Release Notes
Contents
1. 9 9 Rename a Sequence Name If you want to change the name that you have given to a Sequence for example from LAS Montage you can select the sequence in the Navigation tree and click the Rename button or press F2 You can now type in a new name 9 10 Rename and delete and archive Under Setup gt Archive the Management panel only available to Administrators now allows you to rename a non active Archive If you wish to delete an archive as an administrator you need to select the archive and enter your user name and tick the Confirm Deletion check box LAS Release Notes Page 23 Delete Archive x Archive Name LAS Mechanical User Name Confirm Deletion The Archive folders are moved to the Recycle bin 9 11 Edition shown in caption You can easily see the Edition of LAS that is being used because it is shown to the left of the menu bar LAS Archive Standard File Options Help EE a ee 9 12 LAS Core archive creation restrictions You cannot create or use Folder Archives with LAS Core 9 13 Example report templates A number of report templates are installed To use these with the DM microscopes create an archive using the template called LAS Project Template as shown Source New e From Template CADocuments and Settings All Users Documents Leica Application Suite Archive Templates LAS Project Templateat AR With this Archive you can use the 3 templates ject mjas Project 22. Demo camera can load jpegs The Gamma curve is displayed on the histogram panel 9 22 LAS MultiFocus and Montage The algorithm used to create the result image has been improved and is now less sensitive to out of focus image noise The images produced show significantly fewer artefacts than previously Images created using optics that shift the image due to the change of focus during acquisition are also sharper due to improved image alignment techniques LAS Release Notes Page 27 10 Web Sharing in LAS 10 1 Introduction Web Sharing enables an LAS user to make the camera live image available to one or more remote users across a network or the internet The remote user can view the LAS live image by connecting to a web page via a Web Browser and must have access to the internet and or access to the same network as the LAS user Web Sharing comes in two software parts one for the LAS user the server and the other for the remote user the client The server software is installed if it is selected in the LAS installation list of additional modules The client software is the user s Web Browser but reguires the use of an additional browser component called Microsoft Silverlight Currently Internet Explorer and Mozilla FireFox Web Browsers are supported although others also work but some may not 10 2 Licence Web Sharing requires an LAS licence to work It is visible in LAS and can only be used if you have a valid demo or ful
3. changing microscope to None The problem is solved if the user restarts LAS after changing configuration Annotation Merge This facility is not available for 16 or 48 bit images On a Greyscale image the annotation is converted to greyscale when it is merged To avoid this firstly convert the image to 24 bit colour from the Process gt Enhance tab LAS Release Notes Page 12 LAS appears disabled when Twain is minimised If the Twain window is opened from LAS and minimised and another Windows program become active the main LAS window will appear when selected from the Windows taskbar However this is disabled because Twain is open To activate Twain use Alt Tab and not the taskbar LAS operation after LAS EZ If both LAS and LAS EZ are installed and LAS EZ is run the next time LAS is run it will not start completely Please restart the PC and LAS will now start We do not expect customers to be using both products on a PC so this is only of concern for demo use 6 2 Restrictions with LAS Modules LAS Image Analysis For images with a resolution higher than 2592 x 2048 such as from Leica DFC490 DFC500 the following advanced features are not available Selected feature highlight Colour coded images Feature Outline Magnifier Edit accept reject keep delete For images gt 4096 x 4096 IM will be disabled LAS Interactive Measurements The Export to Word facility requires that Microsoft Word is fully licensed
4. 1 Introduction 1 2 Reason for this Release Version V3 3 0 1 3 System Requirements 3 3 1 Supported Hardware cien ate tae EA EA EEN 3 3 2 Computer System Hegutrements EEN 3 3 3 Computer Operation erger Eeer EE rnu mm me 3 34 Other Leica Sortwa re eebe aain e EEEE dE ale 4 4 Release History 5 4 1 Version V3 3 0 release reinoirei easa and aa aaia aa eaters 5 42 Version V373Drelegeg ENEE 5 4 3 Version V3 1 0 releaS vach ua tae teal te tana Ane tee a EE hy 5 44 Version V3 0 1 E 6 45 Version V3 0 0 release mmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmr iHHi arm mm 6 5 Problems Fixed 9 5 1 Problems fixed In Meier tate ede data 9 5 2 Problems fixed E 9 5 3 Problems fixed i VI O a a aa aaa a a a e a a a a e aa a aaie iaa men 9 5 4 Problems fixed m V3 0 0 ENEE 9 6 Restrictions 10 6 1 et 10 6 2 Restrictions with LAS Module 13 6 3 Restrictions with LAS Apple atons ENEE 15 6 4 COMPETE aein a EEN 15 6 5 IR EEN 15 6 6 lee Del 16 7 Installation and Licensing 17 1 1 ristalling LAS eelere 17 72 Installing LAS Steel Expert ENEE 17 73 Licensing Optional Module ENEE 17 74 License Transfer e ee ert asasi aai Ee ee AEN 18 7 5 User Interface Language Selection in LASEL 18 8 Software Version Numbers Included on this CD 20 9 Notes concerning the use of new features 21 9 1 Demo Camera to Simulate a live Imane EE 21 9 2 Image saving format OPTIONS EE 21 9 3 Image export format options EE 22 9 4 Decimal places in Preferences EEN 22 9 5 Changing units and decimal p
5. LCD CLS 150XD CLS 150LS or EL6000 one will also get the control panels at start up of LAS even without power ON of the unit In this case you can switch ON the power and continue your work without restarting LAS KL2500 and EL6000 are not equipped with electronics that allow active feedback while pressing the test button in the hardware setup panel Please make sure the light source is connected to the right COM port and test the function using the LAS main window If you get corrupted or wrong filter names at start up with MZ 16FA please press lt Refresh gt to update External illumination KL 2500 LCD CLS 150 XD CLS 150 LS EL6000 is not supported in the store and recall module and memory positions Stage XYZ positions are not recalled by Store and Recall The storage of positions within the live image is not possible with the DMI inverted microscopes and Stereomicroscopes It works fine with the uprights LAS Release Notes Page 16 7 Installation and Licensing The software to control the microscope and Leica DFC cameras can be used in accordance with the software license You do NOT need to apply for a license key if this is your intended use The remaining optional modules can be used for 60 days evaluation and must be licensed for extended use The procedure for this is detailed in section 7 3 7 1 Installing LAS The installation of the Leica Application Suite LAS is described in the document LAS Install Guide in the
6. and download the firmware It is possible to select the XY and Z drive to be controlled by Oasis Blue with a DM6000 This means that other LAS modules such as LAS Montage can be operated without needing to reconfigure the focus drive for the DM6000 You will see that in the Acquire gt PM gt Optics Settings gt Initialise and Set Speed dialog under the Autofocus tab there is a new button labelled Calibrate This performs a calibration of the Autofocus and is required to ensure that the backlash of the focus drive is measured and appropriate compensated applied Before using Autofocus you should find a field of detail on the specimen that can be sharply focussed and click Calibrate The focus control will move to measure the backlash and save this value for the objective used The histogram upper and lower limits are now applied to the PM images provided the histogram and exposure panels on the camera tab are not floating 9 20 Universal Objectives Additional universal objectives may now be used such as x80 please check the Setup Microscope for details To change the C mount right click in the list and enter a new value 9 21 LAS Camera Software Update You can delete shading references in LAS Saved camera configurations and shading are now camera serial number specific LAS Release Notes Page 26 Demo camera is automatically installed with DFC cameras but not DFC500 There is no option in the installer
7. attached to record and replayed 12730207 LAS Archive Standard LAS Release Notes Page 7 Allows multi level database structure in a hierarchy of levels Allows new database structure to be created Text Memo Numeric Boolean Date Keyword fields Keyword list creation and use Any data from selected records can be output to a Word template Example Word templates are provided and can be customized by the user Output via Word template directly to pdf html and Word Example multi level databases provided For details of earlier versions of LAS please see the Release Notes included with the earlier versions LAS Release Notes Page 8 5 Problems Fixed 5 1 Problems fixed in V3 3 0 Montage Compound Weighted method is corrected 5 2 Problems fixed in V3 2 0 The white balance no longer fails unexpectedly Search using Help no longer causes problems A magnification changer not in the camera light path no longer changes calibration Scalebar now appears in Web Sharing Client on German 0 S Filters can be manually adjusted while using DMI4000 with LAS Image Overlay without errors 16bit grey or Colour images are now acquired in Image Overlay b3 Problems fixed in V3 1 0 The magnification changer can be set to none in the Setup gt Mic select magnification changer and right click in the table select delete When dual camera ports are used the calibration now updates to the selected port for appropriate microscopes Search
8. now returns records from all levels 5 4 Problems fixed in V3 0 0 This is the first release of this version of LAS Please refer to the section on limitation on use for know issues Problems fixed in versions of LAS prior to this release can be found in the release notes of these versions LAS Release Notes Page 9 6 Restrictions 6 1 Software Core Windows version This software is not supported on the following environments Macintosh Unix Linux Windows Vista Starter Home Windows XP other than Professional Edition Windows 2000 any version Windows 98 Windows NT Windows 95 64 bit Windows XP Personally constructed PCs or operating systems An environment that is an upgrade of the original manufacture installed operating system Multi boot environments Language Translations The user interface is translated into the usual languages Multilingual Windows is strongly recommended particularly for Chinese and Japanese Native Windows for Chinese Japanese and Korean do not display the user interface correctly The help file and user manual are in English or in some case refer to the earlier V3 2 version of LAS Please contact DI Support for updates if your language does not appear LAS Steel Expert user manual is not translated Leica Server Version Note that the Leica Server version compatible with LAS V3 3 0 is V3 10 0 If you are using Leica QWin MW FW or CW software and have any doubts about compatibility p
9. 84f3658a5 amp DisplayLang en Select the correct language and download the patch file You CANNOT install the patch properly just by double clicking on it You need to run it from a DOS prompt or use the Run option from the Start menu The command you use will look like this WindowsXP KB904706 v2 x86 ENU exe b SP2QFE The file name is specific for each language the one above is English ENU so make sure you use the filename and path for the file you have Use b SP2QFE as the command line parameter option otherwise LAS won t work properly although the patch will install LAS Image Overlay Does not use the external filter wheel Does not control the lamp on an external light source In the unlikely event that image corruption is noticed with full frame high resolution images e g from Leica DFC490 it is probably that the PC memory has been exceeded Please reduce the image resolution and try again LAS Power Mosaic In Turboscan mode occasionally a white frame may be observed If this occurs please check that the hard drive has ample free space and is defragmented regularly If this continues to occur it indicates that the performance of the PC is not sufficient and a faster processor and a faster hard drive are required Calibration of exported mosaics is not correct on binned images and DFC290 XGA and VGA modes These modes are not recommended with Power Mosaic as they reduce the resolution of the mosaic countering the object
10. AS licence to another PC please contact LASConfCent leica microsystems com 7 5 User Interface Language Selection in LAS The user interface language of LAS may be changed by System Administrators An example of the use of this could be when an English reader wishes to use LAS on a Windows Japanese systems A utility program is provided for this purpose called LanguageSetter exe This program is installed in the folder C Program Files Leica Microsystems Leica Application Suite V3 Ensure LAS is closed before running the LanguageSetter Double clicking on this program will show the single dialog as below LAS Release Notes Page 18 Select the language required and click OK Appropriate fonts must be available for Chinese and Japanese languages A restart will be required Note that this does not change the language of Leica DFC Twain which can only be changed by re installing the software LAS Release Notes Page 19 8 Software Version Numbers Included on this CD The following software versions are provided on this CD Leica LAS V 3 3 0 Leica DFC Camera V 6 12 1 LAS Release Notes Page 20 9 Notes concerning the use of new features The following notes describe in brief the use of features not included in the Help 9 1 Demo Camera to simulate a live image This is a feature of DC Twain V6 12 that allows a DFC camera to be simulated This is useful for training when a real camera is not available T
11. C500 cannot be used with this feature Hot swapping Hot swapping of cameras while LAS is running is not supported Please close LAS before changing cameras It is recommended that LAS is closed LAS before swapping cameras Hot swapping while Leica DFC Twain window is open from LAS can cause LAS to hang If this occurs use Task Manager to kill the framework exe process so that LAS can be used again System suspend We do not ensure trouble free operation of the system suspend sleep or hibernation functions on all computers in conjunction with LAS Matrox Meteor 2 Leutron Frame Grabbers Not supported 6 6 Microscopes We recommend the use of RS232 COM1 for firmware download for DMIs All other serial interfaces as well as the USB interface may also be used but occasionally the download stops and has to be restarted DM microscopes are not affected Firmware updates may be required before LAS will communicate correctly with some microscopes The serial connection to the Stereo and Macroscopes MUST be connected to the MotorFocus MST 31 if available If you are using a Z6APOA or Z16APOA in combination with MotorFocus MST31 you MUST connect the serial connection to the zoom If you are using an IsoPro stage additionally you MUST connect to the USB of the stage Note that the initialisation dialog may disappear behind the splash screen at start up Please use lt ALT gt gt TAB gt to bring it in front again If using KL 2500
12. G is a bitmapped image format that employs lossless data compression PNG was created to improve upon and replace the GIF format as an image file format When the Jpeg option is selected the level of compression can be selected as shown LAS Release Notes Page 21 In this format Jpeg b With this quality jpeg only Medium Compression _ Best Quality High Quality Medium Compr High Compression Best quality corresponds to the compression used in previous versions of LAS The other options use more compression and sacrifice image quality High compression is very blocky and useful only for emailing 9 3 Image export format options The image formats for exporting include Photoshop 3 in addition to the image saving formats Note that if you export an image with a format different from the format with which it is saved in the archive it will take longer to export because it has to be loaded and converted Image type Change image type Tiff Is PNG Bitmap Photoshop 3 The Jpeg option allows the quality to be selected as for image saving 9 4 Decimal places in Preferences The number of decimal places shown when measurements are displayed is set in Preferences Measurements Display 4 H Decimal places The number of decimal places is stored for each image as an archive field when the image is acquired 9 5 Changing units and decimal places If you wish to change the number of decimal pl
13. Leica Application Suite Release Notes V3 3 0 Seica MICROSYSTEMS Leica Application Suite Release Notes V 3 3 0 Abstract This document describes the V3 3 0 release of the Leica Application Suite You should read this document before installing your copy of this software This document also acts as a supplement to the User Guide All reasonable steps have been taken to ensure that this publication is correct and complete but should any user be in doubt about any detail clarification may be sought from Leica Microsystems Switzerland Ltd or their accredited representative The information in this document is subject to change without notice and should not be construed as a commitment by Leica Microsystems Switzerland Ltd Leica Microsystems Switzerland Ltd accepts no responsibility for any errors that may appear in this document Copyright 2008 Leica Microsystems Switzerland Ltd All rights reserved The contents of this publication may not be reproduced in any form or communicated to a third party without prior written permission of Leica Microsystems Switzerland Ltd Due to a policy of continuous development we reserve the right to change specifications without notice Microsoft and MS DOS are registered trademarks and Windows the Windows logo the Windows 2000 logo the Windows XP logo and the Windows Vista logo are trademarks of Microsoft Corporation Date Nov 2008 Issue 4 Printed in UK Table o Contents
14. aces or the Units used after acquisition such as when measurements are displayed this can be done for single images by the panel on the Process gt Calib tab Decimal Places Units ER Note that the values shown on the measurement display do not change until the measurement is drawn existing measurements are not changed LAS Release Notes Page 22 9 6 Confirming image name on acquisition When an image is acquired its name is constructed from the Default Image Name and an incremental number as specified in the Preferences gt Image tab However if you set the check mark Always Confirm Image Name when the image is acquired a prompt will be displayed and you can enter a new image name for each image The default name appears in the prompt but you can start typing straight away and the default will be removed The name you enter will be the name of the image file on the hard drive If the name already exists the incremental number will be appended The name you type will become the Default Image Name From this dialog you can also select a new folder location 9 7 Adjusting the Gallery thumbnail size The size of the thumbnail images in the gallery can be adjusted by the slider to the right of the gallery The smallest size of thumbnails are shown below 9 8 Vertical Scalebar The Scale Bar orientation can be selected Scale Bar o Show Orientation Horizontal H Horisontal Pixel Lenath Vertical
15. ample database is provided for general use User can add image records and sequence records Can be used with all optional modules User can make a new database from the supplied templates User cannot add or rename fields Data limit of 4Gb no limit of images in database Used with all LAS supported microscopes and cameras Form view is provided and an image note and description can be added Individual images can be printed with heading and footer Export images with a user defined file name Is used ona single PC Compared to LAS 2 8 enhancements include Gallery is shown on acquire Gallery loading speed much improved Acquire directly from Browse Large image view can also show gallery The data view of all workflow steps can be individually customised Demo of non licensed modules can continue after licensing of some modules New licensing method used any V2 x options will need to be re licensed Hardware dongle Part No 12730205 is optionally available for licensing 12730206 LAS Archive Basic User can add and name categories User can add text fields to database User can rename fields in database Shows grid view and form view Allows selection of fields for display on data form Displays data from all LAS supported microscopes and cameras Search for text in all fields of records and search for text in specified fields only Allows multiple files to be attached to record such as a word document Allows Sound file to be recorded
16. arType option is switched on Please check that this is the case by looking in Start gt Control Panel gt Display gt Appearance gt Effects as shown below Effects LG E Use the following transition effect for menus and tooltips Fade effect 2 Use the following method to smooth edges of screen fonts ClearType C use large icons C Show shadows under menus Show window contents while dragging Hide underlined letters for keyboard navigation until I press the Alt key Exit and restart When you close the LAS application but do not exit to Windows it takes a few seconds for the microscope to be closed Please wait a few seconds before restarting LAS Alternatively exit directly to Windows Autofocus Module The following cameras only are supported Leica DFC280 320 480 300FX 350FX Store and Recall Recall for focus drive and x y position is not available for specimen security Image Overlay and use of 16 bit images is not supported with Store and Recall Font shown on buttons If the text shown on the dialog buttons does not appear to fit correctly you may have selected the wrong screen fonts in the display driver The fonts used must be the small fonts shown at 96dpi Often larger fonts are selected when high resolution displays are used but these do not scale correctly to the buttons sizes Changes in configuration LAS is susceptible to changes in configuration e g change of camera
17. e Version V3 3 0 The reasons for this release are e The introduction of LAS Image Explorer Folders for image acquisition and browsing in LAS Core Compatible with all LAS Modules e The introduction of a new archiving capability based on Windows Folders The 2 optional modules are LAS Archive Basic and LAS Archive Standard Further details are given below Compatible with all LAS Modules e Removal of previous SQL based archiving that is replaced by LAS Folder Archive Basic and LAS Folder Archive Standard e Introduction of LAS Image Analysis module for the thresholding and automatic measurement of images Further details are given below e Update to LAS Steel Expert LAS Image Acquisition and Management LAS operates in 2 distinct modes depending how the user wishes to manage the acquired images These are known as LAS Image Explorer And LAS Folder Archive LAS Image Explorer is used when images are stored to the hard drive in an informal manner The user can save the images anywhere on the hard drive in any folder and organizes the images as convenient While this method has the benefit of simplicity there is a chance that over time the images will become harder to find LAS Folder Archives are used where a more disciplined approach to the organization of data is demanded This might occur where it is mandatory to save additional user defined fields with the images or where several users need to add data to images in a systemat
18. er than the display resolution This means that fixed Reticules can be shown on any live image rather than having to be modified to suit any the specific image size 9 17 Diameter display with Manual Measurement For the display of a circle the parameter displayed can be selected from Area Radius or Diameter from the Select Parameters panel under Analysis gt Setup This feature only applies to the Circle tool LAS Release Notes Page 25 Select Parameters ebe Circle Tool D a Area Radius F Diameter 9 18 Measurement data file linked to record automatically When measurements are saved you now have the option of linking the Excel sheet produced directly to the record Report Append to Excel worksheet Create Report Create Report and Attach Select the report output to be Append to Excel worksheet and the click Create Report and Attach When you look to the Browse gt Browse gt Attachments panel you will see the Excel sheet you have created appears and can be opened immediately This save the time taken in manually attaching the Excel sheet to the record Reminder Microsoft Office 2000 or earlier is not supported LAS does not generate any results with this version of Office 9 19 Power Mosaic Please note that is essential to check that the Oasis Blue board has the latest firmware V0 64 installed The Oasis drivers and firmware are on the LAS DVD and these should be used to check
19. etup b Starting the Hardware Setup executable directly Alternatively create a short cut to the Hardware Setup application located at C Program Files Leica Microsystems Leica Application Suite V3 Libraries HWModel Hardware configuration exe For non standard installations consult your IT administrator to find out where LAS was installed 34 Other Leica Software You must use Leica Servers V3 10 0 with this version of LAS in conjunction with Leica QWin or MW LAS Release Notes Page 4 4 Release History 4 1 Version V3 3 0 release This release see section 2 42 Version V3 2 0 release The reasons for this release are Stereo and Macroscopes Support for the following is added M205A motorized high end stereomicroscope M50 and M80 manual routine stereomicroscopes LED5000 CXI coaxial LED illumination LED3000 NVI near vertical LED illumination Firmware update for Z6APOA and Z16APOA for compatibility with new MotorFocus Leica DFC Cameras Support for the following new cameras is added e Leica DFC295 using 1394b interface e Leica DFC310 FX using 1394b interface e Leica ICC50 camera module for DM500 amp DM750 DM Microscopes Support for the following is added e Leica DM500 e Leica DM750 LAS Core Software LAS is now normally supplied on a single DVD The organisation of the DVD is such that it can be copied onto 5 CDs and each CD installed individually if necessary LAS is supplied
20. for Windows Vista 32 and 64 bit and Windows XP 32 bit Some hardware is not compatible with 64 bit operation Please see the section restrictions LAS Imaging Modules LAS MultiFocus and Montage Enhancements to extended focus algorithms improve performance with difficult images 4 3 Version V3 1 0 release The reasons for this release are Stereo and Macroscopes Support for the following is added e M205FA fully motorized fluorescence stereomicroscope with 20 5 1 zoom range e M165FC manual fluorescence stereomicroscope with coded filter wheel zoom and iris and 16 5 1 zoom range e M125 manual stereomicroscope with 12 5 1 zoom range e New motorized focus drive MST51 e New FluoCombi attachment for fluorescence stereomicroscope with coded objective positions e New Fluorescence objective revolver with coded objective positions Leica DFC Cameras Support for the following new cameras is added LAS Release Notes Page 5 e Leica DFC290 HD e Leica DFC400 DM Microscopes Support for the following is added e FSC Version Il is now fully supported e Firmware for STP6000 for FSC LAS New Software Modules The following new modules are introduced e LAS Live Measurements see Help file for description e LAS Web Sharing see section 10 of this for operating notes e LAS Reticule now integrated in LAS see Help file for description LAS Core Software Enhancements to LAS Core include further detai
21. for the module s 2 You will receive a license number for the registration of these modules each license might cover or one or more modules 3 Fill out the information in the registration form accessed via LAS and send the form to the configuration centre LASConfCent leica microsystems com 4 Receive a license file from the configuration centre and select this from the LAS License dialog You will need have Administrator privileges to license LAS LAS Release Notes Page 17 The licensing procedure is described in the document LAS Install Guide in the root folder of the LAS DVD You should read this document before installing your copy After installation the document can be accessed from the Start Menu gt Leica Application Suite You can examine your registered modules from the menu Registration Information Your purchase also gives you access to the Leica Application Suite e mail Hotline in case of technical questions di support leica microsystems com Or MQM Hotline leica microsystems com When using the Hotline please mention the exact configuration you are working with and your software version release You will find this information by selecting the About command from the Help menu This will facilitate the analysis of your query and accelerate our response time Product improvement suggestions and comments are also welcome and can be sent to the Hotline 74 License Transfer If you wish to move the L
22. he Demo Camera is installed at the same time as DFC Twain but not with DFC500 Twain The Demo Camera is selected from the Hardware Setup Image Source Please select the camera framegrabber you want to use as image source SCH Leica Demo Camera None ao Leica DFC Cameras Leica DFC500 Leica Demo Camera When you access Acquire gt Camera the image shown is simply one that is recalled from the hard drive it is not a real live image of course Consequently the controls do not have any real function they are simply present for simulation However an image can be acquired just like a real live image The image that is displayed can be replaced by another image from your hard drive if you don t find the example appropriate If you open the Twain interface you can see that there is an entry Select image If you right click on this you can browse to find the image you wish to use It must be a BMP or JPG image and it must be larger than 1024 x 768 24 bit If the image is not suitable you will see flashing coloured squares Select image C Documents ane Captured Image 9 2 Image saving format options The image save format is selected from the Preferences dialog and now includes Jpeg2000 and PNG In this format Joea Cd Tiff Jpeg Jpeg2000 J2K Jpeg2000 is a wavelet based image compression standard that can give less blocky images with similar file size to normal Jpeg Portable Network Graphics PN
23. he PC is overloaded too full or being used for too many tasks it may become sluggish in operation Please check the following points regularly LAS performance may be limited if there is less than 400 Mbyte of free RAM because other running programs may occupy RAM Other running programs may occupy the processor and these may limit LAS s performance It is recommended to close other programs Virus scanners AntiSpyware programs and similar may affect the performance of LAS If this is the case consult your system administrator to reduce checks on LAS components Check the hard drive has 50 free space If the space is used up consider installing a larger drive The hard drive must be defragmented regularly Please select High quality or Optimal quality where Direct 3D options are offered by the graphics card and not balanced which appears by default The location of the archive is linked to the LAS user who must have write access to this folder Store data to a local drive and not to a mapped network location as this can be very slow due to the network speed The archive images and metadata can be stored on a remote drive if the speed is acceptable Note that a back up device and a back up strategy for archives images and data are essential The user must ensure that regular and reliable back up of important files is performed User Accounts User log On and Off is by use of Windows accounts so that the com
24. ic manner This also means that images are saved in locations where other users will be able to find them In this case it is an advantage to restrict the locations that the images are saved We call these Folder Archives because the images are still saved to the Windows file system but only in predefined locations LAS is designed to be used on a single PC and does not provide multi user network access Data and images can be saved to any mapped network drive where the performance will depend on the bandwidth available The image management capabilities of LAS are expressed by different optional Editions with increasing capability namely LAS Core LAS Basic and LAS Standard The features of these are LAS Core using Image Explorer LAS Release Notes Page 1 Features the storages of images and metadata in Windows folders Controls and displays data from all LAS supported microscopes and cameras Used with all LAS supported microscopes and cameras Can be used with all optional modules Form view is provided and an image note and description can be added Individual images can be printed with heading and footer Export images with a user defined file name 12730206 LAS Folder Archive Basic Databases with one sub level plus the image level Can be used with all optional modules User can add image records and seguence records User can make a new database from the supplied templates User can add and name categories User can add
25. l licence The following panel will be displayed in the Acquire gt Camera tab Web Sharing ox Image size 640 x 480 GB Enable 10 3 Starting Web Sharing in LAS To start Web Sharing check Enable The size of the live image a remote user will see is made by selecting the Image Size The default size is 640x480 and nominally produces about 10 fps for the remote user depending on the live image exposure However the frame rate ultimately is determined by the flow of network traffic and the internet connection for the LAS user and remote user Other options are 800x600 and 1024x768 but the larger the size the slower the frame rate will be for the remote user Enabling Web Sharing will slow the performance of the LAS computer slightly However the more remote users are connected the LAS computer performance will become slower Web Sharing requires that a DFC or demo camera should be connected Otherwise an error dialog will be displayed Web Sharin Web sharing cannot be started when no camera is connected Once Web Sharing is enabled the panel will also display the network address of the LAS computer the URL Web Sharing disables other LAS functions not related to the camera LAS Release Notes Page 28 Web Sharing v Ka Image size Enable http 193 128 84 150 Las Here the address is shown as http 193 128 84 150 Las and it is this address which the remote user will need to c
26. laces ENEE 22 9 6 Confirming image name ON aCgulsitlon EE 23 9 7 Adjusting the Gallery thumbnail size NENNEN 23 9 8 MOM Cal S Cale Dat Zaire seg Aere EE 23 9 9 Rename a Sequence Name ENEE 23 9 10 Rename and delete and archive EE 23 9 11 Edition SHOWN in Caption EE 24 9 12 LAS Core archive creation restrictons EE 24 9 13 Example report Template ENEE 24 9 14 Licensing and Hegtsttaton EE 25 9 15 License Tlename 24i ci geg 25 9 16 Reticule uses fit tO WINdOW EE 25 9 17 Diameter display with Manual Measurement EE 25 9 18 Measurement data file linked to record automatically ceccsessessesessessesesseeteeseseesteeeeseeeees 26 9 19 AANE AAY O EET LOA AE E A AAEE REA E A E E A T abet ee 26 9 20 Universal OH S il fa nanena a eegen dE 26 9 21 LAS Camera Software Update 26 9 22 LAS MultiFocus and Montage ENEE 27 10 Web Sharing in LAS 28 10 1 10 2 10 3 10 4 10 5 IMEFOUU ele 28 PUG ue 28 Starting Web Sharing in LAS ENEE 28 RemotE USET neneiia n AA R EE 29 Dim tatl Hee EES 30 1 Introduction This document describes the release of the Leica Application Suite LAS You should read this document before installing your copy LAS is supplied on a single DVD If you have just received a new Leica computer system the software will have already been installed and configured and you should ignore the sections on Installation and Configuration but please take the time to read the remaining sections 2 Reason for this Releas
27. lease contact DI Support on di support Leica Microsystems com for advice concerning the correct versions of software to install Please do not assume compatibility Installation The installation procedure assumes that all the software components are installed on the first hard disk drive i e C During the installation of LAS you can select the destination directory and drive for LAS however this release does not support the installation of LAS on drives other than C LAS V3 2 does not have to be uninstalled before installing LAS V3 3 but this is recommended After installing LAS V3 3 it is not possible to revert to LAS V3 2 Before installing LAS V3 3 we recommend that a backup is made of any existing archives using the LAS V3 2 Backup tool Do not delete archives before installing V3 3 as they can only be imported into V3 3 if they are left in an unchanged state LAS Release Notes Page 10 The use of archives is only possible when these are on the local drive of the PC or a mapped network drive to which the user has full access Sequence modules do not work with network drives Please note that if you are to install Leica AF6000 software on the same PC then it must be installed before LAS If you later reinstall Leica AF6000 it will be necessary to reinstall LAS afterwards Please note that if you are installing LAS V3 3 on a PC that already has Leica MM AF installed then the following message may appear during installati
28. ls in section 9 Can use a demo camera when no actual camera is available Vertical live scale bar Additional image formats and compression options Can specify number of decimal places in measurements Can apply different units e g change from um to mm Delete Rename archive Gallery image size can be adjusted by slider LAS Imaging Modules Enhancements to LAS Imaging Modules include e LAS Archive Basic o Search can be performed on text memo date numeric Boolean data fields e LAS Interactive Measurements o Measurement data file linked to record automatically o Circle displays diameter radius or area Further notes describing the new features are in Section 9 44 Version V3 0 1 release This release is a Steel Expert update only The reasons for this release are e To provide enhanced support for the EN 10247 standard of steel inspection e Improve the speed of data capture and processing e Enhance the usability of certain parts of Steel Expert in particular the manual rejection of inclusions e Improve the reporting of data by providing Excel templates for most of the standards supported e Improved the ability to analyse data in Excel by including positional information relative to the scan origin and optionally removing artefacts from the raw data e Miscellaneous other bug fixes and enhancements to Steel Expert 45 Version V3 0 0 release The LAS V3 0 0 release supported e The introduction of high performance
29. minimised As with any Internet related activity slow Internet connection or a high volume of traffic will affect the performance of the remote user s connection or live image refresh rate Web Sharing currently has no special security measures and the live or captured images can be viewed by anyone with access to the webpage address Too many remote user connections will slow performance of the LAS computer LAS Release Notes Page 30 Leica Microsystems Switzerland Ltd Telephone 44 1223 411411 Stereo and Macroscope Systems Fax 44 1223 412526 CH 9435 Heerbrugg Hotline 44 1223 401824 Switzerland di support leica microsystems com Www microscopy imaging com
30. n Please always draw the profile on one of the result images and not on the stack images to avoid this problem If using the LAS 3D Viewer please ensure that the manufacturer s latest graphics board drivers are installed particularly for ATI Radeon models LAS Release Notes Page 13 LAS MultiStep For Leica DFC500 only the 1 shot and 4 shot modes should be used LAS MultiTime Images with horizontal resolution greater than 2048 pixels cannot be saved in a movie file In practise movies are intended to be saved using a fast lower resolution mode in order to achieve a high frame rate If the cameras Leica DFC320 420 490 500 are used for movie recording please switch these to a binned or a progressive scan mode Please ensure that the manufacturer s latest graphics board drivers are installed particularly for ATI Radeon models LAS MultiTime Movie requires a Microsoft Windows XP patch file to be installed If your operating system language is one of the LAS supported languages the patch file appropriate to the language will be installed If you are using Windows with any other language you must download the patch file from Microsoft The effect of not installing this patch is that Movie files will not be compressed The following link works at the time of writing these release notes but you can otherwise search for the article KB904706 http www microsoft com downloads details aspx FamilyID 2636cfce 49ea 4d06 80ba 21a
31. nsity topography boundary position parameters Multiple Acceptance criteria Colour coding and labelling of feature results on screen Post measurement deletion of feature Freely defined Histogram Bar chart and Pie chart Export of results and images to customisable Excel reports Update to LAS Steel Expert This version introduces the following features e Significantly improved detection capabilities for coloured TiN inclusions e Separate results display for TiN inclusions e Use of 6 rather than 3 focus points for improved predictive focus LAS Release Notes Page 2 3 System Requirements 3 1 Supported Hardware The supported hardware is described in the document System Requirements that is in the root folder of the LAS Disc and is also installed under the Start gt Leica Application Suite menu 3 2 Computer System Requirements The recommended PC specification is described in the document System Requirements that is in the root folder of the LAS Disc and is also installed under the Start gt Leica Application Suite menu Please ensure that the PC specification corresponds to the recommendations made If using LAS with Windows Vista a suitable Windows Experience Index is 3 5 or higher Other factors that influence the performance of LAS are also noted in the same document 33 Computer Operation As well as the computer hardware and software components the performance will be influenced by the health of a PC If t
32. of mosaic imaging Use of LAS Power Mosaic with IsoPro stage must be the V2 stage requires that unidirectional scan is used for accurate stitching The recommended configuration is with Leica Z1GAPOA LAS Release Notes Page 14 63 Restrictions with LAS Applications LAS Image Organizer This module is replaced by LAS Folder Archive Basic For continued use of LAS Image Organizer please use LAS V2 8 Note that both LAS V3 and LAS V2 8 can be installed on the same PC You can start either V3 or V2 8 but you cannot start both at the same time 6 4 Computer Graphics Display When using Windows Vista the display mode will default to Aero We have experienced problems with some graphics boards drivers that stop LAS updating the display correctly For example the exposure slider does not update the accompanying numeric In another case the mouse cursor is erratic If this is noticed please try switching the Vista display to Basic You can see the difference in the opacity of the Windows dialogs With Aero they will be semi transparent and with Basic they will be solid The bit depth of the graphics display system of the PC must be set to 32 bits and support DirectX 9c DirectX 10 can be used with Windows Vista Hardware acceleration should be set to full or unpredictable artefacts may appear on the display when the mouse cursor or a window is moved Dual Monitors If you have not yet purchased your monitor and wish
33. on Please ignore this advice as LAS V3 3 is compatible with the newer version of the Sentinel Protection software that is detected Do NOT remove the newer version simply click OK and continue Sentinel Protection Installer 7 4 0 A A newer version was detected you must remove the newer version before you can continue Use of Mapped Network Drives Images can be stored directly to a mapped network drive provided you have appropriate permissions to use this Sequences cannot be stored to a network drive in order to preserve performance Note that speed of operation to a network will potentially be slow User Access to Folder Archives If a Folder Archive is in a location that is shared by a number of Windows users only one user at a time can access a Folder Archive This user must close LAS or select a different Archive to allow another user access Windows Limited User Restrictions If you are operating LAS as a Windows user not Administrator or Power User some folders are not available to you For example Shading Correction files are stored in the All User folder and cannot be stored by a limited user A limited user will need to be granted write access to this folder by an Administrator Similar issues can arise with annotation and measurement files and must be resolved in each case by a Windows administrator LAS Release Notes Page 11 Windows Font Selection The Fonts used by LAS require that the Cle
34. on the PC and for Office 2003 the Office Service Packs must be installed Office 2007 may also be used LAS MultiFocus LAS Montage and LAS MultiStep Memory Limits These modules use computer memory to store the sequence images For best performance they should be held in physical memory and not virtual paging or disc memory For example 50 images each of 5Mb would require 250Mb plus some additional working memory This is why the recommended minimum memory for use with these modules is 2Gb If your PC memory is 512Mb of which the operating software uses half it is easy to see that the physical memory can be exceeded If problems are encountered please increase the virtual memory by 1Gb and restart Windows However please be aware that the use of virtual memory severely impairs the speed of operation by a factor gt 10 and is not recommended If insufficient memory is available a warning will be given and the operation terminated The maximum practical file size for the Mosaic image created by LAS MultiStep is 500MB for which 2GB of PC memory is required LAS MultiFocus and LAS Montage When using the image alignment option with stereo images artefacts may be noticed at the edge of the MultiFocus image if the Z travel range is large Please note that the Profile line can be drawn on any image However if it is drawn on one of the stack images and the stack is from a non aligned source the profile may be measured from the wrong positio
35. onnect to as a web page You can copy this address right click and forward to a remote user via email Copy URL or test Web Sharing on your own system Open URL Web Sharing Image size Enable Copy URL Open URL Once enabled on the LAS system the remote user can now connect to the URL address The remote user cannot control the LAS live image or the camera but can see the LAS mouse pointer positions while it is within the live image viewer and also LAS Scale Bar Web Sharing will continue until it is stopped To stop or deactivate Web Sharing uncheck Enabled or close LAS 10 4 Remote User The remote user should open a Web Browser and type the URL address in the Web Page Address line as shown LAS Web Sharing Live Microsoft Internet Explorer File Edit View Favorites Tools Help E gt sl a A amp Search Jera Address 2 http 193 128 84 150 Las Get gt Microsoft yw Silverlight V If Microsoft Silverlight is not already installed on the remote system you will be automatically directed to the Microsoft website to download this component Follow the installation instructions and return the Browser to the Web Sharing address JavaScript must also be enabled in the Web Browser Once Silverlight has been installed the remote Web Sharing page should appear as LAS Release Notes Page 29 LAS Web Sharing Live Microsoft Internet Explo
36. per page dot And here are some examples from these The key point in creating these templates is to use Word tables and put a field into a table cell The image itself goes into an additional table within a table that has a single cell as this keeps the formatting correct LAS Release Notes Page 24 9 14 Licensing and Registration The registration information can be examined from LAS but cannot be changed while LAS is running About Leica Application Suite Camera Information LASHelp Registration Info For a PC that has been using a demo license for LAS V3 0 the modules Image Analysis Live Measurements Web Sharing and Reticule will not appear You must start LAS in license mode and install the demo license that ships with LAS V3 3 This will give a 60 day demo of these modules It will not give you any new demo period for the modules from LAS V3 0 9 15 License file name The license file name can now be changed provided it still ends in Iservrc 9 16 Reticule uses fit to window The Reticule module works within LAS and not as a separate application It appears whenever it is enabled by the licensing If you don t want to see it it can be turned off from the Registration screen The operation is described in the Help file Compared to LAS 2 8 1 Reticule no longer allows the overlay to be moved from Browse An improvement compare to LAS V2 8 1 is that fixed Reticule now scale to the live image resolution rath
37. professional image archiving e For Discovery II a new firmware update We recommend the update of the demo equipment Discovery II e g the new DM4 6000 stands for market introduction LAS Release Notes Page 6 e Update for camera software DFC Twain 6 10 0 support for Leica DFC360 FX e Minor fixes for the TL RCI and the IsoPro stage firmware LAS Archive Archives can be created to match the users needs in terms of image data and documentation The structure is versatile while the workflow orientation of LAS provides a deceptively simple user experience that makes it ideal for microscopy A virtually unlimited number of images and related data can be handled with high security The database consists of a hierarchy of levels that allows the record data to be conveniently organised into related categories All types of data may be linked to an image including text measurements dates microscope and camera settings All this data can be searched by simple and intuitive search methods for rapid retrieval of stored information The archiving capabilities of LAS are expressed by different optional Editions with increasing capability namely LAS Core LAS Basic and LAS Standard The features of these are LAS Core Features are similar to LAS V2 8 but use an SOL database and not folders for storing images other differences are e Database template is provided to store images and sequences with one sub level plus the image level Ex
38. puter access rights are synchronized between Windows and LAS The Windows user groups define users and these determine the access granted to an LAS user The correspondence is as follows LAS Release Notes Page 3 Windows User Level LAS access granted Windows Administrator Reauired for LAS installation Windows Administrator Complete access to all LAS features including or configuration Windows Power user or Windows user and member of Windows User Group LASAdministrator Windows user Access to LAS configuration features is not allowed The Windows user group LASAdministrator is established during the installation of LAS It is for use in organisations that restrict user access on computers Please ask your system administrator to add individual users to this group Accessing Hardware Setup The hardware setup application can be accessed either through LAS or by starting the executable directly a Starting from LAS In order to access the Hardware Setup from LAS then you need to either Become a member of the LASAdministrator group see above then start LAS as normal recommended Right click on the LAS desktop icon while holding down the Shift key and select Run as Admin if using Vista Run As for XP Enter the credentials of a user who belongs to the Admin group Continue to hold down the Shift key and when LAS starts select the Options menu item and then select Hardware S
39. rer BAX a Eile Edit View Favorites Tools Help Och amp x a Le E Search Sie Favorites 53 s kel Gi Si 33 Address http 193 128 84 150 Las sl cn un Leica Microsystems Web Sharing ee Acquire Image Restart Status Connected Seica MICROSYSTEMS Si Done Internet You can capture the image by pressing Acquire Image The captured image will be displayed in a new web page and can be saved by a right click over this image The image size will be the same as the size selected in the Captured Image Size in LAS If for some reason the remote live image fails or is slow to refresh you can press Restart or the browser refresh button This will temporarily stop and reload the page If there is a lot of internet traffic the live image may load intermittently When this happens the status will show as Buffering and the status dot in the top right corner will turn to yellow A red dot means the LAS web page is not available or has been stopped and a green dot means you are successfully connected Please note that the Web Sharing address is created by the network system of which the LAS computer is part and may change from time to time It is not necessarily always the same address from one day to the next To disconnect close the web browser or Web Sharing web page or move to another page 10 5 Limitations The remote user will not see the Web Sharing live image if LAS is
40. root folder of the LAS Disk You should read this document before installing your copy After installation the document can be accessed from the Start Menu gt Leica Application Suite Please read the document System Requirements pdf that is in the root folder of the LAS DVD for details of compatible hardware and software before continuing Installing hardware for use with LAS Power Mosaic is also described in the System Requirements pdf 72 Installing LAS Steel Expert This option is not installed by default In order to install LAS Steel Expert you need to select the Custom install if this is the initial installation of LAS or the Modify installation if you are adding to an existing installation Scroll down to find Steel Expert and change the symbol from a cross to a file system picture and OK to install Custom Setup Select the program features you want installed Click on an icon in the list below to change how a feature is installed Feature Description MultiStep a MultiTime Movie This feature requires OKB on your hard drive 73 Licensing Optional Modules Note if you are using LAS Core facilities only a license file is NOT required Do NOT start the evaluation period because this will give you a warning message for 60 days even if you do not evaluate any modules Optional modules may be purchased from your local Leica sales office The procedure in brief is as follows 1 Place the order
41. text fields to database User can rename fields in database Shows grid view and form view Allows selection of fields for display on data form Search for text in all fields of records and search for text in specified fields only Allows multiple files to be attached to record such as a word document Allows Sound file to be recorded attached to record and replayed Data limited by available PC memory no limit of images in database 12730207 LAS Folder Archive Standard Allows multi level database structure in a hierarchy of levels Allows new database structure to be created Text Memo Numeric Boolean Date Keyword fields Keyword list creation and use Any data from selected records can be output to a Word template Example Word templates are provided and can be customized by the user Output via Word template directly to pdf html and Word Example multi level databases provided 12730218 LAS Image Analysis Module Processing Sequence that can be rapidly adapted Run sequence automatically and Pause on any step in sequence Interactive adjustment of sequence step so the effects are immediately visible Split screen view for before and after image display Image smoothing and sharpening Image Delineation for accurate edge definition Thresholding colour and mono image Binary opening closing skeleton separation Binary editing for easy modification of the measurement mask Rejection and acceptance of measurement regions Size shape inte
42. to have a large screen area then we recommend a monitor with a resolution of 1920 x 1440 While a dual screen may be larger this single monitor option is very convenient because it avoids the centre gap between the monitors LAS is configured for display on a single monitor A dual monitor system should be configured as 2 separate monitors If the 2 monitors are configured as a single high resolution monitor the image will be divided by the gap between the monitors and hence is not convenient to use 6 5 Cameras Leica DFC500 Occasionally LAS does not shut down cleanly after using the DFC500 and the framework exe program has to be closed from the Task Manager or Windows restarted If this occurs please check if a later version of DFC Twain is available from DI Support Accessing camera features Some of the camera features are accessed from the Leica DFC Twain interface This displays the live image and allows camera settings to be adjusted and detailed adjustment to the camera operation Please refer to the Leica DFC Camera Acquisition Guide for further information Using Leica DFC280 Please do not use the combination of live image Full Frame and capture image Centre Scan as this causes as error message that effectively hangs LAS Leica DC Cameras Not supported LAS Release Notes Page 15 Dual Cameras with LAS LAS supports use of dual connected Leica DFC cameras but only allows one live image to be viewed at a time The Leica DF
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