Spectrophotometer Health & Safety Manual
Contents
1. 1 nm from 0 3 nm 0 5 nm 0 2nm 2nm Accuracy 240 nm to 330nm 0 002 A at 0 5 A 0 05 A lt 0 1 at 0 004Aat1A 0 5 or 0 003 A ae 0 005 Abs or 1 of the 2 0 or 0 010 A at 1 A using Absorbance 259 nm at 0 006 Aat 2 A within 0 5 of Absorbance to 3 000 A at 546 nm reading whichever is the to 1 000A at 546nm Neutral Accuracy 0 7 0 8 A at 440 nm 465 nm 546 1 nm l value to 3 000 A at 546 nm l i whichever is the larger greater at 546 nm whichever is the greater Density using uracil 590 nm 635 nm p Filters 0 005 A using 60 mg l K2Cr207 Full specification and operating instructions are contained within the relevant user manual 4 6 Manufacturing information Biochrom Ltd 22 Cambridge Science Name and address of manufacturer Park Milton Road Cambridge CB4 OFJ UK dB Technologies Cambridge Ltd FCC Name and ID notified body reg no 90528 Place and date of declaration NanoVue Plus Family Cambridge June 2010 GeneQuant Family Cambridge June 2010 Novaspec Family Cambridge June 2010 Ultrospec 10 2100 3100 5300 6300 Family Cambridge June 2010 Ultrospec 7000 8000 9000 Family Cambridge August 2011 Identity of person authorized to sign DoC Brian Clarkstone Technical Director Full specification and operating instructions are contained within the relevant user manual 29004778UM AA 09 2011 13 5 LEGAL GE imagination at work and GE Monogram are trademar2. Buckinghamshire HP7 9NA UK http www gelifesciences com imagination at work 29004778UM AA 09 2011
3. and is accessible in the lamp chamber Under normal use the lamp beam is confined within the instrument The unit should not be operated with the sample chamber lid open or the lamp housing lid removed as prolonged exposure to the beam may cause permanent eye damage Not applicable to Ultrospec 10 Novaspec III Plus 29004778UM AA 09 2011 4 A WARNING High voltages exist inside the Ultrospec 2100 3100 5300 6300 7000 8000 9000 GeneQuant 100 1300 and NanoVue Plus instruments Repair and maintenance should only be carried out by individuals trained specifically to work on these instruments A N CAUTION These instruments may be connected to a separate PC To preserve the integrity of the measuring equipment it is essential that the attached PC itself conforms to basic safety and EMC standards and is set up in accordance with the manufacturers instructions If in doubt consult the information that came with your PC In common with all computer operation the following safety precautions are advised e To reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings e To reduce the chance of cross contamination from biological samples use appropriate personnel protection measures and disinfectant wipes on keyboard and mouse AN CAUTION HOT SURFACE e These instruments may be fitted with heated cell holder accessories that depending on operat
4. internet at GE Healthcare Lifesciences home page www 1 gelifesciences com 1 2 Intended use Visible and UV Visible soectrophotometers shine light through a liquid sample and measure it s Absorbance This sample is typically held in a cuvette but when sample volume is limited an instrument such as the NanoVue Plus can be used where a 2 ul sample can be pipetted directly onto the measurement area Typical applications of spectrophotometers include DNA RNA Oligo concentration and purity measurements as well as protein determinations This manual addresses the needs of scientists and technicians who operate UV Visible spectrophotometers The level of information presented in this manual assumes the user possesses basic laboratory and technical skills and has the knowledge and documentation to safely operate If you need assistance with the instructions in this manual contact GE Healthcare for more information 1 3 Safety Notices These operating instructions contain Warnings and Cautions concerning the products with meanings as defined below A WARNING Warning indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood A N CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and cle
5. so that it is facing away from you Undo the black knurled screw on the lamp cover plate with your fingers and remove the plate Undo the large cross headed screw holding down the lamp assembly housing and tilt it upwards Slide the lamp plate out and unplug the cable connector e ifthe tungsten lamp has failed the replacement should be inserted onto the plate pushing it all the way down into its holder e lf the deuterium lamp has failed insert the tungsten lamp onto the plate as above and then replace the whole assembly with the new one Reconnect the cable connector and slide the lamp plate in until it locates Push the lamp assembly housing down and attach with the large cross headed screw Replace the lamp cover plate attach by re tightening the black knurled screw 10 Replace the lamp access cover and attach with the screw at the rear 11 Reconnect the power supply cord and switch the instrument on 12 Dispose of the faulty lamp in accordance with local regulatory practice After the lamp has warmed up sufficiently 30 minutes run a new baseline 5 8 Ultrospec 8000 9000 Lamp Change Switch the instrument off disconnect the power supply cord and allow lamps to cool Locate the lamp access cover at the rear of unit and remove the two top screws and slide top cover back and lift off Never operate the unit with the lamp housing cover removed il ti Remove the lamp and dispose of in accordance with loc
6. 64 V AC 50 60 Hz T 1 6 AH 250 V AC Anti Surge High breaking capacity Ultrospec 7000 8000 9000 units 85 264 V AC 50 60 Hz T 1 6 AH 250 V AC Anti Surge High breaking capacity NanoVue Plus units 100 240 V AC 50 60 Hz 3 A UK plug only e The instrument should be positioned so that the power cable may be readily removed in the event of a hazard or malfunction occurring e Site the instrument in an atmosphere free from dust and corrosive fumes e Ultrospec 2100 3100 5300 6300 instruments have the on off switch on the back of the instrument on the right hand side Ultrospec 7000 8000 9000 instruments have the on off switch on the left hand side of the instrument Ultrospec 10 Novaspec III Plus GeneQuant 100 1300 and NanoVue instruments have an on off button on the keypad to disconnect power from these units remove the power lead from the instrument The instruments will automatically perform some start up self diagnostic checks please wait for these to finish before attempting to use the equipment e The spectrophotometer may be fitted with a range of accessories for specific applications For the GeneQuant 100 1300 and NanoVue Plus these include a built in printer Bluetooth or SD card accessory The Ultrospec 2100 3100 5300 6300 7000 8000 9000 have a range of accessories that can be fitted in the sample compartment including a sipper long path length cell holder and thermostatted cell holder Full instructions on fitting acce
7. GE Healthcare Life Sciences Spectrophotometer Health amp Safety Document including General Operating Instructions Original Instructions Page finder 1 Health and Safety 1 1 Ly 1 3 1 4 LO to Scope Intended use Safety Notices General Safety General Hazards Unpacking and Installation 3 Operating and Maintenance Il 3 6 55 5 4 Da 3 6 i 30 Preparation before starting Performing a run cuvettes Performing a run NanoVue Plus Post run procedures User Maintenance Novaspec lIl Plus Lamp Change Ultrospec 5300 6300 Lamp Change Ultrospec 8000 9000 Lamp Change 4 Troubleshooting 4 1 4 2 4 5 4 4 4 5 4 6 Emergency Procedures Recycling Procedures Recycling of hazardous substances Disposal of electrical components Specifications Manufacturing information 5 Legal 29004778UM AA 09 2011 Mme BWW UN WG WO WO WO WO O NNN py O 14 12 12 12 12 15 15 14 1 HEALTH AND SAFETY 1 1 Scope This booklet covers the following ranges of instruments e Ultrospec 10 cell density meter e Novaspec Ill and Novaspec Plus Visible spectrophotometers e GeneQuant 100 amp 1300 UV Visible soectrophotometers e Ultrospec 2100 3100 5300 6300 7000 8000 9000 UV Visible spectrophotometers e NanoVue Plus micro volume spectrophotometer Translations of these instructions into 22 European languages are available on the User Manual CD supplied with the instrument and on the
8. Pias Changing Tungsten lamp removal of cell holder for cleaning GeneQuant 100 amp 1300 units Ultrospec 7000 unit Removal of cell holder for cleaning Ultrospec 2100 amp 3100 units Removal of cell changer for cleaning hanging D ium T Ultrospec 5300 amp 6300 units nein ing BUEN elie removal of cell changer for cleaning hanging D ium T Ultrospec 8000 amp 9000 units aae Peet Leng ieee removal of cell holders for cleaning E T E Cleaning of sample plates recalibration of pathlength 5 6 Novaspec III Plus Lamp Change Switch off the instrument remove the sample from the cell holder and disconnect the power supply cord Remove the protective layers at the lamp access and plug in points on the underneath of the instrument mN Remove the lamp wires from the groove by gently unclipping it Remove the lamp by twisting the lamp assembly counter clockwise Remove the lamp connection end by gently pulling with your fingers Replace with new lamp using the reverse of these actions 2 E E Dispose of the faulty lamp in accordance with local regulatory practice 5 7 Ultrospec 5300 6300 Lamp Change Switch off the instrument remove sample from cell holder and disconnect the power supply cord Allow time for lamps to cool 2 Locate the lamp access cover on the left hand rear side of the instrument remove the screw and push the cover back using the insert at its front 29004778UM AA 09 2011 9 9 Turn the lamp select mirror
9. al regulatory practice Follow the handling instructions supplied with the lamp Do not touch the glass envelopes of the replacement bulbs directly Deuterium lamp Tungsten lamp Replace the lamp cover and screw securely Attach power cord switch on and wait at least 30 minutes for the unit to warm up Perform a new instrument baseline on all bandwidth settings if variable bandwidth unit and save this as the permanent baseline see the online help or user manual Reset the lamp hours after replacing the lamp see the online help or user manual 29004778UM AA 09 2011 10 4 TROUBLESHOOTING Detailed in the table below are the most common faults found whilst using our spectrophotometers Instrument Problem Possible causes Ultrospec 10 A flashing Absorbance reading of 2 00 Abs is This indicates an Absorbance of more than obtained 1 99 and is therefore out of range The sample needs to be diluted Ultrospec 10 REF is displayed when T is pressed The instrument has not been zeroed before a sample measurement has been attempted All instruments A negative reading is obtained In normal measurements the test sample has a positive Absorbance compared to that of the Reference Negative readings will be obtained if the Reference and Test cuvettes are mixed up All instruments Unexpected results are obtained Any bubbles in solution will produce considerable error All instruments Absorbance values higher t
10. arly understood 29004778UM AA 09 2011 3 A WARNING e There are no bio hazardous materials within the unit however this unit could be used with bio hazardous samples Before using the instrument the customer should have in place decontamination procedures designed to protect laboratory workers from occupationally acquired infections The sample chamber cell holders are removable and may be decontaminated using the appropriate disinfectant for the bio hazard in question rinsed with distilled water and then allowed to dry The sample chamber and exterior may be wiped with a suitable disinfectant cleaning wipe It is the responsibility of the customer to ensure that the user of the equipment is provided with a safe working environment Any chemicals used in Analyses should be used stored and disposed of in accordance with manufacturer s guidelines and local safety regulations Toxic Fumes Efficient laboratory ventilation must be provided when working with volatile solvents or toxic substances Waste disposal Disposal of some solvents and chemicals may be classed as hazardous waste and must be dealt with in accordance with local regulatory practice e Personal protective equipment This is not required to operate the unit but the samples measured may require PPE A local risk assessment should be carried out e Decontamination Equipment returned for repair should include an appropriate decontamination certificate 1 4 General Sa
11. fety This equipment has been designed to conform to the following directives 2006 95 EC Low Voltage Equipment Safety Directive 2004 108 EC EMC directive 2002 96 EC Waste electrical and electronic equipment WEEE 2002 95 EC Restrictions of the use of certain Hazardous Substances in electrical and electronic equipment ROHS 2006 42 EC Machinery directive 1999 5 EC Radio and Telecommunications terminal equipment directive for Bluetooth devices Only for instruments with optional Bluetooth accessory Standards to which conformity is declared include EN61010 1 2001 Safety requirements for electrical equipment for measure ment control and laboratory use EN 61326 1 2006 Electromagnetic compatibility generic emission standard Electrical equipment for measurement control and laboratory use Classified as Class B for conducted and radiated emissions EN ISO 12100 2010 Safety of machinery Basic concepts general principles for design 1 5 General Hazards There a number of warning labels and symbols that may be present on your instrument These are there to inform you where a potential danger exists or particular caution is required Before commencing installation please take time to familiarise yourself with these symbols and their meaning This instrument is subject to the following hazards AN CAUTION Some of the above instruments contain a UV source which generates a light beam that traverses the sample chamber
12. han expected Possible issue with optical alignment Contact service support All instruments Absorbance values lower than expected Check sample cuvette filled to 20 mm from base of cuvette Possible stray light issue Contact service Support All cuvette models Instrument will not zero Cuvette placed in wrong orientation Wrong cuvette material used for wavelength Novaspec III Pixel clock too high Instrument needs Peet ferorcoeone smeo g opnar aiormeni conse serve suport Novaspec III Error code Flashing number Wavelength accuracy failure Instrument needs optical alignment Contact service Support initialisation screen instrument switched on initialisation screen reprogramming Contact service support GeneQuant 100 1300 Ultrospec Poor reproducibility on DNA concentration Wrong cuvette being used nota 15 mm 2100 3100 5300 6300 and purity measurements beam height or wrong material not letting Ultrospec 7000 8000 9000 UV light pass through Background correction mode not switched on Measured Absorbance too low should be gt 0 1 A for accurate results GeneQuant 100 1300 Ultrospec Check sample and reference beams are clear when instrument switched on Instrument fails start up calibration check 2100 3100 5300 6300 Ultrospec 7000 8000 9000 Ultrospec 5300 6300 check lamp life and replace if hours gt 1000 GeneQuant 100 1300 Ultrospec 2100 3100 7000 8000 9000 report failure message t
13. ion may become hot to touch Care should be taken to avoid touching the heated accessory when running at elevated temperatures 1 6 Unpacking and installation NanoVue Plus instrument External power supply e Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrument and report the problem to your supplier e Ensure your proposed installation site conforms to the environment conditions for safe operation e Indoor use 51O40 C e Maximum relative humidity 90 up to 31 C decreasing linearly to 50 at 40 C e Extremes of temperature may require recalibration of the unit for optimum performance e f the instrument has been stored in a cold environment then it should be allowed to come to thermal equilibrium for 2 to 3 hours before operation so that start up calibration is not compromised by condensation e The instrument must be placed on a stable level bench or table capable of taking its weight with sufficient space around the instrument for ventilation to circulate freely e The equipment must be connected to the local supply outlet using the provided power cables compatible voltages are shown in the table below Replace power inlet fuses only with the same type and rating as follows 29004778UM AA 09 2011 gt Ultrospec 2100 3100 units 85 264 V AC 50 60 Hz T 2 AH 250 V AC Anti Surge High breaking capacity Ultrospec 5300 6300 units 90 132 V or 180 2
14. ks of General Electric Company 2011 General Electric Company All rights reserved First published September 2011 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information http www gelifesciences com GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences AB Bjorkgaton 30 751 84 Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare Bio Sciences Corp 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 29004778UM AA 09 2011 14 29004778UM AA 09 2011 15 GE Healthcare offices GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan For contact information for your local office please visit www gelifesciences com contact GE Healthcare Limited Amersham Place Little Chalfont
15. o service support 29004778UM AA 09 2011 11 Ultrospec 10 Novaspec III Plus Instrument switches off after calibration You may be keeping you finger on the ON GeneQuant 100 1300 NanoVue OFF button too long so that the instrument Plus receives both ON and OFF signals and switches off after the calibration Try adjusting the timing of your finger press at switch on Novaspec plus Instrument fails to initialise cell holder Ensure sample plates are clean obstructed UV error message Novaspec plus Absorbance readings unstable Ensure Reference QA switched on Ensure Background correction is switched on Ensure 2 ul sample being used Novaspec plus Absorbance readings stable but incorrect Check pathlength calibration Detailed instructions on how to carry out the above user maintenance and trouble shooting for the different models may be found in the relevant user manual supplied ona CD with the instrument 4 1 Emergency Procedures In the event of a malfunction or hazard occurring the user responsible shall disconnect the unit from power and isolate the instrument for decontamination by an appropriate means if bio hazardous material is spilt on in or around the instrument Ultrospec 2100 3100 5300 6300 instruments have the on off switch on the back of the instrument on the right hand side Ultrospec 7000 8000 9000 instruments have the on off switch on the left hand side of the instrument Ultrospec 10 Nova
16. spec III Plus GeneQuant 100 1300 and NanoVue instruments have an on off button on the keypad to disconnect power from these units remove the power lead from the instrument 4 2 Recycling Procedures This equipment should be decontaminated before decommissioning and all local regulations shall be followed with regard to scrapping of the equipment 4 3 Recycling of hazardous substances Spectrophotometers contain hazardous substances Detailed information is available from your local GE Healthcare representative 4 4 Disposal of electrical components Waste of electrical and electronic equipment must not be disposed of as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment 29004778UM AA 09 2011 12 4 5 Specifications Specification NanoVue Plus Ultrospec Ultrospec Ultrospec Ultrospec Ultrospec Ultrospec Ultrospec GeneQuant GeneQuant Novaspec Novaspec Novaspec 9000 8000 7000 6300 5300 3100 2100 1300 100 Plus III 10 Wavelength 600 nm 200 1100 nm 190 1100 nm 190 1100 nm 190 900 nm 190 1100 nm 190 900 nm 330 800 nm Range dedicated Light Source Deuterium Tungsten Deuterium Tungsten Xenon Xenon 600 nm LED Ayolar fs be Absorbance Eo 3 Ato O t 125A 4Ato4A ps a ee Range A Eo A 0 5mm Pathlength 1mm to 100 mm 1mm to 100 mm a ee mm mm 2 nm across range Wavelength
17. ssories are within the instrument or accessory user manual 29004778UM AA 09 2011 6 5 OPERATING AND MAINTENANCE All spectrophotometers are operated in the same generic way by comparing the amount of light Absorbed by a sample when compared to a reference solution 3 1 Preparation before starting e The instrument is switched on and allowed to initialise Ultrospec 10 Novaspec III Plus GeneQuant 100 1300 and NanoVue Plus units have the on off switch on the keypad e Ultrospec 2100 3100 5300 6300 units have an on off switch on the back of the instrument on the right hand side e Ultrospec 7000 8000 9000 instruments have the on off switch on the left hand side of the instrument e The relevant software module for the application being carried out is selected from within the instrument software e g fixed wavelength measurement concentration measurement wavelength scan kinetics measurement or a dedicated method such as DNA concentration amp purity The options within the application set up the instrument parameters such as wavelength and define any calculations taking place on the measured results 5 2 Performing a run cuvettes On the Ultrospec 10 Novaspec III Plus GeneQuant 100 1300 and Ultrospec 2100 3100 5300 6300 7000 8000 9000 units samples are measured in a cuvette A suitable cuvette must be used for the application to be carried out it must be of the correct material to allow the selected wavelength of ligh
18. sults screen DNA application GeneQuant 100 3 3 Performing a run NanoVue Plus e On the NanoVue Plus units samples are measured on a sample plate Using a pipette a solution of reference material which is typically the solution that the sample is dissolved in is dropped onto the measurement position on the sample plate and the instrument zeroed by pressing the zero key on the keypad Pipette sample here 29004778UM AA 09 2011 8 e The reference solution is then wiped away with a tissue the sample is pipetted into place and measured by pressing the run button on the keypad e The measured results will be displayed on the instrument display 3 4 Post run procedures Cuvettes empty cuvette of sample and rinse with deionised water Cuvettes may be periodically cleaned by using commercially available cell cleaning solutions or dilute detergent solutions but should always be rinsed in deionised water NanoVue Plus wipe sample with tissue At end of sample run wipe plates with tissue wetting with deionised water The plates can also be cleaned with isopropyl alcohol or a dilute detergent solution but the plates should always be rinsed with deionised water after cleaning Sample disposal e Waste disposal Disposal of some solvents chemicals or samples may be classed as hazardous or bio hazardous waste and must be dealt with in accordance with local regulatory practice 5 5 User Maintenance Sievasoee ana Novacbec
19. t to pass through and must be of the correct physical size to fit the relevant instrument and the sample volume available The cuvette is filled with a solution of reference material which is typically the solution that the sample is dissolved in the cuvette is placed in the cell holder in the correct orientation as per the diagrams below Ultrospec 10 front to back All other units right to left e The instrument is then zeroed by pressing the Zero key on the keypad Ultrospec 10 Novaspec III Plus GeneQuant 100 1300 Ultrospec 2100 3100 Ultrospec 6300 29004778UM AA 09 2011 7 e The reference solution is then removed the cuvette filled with the sample and the sample measured by pressing the run button on the keypad The measured results will be displayed on the instrument display Ultrospec 10 Novaspec III Plus GeneQuant 100 1300 Ultrospec 2100 3100 Ultrospec 6300 e The Ultrospec 7000 8000 9000 instruments have a coloured Touchscreen for operation and a double beam optical system In normal operation a cuvette filled with reference solution is put in the rear cell holder a cuvette filled with sample in the front cell holder the relevant application method is loaded and the Run Icon pressed If a single cell is being used e g a microcell it should be filled with reference solution placed in the front cell holder the Zero icon pressed the cuvette filled with sample placed in the front cell holder and the Run Icon pressed e Typical re
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