Manual User - mecalab.ca
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1. Germany Tel 49 6441 210 010 Fax 49 6441 210 0122 Motic Spain S L Spain Pol gon Industrial Les Corts Cam del Mig 112 08349 Cabrera de Mar Barcelona Spain Tel 34 93 756 6286 Fax 34 93 756 6287 Website http www motic com E mail digital info motic com Motic China Group Ltd China Motic Building Torch Hi Tech Industrial Development Zone Xiamen P R C Tel 86 0592 562 7866 Fax 86 0592 562 7855 2007 2010 Motic China Group Co Ltd All rights reserved Motic is a registered trademark and service mark of Motic China Group Co Ltd Microsoft Windows logo is a registered trademark of Microsoft Corporation All other trademarks are the property of their respective owners Design Change The manufacturer reserves the right to make changes in instrument design in accordance with scientific and mechanical progress without notice and without obligation Updated 2011 012. Set the Phase slider to the centre position in standard configuration this position does not contain an illumination annulus Fig 24 In case all 3 positions of the slider are occupied due to personal setup of the microscope pull out the complete slider Bring the specimen into focus The aperture diaphragm of the condenser is needed for adjusting the numerical aperture N A of the illumination Closing the aperture diaphragm will lower the resolution and brightness but increase the contrast and depth of focus An image with appropriate contrast in most cases can be obtained with an aperture diaphragm closed down to 2 3 Fig 24 Phase Contrast Microscopy Phase contrast objectives are labelled Ph PhO Ph1 Ph2 For using phase contrast be sure to use the annular ring in the slider has the same phase label as the objective PhO Objective 4x Ph1 Objective 10x 20x 40x Ph2 Objectives 20x 40x Always open the aperture diaphragm completely If the aperture diaphragm is closed it will obstruct the annular diaphragm and the phase contrast effect cannot be obtained Bring the 10x Ph1 objective into optical path Position the Phase annular diaphragm slider to Ph1 19 Remove one eyepiece from the eyepiece tube and insert the phase centering telescope instead Fig 25 Loosen the fixing screw on the centering telescope By pulling out the inner part of the centering telescope you may focus on a dark ring inside the
3. a piece of gauze with diluted detergent and wipe clean e For plastic components only moisten a piece of gauze with water and wipe clean 3 When Not in Use e When not in use cover the instrument with vinyl dust cover and store in a place low in humidity where mould is not likely to form Store the objectives eyepieces and filters in a container or desiccator with drying agent Note If equipment is used in a manner not specified by the manufacturer the protection provided by the guarantee terms may be impaired 25 4 Warning Label The following warning symbols are found on the microscope Study the meaning of the warning symbols and always use the equipment in the safest possible manner Warning Label Symbol Explanation Indicates that the surface becomes hot and should not be touched with bare hands A CAUTION Risk of danger See user manual Proper handling of the microscope will ensure years of trouble free service If repair become necessary please contact your Motic agency or our Technical Service directly 26 4 Motic CE OI NO 1300901107651 Motic Incorporation Ltd Hong Kong Rm 2907 8 Windsor House 311 Gloucester Road Causeway Bay Hong Kong Tel 852 2837 0888 Fax 852 2882 2792 Motic Instruments Inc Canada 130 4611 Viking Way Richmond B C V6V 2K9 Canada Tel 1 877 977 4717 Fax 1 604 303 9043 Motic Deutschland GmbH Germany Christian Kremp Strasse 11 D 35578 Wetzlar
4. both eyes easier this adjustment also reduces the extent to which focusing is lost when the objective magnification is changed In particular this occurs when a low magnification objective is used Set the diopter on both eyepieces to the 0 position Swing in the 10x objective focus with one eye only as everybody has got a strong eye use this one for this first focussing Fig 18 Fig 19 When in focus close this eye and use the other one Focus by only using the diopter ring on the respective eyepiece do not use the coarse fine knob Change to a higher magnification and repeat the complete procedure As the focus depth is less in high magnification lenses a precise adjustment of the diopters here is easier Keep this final diopter position for all lenses 16 Coarse and fine focusing For focusing of the instrument please use the coarse and fine focus knobs on the left and right side of the microscope stand The direction of vertical movement of the revolving nosepiece corresponds to the turning direction of the focus knobs One full rotation of the fine focus knob moves the nosepiece 0 2mm in z direction One scale unit on the fine focus knob equals 2 microns Please avoid following action Never attempt either of the following actions since doing so will damage the focusing mechanism Rotate the left or right knob while holding the other Turning the coarse and fine focus knobs further than their
5. halogen bulb used as a light source has higher luminance and colour temperature than conventional tungsten lamps The luminance of halogen bulb is approximately four times brighter than the conventional tungsten lamps As long as the lamp voltage is kept constant the halogen lamp maintains the same level of brightness and colour temperature regardless of whether it is new or nearing the end of its life span 1 2 LED module The LED module is specially designed to be inserted into halogen bulb socket directly converting halogen illumination to LED illumination LED is more economical and environmental friendly and combines the advantages of low heat and long life span 2 Filter Holder y Fig 9 Fig 10 e Filter holder is located under the lamphouse Fig 9 for easy replacement of the filter Fig 10 12 3 Mounting the Condenser e Mount the condenser on the dovetail mount of the condenser holder Fig 11 with the aperture diaphragm lever facing the front Secure it with the clamp screw by the 2 5mm hexagonal screwdriver supplied with the microscope Fig 12 _ Fig 11 Fig 12 e f Phase contrast is to be used insert the Phase slider into the slot of the condenser The centering screws of Phase slider have to face the front side Fig 13 M 1 5mm Hexagonal screwdriver Oo EEE Fig 13 4 installing the Objectives e Remove the stage insert from the stage Fig 14 e Screw in the objective
6. limit Coarse focus torque adjustment To increase the torque turn the torque adjustment ring located behind the left hand coarse focus in the direction of the arrow To reduce the torque turn the ring in the direction opposite gt a Fig 20 1 Coarse Focus Torque Adjustment Ring 2 Coarse Focus 3 Fine Focus 17 5 Changing between Halogen and LED illumination Attention Unplug the plug in power unit from the microscope to avoid any risk of electric shock Before replacing the bulb changing to LED please wait for a sufficient cool down time of the bulb e Press down lightly the lamp house cover and rotate it counter clockwise for removal Fig 21 Remove the bulb from the socket pinholes sa Halogen bulb Fig 22 LED Fig 23 e Installing the new bulb into the socket pinholes until it reaches the limited be careful not to tilt it when mounting e If installing the halogen bulb do not touch the glass surface of the lamp with bare fingers Doing so will cause fingerprints grease etc to burn onto the lamp surface reducing the illumination provided by the bulb If surface is contaminated wipe it clean using lens or soft cotton e Return lamphouse cover to original position and rotate clockwise to lock into place The white paint marked on the cover should face the user e The halogen bulb holder can be used also for the LED module without modification 18 Brightfield Microscopy
7. phase contrast objective this circular phase plate is responsible for the phase effect Simultaneously you will realize the image of the illumination annulus which is projected into the same back focal plane by the condenser AD Fig 25 If the objective phase plate and the illumination annulus do not coincide use the two allen keys 1 5mm supplied with the microscope Fig 26 Insert the keys into the countersunk screws Turn the keys to move the illumination annulus in the slider until the image of the illumination annulus is concentric with the phase plate Fig 27 This complete procedure has to be performed for the respective phase lens illumination ring combinations If the image of the illumination ring is diverging from the phase plate in the objective a low phase contrast image will result For construction reasons the phase rings in the different objectives are located in different levels back focal planes So for each objective you will have to focus again with the centering telescope 20 Fig 26 Fig 27 To achieve maximum contrast in phase contrast a Green Interference filter is recommended It may be inserted in the filter mount of the condenser carrier To insert replace an annular diaphragm take care the diaphragm has got the correct orientation Fig 28 Push the diaphragm against the flexible filament until it fits in the hole Fig 29 If necessary use the allen keys to bring
8. the annulus to the center of the hole A fine adjustment will be done by using the centering telescope see above Fig 28 Fig 29 21 8 Filter selection Filter holder can hold two filters Filter type Procedure ND Neutral Density filter For intensity adjustment in photomicrography GIF Green Interference filter 546nm For phase contrast and contrast adjustment with black and white film Blue filter For general microscopy and colour photomicrography 9 Auto Off On switch function Pilot Lamp Fig 30 e The power control knob is located above the left focusing knob e When auto is selected the blue pilot lamp indicates that an IR sensor is activated Fig 30 If no user is detected in front of the microscope the microscope illumination will automatically switch off after 15 minutes When the user returns the illumination will start again Never attempt to switch directly between on and auto The buffer off is necessary between auto power off mode and normal mode 22 V Photomicrographic Procedure Only on AE2000 TRI To ensure vibration free operation set the microscope on a sturdy vibration free table or a bench with a vibration proof device Turn the beam splitter knob of the eyepiece tube to the photo position Fig 31 The photo exit is activated 80 of the light will enter the camera Select a blue filter for routine application An
9. wide field high eye point Nosepiece Quadruple inclined sidewards Stage Fixed Dimensions width x depth 200 x 239mm Stage height 192mm Object guide Verniers with numerical and alphabetic scale X direction numerical scale readable from right to left Y direction alphabetic scale Objectives Plan Achromat 4x N A 0 1 W D 12 6mm Plan Achromat 10x N A 0 25 W D 16 8mm LWD Plan Achromat 20x N A 0 3 W D 4 7mm LWD Plan Achromat 40x N A 0 5 W D 3mm Plan Achromat PH 4x PhO N A 0 1 W D 12 6mm Plan Achromat PH 10x Ph1 N A 0 25 W D 4 1mm LWD Plan Achromat PH 20x Ph2 N A 0 3 W D 4 7mm LWD Plan Achromat PH 40x Ph2 N A 0 5 W D 3mm Condenser N A 0 3 W D 72mm N A 0 4 W D 53mm N A 0 5 W D 28mm in prep Coarse focus 42mm rot Fine focus 0 2mm rot Transmitted 6V 30W Halogen illumination 3W LED Dimension W x Dx H 217 5 x 556 x 497mm ll Setting up the Instrument 1 Working Environment The location should be free from dust moisture chemical vapours and from mechanical vibrations Don t locate the instrument in bright or direct ambient light in front of a lamp or a will lit bright wall Best image will be achieved without significant ambient light Environmental specification e Indoor use e Altitude Max 2000m e Ambient temperature 15 C 40 C e Maximum relative humid
10. Motic AE2000 Series Inverted Microscope Instruction Manual A WWW MOTIC COM MOTIC INCORPORATION LTD CE Wes UL Listed Product E250223 We are constantly endeavouring to improve our instruments and to adapt them to the requirements of modern research techniques and testing methods This involves modification to the mechanical structure and optical design of our instruments Therefore all descriptions and illustrations in this instruction manual including all specifications are subject to change without notice Content Nomenclature 1 Application 2 Nomenclature 3 Technical data Setting up the Instrument 1 Working Environment 2 Input voltage and power Assembling the Microscope 1 Installing the bulb 1 1 Halogen bulb 1 2 LED module 2 Filter Holder 3 Mounting the Condenser 4 Installing the Objectives 5 Mechanical Stage 6 Inserting the Eyepieces Microscope Handling Interpupillary Distance Adjustment Diopter Adjustment Coarse and fine focusing Coarse focus torque adjustment Changing between Halogen and LED illumination Brightfield Microscopy Phase Contrast Microscopy Filter selection Auto Off On switch function oO ON Oa BA O N Photomicrographic Procedure Only on AE2000 TRI N A A A 00 10 10 12 12 12 13 13 14 15 16 16 16 17 17 18 19 19 22 22 23 VI Troubleshooting Table 1 Optical and Operating Problems 2 Electrical VII C
11. additional colour compensating filter can also be used depending on the colour rendition A change of depth of focus contrast and resolution of image is attainable with an aperture setting of about 2 3 maximum Fine setting of the condenser aperture is depending on the individual sample For photomicrographic procedures refer to the manual of the specific camera being used N Beam Splitter Knob Y Fig 31 23 VI Troubleshooting Table As you use your microscope you may occasionally experience a problem The troubleshooting table below contains the most frequently encountered problems and their possible causes 1 Optical and Operating Problems Problem Possible Cause Vignetting or uneven brightness in the field of view or field of view only partially visible Bulb not installed properly Filter slider in intermediate position Phase slider not in click stop position Incorrect condenser mounting Aperture diaphragm closed too far Revolving nosepiece not clicked into position Beam splitter knob not clicked into position Mod AE2000 trinocular only Dust or dirt in field of view Aperture diaphragm closed too far Dust or dirt on specimen s surface Inadequate image quality Brightfield objective used with Phase illumination Annular ring does not fit to objective phase ring Annular ring has to be readjusted Eye strain or fatigue Interpupillary distance n
12. are and Maintenance 1 Lenses and Filters 2 Cleaning of Painted or Plastic Components 3 When Not in Use 4 Warning Label 24 24 24 25 25 25 25 26 I Nomenclature 1 Application The new Inverted microscope AE2000 is representing the ideal instrument for living cells observation and other microbiological samples Due to our Colour Corrected Infinity Optics CCIS the AE2000 offers superior image quality combined with excellent mechanical performance Daily work is facilitated by a number of ergonomic features 2 Nomenclature Model AE2000 Lamp House Filter Holder Clamp Screw Eyepiece Phase Slider Condenser Stage Diopter Adjustment Ring Light Intensity Control Knob Model AE2000 1 5mm Hexagonal screwdriver Objective Nosepiece Auto Off On Switch Fine Focus Coarse Focus H e Coarse Focus Torque Adjustment Model AE2000 TRI Photo Port Beam Splitter Knob Model AE2000 Ergo ERGO Tube 3 Technical data Technical data AE2000 BIN AE2000 TRI AE2000 Ergo Optical system Color Corrected Infinity Optics CCIS Total Magnification 40X 400X Eyepiece tubes 360 Swiveling Siedentopf with upper and lower position upper position offers approx 40 mm extra viewing height Interpupillary distance 48 mm to 75 mm Binocular 45 Inclined Trinocular 45 Inclined Ergo Inclined 45 15 Eyepiece N WE 10X 920
13. bling the Microscope mba Installing the bulb e In order to prevent electric shock always turn the power switch off Fig 1 and unplug the power cord Fig 2 before replacing the bulb e To remove lamphouse cover press down lightly 1 and rotate counter clockwise 2 Fig 3 Power Cord Power Switch Fig 1 Power Cord Fig 2 Lamp house Cover Fig 3 10 e Firmly insert the bulb into the socket pinholes Fig 4 until it reaches the limit be careful not to tilt the lamp when mounting Fig 5 Halogen Bulb e When installing the halogen bulb be careful not to touch the glass surface of the bulb with bare fingers to avoid fingerprints grease etc Surface pollution can burn the bulb and reduce the illumination provided by bulb If surface is contaminated wipe it clean using lens tissue or soft cotton Clamp screw 1 5mm Hexagonal screwdriver Fig 7 LED module Firmly insert the LED module into the socket pinholes until it reaches the limit This is a Motic patented design to exchange LED module and halogen bulb on the same socket directly e After the LED module installation Fig 6 secure it with the clamp screw by 1 5mm hexagonal screwdriver supplied with the microscope Fig 7 e Return lamphouse cover to original position and rotate clockwise to lock into place The white paint marked on the cover should face the user Fig 8 11 Fig 8 1 1 Halogen bulb The quartz
14. ity 75 for temperature up to 31 C decreasing linearly to 50 relative humidity at 40 C e Supply voltage fluctuations Not to exceed 10 of the normal voltage e Pollution degree 2 in according with IEC60664 e Installation Overvoltage category 2 in according with IEC60664 e Air Pressure of 75kPa to 106 kPa 2 Input voltage and power Automatic voltage selection works with electrical outlets worldwide It is advised to always use a power cord that is rated for the voltage used in your area and that has been approved to meet local safety standards Using the wrong power cord could cause fire or equipment damage A In order to prevent electric fluctuation to the instrument electrics always turn the power switch on the instrument off before connecting the power cord This equipment must be used with UL60950 1 Listed power supply E246759 MFR LI TONG ELECTRONICS CO LTD Model LTE50E S2 3 e Input Voltage 12VDC e Input Power 48W e Power adaptor input rating 100 240Vac 47 63Hz 1A Attention The plug of the power adaptor is the disconnect device for whole unit To save energy we recommend to unplug the instrument when not in use This device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation lll Assem
15. ot adjusted Diopter adjustment not made Inadequate illumination 2 Electrical Problem Possible Cause Lamp does not light Power supply not plugged in Lamp not installed User left more than 15 minutes under auto mode Lamp burnt out Inadequate brightness Specified lamp not being used Lamp blows out immediately Specified lamp not being used Lamp flickers Connectors are not securely connected Lamp near end of life time Lamp not securely plugged into socket 24 Vil Care and Maintenance 1 Lenses and Filters e To clean lens surfaces or filters first remove dust using an air blower If dust still persists use a soft clean brush or gauze e A soft gauze or lens tissue lightly moistened with the mixture of alcohol and ether ratio alcohol 3 and ether 7 should only be used to remove grease or fingerprints e Use the mixture of alcohol and ether ratio alcohol 3 and ether 7 only to remove immersion oil from objective lenses e Because the mixture of alcohol and ether ratio alcohol 3 and ether 7 is highly flammable be careful handling around open flame e Do not use same area of gauze or lens tissue to wipe more than once 2 Cleaning of Painted or Plastic Components e Do not use organic solvents thinners alcohol ether etc Doing so could result in discolouration or in the peeling of paint e For stubborn dirt moisten
16. s in the nosepiece thread holes in a manner that the magnification increases with clockwise rotation of the revolving nosepiece Fig 15 e Place back the stage insert Fig 14 Fig 15 5 Mechanical Stage e An attachable mechanical stage is available as an option This stage allows the precise x Y movement of common vessels for cell culture For choosing the respective vessel carrier please contact your local Motic supplier e Secure the mechanical stage to the AE2000 plain stage using the two mounting screws Fig 16 located beneath the stage on the right side user facing the front of the instrument Mounting screw Fig 16 14 Inserting the Eyepieces Remove the dust caps from the eyepiece tubes Use the same magnification eyepieces for both the eyes Insert each eyepiece into the eyepiece sleeve by a twisting movement Fig 17 Should the rubber eye guards are to be used fit them in the groove around the eyepiece Fig 17 15 IV Microscope Handling Interpupillary Distance Adjustment Before adjusting the interpupillary distance bring a specimen into focus using the 10x objective Adjust the interpupillary distance so that both the right and left field of view become one This adjustment will enable the user to observe the specimen with both eyes 2 Diopter Adjustment Diopter adjustment compensates for the differences in vision between the left and right eyes In addition to making observation through
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