USER MANUAL - LA Biosystems
Contents
1. l6 6 2 Checking Drive Belt 17 Othar SERVICES 17 Section 7 Troubleshooting 18 Section 8 Reorder Information 19 Section 9 20 3 WARRANTY INFORMATION This manual is a guide for the use of the Omni Prep Multi Sample Homogenizer accessories Data herein has been verified and validated It is believed adequate for the intended use of the instrument If the instrument or procedures are used for purposes over and above the capabilities specified herein confirmation of the validity and suitability should be obtained otherwise Omni International does not guarantee results and assumes no obligation or liability This publication is not a license to operate under or a recommendation to infringe upon any process patents Notes cautions and warnings within the text of this manual are used to emphasize important and critical instructions This Omni International product is warranted to be free from defects in material and workmanship for a period of ONE YEAR from the date of delivery Omni International will repair or replace and return free of charge any part which is returned to its factory within said period transportation prepaid by user and which is found upon inspection to hav2. OMNI PREP MULTI SAMPLE HOMOGENIZER USER MANUAL OMNI PREP MULTI SAMPLE HOMOGENIZER OMNI INTERNATIONAL R e e N po ae ON VLL STOP WW A wp VA WA PROGRAM We PROGRAM 2 PROGRAM 3 MANUAL MODE Sy International The Homogenizer Company Rev A Warranty 3 Section Important Safeguards 4 Section 2 Omni Prep Homogenizer 5 2 1 Unpacking The Omni 6 2 2 Available Accessories eeeeeeeeee eee eee eee ene 7 2 3 Preparation for WSC m UTE 9 Section 3 Loading the Processing Rack 3 1 Loading Sample Tubes amp Tube Holders 10 3 2 Set Up Processing Rack with Tubes 11 3 3 Loading the Ice Bath 12 3 4 Installing the Processing 12 Section 4 Modes of Operation Program Mod 13 14 4 3 Processing Rack 14 Section 5 Cleaning Procedures 5 1 Cleaning the Omni 15 52 leanne OMN T I5 Section 6 Servicing the Omni Prep 6 1 Fuse Check and
3. SECTION I IMPORTANT SAFEGUARDS READ ALL INSTRUCTIONS BEFORE USING SAVE THIS USER MANUAL The Omni Prep has been engineered for maximum functionality as well as safety however basic safety precautions and common sense must always be demonstrated when using any electrical product Do not attempt to modify any part of the Omni Prep If you experience problems with or have questions about your Omni Prep contact your authorized dealer or call Omni at 800 776 4431 770 421 0058 WARNING DO NOT allow the machine to be submerged in any liquid DO NOT use in any setting other than an indoor laboratory DO NOT plug power cord into an incorrect outlet Keep this product away from heated surfaces To reduce the risk of burns electrocution fire or injury Use this product only for its intended purpose as described in this booklet Do not use attachments not recommended by the manufacturer DO NOT operate the product if it is damaged in any way RISK OF ELECTRIC SHOCK Although this equipment is fully insulated and grounded it is important for all users to be aware of the potential hazard of using liquids close to a power supply If any liquids are spilled immediately disconnect the instrument from the main power supply remove the power cord from the AC power input on the rear panel and clean the equipment and the surrounding area DO NOT reconnect the equipment until it as been fully inspected SECTION
4. 2 OMNI PREP HOMOGENIZER The Omni Prep Homogenizer is a variable speed multi sample homogenizer The Omni Prep is powered by a 150 watt 30 000 rpm belt driven motor It includes a built in high efficiency speed control a digital speed display and a built in timer The Omni Prep operates exclusively with Omni Tip plastic generator probes or specially designed Stainless Steel Probes The motor drive unit housing has been specifically designed to reduce motor noise to less than 75db at top speed The Omni Prep allows you to process up to 6 samples at a time 5mL 50mL sample tubes Belt Drive Door j Window Lift Processing Plate Floor Mat Linear Control Actuator Panel nacie TOEIC Thumb Tab Omni Tip Cradle Sample Tube 15mL shown Tube ed Dynamic _ eT Power Entry Module Processing Rack SECTION 2 OMNI PREP HOMOGENIZER 2 1 UNPACKING THE OMNI PREP Open the box on the floor Remove the two rack boxes Package may contain one rack box depending on your order 2 Remove the accessory box 3 Lay the box down on one side Make sure that the box flap is folded under arrow 4 Turn the box upside down making sure that all of the flaps are folded out as shown 5 Lift the box off of the foam as shown 6 Lay the Omni Prep upside down on the floor 7 Work the foam off of the unit by lifting from one sid
5. Stainless Steel Probes to touch the bottoms of the tubes CAUTION DO NOT allow the Omni Tips Stainless Steel Probes to ever be completely extracted from the sample tubes during processing 6 Remove the Omni Tips Stainless Steel Probes and the sample tubes 7 Load tubes containing sample and fluid and replace the Omni Tips Stainless Steel Probes The rack is now ready to be loaded into the Omni Prep Note If stationary operation is desired load the tube holders as described in section 2 1 and leave the processing rack toggle switch in the Off position during processing CAUTION DO NOT use sealed tube caps when moveable rack processing is desired Rack will not move with tube caps in place PLEASE NOTE Portion of Omni Tip Stainless Steel Probe shown below the red line must be submerged in liquid during processing 3 3 LOADING THE ICE BATH OPTIONAL Install the empty ice bath to the rack underneath the tube holders 2 Lower the tube holders so that the tubes are at the desired depth relative to the ice bath 3 Place the cooling agent chilled water ice dry ice etc into the bath 3 4 INSTALLING THE PROCESSING RACK Ensure the lever is positioned all the way to the left hold the rack in your right hand using the thumb tab located on the center of the top plate 2 Slide the rack onto the lift plate and push completely back until it stops 3 Close the door and sweep the lever to the right until the proce
6. has been installed see section 3 and the Program to be run has been specified see sections 4 1 and 4 2 press the START button and turn on the movable rack toggle switch SECTION 5 CLEANING PROCEDURES 5 1 CLEANING THE OMNI PREP Remove power Remove the rack and the floor mat and wipe with a mild detergent and water solution The Omni Prep motor unit and tube holders may be wiped down with a damp cloth of the same mild detergent Do not use an excess amount of cleaning solution Dry thoroughly CAUTION DO NOT attempt to submerge any part of the Omni Prep in any solution DO NOT spray cleaning solution on to the Omni Prep Wipe the surfaces with a damp cloth DO NOT sterilize plastic tube holders in an autoclave They may be damaged if autoclaved 5 2 CLEANING OMNI TIPS While Omni Tips can be autoclaved up to seven times for repeated use they are not recommended for extended use with phenol or guanidine based reagents Disposal may be required when used in conjunction with these reagents However for many applications the probe can be cleaned and or sterilized for reuse by the procedures described below Not Recommend Autoclave up to 250 F e UV Irradiation Ethylene Oxide Formaldehyde Gluteralhyde 2 Gamma irradiation up to 5MRAD Sterilization Techniques Alcohol 7076 Phenolic Derivatives Hypochlorite 5 Extended exposure to chlorine bleach Guanidine Formaldehyde 40 Dichlorome
7. 00 Omni Deaggregation Tips Order 34750 500 Omni Deaggregation Tips Order 35750 25 Omni Hard Tissue Tips Order 30750H 50 Omni Hard Tissue Tips Order 32750H 100 Omni Hard Tissue Tips Order 34750H 500 Omni Hard Tissue Tips Order 35750H See page 8 for additional accessories SECTION 2 OMNI PREP HOMOGENIZER 2 2 AVAILABLE ACCESSORIES cont Stainless Steel Homogenizer Probes These Stainless Steel Probes were designed specifically for the Omni Prep These probes are ideal for larger sample volumes larger initial sample tissue sizes and tougher tissue types Stainless Steel Probes are also resistant to most organic solvents commonly used during homogenization bmm x 75mm Stainless Steel Probe Order B5 075 6 Pack Order B5 075 6 7mm x llO0mm Stainless Steel Probe Order B7 110ST 6 Pack Order B7 110ST 6 x 110mm Stainless Steel Probe Order BI0 I1 10ST 6 Pack Order 10 110 6 7mm Omni Tip Hybrid Probes The Omni Tip Hybrid Probes combine the convenience of disposable plastic probes with the durability of traditional stainless steel probes Hybrid probes are made up of an outer stainless steel tube with an inner Ultem plastic shaft The plastic shafts can either be disposed of after use or cleaned and reused multiple times The simple two piece design makes these probes much easier to clean and disassemble than traditional stainless steel probes 7m
8. E certified CE certified 21 Animal Human Cellular Plant Food Chemistry Our Applications database can help you find the best solution quickly Our constantly expanding database consists of carefully reviewed customer published applications sorted into useful categories complete with product recommendations to help you find the right laboratory homogenizer for any sample type If you can t find an answer in the database call us and we ll help www omni inc com applications P d e p Mechanical Ultrasonic Bead Automation Shear Mills Robotic NY 22 NOTES NOTES 23 24 OMNI International 935 C Cobb Place Blvd Toll Free 1 800 776 4431 Kennesaw GA 30144 Phone 770 421 0058 Email omni omni inc com Patents Pending P N 03 201 www omni inc com
9. e been defective in materials or workmanship For the first 90 days both parts and service are without charge For the balance of the period parts will be provided but service will be charged at established labor rates This warranty does not include normal wear from use it does not apply to any instrument or parts which have been altered by anyone other than an employee of Omni International nor to any instrument which has been damaged through accident negligence failure to follow operating instructions the use of electric currents or circuits other than those specified on the plate affixed to the instrument misuse or abuse Omni International reserves the right to change alter modify or improve any of its instruments without any obligation whatever to make corresponding changes to any instrument previously sold or shipped THE FORGOING OBLIGATION IS IN LIEU OF ALL OBLIGATIONS AND LIABILITIES INCLUDING NEGLIGENCE AND ALL WARRANTIES OF MERCHANTABILITY OR OTHERWISE EXPRESSED OR IMPLIED IN FACT OR BY LAW AND STATE OUR ENTIRE AND EXCLUSIVE LIABILITY AND BUYERS EXCLUSIVE REMEDY FOR ANY CLAIM OF DAMAGES IN CONNECTION WITH THE SALE OR FURNISHING OF GOODS OR PARTS THEIR DESIGN SUITABILITY FOR USE INSTALLATION OR OPERATION OMNI INTERNATIONAL WILL IN NO EVENT BE LIABLE FOR ANY SPECIAL OR CONSEQUENTIAL DAMAGES WHATSOEVER AND THEIR LIABILITY UNDER NO CIRCUMSTANCES WILL EXCEED THE CONTRACT PRICE FOR THE GOODS FOR WHICH LIABILITY IS CLAIMED
10. e to the other as shown The unit is held very tightly in the foam 8 Turn the unit over and remove the rest of the foam PLEASE NOTE SAVE ALL INCLUDED PACKAGING SECTION 2 OMNI PREP HOMOGENIZER 2 2 AVAILABLE ACCESSORIES The Omni Prep is sold as a customizable homogenizing system Listed below are all available accessories for the Omni Prep At least processing rack 3 tube holders and Omni Tips are required to operate the Omni Prep Omni Prep Processing Rack Order 06 220 Movable for highly efficient process Required for operating the Omni Prep Omni Prep Rack Tube Holders Tube holders are available for 1 5 2mL 5mL 15 and 50mL sample tubes Custom tube holder sizes are also available Each holder holds 2 sample tubes Tube holders are required to operate the Omni Prep Rack Tube Holder for 5mL to 2mL Order 06 209 02 Rack Tube Holder for 5mL Order 06 209 05 Rack Tube Holder for 15mL Order 06 209 15 Rack Tube Holder for 50mL Order 06 209 50 Omni Tip Plastic Homogenizer Probes Omni Tips are designed to completely avoid cross contamination between samples They are engineered from a durable plastic and come in two different configurations for soft tissue and hard tissue Omni tips are economical to allow for disposal after each use or they can be cleaned and autoclaved up to seven times 25 Omni Deaggregation Tips Order 30750 50 Omni Deaggregation Tips Order 32750 1
11. et Call if problem persists Omni Prep begins a run but Omni Tips are Drive Belt may need servicing Section 6 2 not rotating Turn the Omni Prep OFF and call Omni technical service 800 776 443 Rack raises but will not lock Do Not Force Lever Verify that the rack is pushed completely onto lift plate Damaged drive connects on Omni Tip shafts replace the Omni Tips Rack binds in transition to locked position Do Not Force Lever Omni Tip drive connects may be damaged Inspect and replace if necessary Verify that rack is securely pushed into place see Section 3 Omni Tips stick in Omni Prep drives when Omni Tip drive connect worn replace Omni unlocking the rack Tip Excessive noise vibration from Omni Tips Fluid level may be too low in tube Drive Belt may need servicing Section 6 2 Excessive splashing in sample tube Fluid level too low for tube size Contact Omni about sealed tube operation 19 SECTION 8 REORDER INFORMATION This table provides part numbers for Omni Prep replacement parts Please call 1 800 776 4431 to order Ice Tray for I5mL 50mL Tube Sizes 20 SECTION 9 SPECIFICATIONS 9 1 SPECIFICATIONS Variable Motor Speed Speed Control Accuracy Dimensions Weight Electrical Requirements Standards Approval Compliance 15V 220V 500 30 000rpm 1 40cm x 20cm x 30cm 15 7 in x 7 8 in x 11 8 21 Ibs 9 53kg 15 volts 60Hz or 220 volts 50Hz C
12. he rack leaving the rods on the far ends empty 50mL holders shown If six samples are desired mount all three tube holders in the rack as shown 50mL tube holders shown 2 Load empty sample tubes into tube holders Do not add sample or fluid to tubes until after a complete dry setup has been made 50 tube holders shown 3 Install Omni Tips or Stainless Steel Probes by first inserting the lower end into the sample tube then lower the Tip Probe until it rests securely in the Omni Tip cradle CAUTION DO NOT allow Omni Tips to touch the bottoms of the tubes CAUTION Only Omni Tips made by Omni International are compatible with the Omni Prep Attempting to use other style processors will damage the Omni Prep and void all warranties 3 2 SET UP PROCESSING RACK WITH TUBES The processing rack has been engineered to allow the samples to be vertically oscillated relative to the probes during processing Load the tube holders sample tubes and Omni Tips Stainless Steel Probes by following the steps in section 2 1 50mL tubes shown 2 Loosen top adjustment knob on rear of rack 3 Raise platform up to desired Tip Probe depth in the sample tubes Lower top adjustment knob to platform and tighten 4 Loosen lower adjustment knob on rear of rack 5 Move platform down to desired Tip Probe maximum height in sample tubes Raise lower adjustment knob to meet platform and tighten CAUTION DO NOT allow Omni Tips
13. he speed may be adjusted during a run but the time may not The time will count down from the stored value Processing may be paused at any time by pressing the STOP button once or by lowering the rack the message line will read RUN PAUSED Pressing START will resume the run the message line will read RUN RESUMED rack must be locked Pressing the STOP button twice will cancel the run and reset the time to the starting value SECTION 4 MODES OF OPERATION 4 2 MANUAL MODE The speed value is actively stored and may be changed at any time Press START to begin processing Processing may be paused at any time by pressing the STOP button once or by lowering the rack the LCD display will read RUN PAUSED Pressing START will resume the run the display will read RUN RESUMED rack must be locked Pressing the STOP button twice will cancel the run and reset the time to zero 4 3 PROCESSING RACK OPERATION The processing rack is controlled by the toggle switch on the side of the Omni Prep The mechanism raises and lowers the rack as the samples are processed allowing more even efficient processing When in use the rack oscillates through the user specified range of motion see section 3 2 The maximum range of motion is about 2 inches The rack oscillates at an approximate rate of 0 8 Hz The system is disengaged until the rack is raised into the running position by sweeping and locking the lever to the right Once the rack
14. m x ll0mm Hybrid Probe Order 22 007 6 Pack Order 22 007 6 Omni Prep Tray Keeps frozen and sensitive samples cold during processing Ice Tray for l 5mL 2mL Tubes Order 06 207 Ice Tray for IbmL 50mL Tubes Order 06 210 For more information or to order please contact Omni at 1 800 776 443 or visit www omni inc com SECTION 2 OMNI PREP HOMOGENIZER 2 3 PREPARATION FOR USE Follow the instructions in Section 1 1 to remove the Omni Prep and all accessories from their packaging and place the instrument on a hard flat surface Connect the power cord to the power entry module on the rear panel and plug it into an appropriate outlet Note The Omni Prep is equipped with a universal power supply which works with an input voltage of 10V 230V AC at 50 or 60Hz ET SEN K AIS 257 VEU N S AZ SE N N SES 4 e A or NN 5 SN Ss AEN SECTION 3 LOADING THE PROCESSING RACK 3 1 LOADING SAMPLE TUBES AND TUBE HOLDERS The processing rack has been engineered to hold up to six open or sealed tubes of various sizes Determine the number of samples to be processed Only an even numbers of samples may be processed at a time If processing only two samples place the tube holder between the two middle bars as shown 50mL tube holder shown If processing only four samples mount the tube holders on t
15. ssing rack locks in its raised position CAUTION The lever should move with smooth and constant motion If the lever does not slide smoothly as it is moved to the right Slide the lever back to the left 2 Remove the rack 3 Rotate the tops of the Omni Tip shafts 4 Replace the rack in the Omni Prep Repeat this procedure until the locking lever can be moved smoothly all the way to the right fully locked position SECTION 4 MODES OF OPERATION The Omni Prep is now ready for use Turn ON the power switch located on the rear of the unit rH LJ OMNI TAE EE 30000 RPM 1 30 MIN OMNI INTERNATIONAL Mode Line VA VA Message Line SPEED TIME PROGRAM 1 The Omni Prep has two modes of operation In Program mode PROGRAM the active program defines the process time and speed Processing time counts down and processing stops when time MANUAL MODE o PROGRAM 3 5 reaches zero In Manual Mode the timer counts UP from zero 229 OMNI INC COM and the Omni Prep continues to process until the Stop button is pressed 10 minute maximum 4 1 PROGRAM MODE The Omni Prep can store up to three separate programs The active program number is displayed on the mode line of the LCD screen example PROGRAM 1 A program is modifiable when it is active and the Omni Prep is not running Modified time and speed values are automatically stored in the active program Press START to begin processing t
16. thane Biological Disinfection Radiacwash Count Off Water Ethanol SDS Radioactive Decontamination SECTION 6 SERVICING THE OMNI PREP 6 1 FUSE CHECK AND REPLACEMENT Remove power Unplug the cord from the power entry module The fuse holder is located in the center of the power entry module 2 Use the tab on the right hand side of the fuse holder and screwdriver figure to slide the holder straight back until it is completely removed 3 Check the fuse by examining the filament in the center of the tube as shown figure B If the filament is broken continue to step four If the filament is in tact return the assembly into the power entry module and call Omni Technical Service 1 800 776 4431 4 Unclip the expired fuse and discard Press on one end of the enclosed portion of the fuse holder to remove the spare fuse from the casing as shown figure C Replace the empty casing into the fuse holder 5 Clip the new fuse into the exposed side of the holder and reinstall into the power entry module 6 Contact OMNI INTERNATIONAL to obtain a new replacement fuse Store the fuse in the casing on the enclosed side of the fuse holder Figure A SECTION 6 SERVICING THE OMNI PREP 6 2 CHECKING DRIVE BELT TENSION The Omni Prep operates by means of a belt driven motor system Over time the tension of the belt may need to be adjusted or the belt itself may need to be replaced The Omni Prep is eq
17. uipped with a Belt Drive Window located on top of the unit This window allows for easy viewing of the Belt Drive Tensioner The belt tension is correct when the tensioner is located between the letters B and C on the tension scale See figure below gt mea a Te gt i Tensioner If the belt tension is incorrect contact Omni International at 800 776 4431 for service options 6 3 ALL OTHER SERVICING For all other service information please contact OMNI INTERNATIONAL at 800 776 443 CAUTION ANY ATTEMPT TO REMOVE ANY SURFACE OR MODIFY THE OMNI PREP IN ANY WAY WILL VOID ANY WARRANTY WARNING To reduce the risk of electric shock personal injury or death DO NOT ATTEMPT TO REMOVE THE REAR PANEL OF THE OMNI PREP SECTION 7 TROUBLESHOOTING Do not attempt to service the Omni Prep in a manner other than those discussed in this manual For any issue that is unsuccessfully corrected using this guide please contact your authorized dealer or call technical assistance at Omni International at 1 800 776 4431 or 770 421 0058 PROBLEM CORRECTIVE ACTION The Omni Prep is plugged in and turned on Check power cord connectors but is not functioning Check wall socket for power to the outlet Check Fuse see Section 6 1 LCD screen is lit but motor will not run Verify that the LCD displays READY Make sure the rack is locked see Section 3 LCD lit but not functioning properly Turn power off and back on to res
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