Clarity Controls Agilent 1100 Clarity Controls Agilent 1100
Contents
1. 41 5 5 7 Hardware Configuration Info 0 41 5 60 MWD GI30S jercrri teira einne RE IS aT 42 5 6 1 Agilent 1100 Configuration MWD 42 5 6 2 Method Setup Acquisition Signal 43 5 6 3 Method Setup Acquisition MWD 45 5 6 4 Method Setup Acquisition Outputs 46 5 6 5 Method Setup Acquisition Time TADS esi dette ae AEE cusectlecs EE E EEEE 47 S7 VWD GISl erer ena aaa i is 48 5 7 1 Agilent 1100 Configuration VWD 48 5 7 2 Method Setup Acquisition Signal 49 5 7 3 Method Setup Acquisition VWD 51 5 7 4 Method Setup Acquisition Outputs 52 5 7 5 Method Setup Acquisition Time TADIE s 5 c coreshabectsee sinapan ienaa 53 5 8 LC Pump G1310 G1311 G1312 53 DSL OPONSE a EET ETE EEEE 57 5 9 Column Compartment G1316 58 5 9 1 Agilent 1100 Configuration AS 58 5 9 2 Method Setup Thermostat Temperature cassiere EErEE E 58 5 9 3 Method Setup Thermostat Time EVENTS oiaren iipasi tse E An E EE 60 O C nnecti hS oheari apaci nio a E va a 61 6l Wining ee oere cand obi doped ia aa EA EE E E aa i eont 61 6 2 Description of connectors ssssseseesererereerererese 61 6 2 1 LAN ift rface mi ae iennige 61 6 2 2 GP IB communication eee 62 6 3 Recommended GPIB addresses eee 62 7 Troubleshooting cece cece eee ee ence S eens 63 C2. Clarity Controls Agilent 1100 LC ENG Code Rev M060 24D 19 October 2007 Phone 420 251 013 400 DataApex Ltd 2007 Fax 4420 251 013 401 Podohradska 1 clarity dataapex com 155 00 Prague 5 www dataapex com The Czech Republic Clarity DataApex and 4 are trademarks of DataApex Ltd Microsoft and Windows are trademarks of Microsoft Corporation DataApex reserves the right to make changes to manuals without prior notice Updated manuals can be downloaded from www dataapex com Clarity Agilent 1100 Table of Contents 1 Agilent 1100 Control Module eee eeeee 5 2 REGUIETEMENTS o niee E eE NEIE EAE Er T 6 3 Installation Procedure esesssesereerrrerererrrrerrereserereeees 7 3 1 Installing the GPIB interface in the PC 0 7 3 2 LAN Card settiNg essiri i sssi sssaad seeds eens iea 9 3 3 Clarity configuration esessssseesereserrereresreresesee 11 3 3 1 Assign the control to Clarity lnstram nt ereere iei E AE EEEa 14 3 4 First start of Clarity after configuration 14 4 Using the Control Module s ssseseeseeseseersrereseerrrereeees 16 4 1 Method Setup AS Control ee 16 4 2 Method Setup LC Control ee 16 4 3 Method Setup Acquisition 0 ee 17 4 4 Method Setup Thermostat ee 17 4 5 Data Acquisition cece eee cece eee eeeeeeeeee 17 4 6 Device MOMItor 0 c cccceeeesceeeeeeeeceeeneeceveeccteenees 1
3. 74 074 Hz flash energy high economy mode flash frequency 74 074 Hz flash energy low Lamp Energy Reference Defines whether the lamp energy signal is used as reference for the fluorescence signal or not The lamp energy signal is measured with reference diode 5 5 4 Method Setup Acquisition Outputs Method Setup PDA_test Agilent 1100 FLD Method FLD Parameters Flashlamp Outputs Time Table Analog output 1 Zero offset 5 1 00 Open Open Open 1000 2 00 Close 3 00 Close Range fiv x 4 00 Tose 5 00 Attenuation mAU Analog output 2 Zero offset 5 1000 Attenuation mA Range V Not Connected AS Control Lc Control Measurement Acquisition Thermostat 1 Integration PDA Method Calculation Cancel Apply Report Fig 26 Method Setup Acquisition Outputs Analog Output x Zero Offset Sets the zero offset voltage for Analog Output 1 2 to a percentage of the full scale voltage 39 Clarity Agilent 1100 Reference description Attenuation mAU Selects analog output attenuation The FLD offers two analog outputs signal A at analog output 1 and signal B at analog output 2 The attenuation can be changed in power of 2 between 1600LU and 0 2LU Range V Sets the output signal Range to 1 V or to 0 1V Table The Output relays on detector are op
4. Slit nm 4 xf Margin for neg absorb m4U JT Run time min JT Post time min Not Connected AS Control IE Control Measurement Acquisition Integration PDA Method Calculation Cancel Apply Report Fig 31 Method Setup Acquisition Signal Detector 1 External Start Stop Enables control through the use of an external event Note The digital detectors distinguish Start and Stop events so it is not necessary to specify the Start Start Start Restart etc options like with the A D converters Range mV Sets the signal range of the detector Sample nm Sets the centre of the wavelength of the detector Bw nm Sets the bandwidth for the sample wavelength in nm 43 Clarity Agilent 1100 Reference description Valid Values 2 400 Use reference unlabeled checkbox Use the reference wavelength to compensate for lamp fluctuations Ref nm Sets the center of the reference wavelength in nm Valid Values 0 190 950 Bw nm Sets the bandwidth for the reference wavelength in nm must be O if reference is switched off 190 lt Ref Bw 2 lt 950 Valid Values 0 2 400 Common Rate Hz Selects the data acquisition rate for the stored signals Autozero Before Run Resets the detector to zero at the beginning of the analysis Autozero After Run Resets the detector to zero after finishing the analysis Slit
5. nm Defines the width of the built in microslit in its open position The instrument performs a zero automatically whenever the Slit parameter has been changed Margin for neg absorb mAU Maximal negative value of absorbance that will be still taken into account Run Time min Run time of the analysis Data acquisition will be finished after the specified time unless a shorter time is specified in another module 44 Clarity Agilent 1100 Reference description Post Time min The system will stay in running state for the specified time after the analysis is finished 5 6 3 Method Setup Acquisition MWD Method Setup DEFAULT2 Select Detector MWD Detector 1 Enabled Agilent 1100 MWD Method Signal Outputs Time Table Lamps set state after send method F UV lamp I Vis lamp check state before run F UV lamp I Vis lamp Not Connected Measurement Acquisition Integration PDA Method Calculation Cancel Apply Report Fig 32 Method Setup Acquisition MWD Lamps Set state after send method Checkboxes UV Lamp Vis Lamp will set the respective lamp ON after sending the method in case it is OFF Check state before run Configure if UV light source UV Lamp or visible light source Vis Lamp is required Determines whether a method requires the selected lamp or not The module becomes NOT READY state if the respective lamp is switched
6. off 45 Clarity Agilent 1100 Reference description 5 6 4 Method Setup Acquisition Outputs Method Setup DEFAULT2 Select Detector MWD Detector 1 Enabled Agilent 1100 MWD Method Signal MWD Time Table Analog output Time Cont Cont Cont cont Zero offset EE min not no 2 no 3 no 4 Attenuation mAU 1000 Range lV 1v Analog output 2 Zero offset Attenuation mA 1000 Range 1V Not Connected Measurement Acquisition Integration PDA Method Calculation Cancel Apply Report Fig 33 Method Setup Acquisition Outputs Analog Output x Zero Offset Sets the zero offset voltage for Analog Output 1 2 to a percentage of the full scale voltage Attenuation mAU Selects analog output attenuation The DAD offers two analog outputs signal A at analog output 1 and signal B at analog output 2 The attenuation can be changed in power of 2 steps between 2000 mAU and 0 98 mAU Range V Sets the output signal Range to 1 V or 0 1 V Table The Output relays on detector are optional feature of certain Agilent 1100 configuration If it is present in your configuration then the table will enable to set the timetable of events to trigger these outputs 46 Clarity Agilent 1100 Reference description 5 6 5 Method Setup Acquisition Time Table Method Setup DEFAULT2 Select D
7. 0 00 C Demo LC Control Measurement Acquisition Thermostat 1 Integration PDA Method Calculation Fig 43 Note Cox Cancel Apply Report Method Setup Thermostat Temperature Flow Compensation Auto x Off x specific flow Column switching valve Sets the column to be used in the current method The column can be switched during the analysis using the timed event see pg 59 Run Time min Run time of the analysis Data acquisition will be finished after the specified time unless a shorter time is specified in another module Post Time min The system will stay in running state for the specified time after the analysis is finished 59 Clarity Agilent 1100 Reference description 5 9 3 Method Setup Thermostat Time Events The Time Events tab is used to display and modify a table with time program of temperatures Method Setup PDA_test Agilent 1100 COC Method Temperature Time Events Temp Right Temp rc Temp rel EoiN 1 00 Change 30 00 Change 60 00 20 00 OFF OFF Not Connected AS Control Lc Control Measurement Acquisition Thermostat 1 Integration PDA Method Calculation Cancel Apply Report Fig 44 Method Setup Thermostat Time Events Time min Sets time when at least one of the temperatures in the table should be changed Left Temp Switches the Left tempera
8. Connections 6 2 2 GP IB communication The HP IB connector is a standard on the Agilent 1100 series modules Fig 45 Back side of Agilent 1100 components General purpose remote cable pin layout and colours Pin HP 1100 Signal Name Active TTL 1 White Digital ground 2 Brown Prepare run Low 3 Gray Start Low 4 Blue Shut down Low 5 Pink Not connected 6 Yellow Power on High 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 6 3 Recommended GPIB addresses Default Addresses Autosampler 28 Autosampler 28 Pump 22 RID 29 FLD 23 VWD 24 Autosampler HP 1050 18 Agilent 8453 25 Pump HP 1050 16 DAD MWD 26 VWD HP 1050 10 Column Compartment 27 DAD HP 1050 17 For GP IB board is recommended to use the Address 21 default or 30 62 Clarity Agilent 1100 Troubleshooting 7 Troubleshooting Problem 1 Communication problem The communication cable must be connected to the module with most extensive communication When DAD _ detector is present it should be used for communication Otherwise first other detectors and only last the other modules should be used Problem 2 Communication problem Communication problems may be caused by GP IB Address conflict on the GP IB bus Each Module including the GP IB board must have unique address The Addressees can be changed for the individual modules by the DIPswitch settings on their backside Default settings are listed in 6 3 Recomm
9. IP address according to the bootp manager settings in Clarity Agilent 1100 configuration too 10 Clarity Agilent 1100 Installation Procedure Note Detailed description of the possible LAN interface card settings can be found in the Agilent G1369A LAN Interface User Manual Agilent P N G1369 90000 3 3 Clarity configuration e Invoke the System Configuration dialog accessible from the Clarity window using the System Configuration command e Press the Add button to invoke the Available Control Modules dialog Available Control Modules EP Detector Agilent 1100 Wizard Bischoff2250 Pump Driver B11 Pump Driver CB20 Pump Driver ECOM LCP 4100 Pump Driver FLUX RHEOS 2000 Pump Driver GILSON 30K Pump Driver LabAlliance Pump Driver UNI Pump Driver AS PDA ff Balance Thermostat Add Cancel e Select the Agilent 1100 Wizard and press the Add button e The Agilent 1100 Configuration dialog will appear If you have LAN skip to LAN communication section GPIB communication e Set the GPIB Board name Note Use the SICL Name set for the board in the Agilent IO Libraries configuration IO Config window e Set the Address according to the BUS Address of the Agilent 1100 component that is physically connected with the PC It is recommended to connect the component with largest amount of communication which is usually DAD detector 11 Clarity Agilent 1100 Installation Procedur
10. detailed description of the individual controls for each dialog of the Agilent 1100 Control module This detailed description is easily accessible in the online help system just press the Fd button while being in the respective dialog 5 1 Agilent 1100 Configuration Common This dialog sets the basic communication parameters of the GPIB interface After the communication with the interface is properly established the Auto Detect button will automatically detect and connect all Agilent 1100 components Agilent 1100 Configuration GPIB Communication Auto Detect Board Address gpibO 1 zi Remove Common VWD FLD DAD Isocratic pump Quart Pur 4 Cancel Help Fig 10 Agilent 1100 Configuration Common It is also possible add or remove components manually However this is recommended only in specific cases and for advanced users Board Sets the GPIB interface name Use the SICL Name set for the board in the Agilent IO Libraries configuration IO Config window See the 3 2 on pg 9 to learn how to correctly set this field 20 Clarity Agilent 1100 Note Reference description Address e Sets the BUS Address for the Agilent 1100 component that is connected to the GPIB cable Default factory settings are listed in chapter 6 3 Recommended GPIB addresses on pg 62 The interface address set for the GPIB interface in Agilent IO Libraries configuration IO Config
11. offered as default Should the setting be compatible with the Chemstation the SICL Interface Name must be HP82341 Write down the SICL Interface Name and BUS Address fields it will be necessary to set these values later also to the Clarity Agilent 1100 Configuration dialog e Connect the Agilent 1100 to the PC by GPIB interface It is recommended to connect the GPIB cable between the PC and the Agilent 1100 component with the largest amount of communication This is usually the DAD detector If it is not present in the set then any other detector MVD WVD FLD etc e Turn the power on both PC and Agilent 1100 e Review the GPIB settings Clarity Agilent 1100 Installation Procedure 3 2 LAN card setting e To operate properly in a network environment the LAN Interface must be configured with valid TCP IP network parameters These parameters are e IP address e Subnet Mask e Default Gateway e There are several different Init modes for setting the IP address of the Agilent 1100 system selectable by dip switch settings on the LAN communication board SW4 SW5 SW6 InitMode OFF OFF OFF Bootp OFF OFF ON Bootp Store on i i 5 i l l I I OFF ON OFF Using Stored 2345678 OFF ON ON Using Default Fig 3 LAN card DIP Switches Default settings e The factory set Using Default option SW5 ON and SW6 ON uses fixed IP address 192 168 254 111 In case the 1100 is connected directly to
12. to specify the Start Start Start Restart etc options like with the A D converters Base WaveLength Sets the base wavelength of the Excitation and Emission for the selected detector Excitation Detector1 Sets excitation base wavelength for detector channel 1 2 3 or 4 Possible options are Zero Order or specifying a wavelength value default is 460 nm Emission Sets Emission base wavelength of the selected detector channel 1 2 3 or 4 Possible options are Zero Order or specifying a wavelength value default is 460 nm PMT Gain Sets the sensitiveness PMT gain of the photomultiplier 36 Clarity Agilent 1100 Note Note Reference description Rate Hz Sets the data output rate of the detector to the workstation The data rate cannot be changed during RUN mode of the detector Multi wavelength Mode Defines the Multi Wavelength Mode of the detector Clarity Control module does not enable to switch the Multi wavelength Mode OFF Switching between different multi wavelength modes lasts from 20 up to 50 seconds the module becomes NOT READY in between Excitation multi excitation wavelength and excitation spectrum Emission will be constant EX 4 EM Emission multi emission wavelength and emission spectrum Excitation will be constant EX1 EM1 4 Spectra Acquisition Set parameters for acquisition of excitation emission spectra depending on the setting of the Mul
13. window should be different from those used for the connected Agilent 1100 components See the 3 2 on pg 9 to learn how to correctly set this field Auto Detect Automatically detects current configuration of the Agilent 1100 set Add Remove Invokes the Add Module Remove Module dialog described in following chapter 5 2 Add Module Adds tabs corresponding to the specific configuration of the Agilent 1100 set to the Agilent 1100 Configuration dialog Add module Module type Serial number G1313 Agilent 1100 AS z o Add Cancel Fig 11 Add Module Module Type Select the desired module type to add a corresponding tab to the Agilent 1100 Configuration dialog Serial Number States the serial number that can be found on a label on the device 21 Clarity Agilent 1100 Reference description 5 3 Autosampler AS G1313 and TAS G1329 5 3 1 Agilent 1100 Configuration T AS Agilent 1100 Configuration Type G1329 Agilent 1100 Sampler Serial number 5646461464 Common DAD RID Quart Pump tas COLCOMP Cancel Help Fig 12 Agilent 1100 Configuration TAS Type Type of Agilent 1100 component indicated by name and code number Serial Number Serial number must correspond to the serial number imprinted on a label on the device 5 3 2 Method Setup AS Control Injection The Injection tab specifies the basic autosampler settings Standard Injection Injectio
14. 9 5 Reference description eee cee cec eect e cece eeeeeeeeees 20 5 1 Agilent 1100 Configuration Common 20 5 2 Add Module neinar ear eeir a aiaee 21 5 3 Autosampler AS G1313 and TAS G1329 22 5 3 1 Agilent 1100 Configuration T AS 22 5 3 2 Method Setup AS Control Injection 22 5 3 3 Method Setup AS Control Program 24 5 3 4 Hardware Configuration AS ee 26 54 DAD G13 V5 sesaciccsskectoategees nogedcevarcecabaetcongesoneonete 27 5 4 1 Agilent 1100 Configuration DAD 27 5 4 2 Method Setup Acquisition Signal 28 5 4 3 Method Setup Acquisition DAD 30 5 4 4 Method Setup Acquisition Outputs 31 5 4 5 Method Setup Acquisition Time Tableiisis chctniioreitsineeatenietcentesaaravoi nets 32 5 4 6 Hardware Configuration Main 33 5 4 7 Hardware Configuration Info 34 D2 FLD GIIDI Jao Aa ates eter aoanane oar EE ead teeansse 34 5 5 1 Agilent 1100 Configuration FLD 34 5 5 2 Method Setup Acquisition FLD PALrAMETELS s cecsccie eve stveoes sun a E aE ETE 35 Clarity Agilent 1100 Table of Contents 5 5 3 Method Setup Acquisition Flashla Mpi smior raires eeni a isas 38 5 5 4 Method Setup Acquisition Outputs 39 5 5 5 Method Setup Acquisition Time LADLE Sabi oa E sev aslo E e EE 40 5 5 6 Hardware Configuration Main
15. Autozero Before Run Resets the detector to zero at the beginning of the analysis Autozero After Run Resets the detector to zero after finishing the analysis Margin for neg absorb mAU Maximal negative value of absorbance that will be still taken into account Run Time min Run time of the analysis Data acquisition will be finished after the specified time unless a shorter time is specified in another module Post Time min The system will stay in running state for the specified time after the analysis is finished 50 Clarity Agilent 1100 Reference description 5 7 3 Method Setup Acquisition VWD Method Setup DEFAULT2 Agilent 1100 VWD Method Signal VWD Outputs Time Table Lamps set state after send method I UV lamp check state before run IF UV lamp Not Connected Measurement Acquisition Integration PDA Method Calculation Cancel Apply Report Fig 37 Method Setup Acquisition VWD Lamps Set state after send method Checkboxes UV Lamp Vis Lamp will set the respective lamp ON after sending the method in case it is OFF Check state before run Configure if UV light source UV Lamp or visible light source Vis Lamp is required Determines whether a method requires the selected lamp or not The module becomes NOT READY state if the respective lamp is switched off 51 Clarity Agilent 1100 Reference description 5 7 4 Method Setup Acqu
16. Clarity Agilent 1100 Caution Caution Caution Reference description See the Agilent 1100 User manual for detailed description Amount ul Specifies the volume used in operation Speed ul min Sets speed for drawing ejecting in micro litres per second This parameter determines how fast the metering device moves during the selected operation Source Displays a list of options Vial Specifies used vial number where appropriate Offset mm Defines the offset of the needle from the default draw position in a vial O means default draw position negative values move needle closer to the bottom of the vial View Copies the content of the selected line to the fields above the table Insert Inserts a line bellow the active line containing the instruction defined in the fields above the table The button is active only when the Injection Program is selected on the Injection tab Change Replaces current line with the instruction specified in the fields above the table The button is active only when the Injection Program is selected on the Injection tab Delete Deletes selected line The button is active only when the Injection Program is selected on the Injection tab 25 Clarity Agilent 1100 Reference description 5 3 4 Hardware Configuration AS This dialog is invoked by pressing the AS Status button in the Method Setup AS Control dialog Hardware Configuration Type of Sampler Agile
17. Compartment G1316 5 9 1 Agilent 1100 Configuration AS Agilent 1100 Configuration Type G1316 Column Compartment Serial number 1 I Column switching valve Common COLCOMP Cancel Help Fig 42 Agilent 1100 Configuration COLCOMP Type Type of Agilent 1100 component indicated by name and code number Serial Number Serial number must correspond to the serial number imprinted on a label on the device Column switching valve Select if the column switching valve is present in the configuration 5 9 2 Method Setup Thermostat Temperature The Temperature tab specifies the left and right temperature settings Temperature left Not Controlled x specific temperature Enable Analysis With Any Temp Temp is in Range Sets the temperature control mode When a temperature range is set the module will be Ready only after the temperature reaches the preset limits 58 Clarity Agilent 1100 Reference description Method Setup DEFAULT2 Common for all detectors Agilent 1100 COC Method Temperature Time Events Temperature left Temperature right Flow compensation Same as lelt Auto C Off Not controlled Not controlled C ml min 20 c c Column switching valve Enable analysis Enable analysis Column 1 1 gt 2 id With any temp gi Temp is in range iD J Run time min 20 08 C Description Ready Temp left 0 00 C right
18. Initial The flowrate is defined by the first row of the gradient table the Initial row Standby The flowrate is the same as in the STANDBY mode and accordingly corresponds to the value entered in Standby Flow 56 Clarity Agilent 1100 5 8 1 Options Caution Reference description The IDLE state enters into effect each time an instrument is opened at the end or after abortion of an analysis by the Abort command and is maintained also when the Clarity program is shut down The mixing ratio of individual components in both the IDLE and STANDBY states is given by the first row of the Gradient Table the Initial row By invoking the Options button in the Method Setup LC Control dialog the Gradient Options dialog will appear Gradient Options Flow Rate Units Solvent 1 Pressure Units MPa zi Vv Min Pressure f0 Vv Max Pressure MPa Vv Cancel Fig 41 Gradient Options In this dialog the Flow Rate and Pressure units can be selected the pressure limits set and Solvents can be enabled and named Do not disable the solvents for binary or quaternary pumps even if you are running isocratically Only the data for enabled solvents are sent to the pump and if the pump remembers non zero percentage for not used solvent from previous method it will not accept the new values since the total would exceed 100 57 Clarity Agilent 1100 Reference description 5 9 Column
19. PC it is the recommended setting e For the LAN card in the PC use TCP IP settings with a fixed IP address for example Clarity Agilent 1100 Note Note Installation Procedure IP address 192 168 254 12 Subnet mask 255 255 255 0 the last IP address section should be different from the 1100 IP address When connected to a network the address above should be assigned to the 1100 system contact your LAN administrator Stored Settings When this address could not be used due to network constraints it can be changed from the default settings by following procedure Use the Run command from the Windows Start menu Run the CMD EXE with following commands telnet 192 168 254 11 default or stored adress ce C WINDOWS system32 cmd exe iC2 gt telnet 192 168 254 11 IP 192 168 254 12 desired IP address Agilent Technologies TalkToLab 254 12 Dip 192 168 Quit ex C WWINDOWS system32 cmd exe quit ng changes succeeded Connection to host lost IC gt After this switch off the Agilent 1100 and change the dip switch settings on the LAN card to Using Stored SW5 ON and SW6 OFF The stored address can be also changed using the Handheld Controller G1323A B Bootp Settings The Chemstation usualy uses the bootp server to assign an IP adress to the Agilent 1100 instruments The DIP switches on the LAN card are SW5 OFF and SW6 ON or OFF in such case Set the appropriate
20. ailable communi cation options In older systems the LAN commu nication interface was optional and HP IB standard In recent new systems and in the 1200 series modules the HP IB port is no more present Note Cables are not part of Clarity Control Module It is strongly recommended to order required cables together with the Control Module Clarity Agilent 1100 Installation Procedure 3 Installation Procedure 3 1 Installing the GPIB interface in the PC Caution If you are using the 82357A USB GPIB Interface then leave out the first step and plug in the GPIB device after you have installed the drivers Insert the 82350A PCI GPIB Interface in the PCI slot e Install the corresponding drivers SICL and VISA provided by the interface manufacturer nin rie Geb Note The communication was tested on the GPIB driver Run to Config gt library version MO1 01 The IO libraries are also r torent i available on www agilent com find iolib sanm oe e Plug in the 82357A USB GPIB Interface Ext e In the right part of the Windows taskbar click About Agilent 10 Libraries Control s k the IO icon In the Agilent IO Libraries Configuration dialog select your GPIB card Agilent IO Libraries Configuration 10 Config Eel Eile Options Help 10 Config configures and edits Agilent I0 interfaces To configure a new interface select the interface in Available Interface Types and click Configure To edit a configu
21. ation of the FLD detector 41 Clarity Agilent 1100 5 6 5 6 1 Reference description Hardware Configuration Model Type Unknown SerialNo 01 Manufacturing Unknown Calibration Unknown Slit type Unknown Cancel Fig 29 Hardware Configuration Info MWD G1365 Agilent 1100 Configuration MWD Agilent 1100 Configuration Type G1365 Multi Wavelength Detector Serial number 01 Channels 4 Leen of signal Signal MWD Detector r Signal2 MWD Detector 2 r Signal3 MWD Detector 3 r Signal4 MWD Detector 4 Common MWD Cancel Help Fig 30 Agilent 1100 Configuration MWD Type Type of Agilent 1100 component indicated by name and code number Serial Number Serial number must correspond to the serial number imprinted on a label on the device Channels Number of independent data channels Signal n Sets the names of individual signals detectors 42 Clarity Agilent 1100 Reference description Inversion of Signal Inverts the polarity of the respective signal 5 6 2 Method Setup Acquisition Signal Method Setup Default1 Select Detector MWD Detector 1 x Enabled 4 MWD Detector 1 1100 MWD Method Signal MWD MWD Detector 4 MWD Detector IV Extemal Start Stop Range mV Sample nm Bw nm Ref nm 10000 z 100 m 360 Common Rate Hz 10 I Autozero before run T Autozero after un
22. d by name and code number Serial Number Serial number must correspond to the serial number imprinted on a label on the device Channels Number of independent data channels Signal n Sets the names of individual signals detectors Inversion of Signal Inverts the polarity of the respective signal 27 Clarity Agilent 1100 Reference description 5 4 2 Method Setup Acquisition Signal Method Setup Default1 Select Detector DAD Detector 1 v Enabled DAD Detector 1 DAD Detector 2 t 1100 DAD Method z DAD Detector 3 Signal DAD DAD Detector 4 DAD Detector IV Extemal Start Stop Range mV Sample rm Bw nm Ref nm Bw nm 100 x 4 f a a Common Rate Hz 10 J Autozero before run T Autozero after run Slit nm f4 Margin for neg absorb m4U I Run time min I Post time min Not Connected AS Control LC Control Measurement Acquisition Integration PDA Method Calculation Cancel Apply Report Fig 17 Method Setup Acquisition Signal Note Detector 1 External Start Stop Enables control through the use of an external event The digital detectors distinguish Start and Stop events so it is not necessary to specify the Start Start Start Restart etc options like with the A D converters Range mV Sets the signal range of the detector Sample nm Sets the centre of t
23. d in the device Clarity Sampler A Sampler Agilent 1100 Sampler cannot read method from file 1 Konkurence KNAUER Simulace S1000 test MET The method file was probably destined for other controlled instrument If you want to preserve the original method file use the Save As command in the Instrument window Fig 5 Cannot read method message box This message is normal when the method is first time used with new or modified configuration of the controlled instruments The message requesting confirmation of sending changed method to Agilent 1100 instrument will appear under default settings Agilent 1100 Method DEFAULT2 x The method has been changed send to Instrument No Help current action as default and perform it without mpt in the future Fig 6 Changed method dialog Click the No button If you have some methods already stored in the Agilent 1100 device it is possible to download them to Clarity Open the respective tabs of the Method Setup dialog AS Control Acquisition Thermostat and download the methods using the From device name button It is currently not possible to download the LC method from the device to Clarity 15 Clarity Agilent 1100 Using the Control Module 4 Using the Control Module Depending on the selected component appropriate new tabs will appear in the Method Setup dialog These are used for setting the corresponding parts of the Agilent 1100 in
24. divided by 100 Flow ml min Indicates the overall flowrate through the column The entered value applies to the time specified in the corresponding row 55 Clarity Agilent 1100 Caution Reference description If the flowrate set for the given pump in the Gradient Table calculated from percentage and total flow will exceed the maximum flowrate for the set pump head the change will not be accepted and may invoke a communication error Parameters Standby Flow Indicates the overall flowrate through the column in the STANDBY state reached after the last row of the table has been performed and the Time to Standby has passed The time period during which the flowrate is so maintained is defined by item Standby Time The ratio of individual components in the respective STANDBY and IDLE states is given by the first row of the Table the Initial row Time to Standby min Indicates the time during which the flowrate varies continuously between the last values entered in the table and the value defined by Standby Flow This time is included in the analysis time the CONTROL state Standby Time min The time during which the flowrate is maintained at Standby Flow This time is included in the analysis time CONTROL state Idle State An item specifying the overall flowrate through the column outside the instrument method The following states are possible Pump Off The flowrates of all components are zero
25. e Communication protocol GPIB C TCP Add Remove Note Default factory settings are listed in chapter 6 3 Recommended GPIB addresses on pg 62 Caution The interface address set for the GPIB interface in Agilent IO Libraries configuration IO Config window should be different from those used for the connected Agilent 1100 components Skip to the Common procedure LAN communication Agilent 1100 Configuration poured C GPIB Auto Detect GPIB Board Address 26 IP Address 192 168 254 11 Port 9100 Add Remove bes Cancel Help e Set the IP Address of the Agilent 1100 system e Set the Port address used 9100 by default Note The Port address in the Agilent 1100 system can be changed using the Handheld Controller G1323A B 12 Clarity Agilent 1100 Installation Procedure Common procedure for both GPIB and LAN Note Note e Use the Auto Detect button Clarity will determine the Agilent 1100 configuration automatically Tabs of individual components of the Agilent 1100 configuration will appear Go to the individual tabs and set the desired parameters for each component e g names of detectors etc Skip directly to the 3 3 1 Assign the control to Clarity Instrument Manual Configuration Manual configuration can be performed if the automatic configuration did not detect all components correctly or if you want to customize the configu
26. e can be viewed in any text editor Reset On will erase the log each time the station is restarted In case the record is needed it must be renamed copied or moved before the Clarity is started again Off the log will not be reset it can increase substantially after some time The typical example settings may be for GP IB GPIBO 26 Echo On 66 Clarity Agilent 1100 Note Troubleshooting textmode on filename CommDrv_1100 log reset on or for LAN communication 192 168 254 11 9100 Echo On textmode on filename CommDrv_1100 log reset on This record is very helpful for troubleshooting the communication between Clarity and the device 67
27. e if the respective lamp is switched off 5 4 4 Method Setup Acquisition Outputs Method Setup PDA_test Select Detector DAD Detector 1 X M Enabled Agilent 1100 DAD Method Signal DAD Outputs Time Table Analog output 1 Zero offset 5 1 00 Open Close Open Ciose eii 1000 10 00 Close Open Close E Range Y Vv X Analog output 2 Zero offset Attenuation mAU Range V Not Connected Measurement Acquisition Integration PDA Method Calculation Cancel Apply Report Fig 19 Method Setup Acquisition Signal 31 Clarity Agilent 1100 Reference description Analog Output x Zero Offset Sets the zero offset voltage for Analog Output 1 2 to a percentage of the full scale voltage Attenuation mAU Selects analog output attenuation The DAD offers two analog outputs signal A at analog output 1 and signal B at analog output The attenuation can be changed in power of 2 steps between 2000 mAU and 0 98 mAU Range V Sets the output signal Range to 1 V or 0 1 V Table The Output relays on detector are optional feature of certain Agilent 1100 configuration If it is present in your configuration then the table will enable to set the timetable of events to trigger these outputs 5 4 5 Method Setup Acquisition Time Table Method Setup PDA_test Select Detector DAD Detector 1 Enabled Agilen
28. elect Detector DAD Detector 1 Enabled Agilent 1100 DAD Method Signal DAD Outputs Time Table Spectrum Store Range mv 10000 E From nm J 130 To nm 400 Step nm 2 Lamps set state after send method F UV lamp I Vis lamp check state before run F UV lamp I Vis lamp Not Connected AS Control LC Control Measurement Acquistion Integration PDA Method Calculation Cancel Apply Report Fig 18 Method Setup Acquisition DAD Spectrum Store Spectral data will be acquired and stored in the chromatogram Every 224 Spectrum Leaves out every second spectrum to reduce the data throughput Range mV Sets the signal range for spectra From xxx To xx Step xxx Sets the wavelength range and stepwidth for spectra The stepwidth defines the distance in nm between two adjacent data points Valid Values 30 Clarity Agilent 1100 Reference description From 190 950 lower wavelength limit nm To 190 950 upper wavelength limit nm Step 1 100 stepwidth nm Lamps Set state after send method Checkboxes UV Lamp Vis Lamp will set the respective lamp ON after sending the method in case it is OFF Check state before run Configure if UV light source UV Lamp or visible light source Vis Lamp is required Determines whether a method requires the selected lamp or not The module becomes NOT READY stat
29. ence description 5 7 2 Method Setup Acquisition Signal Method Setup Default1 Agilent 1100 VWD Method Signal YwD Outputs Time Table Detector 1 IV Extemal Start Stop Range mV Sample nm p Polarity 10000 gt Dis Me Common Rate Hz 687 Autozero before run I Autozero after run Margin for neg absorb m4U I Run time min T Post time min Not Connected AS Control Lc Control Measurement Acquisition Integration PDA Method Calculation Cancel Apply Report Fig 36 Method Setup Acquisition Signal Detector 1 External Start Stop Enables control through the use of an external event Note The digital detectors distinguish Start and Stop events so it is not necessary to specify the Start Start Start Restart etc options like with the A D converters Range mV Sets the signal range of the detector Sample nm Sets the signal wavelength of the detector Polarity Sets the polarity of data handling for the detector For positive polarity the detector output positive data if detecting higher absorbance compared to the value during balance With negative polarity the detector output positive data if detecting lower 49 Clarity Agilent 1100 Reference description absorbance compared to the value during balance Common Rate Hz Selects the data acquisition rate for the stored signals
30. ended GPIB addresses on pg 62 Problem 3 Aborting Activity Occasionally some Agilent 1100 module may get to the ABORTING ACTIVITY state referred in the status line of the Method Setup control tabs The red diode is indicating Error status on the module Usually it is necessary to e Close Clarity Software e Switch off all the Agilent 1100 modules e Wait for few seconds e Switch on the Agilent 1100 modules e Start Clarity after the modules have been initialized Problem 4 Not Connected Occasionally some or all Agilent 1100 modules may get to the NOT CONNECTED state referred in the status line of the Method Setup xxControl tabs This may be caused by some error in the communication Usually it is necessary to 63 Clarity Agilent 1100 Troubleshooting Close the Clarity Software Switch off all the Agilent 1100 modules Wait for few seconds Switch on the Agilent 1100 modules Start Clarity after the modules have been initialized Problem 5 Not ready problem When autosampler is not injecting some module may be NOT READY Yellow diode is lighted on this module Check that the required lamps in detectors and the thermostat has been turned ON When the autosampler detects even temporary NOT READY state of some module it will wait for 1 minute and then it will retry the injection Problem 6 Analysis stop problem When LC pump is configured on the system the Analysis time is
31. etector MWD Detector 1 Enabled Agilent 1100 MWD Method Signal MWD Outputs Time Table Signal change Not Connected Measurement Acquisition Integration PDA Method Calculation Cancel Apply Report Fig 34 Method Setup Acquisition Time Table Time min Sets a time for the desired change Zero time is not permitted value Prep Autozero will be performed when checked Signal Change Select the signal 1 4 to set a new wavelength Sample nm Set the sample wavelength Bw nm Set the Sample bandwidth Use ref Reference wavelength will be used when checked 47 Clarity Agilent 1100 Reference description Ref nm Set the reference wavelength Bw nm Set the Reference bandwidth 5 7 VWD G1314 5 7 1 Agilent 1100 Configuration VWD Agilent 1100 Configuration Type G1314 Variable Wavelength Detector Serial number 01 Inversion of signal Signal YWD Detector 1 r Common ywD Cancel Help Fig 35 Agilent 1100 Configuration VWD Type Type of Agilent 1100 component indicated by name and code number Serial Number Serial number must correspond to the serial number imprinted on a label on the device Channels Number of independent data channels Signal n Sets the names of individual signals detectors Inversion of Signal Inverts the polarity of the respective signal 48 Clarity Agilent 1100 Refer
32. etector 3 FLD Parameterd FLD lt Detector 4 ble Acquisition Mode Base Wavelength Multi wavelegth Mode Fluorescence Excitation Detector 1 Excitation Phosphotescence C Zero Order remem Chemoluminiscence e 230 nm Spectra Acquisition E Delay ig C Zero Order on p A From Gate 1 e 460 nm pann To IV Extemal Start Stop p Phosphorescence Emission T Run time min PMT Gain 2 I Post time min Rate H2 Not Connected AS Control LC Control Measurement Acquisition Integration PDA Method Calculation Cancel Apply Report Method Setup Acquisition FLD Parameters Acquisition Mode Selects among Fluorescence Phosphorescens or Chemoluminiscence acquisition modes 35 Clarity Agilent 1100 Caution Note Reference description Phosphorescence Sets the parameters of the Phosphorescence acquisition mode Delay us Determines the time between flash and Start_of_Integration Gate us Determines the time between Start of Integration and End of Integration If flash frequency is 370 Hz the Economy Mode on the Flashlamp tab is checked and Delay Gate fields sum is greater than 4 5 msec then the flash frequency will automatically be reduced to 74 Hz External Start Stop Enables control through the use of an external event The digital detectors distinguish Start and Stop events so it is not necessary
33. etup Acquisition Depending on the configured detectors new tabs will appear in the Method Setup Acquisition dialog The individual variants of the Acquisition dialogs are described in the corresponding sections of chapters 5 4 5 7 4 4 Method Setup Thermostat The Thermostat tab is available only when the Column Compartment is installed See the chapter 5 9 Column Compartment G1316 on pg 58 4 5 Data Acquisition The Data Acquisiton window displays online signal from the detectors It also provides access to analysis controlling commands such as Start Stop etc For detailed description of the Data Acquisition window see the Clarity Reference Guide Agilent 1100 Data Acquisition File Analysis Display view Window Help QU Ai lt amp mi Tine range 1 min Voltage range 10 mV Always on Top v Toolbars Customize Reset All DAD Detector 1 DAD Detector 2 Fixed Axes DAD Detector 3 Floating Axes DAD Detector 4 Context Axes Grid Lines T 02 Voltage mY Fig 7 Data Acquisiton The graph can also display acquired spectra using the WA View Show Spectrum 17 Clarity Agilent 1100 Using the Control Module command active only during running acquisition Customize Toobar Command Categories Buttons Menu CELTI Window Select a category then click a button to see its description Drag the button to any toolbar Descript
34. governed by the length of the gradient defined by the last line in the gradient table The table should always contain at least two lines even when running isocratically When only one line is set and the Run time is not specified in other modules the Agilent 1100 may be in RUNNIG STATE green diodes on even after the analysis time in Clarity has elapsed The system can be in such case stopped by the Stop command icon from the LC Monitor window The initial conditions will be resumed after using the Resume Initial conditions command or sending the method Problem 7 Agilent 1100 Module Error Occasionally some Agilent 1100 module may get to the ERROR state referred in the status line of the Method Setup xxControl tabs The red diode is indicating Error status on the module A message box describing the error may appear in Clarity 64 Clarity Agilent 1100 Troubleshooting Most common causes will be missing vial in the autosampler exceeded pressure limits on the pump or leak detected by the solvent sensors on some module First it is necessary to remedy the cause after sending the method from Clarity the operation can be usually resumed Otherwise it may be necessary to restart the system Problem 8 Clarity stays in the Waiting state after Agilent 1100 stopped the analysis When the analysis is stopped from the device equipped with autosampler unkown reason of the stop Clarity waits two minutes and
35. he wavelength of the detector Bw nm Sets the bandwidth for the sample wavelength in nm Valid Values 2 400 28 Clarity Agilent 1100 Reference description Use reference unlabeled checkbox Use the reference wavelength to compensate for lamp fluctuations Ref nm Sets the center of the reference wavelength in nm Valid Values 0 190 950 Bw nm Sets the bandwidth for the reference wavelength in nm must be O if reference is switched off 190 lt Ref Bw 2 lt 950 Valid Values 0 2 400 Common Rate Hz Selects the data acquisition rate for the stored signals Autozero Before Run Resets the detector to zero at the beginning of the analysis Autozero After Run Resets the detector to zero after finishing the analysis Slit nm Defines the width of the built in microslit in its open position The instrument performs a zero automatically whenever the Slit parameter has been changed Margin for neg absorb mAU Maximal negative value of absorbance that will be still taken into account Run Time min Run time of the analysis Data acquisition will be finished after the specified time unless a shorter time is specified in another module 29 Clarity Agilent 1100 Reference description Post Time min The system will stay in running state for the specified time after the analysis is finished 5 4 3 Method Setup Acquisition DAD Method Setup Default1 S
36. in micro litres per second This parameter determines how fast the metering device moves to draw up sample 23 Clarity Agilent 1100 Reference description Eject Speed ul min Sets speed for ejecting sample in micro litres per second This parameter determines how fast the metering device moves to eject sample Draw Position Offset mm Needle draw eject position offset Defines the offset of the needle from the default draw position in a vial O means default draw position negative values move needle closer to the bottom of the vial 5 3 3 Method Setup AS Control Program The Program tab is used to display and modify injection program of the autosampler Method Setup Default1 HP1100 Autosampler Method Injection Program Amount Speed Offset Instruction ul ul min Source Vial mm Draw Def Det Sampe Def v Eject a Mix le Inject A Wait Needle AS Status Description Agilent 1100 AS Status Not Connected AS Control Measurement Acquisition Integration Calculation Cancel Apply Report Fig 14 Method Setup AS Control Program Working with the Injection program is simple Compose the command in the line above the table Use the Insert button to add it to the table and the Change and Delete buttons to modify the program table Instruction Displays a list of available operations 24
37. ion Cancel Help Fig 8 Customize It is also possible to add VA Show Spectrum icon to the toolbar for fast setting of the spectral mode as well as E Set Zero and Reset Zero To do this use the View Customize command The Show Spectrum icon can be found on the Customize Commands dialog in the Analysis set of commands 18 Clarity Agilent 1100 Using the Control Module 4 6 Device Monitor The pump status window can be invoked using the Monitor Device Monitor command from the Instrument window or using the Device Monitor icon from the Instrument window Agilent 1100 Device Monitor ste Control view Window Help P ii EA LC Monitor FA Total Flow mL min 0 000 Pressure MPa E Ed Agilent 1100 TAS Ei Agilent 1100 DAD Ed Agilent 1100 COLCOMP For Help press F1 Fig 9 Device Monitor This window provides actual condition readings from the pumps The pump can be stopped from this dialog by using the Control Stop command or by the icon This will stop the running data acquisition as well as the pump flow The flow will be resumed by using the Apply or OK buttons in the Method Setup dialog The individual solvent flow can be displayed either in flow units or as a percentage of total flow by checking the View Component flow in command 19 Clarity Agilent 1100 Reference description 5 Reference description Note This chapter provides
38. isition Outputs Method Setup DEFAULT2 Agilent 1100 VWD Method Signal vwD Outputs Time Table 1 00 Open Close pen Not Connected Measurement Acquisition Integration PDA Method Calculation Cancel Apply Report Help Fig 38 Method Setup Acquisition Outputs Time min Sets a time for the desired change Zero time is not permitted value Cont no 1 4 Sets the desired state of the Output to be set at the specified time 52 Clarity Agilent 1100 Reference description 5 7 5 Method Setup Acquisition Time Table Method Setup DEFAULT2 Agilent 1100 VD Method Signal VWD Outputs Time Table Sample Inm 220 Not Connected Measurement Acquisition Integration PDA Method Calculation Cancel Apply Report Fig 39 Method Setup Acquisition Time Table Time min Sets a time for the desired change Zero time is not permitted value Prep Autozero will be performed when checked Signal Change Select the signal 1 4 to set a new wavelength Sample nm Set the sample wavelength 5 8 LC Pump G1310 G1311 G1312 Setting of the LC pumps Following pumps are available Isocratic G1310 Binary G1311 Quaternary G1312 See the chapter 4 4 Method Setup Thermostat on pg 17 53 Clarity Agilent 1100 Reference description The Method Setup LC Control dialog sets the pa
39. l be switched on during Agilent 1100 startup or not The button enables to turn the lamp ON or OFF manually 5 4 7 Hardware Configuration Info Displays the configuration of the DAD detector Hardware Configuration Model Type Unknown LICOP Unknown Serial No 26 Manufacturing Unknown Calibration Unknown Slit type Unknown Cancel Fig 22 Hardware Configuration Info 5 5 FLD G1321 5 5 1 Agilent 1100 Configuration FLD Agilent 1100 Configuration Type G1321 Fluorescence Detector Serial number 01 Channels 4 4 Inversion of signal Signal 1 FLD Detector r Signal 2 FLD Detector 2 r Signal 3 FLD Detector 3 a r Signal 4 FLD Detector 4 Common FLD Cancel Help Fig 23 Agilent 1100 Configuration FLD 34 Clarity Agilent 1100 Reference description Type Type of Agilent 1100 component indicated by name and code number Serial Number Serial number must correspond to the serial number imprinted on a label on the device Channels Number of independent data channels Signal n Sets the names of individual signals detectors Inversion of Signal Inverts the polarity of the respective signal 5 5 2 Method Setup Acquisition FLD Parameters Sets the basic parameters of the FLD detector Method Setup Default1 Select Detector FLD Detector 1_2 x M Enabled g FLD Detector 1 FLD Detector 2 enit 1100 FLD Method FLD D
40. n will be performed without wash or program Injection with Needle Wash Before each injection the needle will be washed in the vial specified in the Wash Vial field Wash Vial Sets the number of the vial to be used for needle washing in the Injection with Needle Wash mode 22 Clarity Agilent 1100 Reference description Injection Program Applies injection program specified in the Program tab Method Setup pda1 Common for all detectors HP1100 Autosampler Method Injection Program From AS Standard Injection Injection with Needle Wash Wash Vial C Injection Program Prog ID Run Time min Post Time min Draw Speed ul min Eject Speed pl min Reset Draw Position Offset mm AS Status Description Agilent 1100 AS Status Not Connected AS Control LC Control Measurement Acquisition Thermostat 1 Integration PDA Method Calculation Cancel Apply Repot Fig 13 Method Setup AS Control Injection Click Edit to switch to the Program tab From AS will load injection program with the specified Prog ID from the autosampler Run Time min Run time of the analysis Data acquisition will be finished after the specified time unless a shorter time is specified in another module Post Time min The system will stay in running state for the specified time after the analysis is finished Draw Speed ul min Sets speed for drawing sample
41. nt 1100 G1329 Tray Configuration Connection Board gpib0 Address 26 100 x 1 8 ml Syringe Volume yi ul Thermostat OFF Switch ON Seat Capillary 900 0 vj u I Switch Thermostat on at Startup I Not Ready Until Temperature Reached Temperature OFF ca AS Apply Cancel Fig 15 Hardware Configuration In the top section the Type of Sampler Connection and Tray Configuration are displayed Syringe Volume Selects the volume of the syringe in ul Seat Capillary Selects the volume of the seat capillary in ul Following items are displayed only for the thermostated sampler TAS Thermostat Displays the actual status the button allows for manual switching it ON OFF Switch thermostat on at Startup The thermostat will be set ON during the initialization Not Ready until Temperature Reached The autosampler will be Not Ready until the set temperature 2 C is reached 26 Clarity Agilent 1100 Reference description Temperature Setting of the desired temperature 5 4 DAD G1315 5 4 1 Agilent 1100 Configuration DAD Agilent 1100 Configuration Type G1315 Diode Array Detector Serial number 01 Channels 4 Inversion of signal Signal DAD Detector r Signal2 DAD Detector 2 r Signal3 DAD Detector 3 r Signal4 DAD Detector 4 Common DAD Cancel Help Fig 16 Agilent 1100 Configuration DAD Type Type of Agilent 1100 component indicate
42. ommidrv log utility cece eee e ence eee eeneeee 65 Clarity Agilent 1100 Agilent 1100 Control Module 1 Agilent 1100 Control Module The Agilent 1100 driver can control Agilent 1100 series and 1200 series in 1100 emulation mode HPLC systems with e DAD MWD VWD FLD and RID detectors e Quarternary Binary and Isocratic Pumps e Column compartments e Autosamplers standard and thermostated Fig 1 Agilent 1100 The direct control can be performed via e 82357A USB GPIB Interface e 82350A PCI GPIB Interface e LAN Note The 82355 and 82431 ISA GPIB Interfaces are not supported Data Acquisition can be performed via e GPIB Clarity Digital Data Acquisition e LAN Clarity Digital Data Acquisition e ANALOG analog signal to A D converter e g INT7 or U PAD Clarity Agilent 1100 Requierements 2 Requierements e Clarity Installation CD ROM with LC Control module p n A24 e AS control module p n A26 when the autosampler is used e PDA Extension p n A29 when the DAD or FLD detector is used e Cross LAN cable p n SK08 and the LAN card in the PC for the newer Agilent 1100 and all Agilent 1200 systems For older Agilent 1100 systems GPIB interface may be necessary This consists of e either 82357A PCI GPIB interface card IAG11 GPIB cable p n GPIB and free PCI slot in the PC e or 82350A USB GPIB interface card and free USB port in the PC Caution Check your Agilent 1100 for av
43. rameters of the LC pumps method gradient Method Setup PDA_test MODIFIED Gradient Table A Flow Imamin Standby Flow mL min 1 7 000 10 000 Time to Standby min 10 000 o 1 000 Standby Time min Idle State C Pump Off Initial Standby Options S Control LC Control Measurement Acquisition Thermostat 1 J Integration PDA Method Calculation Cancel Apply Report Help Fig 40 Method Setup LC Control Caution When pump is configured on the system the data acquisition time is governed by the last entry in the Gradient Table Even when running isocraticaly you must define at least two lines the last with the desired analysis time Caution When stopping pump from the LC Monitor the pump may go to Error state Status LED on the pump is in red The sending of method using the Send Method button from the Single Analysis dialog or OK or Apply button from the Method Setup dialog will clear this state Graph The graph depicts the percentage of components as a function of time together with the overall flowrate Data are taken over from the Gradient Table Changes effected in this table are immediately reflected in the graph Assignment of colors to individual components is shown in the header The assignment is fixed and individual components are displayed in the graph from bottom to top 54 Clarity Agilen
44. ration settings Use the Add button to invoke the Add Module dialog and add the modules manually Add module Module type Serial number 1 09 61314 Agilent 1100 Vw G1321 Agilent 1100 FLD G1315 Agilent 1100 DAD G1310 Agilent 1100 Isoctatic pump bncel G1311 Agilent 1100 Quart Pump G1312 Agilent 1100 Binary Pump 61313 Agilent 1100 AS 61329 Agilent 1100 TAS G1316 Agilent 1100 COLCOMP e Select the desired module from the Module Type listbox and set the Serial Number e Click OK A corresponding tab will appear in the Agilent 1100 Configuration dialog Repeat the above three steps to add all desired components e Go to the individual tabs and set the desired parameters for each component e g names of detectors etc e Press the OK button 13 Clarity Agilent 1100 Installation Procedure 3 3 1 Assign the control to Clarity Instrument e Switch to the desired Clarity Instrument x tab in the right part of the System Configuration dialog e Drag and drop the Agilent 1100 from the Setup Control Modules in the left to the instrument on the right or use the gt button in the centre System Configuration Setup Control Modules Number of Instruments Ci Instrument gt An Instrument 2 3 C Detector 6c Instrument Type Cee ei ceac i RE asic 1100 GC LC C EA C GPC Name Agilent 1100 Aa VWD Detector 1 Image for Closed Instrument Image for Opened Ins
45. red interface select the interface in Configured Interfaces and click Edit To automatically configure the interfaces identified with click Auto Config Available Interface Types Configured Interfaces VISA Type Interface Description VISA Name SICL Name ASRL RS 232 COM Ports ASRLI COM1 Auto Config ASRL VISA LAN Client e g 5810 GPIB 82350 PCI GPIB Card GPIB 82341 ISA GPIB Card GPIB 82357 USB to GPIB GPIB VISA LAN Client e g 5810 GPIB VXI_ GPIB VXI Command Module TCPIP LAN Client LAN Instruments USB Instruments VISA LAN Client for USB E8491 IEEE 1394 to VXI LAN Server PC as Server Lenore Edit Remove NOTE Auto Config will configure interfaces identified with an e Press Edit to invoke the Card Configuration dialog Clarity Agilent 1100 Note Caution Installation Procedure 82350 PCI GPIB Card Configuration Questions Press the Help button below Recommended default values are shown Serial Number T515 SICL Interface Name PE for backwards compatibility use hpib7 VISA Interface Name GPIBO Logical Unit 7 fa Help Cancel Bus Address 0a H Defaults I System Controller Edit VISA Config Fig 2 87350 PCI GPIB Card Configuration e Set the SICL Interface Name The BUS Address must be different from the address of any of the other components in the Agilent 1100 configuration recommended values are 21 or 30 21 is
46. strument device method AS Control Le Control Measurement Acquisition Thermostat 1 Integration PDA Method Calculation The Method Setup tabs contain The From button e g From AS From DAD etc that loads the instrument method from the corresponding device to the template method that is currently opened in the Instrument window The To button e g To AS To DAD etc that sends the sampler part of the template method opened in the Instrument window and displayed in the individual tabs of Method Setup dialog from Clarity to the corresponding device The Status opens the Hardware Configuration dialog listing the available hardware features of current configuration and enabling manual control of selected functions 4 1 Method Setup AS Control The Method Setup AS Control dialog sets the parameters of the sampler method Detailed description of the AS Control dialog can be found in chapter 5 3 Autosampler AS G1313 and TAS G1329 on pg 22 4 2 Method Setup LC Control Depending on the configured LC Pumps the Method Setup LC Control tab will appear in the Method Setup dialog This tab sets the parameters of the LC pumps method gradient Following pumps are available for Agilent 1100 Isocratic G1310 Binary G1311 Quaternary G1312 16 Clarity Agilent 1100 Using the Control Module See the chapter 5 8 LC Pump G1310 G1311 G1312 on pg 53 4 3 Method S
47. t 1100 Reference description The flowrate is displayed in black The graph has two vertical axes the axis on the left refers to the mixing ratio that on the right to the overall flowrate Gradient Table A table for setting the composition of the mobile phase and the overall flowrate as a function of time Operation is analogous to that of spreadsheets Excel Quatro Pro Upon clicking a cell by the left mouse button that cell is highlighted by dots and ready to receive values A cell that fails to highlight is not available for editing Time min The entered value represents the time at which the ratio of flowrates and the overall flowrate correspond to the values entered in the corresponding row These values vary continuously from one time to the next in a manner ensuring that the conditions specified in the next row are satisfied XXX1 4 Yo Represents the percentage of a component The designation XXX1 4 is in fact replaced by the name of the component items Solvent 1 4 in the Gradient Options Dialog box Should you enter a component value such that the sum of all values exceeds 100 the percentage in the last column is automatically adjusted if the percentage of the last compound is already zero the value of the currently entered component is adjusted instead The flowrate of a compound is calculated by multiplying the overall flowrate indicated in the Flow column by the corresponding percentage
48. t 1100 DAD Method Signal DAD Outputs Time Table Signal Sample Bw Use Bw change nm inm f ret nm 10 X 2 0 0 0 0 10 00 fq 3 272 350 amp C9 0 mm E Not Connected Measurement Acquisition Integration PDA Method Calculation Cancel Apply Report Fig 20 Method Setup Acquisition Signal 32 Clarity Agilent 1100 Reference description Time min Sets a time for the desired change Zero time is not permitted value Prep Autozero will be performed when checked Signal Change Select the signal 1 4 to set a new wavelength Sample nm Set the sample wavelength Bw nm Set the Sample bandwidth Use ref Reference wavelength will be used when checked Ref nm Set the reference wavelength Bw nm Set the Reference bandwidth 5 4 6 Hardware Configuration Main Hardware Configuration Main Info Model Type Agilent 1100 DAD Connect Demo UV Deuterium lamp On Vis Tungsten lamp Switched on at startup On Cancel Fig 21 Hardware Configuration Main UV Deuterium lamp Deuterium lamp configuration determines whether the Deuterium lamp will be switched on during Agilent 1100 startup or not The 33 Clarity Agilent 1100 Reference description button enables to turn the lamp ON or OFF manually VIS Tungsten lamp Tungsten lamp configuration determines whether the Tungsten lamp wil
49. then tries to re run the sequence this is repeated five times and then the analysis is stopped in Clarity Commadrv log utility When the cause could not be discovered easily the recording of communication between Clarity and the 1100 modules can significantly help the DataApex support to discover the cause It is possible to record the communication between Clarity and the Agilent 1100 system The recording can be enabled by amending the file COMMDRV IN in the Clarity installation directory The file can be edited in any text editor e g Notepad COM 1 Echo Off textmode on filename CommDrv1 log reset off 65 Clarity Agilent 1100 Note Troubleshooting For GP IB communication replace the port specification COM1 by the GP IB board SICL name and address as used in the Agilent 1100 Configuration for example GPIBO 26 For LAN comunication replace the port specification COM1 by the device IP address and Port as used in the Agilent 1100 configuration for example 192 168 254 11 9100 Separate entries can be specified for each Com port or device Folloving parameters should be set also Echo On communication will be recorded Off default will not record any communication Textmode Filename The file where the communication record should be stored If the path is not specified the file will be stored in the same folder like the COMMDRV INI file The created log fil
50. ti wavelength Mode If the On checkbox is not checked the spectra will not be acquired If it is On the From To and Step fields can be filled in to specify the spectrum to be acquired A Spectral Detector must be configured on the Clarity Instrument see pg 14 example otherwise this section will be disabled 37 Clarity Agilent 1100 Reference description 5 5 3 Method Setup Acquisition Flashlamp Method Setup PDA_test Agilent 1100 FLD Method FLD Parameters Flashlamp Outputs Time Table I Check Flash Lamp Lamp on Always Only During Analysis I Economy Mode M Lamp Energy Reference AS Control LC Control Measurement Acquisition Thermostat 1 Integration PDA Method Calculation Cancel Apply Report Fig 25 Method Setup Acquisition Flashlamp Check Flash Lamp Determines whether a method requires the flashlamp or not The module will get in NOT READY state if the flashlamp is switched off while this parameter is checked Lamp On Sets the flashlamp off or on in different modes Always Lamp will be always on Only During Analysis Lamp will be ON only during analysis Caution The Lamp must be switched ON after the detector is switched on if the Only During Analysis option is active 38 Clarity Agilent 1100 Reference description Economy Mode Sets the Flashlamp Economy mode Multi wavelength mode standard mode flash frequency
51. tional feature of certain Agilent 1100 configuration If it is present in your configuration then the table will enable to set the timetable of events to trigger these outputs 5 5 5 Method Setup Acquisition Time Table Method Setup PDA_test Agilent 1100 FLD Method FLD Parameters Flashlamp Outputs Time Table Time min 1 00 0 1 2 5 00 200 E 3 Emission Ex Not Connected AS Control LC Control Measurement Acquisition Thermostat 1 Integration PDA Method Calculation Cancel Apply Report Fig 27 Method Setup Acquisition Time Table 40 Clarity Agilent 1100 Reference description Time Sets a time for the desired change Zero time is not permitted value Emission Excitation Sets the Emission Excitation wavelength according to the multiwavelength mode selected Ex n Em n Sets the respective wavelengths according to the multiwavelength mode selected PMT Gain Sets the PMT Gain 5 5 6 Hardware Configuration Main Hardware Configuration Jino Model Type Agilent 1100 FLD Connect Demo Flashlamp I Switched on at startup On Cancel _ Fig 28 Hardware Configuration Main Flash lamp Determines whether the flash lamp will be automatically switched on during Agilent 1100 startup or not The button enables to set the lamp ON or OFF manually 5 5 7 Hardware Configuration Info Displays the configur
52. trument An FLD Detector 1 An FLD Detector 2 An FLD Detector 3 Aa FLD Detector 4 Aa FLD Spectral Detector An DAD Detector 1 AA DAD Detector 2 AA DAD Detector 3 L Aa DAD Detector 4 C Detector Aa DAD Spectral Detector Aa FLD Detector 1 Agilent 1100 te RA Quart Pump HP1100LC 1 g Isocratic pump HP1100LC1 Agilent 1100 9 Quart Pump HP1100LC 2 as KI Quart Pump HP1100LC 3 SAS Sampler 1 Agilent 1100 Quart Pump HP1100LC 4 2 PDA Q Binary Pump HP1100LC 1 Ae FLD Spectral Detector jlent 1100 Binary Pump HP1100LC 2 AS Sampler 1 Instrument 1 SA TAS Sampler 1 Aniston Card O COLCOMP Thermostat Instrument 1 Start Dig Input Agilent 1100 FLD Ready Dig Output Agilent 1100 FLD rf Remove About Setup Fig 4 System Configuration Caution Even if signals from DAD and FLD detectors can be acquired simultaneously up to maximum of four on one instrument only one spectral detector can be configured Caution The spectral detector can be configured only if there is at least one detector signal from the same device configured on the Clarity Instrument see the example 3 4 First start of Clarity after configuration At the first start Clarity may display a warning message that the stored Agilent 1100 14 Clarity Agilent 1100 Note Installation Procedure instrument method stored in Clarity does not match with the metho
53. ture control Off or On Temp C Sets the right temperature value Right Temp Switches the Right temperature control Off or On Temp C Sets the left temperature value Column Switches the column selection valve position 60 Clarity Agilent 1100 Connections 6 Connections 6 1 Wiring Caution In case of complete system with autosampler pump and detector only the HP IB communication line is needed When using a system without autosampler or single modules detectors pumps only they need to be started using the Remote external event contact connector The connector is 9 pin Sub D Receptacle Canon female on the backside of the module marked Remote Use the Pin 1 to Pin 3 Start contacts to connect the injector The pins 1 to 8 may be used to Stop the run alternatively Next to it is another connector of the same type marked RS232 6 2 Description of connectors 6 2 1 LAN CAN only Caution inter module communication LAN interface The LAN interface board is installed usualy in only one component of the Agilent 1100 system On others a cover plate is in its place ication only F Be careful that you connect the LAN cable to the LAN Interface and NOT to one of the CAN connections The CAN bus uses 12 Volt signals and a misconnection to the CAN bus may destroy network equipment on the other end of the cable 61 Clarity Agilent 1100
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