PCR clean-up
Contents
1. e g Square well Block 96 well block with 2 1 mL square wells Elution plate for collecting purified 740486 24 24 nucleic acids e g Elution Plate U bottom 96 well 0 3 mL microtiterplate with 300 uL u bottom wells For preparation of working solutions and storage conditions see section 3 4 MACHEREY NAGEL 08 2012 Rev 02 PCR clean up 2 2 1 Product description The basic principle The NucleoMag 96 PCR procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions Adjusting the PCR reaction to binding conditions and addition of paramagnetic beads can be carried out simultaneously After magnetic separation and removal of supernatant the beads are washed to remove contaminants and salt A short drying step is necessary to remove ethanol from previous washing steps Finally highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications The NucleoMag 96 PCR kit can be used either manually or automated on standard liquid handling instruments 2 2 Kit specifications NucleoMag 96 PCR is designed for rapid manual and automated clean up of PCR fragments using the NucleoMag SEP Magnetic Separator see ordering information other magnetic separation systems see section 2 3 Manual processing time for 96 samples is about 45 min NucleoMag 96 PCR is easily adapted to common liquid handl2. 740481 Tecan Te MagS 1 5 mL tubes without lid Sarstedt Static magnetic pins Separators with static magnetic pins for example NucleoMag SEP for manual use and for use on liquid handling workstations This type of separator is recommended in combination with a suitable microplate shaker for optimal resuspension of the beads during the washing and elution steps Alternatively beads can be resuspended in the buffer by pipetting up and down several times For fully automated use on liquid handling workstations a gripper tool is required the plate is transferred to the magnetic separator for separation of the beads and transferred to the shaker module for resuspension of the beads Movable magnetic systems Separators with moving magnetic pins Magnetic pins rods are moved from one side of the well to the other and vice versa Beads follow this movement and are thus pulled through the buffer during the wash and elution steps Separation takes place when the system stops Automated separators Separators with moving magnets Magnetic beads are transferred into suitable plates or tubes Beads are resuspended from the rod covered magnets Following binding washing or elution beads are collected again with the rod covered magnets and transferred to the next plate or tube 6 MACHEREY NAGEL 08 2012 Rev 02 PCR clean up 2 4 Adjusting the shaker settings When using a plate shaker for the washing and elution steps the
3. EREY NAGEL 08 2012 Rev 02 PCR clean up Problem Possible cause and suggestions Time for magnetic separation too short Increase separation time to allow the beads to be attracted to the magnetic pins completely Carry over of beads Aspiration speed too high elution step High aspiration speeds during the elution step may cause bead carry over Reduce aspiration speed for elution step 6 2 Ordering information Product REF Pack of NucleoMag 96 PCR 744100 1 1 x 96 preps 744100 4 4 x 96 preps 744100 24 24 x 96 preps NucleoMag SEP 744900 1 Square well Blocks 740481 4 740481 24 24 Elution Plate U bottom 740486 24 24 Self adhering PE Foil 740676 50 sheets Visit www mn net com for more detailed product information MACHEREY NAGEL 08 2012 Rev 02 19 PCR clean up 6 3 Product use restriction warranty NucleoMag 96 PCR kit components are intended developed designed and sold FOR RESEARCH PURPOSES ONLY except however any other function of the product being expressly described in original MACHEREY NAGEL product leaflets MACHEREY NAGEL products are intended for GENERAL LABORATORY USE ONLY MACHEREY NAGEL products are suited for QUALIFIED PERSONNEL ONLY MACHEREY NAGEL products shall in any event only be used wearing adequate PROTECTIVE CLOTHING For detailed information please refer to the respective Material Safety Data Sheet of the product MACHEREY NAGEL products shall exclusively be use
4. N Einige Minuten lang behutsam mit Wasser sp len Vorhandene Kontaktlinsen nach M glichkeit entfernen Weiter aussp len P 330 Rinse mouth Mund aussp len P 337 313 Get medical advice attention Bei anhaltender Augenreizung rztliche Rat einholen rztliche Hilfe hinzuziehen For further information please see Material Safety Data Sheets www mn net com Weiterf hrende Informationen finden Sie in den Sicherheitsdatenbl ttern www mn net com MACHEREY NAGEL 08 2012 Rev 02 11 NucleoMag 96 PCR 5 Protocol for clean up of 50 pL PCR reactions Protocol at a glance For additional equipment and hardware requirements refer to section 1 2 and 2 3 respectively For detailed information on each step see page 17 Before starting the preparation Check if Buffer MP3 was prepared according to section 3 1 Transfer PCR 50 uL PCR reaction reaction mixture to appropriate 96 well For PCR reactions lt 50 uL adjust the volume to 50 uL plate using sterile water 2 Bind DNA to 12 uL NucleoMag P Beads NucleoMag P Beads 138 uL MP1 Mix by shaking for 5 min at RT Optional Mix by pipetting up and down Remove supernatant after 2 min separation 3 Wash with MP2 Remove Square well Block from NucleoMag SEP 300 pL MP2 Resuspend Shake 5 min at RT Optional Mix by pipetting lt _ gt up and down 12 MACHEREY NAGEL 08 2012 Rev 02 NucleoMag 96 PCR
5. PCR clean up User manual NucleoMag 96 PCR August 2012 Rev 02 MACHEREY NAGEL MN PCR clean up Table of contents 1 Components 1 1 Kit contents 1 2 Equipment and consumables to be supplied by user 2 Product description 2 1 The basic principle 2 2 Kit specifications 2 3 Magnetic separation systems 2 4 Adjusting the shaker settings 2 5 Handling of beads 2 6 Elution procedures 3 Storage conditions and preparation of working solutions 4 Safety instructions 4 1 Risk and safety phrases 4 2 GHS classification 5 Protocol for clean up of 50 uL PCR reactions 6 Appendix 6 1 Troubleshooting 6 2 Ordering information 6 3 Product use restriction warranty gt NN O9 a wo 10 10 11 12 18 18 19 20 MACHEREY NAGEL 08 2012 Rev 02 PCR clean up 1 Components 1 1 Kit contents NucleoMag 96 PCR 1x 96 preps 4 x 96 preps 24 x 96 preps REF 744100 1 744100 4 744100 24 NucleoMag P Beads 1 4 mL 5 6 mL 33 6 mL Binding Buffer MP1 16 mL 64 mL 384 mL Wash Buffer MP2 36 mL 2x72mL 864 mL Wash Buffer MP3 15 mL 60 mL 2x 180 mL Concentrate Elution Buffer MP4 75 mL 30 mL 180 mL Elution Plate U bottom 1 4 24 including Self adhering PE Foil User manual 1 1 1 1 2 Equipment and consumables to be supplied by user Product REF Pack of Magnetic separation system 744900 1 e g NucleoMag SEP see section 2 3 Separation plate for magnetic beads 740481 4 separation 740481 24 24
6. Remove supernatant after 2 min separation 4 1 wash with MP3 Remove Square well Block from NucleoMag SEP 300 pL MP3 Resuspend Shake 5 min at RT Optional Mix by pipetting up and down Remove supernatant after 2 min separation 5 214 wash with MP3 Remove Square well Block from NucleoMag SEP 300 pL MP3 Resuspend Shake 5 min at RT Optional Mix by pipetting up and down Remove supernatant after 2 min separation 6 Dry the beads 10 min at RT 7 Elute DNA Remove Square well Block from NucleoMag SEP 25 100 pL MP4 Optional Elute at 55 C MACHEREY NAGEL 08 2012 Rev 02 13 NucleoMag 96 PCR Shake 5 min at RT Optional Mix by pipetting up and down Separate 2 min and transfer DNA into Elution Plate U bottom 14 MACHEREY NAGEL 08 2012 Rev 02 NucleoMag 96 PCR Detailed protocol This protocol is designed for magnetic separators with static pins e g NucleoMag SEP and suitable plate shakers see section 2 3 It is recommended using a Square well Block for separation see section 1 2 Alternatively isolation of DNA can be performed in reaction tubes with suitable magnetic separators This protocol is for manual use and serves as a guideline for adapting the kit to robotic instruments Before starting the preparation Check if Buffer MP3 was prepared according to section 3 1 Tr
7. ansfer PCR reaction mixture Transfer PCR reaction mixture to an appropriate 96 well plate For PCR reaction volumes lt 50 uL adjust the volume to 50 uL using sterile water Note See recommendations for suitable plates e g Square well Block not included in the kit and compatible magnetic separators section 2 3 2 Bind DNA to NucleoMag P Beads Add 12 uL NucleoMag P Beads and 138 uL Binding Buffer MP1 to each well of the separation plate Mix by pipetting up and down 6 times and shake for 5 min at room temperature Alternatively when processing the kit without a shaker pipette up and down 10 times and incubate for 5 min at room temperature Note NucleoMag P Beads and Binding Buffer MP1 may be premixed For 96 samples premix 1248 uL of NucleoMag P Beads with 14 35 mL of Buffer MP1 mix by vortexing Use 150 uL of the suspension per well Be sure to resuspend the NucleoMag P Beads before removing them from the storage bottle Vortex storage bottle briefly until a homogenous suspension has been formed Separate the magnetic beads against the side of the wells by placing the 96 well plate on the NucleoMag SEP magnetic separator Wait at least 2 min until all the beads have been attracted to the magnets Remove and discard supernatant by pipetting Note Do not disturb the attracted beads while aspirating the supernatant The magnetic pellet is not visible in this step Remove supernatant from the opposite side of the we
8. buffer suspension at 55 C for 5 min MACHEREY NAGEL 08 2012 Rev 02 17 PCR clean up 6 Appendix 6 1 Troubleshooting Problem Possible cause and suggestions Poor DNA yield Wash Buffer MP3 did not contain ethanol Addition of the the indicated volume of 96 100 ethanol to Buffer MP3 Concentrate is required before use Elution buffer volume insufficient Bead pellet must be covered completely with elution buffer Insufficient performance of elution buffer during elution step Remove residual wash buffers during the separation steps completely Remaining buffers decrease efficiency of following wash steps and elution step Beads overdried Do not dry beads longer than 20 min at room temperature Overdrying of beads may result in lower elution efficiencies Suboptimal performance of DNA in downstream applications Carry over of ethanol from Wash Buffer MP3 Be sure to remove all of the ethanolic Wash Buffer MP3 after the final wash step Dry beads 10 15 min at room temperature Elution of DNA with TE buffer Use supplied elution buffer or sterile water Do not use TE buffer EDTA may inhibit sequencing reactions Repurify or precipitate DNA by ethanol and elute redissolve in Elution Buffer MP 4 buffer or water Eluted DNA contains residual primers primer dimers Minimize amount of primers in PCR reaction mixture Do not use higher volumes of binding buffer than specified 18 MACH
9. d in an ADEQUATE TEST ENVIRONMENT MACHEREY NAGEL does not assume any responsibility for damages due to improper application of our products in other fields of application Application on the human body is STRICTLY FORBIDDEN The respective user is liable for any and all damages resulting from such application DNA RNA PROTEIN purification products of MACHEREY NAGEL are suitable for IN VITRO USES ONLY ONLY MACHEREY NAGEL products specially labeled as IVD are also suitable for IN VITRO diagnostic use Please pay attention to the package of the product IN VITRO diagnostic products are expressly marked as IVD on the packaging IF THERE IS NO IVD SIGN THE PRODUCT SHALL NOT BE SUITABLE FOR N VITRO DIAGNOSTIC USE ALL OTHER PRODUCTS NOT LABELED AS IVD ARE NOT SUITED FOR ANY CLINICAL USE INCLUDING BUT NOT LIMITED TO DIAGNOSTIC THERAPEUTIC AND OR PROGNOSTIC USE No claim or representations is intended for its use to identify any specific organism or for clinical use included but not limited to diagnostic prognostic therapeutic or blood banking It is rather in the responsibility of the user or in any case of resale of the products in the responsibility of the reseller to inspect and assure the use of the DNA RNA protein purification products of MACHEREY NAGEL for a well defined and specific application MACHEREY NAGEL shall only be responsible for the product specifications and the performance range of MN products according to the
10. ds by placing the Square well Block on the NucleoMag SEP magnetic separator Wait at least 2 min until all the beads have been attracted to the magnet Remove and discard supernatant by pipetting Dry the beads Dry the beads by incubating the Square well Block 10 min at room temperature with the particles held against the magnet in order to allow the remaining traces of alcohol to evaporate Note Allow the pellet to dry sufficiently so that there is no visible droplets of buffer in the bottom of the tube Allowing the pellet to dry completely indicated by visible cracking Do not overdry beads e g by prolonged drying at 55 C This will reduce yield 16 MACHEREY NAGEL 08 2012 Rev 02 NucleoMag 96 PCR Elute DNA Remove the Square well Block from the NucleoMag SEP magnetic separator Add desired volume of Elution Buffer MP4 25 100 uL to each well and resuspend the by shaking 5 10 min at 56 C Alternatively resuspend beads completely by repeated pipetting up and down and incubate for 5 10 min at room temperature Separate the magnetic beads by placing the Square well Block on the NucleoMag SEP magnetic separator Wait at least 2 min until all the beads have been attracted to the magnets Transfer the supernatant containing the purified PCR products to the Elution Plate U bottom Note Yield can be increased by 15 20 by using pre warmed elution buffer 55 C or by incubating the bead elution
11. event shall MACHEREY NAGEL be liable for claims for any other damages whether direct indirect incidental compensatory foreseeable consequential or special including but not limited to loss of use revenue or profit whether based upon warranty contract tort including negligence or strict liability arising in connection with the sale or the failure of MACHEREY NAGEL products to perform in accordance with the stated specifications This warranty is exclusive and MACHEREY NAGEL makes no other warranty expressed or implied The warranty provided herein and the data specifications and descriptions of this MACHEREY NAGEL product appearing in MACHEREY NAGEL published catalogues and product literature are MACHEREY NAGEL s sole representations concerning the product and warranty No other statements or representations written or oral by MACHEREY NAGEL s employees agent or representatives except written statements signed by a duly authorized officer of MACHEREY NAGEL are authorized they should not be relied upon by the customer and are not a part of the contract of sale or of this warranty Product claims are subject to change Therefore please contact our Technical Service Team for the most up to date information on MACHEREY NAGEL products You may also contact your local distributor for general scientific information Applications mentioned in MACHEREY NAGEL literature are provided for informational purposes only MACHEREY NAGEL does not
12. ing instruments The actual processing time and sample volume to be processed depends on the configuration of your instrument and the used magnetic separation system Typically 96 samples can be purified in about 30 45 min The kit provides reagents for the purification of 3 5 ug DNA from 50 uL PCR samples Typical concentration of the purified PCR samples of 75 200 ng uL can be achieved The purity of recovered PCR products is Asgo Azgo gt 1 7 1 9 The kit is designed for use with or without detergent containing PCR buffers Purified PCR products are ready to use for downstream applications like automated fluorescent sequencing labeling microarray analysis cloning or restriction digestion NucleoMag 96 PCR can be processed completely at room temperature Elution at 55 C will increase the recovery by about 10 15 NucleoMag P Beads are highly reactive superparamagnetic beads The binding capacity is 0 3 ug of DNA per 1 uL of NucleoMag P Bead suspension 1 uL of suspension contains 150 ug of beads MACHEREY NAGEL 08 2012 Rev 02 5 PCR clean up 2 3 Magnetic separation systems For use of NucleoMag 96 PCR the use of the magnetic separator NucleoMag SEP is recommended Separation is carried out in a Square well Block see ordering information The kit can also be used with other common separators Magnetic separator Separation plate or tube NucleoMag SEP MN REF 744900 Square well Block MN REF
13. ll MACHEREY NAGEL 08 2012 Rev 02 15 NucleoMag 96 PCR Wash with MP2 Remove the Square well Block from the NucleoMag SEP magnetic separator Add 300 uL Buffer MP2 to each well and resuspend the beads by shaking until the beads are resuspended completely 5 min Alternatively resuspend beads completely by repeated pipetting up and down 15 times Separate the magnetic beads by placing the Square well Block on the NucleoMag SEP magnetic separator Wait at least 2 min until all the beads have been attracted to the magnet Remove and discard supernatant by pipetting 1 wash with MP3 Remove the Square well Block from the NucleoMag SEP magnetic separator Add 300 uL Buffer MP3 to each well and resuspend the beads by shaking until the beads are resuspended completely 5 min Alternatively resuspend beads completely by repeated pipetting up and down 15 times Separate the magnetic beads by placing the Square well Block on the NucleoMag SEP magnetic separator Wait at least 2 min until all the beads have been attracted to the magnet Remove and discard supernatant by pipetting 2t wash with MP3 Remove the Square well Block from the NucleoMag SEP magnetic separator Add 300 uL Buffer MP3 to each well and resuspend the beads by shaking until the beads are resuspended completely 5 min Alternatively resuspend beads completely by repeated pipetting up and down 15 times Separate the magnetic bea
14. per bottle below 25g or mL certificate of exemption according to 67 548 EEC Art 25 1999 45 EC Art 12 and German GefStoffV 20 3 and TRGS 200 7 1 For further information see Material Safety Data Sheet 10 MACHEREY NAGEL 08 2012 Rev 02 PCR clean up 4 2 GHS classification Only harmful features do not need to be labeled with H and P phrases until 125 mL or 125 g Mindergef hrliche Eigenschaften m ssen bis 125 mL oder 125 g nicht mit H und P S tzen gekennzeichnet werden Component Hazard contents GHS symbol Hazard Precaution phrases phrases Inhalt Gefahrstof GHS Symbol H S tze P S tze MP1 Guanidine hydrochloride Warning 302 319 280 301 312 36 50 305 351 338 330 Guanidinhydrochlorid 36 50 Achtung 337 313 MP2 Guanidine hydrochloride Warning 302 301 312 330 24 36 Guanidinhydrochlorid 24 36 Achtung Hazard phrases H 302 Harmful if swallowed Gesundheitssch dlich bei Verschlucken H 319 Causes serious eye irritation Verursacht schwere Augenreizung Precaution phrases P 280 Wear protective gloves eye protection Schutzhandschuhe Augenschutz tragen P 301 312 IF SWALLOWED Call a POISON CENTER or doctor physician if you feel unwell BEI VERSCHLUCKEN Bei Unwohlsein GIFTINFORMATIONSZENTRUM oder Arzt anrufen P 305 351 313 IF IN EYES Rinse continuously with water for several minutes Remove contact lenses if present and easy to do continue rinsing BEI KONTAKT MIT DEN AUGE
15. s 24 x 96 preps REF 744100 1 744100 4 744100 24 Buffer MP3 15 mL 60 mL 2x 180 mL Concentrate Add 60 mL ethanol Add 240 mL ethanol Add 720 mL ethanol to each vial MACHEREY NAGEL 08 2012 Rev 02 9 PCR clean up 4 Safety instructions The following components of the NucleoMag 96 PCR kits contain hazardous contents Wear gloves and goggles and follow the safety instructions given in this section 4 1 Risk and safety phrases Component Hazard contents Hazard Risk Safety symbol phrases phrases Inhalt Gefahrstoff Gefahrstoff R S tze S S tze symbol MP1 Guanidine hydrochloride 36 50 x Xn R 22 36 S 26 39 Guanidinhydrochlorid 36 50 xi MP2 Guanidine hydrochloride 24 36 x m R 22 Guanidinhydrochlorid 24 36 Risk phrases R22 Harmful if swallowed Gesundheitssch dlich beim Verschlucken R 36 Irritating to eyes Reizt die Augen Safety phrases S26 In case of contact with eyes rinse immediately with plenty of water and seek medical advice Bei Ber hrung mit den Augen sofort gr ndlich mit Wasser absp len und Arzt konsultieren S39 Wear eye face protection Schutzbrille Gesichtsschutz tragen Hazard labeling not necessary if quantity per bottle below 125g or mL certificate of exemption according to 67 548 EEC Art 25 1999 45 EC Art 12 and German GefStoffV 20 3 and TRGS 200 7 1 For further information see Material Safety Data Sheet Hazard labeling not necessary if quantity
16. specifications of in house quality control product documentation and marketing material This MACHEREY NAGEL product is shipped with documentation stating specifications and other technical information MACHEREY NAGEL warrants to meet the stated specifications MACHEREY NAGEL s sole obligation and the customer s sole remedy is limited to replacement of products free of charge in the event products fail to perform as warranted Supplementary reference is made to the general business terms and conditions of MACHEREY NAGEL which are printed on the price list Please contact us if you wish to get an extra copy There is no warranty for and MACHEREY NAGEL is not liable for damages or defects arising in shipping and handling transport insurance for customers excluded or out of accident or improper or abnormal use of this product defects in products or 20 MACHEREY NAGEL 08 2012 Rev 02 PCR clean up components not manufactured by MACHEREY NAGEL or damages resulting from such non MACHEREY NAGEL components or products MACHEREY NAGEL makes no other warranty of any kind whatsoever and SPECIFICALLY DISCLAIMS AND EXCLUDES ALL OTHER WARRANTIES OF ANY KIND OR NATURE WHATSOEVER DIRECTLY OR INDIRECTLY EXPRESS OR IMPLIED INCLUDING WITHOUT LIMITATION AS TO THE SUITABILITY REPRODUCTIVITY DURABILITY FITNESS FOR A PARTICULAR PURPOSE OR USE MERCHANTABILITY CONDITION OR ANY OTHER MATTER WITH RESPECT TO MACHEREY NAGEL PRODUCTS In no
17. speed settings have to be adjusted carefully for each specific separation plate and shaker to prevent cross contamination from well to well Proceed as follows Adjusting shaker speed for binding and wash steps Load 300 uL dyed water select to the wells of the separation plate Place the plate on the shaker and start shaking with a moderate speed setting for 30 seconds Turn off the shaker and check the plate surface for small droplets of dyed water Increase speed setting shake for an additional 30 seconds and check the plate surface for droplets again Continue increasing the speed setting until you observe droplets on top of the separation plate Reduce speed setting check again and use this setting for the washing step Adjusting shaker speed for the elution step Load 100 uL dyed water to the wells of the collection plate and proceed as described above 2 5 Handling of beads Distribution of beads A homogeneous distribution of the magnetic beads to the individual wells of the separation plate is essential for a high well to well consistency Therefore before distributing the beads make sure that the beads are completely resuspended Shake the storage bottle well or place it on a vortexer shortly Premixing magnetic beads with the binding buffer allows easier homogenous distribution of the beads to the individual wells of the separation plate During automation a premix step before aspirating the beads binding buffer mixt
18. th elution buffer during the elution step The volume of dispensed elution buffer depends on the magnetic separation system e g the position of the pellet inside the separation plate For efficient elution the magnetic bead pellet should be resuspended completely in the elution buffer For some separators high elution volumes might be necessary to cover the whole pellet Elution is possible at room temperature Yield can be increased by 10 15 if elution is performed at 55 C 8 channel pipetting device 8 MACHEREY NAGEL 08 2012 Rev 02 PCR clean up 3 Storage conditions and preparation of working solutions Attention Buffers MP1 and MP2 contain chaotropic salt Wear gloves and goggles CAUTION Buffers MP1 and MP2 contain guanidine hydrochloride which can form highly reactive compounds when combined with bleach sodium hypochlorite DO NOT add bleach or acidic solutions directly to the sample preparation waste Storage conditions All components of the NucleoMag 96 PCR kit should be stored at room temperature 18 25 C and are stable for up to one year Before starting any NucleoMag 96 PCR protocol prepare the following Wash Buffer MP3 Add the indicated volume of ethanol 96 100 to Buffer MP3 Concentrate Mark the label of the bottle to indicate that ethanol was added Wash Buffer MP3 is stable at room temperature 18 25 C for at least one year NucleoMag 96 PCR 1x 96 preps 4x 96 prep
19. ure from the reservoir is recommended to keep the beads resuspended Magnetic separation time Attraction of the magnetic beads to the magnetic pins depends on the magnetic strength of the magnetic pins the selected separation plate distance of the separation plate from the magnetic pins and the volume to be processed The individual times for complete attraction of the beads to the magnetic pins should be checked and adjusted on each system It is recommended using the separation plates or tubes specified by the supplier of the magnetic separator Washing the beads Washing the beads can be achieved by shaking or mixing In contrast to mixing by pipetting up and down mixing by shaker or magnetic mixing allows simultaneous mixing of all samples This reduces the time and number of tips needed for the preparation MACHEREY NAGEL 08 2012 Rev 02 T PCR clean up Resuspension by pipetting up and down however is more efficient than mixing by a shaker or magnetic mix Method Resuspension Number of tips efficiency needed Magnetic mix Low Shaker Low Pipetting High acceptable good excellent 8 channel pipetting device 2 6 Elution procedures Purified DNA can be eluted directly with the supplied Elution Buffer MP4 or water pH 7 5 8 5 Elution can be carried out in a volume of gt 25 uL per 12 uL bead suspension lt is essential to cover the NucleoMag P Beads completely wi
20. warrant that all applications have been tested in MACHEREY NAGEL laboratories using MACHEREY NAGEL products MACHEREY NAGEL does not warrant the correctness of any of those applications Last updated 07 2010 Rev 03 Please contact MACHEREY NAGEL GmbH amp Co KG Tel 49 24 21 969 270 tech bio mn net com Trademarks KingFisher is a registered trademark of Thermo Fisher Scientific NucleoMag is a registered trademark of MACHEREY NAGEL GmbH amp Co KG Te MagS is a trademark of Tecan Group Ltd Switzerland All used names and denotations can be brands trademarks or registered labels of their respective owner also if they are not special denotation To mention products and brands is only a kind of information i e it does not offend against trademarks and brands and can not be seen as a kind of recommendation or assessment Regarding these products or services we can not grant any guarantees regarding selection efficiency or operation MACHEREY NAGEL 08 2012 Rev 02 21
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