Tissuelyser User Manual - UTA
Contents
1. F03 displayed Specified oscillation If the specified oscillation frequency is exceeded frequency exceeded the TissueLyser II switches itself off and F03 is displayed Contact QIAGEN Technical Services to request instrument repair The speed sensor may be defective F04 displayed Transparent hood not To protect the operator the drive motor of the lowered TissueLyser II will only start if the transparent hood has been lowered If the START key is pressed while the hood is raised F04 is displayed and the drive motor does not start If the hood is raised while the TissueLyser II is in operation the drive motor switches itself off and F04 is displayed To cancel the F04 display press the STOP key TissueLyser Il User Manual 04 2010 n r 33 Troubleshooting Guide Comments and suggestions F05 displayed Keys held down for too If one or more keys on the keypad are pressed long and held down for more than 15 seconds the drive motor of the TissueLyser Il switches itself off and F05 is displayed This is a safety mechanism to prevent unwanted changes in the operation of the TissueLyser Il should an object land on the keypad To cancel the FO5 display switch the TissueLyser Il off and then on again F06 displayed Speed sensor defective f one of the magnets controlling the speed of the TissueLyser ll is missing or defective the instrument switches itself off and2. FO6 is displayed Contact QIAGEN Technical Services to request instrument repair F07 displayed Solenoid switch If there is a defect in the cable for the solenoid defective switch which monitors the raising of the transparent hood the TissueLyser Il switches itself off and F07 is displayed Contact QIAGEN Technical Services to request instrument repair E 34 TissueLyser Il User Manual 04 2010 Appendix A Appendix A Technical data QIAGEN reserves the right to change specifications at any time Environmental conditions Operating conditions Power 100 240 V AC 50 60 Hz 150 W Mains supply voltage fluctuations are not to exceed 10 of nominal supply voltages Overvoltage Il category Air 5 40 C 41 104 F temperature Relative Maximum 80 humidity for temperatures up to 31 C linearly decreasing down to a relative humidity of 50 at 40 C Place of For indoor use only operation Altitude Up to 2000 m 6500 ft Pollution level 2 Transportation conditions Air 25 C to 60 C 13 F to 140 F in manufacturer s package temperature Relative Maximum of 75 noncondensing humidity The instrument can also be operated at 4 C under noncondensing conditions TissueLyser Il User Manual 04 2010 35 Appendix A Storage conditions Air 5 40 C 41 104 in manufacturer s package tem
3. or key causes the disruption time to increase or decrease rapidly without any pauses If 99 minutes is exceeded the disruption time starts from 10 seconds again TissueLyser Il User Manual 04 2010 27 Operating Procedures For the optimal disruption time for your application see the TissueLyser Handbook 7 Press the START key on the keypad The TissueLyser Il will now operate at the frequency specified in step 5 In addition the Time min sec display will count down the disruption time i e the display shows the time remaining If necessary the disruption time can be adjusted while the TissueLyser ll is in operation Note The TissueLyser II will not start if the transparent hood has not been lowered WARNING Moving parts A Do not leave the TissueLyser Il unattended during operation 8 When the remaining time on the Time min sec display reaches zero the TissueLyser Il stops operating The display resets to display the disruption time for the previous disruption procedure carried out To pause the TissueLyser II before the remaining time reaches zero press the STOP key on the keypad To continue operation of the TissueLyser Il from the time remaining press the START key To cancel operation of the TissueLyser Il press the STOP key twice The TissueLyser Il then goes into standby mode When the START key is pressed again the display is reactivated and the disruption time for the prev
4. placed in a 10 ml jar containing a grinding ball and shaken at high speed TissueLyser Adapter Sets Grinding Jar Sets and sample tubes can be precooled to minimize degradation of nucleic acids during sample disruption To find out which procedures require precooling see the TissueLyser Handbook TT TissueLyser Il User Manual 04 2010 13 General Description 3 2 Features of the TissueLyser II system TissueLyser Il The TissueLyser ll provides high speed shaking with oscillation frequencies of 3 30 Hz 180 1800 oscillations per minute Two clamps at the front of the instrument hold either a TissueLyser Adapter Set or a Grinding Jar Set Figures 1 and 2 A safety mechanism prevents the instrument from operating until the transparent hood is lowered over the TissueLyser Adapter Set or Grinding Jar Set Power is switched on and off at the power switch located at the back of the instrument Oscillation frequency can be adjusted in steps of 0 1 Hz using a keypad Figure 3 located on top of the instrument Disruption time from 10 seconds up to a maximum of 99 minutes can also be set using the keypad Figure 3 The disruption parameters for a particular application i e oscillation frequency and disruption time can be stored in the memory of the TissueLyser Il Up to 9 disruption parameters can be stored Figure 1 TissueLyser II closed The transparent hood is lowered and the clamps at the front of the instrume
5. replacement product is being supplied by QIAGEN provides free recycling of its WEEE marked electronic equipment in Europe If a replacement product is not being purchased from QIAGEN recycling can be provided upon request at additional cost To recycle electronic equipment contact your local QIAGEN sales office for the required return form Once the form is submitted you will be contacted by QIAGEN either to request follow up information for scheduling collection of the electronic waste or to provide you with an individual quote TissueLyser Il User Manual 04 2010 37 Appendix A EC declaration of conformity Name and address of the company QIAGEN GmbH QIAGEN Strasse 1 D 40724 Hilden Germany We herewith declare under our sole responsibility that the product TissueLyser II meets all applicable requirements of the following European Directives Low Voltage Directive LVD 2006 95 EC Electromagnetic Compatibility Directive EMC 2004 108 EC and the relevant harmonised standards IEC 61010 1 2001 IEC 61326 1 2005 Hombrechtikon 11 January 2010 Pit Muggli Director Business Excellence QIAGEN Instruments AG EE ns 38 TissueLyser Il User Manual 04 2010 Appendix B Appendix B Liability clause QIAGEN shall be released from all obligations under its warranty in the event repairs or modifications are made by persons other than its own personnel except in cases where the Company has given its written consent to
6. 293 422 911 Fax 01293 422 922 Technical 01293 422 999 106 e USA Orders 800 426 8157 Fax 800 718 2056 Technical 800 DNA PREP 800 362 7737 QIAGEN Sample amp Assay Technologies
7. 79 Canada Orders 800 572 9613 Fax 800 713 5951 Technical 800 DNA PREP 800 362 7737 China Orders 86 21 3865 3865 Fax 86 21 3865 3965 Technical 800 988 0325 Denmark Orders 80 885945 Fax 80 885944 Technical 80 885942 Finland Orders 0800 914416 Fax 0800 914415 Technical 0800 914413 France Orders 01 60 920 926 Fax 01 60 920 925 Technical 01 60 920 930 Offers 01 60 920 928 Germany Orders 02103 29 12000 Fax 02103 29 22000 Technical 02103 29 12400 Hong Kong Orders 800 933 965 Fax 800 930 439 Technical 800 930 425 Ireland Orders 1800 555 049 Fax 1800 555 048 Technical 1800 555 061 Italy Orders 800 789 544 Fax 02 334304 826 Technical 800 787980 Japan Telephone 03 6890 7300 Fax 03 5547 0818 Technical 03 6890 7300 Korea South Orders 080 000 7146 Fax 02 2626 5703 Technical 080 000 7145 Luxembourg Orders 8002 2076 Fax 8002 2073 Technical 8002 2067 Mexico Orders 01 800 7742 639 Fax 01 800 1122 330 Technical 01 800 7742 436 The Netherlands Orders 0800 0229592 Fax 0800 0229593 Technical 0800 0229602 Norway Orders 800 18859 Fax 800 18817 Technical 800 18712 Singapore Orders 1800 742 4362 Fax 65 6854 8184 Technical 1800 742 4368 Spain Orders 91 630 7050 Fax 91 630 5145 Technical 91 630 7050 Sweden Orders 020 790282 Fax 020 790582 Technical 020 798328 Switzerland Orders 055 254 22 11 Fax 055 254 22 13 Technical 055 254 22 12 e Q e e UK Orders 01
8. April 2010 TissueLyser Il User Manual For disruption of up to 48 or 192 biological samples QIAGEN Sample amp Assay Technologies QIAGEN Sample and Assay Technologies QIAGEN is the leading provider of innovative sample and assay technologies enabling the isolation and detection of contents of any biological sample Our advanced high quality products and services ensure success from sample to result QIAGEN sets standards in Purification of DNA RNA and proteins Nucleic acid and protein assays microRNA research and RNAi Automation of sample and assay technologies Our mission is to enable you to achieve outstanding success and breakthroughs For more information visit www giagen com Contents Contents 1 Safety Information 5 1 1 Proper use 2 1 2 Electrical safety 6 1 3 Environment 7 1 4 Biological safety 8 1 5 Chemicals 9 1 6 Waste disposal 9 1 7 Mechanical hazards 10 2 Introduction 11 2 1 About this user manual 11 2 2 Intended use of the TissueLyser Il 11 3 General Description 13 3 1 TissueLyser ll principle 13 3 2 Features of the TissueLyser ll system 14 3 3 Applications 20 4 Installation Procedures 21 4 1 Unpacking the TissueLyser Il 21 4 2 Installation requirements 21 4 3 Transporting and repacking the TissueLyser ll 23 4 4 Installation of a TissueLyser Adapter Set or Grinding Jar Set 24 4 5 Replacing the fuses 25 5 Operating Procedures 27 5 1 Sample disruption 27 5 2 Storing disruption
9. II see Appendix A page 35 TissueLyser Il User Manual 04 2010 9 Safety Information 1 7 Mechanical hazards WARNING Moving parts A Do not leave the TissueLyser II unattended during operation To ensure safety the TissueLyer II will not operate until the transparent hood is closed WARNING Moving parts A Damage or personal injury can occur if the TissueLyser Adapter Set or Grinding Jar Set is not securely attached Ensure that both adapters or grinding jars are of similar weight Do not operate the TissueLyser Il with only a single adapter or grinding jar installed 10 TissueLyser Il User Manual 04 2010 Introduction 2 Introduction Thank you for choosing the TissueLyser II system We are confident it will become an integral part of your laboratory Before using the TissueLyser Il it is essential you read this user manual carefully and pay particular attention to the safety information The instructions and safety information in the user manual must be followed to ensure safe operation of the TissueLyser Il and to maintain the TissueLyser Il in a safe condition 2 1 About this user manual This user manual provides information about the TissueLyser ll in the following sections Safety Information Introduction General Description Installation Procedures Operating Procedures Maintenance Procedures Eg ACE E Troubleshooting Guide Appendices The appendices contain th
10. ad mill designed for rapid and efficient disruption of up to 48 or 192 samples at the same time A wide variety of biological samples can be processed including human animal and plant tissues as well as yeast and bacteria Each sample is simultaneously disrupted and homogenized by high speed shaking with a bead in a sealed tube bead milling 3 1 TissueLyser Il principle Efficient sample disruption is a prerequisite for nucleic acid and protein purification procedures Incomplete sample disruption can lead to significantly reduced yields and can increase the risk of clogging when using purification columns or magnetic particles The TissueLyser Il thoroughly disrupts and simultaneously homogenizes biological samples in the presence of lysis buffer Plant tissues can alternatively be disrupted and homogenized in the absence of lysis buffer Tubes containing sample and bead are placed in a TissueLyser Adapter Set and shaken at a very high speed causing the samples to be disrupted and homogenized by the beating and grinding effect of the beads The tubes can be discarded after use which prevents cross contamination and provides time savings as there is no need to clean tubes after disrupting samples In addition there is no cross contamination during sample disruption as each tube is securely sealed with its own lid The TissueLyser II can also disrupt and homogenize large samples when used in combination with a Grinding Jar Set Samples are
11. adapter or grinding jar installed 4 5 Replacing the fuses The fuses in the TissueLyser II can be replaced as follows M Unplug the power cord connected to the TissueLyser II M The fuse compartment is located between the power switch and the power cord socket Figure 12 Press at the side of the fuse compartment to release it M Replace the fuses The following are required two MT 3 15 A fuses M Reinsert the fuse compartment and lock it into place TissueLyser Il User Manual 04 2010 25 Installation Procedures eee ae IIMA Figure 12 Backside of TissueLyser II Power switch Fuse compartment 8 Power cord socket 5 RS 232 port for service personnel 3 Safety information plate j Voltage rating plate j Safety information plate 26 TissueLyser Il User Manual 04 2010 Operating Procedures 5 Operating Procedures This section describes how to operate the TissueLyser Il system Before proceeding you should familiarize yourself with the features of the TissueLyser II Section 3 and have properly installed the instrument Section 4 5 1 Sample disruption The procedure below describes how to disrupt samples using the TissueLyser II For detailed information about sample disruption and protocols see
12. d press it lightly into the indentation in the clamp C gre LLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLILLOLLLLULLIZLIILIILLZ ZLLLLLLLLLLLLLALLLALALZLSLLLILLLLLNLunM 24 TissueLyser Il User Manual 04 2010 Installation Procedures M Turn the handwheel B clockwise with two fingers until the adapter or grinding jar is seated in the clamp and level and does not move freely Continue to rotate the handwheel clockwise until 6 8 easily audible clicks are heard the locking pin A rises and falls with each audible click The engaged locking pin prevents automatic opening of the adapter or grinding jar If the locking pin cannot be pulled upward to release it do not force it upward using a hammer or other tool as damage may occur To release the locking spin turn the handwheel slightly clockwise the locking pin should then be freely moveable M To remove the adapter or grinding jar lift the locking pin A and turn the handwheel B in the opposite direction CAUTION Damage to the instrument A Use of adapters and plasticware other than those described in this user manual may lead to damage to the clamps of the TissueLyser Il WARNING Moving parts A Damage or personal injury can occur if the TissueLyser Adapter Set or Grinding Jar Set is not securely attached Ensure that both adapters or grinding jars are of similar weight Do not operate the TissueLyser Il with only a single
13. e carried out at room temperature or after freezing the grinding jars in liquid nitrogen For more information see the product sheet supplied with the Grinding Jar Set Figure 6 Grinding Jar Set S Steel Each 10 ml jar is made of stainless steel and contains a 20 mm diameter stainless steel grinding ball Grinding Jar Set Teflon The Grinding Jar Set Teflon cat no 69986 allows disruption of 2 large samples in parallel using Teflon grinding balls Figure 7 Sample disruption can be carried out at room temperature For more information see the product sheet supplied with the Grinding Jar Set Z Figure 7 Grinding Jar Set Teflon Each 10 ml jar is made of Teflon and contains a 20 mm diameter Teflon grinding ball 18 TissueLyser Il User Manual 04 2010 General Description TissueLyser Single Bead Dispensers TissueLyser Single Bead Dispensers dispense individual beads into any sample container Figure 8 They are available in 2 sizes M Tissuelyser Single Bead Dispenser 5 mm cat no 69965 Dispenses beads of 5 mm diameter and contains a reservoir that can hold approximately 150 beads M Tissuelyser Single Bead Dispenser 7 mm cat no 69967 Dispenses beads of 7 mm diameter and contains a reservoir that can hold approximately 45 beads The dispensers can be cleaned with water or detergent For more information see the product sheet supplied with the TissueLyser Si
14. e clamps of the TissueLyser ll CAUTION Damage to the instrument A Avoid spilling water or chemicals onto the TissueLyser Il Damage caused by water or chemical spillage will void your warranty In case of emergency switch off the TissueLyser Il at the power switch and unplug the power cord from the power outlet 1 2 Electrical safety WARNING Electrical hazard A Any interruption of the protective conductor earth ground lead inside or outside the TissueLyser Il or disconnection of the protective conductor terminal is likely to make the TissueLyser ll dangerous Intentional interruption is prohibited Lethal voltages inside the TissueLyser ll When the TissueLyser ll is connected to line power terminals may be live and opening the TissueLyser Il or removing parts is likely to expose live parts To ensure satisfactory and safe operation of the TissueLyser Il follow the advice below MB The line power cord must be connected to a line power outlet that has a protective conductor earth ground M Do not adjust or replace internal parts of the TissueLyser Il M Do not operate the TissueLyser II with any covers or parts removed M If liquid has spilled inside the TissueLyser II switch off the TissueLyser II disconnect it from the power outlet and contact QIAGEN Technical Services 6 TissueLyser Il User Manual 04 2010 Safety Information M If the TissueLyser II becomes elect
15. e following M Technical data E Warranty terms 2 2 Intended use of the TissueLyser Il The TissueLyser Il is intended for molecular biology applications This product is neither intended for the diagnosis prevention or treatment of a disease nor has it been validated for such use either alone or in combination with other products Therefore the performance characteristics of the product for clinical use i e diagnostic prognostic therapeutic or blood banking are unknown The TissueLyser Il is not designed for continuous operation but for single 8 hour shifts with 30 operating time QIAGEN has successfully tested the TissueLyser Il with the following adapter sets and grinding beads M TissuelLyser Adapter Set 2 x 96 with one 3 mm diameter tungsten carbide bead or one 5 mm diameter stainless steel bead per collection microtube M TissuelLyser Adapter Set 2 x 24 with one 3 mm diameter tungsten carbide bead or one 5 mm or 7 mm diameter stainless steel bead per microcentrifuge tube TissueLyser Il User Manual 04 2010 11 Introduction Damage to the clamps of the TissueLyser II The clamps of the TissueLyser II are designed for use with the following QIAGEN adapters and consumables M TissueLyser Adapter Set 2 x 24 cat no 69982 containing standard 2 ml microcentrifuge tubes M TissueLyser Adapter Set 2 x 96 cat no 69984 containing Collection Microtubes racked cat no 19560 sealed with Collection Microtube Cap
16. els on the TissueLyser II Risk of personal injury and material damage Only perform maintenance that is specifically described in this user manual Servicing The TissueLyser II is supplied with a warranty that lasts for 12 months starting from the date of shipment The warranty includes all repairs due to mechanical breakdown 6 1 Regular maintenance The TissueLyser ll should be wiped after use using a soft cloth moistened with a suitable cleaning agent see below To remove residual cleaning agent and avoid instrument damage the TissueLyser II should then be immediately wiped with a soft cloth moistened with water Be sure to switch off the TissueLyser Il at the power switch and unplug the power cord from the power socket before cleaning The components of the TissueLyser Adapter Set or Grinding Jar Set should be cleaned after use using a suitable cleaning agent see below rinsed with distilled water and then dried using paper towels If the TissueLyser II is contaminated with infectious material it should be decontaminated The TissueLyser Il should also be decontaminated before shipping and a decontamination certificate must be completed to certify that the instrument has been decontaminated To request a decontamination certificate contact QIAGEN Technical Services 30 TissueLyser Il User Manual 04 2010 Maintenance Procedures Cleaning agents The following disinfectants and detergents are
17. f the TissueLyser II and avoids invalidation of your guarantee 4 2 Installation requirements Removal of the transport security device A transport security device is located on the underside of the TissueLyser II this is comprised of 2 screws see Figure 10 Using a screwdriver remove the 2 screws Do not discard the screws as they will need to be reinstalled if the TissueLyser I needs to be transported in the future oo N TissueLyser Il User Manual 04 2010 21 Installation Procedures Transport security device Figure 10 Transport security device The transport security device comprised of 2 screws indicated by the arrows is located on the underside of the TissueLyser II CAUTION Damage to instrument A Operation of the TissueLyser II with the transport security device installed or transport of the TissueLyser II without the transport security device installed can lead to damage to the mechanical components of the instrument Site requirements Place the TissueLyser Il on a flat stable surface and ensure that there is sufficient space around and under the instrument for ventilation The TissueLyser Il is for indoor use only under the following conditions M Ambient temperature within the range of 5 40 C 41 104 F M Up to 2000 m 6500 ft above mean sea level MSL M Maximum relative humidity of 80 for temperatures up to 31 C linearly decreasing down to a relative humidity of 50 a
18. ious procedure is displayed switch When the TissueLyser ll is no longer in operation switch it off at the power 5 2 Storing disruption parameters The TissueLyser ll has an internal memory which has 9 storage locations to store disruption parameters i e disruption time and oscillation frequency To store disruption parameters follow the procedure below 1 Switch on the TissueLyser Il on appears in the Memory display Press the PROG key repeatedly until the desired storage location in the memory is displayed i e P1 to P9 Press the SET key This causes all displays to flash Set the disruption time and oscillation frequency using the and keys Note To cancel the operation press the PROG key The disruption parameters selected will not be stored Press the SET key again The display stops flashing and the disruption 2 3 4 on the keypad 5 parameters are stored 28 TissueLyser Il User Manual 04 2010 Operating Procedures To retrieve disruption parameters from the memory press the PROG key repeatedly until the desired storage location i e P1 to P9 is displayed Then press the START key to start operation of the TissueLyser Il 5 3 Displaying the accumulated operating time To display the total amount of time that the TissueLyser Il has been operated follow the procedure below 1 When the TissueLyser ll is in standby mode sim
19. ive bacteria For information about specific applications and protocols for sample disruption see the TissueLyser Handbook 20 TissueLyser Il User Manual 04 2010 Installation Procedures 4 Installation Procedures 4 1 Unpacking the TissueLyser II Before unpacking the TissueLyser II check whether the cardboard container is damaged In case of damage contact the transporter of the container The TissueLyser Il must not be knocked shaken or thrown during transport otherwise its electronic and mechanical components may be damaged Make a note of how the TissueLyser ll is packed If returning the instrument to QIAGEN for repair it needs to be repacked using the original packaging When lifting the TissueLyser II place both hands below the base of the instrument After unpacking the TissueLyser Il check that the following items are supplied M Tissuelyser II M Power cords for different countries M Tissuelyser I User Manual MB TissuelLyser Handbook If anything is missing contact QIAGEN Technical Services Check that the TissueLyser II is not damaged If anything is damaged contact QIAGEN Technical Services Make sure that the TissueLyser II has equilibrated to ambient temperature before operating it The TissueLyser Il should be stored dry when not in use Retain the original packaging in case you need to return the TissueLyser II to QIAGEN for repair Using the original packaging minimizes damage during transportation o
20. l is intended to supplement not supersede the normal safety requirements prevailing in the user s country 1 1 Proper use WARNING Risk of personal injury and material damage A Improper use of the TissueLyser II may cause personal injuries or damage to the instrument Do not attempt to move the TissueLyser ll during operation The TissueLyser Il should only be operated by qualified personnel who have been appropriately trained Servicing of the TissueLyser II should only be performed by QIAGEN or an authorized agent service technicians Do not carry out any modifications of the instrument QIAGEN shall not be held responsible for any injuries or damages resulting from nonobservance of the instructions and safety information in this user manual The TissueLyser II should only be used for the applications described in the TissueLyser Handbook The TissueLyser Il should only be used in combination aay TissueLyser Il User Manual 04 2010 5 Safety Information with accessories approved by QIAGEN see Section 3 2 page 14 Do not continue to use the TissueLyser ll or TissueLyser accessory if it is damaged Perform the maintenance as described in Section 6 QIAGEN charges for repairs that are required due to incorrect maintenance CAUTION Damage to the instrument A Use of adapters and plasticware other than those described in this user manual may lead to damage to th
21. ld be used only for the TissueLyser Adapter Sets and Grinding Jar Sets M If solvents or saline acidic or alkaline solutions are spilt on the TissueLyser Il wipe the spilt liquid away immediately M Follow manufacturer s safety instructions for handling cleaning agents W Follow manufacturer s instructions for soaking time and concentration of cleaning agents exposure for longer than the recommended soaking time can damage the TissueLyser II TissueLyser Adapter Sets and Grinding Jar Sets TissueLyser Il User Manual 04 2010 31 Maintenance Procedures WARNING A Toxic fumes Do not use bleach to clean or disinfect used labware Bleach in contact with salts from the buffers used can produce toxic fumes A CAUTION Damage to the instrument A Do not use bleach solvents or reagents containing acids alkalis or abrasives to clean the TissueLyser Il CAUTION Damage to the instrument Avoid spilling water or chemicals onto the TissueLyser Il Damage caused by water or chemical spillage will void your warranty 6 2 Regular safety checks Every 6 months the limit switch and the brake should be checked for correct operation To check the limit switch and the brake M Remove the TissueLyser Adapter Set or Grinding Jar Set from the clamps of the TissueLyser II Switch on the TissueLyser II Set the oscillation frequency to 30 Hz Press the START key Raise the transpare
22. ngle Bead Dispenser Figure 8 TissueLyser Single Bead Dispenser The dispenser dispenses individual beads into any sample container TissueLyser Bead Dispensers 96 Well TissueLyser Bead Dispensers 96 Well dispense 96 beads in parallel into Collection Microtubes racked Figure 9 They are available in 2 sizes M TissueLyser 3 mm Bead Dispenser 96 Well cat no 69973 Dispenses beads of 3 mm diameter and contains a reservoir that can hold approximately 1000 beads M Tissuelyser 5 mm Bead Dispenser 96 Well cat no 69975 Dispenses beads of 5 mm diameter and contains a reservoir that can hold approximately 300 beads The dispensers can be cleaned with water or detergent For more information see the product sheet supplied with the TissueLyser Bead Dispenser 96 Well TissueLyser Il User Manual 04 2010 19 General Description OE LL SSS 2 2 DU gt 2 a a G Figure 9 TissueLyser Bead Dispenser 96 Well The dispenser dispenses 96 beads into a rack of collection microtubes Beads The following beads for the TissueLyser II are available from QIAGEN M Stainless Steel Beads 5 mm cat no 69989 M Stainless Steel Beads 7 mm cat no 69990 M Tungsten Carbide Beads 3 mm cat no 69997 3 3 Applications The TissueLyser ll enables disruption and homogenization of Human and animal tissues Human and animal cells Plant tissues Yeast Gram positive and gram negat
23. nt are empty Note The maximum oscillation frequency may drop down to 25 Hz depending on local fluctuations in the mains voltage 14 TissueLyser Il User Manual 04 2010 General Description Set is installed Figure 2 TissueLyser II open The transparent hood is raised and a TissueLyser Adapter auuu aa TissueLyser Il User Manual 04 2010 15 General Description Figure 3 Keypad of the TissueLyser II A Memory display with PROG and SET keys for storing disruption parameters 3 Frequency 1 s display with and keys for adjusting oscillation frequency Time min sec display with and keys for adjusting disruption time 5 START and STOP keys for starting and stopping operation of the TissueLyser Il TissueLyser Adapter Set 2 x 24 The TissueLyser Adapter Set 2 x 24 cat no 69982 allows disruption of 48 samples in parallel using standard 2 ml microcentrifuge tubes e g Eppendorf Safe Lock micro test tubes The adapter set contains 2 adapters with each adapter comprised of a top plate a bottom plate and a tube holder for holding 24 microcentrifuge tubes Figure 4 Sample disruption can be carried out at room temperature or after storing the adapter set at 80 C for at least 2 hours The adapter set can be cleaned with detergent microbicides or up to 96 ethanol For more information see the product sheet supplied with the adapte
24. nt hood The drive motor should switch itself off and F04 should appear in the display M Lower the transparent hood The drive motor should not switch itself on again If there is a defect contact QIAGEN Technical Services to request repair of the instrument TissueLyser Adapter Sets and Grinding Jar Sets may become worn depending on the frequency of use and the samples disrupted This may lead to damage to the clamps of the TissueLyser II Check the TissueLyser Adapter Sets and Grinding Jar Sets regularly for wear and tear and replace if necessary 32 TissueLyser Il User Manual 04 2010 Troubleshooting Guide 7 Troubleshooting Guide 7 1 Error codes displayed by the TissueLyser II Comments and suggestions FO1 displayed Drive motor of When the drive motor is overloaded the TissueLyser II TissueLyser II switches itself off to allow the drive overloaded motor to cool A display on the TissueLyser Il alternates between F01 and the remaining cooling time in minutes e g 03 indicates 3 minutes After the cooling time has elapsed switch the TissueLyser II off and then on again before continuing to operate the instrument F02 displayed Drive motor of If the drive motor does not start after the START TissueLyser ll not key has been pressed contact QIAGEN starting Technical Services to request instrument repair The drive motor may be blocked or the motor o speed sensor may be defective
25. pair please contact QIAGEN to request a Declaration of Decontamination form After completing and signing this form place it in an envelope which should then be attached to the external surface of the cardboard container In addition place a copy of the form inside the container To repack the TissueLyser Il first reinstall the transport security device using the two original screws supplied with the instrument for details see Section 4 2 Then return the TissueLyser II to its original packaging by following the unpacking steps in reverse order ME TissueLyser Il User Manual 04 2010 23 Installation Procedures 4 4 Installation of a TissueLyser Adapter Set or Grinding Jar Set The TissueLyser ll is not supplied with any accessories TissueLyser Adapter Sets and Grinding Jar Sets need to be ordered separately to order the TissueLyser Il or its accessories visit www giagen com Products TissueLyserll aspx Figure 11 Left hand clamp of the TissueLyser II 1 Locking pin H Handwheel Indentation in clamp Attach a TissueLyser Adapter Set or Grinding Jar Set to the TissueLyser Il as follows M Pull the locking pin A upward out of its slot and rotate it by 90 Figure 11 This releases the locking device MB Turn the handwheel B counterclockwise until the maximum clamping range is reached M Rotate back the locking pin A by 90 until it engages with its slot M Insert the adapter or grinding jar an
26. parameters 28 5 3 Displaying the accumulated operating time 29 5 4 Displaying the software version 29 6 Maintenance Procedures 30 6 1 Regular maintenance 30 6 2 Regular safety checks 32 7 Troubleshooting Guide 33 7 1 Error codes displayed by the TissueLyser II 33 TissueLyser Il User Manual 04 2010 3 Contents Appendix A 35 Technical data 35 Environmental conditions 35 Waste Electrical and Electronic Equipment WEEE 37 EC declaration of conformity 38 Appendix B 39 Liability clause 39 4 TissueLyser Il User Manual 04 2010 Safety Information 1 Safety Information Before using the TissueLyser ll system it is essential that you read this user manual carefully and pay particular attention to the safety information The instructions and safety information in the user manual must be followed to ensure safe operation of the TissueLyser Il and to maintain the TissueLyser ll in a safe condition The following types of safety information appear throughout this user manual WARNING The term WARNING is used to inform you about situations A that could result in personal injury to you or other persons Details about these circumstances are given in a box like this one CAUTION The term CAUTION is used to inform you about situations that A could result in damage to the TissueLyser II or other equipment Details about these circumstances are given in a box like this one The advice given in this manua
27. perature Relative Maximum of 85 noncondensing humidity Mechanical data Dimensions Height 266 mm up to 524 mm with the hood raised Width 371 mm Depth 461 mm Mass Approx 26 kg without TissueLyser Adapter Set or Grinding Jar Set installed Oscillation 3 30 Hz frequency Noise The noise characteristic values are also influenced by the characteristic properties of the sample to be disrupted values Sound power level Lwa 71 4 dB A Emission value related to workplace LA 61 dB A 36 TissueLyser Il User Manual 04 2010 Appendix A Waste Electrical and Electronic Equipment WEEE This section provides information about disposal of waste electrical and electronic equipment by users in the European Union The European Directive 2002 96 EC on WEEE requires proper disposal of electrical and electronic equipment when it reaches its end of life The crossed out wheeled bin symbol see below indicates that this product must not be disposed of with other waste it must be taken to an approved treatment facility or to a designated collection point for recycling according to local legislation The separate collection and recycling of waste electronic equipment at the time of disposal helps to conserve natural resources and ensures that the product is recycled in a manner that protects human health and the environment QIAGEN accepts its responsibility in accordance with the specific WEEE recycling requirements and where a
28. perform such repairs or modifications All materials replaced under this warranty will be warranted only for the duration of the original warranty period and in no case beyond the original expiration date of original warranty unless authorized in writing by an officer of the Company Read out devices interfacing devices and associated software will be warranted only for the period offered by the original manufacturer of these products Representations and warranties made by any person including representatives of QIAGEN which are inconsistent or in conflict with the conditions in this warranty shall not be binding upon the Company unless produced in writing and approved by an officer of QIAGEN TissueLyser Il User Manual 04 2010 39 Notes 40 TissueLyser Il User Manual 04 2010 Notes TissueLyser Il User Manual 04 2010 41 Notes 42 TissueLyser Il User Manual 04 2010 Trademarks QIAGEN QIAGEN Group DNA ExitusPlus AppliChem Gigasept Lysetol Mikrozid Sch lke amp Mayr GmbH DECON QUAT Veltek Associates Inc Teflon E du Pont de Nemours and Company 2009 2010 QIAGEN all rights reserved eee www qiagen com Australia Orders 1 800 243 800 Fax 03 9840 9888 Technical 1 800 243 066 Austria Orders 0800 28 10 10 Fax 0800 28 10 19 Technical 0800 28 10 11 Belgium Orders 0800 79612 Fax 0800 79611 Technical 0800 79556 Brazil Orders 0800 557779 Fax 55 11 5079 4001 Technical 0800 5577
29. r set Use of other tubes in combination with 7 mm diameter beads may result in the beads getting lodged in the lids of the tubes 16 TissueLyser Il User Manual 04 2010 General Description Figure 4 TissueLyser Adapter Set 2 x 24 Each adapter consists of a top plate a bottom plate and a tube holder for holding 24 microcentrifuge tubes 2 ml TissueLyser Adapter Set 2 x 96 The TissueLyser Adapter Set 2 x 96 cat no 69984 allows disruption of 192 samples in parallel using Collection Microtubes racked and Collection Microtube Caps cat nos 19560 and 19566 The adapter set contains 2 adapters with each adapter comprised of a top plate and a bottom plate for holding a rack of capped collection microtubes Figure 5 Sample disruption can be carried out at room temperature or after storing the adapter set at 80 C for at least 2 hours The adapter set can be cleaned with detergent microbicides or up to 96 ethanol For more information see the product sheet supplied with the adapter set Figure 5 TissueLyser Adapter Set 2 x 96 Each adapter consists of a top plate and a bottom plate for holding a rack of capped collection microtubes TissueLyser Il User Manual 04 2010 17 Grinding Jar Set S Steel General Description The Grinding Jar Set S Steel cat no 69985 allows disruption of 2 large samples in parallel using stainless steel grinding balls Figure 6 Sample disruption can b
30. recommended for cleaning the TissueLyser Il TissueLyser Adapter Sets and Grinding Jar Sets Note If you want to use disinfectants different from those recommended ensure that their compositions are similar to those described below General cleaning MB Mild detergents B 70 ethanol Disinfection M Ethanol based disinfectants can be used for disinfection of surfaces e g 25 g ethanol and 35 g 1 propanol per 100 g liquid or Mikrozid Liquid Schilke amp Mayr GmbH cat no 109160 M Disinfectants based on glyoxal and quaternary ammonium salt can be used for submerging the TissueLyser Adapter Sets and Grinding Jar Sets e g 10 g glyoxal 12 g lauryldimethylbenzylammonium chloride 12 g myristyldimethylbenzylammonium chloride and 5 15 nonionic detergent per 100 g liquid Lysetol AF Gigasept Instru AF in Europe cat no 107410 or DECON QUAT 100 Veltek Associates Inc in the USA cat no DQ100 06 167 01 Removal of RNase contamination M RNosekKiller 5 PRIME cat no 2500080 can be used for cleaning surfaces and spraying the TissueLyser Adapter Sets and Grinding Jar Sets Removal of nucleic acid contamination M DNA ExitusPlus AppliChem cat no A7089 0100 can be used for cleaning surfaces and submerging the TissueLyser Adapter Sets and Grinding Jar Sets General instructions M Do not use spray bottles to spray cleaning or disinfectant liquids onto surfaces of the TissueLyser II Spray bottles shou
31. rically unsafe prevent other personnel from operating it and contact QIAGEN Technical Services the TissueLyser Il may be electrically unsafe when Mit or the line power cord appears to be damaged M It has been stored under unfavorable conditions for a prolonged period Mt has been subjected to severe transport stresses If the TissueLyser ll is not in use switch it off at the power switch If the TissueLyser II will not be used for a prolonged period of time unplug the power cord from the power outlet 1 3 Environment Operating conditions WARNING Explosive atmosphere A The TissueLyser Il is not designed for use in an explosive atmosphere WARNING Risk of explosion A The TissueLyser ll is intended for use with reagents and substances supplied with QIAGEN kits Use of other reagents and substances may lead to fire or explosion CAUTION Damage to the instrument A Direct sunlight may bleach parts of the instrument and cause damage to plastic parts The TissueLyser ll must be located out of direct sunlight EE TissueLyser Il User Manual 04 2010 7 Safety Information 1 4 Biological safety Specimens and reagents containing materials from humans should be treated as potentially infectious Use safe laboratory procedures as outlined in publications such as Biosafety in Microbiological and Biomedical Laboratories HHS www cdc gov od ohs biosfty biosfty htm Samples Samples may con
32. s cat no 19566 QIAGEN adapters together with the recommended tubes form a component that is of an optimal thickness allowing secure tightening of the clamps of the TissueLyser II without straining the clamping mechanism Damage to the clamps of the TissueLyser II will occur if other components of reduced thickness are installed Therefore the TissueLyser Il must not be used with adapters from other suppliers or with QIAGEN adapters containing tubes not recommended by QIAGEN Requirements for TissueLyser II users This table covers the general level of competence and training necessary for transportation installation use maintenance and servicing of the TissueLyser ll Training and Task Personnel experience Transportation Installation routine use and maintenance Servicing No special requirements Laboratory technicians or equivalent QIAGEN or an authorized agent service technicians No special requirements Appropriately trained and experienced personnel Use of the TissueLyser Il with non QIAGEN adapters has been tested by other suppliers e g Roche recommend use of the TissueLyser II with GS FLX Titanium emPCR Shaker Adapters LV which are accessories for the Genome Sequencer FLX Instrument Use of such adapters has not been tested by QIAGEN 12 TissueLyser Il User Manual 04 2010 General Description 3 General Description The TissueLyser II is a flexible be
33. sruption process Always wear safety glasses gloves and a lab coat The responsible body e g laboratory manager must take the necessary precautions to ensure that the surrounding workplace is safe and that TissueLyser ll operators are not exposed to hazardous levels of toxic substances chemical or biological as defined in the applicable Material Safety Data Sheets MSDSs or OSHA ACGIH or COSHH documents Venting for fumes and disposal of wastes must be in accordance with all national state and local health and safety regulations and laws OSHA Occupational Safety and Health Administration United States of America t ACGIH American Conference of Government Industrial Hygienists United States of America COSHH Control of Substances Hazardous to Health United Kingdom Toxic fumes If working with volatile solvents toxic substances etc you must provide an efficient laboratory ventilation system to remove vapors that may be produced WARNING Toxic fumes A Do not use bleach to clean or disinfect used labware Bleach in contact with salts from the buffers used can produce toxic fumes 1 6 Waste disposal Used labware such as sample tubes may contain hazardous chemicals or infectious agents from the sample disruption process Such wastes must be collected and disposed of properly according to local safety regulations For information on how to dispose of the TissueLyser
34. t 40 C 22 TissueLyser Il User Manual 04 2010 Installation Procedures CAUTION Damage to instrument A Operation of the TissueLyser II at a temperature and humidity outside the range specified may lead to damage to its electronic and mechanical components as well as a reduction in instrument performance Power requirements The power line to the TissueLyser II should be voltage regulated and surge protected Make sure that the voltage rating of the TissueLyser II see the plate on the back of the instrument is compatible with the AC voltage available at the installation site Grounding requirements To protect operating personnel the National Electrical Manufacturers Association NEMA recommends that the TissueLyser Il be correctly grounded earthed The instrument should be plugged into an AC power outlet that has a ground earth connection 4 3 Transporting and repacking the TissueLyser II Carry the TissueLyser Il with both hands below the base of the instrument Please retain all original packaging for the duration of the warranty period and beyond A charge may be incurred in the event of damage during shipment if the original packaging is not used for the return of the TissueLyser II to QIAGEN for repair CAUTION Damage to instrument A Transport of the TissueLyser II using packaging other than the original packaging may lead to instrument damage Before returning the TissueLyser Il for re
35. tain infectious agents You should be aware of the health hazard presented by such agents and should use store and dispose of such samples according to the required safety regulations WARNING A Samples containing infectious agents Some samples used with the TissueLyser ll may contain infectious agents Handle such samples with the greatest of care and in accordance with the required safety regulations Always wear safety glasses 2 pairs of gloves and a lab coat The responsible body e g laboratory manager must take the necessary precautions to ensure that the surrounding workplace is safe and that TissueLyser ll operators are suitably trained and not exposed to hazardous levels of infectious agents as defined in the applicable Material Safety Data Sheets MSDSs or OSHA ACGIH or COSHH documents Venting for fumes and disposal of wastes must be in accordance with all national state and local health and safety regulations and laws OSHA Occupational Safety and Health Administration United States of America t ACGIH American Conference of Government Industrial Hygienists United States of America COSHH Control of Substances Hazardous to Health United Kingdom TissueLyser Il User Manual 04 2010 Safety Information 1 5 Chemicals WARNING Hazardous chemicals A Some chemicals used with the TissueLyser II may be hazardous or may become hazardous after completion of the sample di
36. the TissueLyser Handbook 1 Make sure that the TissueLyser Adapter Set or Grinding Jar Set is securely installed on the clamps of the TissueLyser II see Section 4 4 page 24 and that the transparent hood is lowered WARNING Moving parts A Damage or personal injury can occur if the TissueLyser Adapter Set or Grinding Jar Set is not securely attached Ensure that both adapters or grinding jars are of similar weight Do not operate the TissueLyser Il with only a single adapter or grinding jar installed P9 Make sure that the TissueLyser II is connected to a power outlet ES Switch on the TissueLyser Il using the power switch 4 Check that on appears in the Memory display If necessary press the PROG key repeatedly until on is displayed 5 Using the or key under the Frequency 1 s display set the oscillation frequency Briefly pressing the or key changes the oscillation frequency by 0 1 Hz Continuous pressing of the or key causes the oscillation frequency to increase or decrease rapidly without any pauses The selectable range of frequencies is 3 30 Hz For the optimal oscillation frequency for your application see the TissueLyser Handbook 6 Using the or key under the Time min sec display set the disruption time Briefly pressing the or key changes the disruption time by 1 second Continuous pressing of the
37. ultaneously press the SET key and the key under the Frequency 1 s display 2 bS appears in the Memory display In addition the accumulated operating time for the TissueLyser II is displayed in hours and minutes in the Frequency 1 s and Time min sec displays 3 Press the STOP key to cancel the display 5 4 Displaying the software version To display the software version installed on the TissueLyser Il follow the procedure below 1 When the TissueLyser ll is in standby mode simultaneously press the SET key and the key under the Frequency 1 s display 2 S appears in the Memory display In addition the software version is displayed in the Frequency 1 s display 3 Press the STOP key to cancel the display TissueLyser Il User Manual 04 2010 29 6 Maintenance Procedures Perform the following regular maintenance procedures i e after each daily use to ensure reliable operation of the TissueLyser Il Maintenance Procedures CAUTION A Damage to the instrument Do not use bleach solvents or reagents containing acids alkalis or abrasives to clean the TissueLyser Il If solvents or saline acidic or alkaline solutions are spilt on the TissueLyser Il wipe them away immediately Do not autoclave any part of the TissueLyser Il TissueLyser Adapter Set or Grinding Jar Set WARNING A Risk of electric shock Do not open any pan
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