200001 how-to-use-Carlsberg-flask-Eng.
Contents
1. close the sample valve after the water has been expelled put the Carlsberg Flask in cold water or in a refrigerator for cooling disconnect the sterile air supply and the Teflon hose Method of transfer of the base substance to the Carlsberg Flask using 2 Keofitt valves close the sample valves on both the Carlsberg Flask and the container for the base substance connect the sample valves to the Teflon hose sterilise the connection by applying steam flow through the 2 valves fit an autoclave treated rubber cap onto the free connection piece on each valve open both valves to enable transfer of the base substance in order to provide sufficient pressure to enable the substance to run into the Carlsberg Flask sterile air can be supplied to a valve at the top of the container with base substance close both valves after transfer and disengage the Teflon hose Method of addition of culture of bacteria this can either be done by adding the culture of bacteria via the sample valve remember to sterilise first or by injecting via a Micro Port see extra fittings keofitt Oxidation close the sample valve sterilise the valve for 60 sec by applying steam at one of the connection pieces fit an autoclave treated rubber cap to one of the connection pieces supply sterile air to the other connection piece using an autoclave treated Quick coupling fitted to your sterile air hose set the pressure relief valve to the desired pressure the manomete2. User manual for Carlsberg Flask WORLD LEADERS IN STERILE SAMPLING keofitt KEOFITT CARLSBERG FLASK Dimensions The Keofitt Carlsberg Flask is produced in stainless steel and is supplied in Volume Diameter Height Net weight 25 270 mm 500 mm 19 kg Capacity Max 80 of the volume Standard fittings I manometer 0 2 5 bar 1 pressure relief valve 0 2 bar I sterile filter I m Teflon hose with 2 tube fittings I Quick coupling I Keofitt valve type W9 flanged to the center pipe I Micro port to allow the addition of culture or Spare parts bacteria Standard lid with 2 connection pieces O ring for lid 270 mm item no 200114 Manometer 0 2 5 bar item no 500101 Pressure relief valve 0 2 bar item no 900039 Sterile filter item no 200211 Filter for sterile filter item no 200210 Tube fitting item no 80007 1 Quick coupling item no 400086 Teflon hose 8 10 mm item no 900047 Keofitt valve type W9 CH item no 85002 1 600041 Membrane for valve type W9 item no 60005 I Rubber cap for valve type W9 item no 600062 Rustproof chain for valve type W9 item no 600064 Membrane for micro port Item no 900049 Keofitt a s knows from experience that the users of our Carlsberg Flask have their own methods and ways of using it Therefore the following should be regarded as our best suggestion as how to ensure the optimum sterile process Similarly Keofitt a s cannot provide instructions for the actual cultivation p
3. r has a maximum of 2 5 bar close the sample valve to enable sterile air to flow into the Carlsberg Flask close the valve after oxidation is complete disconnect the sterile air supply Method of transfer from Carlsberg Flask to container Follow the procedure described under Method of transfer of the base substance to Carlsberg Flask Sterile air can be supplied to the pressure relief valve in order to provide sufficient pressure to force the substance into the container Keofitt a s 19 11 1999
4. rocess 1 e quantities times temperature pressure etc We recommend a very thorough cleaning before use and all parts should be disassembled washed and treated in an autoclave before use keofitt Preparation of the Carlsberg Flask for sterilisation fit the O ring manometer and pressure relief valve to the lid fit the lid onto the Carlsberg Flask to avoid contamination it should not be removed during the process fit the membrane and the connection pieces to the sterile filter and fit it onto the pressure relief valve apply steam to the sterile filter so that steam flows through it and the pressure relief valve push the pipe with the sample taking valve down through the lid until it reaches the bottom of the Carlsberg Flask close the sample valve fit a rubber cap onto one of the connection pieces couple the Teflon hose onto the other connection piece Sterilisation of the Carlsberg Flask fill the Carlsberg Flask approx 75 with water and place it ready for boiling on an ordinary heating plate close to the outlet set the pressure relief valve to 2 bar boil the water for about 15 minutes connect the Teflon hose to the outlet the outlet must shall be lower than the Carlsberg Flask open the sample valve the pressure which has built up in the Carlsberg Flask will force the water out fit a Quick coupling to your sterile air hose and couple it to the sterile filter to force any remaining water out of the Carlsberg Flask
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