User Manual
Contents
1. MITES ELEM MID ML RUE d E IE UN dis oe NE MEL IMEEM LEE E ii i n EE rl LE e Place the dispensing bottie to the side of the QFill2 e Screw the dispensing bottle lid onto the bottle ensuring a close airtight fit e identify the stainless steel pipes on the top of the bottle The long angled pipe is the liquid outlet the short straight pipe is the air pressure inlet Liquid Outlet pipe Pressure Inlet pipe_fl a Rubber Washer Figure 3 Bottle cap showing Inlet and Outlei pipes e You need two silicone tubes which are supplied with the OFill2 e Connect the first silicone tube to the Whatman Non Return filter which in turn is connected to the short air pump outlet pipe at the back of the QFill2 Air Pump Outlet Figure d Rear view of QFif2 e Connect the first silicone tube to the QFill2 manifold the steel tube with either 8 or 16 nozzles and then to the long angled stainless steel pipe on the dispensing bottle e Insert the first silicone tube into the pinch valve This is done by depressing the black button of the pinch valve and sliding the silicone tube into the slot making sure that it is fully home e Connect the second silicone tube to the air pressure inlet pipe on the dispensing bottle lid This tube will pressurise the bottle so it must be airtight ed dl ge i es Bs eee CRT n rnm Teg o0 Ae Tar Oe oe ie PG a LAF LES makan m4 Important Double check that the tubing runs through the centre2. Dispensing Volume E Electrical safety Electrical supply Battle anan 4 6 7 3 9 10 T pee tee ea a EELE d EEE TE E drag Ethanol F Filling the dispensing Bottle G CGenetiX 2 00 0cccceepecsscsuress I Isolator switch L LCD Display Liquid spillage M Maintenance Manitold Manual Fill Media ss F hh kgmhhkuwkhnumruz IYAP hn h I FZDBENNMNMENNMREEENNMMMMMMMMMMMMMNKNMMMNENN 19 13 i8 19 adig enren 4 5 6 12 13 9 1 2 4 5 6 7 14 19 eos dl 4 7 5 13 19 od 8 6 7 8 95 12 13 18 19 l4 9 13 18 wee 14 Index Microtitre plates u s nan nway naa nasanaanaaaaawassasanasasasa D O Operating environment eere 2 P Pinch valve wie 4 amp 9 18 19 Plate holder eceeeereees 13 14 Power outict maana F Power switch pamana asaansa naasa 1D Pressurisation KAG 11 Purge Dutton cess eececeeteeareees Lenses 10 i2 Purge tank u s 4 6 1 10 12 19 Purging the System eeeseee eee 10 I2 R REMOVE Lesser tanien rrt rsen 4 9 Reset Dutton 00ecccccsarsccsceceusrareasreasvaucss 11 T RR 10 12 13 8 S AA M AA Ga 4 set Defaults PA 14 Set Positions 20 cee aasawa naaa JA Spare ParmS sees eene rne nan annna rani esons 2D Spillage 2 ceesesescceaeeseseesescennaneeueetsneasaeucuteeuesueass 4 7
3. T Troubleshooting eeeeerneeeee 14 IB F Arr MERE Yo lUM Ee eere nete nnnm rre s 6 i 12 13 4 Warning label aming labels 0 0 0 ec csssseeseeerestesecsersersersarsssesses 3
4. Tube in manifold may be blocked Manifold not dispensing e Tubing has been left in pinch valve after a previous run and now has deformed Fluid continually flows from manifold e Make sure silicone tube is fully inserted into pinch valve Will not pressurise e Check top of media bottle is secured e Tubing is not connected to rear of QFill2 e o ring s perished s Tubing fatigued e Bottle washer perished WellPlate lids jam diring operation e Switch machine off push down lever then remove lids Switch machine on and RESET Stacker fails to stop at end of test e Check that small metal lever in cassette hole has returned to its up position Plate s jammed in stacker e Switch off machine Do not try to pull cassettes stacks out of machine Push down lifter and remove cassette Clear jammed plates replace cassette and Switch machine back on RESET QFill2 Plates s jammed in stacker on entry or exit e Check that stacker height is 2mm lower from QFill2 than QFill2 Pusher in stacker misses plates e Damaged well plates e Pusher bent e Damaged cassette preventing plates from dropping down Stacker stops for no apparent reason e Press START button e Plates may be stuck in cassette e Flag jammed in UP position ey Plates not moving freely up and down in e Cassette feet may be bent stacker cassette Media splashes out of wells when filling Angle the manifold slightly to avoid an air pocket forming
5. Voltage is fixed at either 110vac or 240vac depending upon geographical market Make sure your QFilD is set for the correct supply voltage before attempting to connect to a power supply European Voltage AC210 250V 45 65 Hz single phase Max Current 250mA Power 50W Connections EC inlet USA Japan Voltage AC 90 120V 45 65 Hz single phase Max Current 500MA Power SOW Connections IEC inlet Health 4 Safety Note Before using the QFill2 please make sure that vou have read the manual and comply with the following safety directions Prior to using the QFill2 please make sure that you have been through the QFill2 Pre Power Up checklist to ensure that the QFill2 is set up correctly Safety Features Isolator Switch Located at the back of the machine this turns the power on or off The LCD panel indicates whether or not the power is on Start Stop Located on the keypad pressing this during operation will interrupt a fill routine nuu rema ee yea cesa Aa a anang ze RA I The machine is marked with hazard warning labels All warning labels must be observed Non Return Filter Valve The Whatman Filter valve is fitted on the rear port of the machine and will prevent fluid entering the machine in the event that the tubes are fitted the wrong way round If the Whatman Filter valve becomes wet or damaged it must be replaced Electrical safety The QFill2 must be connected to a properly earthed po
6. at the bottom of the well General Maintenance Note Always switch the machine off and remove the plug from the electricity supply before attempting any maintenance task There is very little in the way of routine maintenance that The Genetix QFiil2 requires The components have all been designed with very long mean time between failures and it is not anticipated that regular replacement of mechanical or electrical parts will be required for many years Cleaning the Machine After operation flush the system through with clean water and drain the dispensing bottle of any remaining liquid Replace the cap on to the bottle and purge the system with air only This 1s to remove any residual liquid from the tubing and the manifold Failure to carry out this operation may result in blocked tubes on the manifold Lastly switch the unit off and remove the silicone tubing from the pinch valve The bottle and cap silicone tubing manifolds and purge tank are autoclavable To clean the manifold more thoroughly use the special screw supplied to pull off the manifold plugs at either end of the large tube They seal the tube with an o ring and may be simply pressed back into place once cleaning is complete Cleaning QFill2 Components It is important that after a filling session has been completed the medium handling elements of the Fil 2 are thoroughly cleaned with 70 ethanol manifold tubing dispensing bottle and plate carri
7. Certificate aa Appendix B Calibration information esee Appendix C OFiH2 Packing List iieeeeeeeeee eee eene nne pa Index 22 22 24 sa 2d 25 25 27 Introduction Genetix QFili2 The QFill2 is an automated QPlate 384 well plate dispenser It is the ideal choice for filling the Genetix range of 384 or 96 WellPlates With this equipment you eliminate the faborious task of manual well filling The QFill2 is ideal for microbial and cell culture applications or for aliquoting solutions in multi well plate assays such as plasmid mini preps or clean up procedures The QFill2 can fill 96 384 and 96 deep wells in less than 20 seconds Before using the QFill2 it is very important that you carefully read this manual and take notice of any safety instructions The QFill2 set up procedure must be adhered to This procedure ensures that the few adjustable parts on the QFill2 are correctly situated in their datum positions Failure to comply with this procedure can damage the Genetix QFill2 Note The Genetix QFilil2 is strictly for research use only and is not intended or recommended for the diagnosis of disease in humans or animals Technical Specifications QFill2 Manufactured by Genetix Ltd UK Dimensions Total assembled Size 30cm x 30cm x 20cm Weight 5Kg Operating Environment Temperature 10 C to 35 C Humidity 20 to 80 non condensing Electrical Supply
8. User Manual QFill2 Copyright 2000 by Genetix Lid All rights reserved No part of this publication may be reproduced stored in a retrieval system or transmitted in any form by any means electronic mechanical by photocopying recording or otherwise without the prior written permission of Genetix Ltd Information furnished by Genetix Ltd is believed to be accurate and reliable however no responsibility is assumed by Genetix Ltd for its use nor for any infringements of patents or other rights of third parties which may result from its use No license is granted by implication or otherwise under any patent rights of Genetix Ltd Revised February 13 2001 Genetix Ltd Queensway Stem Lane New Milton Hampshire BH25 SNN Tel 44 0 1425 624600 Fax 44 0 1425 624700 Email sales genetix co uk Web http www genetix co uk Contents introduction 4 T2021 807 01 LEBER E Technical Specifications t N Ka Heaith amp Safety Note NNNM Safety Features NM Electrical safety NENNEN Drive d NNNM PANA Cleaning Machine cs enne tenerent enne nnn enne rnnt ene retener tetra inen Service and Maintenance aabaana wap apa pO rnm enne PINK ALONA NAA NAGANA NANANG ANA ANA ete ran tnter nnno CA RA dm du Da bo e Initial Operation System T c RR UUERD 6 Pre Power Up Checkitst amaya Power Up Procedures ul ann www anO PAANAN PAALALA NG ANG NANANG KA NN niter na teeth stets pee tran
9. aning purposes When finished press the STOP key and empty the purge tank Preparation Fit the Silicone Mat v 07 ALP LALLE a ager ge Ba ue ee meret e The silicone mat included in the packaging has moulded pegs on its underside e Place the mat so that it aligns with the edges of the moving Plate Holder e Make sure that the pegs are pushed firmly into the holes of the Plate Holder e Three quarter fill the dispensing bottle with the required media for the run e Fit the correct manifold for the type of plate you are using 8 way for 96 well plates 16 way for 384 well plates e Purge the system see page 12 Automatic Filling Place the first plate in the plate carrier making sure it is properly seated on the silicone mat e Ensure that you have keyed in the required volume e Press the START key and the carrier will automatically advance the wells will be filled and the plate will then return to its start position To abort the operation at any stage press the STOP key Dispensing will pause and the display will prompt you to press STOP to end or any other key to continue From time to time the carrier may pause and the message waiting for vessel to pressurise will be displayed This is normal Once the vessel has re pressurised the plate will advance and continue to fili Cleaning after use The QFill2 should be cleaned after each use refer to Cleaning Machine in the General Maintenance section pa
10. bing manifolds and purge tank are autoclavable To clean the manifold more thoroughly use the special screw supplied to pull off the manifold plugs at either end of the large tube They seal the tube with an o ring and may be simply pressed back into place once cleaning is complete Service and Maintenance We would strongly recommend that maintenance be carried out regularly and by a Genetix approved service engineer f you move the system repack it in the packaging material and boxes Hf the QFilD needs to be returned to Genetix under warranty the machine must he returned in its original box to protect it during transit Please ensure that the Decontamination Certificate located in Appendix A is completed and that reasons for return are clearly stated The Product Return and Decontamination Certificate parts I and H must be returned with the machine Initial Operation System Features The Genetix QFill2 consists of the following e Base machine of approximately 30 centimetres square in size se Pump running at approximately 3 5 psi e Pinch valve which controls the working volume of medium in user defined quantities dispensed by the machine e Motorised table which holds a 384 or 96 well microtitre plate e Purge tank e Manifolds 16 way suitable for 384 well microtitre plates and 8 way manifold suitable for 96 well microtttre plates e Pressurised dispensing bottle with silicone tubing e Manual e Bottle holde
11. er Failure to attend to this will lead to blocking of the manifold needles resulting in erratic and inaccurate dispensing of medium If this occurs the components are best cleaned in an ultrasonic bath filled with ethanol and the manifold needles cleaned with fine stainless steel wire Note If the manifold is dismantled check the condition of the o rings because they may perish after cleaning Recommended Spare Parts Spare Parts List for QFill2 Code Description X3172 8 Way Manifold X3173 16 Way Manifold X3174 500ml Bottle amp Cap X3175 Silicone Tubing per metre X3176 Purge Tank X3177 1 Litre Bottle amp Cap X3178 2 Litre Bottle amp Cap X3182 3 Deep 8 Way Manifold X3183 Deep 16 Way Manifold Appendix B Calibration Information bf Q FILL 2 SERIAL No VALUE Settings Adjustment DEFAULT CALIBRATION 4 litre 1 litre 17 0016 b jt a HL DW 0200 aso Ae P 080 geg So 20 Coarse 6011 ji 96 plates Fine LES il o T eo Solenoid Tan The table shows a record of the calibration settings specifically for the QFili2 whose Serial Number appears in the box above If you find it necessary to modify or change these settings please contact Genetix Ltd for instructions Please Note changing these settings without supervision by Genetix personnel could affect your warranty B Bath C Change Volumes Cleaning eese D Dimensions
12. ge 20 Menu Options To access the menu options press the MENU key There are 4 available options Fev nena PHE npn oe kaaa aah This allows you to edit the 16 pre set volumes Use the a v keys to move to the memory position you wish to change To edit a certain volume press the ENTER key and then adjust the 4 digit value using the a v keys The editor works in a similar way to a combination lock Each digit may be rolled up or down by one unit The ENTER key advances to the next digit Once you have modified the volume press ENTER Press menu to return to the menu screen and menu again to return to the opening screen To confirm the volume change press the VOL key scroli to the volume you have just altered and press ENTER The QFili2 always displays the last volume used SET POSITIONS Warning changing these settings may affect your Warranty This option allows the pre determined plate and well co ordinates to be altered and is not required for normal filling operations The values are pre set by Genetix personnel and should only be changed by Genetix engineers for troubleshooting purposes MANUAL FILL Having selected the required volume this menu will allow you to step the plate manually one row at a time Scroll to manual fill and press ENTER Place a plate in the plate holder and having selected the desired volume Press the v key to dispense the liquid and the 4 key to move to the next row Press STOP twice at a
13. he QFill2 the power supply should be disconnected at the mains before any attempt is made to clean up the spillage e Connect the power cable for the QFilf2 to your power source The QFill2 uses a stainless steel manifoid with either 8 or 16 nozzles to dispense liquid evenly into wells 8 way manifolds are used for 96 WellPlates and 16 way manifolds are used for 384 WeliPlates Standard manifolds are designed for Genetix plates but custom types are available for deeper well plates To fit the manifold insert the tapered end of the manifold back plate see Figure 2 on page 8 into the black slot in the centre of the QFill2 unit The machine will automatically detect whether an 8 or 16 way manifold is fitted by the hole in the back plate An 8 way manifold has the hole on the left see Figure 2 and the hole is on the right hand side of the back plate of a 16 way manifold Tapered end wm e e eo ieg of manifold back plate Figure 2 S way Manifold hole in back plate on left hand side For accurate filling the manifold must be purged of air first See the section on purging page 12 Connecting the dispensing bottle A iai Pk RR n l aun u H CHE HP AYU ie h CH KC a a Aa RG HA 9 sara ae a 83 eam pe o ss so er Sage sge Jo GS f Important Please make sure that you follow these instructions correctly Connecting the tubes wrongly will render your warranty null and void
14. ins power to the QStacker Switch on the QFill2 then the QStacker Press the RESET key on the QFill2 to make it home The END position for the QFill2 carriage should be preset by Genetix so that it aligns with the manifold The START position is preset by Genetix so that the gap between the base plate and the QStacker extension is about 3 mm Figure 8 As the carriage moves to the left to pick up a WellPlate the pin on the base plate strikes the clamp activator bolt on the QStacker Figure 7 causing the plate retaining clamp to open Adjust the clamp activator bolt so that the plate retaining clamp opens sufficiently to allow plates to move onto the base plate from the stacker Connector Plate Base Plate Carriage Figure 6 Side View of QFill2 Side Plate Connector Plate QStacker GFill2 Clamp activator bolt Carriage Extension Figure 7 fop view of OStacker and QFilI2 prior to connection Approx 3 mm gap Figure 8 Alignment of extension to carriage base plate Operating instructions Switch on both the QStaeker and the QFill2 2 Press the RESET key on the QFili2 3 Set the required volume to be dispensed 4 Load the WellPlates to be filled into a cassette and fit the cassette into the left hand position of the stacker Note Plates must be loaded with lids on Alternatively plates can be loaded into the cassette after it has been fitted to the machine The cassette will hold a maximu
15. l show the following message GENETIX Ready to RESET Software Version After the QFill2 is switched on press the RESET key This will initialise the drive system and start the pressurisation cycle The boitle must be connected and airtight at this point You should briefly see this display GENETIX Wait for vessel TO pressurise If you do not see the following display within about 30 seconds check that ali the tubes are connected and the bottle top is serewed on tightly GENETIX READY Volume 0050 Plate The dispensing volume and plate type 96 or 384 will be displayed The volume displayed is always the last volume used Setting the Dispensing volume e Press the VOL key to view the volume options e Scroll through the volume options using the 4 w keys e When the asterisk is next to the desired volume press the ENTER key The screen wil now display the chosen volume e To return to the previous screen at any time press the CLEAR key Purging the system The system must be purged of air for accurate filling From the following screen GENETIX READY Volume 0050 Plate type 96 press the PURGE key to see the following screen Press purge or lt stop gt to end At this point press and hold the PURGE key to run liquid through the tubing and manifold and expel any air in the system This function may also be used to flush water through the system for cle
16. m of 40 Genetix WellPlates For ease of application it is better to load all WellPlates in the same direction 5 Fit an empty cassette into the right hand position of the stacker Ensure that the cassette engages witb the clamps 6 Press the START key on the QStacker and the machine will take plates from the left hand cassette to the OFill2 dispense liquid into the plate and return it to the right hand cassette 7 Once all the plates in the left hand cassette have been filled the machine will stop The right hand cassette containing the filled plates can then be removed by squeezing the levers on each side of the base of the cassette and lifting it out of the machine 8 If the STOP key on the QStacker is pressed during a run the machine will finish filling the current plate then stop The run can be restarted by pressing START on the QStacker 9 If the STOP key on the QFilD is pressed during a run the machine will pause until either STOP is pressed or any other key is pressed to continue Troubleshooting Common Problems Problem Possible Cause Solution Air bubbles blow through the dispensing Check that silicone tubing is connected to bottle liguid the correct tubes on the cap e Check that tube is fitted in pinch valve correctly Leakage from manifold e Check for leak s in silicone tube Low manifoid nozzle pressure e Tighten bottle cap e Check that silicone tube is not blocked Not all tubes dispensing e
17. ne 10 Running a Filling Routine 11 Initialising the machine oe eee eee cette cere tage tenete etre senem eer eene senescensees EE Setting the Dispensing volume eseiseseeeese eene nnne nne ne nione nitet ene taris erratean ED Purging the System Lpg pana pasan sawaan nanetnnbr te nemen eerte nte enses BA Preparation eieseiseeseeeseeeeeenenesenmettneetnettetet ened nL et ema niet a menter enr cn cnet ies NI Automatic AK Cleaning after use isses nemen rat rennen teer tee nhan enenatis BO Menu Options many sama nana aaa unG NOO AGUA NO A04 NB rta KG sues tnnt ah KANAN eene en aee nee rne nn eeneeeanen rn j4 QStacker 15 Assembly Instructions CLR t er tt eee eee re Lee ee ee es ee ee ee ee eee eee ee o d mah om 15 Operating Instructions rhuwhpnuimmuuamnukuzarimhHik J RR AWEACRATARU SRORnaX4RER A RtkumakoadhyFsdapsRA3ERABISEFAIALRGLBALBRAG RR wd kd nu ha I7 Troubleshooting 18 Common Problems saessesesisrisareeantinrrnirenrearereararrnnneerannnea estes FS General Maintenance 20 Note mdagagp magada erap ara paaa bga hara aa AA 20 ARAERERREBEREEFNaRddnadhkhahswandhsqzuhcquRgooey den Cleaning the Machine jad cuaestavasauauenseccdacsuausaueeepeedus avacsetsuscessucuesanetesetecetscencetccce 20 LGE wo nm al Cleaning QFill2 Components EE VENERE 20 Recommended Spare Parts 21 Spare Parts List for QVFilI2 esesieesses secessit enitn reee recede niece ecce Appendix A Product Return and Decontamination
18. ny time to abort the fill Warning changing these settings may affect your Warranty This option also refers to plate and well co ordinates which do not require adjusting for normal operation It should only be used by Genetix engineers for troubleshooting purposes QStacker Assembly instructions Note Steps 1 3 only apply if you have purchased the QStacker independently l 2 10 11 12 Screw the base plate onto the QFill2 carriage Figure 6 Remove the three screws from the left hand side of the base of the QFiID Align the holes in the connector plate with the holes in the base of the QFilD2 and replace the screws so that the connector plate is attached to the QFill2 with the pillars pointing up Figure 6 Place the QStacker next to the QFill2 so that the slots on the side plate fit over the pillars on the connector plate Figure 7 Push the QFill2 carriage across to the left and adjust the position of the QStacker so that the extension fits into the recess of the carriage Tighten the front pillar on the connector plate The height adjuster screw is located on the side plate Set this so that the extension is slightly below the base plate Place a WellPlate on the base plate and ensure that it can slide smoothly over the extension Connect the power output lead from the QStacker to the power socket on the QFill2 Connect the serial cable on the QStacker to the serial port on the QFilI2 Connect the ma
19. of the pinch valve Failure to do so may result in a spillage and will render your warranty null and void Figure 3 ee eee HHH Tree es bebe timate if you have just connected the bottle according to the instructions above unscrew the bottle now but leave all the tubes connected The easiest way to do this is to hold the top still while rotating the glass bottle Fill up the dispensing bottle with the required media never more than three quarters full It is advisable that the first time you set up the QFHI2 it is done with water Power Up Procedures Switch on the machine The power switch is on the back panel Press RESET on the keypad to pressurise QFill2 Allow the machine to run for approximately thirty seconds in order to build up pressure in the dispensing boitie Press the PURGE key The option to PURGE will be displayed Purging the system allows any air in the tubing to be displaced Press the PURGE key and hold it down unti all the air has been removed Press the STOP key to return to the previous screen Empty the purge tank and replace it on the machine Running a Filling Routine Initialising the machine The QFill2 features an easy to use menu interface on Its integral LCD display Once the unit has been set up and the dispensing bottle filled it is a simple process to fill large numbers of plates with a precisely measured volume of liquid After powering up the QFill2 the LCD display wil
20. r ring e Manifold cleaning screw e Mains Lead Pinch Valve e Silicone Mat inlet tube from short straight pipe to air pump Keypad Outlet tube from angled pipe through Pinch Valve to manifold Silicone Mat Manifold Plate Holder Figure 1 Purge Tank Capacities The QFill2 is normally configured and calibrated ai the factory to work with 0 5 litre and 1 0 fitre bottles If re calibration is necessary refer to Appendix B Note please ensure that the serial number recorded in the manual matches the serial number of the machine because each machine is calibrated individually to ensure optimum performance Pre Power Up Checklist e Check that the master switch on the back panel of the QFill2 is in the OFF position and plug the power cable into the back of the unit Check that the tubing is not fatigued s Make sure that the sealing washer on the bottle cap is not perished e Check the o rings on each manifold plug to make sure that they are not perished e Fasten the botile retaining clip onto the side of the unit using a flat bladed screwdriver to tighten as necessary Place the dispensing bottle inside this clip e Inspect the bottle for evidence of cracks scratches or any other damage e Fit the purge tank onto the front of the QFilD Always make sure that it 1s properly seated down The purge tank is designed to catch any spillage from the dispensing nozzies e In the event of a liquid spillage into the base cavity of t
21. wer outlet to protect users from the risk of electric shock The main chassis of the machine is earthed together with all associated electrical components Do not remove any of the fixed covers as there are no user serviceable parts inside All internal work should be referred to Genetix approved service personnel The QFill2 has a Warranty seal and is marked with tamper evident sealant if any of the covers are removed the guarantee is rendered null and void In the event of a liquid spillage into the base cavity of the QFill2 the power supply should be disconnected at the mains before any attempt is made to clean up the spillage Drive Safety The QFill2 incorporates a moving plate tray Keep objects and fingers clear from this mechanism when the robot is in operation The Pre Power Up Checklist on page 7 should be followed before every routine in order to prevent serious damage to the QFill2 or any of its constituent parts Cleaning Machine The machine should be cleaned after use After operation flush the system through with clean water and drain the dispensing bottle of any remaining liquid Replace the cap on to the bottle and purge the system with air only This is to remove any residual liquid from the tubing and the manifold Failure to carry out this operation may result in biocked tubes on the manifold Lastly switch the unit off and remove the silicone tubing from the pinch valve The bottle and cap silicone tu
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