as second order peak for 253 65 In most spectrometers the second order effects are eliminated by filters or coatings but if these options have not been added to the spectrometer and second order peaks are available then these can be included in the calibration The central column cells will be marked green if a second order peak is found and yellow if a third order peak is found Press the Calculate button If your calibration is successful you will be asked to confirm new Confirm Intercept 11 Points Used Calculated Calibration Coefficients are 3 901 35469030058E 002 Coefficient 1 2 88869698249683E D01 Coefficient 2 8 12149333338425E 006 Coefficient 3 1 89686889053452E 009 Coefficient 4 0 00000000000000E 000 St Deviation 0 0857 Range is From 350 nm to 888 nm Do you want bo SAVE these Coefficients Cancel info getSpec com 49 0 351 871 8423 T l 449 0 351 871 8465 Fax 33 0 4 72 80 82 02 Tel 33 0 4 72 80 82 43 Fax 449 0 231 974 2285 449 0 231 974 2
107. dvantage of the Correct For Drift By Spectrometer Channel is that the data for reference and sample are measured by using two optical benches which may react differently on temperature changes If the drift is mainly caused by the light source such as a difference in flash intensity of the XE 2000 this method of correction is recommended The advantage of the Correct For Drift By FOS 2 INLINE UV VIS is that it will also correct for drift caused by temperature changes of the optical bench Disadvantage is that the correction is sequentially so not every scan is immediately corrected Moreover there needs to be time available to switch channels info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 39 more than spectroscopy Gel spec com Correct for Drift by FOS 2 INLINE UV VIS The FOS 2 INLINE UV VIS is a 2 channel fiber optic switch Switching between the optical channels can be done manually or by a TTL signal see operating manual or FOS 2 INLINE UV VIS hardware manual By connecting an IC DB15 2 interface cable between the getSpec 2048 and FOS 2 or an IC DB15 FOS2 2 if a shutter needs to be controlled as well from the getSpec 2048 the spectrometer can control the position of the switch In getSoft the bottom channel has been defined as reference ch
108. e radio button needs to be clicked followed by the OK button However the spectrometer firmware will not return to normal acquisition mode before it receives the TTL pulse at pin 4 getSpec 2048FT and external hardware trigger The getSpec 2048FT has been developed for the LIBS Laser Induced Breakdown opectroscopy applications but can of course also be used in applications that need a fast response to an external trigger signal such as when measuring products at a conveying belt If the getSpec 2048FT scanning is controlled by an external hardware trigger signal at pin 4 of the DB15 connector it will carry out the following events after receiving this trigger signal 1 First it will respond by putting a TTL output signal at pin 2 of the DB15 connector This TTL output can be used to fire the laser in the LIBS application 2 heintegration time cycle starts an user defined delay after the TTL output to the laser has been given The minimum period for this delay is 42 nanoseconds in which case the laser pulse itself will be measured because the integration time will start 42 ns before the TTL out at DO2 The setting of the delay period can be increased with 42 nanoseconds steps The maximum delay is 2730583 ns A frequently used delay setting in LIBS applications is 1000 ns There is no jitter on the delay period which makes to getSpec 2048FT not only suitable for simple classification but also for precise quantitative analyt
109. e Experiment Note that the dialog allows you to select different folders or drives to save the experiments to as well as creating a new folder name for the new experiment For USB1 platform the default folder in which data is saved is called data lt serialnumber gt in which serialnumber refers to the serial number of the getSpec 2048 spectrometer that is being used for USB2 platform the default directory is data After closing the dialog box by clicking the Save button the new experiment name followed by its sequence number is displayed in the lower left of the status bar By clicking the Cancel button the old experiment name will be restored info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 19 more than spectroscopy Gell spec com 3 1 2 File Menu Load Dark With this option dark data can be loaded that have been saved before If getSoft is in static mode the dark data that will be loaded are shown on the screen first 3 1 3 File Menu Load Reference With this option reference data can be loaded that have been saved before If getSoft is in Static mode the reference data that will be loaded are shown on the screen first 3 1 4 File Menu Load Experiment Load Experiment Name E E 3 xl Suchen in Sj data simulation
110. e Reference button A white line will mark the reference spectrum Then switch off the calibration lamp wait until the spectrum becomes flat near the bottom of the scale and click the black button to save a dark spectrum A black line will mark the dark spectrum Click the Save Intensity Calibration button A dialog opens in which the current settings in this intensity calibration are shown If the calibration has been performed with diffuser the intensity calibration data will be saved to an ASCII file with extension dfr with bare fiber this extension will be fbr The name of the intensity calibration file can be entered after clicking the Save As button With USB2 spectrometers the intensity calibration can also be saved to EEPROM by clicking the Save to EEPROM button Click the Close button to close the dialog owitch to the Irradiance Chart TAB to enter the hardware setup and select the colorimetric radiometric photometric and or peak parameters of interest see section 4 4 5 1 Irradiance Chart Settings Then click OK Measure the output parameters in the experiment If needed change the integration time such that the maximum in scope mode is around 14000 A D counts Block the light path to the spectrometer and save a dark spectrum If the ir radiance of the light to be measured needs to be displayed against time click the time measurement TAB in the settings dialog as described in section 4 4 6 Time Series Meas
111. e been calculated by using the calibration coefficients have been added for the standards into the right column of the table By comparing the predicted values with the actual concentration values you can get an impression about the quality of the calibration line The actual calibration line will be automatically loaded the next time getSoft is started By clicking the Load button other calibrations that were saved before can be loaded after which the loaded calibration line becomes the actual line 4 6 2 2 Modifying a calibration The actual calibration line can be modified by changing the fit order of the calibration line or by adding or deleting calibration points Fit order The default fit order is linear Using a second order parabolic curve makes it possible to compensate for non linearity in e g your dip probe To illustrate this the calibration from the previous section was loaded MyFirst cal and the fit order was changed from first order to second order After clicking the Calculate New Calibration Line button the new standard deviation showed a much better fit then the previous linear fit After saving this calibration line to MySecond cal the predicted c concentrations showed also a much better fit for the quadratic model However certainly with this few calibration points the second order gof option should be used with care as it will ECS happily draw a well fitting calibration curve also thr
112. e color parameters can be selected Functions for which the color parameter has been set to None will be disabled If one of the color difference parameters dL da db or dE is selected the reference color to which the measured color needs to be compared needs to be defined as well Therefore the reference color definition box figure below will be displayed if dL da db or dE is clicked Reference Colo Current Reference Color c DB File D test data example pct 56 28 aREF f 0 45 bFEF ri 66 LREF Select Select Current Reference Color from T able PraduetlD vellow Add Add Current Reference Color to T able For each function a different reference color can be selected A detailed description about the DB Select and Add buttons can be found in the previous section The color parameters that have been set in the functions can be displayed graphically against time The amount of time that will be displayed at the x axis can be set manually by clicking the Fixed radio button see figure below If set to Auto the time axis will be set to 1 minute The y axis can also be set to Fixed or to Auto The Auto option will set the y axis range to the minimum and maximum function values that are in the list of measured data points Function Display Settings Yr axis Function Value C Auto Fixed Seconds l hin 135 00 Display Last fi Minutes C Hours Mas 105
113. e the measured L a b values online to a database and use one of the products from the database as a reference color By comparing the measured L a b values to the stored database values color differences Ej ap L a or b can be measured as well Emissive color measurements can be done to measure the color of a light source e g LED s These measurements require an irradiance spectrum to calculate the color parameters x y and z With getSoft s Irradiance application it is possible to perform irradiance measurements with a lot higher accuracy than when using the relative irradiance mode which assumes a perfect blackbody light source with known color temperature For this reason the emissive color or color of light calculation is one of the features in the irradiance application info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 66 more than spectroscopy Gel spec com 4 3 1 Color of an Object Background The color of an object can be expressed by the CIE 1976 L a b color space L describes the lightness of the color A positive value of a describes the redness of the color a negative a the greenness Similarly yellowness or blueness is expressed by coordinate b which is positive for yellow and negative for blue The L a b valu
114. ed in real time For LED measurements the dominant wavelength and purity are interesting parameters which can be calculated as well The same experimental set up spectrometer with fiber optics and cosine corrector or integrating sphere is used to calculate the photometric and or radiometric parameters of the light to be measured The calculated output can be displayed and saved in three ways o Inthe main window the data can be displayed as spectral irradiance in uWatt cm nm versus wavelength o Themeasurement results can also be displayed in an irradiance chart figure above which shows the chromaticity diagram the irradiance spectrum the spectrum in scope mode and many parameters for colorimetry photometry radiometry and peak measurements Up to 10 different radiometric parameters and or wavelength ranges can be selected for which the output will be displayed info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 77 more than spectroscopy Gel spec com o In Time Measurement mode up to 8 functions can be displayed simultaneously against time For each function a different radiometric photometric color coordinate or peak output parameter and or wavelength range may be selected as well as a different spectrometer channel 4 4 1 Background Bef
115. ee ee ee ee eeeeeeeeeseseseeeeeeeeeeeeeeeeeeaeaes 55 4 1 2 History Application Start Measuring cccccceceeeeeececeeeeeeseeeeeeeeseseeeeaeseseeeenseseseeess 61 4 1 3 History Application Display Saved History Graph sesseeseeeseeeesseesse 63 4 2 Application Wavelength CallDratllOni uccci sunset atre ix eir eas ait aeo o Praesta 64 4 2 1 Wavelength Calibration Perform New Calilbration cccccccecseceeeeseeeeeeeeeaeeeeaeeeeaeees 65 4 2 2 Wavelength Calibration Restore Original Calibration c cceeceeeeeeeeeeeeeeeeeeeeeeeeees 66 4 3 Application Color Measurement cccccccececeececeeceeeceeeeeeeeeeeseeeesueeeeeeeeeseeaeseeaeseseesesgees 66 4 3 1 Color of an Obireet Background sux scsi suck rbaicttc cra tate aug re rk E slt bt n a a 67 4 3 2 G lor Measurement RENT u A 67 LEUR AB AA c 68 4 20 2 2 MAIS SEIS ara aident EM sae teeane E cap M AN LL I EM RAD DUUM E 72 4 4 Application Absolute Irradiance Measurement ssssseee 77 4 4 1 zie de fo10 qe een ent nee nee ne eee ee ene eee nee re ne ee ere 78 4 4 2 Ge ao f gaara ee T QE 85 Quick Start 1 Perform absolute irradiance measurements using a calibrated lamp 85 Quick Start 2 Perform absolute irradiance measurements by loading an intensity calibration 86 4 4 3 Load Intensity Calibration ssseessse
116. ef 2 Load St Dev Read bsorbance for point 1 Exit Calculate New Calibration Line r Select Variables Channel Master v Peak at 560 nm width fi 0 nm Conc Unit mmol A Start New Calibration Clear Data concentration en LL i Absorbance Fit Order of Calibration Line First Order Linear Default Calibration Points C Second Order Quadratic il a eee i Coef 1 Actual Calibration Filename Master E Coef 2 Load 70 St Dev Calibration Line Coefficients Actual Points Actual Line m New Points New Line m m m Read bsorbance for point 1 vl Exit Imore than spectroscopy Gel Spec com If the zero concentration reference sample is included like in the example at the right set up the experiment to measure this reference Use the keyboard or mouse to point out the row in the table for which sample the absorbance value will be measured When moving through the rows in the table the text in the button Read Absorbance for point x will be updated for the sample that will be measured In the example we start with measuring the absorbance of point 1 which is the reference sample Click the Read Absorbance for point 1 button to import the measured absorbance into the left column in the table If the reference is measured the value should be very close to 0 000 The measured absorbance an
117. els to wavelength points is not a linear function so before writing to the JCAMP format the 2048 for an getSpec 2048 data points are linearized first This option requires that graphic files were saved earlier by using the option File gt Save Experiment After choosing this option a window shows all files in the current measure mode For instance if the measure mode is scope so the extension of the earlier saved spectra is roh To select graphic files that were saved in another measure mode e g absorbance click z behind the Graph Mode and pick the desired measure mode To select graphic files from another folder or drive click El behind the current folder name If a graphic file is marked by a single mouse click on the filename the comment line for this file appears at the top of the graphical region in the main window Selecting multiple filenames can be realized by using the CTRL or SHIFT key in combination with the mouse If the CTRL key is pressed all the files that are clicked by the mouse will be selected for conversion If the SHIFT key is pressed all the files in between two clicked files will be selected for conversion Select the name of the file s to be converted to JCAMP and click the Open button To leave this dialog without converting files click the Cancel button The text files in JCAMP format require an extension DX If for example a file gts0001 trm and another file gts0001 a
118. emple dark 2 9 ref dark 9 As described in section 3 2 9 9 Setup Menu Options Use Custom Reflection Reference the reference white percentage of 100 can be customized The percentage of transmittance is mathematically equivalent to the percentage of reflectance and can also be used for reflectance experiments see also section 3 2 9 11 Setup Menu Options View Reflectance instead of Transmittance 3 3 4 View Menu Irradiance Mode If the Absolute Irradiance Measurements Module has been ordered with getSoft this option will show the absolute energy output in uWatt cm nm An elaborate description about the experimental setup in case of absolute irradiance can be found in section 4 4 Application Absolute Irradiance info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 50 more than spectroscopy Gel spec com If the absolute irradiance application is not available a light source of known color temperature is needed as a reference for example the getLight HAL with color temperatures of 2850 K at default jumper setting The relative radiance energy at wavelength is then calculated using the current sample the reference and the dark data sets S B lsample dark Where B is the computed component of the spectral distr
119. ensity is calculated from the measured irradiance by multiplication with the square of the distance between source and diffuser surface It is assumed that the source is a point source o lrradiance uWatt cm Irradiance is used to measure the power that is received by a surface Radiometric measurement can be done in different setups like with fiber optic cosine corrector or integrating sphere Both setups can be used to measure the irradiance spectrum received at the surface of the diffuser cosine corrector or integrating sphere sample port at a certain distance from a light source When measuring at a certain distance from the source the radiant intensity and flux can be calculated as described above When measuring a light source inside an integrating sphere the radiant flux can be measured but radiant intensity and irradiance parameters cannot be measured Radiometric parameters calculated from the power distribution The power distribution can be easily converted in an energy distribution by multiplying the power with the integration time The result is the amount of energy that has been emitted or received during one integration time cycle Another radiometric parameter that can be calculated from the irradiance spectrum is the number of photons that is received by a surface Since the number of photons per nanometer is a huge number even with very low light intensity the number of Avogadro is used to express the number of photons
120. enuator a neutral density filter or fibers with a smaller diameter may be used When not enough light reaches the spectrometer likewise a longer integration time should be entered When a good spectrum is displayed turn off the light source Now save the Dark data This is be done by File gt Save Dark from the menu or by clicking the black square on the left top of the screen with the mouse Always use Save Dark after the integration time has been changed Turn on the light source again Save the present spectrum as a reference by choosing File gt Save Reference from the menu or by clicking at the white square next to the black one Always use Save Reference after the integration time has been changed Now the transmittance reflectance T button or absorbance A button spectra can be obtained online To have a better look at the amplitude versus wavelength the cursor button can be clicked A vertical line is displayed in the graph If the mouse cursor is placed nearby this line the shape of the mouse cursor changes from an arrow to a drag shape If this shape is displayed the left mouse button can be used to drag keep left mouse button down the line with the mouse towards a new position Moving this line info getSpec com 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Do
121. es are derived from the CIE tristimulus values X Y and Z of the sample object and the standard illuminant tristimulus values X Y and Z The standard illuminant tristimulus values for X Yn and Z4 are constant and depend only on the type of standard illuminant that has been chosen The CIE tristimulus values X Y and Z of the color of an object are obtained by multiplying the relative power P of a standard illuminant the reflectance R or the transmittance of the object and the CIE standard observer functions x y and z 2 degrees or 10 degrees angle The integral of these products over all the wavelengths in the visible spectrum 380 to 780 nm with a 5 nm interval gives the tristimulus values The chromaticity coordinates x y and z are obtained by taking the ratios of the tristimulus values X Y and Z to their sum X Y Z x 4 2 PN SM 2Y Z x y2 7 X Y Z Another well known way to present the color parameters a and b is by their hue angle h and Chroma C Hue angle is measured in degrees starting with h 0 in the red direction a and increasing counterclockwise h arctan a Chroma is defined as the length of the line from the point a b 0 to the sample point C 4a 2b To describe color differences a well known parameter is EL ab which is defined as E 4 4 L9 2a py In which L a and b represent the difference in L a b values between
122. essed all the files that are clicked by the mouse will be selected for conversion If the SHIFT key is pressed all the files in between two clicked files will be selected for conversion Select the name of the file s to be converted to ASCII and click the Open button To leave this dialog without converting files click the Cancel button The extension of the text files depends on the extension of the binary graphic file as shown below Extension binary file Extension text file info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 24 more than spectroscopy Gel spec com ROH TRT ABS TAT TRM TIT IRR TIT All text files start with a header with information for the graphic file that has been converted The header shows the comment line the integration time the number of scans that has been averaged the number of pixels used for smoothing the serial number of the spectrometer that was used to save the data O O O O The data in a TRT file is given in two columns The first column gives the wavelength in nanometers the second one the scope data The data in the TAT TTT files is presented in five columns The first column gives the wavelength in nanometers The second to fourth column give respectively the dark reference and scope d
123. every scan The name of the file to which the data will be saved can be changed after clicking the Change Output File button Last option in the Save Function Output box is to enable or disable the possibility to create a backup file during the measurements If this option is enabled getSoft will create a backup file with the same filename but with the extension bak also in ASCII This backup file is updated every scan and can be used in case the filename that has been selected has failed to save the data for instance because of a power failure during the measurements Save Setting Load Settings buttons GetSoft automatically saves all parameters color parameters save options to the file cootime ini and restores these parameters the next time getSoft is started With the Save Settings and Load Settings buttons it is also possible to save these parameter settings to and load from a file with the extension coo With this feature it is possible to save for each experiment a different coo file so it can be loaded a next time the same settings are required The left figure below shows the dialog in which the name of the file can be entered after clicking the Save Settings button The right figure below which is displayed after clicking the Load Settings button illustrates how to select an earlier saved coo file info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fa
124. f this option is activated a dialog is shown in which the reference channel and one or more depending on the number of spectrometer channels that are available channels to be corrected can be selected X Cancel After selecting the right setup and clicking the OK button getSoft shows the right information Turn on your light source choose the right integration time and save reference Then turn off the light source and save dark After clicking OK getSoft enables the spectrometer channels that are relevant in the correct for drift application After saving the reference and info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 42 more than spectroscopy Gel spec com dark files a message dialog shows that the data will be corrected for drift The menu option will be preceded by a checkmark To disable the correct for drift option the menu option if preceded by a checkmark needs to be clicked 3 2 9 6 Setup Menu Options Automatic Save Dark by TTL shutter To use the automatic save dark option an interface cable needs to be connected from the spectrometer to the light source with shutter getLight HAL S getLight DHc getLight D H S DUV The interface cable between TTL shutter and spectrometer is for the USB1 platform a 15 to 15
125. factory calibration The Process data only when in following wavelength range option can be used to transfer only a limited number of pixels from the spectrometer to the PC This can significantly speed up the transfer time e g for the getSpec 2048 from 30 ms at full wavelength down to 14 ms for a small selection of 10 pixels A second advantage is data reduction because only the spectral data will be saved at the pixels for which the wavelength is in the specified wavelength range 3 2 3 Setup Menu Smoothing and Spline The cubic spline interpolation algorithm can be applied to get a better estimation for the spectral data between the pixels on the detector array Smoothing is a procedure which averages the spectral data over a number of pixels on the detector array For example if the smoothing parameter Is set to 2 the spectral data for all pixels x on the detector array will be averaged with their neighbor pixels Xn 2 Xn 1 Xn 1 and Xn42 Cubic Spline Interpolation In the figure at the right the effect of spline interpolation is illustrated The Master data shows the AD counts for 4 pixels connected by a straight line linear interpolation The Slave1 data is for these 4 pixels exactly the same as for the Master data but this time the cubic spline interpolation algorithm has been applied resulting in data which is smooth in the first derivative and continuous in the second derivative The spline interpolation can be u
126. fter disabling External Trigger mode and providing one more trigger an edit box will show up with the current delay setting see figure below The delay setting can be changed in this edit box 42 to 2730583 nanoseconds After changing the delay you can enable the external hardware trigger by clicking the Trigger checkbox ET GetSofb ZO Full 2005 GetSpec ET 84 x File Setup View Application Help soi Wass SATI DAX 6E E Pr Integration time ms n Average f Wavelength nm 656 87 m Trigger Delay 1000 ns Il m Trigger Checkbox Note that the resolution of the delay in nanoseconds is steps of 41 67 ns with an offset of 41 67 ns So if you enter e g a delay of 800 ns the software will calculate the closest step 41 67 20 41 67 792 ns For the spectra that are saved in external hardware trigger mode a comment file will be created automatically in which the date time and delay are shown This may help to compare the spectra that were recorded with different delay settings to determine an optimal info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 46 Imore than spectroscopy Gel Spec com delay File gt Display Saved Graph The optimal delay period depends on the material a
127. function is defined to display a peak versus time the following parameters can be set The Spectrometer Channel The default is Master but in case more channels are available this can be changed to Slave1 Slave2 up to Slave7 The From and To edit boxes may be changed to specify the wavelength range in nanometer which will be evaluated in the peak search Finally a selection can be made to define the Peak Output Wavelength or Intensity If both wavelength and intensity need to be followed against time one of the functions F1 F8 can be used to follow the peak wavelength and another function to follow the peak info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 59 more than spectroscopy Gel spec com intensity To visualize the peak changes a third function can be set to View Spectrum with e g the wavelength range set to 455 457 as well and the nr of peaks to display set to 10 In the figure above the resulting output will be the wavelength of the highest peak between 455 and 457 nanometer If the intensity button had been selected the function output would have been the intensity of the highest peak between 455 and 457 nanometer Function Display Setting Function Display Settings Yr axis Function Value C Auto
128. g purpose After clicking the ET OK button the integral values are Zz CT Mo MIU i g l Ho i Maste Fmaj tofin nm 10000 given as shown in the figure below ILI M e From fiat oia em fa To disable the integral calculation the menu option which is marked as X Corel long as the Integrals are enabled needs to be reselected To measure the integral versus time up to 8 different functions can be entered in the History AED EE dvenbee Ss abai ail sj NM MeSATIDEHe24Lx65M miae dale o e ioo Vierte md ana e ma Fi aoe Ee EH Aa JT Sethe D ee oe eed pe LIO eterna application info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 37 more than spectroscopy gel Spec com 3 2 9 4 Setup Menu Options Autosave Spectra Periodically With this option complete spectra will be saved automatically in time The following parameters can be set o Time delay before first scan needs to be entered in seconds After clicking the OK button getSoft waits for this number of seconds before the Save As Fast As Possible no screen updates first scan is saved Time delay before first scan Save Parameters seconds be entered in seconds This defines Number of scans to save the time between saving two subsequent spectra If th
129. getSoft 7 software all connected spectrometers will be recognized automatically and the serial numbers will appear as labels on the right hand of the screen After clicking the Start button all connected spectrometers will be displayed in the main window After clicking the OK button the main window is displayed Refer to section 2 for a description about the main window components A Quick Start can be found in section 1 if you want to start measuring immediately Detailed information about the menu options is found in section 3 Depending on the getSoft version Basic or Full and the extra add on modules that were ordered for your spectrometer up to six applications are available in getSoft which are described in sections 4 1 to 4 6 info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 9 more than spectroscopy Gel spec com Tel Fax History standard in getSoft FULL Wavelength Calibration standard in getSoft FULL Color Measurement add on module Irradiance Measurement add on module Excel Output add on module Chemometry add on module OD OU a oa wi Quick Start Measuring and Saving a Spectrum After starting getSoft the green Start button needs to be clicked to start measuring Connect a fiber or p
130. getSoft and the spectrometer waits for a next pulse The external hardware trigger is useful to observe short pulse events i e laser pulses To measure a laser pulse the getSpec 2048FT includes an option to send out a TTL pulse at pin DO2 to fire the laser just before the integration time cycle starts delay 42 ns By setting the delay between the TTL out at DO2 and the start of the integration time to a positive value the getspec 2048FT can be used in LIBS application Laser induced breakdown spectroscopy where the laser pulse itself should not be measured but the plasma emission e g 1 s after firing the laser For details about the delay settings for the getSpec 2048FT in external hardware trigger mode see below If the white Automatic Save On Trigger box is marked each spectrum that will be recorded in external hardware trigger mode will be saved automatically Make sure that before the External Hardware Trigger is selected and confirmed by the clicking the OK button a 5V TTL pulse can be set on pin 4 To leave the external trigger mode the Setup gt Options gt External Trigger Setting menu option needs to be selected info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 44 more than spectroscopy Gel spec com and the Non
131. gration time such that the maximum count over the wavelength range is around 14000 counts When at minimum integration the signal is still too high fibers with a smaller diameter can be used When not enough light reaches the spectrometer likewise a longer integration time should be entered When a good spectrum is displayed turn off the light source Now save the dark data This is done by File gt Save gt Dark from the menu or by clicking the Save Dark button Turn on the reference light source of known color temperature 2850 K for getLight HAL with default jumper setting and set up the fiber end that is not connected to the spectrometer so that a good spectrum is displayed on the screen Note that the integration time or fiber type may not be changed while measuring the reference data If there is too much light adjust the focusing of the light source so less light is coupled into the fiber As long as only light from the reference light source and no ambient light is coupled into the fiber this will not influence the spectral distribution only info getSpec com 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 51 more than spectroscopy Gel spec com its height Try to set up the fiber such that the maximum count over the wavelength range is around 14000 coun
132. gration time during the sample measurements e g 2 ms a factor needs to be added to the equation to compensate for this In the example the factor is 100 2 50 Calculating the intensity in uWatt cm from the measured sample spectrum in A D Counts can therefore be done by the following equation d sample dark 3 I Caldata factor refcal darkcal 9 From the irradiance spectrum in uWatt cm a lot of light output parameters can be calculated colorimetric photometric and radiometric Below short background information is given about the colorimetric radiometric and photometric parameters We also describe the definition of the peak parameters that can be measured as well info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 78 m ore than spectroscopy Gel spec com Colorimetry The color of light can be expressed by the chromaticity coordinates x y and z These chromaticity coordinates are obtained by taking the ratios of the tristimulus values X Y and Z to their sum X Y Z x 30 D NN x y27 2Y Z 2Y Z The tristimulus values X Y and Z are computed by X ae aay y Y k I y Zee qe where K constant 1 y 0 00934 Spectral irradiance at wavelength X Y2 Z9
133. h dot dot dash dot To enable this option click the menu option and a checkmark appears in front of it 3 1 10 File Menu Display Saved Graph This option requires that Select one or more graphic files to open E A xl graphic files were saved Look in i date 020900681 c e amp em earlier by using the option File Save Experiment After choosing this option a window shows all files in the current measure mode In the example at the right the measure mode is scope so the extension of the earlier saved spectra is ROH To select graphic files that were saved in another measure mode e g I Obeceltpe iBaph Scope Mode bored absorbance click zl behind the Graph Mode and pick the desired measure mode To select graphic files from another folder or drive click El behind the current folder name If a graphic file is marked by a single mouse click on the filename the comment line for this file appears at the top of the graphical region in the main window Selecting multiple filenames can be realized by using the CTRL or SHIFT key in combination with the mouse If the CTRL key is pressed all the files that are clicked by the mouse will be selected for displaying If the SHIFT key is pressed all the files in between two clicked files will be selected for displaying Select the name of the file s to be displayed and click the Open button To leave this dialog without displaying graphic
134. ibution of the blackbody radiant emittance at user selected temperature in degrees Kelvin divided by the current reference data at wavelength How to take relative irradiance measurements with getSoft 1 2 3 Tel Fax otart the getSoft software and click the Start button in the main window Connect a fiber to the spectrometer input port Adjust the smoothing parameters in the setup menu to optimize smoothing for the used fiber slit diameter oet up the experiment so that the other end of the fiber points at the sample of light to be determined use a fixture for the best results Usually some sort of spectrum can be seen on the screen but it is possible that too much or too few light reaches the spectrometer at the present data collection settings Too much light means that over a certain wavelength range the signal is overloaded shown as a straight line at any arbitrary height even near zero This can usually be solved by a shorter integration time The integration time can be changed in the main window in the white box below the Start Stop button If getSoft is collecting data the Start Stop button shows a red Stop and the integration time box is gray indicating that it cannot be changed After clicking the Stop button the data acquisition stops and the integration time can be changed The result of the changed integration time can be viewed after clicking the green Start button Try to adjust the inte
135. ical applications The software programmable delay between the output at DO2 to fire the laser and the start of the integration time is used to avoid the measurement of the first period directly after the laser pulse in which the plasma emission shows a high intensity but without the useful information The figure below shows an example of the plasma emission intensity after the laser pulse The integration time for the getSpec 2048FT can be set from 2 ms to 60000 ms In LIBS applications the minimum integration time of 2 ms is used Laserpulse 5 ns s Vi T Plasma emission to be analyzed spectroscopically 50 100 us Programmable delay 42 ns to 2730583 ns in 42 ns steps jitter 0 ns This portion of the plasma emission is not included in the integration time info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 45 more than spectroscopy To set the delay in getSoft select the external hardware trigger option in the External Trigger Setting dialog as shown in the figure Enter the delay in nanoseconds between the output that will be given at pin 2 to fire the laser and the start of the integration time cycle If you want the spectra to be saved C Perform scans continuously save first available spectrum if TTL signal is high pin
136. ile Annuleren o Reference Color Reference Colo c DB File D Mtest data example pct Current Heference Color Select Select Current Reference Color fram T able LREF 82 24 aREF r5 BREF 72 13 Add Add Current Reference Color to T able ucro arene eee dere ProductiD 562 nm The reference color can be set to compare the L a and b color parameters during the online measurements with the LREF aREF and bREF of the reference color The color info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 69 more than spectroscopy Gell Spec com differences can be shown during the measurements by dL 2 L LREF da a aREF db b bREF and or dE dL daf db The reference color can be entered manually under Current Reference Color in the figure above or it can be selected from a database file The database file is a list of reference colors that were saved before to this file all with a unique ProductlD By default the name of the database file is empty pct By clicking the DB button a different database file can be selected or a new file can be created Dpenen HE To start a new database file for saving Zoeken in E data ex reference colors enter a filename that does not already exists getSoft will a
137. info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 34 m ore than spectroscopy Gell Spec com Visualize saturated pixels areas in graph By enabling this option the spectrum in the main window will be displayed with a thicker line for these wavelength ranges where the saturation occurs In time series measurements the function output values that were calculated using saturated data are marked with a black dot Eh Fidh Rees ae Efil aerated cee bi ee itj xj m Bee SATI IRS ke W k me Bua SATI OM G Ah E HET san j mta sd nia rh rmm om or E St em EUR PUE imm ri aco DO prem Seco Bernd d i ye FEES imio beii ee Ea Be 0 ee ee eur me IL TX Be s Log saturated pixel areas to screen By enabling the second option a small window appears in which the spectrometer channel number 0 Master 1 Slavei etc and wavelength range for the saturated pixels are given Log saturated pixel areas to file on Save Measurement The third option can be used to create a file to which the saturated wavelength ranges will be written when a measurement is saved in getSoft When saving spectra in the main window Save button or menu option File gt Save gt Experiment the name of the logfile becomes equal to the experimentname bu
138. is number is set to zero getSoft will save the spectra as fast as possible 10 o Time delay between scans needs to Time delay between scans 60 SECUS 5 x Cancel o Number of scans to save the number of spectra that needs to be saved can be entered On top of the parameters that appears at the right a checkbox shows Save As Fast As Possible no screen updates If the white checkbox in front of this text line is marked the Automatic Save option will always save the number of scans that have been entered as fast as possible To do so the time delay between scans is automatically changed to 0 milliseconds at the moment the checkbox is clicked Is this option selected the spectra will be saved 5 to 10 times faster compared to if this option is not selected This speed increase has been achieved by mainly two reasons 1 During the saving of the number of scans that has been entered the screen update which is very time consuming is not activated Instead the main window is temporarily minimized and the number of scans to save is counted down in a new dialog 2 No comment files will be generated Other factors that have a positive effect on the speed of the data acquisition and which can be set in getSoft are o Smoothing parameters o Integration time o Averaging o Number of pixels to transfer section 3 2 2 Furthermore it is recommended to keep the number of files in your experiment directory small
139. ith 7 8 complete sheets of 256 columns and 2000 rows roughly 2000 full scans Limiting the wavelength range will extend your range of scans accordingly Of course adding more RAM to your machine will also help raise this limit We have however seen limitations in the amount of memory that Excel can use It looks like there is an internal limit that is hit before internal memory runs out In a machine with 512 MB the limit corresponds with about 16 sheets of complete 2000 pixel spectra After this Excel issues an Out of Memory message and is no longer functional E g saving your data to disk is not possible anymore This is only an issue on machines with large amounts of memory 256 MB 512 MB For greatest speed it is important to give the focus to Excel If you set the focus to getSoft Windows will dramatically lower Excel s priority You can verify this by looking at the Filling Cells progress bar in the lower left corner of your worksheet info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 106 more than spectroscopy Gel spec com 4 6 Chemometry getSoft Chem was developed to enable on line concentration measurements with a spectrometer system According to Lambert Beer s law there is a linear relationship between absorbance and con
140. ith the error is executed as a run time error Nothing will stop you for instance from entering the following script for function 3 Fu nction F3 val ue Script Error E x F3 F1 0 e End Function 11 Microsoft VBScript runtime error Division hy zern at line z char l near Running this script will result in the run time error at the right If you refer to channels that are undefined Function Type None they will be handled as uninitialized variables by VBScript with value 0 This is the case for all uninitialized variables you might use in your scripts Finally a small example to monitor the integral of a peak between 522 nm and 550 nm in which an offset is subtracted The offset area is the area below of the straight line between the output at 522 nm and the output at 550 nm F1 is defined as a user defined input function m 522 F2 is defined as a user defined input function m 550 F3 is defined as the zero based integral between 522 nm and 550 nm The script for function F4 can then be written as Function F4 value If F1 gt F2 Then offset 28 F2 0 5 F1 F2 Else offset 28 F1 0 5 F2 F1 End If F4 F3 offset End Function Function Definition View Spectrum Function Definition Spectrometer Channel Wavelength range nm Master From 400 0 To 500 0 Mr af peaks to display li T The View Spectrum function does not display function output against time
141. ittance 49 3 3 M MU UD M 50 3 3 1 View Menu Scope Mode RN m TM 50 3 3 2 View Menu Absorbance Mode cccceccececeeeeeecececeeeeeeseeeeeeceseceeaeeesessegeeeseseeeneees 50 3 3 3 View Menu Transmittance Reflectance MOde cccccccececeeeeeeeeeeeeeeeeeeeeaeeeeeeesenenees 50 3 3 4 View Menu Irradiance Mode cccccececcececeeeeeececeeeeeeeeseeeeeeeesecseeeeeseeeegesesenseetgees 50 3 3 5 View Menu Channel only for USB1 platform cc ccccececeeeeeeceeeeeeaeeeseeeeeeeeeeeeeaees 52 3 3 6 View Menu Change Graph Scale cccccccececceceeeeeeeeeceeeeeseeeeeeeaeseeeeeeeseaeeeeaeeeeaeees 52 3 3 7 View Menu Graphic ReSet cccccecececeeeececeeeeeeseseeeeeeeeseceeeeeeseceeaeeesessegnseseneeeteees 52 3 3 8 View Menu AutoScale y axis is ccsccwncisisenadistacwucaiesdaeanaeaiicnnchdidwneniedbiencaescenmnenmitaabdicames 52 3 3 9 View Menu Goto Preset Scale iie eee serrano rae eh EY SR ksara raai 53 3 3 10 View Menu Grid Enable sssssssssssssssssesen nnnm emm serene nnns 53 3 3 11 View Menu Progress Bar Enable cccccececeeseceeeeeeeeeeeeeeeeeeeeeeneeeeeseseeeeaeseseeeeeees 53 4 APPLICATIONS 55 4 1 Application History Channel FUNCTIONS isicinscinbian wacwounn wince aceusauibinen wan rack cute vd i ci P ptu a ou 55 4 1 1 History Application Function Entry cccccceceeeeeee
142. l Save Setings Load Setings With time series color measurements up to 8 color parameters or color differences can be followed simultaneously in a graph against time The time series settings are defined in the dialog above which is shown after clicking the Time Series TAB page The functions F1 to F8 can be selected by clicking the corresponding TAB sheet at the top of this dialog Furthermore a number of general function independent parameters can be entered below of the function TAB sheets The following features can be set separately for all functions F1 to F8 O Tel Fax Spectrometer Channel If the spectrometer system contains one or more slave spectrometer channels the spectrometer channel on which the color will be measured can be selected from the drop down box at the upper left corner showing by default Master Make sure white reference and dark spectra have been saved before for the selected spectrometer channel illuminant The CIE Standard sources A B or C or one of the illuminants D50 D55 D65 or D75 can be selected by clicking one of the radio buttons The default is D65 which is the most widely used illuminant It represents the power distribution of average daylight with a correlated color temperature of 6500 K The D50 D55 and D75 have correlated color temperatures of 5000 K 5500 K and 7500 K The CIE otandard sources A B or C represent respectively Incandescent light 2854 K blackbody Simula
143. le point S divided by that from the midpoint E to the spectrum locus DW Purity ee E DW The method described above is used for all colors with a Dominant Wavelength from 380 to 699 nanometer If the x y coordinates are in the triangle area encompassed by the 3 points E A and B then the Dominant Wavelength can not be calculated because the interception point through E and S with the spectrum locus between A and B does not correspond with a wavelength In that case the Complementary Dominant Wavelength CDW is used The line from E through S is extended backward in order to determine the Complementary Dominant Wavelength CDW CIE 1931 2 degrees standard observer Chromaticity Diagram Power emitted nWatt hal 0 of Ao2 03 of o5 os oF 500 550 B00 650 700 X E Wavelength nm Peak Measurements A typical spectral power distribution of a green LED is shown in the figure at the right A number of peak parameters can be calculated from this spectrum r Peak Measurements FWHM and Center Wavelength Evaluate highest peak The Full Width Half Maximum of a peak is the bandwidth in Eoo Eoo LL nanometers for which the intensity is higher than half of the at 31 521 mm maximum intensity of that peak The Center Wavelength is the eee e Toine wavelength halfway between the left and right wavelength where the a TTET intensity is half of the maximum intensity Intensity 2 3755 Centroid WL 570 79 nra Inten
144. lected 3 3 6 View Menu Change Graph Scale After selecting this option a dialog is shown in which the range for both x and y axis can be changed To switch to Min Max AXIS 600 0 630 0 the full scale the View gt Graphic Reset option or the mouse zoom out feature can be used By clicking the Save button in this dialog the settings for x axis and y Y Anis 48 04 24 3 axis will be saved to a file and can be restored in the y UK X Cancel future by selecting the menu option View gt Goto Preset scale or by clicking the Goto Preset Scale button in the button bar 3 3 View Menu Graphic Reset When selecting this option the graph will be reset to the default x and y axes 3 3 8 View Menu Autoscale y axis By using this option the graph will be rescaled on line A maximum signal will be shown at about 75 of the vertical scale info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 52 more than spectroscopy get S pec com 3 3 9 View Menu Goto Preset Scale By clicking this menu option the scale for x and y axis will be set to a range that has been set before The same result can be achieved by clicking the Goto Preset Scale button in the button bar 3 3 10 View Menu Grid Enable When
145. low info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 56 more than spectroscopy Gel spec com Operators 4 Functions log x natural log log10 x log base 10 exp x e to the power of x sqrt x square root Additionally the wavelength signal for master and slave channel s can be entered by m wavelength in nm master s1 wavelength in nm slave1 s2 wavelength in nm slave2 etc For example the function that needs to be entered to follow the intensity of the HG peak against time at the second slave channel is s2 253 65 Scripts A scripting feature has been added to allow you to use more complicated calculations with many more mathematical and logical functions Scripts will also allow you to combine the results of several history channels getSoft uses the Microsoft VBScript language that comes with your copy of Windows We have included a help file for VBScript for your reference You can reach it from the main menu by selecting Help gt VBScripts It lists among other things all available operators and functions When you edit a script either the previous script for the channel will be shown or a new starter script will be generated if there is no previous script GetSoft includes a simple script editor The s
146. lready a peak If only one of the neighbor pixel values is higher then the current pixel value the peak will be searched in the direction of this higher pixel If both neighbor pixels info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 17 Imore than spectroscopy gel Spec om have a higher value at the y axis than the current pixel the current pixel is in a valley The peak will in this case be searched in the direction of this neighbor pixel with the highest value 4 he cursor starts moving in the direction as determined under 3 until it reaches a pixel of which the value is not higher than the last one evaluated At this pixel the cursor stops By holding down the SHIFT key instead of the CTRL key the same procedure will be used to move to the closest valley If more than one spectrum is being displayed a dialog as shown at the right pops up in which the spectrum for which the peak finder needs to be activated can be selected out of all displayed spectra a Select Graph Select the graph bor which Hh cursor reeds lo be mones Lo fhe reed peak then click OK Haste getSpec OG5 RIH getSpec 0035 ROH info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49
147. meters that need to be entered in the function definition box depend on the function type that has been chosen Integral User Defined View Spectrum or Peak Function Definition Integral Function Definition Spectrometer Channel Wavelength range nm Multiply with Master From 500 0 To 600 0 1 000 In case a function is defined to display the integral versus time the following parameters can be set opectrometer Channel The default is Master but in case more channels are available this can be changed to Slave1 Slave2 up to Slave7 The From and To edit boxes may be changed to specify the wavelength range in nanometer over which the integral needs to be calculated Finally a multiplication factor can be entered The value entered here is multiplied with the calculated integral Function Definition User Defined Function Detinitian Select LID Function Type f Input Function Script Edit Script Example log O m 456 n 765 321 0 234 z345 m 600 s1 600 If you want to display the output of a self defined function against time a function needs to be defined first You can either enter an user defined input function in the dialog box or assign a script to the history channel Input Functions A comprehensive example of an input function is given in the dialog but it illustrates only a few of the functions getSoft can handle A list of allowed operators and functions is given be
148. ncandescent light 2854 K blackbody Simulated noon sunlight and Simulated overcast sky daylight Note that there is no relation between the illuminant chosen here and the light source that is used for measuring the color of an object The selected illuminant is used in calculation of the color parameters These color parameters will be different if for example A will be selected instead of D65 just as the color of an object will look different in average daylight or in incandescent light 2854 K blackbody info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 68 more than spectroscopy Gel spec com o CIE Standard Observer The original CIE standard observer functions x y and Z were defined in 1931 and are known as the 2 degrees standard observer values The 2 degrees corresponds with the angle of vision that was used in the experiments to determine these standard observer values In 1964 the CIE recommended the use of different observer values for a higher correlation with visual perception for large samples These are known as the 1964 supplementary standard observer or 10 degrees observer values Both standards are still in use and in getSoft the CIE standard observer can be selected by clicking one of the radio buttons The default is 2 degrees o
149. nd the laser in the LIBS application External Trigger setting USB2 platform E External Trigger Setting E oj x Spectrometer Enable Nrorocans Save Spectrum Edge Triggered Level Triggered ae m Ser F d F X Cancel If the external trigger is enabled the spectrometer will wait until a TTL signal at pin 6 of the DB26 connector gets high and will then start the integration time The delay between the rising edge of the TTL pulse and the start of the integration time cycle depends on the spectrometer type as shown in the table below Spectrometer Type Minimum Delay s Maximum Delay s getSpec 102 USB2 wwennzo o NrOfScans If a number of scans is set to a value higher than 1 the spectrometer will accumulate multiple scans on every rising edge of the external TTL trigger This setting is overruled when Level Triggered is enabled Save Spectrum If this box is checked the scans that the spectrometer will accumulate will be saved to the hard disk If NrOfScans 1 the data saving will be done as fast as possible without screen updates Edge Triggered When this mode is selected one or multiple scans will be started at the rising edge of a TTL pulse at pin 6 of the DB26 connector The delay between the rising edge and the actual start of the integration time can be found in the table above info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel
150. nd the x axis is already scrolling will not be possible because in that case the x axis is updated with each new scan Clicking the Pause button to take a snapshot will solve this problem X Cancel Save HCF Load HCF Zoom in Select a region to be expanded to the full graph To select this region click the left mouse button in the white graphics region and drag it downwards and to the right After releasing the left mouse button both the x and y axis will be rescaled to the new values of the selected region Zoom out Drag with the left mouse button within the white graphics region but in stead of dragging the mouse downwards and to the right drag it into another direction After releasing the mouse button both the x and y axis will be reset to their default values Move X Y Dragging with the right mouse button results in moving the complete spectrum up or down and to the left or right 4 5 Application Excel Output getSoft XLS is an add on application that enables getSoft Full to output history channel data and or complete spectra to Microsoft Excel It uses OLE Automation which is a technology that enables getSoft to remotely control Excel opening sheets and copying data into cells On modern hardware we have been able to copy complete 2048 pixel spectra to Excel with as little as 50 msec overhead per scan 4 5 1 Select Source Data Besides enabling Excel output you will have to select your source dat
151. need to be printed this graph can be selected by the page s radio button All graphics are printed full size on a different page info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 99 more than spectroscopy Gel spec com If time measurement data have been OITUEEBMTAW 0 l Inx saved the resulting ASCII file can be m jr Fm dee des irs Er pee displayed graphically by selecting this file ron C Integral C User Defined e View Spectrum C Peak in the dialog that is shown after clicking NR the menu option Application gt History gt 6 Scope C Abobsce C Trananitance nance Display Saved History Graph After Functin Definition Spectrometer Channel Wavelength range nm clicking the Open button in this dialog Mater o Te aoo Nrefpenetodsv n the function output is displayed against time Both x and y axis are set to their full scale which is determined by the minimum and maximum values in the list J Display No Graphics To Speed Up Data Processing Zoom features Save Function Output Do not save Function Output In each graph the same zoom features S i aia apply as in the main window except for scaling the y axis with the mouse wheel However zooming in over the x axis while the measurement is running a
152. ngth Range CO pw akter C Woule r C yuMol ne C poules Eom 327 37 Em CC watt r C prolis n C yuMal C phat e C ploule cnt C uMal s CO y walt sr C poule e To 11001 nm Photometric Param Peak Parameters Wavelength Range C Lux Center wavelength Center intensity C FWwHM C Lumen r e From 327 37 nm Dentroid wavelength Centroid intensity Lumen e io Mom C Candela C Peak wavelength Peak intensity eae time settin amis parameter settim C Seconds Autoscale Y axis during measurements jo Display Last 1 v Minutes C Hours Preset y axis scale to a fixed level f 00 000 Display No Graphics To Speed Up Data Processing Save Function Outpu f Do not save Function Output C Save Function Output x Cancel Save ICF Load ILF o Enable Disable function The radiometric parameters can be set after clicking the Enable button o Hardware Setup The Hardware setup setting is an important parameter because as described in section 4 4 1 Background it determines the measurable radiometric and photometric parameters as well as the way these are calculated If getSphere is used it is important to know if the light source is measured inside the sphere like often is done with LED s or outside the sphere This setting can be entered after clicking the getSphere button and then inside or outside sphere o Spectrometer Ch
153. ni NS VIET Load AIG d File Menu Load Reference cccccecececeecececeeeeeeeeseeseeeeeseeeeeeeeseseeeeeeseseeeeenseneneeees File Menus Load Expertielil ies cascacesitel scien wencaneesvousbunenssicedialesiauversdwelelncabnasennidevaays File Menu Save DarK sssicrsatciniansnvatinsduadehoats iahonnestaadinaeuinintdwadaitads Va dug E Ra Ri adiu diu dd ud File Menu Save BelereliGGisveseemessexpe puse eSEE ENEN cV Pr ra eT NEEE FR VENE QN BUY UU File Menu Save Expelelilasssunwceiand i cba d xi metd zat M data cad vi GN Gf vag ecd qa Gad E data sation maa cis C File Menu Black and White Printer cccccccseeeeeeeeeeeeeeeeeeeeeeeeeseeeeeeeeeeeeeeeeeeenaes File Menu Display Saved Graph sss renis File Menu Convert Graph to ASCIL cccccccceceeeceeeeeeeeeceeeeeeeeeeesesaeseseeseegeaeseeaenass File Menu Convert Graph to ASCII Equi distance eeseseeeeeeeereeee File Menu Convert Graph to JCAMP sssssssssssessesenn mmn mener nnns mia Mq E eyes wife Is NR RT m TT mS Wavelength Calibration Coefficients sese Smoothing and SPINE scies seus tabc bs rr EAR E EENET Rt Lauf Eas Correct for Dynamic Dark getSpec 2048 3648 only Subiracioaved DarK asser zs ooe oie uei dede sie esae EE SOG EO RR OT 1 kHz Enable US
154. ntains the line of comments which may have been added to this graph The name of this second file is except for the extension the same as the name of the first file experiment name and sequence number The extension of this second file also depends on the measuring mode as shown below Extension Mode RCM scope Mode ACM Absorbance TCM Transmittance Reflectance ICM Irradiance Example suppose the name of our experiment is gts Then saving one spectrum in scope mode one in absorbance mode and two in transmittance mode results in the following files gts0001 roh spectrum data in scope mode gts0001 rcm comments for the spectrum saved in gts0001 roh gts0001 abs spectrum data in absorbance mode gts0001 acm comments for the spectrum saved in gts0001 abs gts0001 trm spectrum transmittance mode gts0001 tcm comments for the spectrum saved in gts0001 trm gts0002 trm spectrum data in transmittance mode gts0002 tcm comments for the spectrum saved in gts0002 trm info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 21 more than spectroscopy Gel spec com After leaving the application and opening getSoft the next time saving graphics in scope absorbance and transmittance mode will then result in respectively the data files gts0002 roh gts0002 ab
155. nts will not appear and with different integration times the detector is not linear 3 2 2 Setup Menu Wavelength Calibration Coefficients After clicking this option a dialog is shown in which the wavelength calibration coefficients can be changed manually Background The wavelength that corresponds to a pixel number pixnr in the detector in the spectrometer can be calculated by the following equation Intercept X pixnr X pixnr X 2pixnr X pixnr in which Intercept and X to X4 correspond E wavelength Calibration E E el xj to Intercept and the first to fourth coefficient MURS For example if we want to calculate the E actrice wavelength at pixel number 1000 using the numbers in the figure at the right the Fest Cosic 0 136492 OO wavelength becomes Gacond Costicient 5 125 06 EA 384 054 0 136492 1000 Cie KERN mf m 6 71259E 6 1E6 voccs I F ida 5 66234E 10 1E9 beret paves 013 267 nm X Cancel Restore Factor Setting info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 28 Imore than spectroscopy get Spec com The Restore Factory Settings button restores for all spectrometer channels the original wavelength calibration coefficients that were saved to the EEPROM during
156. oothing Spline OFF Saturated info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 33 more than spectroscopy Gel spec com This is useful for measurements in transmittance absorbance and irradiance mode because in these modes saturation can not be observed by looking at the number of counts like in scope mode But even in scope mode a spectrum can contain saturated pixels also when this is not directly obvious from the graph Examples are o Smoothing The maximum pixel value of a peak can be saturated but is averaged with neighbor pixels which may not be saturated o The correct for dynamic dark algorithm subtracts the dark values that are measured at the optical black pixels from the spectral data Therefore the saturation level of 16383 counts will never be reached with correct for dynamic dark ON The saturation detection in getSoft is done before the data is corrected for dynamic dark so it will also detect saturation with dynamic dark ON o Monitor resolution The CCD contains 2048 3648 pixels which is a lot more than the monitor pixels in the graph Since not each CCD pixels can be drawn at the monitor a sharp peak at one CCD pixel can be saturated although this is not visible at the monitor Use the zoom function if you want to verify if this
157. ore irradiance measurements can be done an intensity calibration is required The intensity calibration contains the data transfer function for each pixel The data transfer function is used to convert the scope data A D Counts into irradiance data in uWatt cm To be able to calculate the transfer function a calibrated light source with known output in uWatt cm nm needs to be available When saving the A D Counts with the reference light source on and off we know the relation between the A D Counts and uWatt cm 2 Caldata 2 refcal darkcal 9 Caldata Intensity of the calibrated light source at pixel n in uWatt cm from lampfile refcaln A D Counts at pixel n that were saved with the reference light source on darkcaln A D Counts at pixel n that were saved with the reference light source off ji le dark 1 Caldata sp nelle dd Baci refcal darkcal 9 When measuring the A D counts received from a light source different from the calibrated light source but of course with the same fiber optic cable and diffuser this relation can be used to measure the intensity at every pixel n in uWatt cm If sample is the measured A D counts at pixel n when looking at the sample light source and dark is the measured A D counts with the sample light source off then the equation for intensity in uWatt cm becomes If during the intensity calibration a different integration time was used e g 100 ms from the inte
158. ory Channel function 4 6 2 Calibration Settings The dialog at the right is shown after selecting Application gt Chemometry gt Settings for the first time An example calibration file exampleO cal has been created in the folder chem which holds the data that is pees in the dialog Note that the xem is only illustrative You need to measure a number of samples with known concentration to create your own calibration file s as described in section 4 6 2 1 After a calibration has been saved it will be loaded automatically the next time the application is started Once a calibration has been saved it can be loaded later on and modified if needed Section 4 6 2 2 describes how to modify a previous saved calibration file 4 6 2 1 Start New Calibration ioi xj Select Variables Actual Points Channel Waste Actual Line m New Points Peak at 505 0 nm New Line Width 10 0 nm Conc Unit mmol A S 0 1 0 2 0 3 0 4 0 5 0 6 Start New Calibration Clear Data Ries EE e S Le Calibration Points y Calibration Line Coefficients First Order Linear Default C CEN onc onc Int t roenEOU C Second Order Quadratic jans 1 0605E 001 SU 0 000 0 1 06 Coef 1 4 9923E 002 Actual Calibration Filename Master 0 2001 100 000 100 451 Coef 2 0 0000E 000 example0 cal Load 0 398 200 000 198 800 StDev rom 0 602 300 000 300 643 Read Absorbance for point
159. ough outliers Actual Points Actual Line m Nev Points New Line 0 02 0 4 06 08 Absorbance Adding deleting calibration points Adding and deleting calibration points from to the actual calibration line is very simple and very well visualized in the graphical display To add new calibration points to a calibration just load the calibration file move the cursor to the next row in the table row 5 in our example fill in the sample concentration in the center column and read the absorbance into the first column by clicking the Read Absorbance for point 5 button A new red point will mark the calibration point in the graph New points can be added up to 30 standard samples The Calculate New Calibration Line will display a new red line next to the actual blue calibration line before raising the dialog with the question if the new calibration data should be saved or not This may be helpful to see if the new points are correctly measured or that one or more new points induce a strong increase of the standard deviation For example in the figure at the right the error was caused by typing in the value of 60 in the center column instead of the correct value of 40 To delete one or more calibration points from the actual calibration just delete the value for that point in the left or second column info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0
160. p 1 2 3 10 11 12 Tel Fax otart the getSoft software and click the Start button in the main window Connect a fiber to the spectrometer input port otart the absolute irradiance application software by clicking the menu option Application gt Absolute Irradiance Measurement Click the Perform Intensity Calibration button select the spectrometer channel that will be calibrated the calibration lamp file and enter the diameter of the fiber cosine corrector or integrating sphere sample port that is used as described in section 4 4 4 Application Absolute Irradiance Turn on the reference light source e g getLight HAL CAL or getLight HAL CAL ISP If a cosine corrector is used at the end of the fiber mount it directly on the reference light source If an integrating sphere is used at the end of the fiber put the integrating sphere sample port over the light output port Verify that the calibration lamp is ON for at least 15 minutes and click the Start Intensity Calibration button Try to adjust the integration time while looking at the reference light such that the maximum count over the wavelength range is around 14000 counts It is also possible to let getSoft search for an optimal integration time by clicking the A0 button Adjust the smoothing parameter to optimize smoothing for the fiber slit diameter that is used If a good reference signal is displayed click the white Sav
161. p gt Options gt Auto configure Integration time If measurements are done in a mode in which reference and dark data are required all modes except scope mode then a new reference and dark spectra have to be saved after the integration time has been changed Average With this option the number of scans to average can be set A spectrum will be displayed after every scans This spectrum is the average of the scans Wavelength nm The wavelength shows the position of the cursor which becomes visible if the cursor button is down The amplitude of the signal which is given in the status bar at the bottom of the main window is the amplitude at the wavelength shown in this field Change IntTime and or Averages Sin Ea Multi channel USB2 onl PE IntTine and or cc olx ulti channel US id to All Spectrometer Initime ms Nrot Averages For multi channel USB2 Tothiba UA 20000 j1 spectrometers the integration time and averaging can Sony LI coo A be set for all channels at the same value by clicking to X Conca info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 15 more than spectroscopy Gel spec CO m AI If the dashed box is clicked different integration time and averaging can be set pe
162. pectrum as Reference if TTL signal is high pin 8 For absorbance or reflectance transmittance measurements it is recommended to update the reference data regularly to exclude effects like drift in the light source or e g temperature effects of the detector or in the optical bench In most cases reference is saved manually by clicking the white Reference button This reference data will be saved automatically if this external trigger option is selected and the status of pin 8 is HIGH 5V at the moment that a new spectrum is available For the required TTL signal duration and timing see the above description for saving spectra at high TTL signal External Hardware Trigger perform a single scan at rising edge of TTL pulse pin 4 By selecting the external hardware trigger option the data acquisition mode of the spectrometer is changed There will be no data acquisition until a TTL pulse is received at pin 4 of the DB15 connector The delay between the rising edge of the TTL pulse and the start of the integration time cycle depends on the spectrometer type as shown in the table below Spectrometer Type Minimum Delay s Maximum Delay s getSpec 1 02 1000 1500 getSpec 2048 S N 040505481 2000 2500 For example the integration time as set in getSoft starts for a standard getSpec 102 after 1 0 to 1 5 ms after receiving the TTL pulse at pin 4 of the DB15 connector After the integration time expires the data is processed by
163. pin cable IC DB15 2 and for the USB2 platform a 26 pin to 15 pin cable IC DB26 2 The TTL switch at the light source needs to be in TTL position In getSoft the menu option Save automatic dark by TTL Shutter needs to be enabled in the menu Setup gt Options If this option is enabled the TTL will close the shutter of the light source at the moment the dark data is saved After the dark has been saved the shutter will be opened automatically 3 2 9 7 Setup Menu Options External Trigger Setting USB1 platform The DB15 connector on the getSpec 2048 spectrometer uses two pins for digital IN communication pin 4 and 8 Pin 4 is used for the Hardware Trigger mode and pin 8 is a regular digital i in TUITION status can be polled In getSoft two pamm E E x functions have been defined for using the digital in at pin 8 A lot more customer specific features can be easily implemented For generating a 5V signal at pin 8 manually the Automatically save on Hardware Trigger handheld pushbutton cable IC Extrig 2 may be ordered or if the Automatic Save Dark by TTL shutter option needs to be supported as well an IC DB15 Extrig 2 Y cable can be ordered The different options which are shown in the dialog above are described below Perform scans continuously save first available spectrum if TTL signal is high pin 8 Perform scans continuously save first available spectrum as Reference if TTL
164. ple triple view mode will automatically have been increased to 0005 0006 and 0007 for resp Master Slave1 and Slavea 3 1 8 File Menu Print After selecting the print menu option the at ren eam background colors in the graphical region Ars EATI ces eec cor will become white If the menu option Black and White Printer see next section has been marked the line style for the spectra will also change from colored to black A dialog will be shown in which the title for the printout can be entered In the next window the printer settings can be changed e g portrait or landscape printing printing quality After clicking OK in the printer settings dialog the graph will be printed and the original graph colors will be restored on the monitor I e mi Lime xx mam me PU info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 22 Imore than spectroscopy Gel Spec com 3 1 9 File Menu Black and White Printer The default setting in getSoft is to print the spectra in the same color as they appear on the monitor If a color printer is not available the menu option Black and White Printer can be enabled If this option is enabled different line styles will be printed if more than one spectrum is displayed e g dash das
165. py Gel Spec com ia 0 2 Launching the software getSoft can be started from Windows start menu Under Start gt Programs the group getSpec Software was added This group contains two icons With the getSpec chameleon icon getSoft is started The getSoft Help icon can be used to activate the getSoft help files these help files can also be activated from the help menu after starting getSoft R Adobe LS Microsoft office 4 Cm Desumenkte k WinRAR F Bees 29 zubeh r F 5 Eirstellungen i CS cpr spec3z E S Suchen e getspecRAYv v4 1 getSpec COLOR Vi a i Hite Gerspec Software I pa EAT LE HTML Help workshop f USB1 platform After starting getSoft the dialog at the right a Spectrometer detected on USB Number of Pixels 2048 will be shown to indicate that the USB A connection has been detected a similar dialog will be shown if the serial RS 232 interface is used Information E x If more than one getSpec 2048 spectrometer is connected to the PC the dialog at the right will be shown which allows to select the spectrometer serial number for which you want to use getSoft With USB1 platform spectrometers you can run multiple spectrometers simultaneously just by restarting getSoft multiple times After clicking the OK button the main window is displayed O x C 031101451 C o20s00641 x Cancel USB2 platform l After starting the
166. r or getSphere 6000 10000 or 15000 micron for respectively getSphere 30 getSphere 50 and getSphere 80 needs to be entered in micron It is important that the hardware setup that is used during the calibration is NL Logo E TN Smoothing NN the same as the hardware setup in the ir radiance measurements 4 4 4 1 Start Intensity Calibration Verify that the calibration is ON for at least 15 minutes and that the 35 10 000 hardware has been setup correctly Then click the button Start Calibration As a result the Scope data A D Counts for the selected spectrometer channel will be displayed graphically A D Counts M Set the smoothing parameter to optimize smoothing for the fiber slit 400 500 600 700 800 300 1000 1100 Wavelength nm info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 88 more than spectroscopy Gel Spec com diameter used see also section 3 2 3 Setup Menu Smoothing and Spline Set the integration time such that a good reference signal maximum around 14000 15000 counts is measured It is also possible to let getSoft search for an optimal integration time by clicking the Auto Configure Integration Time ACY button Set the number of scans to average to a high number to reduce the
167. r channel 2 4 Graphical region The graphical region displays the data in a xy diagram with the wavelength in nanometers at the x axis and at the y axis the detector counts After loading or saving a reference and dark spectrum other units can be selected at the y axis Absorbance Units Percentage Transmittance or Relative Irradiance HEU ME HE SR UC EDO Line Properties Edit Comment Display saved Graph and Line style editor Tr By clicking on the legend with the right mouse button 17 Miabi earlier saved multiple spectra can be displayed BONES SUN New in specSoft 7 is that displayed graphs can be Color width 4 deleted or properties of the displayed graphs such as line style or color or comments can be changed Round 7 Cancel This is done by clicking with the right mouse button on the line in the graphical display A small line edit box will occur Style Solid v Now the line can be deactivated or the line properties can be changed as depicted in the border editor or the comments can be edited Zoom features Zoom in Select a region to be expanded to the full graph To select this region click the left mouse button in the white graphical region and drag it downwards and to the right After releasing the left mouse button within the graphic display both the x and y axis will be rescaled to the new values of the selected region info getSpec com Tel 49 0 351 871 8423 T l
168. ral calculations are enabled after they have been defined in the dialog that is shown at the right This dialog is displayed after activating the menu option Setup gt Options gt Integrals In the example below two integrals have been defined the first 2 are marked by the Enable checkbox in the first column In the second column a spectrum can be chosen out of all spectra that are at that moment displayed in the main window in getSoft info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 36 more than spectroscopy A full list of the spectra to choose from this includes earlier saved spectra like integral 2 in the example is displayed after clicking on the Each Mueckum Wavelength Range Multiply with x i integral Rampe i alg x Enter mar elengih rares and cpeciom bor miegal cak ulahn arrow button at the right side of the Le Mam gt From fi Tm fC second column In the third and 2 i hrm Fahin afea m un fourth column the wavelength range x Oo neue Foafoa tafo mm fioo can be entered over which the r Maste Fom or in 11160 nm um integral should be calculated Finally S pow Ef faaji defen nm uon a multiplication factor can be entered DLE s coo RES im ULL ue T Mase Foha to Thm fion for scalin
169. ransmittance mode the Reflectance name will shown at the y axis o The caption at the Reflectance Transmittance button will change from T to R and the corresponding menu option under View will change from Transmittance mode to Heflectance mode info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 49 more than spectroscopy Gel Spec com 3 3 View Menu E GetSofto QFvll 2005 GetSpec File Setup EVS Annlcation Help Sto scope Mode A Stop Absorbance Made T I Integration ll Transmittance Mode D dessin SS Grace Reset Autoscale Y axis Change Graph Scale Goto Preset Scale 14 w Gro Enable i 3 9 1 View Menu Scope Mode The display is set to Scope Mode showing a real time raw data signal with on the y axes the read out of the AD converter and on the x axes the calculated wavelength 3 3 2 View Menu Absorbance Mode In Absorbance Mode the absorbance at pixel n is calculated using the current sample reference and dark data sets in the following equation 2 2 A 2 jog ol a dark 9 ref dark 9 3 3 3 View Menu Transmittance Reflectance Mode In Transmittance Mode the transmittance at pixel n is calculated using the current sample reference and dark data sets in the following equation r 100 53
170. rk spectrum black button after changing the integration time 4 46 Time Series Measurement After an intensity calibration has been performed or loaded the Irradiance Time Series TAB becomes available in the Irradiance Settings dialog By clicking the TAB the Irradiance Time series settings are displayed With time series irradiance measurements up to 8 radiometric parameters can be followed simultaneously in a graph against time The functions F1 to F8 can be selected by clicking the corresponding TAB sheet at the top of the Time Series settings dialog Furthermore a number of general function independent parameters can be entered below of the function TAB sheets The following features can be set separately for all functions F1 to F8 info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 96 more than spectroscopy ET trradiance Settings Calibration Irradiance Chart Irradiance Time Series Fi F2 r3 r es re F r F Channe Colorimetric Parameters CIE Standard Observe f Enable v dw Cu Dominant Wav 2 degrees l Master Cy Cv Comp Dom Way C Disable E Color Temp Purity 10 degrees Hardware setup Detector type f COUYAVIS ar Fiber C AvaSphere Radiometric Parameters Wavele
171. robe to the light source and to the spectrometer input port s and set up the experiment for taking a reference spectrum Adjust the smoothing parameters in the setup menu section 3 2 3 to optimize smoothing for the fiber slit diameter that is used for USB2 platform getSpec s optimal smoothing is preset and stored on board in the EEPROM Now turn on the light source Usually some sort of spectrum may be seen on the screen but it is possible that too much or too little light reaches the spectrometer at the present data collection settings Too much light means that over a certain wavelength range the signal is saturated shown as a straight line at the maximum counts and the appearance of the label Saturated in the status bar of the spectrometer channel This can usually be solved by a shorter integration time The integration time can be changed in the main window in the white box below the Start Stop button If getSoft is collecting data the Start Stop button shows a red Stop and the integration time box is gray indicating that it cannot be changed USB1 After clicking the Stop button the data acquisition stops and the integration time can be changed The result of the changed integration time can be viewed after clicking the green Start button Try to adjust the integration time such that the maximum count over the wavelength range is around 14500 counts When at minimum integration the signal is still too high an att
172. rt Measuring menu option 4 1 2 History Application Start Measuring This option displays the output against time for the history functions that have been defined in the History Channel Function Entry dialog box If the option Display no graphics to speed up data processing has been marked in the function entry dialog the function output will be shown by numbers only which are updated each time a new scan is saved to the output file There are six buttons at the top of this window an Exit button a Pause Start button a Save Reference button a Save Dark button an Info button and a Print button If the red Exit button is clicked the time measurements are ended and the main window and menu is activated again The yellow Pause button can be used to stop the time measurements temporarily After clicking the Pause button data acquisition stops and the text on the button changes to a green Start If the Start button is clicked data acquisition is activated again and the text changes back to the yellow Pause again info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 61 more than spectroscopy The Save err Reference and A Save Dark buttons E f MIO have the same Toa cg Ma n a M e A APA PLU gp
173. rtmund Sofia Seite 10 more than spectroscopy shows the corresponding values of wavelength and amplitude in the main screen By clicking the red Stop button the data acquisition is stopped and the last acquired spectrum is shown in static mode The data acquisition can be started again by clicking the same button which now shows a green Start To save the spectrum in the mode chosen before choose File gt Save gt Experiment from the menu or click the Save Experiment button from the button bar 9 To improve the signal noise ratio a number of spectra can be averaged To do this the value in the white average box in the main window next to integration time can be increased The value can be changed only in static mode When getSoft is acquiring data the average box becomes gray info getSpec com T l Fax Tel Fax 449 0 351 871 8423 449 0 351 871 8465 433 0 4 72 80 82 02 433 0 4 72 80 82 43 Tel Fax 449 0 231 974 2285 449 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 11 more than spectroscopy gel Spec om 2 Main Window 2 1 Menu bar File Setup View Application Help The menus and submenus are described in section 3 2 2 Button bar For the USB1 platform Sa Bil 2 a3 SATI Bets IE B I n XY d o z For the USB2 platform soo MSS ATI OSS foe isl Start Start Stop button The Start Stop button c
174. s experiment has been ended as described in section 4 1 3 History Application Display Saved History Graph Function Independent Parameters Save Function Output The results of a time series experiment will be saved to an ASCII file if the radio button Save Function Output has been selected A number of seconds between saving can be entered for data reduction in case measurements are carried out over long periods Entering a value of zero results in saving every scan The name of the file to which the data will be saved can be changed after clicking the Change Output File button Last option in the Save Function Output box is to enable or disable the possibility to create a backup file during the measurements If this option is enabled getSoft will create a backup file with the same info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 60 more than spectroscopy Gell Spec com filename but with the extension bak also in ASCII This backup file is updated every scan and can be used in case the filename that has been selected has failed to save the data for instance because of a power failure during the measuremenis Save HCF Load HCF buttons getSoft automatically saves all parameters function definition save options to the file h
175. s and gts0003 trm as well as the comment files gts0002 rcm gts0002 acm and gts0003 tcm Before saving the name of the graphic file is displayed in the status bar at the bottom of the screen After saving the sequence number is automatically incremented by one Saving graphic files if multiple spectrometer channels are enabled If graphic files are saved while more than one channel is viewed at the same time see option View Menu Channel only for USB1 platform then the graphic filename for each channel that is displayed gets a different sequence number For example the result of saving one experiment in triple view mode is three graphic data files and three comment files for instance gts0002 roh spectrum data in scope mode e g spectrometer 1 or Master Channel gts0003 roh spectrum data in scope mode e g spectrometer 2 or Slave1 Channel gts0004 roh spectrum data in scope mode e g spectrometer 3 or Slave2 Channel gts0002 rcm comments for the spectrum saved in gts0002 roh gts0003 rcm comments for the spectrum saved in gts0003 roh gts0004 rcm comments for the spectrum saved in gts0004 roh For each channel a different comment line can be entered It is easier to select the graphic files later with the option File gt Display Saved Graph all comment lines start with a short name for the channel at which the graph has been saved M for Master S1 for Slave1 and so on After saving the sequence numbers in this exam
176. s shown in the dialog at the right Pair Device F E T eM r 000 Bb Yan Pebketeoih Py Gerace Took Hap The getSpec will be displayed by an duse a N icon in the ring with an address and a pe we Dea Y 2 Nude question mark Right clicking this icon shows the menu displayed in the figure at the right Select the Pair Device menu option Enter 0 in the Bluetooth passkey dialog and click OK Establish data communication Select the Refresh Services menu option after right clicking the device Meses Cr 12 F3 009 HDD Das ka name search finished info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 7 m ore than spectroscopy gelsoeccom icon As a result the Bluetooth Serial Port Service icon will be marked Right clicking the Bluetooth device icon again now shows a new menu option Connect Quick Connect a i i X Automatic connection established Do you want to 4 T3 After clicking Connect Bluetooth serial P connect to thia device automatically when Windows port device a virtual serial port number applications open the serial port T is assigned for this connection Click Serial Part COMB Yes in the dialog at the right to Remote Device Bluetooth Device connect automatically if Windows v
177. scopy Gell spec com exist It will be created In the next dialog the name for the program manager group can be changed The default name for this is getSpec Software After this the Start Installation dialog is shown After clicking the Next button the installation program starts installing files After all files have been installed the Installation Complete dialog shows up Restart the computer before starting getSoft Connecting the hardware Connect the USB connector to an USB port on your computer with the supplied USB cable If the spectrometer type is a getSpec SPU put the switch into SPU position If the getSpec 2048 spectrometer requires a 12 V power supply connect the getSpec 2048 to the 12 V power supply Windows will display the Found New Hardware USB device dialog and starts searching for the driver This can take a few minutes Under Windows XP a window will be displayed that the getspec Found Mew Hardware Wizard opectrometer board has not passed Fleece wa miale the wizard installs the software Windows Logo testing to verify ME Mrd compatibility with Windows XP which indicates that the manufacturer of the USB driver did Thee scd ve pu sre raid Pai his Perdre dwane 5 pecticmsier boad not send it to Microsoft and also paid E AR a aE assi E ied Eas Microsoft for testing of this driver as r Lontinuing sour installation of this eolivware mars mpar i j w destahik
178. seful for applications in which the output of line sources like laser diodes is displayed or for other applications which require a high resolution Note that for the getSpec 2048 with 2048 pixels the effect of spline interpolation is not visible if the data is shown at full scale The monitor resolution is much less than 2048 pixels The effect of spline interpolation can only be visualized if the number of detector pixels that are displayed is smaller than the number of monitor pixels S at the x axis Smoothing To get a smoother spectrum without losing information it is important to set in the software the right smoothing parameter The optimal smoothing parameter depends on the distance between the pixels at the detector array and the light beam that enters the spectrometer For the getSpec 2048 the distance between the pixels on the CCD array is 14 micron info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 29 more than spectroscopy get Spec com With a 200 micron fiber no slit installed connected the optical pixel resolution is about 14 3 CCD pixels With a smoothing parameter set to 7 each pixel will be averaged with 7 left and 7 right neighbor pixels Averaging over 15 pixels with a pitch distance between the CCD pixels of 1
179. shown for the reference color If time measurement data have been saved the resulting ASCII file can be displayed graphically by selecting this file in the dialog that is shown after clicking the menu option Application gt History gt Display Saved History Graph After clicking the Open button in this dialog the function output is displayed against time Both x and y axis are set to their full scale which is determined by the minimum and maximum values in the list Zoom features In each graph the same zoom features apply as in the main window except for scaling the y axis with the mouse wheel However zooming in over the x axis while the measurement is running and the x axis is already scrolling will not be possible because in that case the x axis is updated with each new scan Clicking the Pause button to take a snapshot will solve this problem Zoom in Select a region to be expanded to the full graph To select this region click the left mouse button in the white graphics region and drag it downwards and to the right After releasing the left mouse button both the x and y axis will be rescaled to the new values of the selected region Zoom out Drag with the left mouse button within the white graphics region but in stead of dragging the mouse downwards and to the right drag it into another direction After releasing the mouse button both the x and y axis will be reset to their default values Move X Y Dragging
180. sity 2 2651 Peak Wavelength unit nWatt Wavelength at the maximum spectral power Centroid Wavelength The total spectral power left and right from the centroid wavelength integral is the same info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 80 more than spectroscopy Gel spec com Radiometry The radiometric parameters can be grouped into three categories o Radiant Flux nWatt The radiant flux is the total optical power emitted from a source in all directions The best way to measure the power emitted by a source is to measure the source inside an integrating sphere This is often done when measuring LED s It is also possible to calculate the flux of a source by measuring the irradiance at the surface of the diffuser cosine corrector or integrating sphere sample port at a certain distance from a light source An important assumption in this calculation is that the source should be isotropic and the distance between diffuser and source should be greater than five times the largest dimension of the source approximation of point source o Radiant Intensity uWatt sr The radiant intensity is the optical power per unit solid angle It is used to quantify the optical power that is emitted by a source into a certain direction Radiant int
181. sssseseseeen nnnm mener nen neris 86 4 4 4 miziaens a iVAe ee ETT T C ET TT 87 4 4 5 AGI AICS WANE PEE 90 4 4 6 THOME Series MEASULCH IONE scsauatessexedqeizit itus sm MES Dri pde ad taxes rU BUE rita iaa ant 96 4 5 Yee eculequimi BOUM 100 4 5 1 CLEC SOUR DO D didis invo pRO IE RERUM E IE Gul d I RN ENEUEE RUE DKDMDN NES Rr ORTU DENM 100 4 5 2 zal CS OO MN TETTTE UT 101 4 5 3 OE IUTIO a ee ee LI produce tomm p M ee eee eee ToU re er dE 101 4 5 4 ti OU Gahan t i A9 105 4 5 5 OP OU TOE M te 105 4 5 6 Limitations and Optimization Notes cccccecececeeeeeeeeeceeeeeeeeeeeeeeeeeeeeeeaeeeeeneeeees 106 4 6 CRONO I T T E U c UU m aa sentinan E dene Mentualweniseausuaan 107 4 6 1 Quick Start How to make concentration measurements with getSoft Chem 107 4 6 2 ONION ANON SENGS E T E I TT 108 4 0 2 1 Start New GallDEaI OTI seesotuatcba nessen adorare ore deg vba Spit ad Iun Evae na 108 40 2 2 MOodID IDO a CANDAN usce ptus a EUici assis aae E Pair Goa i urba a 110 4 6 3 Enabling the applIGA HOT s ussuxacnei n barack Reo ux ioa Ex xa art tentis Bop mcr OR ao cuarta ecu 111 5 HELP 112 info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 3
182. t with the extension sat For example if a graph in absorbance mode is saved to test0001 abs and if this spectrum contains saturated data then additional lines will be written to the textfile test sat If time series measurements color measurements irradiance measurements are saved to file the data will also be checked on saturation and if saturation occurs additional lines will be written to a logfile with the same filename as the measurement results but with the extension sat e g History sat The files with extension sat can be openend with any textfile editor for example Notepad 3 2 9 2 Setup Menu Options Full Width Half Max The Full Width Half Maximum of a peak is the bandwidth in nanometers for which the intensity i i i i f i FWHM enabled Please make sure that the menu option IS higher than half of the maximum Intensity of that i Setup Substract Saved Dark has been enabled Use the peak The FWHM can be calculated in Scope or II Irradiance Mode This utility is used mostly measuring the output of laser diodes During FWHM calculations the intensity needs to be corrected for the dark data Therefore it is recommended to enable the option Subtract info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 35 more than spectroscopy
183. ted by clicking the OK button The irradiance chart and time series settings will be described in sections 4 4 5 and 4 4 6 4 4 5 irradiance Chart 400 500 600 700 300 900 4 000 1 100 Wavelength nm 4 4 5 1 Irradiance Chart Settings After an intensity calibration has been performed or loaded the Irradiance Chart TAB becomes available in the Irradiance Settings dialog By clicking the TAB the Irradiance Chart settings are displayed The figure at the right shows the default settings for Spectrometer Channel Hardware Setup Parameter ES radiance Settings Calkeahon mhinice Cha Inadiance Tie group Graphics and Save Settings Spechromeber Channel The Spectrometer Channel setting can be changed if for a multi Menta z channel spectrometer system calibrations have been performed or Hardwere setup loaded for more than one channel E E E GRIS phes The Hardware setup setting is an important parameter because as described in section 4 4 1 Background it determines the radiometric and photometric parameters that can be measured as EL well as the way these are calculated If getSphere is used it is fcx important to know if the light source is measured inside the sphere cs Boe like often is done with LED s or outside the sphere This setting Pesk Measurement can be entered after clicking the getSphere button and then inside Graphics or outside sphere e papane dean The Parameter group setting determines
184. ted noon sunlight and Simulated overcast sky daylight Note that there is no relation between the illuminant chosen here and the light source that is used for measuring the color of an object The selected illuminant is used in calculation of the color parameters These color parameters will be different if for info getSpec com 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 72 more than spectroscopy Gel spec com example A will be selected instead of D65 just as the color of an object will look different in average daylight or in incandescent light 2854 K blackbody o CIE Standard Observer The original CIE standard observer functions x y and z were defined in 1931 and are known as the 2 degrees standard observer values The 2 degrees corresponds with the angle of vision that was used in the experiments to determine these standard observer values In 1964 the CIE recommended the use of different observer values for a higher correlation with visual perception for large samples These are known as the 1964 supplementary standard observer or 10 degrees observer values Both standards are still in use and in getSoft the CIE standard observer can be selected by clicking one of the radio buttons The default is 2 degrees o Function display settings For each function one of th
185. tegration time in getSoft To increase light intensity the number of pulses per integration interval should be increased The maximum frequency at which the getLight XE operates is 100 Hz This means that the minimum integration time for 1 pulse per scan is 10 ms When setting the number of pulses e g to 3 the minimum integration time becomes 30 ms It is recommended to keep the integration time as low as possible to avoid unnecessary increase of noise USB1 platform The getLight 2000 needs to be attached to the getSpec by connecting an IC DB15 2 interface cable to the high density 15 pole Sub D connectors at the getSpec and getLight XE If used with an old XE 2000 the XE 2000 has the possibility to switch between Single Flash and Multi Flash in that case there will be a switch at the back side of the XE 2000 make sure that the switch is always in the Single Flash position when using the XE 2000 with an getSpec spectrometer in Single Flash mode the XE 2000 is triggered at pin 1 in Multi Flash mode at pin 2 WA x When clicking the Strobe Enable menu option Enter the number of pulses a dialog is shown in which this number of pulses Per integration interval can be set If this menu option is preceded by a checkmark the strobe control function has been enabled To disable the strobe simply click the menu option when preceded by a checkmark Strobe getLight XE Control USB2 platform The getLight XE needs to be atta
186. ternative the incoming light can be attenuated e g by using an attenuator FOA Inline or neutral density filter 4 2 1 Wavelength Calibration Perform New Calibration The procedure to perform an auto calibration is as follows Tel Fax Connect the fiber to the getLight CAL light source and to the spectrometer channel to be calibrated Choose a suitable integration time so as not to saturate the detector The peaks can be seen in scope mode Select the menu option Application gt Wavelength Calibration gt Perform New Calibration The auto calibration routine will now search for a number of peaks initially it will look for 5 peaks in the data from the master channel The spectrometer channel and the number of peaks to look for can be altered A new search can be Calibration Select Channel Master Peaks ir Max Fit Order 2 t 3 Iv include 2nd order peaks Find Peaks Calculate Close Present 365 06 404 60 407 71 435 87 546 09 577 10 578 98 595 46 750 44 763 49 772 41 Standard 365 01 404 66 407 78 435 84 546 08 579 07 686 54 750 38 763 51 772 40 loj xl A performed by pressing the Find Peaks button The peaks are shown in 3 columns The first column shows the position of the peaks found The second column shows the position of a suggested standard peak if available The last column lists the
187. the extension irr The irradiance spectrum files can be saved and loaded from the irradiance chart dialog If all settings have been entered the measurements can be started by clicking the OK button To be able to measure the right A D counts corrected for the dark spectrum a warning will be displayed if a dark spectrum has not been saved Information Bp x d Dark data has not been saved For Master Block the light input then Save Dark by clicking the black button 4 4 5 2 Irradiance Chart Display The irradiance chart displays the graphics and output parameters that were selected in the settings dialog Moreover up to ten radiometric parameters can be selected each with a user defined wavelength range The measurement results can be saved and earlier saved spectra can be graphically displayed and compared to the measured spectrum The integration time and number of scans to average can be changed to optimize the AD Counts for each sample Fra HL e tol Hoc ak um nm me r iim The figure above shows a result for a typical LED measurement inside getSphere If the light source is measured outside the sphere or with a cosine corrector and the radiant flux uWatt radiant intensity uWatt sr luminous flux Lumens or luminous intensity info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 4
188. the integration time outside sphere or uWatt cm Power received per square centimeter CC outside sphere or CC Energy cm uJoule cm Energy received per square centimeter calculated by multiplication of irradiance with the integration time outside sphere or Photon Flux m umol s m Photons received per second and per square CC meter see for calculation previous page outside sphere or Photon Flux umol s Photons received per second at diffuser CC surface outside sphere or Photons m umol m Photons received per square meter during CC one integration time cycle outside sphere or Photons umol Photons received at diffuser surface during CC one integration time cycle Tel Fax 449 0 351 871 8423 449 0 351 871 8465 info getSpec com T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 83 more than spectroscopy Gel Spec com Photometry Photometry is the measurement of visible light Unlike radiometry it is not a purely physical measurement and is calculated considering a standard human visual perception This is attained by multiplying the radiometric data by the luminous efficacy curve see figure below and integrating the product over the visible range 380 780 nm Luminous efficacy curve 700 600 500 400 300 Luminous efficacy lumens watt 200 10
189. the line number A dialog will be shown in which the radiometric parameter Su Enc Eje e he eer latch p and wavelength range can be specified The wavelength range is the spectral range ea LS neem over which the radiometric output will be eM integrated For example by specifying the None UV C UV B and UV A wavelength ranges at Irradiance n Watt cm three different lines the irradiance for these Power received n Watt spectral ranges can me measured Energy cm HJoule cm simultaneously assuming the spectrometer Energy received pdJoule wavelength range includes the specified Photon Flux m uHo1 8 m ranges and has also been calibrated for USO ola these ranges Note that the available parameters depend on the settings in the irradiance chart settings dialog For example to be able to measure the radiant intensity the Calculate Power and or Energy emitted by a source option should be enabled info getSpec com T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 93 more than spectroscopy Gel Spec com Chromaticity Diagram 16 000 4 Treadiance Chart Measurement Eafe Bi x a 14 000 3 en ae The chromaticity a Detector ape get phere source inside spese I I 68 000 Dissis pipi gest pere LXXX ctn visualize the F 6 000 4 colorimetric else dvor pel lire Mende Intensity Calixati
190. the reference color and the actual measured L a b values 4 3 2 Color Measurement After selecting the menu option Application gt Color Measurement a dialog is displayed in which the input parameters can be set The dialog shows two tabbed pages one is called LABChart and shows the input parameters for displaying the color measurements in a color info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 67 Imore than spectroscopy gel Spec com chart the other one is called Time Series and shows all input parameters for displaying up to eight color parameters in a graph versus time The input parameters will be described in detail in sections 4 3 2 1 LABChart and 4 3 2 2 Time Series The color measurements are started by clicking the OK button getSoft returns to the main window if the Cancel button is clicked 4 3 2 1 LABChart FE Ini xl LABChart Time Series S Ch l Mllluminant CIE Standard Observer DOCOEAE UT C D55 C D75 2 degrees Master C B C D50 D65 C 10 degrees r Display Settings Display following Color Parameters v Display LABChart Iv L Iv X Iv H Jv dL M Display Reference Color Panel vV a lv Y lv C v da w Display Measured Color Panel Iv b lv z Jv dE vw db r
191. ts 8 Save the reference data This is done by File gt Save gt Reference from the menu or by clicking the Save Reference button 9 Note that the whole operation of saving a reference file does not need to be carried out each time a new experiment is started The reference data are saved in a file called ref dat and can be loaded next time by choosing the option File gt Load gt Reference After saving or loading reference and dark the irradiance mode can be chosen by clicking the I button or by the menu option View gt Irradiance Mode First a message box appears in which the value of the color temperature in Kelvin of the light source that has been used as a reference can be entered If light from the reference light source is viewed the Planck curve for the color temperature of the reference light source is displayed The maximum of the reference Planck curve has been set to 100 3 3 5 View Menu Channel only for USB1 platform After selecting this option a dialog is shown in which the channels to be displayed can be selected Depending on the number of available channels in the spectrometer system that is used up to 8 spectrometer channels can be selected If displaying graphs that were saved before File gt Display Saved Graph the active channels also remain visible to be able to measure online against a saved graph background To view only the saved graphs all active channels need to be unse
192. uct from the list will 82240 7570 72330 be ignored 71 740 26650 53440 Ln UT 50110 59160 48770 aes By clicking the OK button the current 8 838 nm 52 7i 61 310 43 720 fd reference color in the LABChart settings 10 E70 nm 33290 3 20 AN dialog is changed to the color which has T E84 nm 29380 3290 220 NM been selected To add the current reference color TT i 4 UK X Cancel specified in the LABChart settings info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 449 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 70 Imore than spectroscopy Gel Spec com ia dialog to the database file the Add button below of the select button can be clicked If the ProductlD already exists a dialog will be shown in which the choice can be made to overwrite the old color parameters with the new ones or to keep the old values for this productlD As an alternative a reference color can be added to the database during the online measurements This can be done only if in the LABChart settings dialog the option Add new reference colors to DB when measuring is enabled After all settings are filled in correctly the Exit jm M ee uj measurements can be started by clicking the OK button or using the Enter key on the keyboard A shortcut to start measuring with LABChart directl
193. uously update FOS reference measure sample on switch Update FOS reference every En seconds FOS Reference Sample Reference x Cancel 400 500 B Tog a 900 Wavelength nin If the interface cable between getSpec 2048 and FOS 2 and the Power Supply are properly connected the FOS 2 will close top sample channel and open the bottom reference channel As long as the FOS settings dialog is not closed the FOS 2 remains in this position If you want to use the second method to correct for drift by saving a new reference see previous page click the checkbox FOS Reference Sample Reference at the bottom of the dialog When using the first correction method the integration time for the FOS reference channel can be set to a different value as for the FOS sample channel Remember to save a new dark and reference for the FOS reference channel after changing the integration time Determine a good integration time and save a FOS reference by clicking the white button Then switch off the light source and save a FOS dark spectrum by clicking the black button The FOS correction mode as shown in the FOS settings dialog can be set to Software or to Auto This setting determines when getSoft should switch channels during the measurements If set to Software the user determines when to Switch channels The left dialog will be FOS 2 Ref Channel E shown after the OK button has been clicked in the FOS
194. urement The intensity calibration as performed under point 9 can be loaded in future experiments by selecting the option Load Intensity Calibration as described below under Quick Start info getSpec com 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 85 more than spectroscopy Gell Spec com 2 For the USB2 spectrometers the intensity calibration that has been stored in the EEPROM is loaded automatically during the initialization of getSoft After loading an intensity calibration a dark spectrum needs to be saved before switching to Irradiance mode Quick Start 2 Perform absolute irradiance measurements by loading an intensity calibration 1 Start the getSoft software and click the Start button in the main window 2 Connect the same fiber and diffuser or integrating sphere that was used during the intensity calibration that will be loaded to the spectrometer input port 3 Start the absolute irradiance application software by clicking the menu option Application gt Absolute Irradiance Measurement If an intensity calibration has been stored in EEPROM before USB2 platform only this will be auto loaded at getSoft program initialization Click the Load Intensity Calibration button A dialog shows up in which the intensity calibration file can be selected
195. viewed edited and or saved under a different filename by clicking the View Edit Lamp Data button 20001 20 DH ae 29 Le j j SR Gees A dialog with the intensity versus wavelength values for the selected calibration light source is shown as well as a graph in which the intensity is displayed versus wavelength info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 87 more than spectroscopy gel Spec com Ewie Edit Lamp Calibration Data I inl x a m 2 5i xu m 1201 3S ari 00 JEsi EINER d 4x in aom no D ATdbz LE 10 B5 1S 110 00 2 En im na B 0581 1 i350 noi Ta 71178 hii 00 1234572 i Ki 141038 1453 Do 15 23071 Hn 2a 300 400 50 E zn a au 100 1100 Bem tee id If needed the data can be edited and saved under another filename by clicking the Save As button Before the changed data takes effect the new filename needs to be loaded with the select lamp option described above CC UV VIS Fiber or integration sphere sample port diameter The hardware setup for which the calibration lamp has been calibrated CC UV VIS cosine corrector bare fiber or integrating sphere should match the hardware setup that is used during the intensity calibration The diameter of the cosine corrector 3900 micron bare fibe
196. with the right mouse button results in moving the complete spectrum up or down and to the left or right info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 76 more than spectroscopy Gel Spec com 3 a Exit m uai ow biegania iral o O fr mmo Faire je jp Fj IATE CE HH 02 a eee ee Ore Die a s e e s ee eee FHER TIMI AF sala Euminzn m Fiux imj 0 045 Coen B Hora getSoft IRRAD has been developed to perform on line absolute irradiance measurements with an getspec com s spectrometer system A calibrated light source getLight HAL CAL or getLight DH CAL with known energy output in uWatt cm nm is used as a reference Also the calibration of an getSpec com s spectrometer system can be done in the calibration lab after which this calibration can be loaded Color of light parameters can be expressed by the chromaticity coordinated x y and z These chromaticity coordinates are obtained by taking the ratios of the tristimulus values X Y and Z to their sum The tristimulus values X Y and Z and the spectral irradiance are computed in a wavelength range from 380 nm to 780 nm using a 1 nm interval These parameters as well as the coordinates u v and the color temperature of an external light source can be calculated and display
197. x 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 74 more than spectroscopy Gel specco m Enter Color Of Object settings Filename HE Load Color Ot Object settings Filename Opslaan als E data Ea c El Zoeken in E data J hahm Lj tst 32 i Ham256 x labE yellow coo Z leja Z smoothing Z smoothing speed zd speed time time Bestandsnaam den coo Bestandsnaam labE yellow coo Opslaan als type Color OF Object Settings Files Annuleren Bestandstypen Color Of Object settings Files Annuleren After the definition of one or more functions the OK button is clicked to start measuring the Cancel button to leave the dialog and return to getSoft s main window After starting the color measurements by clicking OK the output of the selected color parameters will be displayed against time If the option Display no graphics to speed up data processing has been marked in the function entry dialog the function output will be shown by numbers only which are updated each time a new scan is saved to the output file There are six buttons at the top of this window see figure below an Exit button a Pause Start button a Save Reference button a Save Dark button an Info button and a Print button Caim marii Fio If the red Exit button is clicked 25 a the time measurements are ended an
198. ximum around 14500 counts The easiest way to do this is by using the AutoConfigure Integration time option 4 Adjust the number of averages The higher the better however the time for a single absorbance reading must remain practical If you want to change it first press the Stop button and press the Start button again after changing the average value in the edit bar 5 Switch off the light source and save a dark spectrum switch it on again and save a reference 6 Change to absorbance mode by clicking the A button in the button bar Measure the absorbance of a sample with a high concentration to find the wavelength and bandwidth of an absorbance peak to use most of the time the wavelength with the highest absorbance is used 7 Calibrate your absorbance readings in the Chemometry Settings form section 4 6 2 You can also just use a previous calibration line as it can be saved to disk under an user defined filename and reloaded at the start of the application info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 107 more than spectroscopy 8 Enable the concentration measurements by selecting Application gt Chemometry gt Enable from the main menu After the application is enabled it is also possible to select Chem as a Hist
199. y omitting the settings dialog is clicking the LABChart button in getSofts main window The figure at the right shows all color IERI pene e UN parameters and all 3 charts were enabled mes neis seb UE The measurements in the figure are pones NEG OS cela ipie performed with the Master spectrometer H a5 0 06 channel and illuminant D65 has been selected The color parameters are calculated with the 2 degrees CIE standard observer values The filename to which data will be saved can be found in the lower left corner The button bar in the LABChart shows the following buttons o The Exit button is clicked to stop the color measurements and return to getSoft s main window o The Save Reference and Dark buttons have the same functionality as in getSoft s main window o The Save as reference color button is used to update the current reference color After clicking this button a dialog is displayed in which a name for the fa ProductlD already exists Biel Es ProductlD needs to be entered If the productID already exists in the The producti yellow tester database file and if the option Add The current color For this productlD in the database is new reference colors to DB when thee 5 48 mere 1089 bRER 7265 measuring is enabled in the LABChart settings dialog then a Do vou want to averwrite the color above by the new color below for this product dialog will be shown in which the ear
200. y come up which The file getUSB sys on getUSB allows you to browse to the directory _ Installation Disk is needed grice where the USB driver is located Click the Browse button and browse to the directory C AWINNT SYSTEM32 DRIVERS and double click the getUsb sys driver Then click the OK button the Files Browse Needed dialog at the right to complete the installation 7 The getSoft software can now be started and will detect the spectrometer at the USB port If not please have a look at the Device Manager which should contain the getSpec com Type the path where the file is located and then click OF Copy files from info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 114 more than spectroscopy Gel Spec com Spectrometer board under Universal Serial Bus controllers as illustrated in the figure above Do not hesitate to contact us if the problem remains unsolved info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 115
201. ze the comect operation of your 1y stem it will be the case for all drivers cithar immcdiataly ax in the luhan Micinsedt angy accemimerats Bal you sbop bee imatallak om now developed for Windows 2000 which contact the hadw n vendor lor seitan shat har i pecced w asceners Loge behing also work fine under Windows XP Select Continue Anyway Genius pny Depending on the Windows version the dialog at the right may come up which allows you to browse to the directory where the USB driver is located Click the Browse Rub E X button and browse to the directory The file getUSB sys on getUSB m C WINNT SYSTEM32 DRIVERS and P Disk a E double click the getUsb sys driver or for 7 __ Cancel the USB2 platform the get521 6USB sys Dp the path where the file iz located and then click driver If the computer does not support USB seres Windows 95 Windows NT 4 0 use a 3 pese standard RS 232 cable with male and female DB 9 connectors to connect the getSpec 2048 to the serial port of the computer info getSpec com Tel 49 0 351 871 8423 T l 33 0 4 72 80 82 02 Tel 49 0 231 974 2285 Fax 49 0 351 871 8465 Fax 33 0 4 72 80 82 43 Fax 49 0 231 974 2286 Dresden Lyon Dortmund Sofia Seite 6 more than spectroscopy get Spec COM 0 1 Bluetooth installation The getSpec spectrometers with Bluetooth wireless data transfer support are shipped with a Tornado

getSoft for getSpec–102/256/1024/2048 version 7.0 USER`S MANUAL

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getSoft for getSpec&ndash;102/256/1024/2048 version 7.0 USER`S MANUAL

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getSoft for getSpec–102/256/1024/2048 version 7.0 USER`S MANUAL