`11` Syringe Pump Series User`s Manual
Contents
1. intensity can be set on the vacuum fluorescent display To set the desired display intensity at the RATE VOL CONFIG display move the underline cursor using the A or V key to CONFIG Press the SET key to enter the CONFIG mode options Use the N or V key to scroll the CON FIG options until you reach the SET INTENSITY Press the SET key again moving the underline cursor to the right Use the A or V key to scroll the SET INTENSITY choices 1 2 3 or 4 4 is highest intensity 1 is low est intensity Press the ENTER key to select the desired display intensity Exit the CONFIG mode by pressing the ENTER key again and save the INTEN SITY setting in memory Changing Rates If the pump is running at an existing rate it will continue to do so until a new rate is entered Except for volume mode the flow rate can be changed while the pump is running As soon as the ENTER key is pressed the pump will change to the new flow rate To change rates from the keypad while in vol ume mode the pump must be stopped first Maintenance No special maintenance is required other than keeping the pump 11 clean and dry Avoid liquid spills that may find their way into the electronics A small container of grease is provided for periodic lubrication of the lead screw A small bottle of oil is provided for periodic lubrication of the two steel guide rods It is important to keep these guide rods clean and oiled Protecting Small Fragi2. power switch located directly above the line cord on the rear panel The display will now illuminate indicating that the power connections are correct The display will indicate POWER FAIL this is normal as the pump indicates on the display if power was disrupted since last use The procedure for loading syringes is the same for all models of the Pump 11 Location Requirements for the Syringe Pump A sturdy level clean and dry surface Minimum of one inch 2cm clearance around the pump e Adequate power supply Room temperature 4 C to 40 C 40 F to 104 F e Relative humidity 20 to 80 A well ventilated room Loading the Syringe 1 DY v Abs ND Release the syringe pusher by pressing the bronze button on the side of the pusher While holding the bronze button in slide the pusher to the left Raise the spring loaded syringe retainer and swing it out of the way Lay the loaded syringe in the V shaped holder Swing the retainer so it holds the syringe in place Move the pusher so it makes contact with the syringe plunger Harvard Apparatus Pump 11 VPF Syringe Pump Fuse Replacement Fuse Replacement Make sure the power cord is disconnected from the main supply before servicing the fuse Fuse Replacement Procedure Turn off power remove power cord from power module Use straight blade screw driver pry open access door Remove fuse holder remove fuse s from holder Replace f
3. with leading zeros converted to SP characters ACSII 32 decimal The fifth character is a decimal point ASCII 46 decimal Queries with string response RNG Returns range message character string either ML H ML M UL H UL M Error responses CR LF CR LF prompt Unrecognized command CR LF OOR CR LF prompt Entered value in out of range 5 Harvard Apparatus Pump 11 VPF Syringe Pump Appendix E Single RS 232 RXD TXD GND GND IN Host Computer Connection Appendix F Dual RS 232 GND m RXD RXD TXD GND GND IN Host Computer Connection OUT Chain Connection to Additional Pump s
4. 11 is designed as a low cost highly precise single syringe infusion pump capable of low to moderate back pressures The pump comes in two versions one to hold one syringe of any make from 0 5 pl to 60ml and a second to hold two syringes of any make from 0 5 pl to 10 ml The diameter of the syringe s is entered via the keyboard and the internal microproces sor drives a precision stepper motor to produce accurate fluid flow Non volatile memory stores the last syringe diameter and flow rate along with other configuration data The Power Failure Mode can be set to either turn the pump off in the event of power failure or to resume pumping when power resumes Features Bright Display and Easy To Use Interface A new two line 16 character vacuum fluorescent display along with six membrane keys make this a most attractive but powerful easy to use syringe pump Only two entries required to start pumping syringe Inside Diameter mm and pumping flow rates The Flow rate can be changed while the Pump is running Two Modes of Operation Constant Flow Rate and Volume Dispense The new Pump 11 will operate continuously in RATE mode or accurately dispense a specific amount of fluid in VOLUME mode Smooth Flow New micro stepping pump profiles deliver very smooth and consistent flow Nonvolatile Memory The pump remembers its last syringe size flow rate used and configuration settings Universal Power Supply No need to change AC
5. 50 pA Typically lt 0 05 ohms 95 240 VAC 50 60 hz 15 W 0 25 A fuse 11 4 x 22 9 x 11 4 cm 4 1 2 x 9 x 4 1 2 in 2 1 kg 4 6 Ib Harvard Apparatus Pump 11 VPF Syringe Pump Appendix B Syringe Inside Diameter Terumo Size Diameter 3 cc 8 95 mm 5 13 00 10 15 80 20 20 15 30 23 10 60 29 10 Sherwood Monoject Plastic Size Diameter 1 cc 4 65 mm 3 8 94 6 12 70 12 15 90 20 20 40 35 23 80 60 26 60 140 38 40 Popper amp Sons Inc Perfektum Glass Size Diameter 0 25 3 45mm 0 5 3 45 1 4 50 2 8 92 3 8 99 5 11 70 10 14 70 20 19 58 30 22 70 50 29 00 100 35 70 Stainless Steel Size Diameter 8 cc 9 525 mm 20 cc 19 130 50 cc 28 600 100 cc 34 900 Becton Dickinson Plastic Plasticpak Size Diameter 1cc 4 78 mm 3 8 66 5 12 06 10 14 50 20 19 13 30 21 70 50 60 26 70 Air Tite All Plastic Size Diameter 2 5 CC 9 60 mm 5 0 12 45 10 15 90 20 20 05 30 22 50 50 29 00 Unimetrics Series 4000 amp 5000 Size Diameter 10 ul 0 460 mm 25 0 729 50 1 031 100 1 460 250 2 300 500 3 260 1000 4 610 SGE Scientific Glass Engineering Size Diameter 25 ul 0 73 mm 50 1 03 100 1 46 250 2 30 500 3 26 1 0 ml 4 61 mm 2 5 7 28 5 10 30 10 14 57 Hamilton Microliter Series Gastight Size Diameter 5 yl 0 103 mm 1 0 1457 2 0 206 5 0 3257 10 0 460 25 0 729 50 1 031 100 1 46 250 2 3 500 3 26 1 0 ml 4 61 mm 2 5 7 28 5 10 3 10 14 57 25 23 0 50 32 6 H
6. Special Model 11 Syringe Pump Series User s Manual Model 11 Syringe Pump Series with Serial Communication HARVARD APPARATUS WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will compl
7. Y OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If prac tical enclose a sample or drawing We offer a complete reconditioning service CAUTION This pump is not registered with the FDA C A U T I and is not for clinical use on human patients FOR RESEARCH USE ONLY NOT FOR CLINICAL USE ON HUMAN OR VETERINARY PATIENTS s Harvard Apparatus Pump 11 VPF Syringe Pump Introduction Theory of Operation The new Pump
8. arvard Apparatus Pump 11 VPF Syringe Pump Appendix C Flow Rates Min 2ul 10 ul 50 ul 250 p iml 3 ml 10 ml 30 ml nominal diameter mm 0 10 0 21 0 46 1 03 2 30 4 61 8 66 14 57 23 10 35 00 0 0014 0 0061 0 0289 0 1448 0 7218 2 900 10 24 28 97 72 81 167 2 22 35 98 58 473 0 2 371 9 999 9 999 9 999 9 999 9 999 9 999 0 0001 0 0002 0 0005 0 0025 0 0121 0 048 0 1706 0 4828 1 214 2 786 0 3725 1 6430 7 883 39 52 197 0 791 8 2 794 7 909 9 999 9 999 0 0001 0 0001 0 0001 0 0002 0 0008 0 0029 0 0103 0 0290 0 0729 0 1672 0 0228 0 0985 0 4730 2 371 11 82 47 50 167 6 474 5 1 192 2 738 ml min 0 0001 0 0003 0 0001 0 0016 0 0001 0 0078 0 0001 0 0395 0 0001 0 1970 0 0001 0 7918 0 0002 2 794 0 0005 7 909 0 0013 19 88 0 0028 45 64 Harvard Apparatus Pump 11 Syringe Pump Appendix D Serial Communication Commands Queries and Responses After each transmission to the pump terminating with a CR character ASCII 13 the pump enters remote mode and responds with the three character sequence CR LF prompt The prompt character indicates the status of the pump as follows prompt _meaning ASCII code When stopped ASCII 58 decimal gt When running fo
9. c RUE RETE Ree 11 B Table of Popular Syringe Diameters eee 12 C Table of Minimum and Maximum Flow Rates 13 D Serial Communications Commands Queries and Responses 14 15 E Serial Port Connections Single RS 232 sss 16 Serial Port Connections Dual RS 232 sse 16 Harvard Apparatus Pump 11 VPF Syringe Pump General Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency While the flow will stay calibrated the peak will vary Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCI DENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclu sion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF AN
10. d V keys are used to scroll to the desired number and the SET key moves the underline cursor one place to the right Once the desired diameter is displayed press the ENTER key to place this value into memory Enter Flow Rate Range Choose your flow rate units either microliters or milliliters per minute or per hour From the initial RATE VOL CONFIG menu using the A or V key move the underline cursor under the CONFIG mode menu prompt Press the SET key to enter the CONFIG mode Press the SET key again to move the under line cursor to the flow rate choices Choose your flow rate units while in the SET UNITS mode by pressing the A or V keys to scroll the four flow rate choices ml min ml hr ul hr Once the desired flow rate units are dis played press the ENTER key to return to the main SET CONFIG mode Press SET or ENTER again to put your desired flow rate units into memory and return to the RATE VOL CONFIG menu Enter Flow Rate From the initial RATE VOL CONFIG menu press the SET key to enter the SET RATE mode Each time you change the syringe diameter the previously used flow rate is erased If the syringe diameter is unchanged the previously used flow rate will appear on the display The underline cursor will appear under the left most digit or decimal point The A and V keys are used to scroll to the desired number and the SET key moves the underline cursor one place to the right Once the desired rate is displayed press t
11. e display is to be changed to move the decimal point and to move between modes Each time the set key is pressed the underline cursor below the digit or character on the display moves one step to the right It is used in conjunction with the ascending and descending keys When it the display shows the desired the correct value the set key will advance right to the next digit AV The ascending and descending keys are used to change the numbers on the display A Up key makes numbers increase V Down Key makes numbers decrease When the underline cursor is placed below the decimal point the AV keys shift the decimal one place up or down Run Indicator When the pump is running the highly visible green LED above the RUN STOP key will illuminate Harvard Apparatus Pump 11 VPF Syringe Pump Operation Getting Started 3 5 6 Enter Syringe Size Enter the inside diameter ID of the syringe you wish to use Units are in mil limeters mm If you do not know your syringe diameter refer to appendix B for nominal inside diameters of most popular syringes For the greatest accuracy or if your syringe is not listed in appendix B measure the inside diameter with a vernier caliper or other precision measuring tool Record this value for future use Press SET followed by the DIAM key The previously used diameter will appear on the display The underline cursor will appear under the left most digit or decimal point The A an
12. he ENTER key to place this value into memory Press RUN Press the RUN STOP key to start pump and begin pumping The Run Indicator Green LED above the RUN STOP key will light when the pump is on and pumping Check Syringe Often The Pump 11 will not shut itself off when the syringe is empty or is otherwise overloaded It is prudent to check the syringe from time to time Harvard Apparatus Pump 11 VPF Syringe Pump Operation Advanced Features 1 Volume Mode The Pump 11 can be set to dispense a precise volume and then stop To acti vate the volume dispense mode a target volume must be set To set a target volume at the RATE VOL CONFIG display move the under line cursor using the A or V key to VOL Press the SET key to enter the VOL set mode Use the A or V key and the SET key to display a target vol ume from 00 01 to 99 99 Volume units are either ml milliliters or pl microliters Target volume units are established in the CONFIG SET UNITS mode example if your pumping units are ml min or ml hr then the volume dispense units will be ml s Press the ENTER key to select the desired target volume Exit the VOL mode by pressing the ENTER key Once you press the RUN key the pump will run until the target volume is delivered The display will show the actual volume dispensed along with the target volume Press the RUN key each time you want to repeat the volume dispense If you press the STOP key during a volume di
13. le Syringes The Pump 11 will hold microliter size syringes down to 0 5pl size These small syringes have fine wire plungers that may be damaged if allowed to bot tom out The Pump 11 is equipped with an adjustable stop collar on one of the guide rods Use the provided hex wrench to position and lock the stop col lar to prevent damage to small syringes J Harvard Apparatus Pump 11 VPF Syringe Pump Appendix A Pump 11 Specifications Type Syringe Size for Pump 11 with Holder For 1 Syringe 2 Syringe Flow Rate Range Minimum Maximum Calibration Display Nonvolatile Memory Maximum Force Maximum Pressure Drive Motor Step Rate Minimum Maximum Pusher Advance Step Pusher Travel Rate Minimum Maximum Dynamic Range Leakage to Ground Ground Resistance Voltage Range Power Size HxWxD Weight Microprocessor single or dual syringe infusion only Plastic or glass From 10 yl to 50 60 ml From 0 5 yl to 10 ml 0 0014 ul hr with 0 5 ul syringe 26 55 ml min with 50 60 ml syringe Automatic enter syringe size up to 35 mm Inside Diameter 2 line 16 character vacuum fluorescent display and green run led Stores diameter rate and configuration settings 8 kg 17 Ib 8 p s i with 50 60 cc syringe 100 p s i with 1 ml syringe 0 9 step angle motor 1 pulse in 27 6 sec 200 steps sec 0 8 um pusher advance per motor step 0 7 15 mm min 1 to 16 384 Typically lt 1
14. line switches fuses or wires the new Pump 11 will operate on any AC line voltage from 90 VAC to 240VAC either 50 or 60 Hz Power Fail Mode In a power failure the Pump can either RESUME or STOP pumping when power is returned CE Mark Approved The new Pump 11 meets all relevant European EMC and Safety requirements for laboratory equipment E Harvard Apparatus Pump 11 VPF Syringe Pump Pump 11 Front and Rear Views PUSHER BLOCK E ALLEN HALF NUT WRENCH YN RUN LED ft N E msn O S A Y set diam fenter es ARVA Pump 11 APPARATUS KEY PAD SYRINGE CLAMP SYRINGE HOLDER i ADJUSTABLE STOP E CAUTION an RAT NOT FOR CLINICAL USE ON HUMAN PATIENTS POWER POWER CORD SERIAL SWITCH RECEPTACLE LABEL E Harvard Apparatus Pump 11 VPF Syringe Pump Installation Initial Set up 1 Read the manual to become familiar with all features and functions of the new Pump 11 Pumps are shipped from the factory with an American line cord with a U S three wire molded power plug on one end and a IEC 320 C13 connector on the other Use only an approved AC line cord with a molded IEC 320 C13 connector Turn on main
15. rward ASCII 62 decimal When stalled ASCII 42 decimal Pump 11 does not include stall detection Serial commands and their meanings Commands KEY Return to keyboard control Exit remote mode RUN Start infuse forward direction STP Stop motor CIV Clears volume accumulator to zero CLT Clears target volume to zero dispense disabled Commands with numbers MMD number Set syringe diameter units are mm Rate is set to zero after MMD command ULM number Set flow rate and range units are microliters per minute MLM number Set flow rate and range units are milliliters per minute ULH number Set flow rate and range units are microliters per hour MLH number Set flow rate and range units are milliliters per hour MIT number Set target infusion volume units are ml or pl depending on range Numbers can be between 0 and 1999 Leading zeros and trailing decimal point are optional Any number of digits to the right of the decimal point may be transmitted The number will be rounded Harvard Apparatus Pump 11 VPF Syringe Pump Appendix D Serial Communication Queries DIA Returns diameter value units in mm RAT Returns rate value set in current range units VOL Returns current accumulated infused volume units in ml or pl depending on range VER Returns model and version number of firmware TAR Returns target volume units in ml or pl depending on range value format nnnn nnn The returned value is an 8 character string
16. spense you can restart the pump at the place you stopped by pressing the RUN key again To exit the volume dispense mode set the target volume to 00 00 or turn off and on the pump via the main power switch NOTE In the event of a power failure the actual dispensed volume and the target volume are not retained in memory This means that while in volume dispense mode if a power failure occurred the pump would not resume volume dispense pumping even if the POWER ON mode was set to run Power Failure In the event of a momentary or prolonged power failure the Pump 11 can be set to either a Resume pumping when power is returned with POWER FAIL on the display b Not start pumping when power is returned with POWER FAIL on the display To set the power fail mode at the RATE VOL CONFIG display move the underline cursor using the A or V key to CONFIG Press the SET key to enter the CONFIG mode options Use the A or V key to scroll the CON FIG options until you reach the SET POWER ON display Press the SET key again moving the underline cursor to the right Use the A or V key to scroll the POWER ON choices stop or run Press the ENTER key to select either mode Exit the CONFIG mode by pressing the ENTER key again and save the POWER ON setting in memory Harvard Apparatus Pump 11 VPF Syringe Pump Operation Advanced Features 3 5 Display Intensity For varying light conditions four levels of
17. use s and fuse holder Replace holder with correct voltage marking is showing through access door window For continued fire protection replace fuse only with 250V fuse of the specified type and rating T 1 4 A 250V Fuse not replaceable by the operator is located on the power supply Fuse F 2 0A 250V Screw Driver Fuse Fuse Holder amp A 0 aT Mai i 0 0 M J WARNING USE IN MANNER NOT SPECIFIED BY THE MANUFACTURER MAY IMPAIR THE PROTECTION PRO VIDED BY THE EQUIPMENT 2 Harvard Apparatus Pump 11 VPF Syringe Pump Operation Getting Started Getting started 1 N Turn Pump ON Turn on power using the switch on rear of the pump the display will light and indicate POWER FAIL this is normal as the pump indicates on the dis play if power was disrupted since last use Function Keys and Run Indicator Refer to the colored keypad at the front of the pump to identify the following functions starting from the right run diam stop enten RUN STOP This turns the pump motor on and off ENTER This key enters the data that is on the display into the memory of the pump Also used to query the flow rate DIAM Used to enter or query the syringe diameter SET This key is used to select which digit of th
18. y with any changes as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required E Harvard Apparatus Pump 11 VPF Syringe Pump Table of Contents UBJECT PAGE NO Tabl e of COM tents cue oe eue er Ep E Due ede met ia 1 General Information Warranty and Repairs eee 2 Introduction vi eet n ttr ED RAT ERR Ste 3 Theory of Operation ee hee ette ded tes 3 P at res NR epus 3 New Pump 11 Front and Rear Views esseeeeeeee 4 Installation naeh eae tet e RH 5 Initial Setup amp Location Requirments eere 5 Loading the Syringe neo iter nett inepte ns 5 Fuse Replacement 52 2 pependit 6 BC 7 Getting Started Re dee dte ieu imb 7 Jurn Pump 7 Function Keys and Run Indicator sss 7 Entering Syringe Size ui e on ata eerte 8 Entering Flow Rate Range eren 8 Enterihe Flow Rate i tea aol rete ts 8 Press M M M 8 Ghi ck Syritige iit te nha aves 8 Advanced Features xag 9 Volume Moder suit Rte i ee eoe 9 Power Fail r ett att ie teri ATE RT ve 9 Display Intensity x c e eese 10 Changing Rates cgit nte HO DIRE ne E ree 10 Maintenance eerte ai RE ei teo ns 10 Protecting Small Fragile Syringes sss 10 Appendices AS Specifications cci etre e
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