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O perating Instructions

1. 2 or A io aspirate 3 or w to dispense Each dispense is followed by an automatic blowout if enabled If the Mix option is on When a set number of cycles is selected mixing will execute automatically after each dispense followed by an automatic blowout if enabled Select PAUSE at any time fo pause mixing then RESUME to continue lf Manual is selected continue to hold w after dispense to execute mix cycle s Mixing will continue until the joystick is released Mixing is followed by an automatic blowout if enabled 4 ww or RESET for manual blowout s if needed Volume Sequencing Operation 1 4 to select the next preset series volume in the table if needed 2 or A io aspirate 3 or w to dispense Each dispense is followed by an automatic blowout if enabled The series number in the Volume Setting box will increment after each dispense If the Mix option is on When a set number of cycles is selected mixing will execute automatically after each dispense followed by an automatic blowout if enabled Select PAUSE at any time fo pause mixing then RESUME to continue 2 28 lf Manual is selected continue to hold w after dispense to execute mix cycle s Mixing will continue until the joystick is released Mixing is followed by an automatic blowout if enabled 4 w or RESET for manual blowouts if needed 5 Repeat the steps above until the series is complete Multi Dispense Mode Multi Dispense Mode pro
2. HU M3 Set of three individual magnetic Hang Ups for mounting on ferrous surfaces HU S3 Three Hang Ups attached to a clamp that fits onto a shelf Note All of the E4 XLS hangers listed above require a HU AS Adapter 1 19 Troubleshooting the Liquid End Problem Possible Cause Suggested Remedy Leaks Loose shaft Tighten coupling nut by hand inaccuracy Pipette volumes lt 2000 uL Replace seal and o ring worn seal or o ring pipettes worn o ring or insufficient grease seal 5000 uL 10 mL and 20 mL Replace o ring and apply small amount of grease Cracked or split shaft Replace shaft Check that piston is not bent If bent call Tech Sup port for a new piston 800 543 4030 in the US In other coun tries contact METTLER TOLEDO or your distributor Rough sample splash inside mech Remove tip ejector arm then remove shaft and check piston If jerky or anism leading to staining piston is corroded or stained call Tech Support for a new piston sticky and or corrosion 800 543 4030 in the US In other countries contact METTLER piston TOLEDO or your distributor When removing the shaft from the pipette body make sure the spring seal and o ring do not detach from the piston especially on smaller models Be careful not to bend the piston on these small models Recalibration of E4 XLS is only required when the piston is replaced Warning Messages E4 XLS monitors itself to inform y
3. TIP e y TIP Figure 16 Tip Immersion Depth and Tip Angle 1 6 4 Sleep Modes EA XLS is equipped with display and sleep timeout features both of which assist in conserving battery life sleep Mode reduces power consumption by 98 E4 XLS will automatically sleep after a set period To shut down the E4 XLS hold both buttons and ignore the warning message Display Timeout Dims the intensity of the display after a user specified period of inactivity To wake E4 XLS from a display timeout press either soft key or move the joystick in any direction Note The soft key or joystick action taken to exit a display timeout will return the E4 XLS to ready mode only The associated soft key function or joystick command will not initiate Sleep Timeout Powers the display off and puts the unit into low power state after a user specified period of inactivity To wake E4 XLS from a sleep timeout press either soft key The pipette will initialize and show the splash screen momentarily before returning the display to the last screen accessed Please refer to Setup Mode on page 34 for information on how to change the time setting for these timeout features Besides Sleep mode the E4 XLS can also shut down completely The E4 XLS automatically shuts down after 16 hours of non use If you wish to shut it down earlier push and hold both soft keys simultaneously After five seconds the units beeps twice and a warning screen notifies you
4. 3 or w to dispense If automatic blowout is enabled BLOWOUT will display in the operating screen w to execute blowout 4 ww or RESET for manual blowout s if needed Volume Sequencing Operation 1 4 to select the next preset series volume in the table if needed 2 or A io aspirate the preset volume plus blowout volume 3 or w to dispense The series number in the Volume Setting box will increment after each dispense If au tomatic blowout is enabled BLOWOUT will display in the operating screen wy to execute blowout 4 ww or RESET for manual blowout s if needed 5 Repeat steps above until the series is complete Dilute Mode Dilute Mode provides in tip dilution of multiple sample volumes Operation using the Single Vol ume setting allows aspiration of two volumes separated by an air gap Operation using the Vol 54 ume Sequencing option allows multiple volumes to be aspirated into the tip prior to dispense The 10 8 default menu level for Dilute Mode is Level II To access Dilute Mode 4 or p in the Level II Menu to highlight DILUTE and A or w io enter Options and Settings Options Volume Sequencing Mix Blowout Cycle Counter Settings Single Volume Volume Sequencing Multi Speed Mix Cycle Count Blowout Automatic and manual Options Selection 1 OPTIONS to enter the Options screen 2 or w io navigate between and highlight options 3 or to turn option on or off 4 DONE or 4 to s
5. gt 5000 pL Piston O Ring O Ring Shaft Tip Ejector Arm E4 5000 XLS 6200 131 6200 132 6202 063 6202 071 E4 10ML XLS 6200 138 6200 139 6202 064 6202 071 E4 20ML XLS 6200 143 6200 170 6202 065 6202 071 SE4 5000 XLS 6200 150 6200 151 6202 066 6202 073 SE4 10ML XLS 6200 154 6200 155 6202 067 6202 073 2 RFID Radio Frequency Identification Device 2 1 Description and Operation All E4 XLS models incorporate a passive RFID tag in the finger hook The tag contains unique information about the pipette including model type serial number RFID UID and service calibration information The RFID tag can be wirelessly linked with the optional reader and software to facilitate querying calibration tracking and service reminders The RFID tag is of negligible weight and has no effect on precision or accuracy lt operates only when read by an RFID reader and the associated software Optional Rainin RFID Reader Rainin recommends the Rainin RFID Reader which interfaces with a PC USB port and does not recommend any other RFID readers Using the Rainin RFID Reader information can be written to the pipette s RFID tag in the same consistent way as it is read from the RFID tag The Rainin RFID Reader requires LabX Direct Pipette Scan software for read write capability The estimated range of operation of the RFID reader is up to 5 cm M Rainin RFID Reader LED Indicators LEDs Description Yellow amp Green Reader powers up
6. The Sleep Timeout will power the display off after a period of inactivity in order to conserve battery life Time in tervals between 1 and 60 minutes can be selected Note If charging E4 XLS will not enter a sleep timeout if a cycle is incomplete for example when a sample has been aspirated but not yet dispensed To wake E4 XLS from a Sleep Timeout press either soft key If not charging E4 XLS will not enter a sleep timeout if a cycle is incomplete for an additional 15 minutes The inter rupted cycle is recoverable for up fo 18 hours when not charging 1 O or to edit 2 orp for coarse adjustment and 44 or w for fine 3 DONE or to save Time and Date Settings Available time and date settings are shown in Figure 19 SETUP 12 42 A Vi Time 00 42 19 Date 01 12 2010 Time Format 12 Hour Date Format dela AC HELP Figure 19 Setup Mode Page 2 of 4 Time shows the current time in a 24 hour HH MM SS format Time is displayed at the top right of all E4 XLS screens 1 or to edit 2 Hour will be selected initially 4 or gt to navigate between hour minute and second settings 3 A or w to change value 4 DONE or to save Date shows the current date using the current Date Format Setting 1 O or to edit 2 The first field will be selected initially 4 or to navigate between the other two date fields 3 or w to change value 4 DONE or io save 21 22 Time Display This is
7. USB to PC 3 Micro USB to wall charger DIMINISHING CAPACITY annual reduction in battery capacity INACTIVITY period of time with no user input POWER DOWN selectable to turn power off SLEEP automatic display goes blank power conservation mode after time out START unit activated by pressing either soft keys requires a single press TETHERED POWER OPERATION to use the unit while attached with a USB cable to a wall charger TIME OUT FOR SLEEP a user setting for duration before unit goes into sleep up to 60 minutes WAKE UP to restart full power mode from either sleep or deep sleep by pressing either soft keys requires a single press 6 62 5 3 Memory Power and Settings Management All user entered changes to settings on the E4 XLS as well as Service Mode data are saved in FLASH memory They are therefore preserved in the event of battery failure or removal with the exception of time and date see paragraph below If you wish to reset the pipette back to factory settings open the battery door Using a clean pipette tip gently press and hold the reset button see illustration below for about 7 seconds The unit will beep a splash screen will appear and a Memory Clear warning will be displayed Press the left softkey to continue This reset will NOT remove service data from the pipette RESET BUTTON Figure 38 Resetting E4 XLS The real time clock which keeps track of time and date requi
8. VERSION version number of the operating firmware GUI graphical user interface the displayed contents of the programmable features amp setting Ul user interface the total user experience combining the visual audio amp tactile features HELP mode user guide with detailed operating information LANGUAGE available languages that are programmed in the unit EN English ES Spanish FR French DE German ZH Chinese JA Japanese LIFETIME CYCLES total number of cycles since unit was manufactured MAIN menu the highest level in the programming sequence also called TOP menu MANUF DATE date unit was manufactured MINS minutes MENU a grouping of programmable features contained within a single screen MODE LEVELS user selectable level in the main menu for each mode MODEL NUMBER factory set volume range and tip style for each pipette NUMBER OF CYCLES number of cycles since the last service NUMBER OF DAYS number of days since the last service OFF option turned off ON option turned on OPTIONS additional settings available within a standard pipetting mode OWNER the operator or owner of the instrument PREV previous operating mode RESET to cancel cycle by moving the piston to blowout SAVE to enter into memory SECS seconds time measurement SETTING S optional pipetting inputs primarily volume and speed for a specific program SETUP optional inputs for customization by th
9. a faster rate To aspirate dispense smaller volumes at slower rates move the joystick slowly until the desired rate is achieved If the joystick is released operation will pause When joystick control resumes operation will continue from the same point 1 11 STEP will aspirate dispense the pipette s minimum allowable increment each time the soft key is pressed lf the value set in the Volume Setting is reached aspiration control will halt 3 When dispense is complete wy or RESET for manual blowouts if needed Volume Measurement Single or Fixed Volume Operation Single Volume operation skip to step 2 Fixed Volume operation 4 to select the next fixed volume in the table if needed 2 to aspirate the unknown volume of sample into the tip The amount of sample drawn into the tip is controlled directly through the joystick As the joystick is moved towards its maximum upward position larger volumes will be aspirated at a faster rate To aspirate smaller volumes at slower rates move the joystick slowly until the desired rate is achieved If the joystick is released operation will pause When joystick control resumes operation will continue from the same point STEP will aspirate the pipette s minimum allowable increment each time the soft key is pressed 3 If needed w slowly to release unwanted air from the tip 4 Ifthe value set in the Volume Setting is reached aspiration control will halt wy slowl
10. if it gets wet END OF END OF END OF 5000 pl SHAFT 10 mL SHAFT 20 mL SHAFT Ll j j O A i FILTER FILTER Figure 17 Filter Orientation 5000 uL and 10 mL Pipettes These models use the same filter as shown in Figure 17 5000 uL insert the small diameter into the shaft 10 mL insert the large diameter into the shaft Filter part numbers 6190 164 pack of 100 6190 165 pack of 1000 20 mL Pipettes The filter for these models is a cylinder Part numbers 6190 221 pack of 100 6190 222 pack of 500 1 7 E4 XLS Volume Ranges and Increments Volume ranges and increments for each E4 XLS model are shown in the following tables Single Channel E4 XLS Models Adjustable Recommended Increment Volume pl Range pl Range pl pL 10 O to 10 0 5 to 10 0 01 20 O to 20 2 to 20 0 02 100 O to 100 10 to 100 0 1 200 O to 200 20 to 200 0 2 300 O to 300 20 to 300 0 2 1000 O to 1000 100 to 1000 1 2000 O to 2000 200 to 2000 2 5000 O to 5000 500 to 5000 5 10 ml Oto 10 ml 1 to 10 mL 10 20 ml O to 20 ml 2 to 20 mL 20 Multichannel and Adjustable Spacer Models Adjustable Recommended Increment Volume pl Range ul Range pl pl 10 Oto 10 0 5 to 10 0 01 20 O to 20 2 to 20 0 02 50 O to 50 5 to 50 0 05 100 O to 100 10 to 100 0 1 200 O to 200 20 to 200 0 2 300 O to 300 20 to 300 0 2 1200 O to 1200 100 to 1200 1 1 8 E4 XLS Sound Alerts General Soun
11. in the upper left of each screen HELP for a detailed description of the highlighted item MAIN fo return to the Main Menu 20 Sound and Display Settings Available sound display and timeout settings are shown in Figure 18 SETUP E sound Display 10 Brightness Display Timeout 1S Sees Sleep Timeout 9 Mins MAIN 42P HELP Figure 18 Setup Mode Page 1 of 4 Sound Level This is the volume setting for E4 XLS audible activity alerts Volume levels between 1 and 10 or Off can be se lected 1 orp to edit 2 dor gt for coarse adjustment of Off 5 or 10 and A or w for fine adjustment in single digits w or q at 1 for Off 3 DONE or to save Display Brightness This is the light intensity setting for the E4 XLS display Values between 1 and 10 can be selected where is the dimmest and 10 is the brightest setting 1 orp to edit 2 4 or gt for coarse adjustment and A or w for fine The screen brightness will automatically adjust as the setting is changed 3 DONE or to save Display Timeout The Display Timeout will dim the display after a period of inactivity in order to conserve battery life Time inter vals between 5 and 120 seconds or Never can be selected Note To wake E4 XLS from a Display Timeout press either soft key or move the joystick in any direction 1 orp to edit 2 4 or b for coarse adjustment and or w for fine 3 DONE or to save Sleep Timeout
12. in this state and reverts to this State when the application is stopped or a plug in is disabled Yellow Reader has detected a pipette Keep pipette close to reader until LED changes to Green or Red Green When the application first starts the Green LED indicates the device is ready After all data is read from a pipette the Green LED lights up Red Reader failed to read or write Try scanning the pipette again If the LED stays red restart the soft ware Yellow amp Red Data written to the pipette does not match the data read back from the pipette Optional LabX Direct Pipette Scan Software supplied on the same CD as this manual is a trial version of the LabX Direct Pipette Scan software A full ver sion is also available for purchase Supported PC Operating Systems include Microsoft Windows XP Vista and Windows 7 Users can select any of the following languages Chinese Simplified Czech Danish English French German Hungarian Italian Japanese Korean Norwegian Polish Russian Spanish and Swedish 5 52 Operation Overview The optional RFID Kit contains a Rainin RFID Reader and LabX Direct Pipette scan software Once the Rainin RFID Reader is connected via USB to a PC and the software is configured and running operation is simple Place the head of the pipette over the Rainin RFID reader in the position shown in Figure 30 Hold the pipette steady for a few seconds fo allow the pip
13. mix speeds DILUTE mode fo pick up two or more samples which may be separated by an air gap DISPENSE to release an amount of liquid DISP SPEED dispense speed DONE a single keystroke that saves setting and in some cases exits to prior screen END VOLUMES final selection from a list of preset volumes FAST DISP VOL in titrate mode the first volume dispensed before fine increment dispensing FIXED VOLUMES FIXED VOL to move a consistent set of fixed aspiration dispensing steps without variation same volume all the time GLP mode good laboratory practices tracks the service and calibration of lab instruments HOME piston position after returning from blow out ready to aspirate LEVEL within the main menu the first set of available modes LEVEL II within the main menu the second set of available modes MANUAL mode dynamic control of the motor to move the piston up or down using the joystick MIX to move the pipette s piston up and down a number of times to mix sample MODE a distinct subset of the operation of the pipette MULTI DISPENSE MULTI DISP mode to dispense multiple aliquots from one pick up volume BASIC mode to pick up and dispense a known volume PIPETTE amp MIX mode to pick up and dispense a known volume into another sample then mixing by rapidly aspirating amp dispensing in and out of the tip REVERSE mode method of picking up the blow out phase or second stop and dispensing on
14. need to be fully discharged Pipettes can be charged using the Wall Power Supply the optional Rapid Charge Stand or with a PC using the optional USB cable The battery charge indicator in the top right corner of the display will change as the unit is charging After 15 minutes the pipette will have enough charge for a few hours of pipetting and a full charge will be obtained after approximately 90 minutes and will provide up to 3000 full stroke cycles fewer in large volume models When charging is complete the battery charge indicator will be solid green indicating a full battery As the bat tery depletes with use the green bar will drop accordingly 43 44 Wall Power Supply To install the universal Wall Power Supply and charge E4 XLS connect the power cord to a power outlet Con nect the other end of the cord to the Micro USB port located on the top of E4 XLS E4 XLS can be operated while connected to the Wall Power Supply cord Figure 26 Operating E4 XLS while connected to E4 WPS Wall Power Supply Rapid Charge Stand To use connect the universal Rapid Charge Stand power cord to a power outlet and connect the other end of the cord to the power socket on the back of the Rapid Charge Stand For convenience you can dress the cable along one of the vertical support legs using the channels provided See Figure 27 B Figure 27 A E4 RCS holding E4 XLS pipettes B Back of E4 RCS showing power cord connected and dre
15. senna 15 LOS TIMES Dela A ias 16 0 ISE MOOS ran da ie 16 FOG Ol Dos cada 17 ESO Mr dai 17 1 7 EA XLS Volume Ranges and Incio me aserrada 18 WSO EAS OGUNGA Seta area sta 18 EO SOU HVIO GG AMA e ro IS 19 IO EA XLS ODE rational NOS AR ia 19 LA Lever tna ria dla 33 liz Servico MONO caca old cosa 33 Waly HOMO A A A aha ete mates desaes 4 1514 ISG DIGCHG IMO BANOT dad 45 LIS Care ana NENA Crit alias 46 MTs SMUG LO LEN A eatin ene 46 MAG TIDSE CCIOR AN A O O echo aaa 46 A iaitre te once dial tui taageeet sigs Aniodt haw scien ai Aa a T A 47 Sk Troubleshooting Me Liguid ri a a a A uddcameseuannes 47 1 20 Service Calibration and REpaliF oooccooccnncnnoccnoncnnnconorononononcnnononononnncnnoronononononononnnnrnnononoss 48 SpPECINCANONS sormea a ib 49 t22 REDUCEN nana 50 2 RFID Radio Frequency Identification Device oo00coooccoocccooocccococononnnnnrnnnononnnos 51 Zi ADesScupioMana Pe Orar J 3 EA XLS M ltichannel PIDOTTOS nccrne aE ica 54 Sek Descuipiomand Operaio risina ion a T 54 4 E4 XLS Multichannel Pipette with Adjustable Spacing annn 56 4 1 Description and UM ri oa poor odos 56 5 Appendices to be added by Rainin oooooccocococccccococcconoocconnoooconnonconcnnoniononnnnnonons 59 5k CCG Oli aaa 59 dez GOSSA ONTOS aa 60 5 3 Memory Power and Settings Management cccoooccnccoccnnccoconococonnnonononcninnnnncnnon
16. that you are about to shut down the unit Keep holding the buttons and the unit will shut down five seconds later To turn on unit press any soft key 1 6 5 1 6 6 Help On screen Help is available to assist users with step by step operation and provide detailed information for a particular screen menu ifem setting or option Menu Help To get more information on a specific carousel menu item highlight the item and select HELP Settings and Options Help To get more information on a specific setting or option in the current screen high light the item and select HELP Note For all advanced operational modes operating instructions can be found in the Help for the first setting in the mode s operating screen General Information In some screens general information and or operating instructions can be accessed directly without having to highlight a particular item In these screens HELP will be an available function as soon as the screen is accessed Note In Basic Mode only operating instructions can be found in the general help for the mode s operating screen select RETURN fo exit Help and return fo the previous screen Filter E4 XLS 5000 uL 10 mL and 20 mL pipettes use a filter in the end of the shaft This helps prevent liquid from entering the shaft and contaminating the piston should the plunger snap up during aspiration Using a filter is recommended when pipetting large volumes The filter should be replaced
17. the Wall Power Supply and Rapid Charge Stand can quickly be disconnected from AC power in case a hazard arises Rapid Charge Stand Input E4 RCS 100 240 VAC 50 60 Hz 3A Wall Power Supply Input E4 WPS 100 240 VAC 50 60 Hz 1 2A Batiery 6109 030 Li lon 3 6 VDC Nominal 1100 mAh Nominal Explanation of symbols Hz Hertz A Aamperes mAh Milliamp Hour VAC Volts Alternating Current VDC Volts Direct Current 1 22 Replacement Parts The commonly replaced parts are shown here for each volume range for E4 XLS single channel LTS and 50 universal fit models Models lt 2000 pL Seal O Ring Shaft Tip Ejector Arm E4 10 XLS 6200 138 6200 139 6202 064 6202 071 E4 20 XLS 6200 143 6200 170 6202 065 6202 071 E4 100 XLS 6200 150 6200 151 6202 066 6202 073 E4 200 XLS 6200 154 6200 155 6202 067 6202 073 E4 300 XLS 6200 415 6200 414 6202 425 6202 419 E4 1000 XLS 6200 161 6200 162 6202 068 6202 074 E4 2000 XLS 6200 166 6200 167 6202 214 6200 168 SE4 2 XLS 6200 131 6200 132 6200 134 6200 133 SE4 10 XLS 6200 138 6200 139 6200 140 6200 133 SE4 20 XLS 6200 143 6200 170 6200 145 6200 144 SE4 100 XLS 6200 150 6200 151 6200 147 6200 148 SE4 200 XLS 6200 154 6200 155 6200 157 6200 156 SE4 300 XLS 6200 415 6200 414 6200 413 6200 419 SE4 1000 XLS 6200 161 6200 162 6200 160 6200 163 SE4 2000 XLS 6200 166 6200 167 6200 169 6200 168 Cylinder Models
18. to edit the Speed Setting The settings box will turn white to indicate it is in edit mode Figure 10 right In Basic Mode the same speed is used for both aspirate and dispense 4 or p for coarse speed ad justment of 1 5 or 10 and Am or w for fine adjustment in single digits Hold the joystick in place to quickly scroll through values 25251 il 200 0uL 200 0uL Volume Volume Speed Speed DONE sh HELP DONE 48 gt CANCEL Figure 10 Basic Mode with Speed Highlighted left and Speed in Edit Mode right 7 DONE or to save the Speed Setting CANCEL to exit without saving changes 8 DONE to exit the Settings screen and return to the operating screen If Basic Mode is exited all settings are retained when the mode is accessed again Aspiration The mode operation will flash ASPIRATE indicating E4 XLS is ready fo aspirate sample The joystick controls used for aspiration will also flash in the navigation icon Figure 11 MAIN Ll HELP Figure 11 Basic Mode Aspirate Operation 1 Attach a new Rainin tip Press the shaft into the tip with only enough force to make a good seal 2 Hold E4 XLS vertically or within 20 degrees of vertical Place the tip into the sample at the recommended im mersion depth see section 1 6 3 on page 16 3 or A to aspirate the sample lt is not necessary to hold the joystick in place E4 XLS will automatically complete the operation The Tip Volume Indicator will mimic sample aspi
19. A8 Nozzle spacing adjustment ranges are listed below 6 channel 9 mm to approximately 19 mm 8 channel 9 mm to approximately 14 mm 6 channel versions are available in the following volume ranges 20 300 yL 100 1200 uL 8 channel versions are available in the following volume ranges 5 50 uL 20 300 uL 100 1200 uL Spacing Controls and Indicators Nozzle spacing is changed with the use of two knurled knobs on either end of the liquid end manifold the SPACING ADJUSTMENT knob and the LIMITER knob shown in Figure 34 The LIMITER knob which is marked with the nozzle spacing range sets the desired maximum spacing within the pipette s spacing range A verti cal arrow at the top of the knob on the liquid end manifold is the set point shown in Figure 35 When the maximum spacing with the LIMITER knob has been set turn the SPACING adjustment knob to open the nozzle spacing to the set value A scale on the liquid end manifold gives a visual reference of the set spac ing Simply line up the leftmost nozzle with the scale on the manifold The full range of adjustment for the 8 channel version is shown in Figure 36 Nozzles are set to 9 mm spacing on the left and 14 mm spacing on the right O A Figure 34 E4 XLS Adjustable Spacer A SPACING ADJUSTMENT B LIMITER SET POINT RAININ Figure 35 Limiter Set Point E os Figure 36 Nozzles Minimum Spacing left and Maximum Spacing right Till s
20. DONE or to save Mode Levels The operational modes available for selection in either the Level or Level Il Menu SETUP 2 34PM DE can be customized by the user Mode access can be moved between menu levels as e Mode Level needed Once a mode s menu level is changed it will be available only in the menu it BASIC LEVEL dt ADVANCED LEYEL I was assigned to MULTEDISP LEYEL MAHUAL LEVEL REVERSE LEVEL Il DILUTE LEVEL Il TITRATE LEVEL Il SETUP LEVEL 1 or to view the mode menu table or w io highlight a mode s level io edit or w io select Level or Level II Menu DONE or to save O a BRB U N DONE fo exit table Modes will immediately display as menu items in the selected menu Alarms Users can set service alarms based on number of cycles executed or days in use Alarm options and settings are shown in Figure 21 SETUP Cycle Alarm i Number Of Cycles Ll Days Alarm On Number Of MAIN 4 HELP Figure 21 Setup Mode Page 4 of 4 Cycle Alarm The Cycle Alarm will notify users that pipette service is due after a set number of aspirate dispense cycles have been executed To set a Cycle Alarm 1 dor to turn the Cycle Alarm on 2 ww to highlight the Number of Cycles Setting 3 or to edit 23 4 dor for coarse adjustment and 44 or w for fine adjustment Values between 1000 and 1 000 000 can be set 5 DONE to save changes When the value set in th
21. LEDO Company www mt com rainin For more information
22. NE to exit Settings screen and return to operating screen Volume Sequencing Vol X of X is displayed in Volume Setting box Option must be on for this setting to be shown or to view the volume table l E Volumes ul 1 2 10 0 3 20 0 4 5 To select a series of preset volumes use the joystick to highlight the last volume for the series Only active table cells white text can be selected END VOL to set DONE to exit table 25 0 30 0 2 To edit a preset volume use the joystick to highlight the volume or to edit Both active and inactive cells can be edited q or gt for coarse adjustment and 4 or y for fine DONE or to save Repeat as needed for other volumes CANCEL to exit without saving changes DONE to exit table Note If an END VOL is not set before exiting the table the END VOL last selected will be retained 3 DONE to exit Settings screen and return to operating screen Speed 1 or to view individual operation speeds Cycle Speeds Note The mix operation speed is shown only when the Mix option is on Aspirate 2 or w to highlight setting or gt to edit Dispense 10 Mi 5 3 or for coarse speed adjustment of 1 5 or 10 and A or w for fine adjustment in la single digits 4 DONE or to save CANCEL to exit without saving changes DONE to exit table 5 DONE to exit Settings screen and return to operating screen Mix Mix Settings Option must be on for this setting to be shown or p t
23. RAININ Pipetting 360 E4 XLS Electronic Pipettes Advanced Electronic Pipette with RFID Y po a ESS QO as Y Sj O U aD O O E4 XLS Electronic Pipettes e Single channel models with LTS or with traditional universal fit shafts e Multichannel models with LTS e Adjustable spacer multichannel models with LTS e All models RFID enabled Contents of E4 XLS Box EA XLS Electronic Pipette Battery Quick Reference Guide CD containing this manual and trial REID software sample Tips Wall Power Supply Conformance Certificate and Warranty Card The optional E4 XLS USB cable and EAXLS Rapid Charge Stand are available separately If any item is missing please call 800 472 4646 in the US or contact your local METTLER TOLEDO office or distributor safety Notice If this product is used other than as described in this manual its safety protection may be impaired Rainin XLS LTS LiteTouch Hang Ups are trademarks of Rainin Instrument LLC 2011 Rainin Instrument LLC No part of this manual may be copied without the express written permission of Rainin Instrument LLC Contents 1 E4 ALS ELCCTIONIC PIDOTOS caeci Si a 4 A a A A 4 L2 EAS PIDO ONO Wa ipods 4 ESA E O O tants 6 ras MENUS ANA MIOS asadas 11 WO OPMONS and SAS dde 12 16 USINO Ihe PIDENG si ld 15 OT PIDGMINGHGUIDCIINGS irune a aaa a o a A 15 162 Mp Seiecion and MOLA irse senarteea
24. TTLER TOLEDO or your distributor 1 20Service Calibration and Repair It is recommended to use only genuine Rainin replacement parts such as seals o rings and shafts It is NOT necessary to recalibrate the pipette after changing the seal o ring or shaft Recalibration of the pipette is only necessary when the piston is replaced and should be done only by qualified factory trained personnel in a Rainin approved facility For pipettes under warranty please note that the warranty will be voided if the pipette has been damaged as a result of physical or chemical abuse or if the pipette has been repaired or recalibrated by any service facility which is not authorized by Rainin In the US call 800 543 4030 for service Service is also available outside the US See www mt com rainin for more information Acids and Corrosives Extensive contact with corrosive fumes may result in premature seal wear and damage to the piston Exposure of the internal components to corrosive aerosols and fumes may be reduced by using Rainin tips with aerosol barrier filters After using concentrated acids or corrosive solutions disassemble E4 XLS and inspect and clean the piston as sembly shaft and seal o ring with distilled water Use extreme care on the 10 uL models to avoid damaging the small diameter piston or losing small items such as seals Dry all components thoroughly and reassemble 1 21 Specifications These manufacturer s specifications should b
25. Te fpe Tip Selection E4 XLS multichannel pipettes use Rainin LTS tips Use 250 uL LTS tips with the EA BOXLS Use 300 uL LTS tips with the EA6 300XLS and EA8 300XLS 1200 uL LTS tips are recommended for the EA6 1200XLS and EA8 1200XLS Non filter 1000 uL LTS tips can also be used as they will pick up 1200 UL LTS tips have a cylindrical cross section with a well defined seal ring thin wall and positive stop They seal properly on the LTS nozzles and cannot be jammed or forced too far onto the nozzles Mounting LTS Tips Mounting racked LTS tips on Adjustable Spacer XLS pipettes is simple 1 Set the nozzle spacing to 9 mm same as the spacing for racked tips by rotating the dark grey SPACING AD JUSTMENT knob fully clockwise 2 Align the nozzles into the row of tips holding the pipette at a slight angle Figure 37 left 3 Rotate the pipette upright and press the nozzles into the tips until the positive stop is reached The tips are now mounted with proper sealing There is no need to hand tighten use heavy pressure or rock the nozzles onto the tips to obtain a good seal Withdraw the tips from the tip rack Figure 37 right Figure 37 Mounting LTS Tips 57 58 Setting the Nozzle Spacing With tips mounted on the nozzles and before aspirating sample lf the spacing for the receiving wells or test tube block is known set this value on the LIMITER knob lf fhe spacing is not known hold the pipett
26. Volume Volume is displayed in Volume Setting box This value represents the maximum aspiration volume required for the desired measurement 1 O orp to edit 2 or for coarse adjustment and or w for fine 3 DONE or to save CANCEL fo exit without saving changes 4 DONE fo exit Settings screen and return to operating screen Fixed Volume Fixed Vol X is displayed in Volume Setting box Option must be on for this setting to be shown This value repre sents the maximum aspiration volume required for the desired measurement or gt to view the volume table The volume currently selected will be highlighted in the table 1 To select a preset volume use the joystick to highlight a volume Only active table cells white text can be selected DONE to set and exit table 3 32 2 To add or remove active cells in the table use the joystick to highlight a cell that represents the last entry needed END VOL to add cells up to or remove cells after the highlighted cell To edit a preset volume use the joystick to highlight the volume or to edit Both active and inactive cells can be edited 4 or for coarse adjustment and or w for fine DONE or to save Repeat as needed for other volumes CANCEL to exit without saving changes DONE to exit table Note If no other selection is made before exiting the table the Volume Setting will be set to the last volume edited 4 DONE to exit Settings screen and return to oper
27. able use the joystick to highlight a cell that represents the last entry needed END VOL to add cells up to or remove cells after the highlighted cell 3 To edit a preset volume use the joystick to highlight the volume or to edit Both active and inactive cells can be edited 4 or for coarse adjustment and or w for fine DONE or to save Repeat as needed for other volumes CANCEL to exit without saving changes DONE to exit table Note If no other selection is made before exiting the table the Volume Setting will be set to the last volume edited 4 DONE to exit Settings screen and return to operating screen Volume Sequencing Vol X of X is displayed in Volume Setting box Option must be on for this setting to be shown or gt to view the volume table Too ul 10 0 1 To select a series of preset volumes use the joystick to highlight the last volume for 20 0 the series Only active table cells white text can be selected END VOL to set DONE IET fo exit table she 2 To edit a preset volume use the joystick to highlight the volume or gt to edit Both active and inactive cells can be edited 4 or for coarse adjustment and 44 or y for fine DONE or to save Repeat as needed for other volumes CANCEL to exit without saving changes DONE to exit table Note If an END VOL is not set before exiting the table the END VOL last selected will be retained 3 DONE to exit Settings screen and return to o
28. aining titration volume Level Menu The Level Menu Figure 14 is used to select some E4 XLS operational modes and access the Level Il Menu Please refer to 1 9 Level II Menu on page 33 for more information on this menu The default Level Menu operational mode selections include Basic Mode Advanced Mode Multi Dispense Mode Manual Mode and Setup Mode Menu Navigation In this manual we use the convention of showing the image of the con trol fo denote the action of selecting that control As examples the gt key means move the joystick fo the right OPTIONS means Select the OPTIONS soft key orp to view and select menu items A or w io enter the selected Basic Mode or Level Il Menu PREV Returns to the previously used mode allows toggling between modes HELP for a detailed description of the highlighted menu item SETUP to go directly to Setup Mode and change pipette settings Figure 14 E4 XLS Controls 15 Options and Settings The list of common options and settings is provided in the table below A detailed description of these and other mode specific options follows Single Fixed Volume Single Multi Mix Auto Cycle Auto Volume Volume Sequencing Speed Speed Blowout Counter Pace Basic E al Advanced E E m E a E m Multi dispense E m E E Manual E E m E Reverse E E E E E E Dilute E E El E E E Titrate E E E Speed
29. al blowouts if needed Service Mode O Service Mode allows users to view information specific to E4 XLS such as the serial number GLP E Y firmware version and the full service log In addition usage logs including the number of pi petting cycles or days in use can be viewed and tracked for GLP purposes To access Service Sambil Mode 4 or gt in the Level Il Menu to highlight SERVICE and A or w to enter Navigation of Service Pages To move between pages gt or w for the next page and 4 or for the previous page The current page will be displayed in the upper left of each screen HELP for a detailed description of the highlighted item or page MAIN to return to the Level ll Menu Identification Page This page contains pipette specific identification details Figure 23 This information is entered at the time of manufacture and is not user settable Serial Number Manufacture Date Model Number Firmware Version Note When new firmware is downloaded to E4 XLS the firmware version number displayed on the identification page will update automatically 4 42 SERVICE 5 46 PM al Serial ik 12345678 Manuf Date 05 09 10 Model Number 200uI LTS Firmware Wareian 1 0 34 498 MAIN 42P HELP Figure 23 Identification Page The following icons can be displayed on this service page cy Indicates a Service Alarm has been activated E Indicates an optional microSD card is ins
30. ating screen Speed 1 2 3 4 O or p to edit 4 or gt for coarse speed adjustment of 1 5 or 10 and Am or w for fine adjustment in single digits DONE or O to save CANCEL to exit without saving changes DONE to exit Settings screen and return to operating screen Cycle Count Option must be on for this setting to be shown or to edit RESET to zero counter 4 or for coarse adjustment and 44 or w for fine DONE or to save CANCEL to exit without saving changes DONE to exit Settings screen and return to operating screen Operation The active operation will be displayed in the upper left of the operating screen At any time during operation RESET empties the tip and resets the pipette operation to ASPIRATE MAIN displays the menu the mode was accessed from HELP provides general information for the highlighted setting Operating instructions can be found in the Help for the Volume Setting Single and Fixed Volume Operation l 2 single Volume operation skip to step 2 Fixed Volume operation d to select the next fixed volume in the table if needed and hold to aspirate then wy and hold fo dispense at any time It is not necessary to aspirate the full amount in the Volume Setting before beginning dispense The amount of sample aspirated or dispense is controlled directly through the joystick As the joystick is moved towards its maximum position larger volumes will aspirate dispense at
31. ave and return to the operating screen Editing Settings When editing settings hold the joystick in place to quickly scroll through values 1 p to enter the Settings screen 2 A or w io navigate between and highlight settings Single Volume Volume X is displayed in Volume Setting box The Single Volume setting allows two dilution volumes to be set for aspiration During operation Asp 1 will be aspirated first followed by an air gap then Asp 2 Note Rainin recommends using Asp 1 for diluent and Asp 2 for sample 1 or gt to view Dilute Volumes 41 or w to highlight setting Dilute Volumes 2 orp to edit asp 80 0 Asp a 20 0 3 or p for coarse adjustment and 44 or w for fine Note Selectable Asp 2 values will automatically adjust to only allow values that will not exceed the pipette maximum once the Asp volume is aspirated 4 DONE or O to save CANCEL to exit without saving changes DONE to exit fable 5 DONE to exit Settings screen and return to operating screen Volume Sequencing Vol X of X is displayed in Volume Setting box Option must be on for this setting to be Seg Volumes ul shown During operation each volume selected will be aspirated in series or to view the volume BEET T 20 0 1 To select a series of preset volumes use the joystick to highlight the last volume for 4 25 0 the series Only active table cells white text can be selected END VOL to set DONE Eiig to exit tabl
32. aximum pipette volume with the current Aliquot Volume Setting If the desired value cannot be selected change the Aliquot Volume setting to a lower value The Volume Setting will automatically adjust based on the new Aliquots Setting and the existing Aliquot Volume Setting 2 If needed set the aliquot volume by highlighting the Aliquot Volume Setting box Aliquot Vol or gt to edit q or gt for coarse adjustment and A or w for fine DONE or to save CANCEL fo exit without saving changes Note E4 XLS will only allow values in the Aliquot Volume Setting that will not exceed the maximum pipette volume with the current Aliquots Setting The Volume Setting will automatically adjust based on the new Ali quot Volume Setting and the existing Aliquots Setting 3 DONE to exit Settings screen and return to operating screen Single Volume Dispensing Based on Aliquot Volume 1 Highlight the Aliquot Volume Setting box Aliquot Vol or gt to edit 4 or gt for coarse adjustment and 4 or w for fine DONE or to save CANCEL to exit without saving changes Note E4 XLS will only allow values in the Aliquot Volume Setting that will not exceed the maximum pipette volume with the current Aliquots Setting If the desired value cannot be selected change the Aliquots Setting to a lower value The Volume Setting will automatically adjust based on the new Aliquot Volume Setting and the existing Aliquots Setting 2 If needed set the numb
33. c Mode on page 7 Detailed settings and options descriptions can be found in Options and Settings on page 7 This section will provide mode specific information and operating instructions for each of the E4 XLS operational modes 24 Basic Mode Basic Mode provides basic laboratory pipetting needs Users can select a single volume and one speed that is used for both aspirate and dispense The default menu level for Basic Mode is Level To access Basic Mode 4 or in the Main Menu to highlight BASIC and A or w to enter Options and Settings Options None settings Single Volume Single Speed Blowout Automatic and manually repeatable Advanced Mode Advanced Mode provides the complete range of all E4 XLS advanced pipetting options The de fault menu level for Advanced Mode is Level 1 To access Advanced Mode q or gt in the Main Menu to highlight ADVANCED and A or w io enter ADVANCED Options and Settings Options Fixed Volume Volume Sequencing Mix Blowout Cycle Counter Settings Single and Fixed Volume Volume Sequencing Multi Speed Mix Cycle Count Blowout Aufomatic and manual Options Selection 1 OPTIONS to enter the Options screen 2 or w to navigate between and highlight options 4 or w at the first or last option on a page to navi gate between pages 3 or to turn option on or off 4 DONE or 4 to save and return to the operating screen Editing Settings When e
34. d Alerts Aspiration complete High tone ding Dispense complete Mid tone ding Blowout complete Low tone ding Reset activated Two high tone beeps Reset complete piston in home position Low tone ding Soft key function accessed Short click 1 9 Menu navigation Swoosh Menu item selected Short click setting or option selection Short click setting value or option edit Short click Setting error min max setting has been reached Buzz Cancel Swoosh Low battery warning Two high tone beeps service Alarm Two high tone beeps Mode Specific Sound Alerts Multi Dispense Mode A second mid tone ding will sound after the last aliquot is dispensed Manual Mode Aspirate and dispense complete alerts will sound only when the value set in the Volume Setting is fully aspirated or dispensed Titrate Mode After Fast Dispense the dispense complete alert will sound again only if the value set in the Vol ume Setting is fully dispensed Setup Mode setup Mode allows users to personalize general pipette settings User settable service alarms can also be acti vated The default menu level for Setup Mode is Level To access Setup Mode 4 or gt in the Main Menu to high light SETUP and A or w io enter Navigation of Setup Pages or w to move between and highlight a setting To move between pages or w at the first or last setting on a page or 4 at any time The current page number is displayed
35. diting settings hold the joystick in place to quickly scroll through values 1 gt to enter the Settings screen 2 A or w io navigate between and highlight settings Single Volume Volume is displayed in the Volume Setting box orp to edit orp for coarse adjustment and 44 or w for fine DONE or to save CANCEL to exit without saving changes gt WON DONE to exit Settings screen and return to operating screen 25 26 Fixed Volume Fixed Vol is displayed in Volume Setting box Option must be on for this setting to be Fixed Yolumes ul shown O or gt to view the volume table The volume currently selected will be high lighted in the table To select a preset volume use the joystick to highlight a volume Only active table cells white text can be selected DONE to set and exit table To add or remove active cells in the table use the joystick to highlight a cell that represents the last entry needed END VOL to add cells up to or remove cells after the highlighted cell To edit a preset volume use the joystick to highlight the volume or to edit Both active and inactive cells can be edited 4 or gt for coarse adjustment and 4 or w for fine DONE or fo save Repeat as needed for other volumes CANCEL to exit without saving changes DONE to exit table Note If no other selection is made before exiting the table the Volume Setting will be set to the last volume edited DO
36. e Note E4 XLS will not allow selection of an END VOL that will cause the total series volume to exceed the pipette maximum If this occurs edit the desired END VOL value until the total series volume is less than the pipette max volume 2 To edit a preset volume use the joystick to highlight the volume or gt to edit Both active and inactive cells can be edited 4 or gt for coarse adjustment and or w for fine DONE or to save Repeat as needed for other volumes CANCEL to exit without saving changes DONE to exit table Note Preset volumes can be used for samples diluents and air gaps If an END VOL is not set before exiting the table the END VOL last selected will be retained 3 DONE to exit Settings screen and return to operating screen Speed 1 or gt to view individual operation speeds Cycle Speeds Note The mix operation speed is shown only when the Mix option is on Aspirate 2 or w to highlight setting or p to edit Dispense 10 Mix 5 3 or for coarse speed adjustment of 1 5 or 10 and or w for fine adjustment in single digits 4 DONE or to save CANCEL to exit without saving changes DONE to exit fable 5 DONE to exit Settings screen and return to operating screen 37 38 Mix Option must be on for this setting to be shown or p to view mix settings 4 or w lo sn a Mix Settings highlight setting volume 40 0ul 1 To change volume or p to edit 4 or lt p f
37. e Blowout Operation 4 Press the tip ejector button lightly to discard the tip To prevent carry over use a new tip for each sample 1 4 Menus and Modes E4 XLS utilizes two menu levels that allow user selection of pipetting modes and provide access to general pi pette information and settings Level Menu Allows selection of common operational modes and access to the Level II Menu Level Il Menu Provides selection of additional modes pipette and personalization settings service informa tion and options and access fo Level menu The following operational modes offer a wide variety of pipetting capabilities Basic Mode Basic pipetting operation Selection of a single volume and one aspirate dispense speed Advanced Mode Provides all E4 XLS advanced pipetting options such as Mix and Volume Sequencing Multi Dispense Mode Provides multi aliquot dispensing with user settable automatic dispense intervals Manual Mode Provides joystick control of E4 XLS that simulates manual pipette operation Fine control of pre cise volume increments allows aspiration and dispense of measured sample volumes Reverse Mode Aspirates the selected volume along with the pipette blowout volume Recommended for dense or volatile liquids Dilute Mode Provides in tip dilution of multiple sample volumes Titrate Mode Performs titration through measured dispensing Allows an initial rapid dispense followed by precise control of the rem
38. e Number of Cycles Setting has been reached E4 XLS will present the following An on screen Service Alarm notification E Service Alarm 10 000 Cycles have elapsed since The Service Alarm icon will display in the Service Mode Identification Page last service Note To exit a notification message select CONT These notifications will continue to display until the Cycle Alarm is turned off the Number of Cycles Setting is reset or service is performed Days Alarm The Days Alarm will notify users that pipette service is due after the pipette has been in use for a set number of days To set a Days Alarm 1 0 dor to turn the Days Alarm on 2 ww to highlight the Number of Days Setting 3 orp to edit 4 qorp for coarse adjustment and 44 or w for fine adjustment Values between 1 and 1 000 can be set 5 DONE to save changes When the value set in the Number of Days Setting has been reached E4 XLS will present the following An on screen Service Alarm notification E Service Alarm Note To exit a notification message select CONT Be ee have elapsed since The Service Alarm icon will display in the Service Mode Identification Page Pass TNIE These notifications will continue to display until the Days Alarm is turned off the Number of Days Setting is re set or service is performed 1 10 E4 XLS Operational Modes General information on editing settings and operation are described in Basic Operation Using Basi
39. e operator including name sound level backlight level etc SERIAL NUMBER factory designated unique number for each pipette SERVICE LOG Historical log of last 32 services performed on the pipette Entered by technician Not user changeable SOUND LEVEL setting to turn ON or OFF the speaker i e beep during operation when ON the volume level can be set between 1 soft and 10 loud SPEED setting to decrease or increase the rate of flow in which sample is aspirated dispensed or mixed USB universal serial bus communications link versions include micro mini connectors TIME current time set in hours minutes seconds displayed on operating screen user adjustable TIME DISPLAY available formats for displaying the time user selected NONE no time display 12 HOUR 12 hour clock no AM or PM 24 HOUR 24 hour clock VOLUME setting setting for desired amount of liquid Power Management Terms BACKLIGHT SETTING a user setting for the amount of power driving the display backlight during normal use adjustable for either ease of viewing or power conservation BACK UP BATTERY coin cell internal battery used to maintain vital operating parameters e real time clock BATTERY ICON displays the state of the battery amount of charge remaining until hibernation CHARGE INDICATOR display of charging taking place flashing battery icon CHARGE CYCLE time requirements for 1 Charge Stand 2 Micro
40. e so that the tip ends are above the centers of the wells or test tube block into which sample will be dispensed Look at the scale on the liquid end manifold and note where the marked nozzle aligns Set this value on the LIMITER knob lf fhe spacing is over extended simply click the LIMITER knob to smaller values stopping when the tip ends are aligned over the centers of the wells test tubes Once the LIMITER knob has been set spacing the nozzles properly is simple 1 Aspirate the sample 2 set the nozzle spacing by moving the SPACING ADJUSTMENT knob until if stops at the value set by the LIM ITER knob 3 Dispense into the wells or test tube block Tip Immersion Depth The recommended depth for tip insertion is shown in the table below E4 XLS Model Immersion Depth EA8 50XLS 2 3 mm EA6 300XLS EA8 300XLS EA6 1200XLS 3 6 mm EA8 1200XLS Operate the pipette within 20 degrees of vertical Tip Ejection simply press on the tip ejector button All tips are ejected cleanly with minimal pressure on the thumb because of the progressive eject design built in to the liquid end manifold Positioning the Liquid End Manifold The liquid end manifold can be rotated to any angle for convenience There is no need to loosen the coupling nut Note Unique tips for 1200 uL models The RT L1200 and RT L1 2008S tips are specifically designed for use with 1200 uL models in MULTIDISPENSE mode The unique design of these ti
41. e used as guidelines when establishing your own performance specification Accuracy Precision Model Volume pL Increment pL HL HL lt 10 uL 1 0 01 2 5 0 025 1 2 0 012 5 1 5 0 075 0 6 0 030 10 1 0 100 0 4 0 040 20 uL 2 0 02 7 5 0 15 2 0 04 10 1 5 0 15 0 5 0 05 20 1 0 20 0 3 0 06 50 uL 5 0 05 3 0 0 18 1 5 0 075 25 0 8 0 30 0 4 0 100 50 0 8 0 40 0 2 0 100 100 uL 10 0 1 3 0 0 35 1 0 10 50 0 8 0 40 0 24 0 12 100 0 8 0 80 0 15 0 15 200 uL 20 0 2 2 5 0 5 1 0 20 100 0 8 0 8 0 25 0 25 200 0 8 1 6 0 15 0 30 300 uL 30 0 2 2 5 0 75 1 0 300 150 0 8 1 20 0 25 0 375 300 0 8 2 40 0 15 0 450 1000 uL 100 1 3 3 0 60 0 60 500 0 8 4 0 20 1 1000 0 8 8 0 15 1 5 1200 uL 100 2 3 6 3 6 0 60 0 6 600 0 8 4 8 0 20 1 2 1200 0 8 9 6 0 15 1 8 2000 uL 200 2 3 6 0 60 1 2 1000 0 8 8 0 20 2 2000 0 8 16 0 12 2 4 5000 uL 500 5 2 4 12 0 60 3 2500 0 6 15 0 20 5 5000 0 6 30 0 16 8 10 mL 1 mL 10 5 50 0 30 6 5 mL 1 50 0 20 10 10 mL 0 6 50 0 16 16 20 mL 2 mL 20 5 100 0 60 12 10 mL 1 100 0 20 20 20 mL 0 6 120 0 16 32 Specifications are subject to change without notice Multichannel models only in these volume ranges Electrical Specifications This device is intended for use only with Rainin power sources with the part numbers listed below No other power sources may be used with this device It is important that
42. er of aliquots by highlighting the Aliquots Setting box Aliquots or gt to edit 4 or gt for coarse adjustment and or w for fine DONE or to save CANCEL to exit without saving changes Note E4 XLS will only allow values in the Aliquots Setting that will not exceed the maximum pipette volume with the current Aliquot Volume Setting The Volume Setting will automatically adjust based on the new Ali quots Setting and the existing Aliquot Volume Setting 3 DONE to exit Settings screen and return to operating screen Volume Sequencing Option must be on for these settings fo be shown The first setting Volume Information Vol X of X displays the total aspirate volume required to dispense the full or partial set of aliquots in the series If only one aspiration is re quired to dispense the full series Vol 1 of 1 is shown If ihe series volume exceeds the pipette maximum volume it is split into multiple aspirations and Vol 1 of X is shown e g if two aspirations are required Vol 1 of 2 is shown at the initial ASPIRATE operation This will increment to Vol 2 of 2 when the next volume must be aspirated 1 To select a series of preset volumes highlight the Aliquots Setting Alq X of X or to view the volume table Use the joystick to highlight the last volume for the series Only active table cells white text can be selected END VOL to set DONE to exit table 2 To edit a preset volume use the joystick to highlight the vol
43. etie information stored in the RFID tag to be scanned and read into the software RAININ Pipette RFID Tag Read Only Fields The software will display the read only data fields programmed into each Figure 30 Rainin RFID Reader pipette s RFID tag before it leaves the factory Figure 31 These fields can ONLY be modified by Rainin at the time of manufacture or when sent in for service Read only fields include RFID UID Model Number Serial Number Factory Date Last Service Date and Next Service date For QC or workflow purposes multiple RFID enabled pipettes can be scanned sequentially Fapema Har Dir Janr F tebe T anal me Theda F mma i Dpi En qF Pipette Data hpr A Daf i har T aa Ma anaf Oats 0 sl rrual Fiat A Ud Pipette Data 3 cion cal mi pz I rd dl FF IDO LIA HOH Farior Dais eTa 2504 Made Pami y b 0001 Lae Sarar g fi panel Seal Hirig P Haj Saree Ipan pasa fal Figure 31 Read Only Pipette Data in LabX Direct Pipette Scan Software Before Scan top and After Scan bottom Writing Data to Custom Fields in the RFID Tag In addition to read only fields several writable fields are available on the RFID tag These can be customized and or standardized to suit the needs of an individual user or laboratory Custom writing operations require both the Rainin RFID Reader and the LabX Direct Pipette Scan software Please refer to the software help file for complete instructions RFID LabX Direct Pipette Scan Software Bene
44. f place the tip into the next receiving vessel y again to dispense Repeat this step for each of the remaining aliquots The series number in the Aliquot Setting will increment after each dispense lf the Auto Pace option is on DISPENSE AUTO will display Place the tip into the next receiving vessel An interval counter will display at the top of the operating screen The aliquot will dispense automatically when the set interval is reached Repeat this step for each of the remaining aliquots The series number in the Aliquot Setting will increment after each dispense 3 When aliquot dispensing is complete BLOWOUT will display ww or RESET to execute blowout ww or RESET again if more blowout s needed Volume Sequencing Operation 1 4 to select the next preset series volume in the table if needed 2 or A io aspirate 3 or w to dispense into the first receiving vessel lf the Auto Pace option is off place the tip into the next receiving vessel y again to dispense Repeat this step for each of the remaining aliquots The Aliquot Setting box will display the volume for each aliquot in the series and the series volume number will increment after each dispense If the Auto Pace option is on DISPENSE AUTO will display Place the tip into the next receiving vessel An interval counter will be shown at the top of the operating screen The aliquot will dispense automatically when the interval is reached Repeat this step for each of the
45. fits The flexibility of the LabX Direct Pipette Scan software assists users in customizing individual workflows Entire departments can standardize both workflow and calibration checks when using the E4 XLS pipettes Rainin RFID Reader and LabX Direct Pipette Scan software as a complete system The system simplifies the Quality Compliance process by shortening the calibration check cycle allowing pi pettes to be returned to active lab use faster Custom fields can be configured and written to the RFID tag and advanced workflows can be defined for cus tom settings Informationwithin the tag can be exported to common formats such as Excel and Word as well as Text to facilitate records keeping What RFID Does Not Do RFID does not protect against misplacement theft or provide a pipette s location RFID does not protect against erroneous data input RFID does not replace calibration stickers for businesses or displace QC professionals RFID does not validate laboratory workflows The validation of the workflow and 21CFR Part 11 regulatory compliance remains the responsibility of the customer 53 54 3 E4 XLS Multichannel Pipettes 3 1 Description and Operation Introduction E4 XLS multichannel pipettes are based on the E4 XLS single channel pi pette and use the patented LTS LiteTouch Tip Ejection System Multichannel EA XLS is available in 8 channel and 12 channel versions An 8 channel version is show
46. he pipette operation to ASPIRATE MAIN displays the menu the mode was accessed from HELP provides general information for the highlighted setting Operating instructions can be found in the Help for the operating screen Options and Settings Options None Settings Single Volume Single Speed Blowout Aufomatic and Manual Setup Select Mode and Settings 1 In the Main Menu q or gt to highlight BASIC Figure 8 left 2 Q A or w to enter Basic Mode Figure 8 right BASIC PREV 13 HELP MAIN z HELF Figure 8 Basic Mode Highlighted in Level Menu left Basic Mode Operating Screen righ 3 gt to enter the Settings screen The first setting box in the screen Volume will be highlighted This is indicated by a white border around the settings box Figure 9 left 4 or w to navigate between and highlight other settings 4 or to edit the volume setting The settings box will turn white to indicate it is in edit mode Figure 9 right 4 or gt for coarse volume adjustment and 44 or w for fine volume adjustment Hold the joystick in place to quickly scroll through values 2 52 74 al 2 52 25 O 200 0uL 200 0uL f f Speed DONE sh HELP DONE 48 gt CANCEL Figure 9 Basic Mode Volume Highlighted left Volume in Edit Mode right 5 DONE or to save the volume setting CANCEL to exit without saving changes The next setting speed will be highlighted Figure 10 left 6 or b
47. in Manual Mode is variable Options If pipetting options are available in an operational mode OPTIONS will be shown in the operating screen Options can be selected as follows 1 OPTIONS to access the Options screen Figure 15 left 2 A or w to navigate between and highlight an option When more than one page of options is available Options 1 of X is displayed in the upper left corner of the screen To navigate between pages 4 or w ot the first or last option on a page Figure 15 center and right 3 0 dor to turn an option on or off 4 DONE to save changes and return to the operating screen When an option is on an associated option icon will be displayed in the lower right corner of operating screen and or an additional settings box will be shown If the operational mode is exited all options are retained when the mode is accessed again ADVANCED ADVANCED Cal ADVANCED a Fixed Cycle Y olume E On Counter M On Volume Sequencing add Mix On Blowout Off MAIN 12 gt OPTIONS DONE s gt ae HELP Figure 15 Options in Advanced Mode OPTIONS Soft Key in Operating Screen left Options 1 of 2 center and Options 2 of 2 right Volume Options Fixed Volume Provides the option of selecting up to 14 preset volumes from a table in the Volume Setting box When this option is turned on the Fixed Volume Setting box will display in the operating screen This option is useful for routine work where standard
48. ing of aliquots at user settable time intervals between 0 1 and 30 sec onds Available in Multi dispense Mode only When this option is on the Auto Pace icon is displayed in the operating screen Settings The pipette settings available in each operational mode are displayed in the boxes along the right side of the operating screen Volume and speed settings are common to all operating modes Cycle count and mix settings are displayed if the corresponding option is available and has been turned on in the operational mode In general settings can be selected and changed as follows 1 P to access the Settings screen 2 or w io navigate between and highlight a setting 3 orp to edit 4 lt 4 or gt for coarse value adjustment and 44 or w for fine value adjustment Hold the joystick in place to quickly scroll through values some option specific settings may require additional steps If the operational mode is exited all settings are re tained when the mode is accessed again Volume Settings Allow users to set the volume of sample to be aspirated Minimum and maximum volumes are dependent on the EA XLS pipette model The type of Volume Setting displayed depends on what options are turned on or off in the operational mode Single Volume Allows one volume to be set and used for aspirate dispense When this setting is active Volume is displayed in the Volume Setting box This is the default volume setting for all opera
49. inue lf Manual is selected continue to hold w after dispense to execute mix cycle s Mixing will continue until the joystick is released Mixing is followed by an automatic blowout if enabled 5 w or RESET for manual blowout s if needed Titrate Mode Titrate Mode performs titration through measured dispensing Users can set an initial rapid dis pense and then precisely control dispense of the remaining titration volume The default menu A level for Titrate Mode is Level Il To access Titrate Mode 4 or gt in the Level II Menu to highlight lA A TITRATE and O A or w io enter Options and Settings Options Cycle Counter settings Single Volume with Fast Dispense Multi Speed Cycle Count Blowout Manual Options Selection 1 OPTIONS to enter the Options screen 2 or gt to turn option on or off 1 DONE or qto save and return to the operating screen Editing Settings When editing settings hold the joystick in place to quickly scroll through values 1 to enter the Settings screen 2 or w io navigate between and highlight settings Single Volume Asp Vol is displayed in Volume Setting box This value represents the maximum aspiration volume required for the titration 1 O or to edit 39 40 2 4 or for coarse adjustment and 44 or w for fine 3 DONE or to save CANCEL to exit without saving changes 4 DONE to exit Settings screen and return to operating screen Fast Dispe
50. ipetting Multidispensing Display brightness and display timeout settings 45 46 1 15 Care and Maintenance E4 XLS pipettes are sophisticated lab instruments and should be treated with appropriate care E4 XLS should provide years of trouble free service if the operating recommendations in this manual are followed The most important factor in taking proper care of E4 XLS is to keep the mechanism dry and clean The following simple rules should be strictly observed 1 Never allow liquid to enter the shaft where it can contact the piston or seal 1 Never allow liquid to enter the electronics including the display soft keys and joystick 2 Never pick up liquid without a tip attached 3 Never invert the pipette or lay it on its side with liquid in the tip Always hold E4 XLS upright and store upright if possible The Rapid Charge Stand holds three E4 XLS pipettes and charges them simultaneously 4 Never use aggressive solvents to clean E4 XLS It is best use a lint free wipe dampened with water with dilute mild detergent if needed to clean the instrument Be sure to keep the display soft keys and joystick dry 5 Never attempt to recharge E4 XLS with a device other than the Rainin E4 XLS WPS Wall Power Supply the E4 XLS RCS Rapid Charge Stand or the E4 XLS USB cable Severe damage to the internal electronics will result 1 16 Autoclaving The shaft and tip ejector arm are the only autoclavable parts of E4 XLS Autoclave a
51. ke coarse setting value adjustments Note Left 4 and right P arrows represent these joystick controls in this manual Move the joystick control up or down to Aspirate and dispense Navigate between settings and options Make fine setting value adjustments Note Up A and down Cw arrows represent these joystick controls in this manual Getting Started Battery EA XLS is shipped without the main battery installed and with the back up coin cell battery insulated so that it will be fully charged when you need it To get started open the battery cover on the back of the pipette head and remove the pull tab shown in Figure 4 the pull tab is used to insulate the battery during shipping Figure 4 Insulating pull tab Locate the main battery and and install it as shown below in Figure 5 then you will see a screen advising you to set your local time and language the default language is set to English Setting preferences is shown in section 1 9 Setup Mode Make sure the label faces outward and the battery is in the same orientation as shown in the photo Replace the battery door Chorge battery using only RAININ approved charger Do not short circuit battery terminals with wire ha pocket change etc Do bel el not remove this la modify solder or disassemble battery Do not heat or dispose of in fire Li lon 3 7V 1110mAh 4 11 Wh E Mode in China Figure 5 Installing the main battery Charge
52. l Fast Transients EN61000 4 5 Surge Power Ports EN61000 4 6 Conducted Immunity 15MHz 8OMHz EN61000 4 11 Voltage Dips and Interrupts Herewith declares that the product is in conformity with the provisions of the following EC directives Cincl all applicable amendments 2006 95 EC Low voltage LVD 2004 1 08 EC Electromagnetic compatibility EMC supplementary Information Responsible Signatory Deryl Stanley Head of R amp D Date May 2011 This Declaration of Conformity applies only to products which have the CE mark attached WARNING This equipment generates and uses radio frequency energy and if not installed and used properly i e in strict accordance with the instruction manual may cause harmful interference to radio communications It has been tested and found to comply with the limits for a Class A computing device pursuant to Subpart B of Part 15 of FCC rules which are designed to provide reasonable protection against such interference when operated in a commercial environment Operation of this equipment in a residential area is likely to cause interference in which case the user at his own expense will be required to take whatever measures may be required to correct the interference 63 Rainin Instrument LLC 7500 Edgewater Drive Oakland CA 94621 Sales 1 510 564 1600 Service 1 510 564 1600 Subject to technical changes O 2011 Rainin Instrument LLC Printed in USA Part Number 9920 396 Rev A a METTLER TO
53. ly to the first stop used for dense or viscous liquids SECOND STOP hard stop at the end of blow out STEP small incremental dispense steps TIP EJECT a manual step at the end of an operation that removes the pipette s tip TITRATE mode dispensing carefully measured amounts fo a solution fo cause a reaction VOLUME SEQUENCING optional setting ability to program up to 16 different aliquot volumes in a series SEQ VOLUMES available set volumes selected from a list UL a unit of measure microliter mL unit of measure milliliter General Terms AUTO OFF setting duration after last usage before automatic power shutdown CALIBRATION precision and accuracy verification of the instrument under controlled conditions CANCEL soft button used to terminate the current sequence may go to blowout amp return to home CAROUSEL method of displaying a menu in a continuous cyclic pattern CYCLES SNC SERVICE number of cycles recorded since service DATE setting user adjustable calendar time DATE FORMAT available calendar formats in which the day month amp year are displayed M D Y month day year Y M D year month day D M Y day month year DEFAULT setting preselected values typically the most commonly used value DISPLAY BRIGHTNESS setting the level of backlighting for the LCD screen DISPLAY TIMEOUT setting the number of seconds before display dims up to 120 seconds FIRMWARE
54. n icon In the example in Fig 3 there is a function shown for the left soft key RESET but not for the right one Left soft key items that can be displayed MAIN Displays the Main Menu for the menu level selected PREV Returns fo the previously used mode allows toggling between modes DONE Exits Settings and Options screens after activity is done RESET Empties tip and resets operation RETURN Exits a Help screen and returns to the previous screen CONT Exits notification messages and allows the current operation to continue Right soft key items that can be displayed OPTIONS Available mode options CANCEL Cancels and exits the current action without saving changes HELP Displays mode and menu information and operating instructions Other mode and setting specific functions MULTI DISP 04M CI DISPENSE 190 0uL Volume 38 0uL Aliquot Vol Aliquots Dsp Speed Figure 3 E4 XLS Controls 1 3 Joystick Thumbstick Controls Rainin recommends that users practice menu and mode screen navigation in order to familiarize themselves with the joystick s ease of use and sensitivity in control Press and click the center button to Access menu items Edit settings or options Choose and save selections Note The circle icon represents a center press control in this manual Move the joystick control left or right to Navigate between menu items and pages Access Settings screens Ma
55. n in Figure 32 The 8 and 12 channel models are available in 6 volume ranges 0 5 10 uL 2 20 uL 5 50 uL 20 200 UL 20 300 uL 100 1200 uL Aj Tip Selection E4 XLS multichannel pipettes use Rainin LTS tips Use 20 uL LTS tips with the following E4 XLS models ES 10XLS ES 20XLS E8 12 10XLS and E12 20XLS Use 250 uL LTS tips with the following models E8 50XLS E12 50XLS E8 200XLS and E12 200XLS Use 300 uL LTS tips with E8 300XLS and El2 300XLS 1200 uL LTS tips are recommended for E8 1200XLS and E12 1200XLS Non filter 1000 uL LTS tips can also be used as they will pick up 1200 UL RAININ 20 300 pl LTS LTS tips have a cylindrical cross section with a well defined seal ring thin wall and positive stop They seal properly on the shaft and cannot be jammed or forced too far onto the shaft nozzles and provide absolutely con sistent sample pickup across all channels Figure 32 E4 XLS MultiChannel Pipette Mounting LTS Tips Mounting racked LTS tips on L8 and L12 pipettes is simple 1 Align the shaft nozzles into the row of tips holding the pipette at an angle Figure 33 left 2 Position the pipette upright and press the nozzles into the tips until the positive stop is reached Figure 33 right The tips are now mounted with proper sealing There is no need to hand tighten use heavy pressure or rock the nozzles onto the tips to obtain a good seal ral F Figure 33 Mounting Racked LTS Ti
56. nse Volume The Fast Dispense Setting Fast Dsp Vol represents the first initial volume of sample to dispense prior to start ing the fine dispense control for the titration Note If this value is set to 0 0 uL the fine dispense control will begin immediately after aspiration 1 orp to edit orp for coarse adjustment and 44 or w for fine DONE or to save CANCEL to exit without saving changes amp w DONE to exit Settings screen and return to operating screen Speed 1 or gt to view individual operation speeds Cycle Speeds 2 or w to highlight setting or gt to edit Aspirate 3 lt or for coarse speed adjustment of 1 5 or 10 and Am or w for fine adjustment in Dispense 10 single digits 4 DONE or to save CANCEL to exit without saving changes DONE fo exit table 5 DONE to exit Settings screen and return to operating screen Cycle Count Option must be on for this setting to be shown 1 orp to edit 2 RESET to zero counter 4 or for coarse adjustment and 44 or vw for fine 3 DONE or to save CANCEL to exit without saving changes 4 DONE to exit Settings screen and return to operating screen Operation The active operation will be displayed in the upper left of the operating screen When aspirating or performing a Fast Dispense it is not necessary fo hold the joystick in place E4 XLS will automatically complete the operation At any time during operation RESET em
57. o view mix settings 4 or w lo Eeim highlight setting Cycles 8 1 To change volume or to edit or for coarse adjustment and 44 or w for fine DONE or to save CANCEL to exit without saving changes DONE to exit table 2 To change cycles or gt to edit 4 or p for coarse adjustment and 4 or w for fine up to 99 cycles ww at 1 for Manual DONE or to save CANCEL to exit without saving changes DONE to exit table 3 DONE to exit Settings screen and return to operating screen Cycle Count Option must be on for this setting to be shown 1 orp to edit 2 RESET to zero counter 4 or gt for coarse adjustment and or w for fine 3 DONE or to save CANCEL fo exit without saving changes 4 DONE fo exit Settings screen and return to operating screen Operation The active operation will be displayed in the upper left of the operating screen When aspirating and dispensing it is not necessary to hold the joystick in place E4 XLS will automatically complete the operation At any time during operation RESET empties the tip and resets the pipette operation to ASPIRATE MAIN displays the menu the mode was accessed from HELP provides general information for the highlighted setting Operating instructions can be found in the Help for the Volume Setting Single and Fixed Volume Operation 1 Single Volume operation skip to step 2 Fixed Volume operation 4 to select the next fixed volume in the table if needed
58. onnnoncnnnnnnncnss 62 EC Declaration of Conformity 0 00 0 ieee ron n rn narran nano r rn rn narra naas Inside back Cover 1 1 1 2 E4 XLS Electronic Pipette Introduction Rainin s E4 XLS is a revolutionary electronic pipette fully laden with features and with a new graphical user in terface that is extremely straightforward and intuitive in operation The joystick control provides an easy to un derstand logic for setting operating modes and for aspi ration and dispensing a variety of liquids The pipette is designed for maximum comfort In models with the patented LTS LiteTouch Tip Ejec tion System tip ejection forces are reduced by up to 85 in the single channel LTS models and consistent sample pickup is easily attained across all channels in multichannel models as well as tip ejection force reduction E4 XLS is available in single multichannel and adjust able spacer versions All user settings and service settings are stored on non volatile memory and are protected even if the pi pette is reset or in the unlikely case of a battery failure E4 s built in Service GLP section help you track the pipette s service records and intervals E4 XLS models also contain an RFID tag radio frequency identifica tion for facilitating calibration management for all your pipetie assets E4 XLS Pipette Overview Before using E4 XLS for the first time please review the instrument components user interface and pipe
59. or coarse adjustment and 44 or w for fine DONE or to save DONE to exit table Cycles G 2 To change cycles or p to edit 4 or gt for coarse adjustment and 44 or w for fine up to 99 cycles wy at for Manual DONE or to save CANCEL to exit without saving changes DONE to exit table 3 DONE to exit Settings screen and return to operating screen Cycle Count Option must be on for this setting to be shown l orp to edit 2 RESET to zero counter 4 or gt for coarse adjustment and 44 or vw for fine 3 DONE or to save CANCEL to exit without saving changes 4 DONE to exit Settings screen and return to operating screen Operation The active operation will be displayed in the upper left of the operating screen When aspirating and dispensing it is not necessary to hold the joystick in place E4 XLS will automatically complete the operation At any time during operation RESET empties the tip and resets the pipette operation to ASPIRATE MAIN displays the menu the mode was accessed from HELP provides general information for the highlighted setting Operating instructions can be found in the Help for the Volume Setting Single Volume Operation 1 Asp 1 will display in the Volume Setting box or A fo aspirate diluent 2 Remove the pipette tip from the diluent AIR will display in the Volume Setting box or x fo aspirate an air gap 3 Place the pipette tip in the next sample Asp 2 will dis
60. ou of important issues such as battery levels USB connection charge stand connection factory reset real time clock status and more Any changes to these variables that could affect your operation of the pipette will be reported as a Warning message Some warnings require action such as recharg ing the unit or resetting the clock while others are informational only Memory Clear after factory reset To exit any warning message press the left softkey CONT 4 48 lf a warning message is displayed during the E4 XLS power up sequence that cannot be reset note the error number and contact Tech Support at 800 543 4030 in the US In other countries contact METTLER TOLEDO or your distributor Error Messages E4 XLS is a sophisticated product that is dependent on a complex mix of electronic hardware and software components In any complex system such as this faults may occasionally occur These are usually due to un expected software conditions invalid data conflicting settings etc or hardware related failures invalid or no response An Error message informs you that a fault has been detected Press the left softkey CONT and the unit will reset itself while performing a diagnostic test Most of the time the unit will fix itself and pipetting can be resumed normally If the error messages continue please cease pipetting note the error code and message and call Tech Support at 800 543 4030 in the US In other countries contact ME
61. pense and mix speed settings to be set individually Displays ae aspirate and dispense speeds Asp Dsp Spd initially During operation the set value and cur rent operation Asp Speed Dsp Speed or Mix Speed will be shown in the settings box as each action is carried out Cycle Count A Cycle is complete aspiration and dispense followed by blowout Displays the operational mode s current cycle count which increments after each cycle completes Cycle count can be set to any value between O and 99 999 This setting is shown only when the Cycle Counter option is on Mix Allows users to perform in tip mixing after dispense A mix volume up to 99 automatic mix cycles or manual mixing can be selected This setting is shown only when the Mix option is on Minimum and maximum volumes are dependent on the size of the E4 XLS pipette When auto EM matic mix cycles are used the set volume and number of mix cycles Mix Cyc X are shown in the settings box The cycle number will increment as each mix cycle completes until the set number of cycles is reached When manual mixing is used the set volume and Mix Manual are shown in the settings box Using the Pipette Pipetting Guidelines E4 XLS pipettes incorporate several features to enhance pipetting consistency In addition these guidelines should also be followed e Use a consistent immersion depth e Pipette vertically or within 20 degrees of vertical e Pre rinse the tip twice by aspi
62. perating screen Speed 1 or gt to view individual operation speeds Cycle Speeds 2 or w io highlight setting or gt to edit Aspirate 3 orp for coarse speed adjustment of 1 5 or 10 and A or w for fine adjustment Dispense 10 in single digits 4 DONE or to save CANCEL to exit without saving changes DONE fo exit fable 5 DONE fo exit Settings screen and return to operating screen Cycle Count Option must be on for this setting to be shown 1 or to edit 2 RESET to zero counter 4 or for coarse adjustment and A or vw for fine 3 DONE or to save CANCEL to exit without saving changes 4 DONE to exit Settings screen and return to operating screen Operation The active operation will be displayed in the upper left of the operating screen When aspirating and dispensing it is not necessary to hold the joystick in place E4 XLS will automatically complete the operation At any time during operation RESET empties the tip and resets the pipette operation to ASPIRATE 35 36 MAIN displays the menu the mode was accessed from HELP provides general information for the highlighted setting Operating instructions can be found in the Help for the Volume Setting Single and Fixed Volume Operation 1 Single Volume operation skip to step 2 Fixed Volume operation lt 4 to select the next fixed volume in the table if needed 2 or A to aspirate selected volume plus blowout volume
63. play in the Volume Setting box or A to aspirate 4 or w to dispense Each dispense is followed by an automatic blowout if enabled If the Mix option is on When a set number of cycles is selected mixing will execute automatically after each dispense followed by an automatic blowout if enabled Select PAUSE at any time to pause mixing then RESUME to continue If Manual is selected continue to hold w after dispense to execute mix cycle s Mixing will continue until the joystick is released Mixing is followed by an automatic blowout if enabled 5 w or RESET for manual blowout s if needed Volume Sequencing Operation 1 Vol 1 of X will display in the Volume Setting box 4 to select the next preset series volume in the table if needed Place the pipette tip in the first sample or A to aspirate 2 Vol 2 of X will display in the Volume Setting box Place the pipette tip in the next sample or diluent or re move the tip completely from liquid for an air gap or A to aspirate 3 Repeat Step 2 until all samples in the series are aspirated The series number in the Volume Setting box will increment after each aspiration 4 or w to dispense Each dispense is followed by an automatic blowout if enabled If the Mix option is on When a set number of cycles is selected mixing will execute automatically after each dispense followed by an automatic blowout if enabled Select PAUSE at any time to pause mixing then RESUME to cont
64. ps Tip Immersion Depth The recommended depth for tip insertion is shown in the table below E4 XLS Model Immersion Depth E8 10XLS E12 10XLS E8 20XLS E12 20XLS 2 3 mm E8 200XLS E12 200XLS E8 300XLS E12 300XLS 3 6 mm E8 1200XLS E12 1200XLS Operate the pipette within 20 degrees of vertical Tip Ejection Simply press on the tip ejector button All tips are ejected cleanly with minimal pressure on the thumb because of the progressive eject design built in to the liquid end manifold Positioning the Liquid End Manifold The liquid end manifold can be rotated to any angle for convenience when filling plates There is no need to loosen the coupling nut Note Unique tips for 1200 uL models The RT L1200 and RT L1200S tips are specifically designed for use with 1200 uL models in MULTIDISPENSE mode The unique design of these tips prevents drop formation and eliminates the need for touch off between dispenses in MULTIDISPENSE mode The RT L1200F aerosol resistant tip allows a full 1200 uL to be aspirated under the filter However fluid dynamics dictate that touch off may be required when multidispensing 99 56 4 E4 XLS Adjustable Spacer Pipette 4 1 Description and Operation Introduction E4 XLS Adjustable Spacer multichannel pipettes are based on E4 XLS single channel models and operate in the same way Two versions of the E4 XLS Adjustable Spacer pipette are available 6 channel LA6 and 8 channel L
65. ps prevents drop formation and eliminates the need for touch off between dispenses in MULTIDISPENSE mode The RT L1200F aerosol resistant tip allows a full 1200 uL to be aspirated under the filter However fluid dynamics dictate that touch off may be required when multidispensing 9 Appendices 5 1 Speed Table single Channel 10 20 100 200 300 1000 2000 uL 8 12 Channel 10 20 50 200 300 uL Single Channel 5 10 20 ML 8 amp 12 Channel 1200uL 2 O Om 13 eT 29145 1465 HHO 8 190 190 06 o 10 7 239 239 08 05 10 Zo a 08s 0 to o5 346 348 1 og O So a oS 08 0 3 4w 490 2 5 580 19 142 10 Ol 742 IA 25 178 10 60 5 2 Glossary of Terms used in E4 XLS and this Manual Pipetting Terms ADVANCED Advanced Pipetting mode AIR air gap between two aspirations within the same pipetting cycle ALIQUOTS a sample dispensed from a larger volume typically refers to an individual sample of multi dispense ALIQUOT VOL a list of available preset volumes ASPIRATE to pick up liquid into the pipette tip ASP SPEED aspirate speed ASP DISP aspirate and dispense speeds AUTO PACE a programmable timed event for each multi dispense aliquot BLOWOUT fo run piston to the end of stroke empty the tip of liquid CYCLE COUNTER abr CC a setting which counts the total number of pipetting cycles CYCLE SPEEDS aspirate dispense and
66. pties the tip and resets the pipette operation to ASPIRATE MAIN displays the menu the mode was accessed from HELP provides general information for the highlighted setting Operating instructions can be found in the Help for the Volume Setting 1 or A io aspirate 1 12 2 Ifa value was set in the Fast Dispense Setting FAST DISPENSE will display or w to dispense If this value was set fo zero skip to the next step 3 DISPENSE will display and the Fast Dispense Setting box will now show Dsp Total w slowly to begin titrat ing the remaining volume As the joystick is moved towards its maximum downward position larger volumes will be titrated at a faster rate To titrate smaller volumes at slower rates move the joystick slowly until the desired rate is achieved lf the joystick is released operation will pause When joystick control resumes operation will continue from the same point STEP will dispense the pipette s minimum allowable increment each time the soft key is pressed During the titration the Dsp Total value will automatically update with the total volume dispensed includ ing the Fast Dispense volume 4 When the titration is complete the value in the Dsp Total represents the total titration volume The volume displayed in the lower left of the operating screen is the volume of sample that remains in the tip 5 ww and hold or RESET fo release the remaining sample from the tip 6 w or RESET for manu
67. rating and dispensing sample before actual pickup e Do not invert or lay the pipette flat with liquid in the tip More information on Good Pipetting Technique including a Lab Poster can be found on the MT website www mt com rainin Tip Selection and Mounting Rainin pipettes and tips are designed together as a pipetting system E4 XLS pipettes are calibrated with Rainin tips Performance to published specifications can only be guaranteed when Rainin tips are used To mount a tip press the E4 XLS shaft into the end of the tip with light force With both LTS and traditional ver sions the tip will seal properly on the shaft with minimal force do not use more force than is required 1 6 3 Tip Immersion Depth The recommended depth for tip insertion into the sample for each model is shown below Nominal Volume Volume Range Immersion Depth 10 uL 0 5 to 10 uL 1 2 mm 20 uL 2 to 20 uL 2 3 mm 100 uL 10 fo 100 uL 2 3 mm 200 uL 20 to 200 uL 3 6 mm 300 uL 20 to 300 UL 3 6 mm 1000 uL 100 to 1000 uL 3 6 mm 2000 uL 200 to 2000 uL 3 6 mm 5000 uL 500 to 5000 uL 6 10 mm 10 mL 1 to 10 mL 6 10 mm 20 mL 2 to 20 mL 6 10 mm Tip immersion depth is critical If the recommended depths are exceeded the volume measured may be inac curate and possibly out of specification The tip angle is also important The pipette should always be used in a position within 20 degrees of vertical as shown in Figure 16
68. ration into the tip and its red guide arrow and active volume display will adjust accordingly 4 Pause for approximately one second longer for large volume pipettes to ensure that the full volume of sample is drawn into the tip 5 Withdraw the tip from the sample If any liquid remains on the outside of the tip touch it off carefully taking care not to touch the tip orifice Dispense The mode operation will now flash DISPENSE indicating E4 XLS is ready to dispense sample The joystick con trols used for dispense will also flash in the navigation icon Figure 12 123 4pL RESET Figure 12 Basic Mode Dispense Operation 1 Touch the tip end against the side wall of the receiving vessel or w to dispense the sample It is not nec essary to hold the joystick in place E4 XLS will automatically complete the operation The Tip Volume Indica tor will mimic sample dispense from the tip and its red guide arrow and active volume display will adjust accordingly Wait 1 second for 2 300 uL volumes and 1 2 seconds for 1000 uL volumes or higher 2 Withdraw the tip sliding it along the wall of the vessel 3 l needed wy or RESET for tip blowout w or RESET again as needed for more blowout s Figure 13 For viscous solutions pause before blowout Note that Blowout is automatic but you can also perform blowout manually fo repeat You can delay piston return time if desired by holding down the joystick Figure 13 Basic Mod
69. remaining aliquots The Aliquot Setting box will display the volume for each aliquot in the series and the series volume number will increment after each dispense 4 When aliquot dispensing is complete BLOWOUT will display ww or RESET to execute blowout w or RESET again if more blowout needed 5 If more than one aspirate volume is required to dispense the full series the series number in the Volume In formation Setting will increment and ASPIRATE will display 6 Repeat the steps above until the series is complete Manual Mode Manual Mode provides joystick control of E4 XLS that simulates manual pipette operation Additionally fine control of precise volume increments allows aspiration and dispense of mea sured sample volumes The default menu level for Manual Mode is Level To access Manual AECE Mode 4 or gt in the Main Menu to highlight MANUAL and A or w to enter Options and Settings Options Fixed Volume Cycle Counter Settings Single and Fixed Volume Single Speed Cycle Count Blowout Manual Options Selection 1 OPTIONS to enter the Options screen 2 or w io navigate between and highlight options 3 or gt to turn option on or off 4 DONE or 4 to save and return to the operating screen Editing Settings When editing settings hold the joystick in place to quickly scroll through values 1 p to enter the Settings screen 2 4M0r w to navigate between and highlight settings Single
70. res a continuous source of power If the main Li lon battery fails or is removed and no external power source is applied such as wall power or rapid charge stand the clock runs off a back up coin cell battery This battery will keep the clock running for about 90 120 days If ihe coin cell battery drains completely a notice will remind you to reset the time and date after power is restored to the unit A new coin cell battery will be needed to provide back up power for the clock if desired For more information please contact Technical Support at 800 543 4030 in the US or your METTLER TOLEDO office or distributor outside the US EC Declaration of Conformity according to ISO 17050 Manufacturer s Name Rainin Instrument LLC Manufacturer s Address 7500 Edgewater Drive Oakland CA 94621 USA declares that the following product Product Name E4 XLS Electronic Pipette Model Number E4 XLS Product Options E4 WPS Wall Power Supply E4 RCS Rapid Charge Stand E4 RCSWPS Wall Power Supply for Rapid Charge Stand conforms to the following Product Specifications Safety IEC EN 61010 1 2001 Emissions Testing EN 61326 1 2006 Testing performed to Class A Limits commercial industrial environment Radiated Emissions SOMHz 1GHz Conducted Emissions Power ports Immunity Testing EN 61326 1 2006 EN61000 4 2 Electrostatic Discharge 4kV Air amp 4kV Contact EN61000 4 3 Radiated Immunity 8OMHz 1000MHz 1400MHz 2700MHz EN61000 4 4 Electrica
71. sses along support leg The E4 XLS RCS Rapid Charge Stand will charge three E4 XLS pipettes simultaneously in about 90 minutes Charging contacts mate with contacts under the pipette head Rainin recommends storing E4 XLS on the stand when not in use This practice will provide a safe storage place and E4 XLS will always be fully charged USB Cable To charge E4 XLS with a PC using the optional USB cable connect the cable s USB connector to a computer USB port Connect the other end of the cable to the USB outlet located on the top of E4 XLS Rainin does not recom mend operating E4 XLS when the pipette is connected to a computer for charging Note that charging E4 XLS with a PC will take approximately 2 3 times longer than using the Wall Power Sup ply or the Rapid Charge Stand 1 14 Replacing the Battery E4 XLS user settings are retained if the battery is removed or replaced 1 Open the battery compartment by sliding the compartment door open 2 Remove the old battery by sliding if out 3 Align the replacement battery connector plug with the socket in the battery compartment as shown in Figure 28 Slide the battery into place Once the battery is installed properly E4 XLS will turn on automatically ini tialize and display the start up screen Figure 28 Replacing the Battery Replace the battery compartment door The long battery life is dependent on such factors as Pipette volume range Full range or part range p
72. t 121 C at 1 bar for 15 20 minutes DO NOT autoclave the complete pipette or any parts other than the shaft and the tip ejector arm 1 17 Tip Ejector Arm Removal Three types of tip ejector arms are used with E4 XLS pipettes All can be removed with minimal effort do not use force Pipettes up to 2000 pL Press in the quick release tabs on the ejector arm and pull the ejector down 5000 uL and 10 mL pipettes Grasp the top of the ejector arm and pull out and then down 20 mL pipette Pull off the lower part of the ejector arm the upper part stays in place Refer fo Figure 29 for a visual guide on removing the tip ejector arm on all models lt 2000 uL 5000 uL 10 mL 20 mL yo y 4 y Figure 29 Removing the Tip Ejector Arm Replacing the tip ejector arm all models except 20 mL Insert the shaft through the large opening Align the top with the tip ejector push rod and push until the ejector arm snaps in place Replacing the tip ejector arm 20 mL model Align the rod with the hole in the upper part and press firmly 1 18 Storage After use store the pipette in a clean safe place E4 XLS is a precision instrument and should be treated with the level of care appropriate for laboratory instrumentation Rainin recommends storing E4 XLS on the Rapid Charge Stand if one is available If not three hangers are avail able to hold E4 XLS conveniently when not in use CR 7 Free standing carousel that holds three pipettes
73. talled Status Page This page displays the pipette s usage logs Figure 24 These values are tracked and logged automatically and are not user settable Days Since Service Logs the number of days the pipette has been in use since it was last serviced Cycles Since Service Logs the number of aspirate dispense cycles the pipette has executed since it was last serviced Lifetime Cycles Logs the total number of aspirate dispense cycles the pipette has executed since it was first put into active use Note The Days and Cycles Since Service logs will reset to zero after each pipette service is performed SERVICE 5 44PM Lal Days since Service Cycles Sne Service Lifetime Cycles MAIN 42 gt HELP Figure 24 Status Page Service Log This page displays a historical log of the last 32 services performed on the pipette Figure 25 This information is entered by a service technician and is not user editable SERVICE 13 44 19 CE 01 gt 05 04 2011 15 21 TB CC 36 96 MAIN 42 gt HELP Figure 25 Service Log For each service entry logged The first line in an entry contains the date and time of service and technician ID The next line in an the entry contains the number of cycles executed since the last service and the total num ber of lifetime cycles at the time service was performed 1 13 Battery Charging Unlike other battery types the Li lon battery in E4 XLS has no memory effect and does not
74. the battery for at least 15 minutes before using the pipette for the first time Pipettes can be charged us ing the Wall Power Supply included with the pipette the optional Rapid Charge Stand or with a computer using the optional USB cable If the Wall Power Supply is used E4 XLS can be operated while it is charging as shown in Figure 6 Rainin does not recommend operating the E4 XLS when the pipette is connected to a computer for charging Please refer to Battery Charging on page 44 for more information on charging the battery as well as installing and using the Wall Power Supply Rapid Charge Stand and the optional USB cable Figure 6 Using E4XLS while connected by the power cord LABA O METTLER TOLEDO Power Up Press one of the soft keys to turn E4 XLS on The pipette will initial E4 10xXL5 ize and show the start up screen momentarily before displaying the Level Menu See Figure 7 E A Note that your start up screen may have different text than this image Version 1 0 44 580 LT a Figure 7 Start up Screen Basic Operation Using Basic Mode Rainin recommends learning basic pipetting operations as well as the selection and editing of settings in Basic Mode first before moving on to advanced operational modes Rainin also recommends that users practice aspi ration and dispense with water first prior to working with valuable samples At any time during operation RESET empties the tip and resets t
75. the format of the on screen time display Select 24 or 12 hour formats or None to turn the time display off 1 orp to edit 2 or w io select display format 3 DONE or to save The format of the time display in all E4 XLS screens will update immediately Date Format This is the year month and day format for the Date Setting M D Y Y M D and D M Y formats can be selected 1 orp to edit 2 A or w io select format 3 DONE or to save The Date Setting will update automatically User Preference Settings Available user preference settings are shown in Figure 20 SETUP 2 33PM ii Language English Owner Mode Levels Change MAIN 42 gt HELP Figure 20 Setup Mode Page 3 of 4 Language Users can select one of the following languages English Japanese Chinese French Spanish or German 1 orp to edit 2 or w io select language 3 DONE or to save The text displayed in all E4 XLS screens will update to the new language immediately Owner This setting identifies the pipette owner Users can enter up to 16 characters The owner will also display in the splash screen 1 orp to edit 2 The first character will be selected initially 4 or p to navigate between the other characters 3 allows selection of characters in the following order CAPITAL letters numbers lower case letters and special characters wy to reverse the selection order at any time and w at A to insert a space 4
76. tional modes and the only volume setting available in Basic Mode Fixed Volume Allows one of 14 preset volumes to be selected from a volume table This setting is shown only when the Fixed Volume option is on When this setting is active Fixed Vol X is displayed in the volume setting box For example Fixed Vol 3 indicates the volume shown is the third volume selection in the table Volume Sequencing Allows up fo 16 preset volumes to be selected for use in series from a volume table This setting is shown only when the Volume Sequencing option is on When this setting is active Vol X of X is displayed in the volume setting box For example Vol 2 of 4 in dicates the volume shown is the second of four volumes in the series selected in the table The volume value and number will increment as each cycle completes Speed Settings Allow users to set aspirate dispense and mix speeds from 1 Glow to 10 fast High speed settings are best for aqueous samples and lower speed settings are best for viscous foaming delicate or shear sensitive samples The maximum speed settings for large volume E4 XLS Basic Models are limited to prevent fountaining or air intake during aspiration See Appendix A for a complete speed table SA Single Speed Allows one speed value to be set which is used for both aspirate and dispense This is the only speed setting available in Basic Mode 1 6 1 6 1 1 6 2 Multi Speed Allows aspirate dis
77. tte controls as described on the next few pages Aa UN P 2 mM NS O O N O USB Charger Port Display Soft Keys Finger Hook RFID Tag Serial No Joystick also called Thumbstick Tip Ejector Button Shaft Tip Ejector Arm Disposable Tip Figure 1 E4 XLS Single channel Pipette Display The high performance electronic color display is the user interface for E4 XLS Screen navigation pipette opera tion settings and options selections are performed using the soft keys and joystick controls Common screen information and the available functionality in menu and mode screens are shown in Figure 3 Current menu or mode 1 3 Menu level current operation or page number Time Battery charge indicator 6 Carousel of modes Menu Level and Level ll access icon E hd Soft key functions 7 8 Basic Mode right oo SS Pl e w 0 Tip Volume Indicator Navigation Joystick navigation icon Figure 2 Display Components Level Menu left Navigation on E4 XLS is done using the soft keys and the joystick control The functions of the soft keys and joy stick control change according to the menu or mode displayed Soft Key Functions There are two soft keys below the display Left and right soft key functions change according to the current menu or mode screen Functions are shown in the display above each soft key and the available joystick controls are highlighted in the navigatio
78. ume or gt to edit Both active and inactive cells can be edited 4 or gt for coarse adjustment and or w for fine DONE or to save Repeat as needed for other volumes CANCEL to exit without saving changes DONE to exit table Note If an END VOL is not set before exiting the table the END VOL last selected will be retained 3 DONE to exit Settings screen and return to operating screen Speed 1 orp to view individual operation speeds Cycle Speeds 2 or w to highlight setting or gt to edit peprae Dispense 10 Mix 5 3 dor gt for coarse speed adjustment of 1 5 or 10 and or w for fine adjustment in single digits 4 DONE or O to save CANCEL to exit without saving changes DONE to exit table 5 DONE to exit Settings screen and return to operating screen Operation The active operation will be displayed in the upper left of the operating screen When aspirating and dispensing it is not necessary to hold the joystick in place E4 XLS will automatically complete the operation At any time during operation RESET empties the tip and resets the pipette operation to ASPIRATE MAIN displays the menu the mode was accessed from HELP provides general information for the highlighted setting Operating instructions can be found in the Help for the Volume Setting Single Volume Operation 1 or A to aspirate 2 or w io dispense into the first receiving vessel lf the Auto Pace option is of
79. verse Mode is Level Il To access Reverse Mode q or gt in the Level II 33 33 Menu to highlight REVERSE and A or w io enter Options and Settings Options Fixed Volume Volume Sequencing Mix Blowout Cycle Counter settings Single and Fixed Volume Volume Sequencing Multi Speed Cycle Count Blowout Automatic and manual Options Selection l 2 3 4 OPTIONS to enter the Options screen amp or w io navigate between and highlight options or to turn option on or off DONE or 4 to save and return to the operating screen Editing Settings When editing settings hold the joystick in place to quickly scroll through values 1 2 _ to enter the Settings screen or w io navigate between and highlight settings Single Volume Volume is displayed in Volume Setting box gt Y N orp to edit orp for coarse adjustment and 44 or w for fine DONE or to save CANCEL to exit without saving changes DONE to exit Settings screen and return to operating screen Fixed Volume Fixed Vol X is displayed in Volume Setting box Option must be on for this setting to be shown or b to view the volume table The volume currently selected will be high lighted in the table 1 2 Fixed Volumes ul To select a preset volume use the joystick to highlight a volume Only active table cells white text can be selected DONE to set and exit table To add or remove active cells in the t
80. vides multi aliquot dispensing with user settable automatic dis pense intervals The default menu level for Multi Dispense Mode is Level To access Multi Dispense Mode 4 or gt in the Main Menu fo highlight MULTI DISP and A or w to MULTI DISP enter Options and Settings Options Volume Sequencing Auto Pace Settings Single Volume with Number of Aliquots and Aliquot Volume Volume Sequencing Multi Speed Blowout Manual Options Selection 1 OPTIONS to enter the Options screen 2 or w io navigate between and highlight options Volume Sequencing O or to turn option on or off 1 DONE or 4 to save and return to the operating screen Auto Pace 1 orp to edit 2 Intervals from 0 1 to 30 seconds can be set 4 or gt for coarse adjustment and 44 or w for fine 4 at 2 0 or w at 0 1 for Off 3 DONE or to save CANCEL fo exit without saving changes 4 DONE to return to the operating screen Editing Settings When editing settings hold the joystick in place to quickly scroll through values 1 gt to enter the Settings screen 2 or w io navigate between and highlight settings Single Volume Dispensing Based on Number of Aliquots 1 Highlight the Aliquots Setting box Aliquots or gt to edit 4 or for coarse adjustment and A or w for fine DONE or to save CANCEL to exit without saving changes Note E4 XLS will only allow values in the Aliquots Setting that will not exceed the m
81. volumes are used repetitively Note repeat pressing 4 will toggle through the values Volume Sequencing Provides the option of selecting up to 16 preset volumes in series from a table in the Volume Setting box This option is useful for preparative protocols that require multiple sam ples at various volumes When this option is turned on the Volume Sequencing icon and Setting box will display in the operating screen Note Only one volume option can be on at a time For example if Fixed Volume is turned on when Volume Se quencing is already on E4 XLS will automatically turn Volume Sequencing off If both options are off the mode defaults to Single Volume Mix Option setting box will display in the operating screen Users can specify mix volume and either manual oO Provides in tip mixing of dispensed samples When this option is turned on the Mix icon and Mix mixing or up to 99 automatic mix cycles Blowout Option When this option is off the Blowout Off icon will display in the operating screen Users can still per ES Allows automatic blowout to be turned off in operational modes where blowout is on by default form manual blowout s when the Blowout option is off Cycle Counter Option a Counts each pipetting cycle which consists of one aspirate and one dispense and blowout op eration When this option is on a Cycle Count Setting box is displayed in the operating screen Auto Pace Option Provides automatic dispens
82. y to release sample or air from the tip or STEP to release a minimum increment 5 Repeat the steps above as necessary until the liquid in the tip is level with the tip orifice The in tip sample volume is displayed in the lower left of the operating screen and represents the measured volume 6 ww and hold or RESET to release sample from the tip 7 y or RESET for manual blowouts if needed Level Il Menu The Level ll Menu provides access to more advanced modes as well as Ser vice mode where users can view service information and set service alarms The default Level Il operational mode selections include Reverse Mode Dilute Mode and Titrate Mode To access the Level II Menu 4 or gt in the Level Menu to highlight LEVEL 11 a and A or w to enter TO LEVEL Level II Menu Navigation dor to view and select menu items PREY 42 SETUP Figure 22 Level Il Menu A or w io enter the selected menu item Select LEVEL 1 to return to the Level Menu PREV to save changes and return fo the previously viewed screen HELP for a detailed description of the highlighted menu item SETUP when Level is highlighted to go directly to Setup Mode and change pipette settings 33 34 Reverse Mode Reverse Mode is an established pipetting technique that enables users to aspirate the selected volume along with the pipette blowout volume and is recommended for dense or volatile liquids The default menu level for Re

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