Centrifuge Safety Manual
Contents
1. Lo In case of a power failure or if an emergency stop is required Turn power switch to OFF and disconnect the power source See manual for emergency door opening procedure 25 Care and Maintenance 1 Always maintain the rotor in the recommended manner The rotor and all accessories must be clean and inspected prior to each run do not use if rotor shows signs of corrosion or cracking See care and maintenance To avoid corrosion do not expose aluminum part of rotor to strong acids bases or alkaline laboratory detergents liquid chlorine bleach or salts of heavy metals When runs are not being made weekends and overnight allow the system to defrost by turning the power off or by leaving centrifuge door unlocked defrosting should take a few hours To prevent the samples from freezing operation should be limited to a setting of 4 or higher unless the system has been calibrated with the specific rotor and load Wash rotor components immediately if salts or other corrosive materials are used or spillage has occurred Do not allow corrosive materials to dry on the rotor Wipe instrument s vinyl finish with a damp cloth or wash with a mild detergent Rotors and sample buckets should be cleaned and disinfected after each use with a non corrosive cleaning solution mild detergent and distilled water are recommended Metal test tube brushes must not be used for cleaning the inside of the buckets All traces of detergent should be2. 13 Do not operate the centrifuge without installing the chamber bowl 14 Do not operate the rotor without the cover in position and locked in place and the rotor locked to the centrifuge drive 15 Select desired settings refrigeration temperature run time and brake rate 16 Press and release the START button 17 Do not lean or place items on the instrument while it is operating 18 Turn speed control to 10 and adjust as required to maintain desired speed 19 At end of run do not open chamber until the rotor comes to rest 20 When working with infectious materials wait 10 minutes after rotor has stopped before opening the centrifuge lid Be aware of decontamination and clean up procedures which apply to materials being centrifuged 21 1f a breakage has occurred wait at least 10 minutes after the rotor has stopped for aerosols and droplets to settle before opening lid 22 After the rotor comes to rest Open chamber door and remove the rotor Ensure rotor chamber is clean and dry Keep door closed between runs 23 Centrifugation causes a slight vacuum to build up in the tube cavity occasionally resulting in the suction effect when removing the tubes from the rotor Allow at least 5 minutes after the rotor comes to rest to remove the tubes from the rotor 24 Do not try to remove tubes by pulling the plugs 25 Do not hold onto or squeeze the tubes Tube contents will splash out when the plug is removed if pressure is applied to the tube
3. fill out and retain a record of all user names dates of training and instruments for which training was received ensure that the person who is responsible for each centrifuge carries out all the described safety tasks including regular maintenance servicing and record keeping ensure that any new centrifuge or any existing centrifuge that is moved to or from his her laboratory is properly disconnected moved and installed according to manufacturer specifications A service representative should be contacted to assist in moving and installing the instrument if required provide instructions involving the centrifugation of hazardous materials provide appropriate warning signs in rooms where potentially hazardous biological radioactive materials toxic or other hazardous chemicals are being centrifuged Lab Supervisor or Designated Employee It is the responsibility of the employee responsible for each centrifuge to User train new users in safe usage and maintenance of the centrifuge and supervise their use where necessary ensure that the safety procedures and centrifuge use log are kept close to the instrument provide copies of the user instructions including specifics on balancing loads in the rotor maximum G loads in RPM filling and sealing samples ensure all users fill out the centrifuge use log provide derating information on rotor based on centrifuge use log ensure that all the regular and periodic maintenance required is carri
4. Ensure rotor chamber is clean and dry Keep door closed between runs 11 When working with infectious materials wait 10 minutes preferably 30 minutes after rotor has stopped before opening the centrifuge lid Be aware of decontamination and clean up procedures that apply to materials being centrifuged 12 1f a breakage occurs wait at least 10 minutes after the rotor has stopped for aerosols and droplets to settle before opening the lid 13 Centrifugation causes a slight vacuum to build up in the tube cavity occasionally resulting in a suction effect when removing the tubes from the rotor Allow at least 5 minutes after the rotor comes to rest before removing the tubes from the rotor 14 Do not try to remove tubes by pulling the plugs 15 Do not hold onto or squeeze the tubes Tube contents will splash out when the plug is removed if pressure is applied to the tube Care and Maintenance 1 Always maintain the rotor in the recommended manner The rotor and all accessories must be clean and inspected prior to each run do not use if rotor shows signs of corrosion or cracking 2 To avoid corrosion do not expose aluminum part of rotor to strong acids bases or alkaline laboratory detergents liquid chlorine bleach or salts of heavy metals 3 Wash rotor components immediately if salts or other corrosive materials are used or spillage has occurred Do not allow corrosive materials to dry on the rotor 4 Immediate clean up of any blood splat
5. inform the person responsible for the centrifuge of the problem Inspect sample tubes and bottles for cracks or flaws prior to use Do not use if damaged Check the chemical compatibility of all materials used see table in manual See manual for specific instructions for filling sealing and capping tubes and bottles Ensure each tube is below maximum fill capacity to avoid leakage during centrifuging see manual for maximum tube capacity Excessively low liquid level may limit the rotor speed or crush the tube Do not interchange tube cap components They are designed as a unit for a particular tube being centrifuged in a particular rotor 10 To avoid corrosion do not expose aluminum part of rotor to strong acids bases or alkaline laboratory detergents liquid chlorine bleach or salts of heavy metals If exposure occurs clean the rotor immediately 11 Ensure samples are symmetrically placed in rotor and that loads are well balanced 12 Sample balancing can be done visually The difference between the meniscus level of the samples loaded in the same rotor must be within 5 mm 13 To prevent rotor failure the average density of liquids in each compartment should not exceed 1 7 g ml If it is exceeded the rotor speed should be reduced 14 To prevent rotor failure the rotor speed should be reduced whenever the rotor speed temperature combination exceeds the solubility of the gradient material and causes it to precipitate i e cesium ch
6. refer maintenance to qualified service personnel 13 Care and Maintenance 1 Check regularly that the motor and brake circuits are working properly 2 Always maintain the rotor in the recommended manner The rotor and all accessories must be clean and inspected prior to each run do not use if rotor shows signs of corrosion or cracking See care and maintenance 3 To avoid corrosion do not expose aluminum part of rotor to strong acids bases or alkaline laboratory detergents liquid chlorine bleach or salts of heavy metals 4 Wash rotor components immediately if salts or other corrosive materials are used or spillage has occurred Do not allow corrosive materials to dry on the rotor 5 Rotors and sample cups should be cleaned and disinfected after each use with a non corrosive cleaning solution mild detergent and distilled water are recommended Metal test tube brushes must not be used for cleaning inside of the cups All traces of detergent should be removed prior to air drying 6 The O ring should be cleaned with a tissue every 300 to 400 hours of use Replace the O ring if it becomes worn or damaged Lightly lubricate the O ring with silicone vacuum grease before reinstalling it to ensure an optimal vacuum seal 7 Keep chamber door closed between runs to prevent moisture and dirt from collecting inside Wipe off the chamber walls with a dry cloth before each run 8 Use the dry cycle to collect condensation after each cold run
7. refer to centrifuge use logs Microcentrifuges and other low speed centrifuges do not require use logs It is essential that a centrifuge use log be maintained for all ultracentrifuges high speed centrifuges and super speed centrifuges This is essential where manufacturers rotor derating systems apply reducing maximum speed to prevent rotor fatigue and other mechanical hazards Each user log entry must include the User s Name Date of Use Sample Description Number of Runs Run Time Run Speed Rotor Serial Number and Accumulated Revolutions for those instruments equipped with revolution counters A copy of the Centrifuge Safety Manual must be kept available near each centrifuge for easy access by users If a centrifuge is experiencing problems or maintenance is required the unit must be immediately taken out of service disconnected from the power source and a clearly marked DO NOT USE until serviced This notice will include the name of the person the date the reason and the signature of the lab supervisor or employee designate Maintenance 1 All centrifuge maintenance must be clearly documented in the log book provided in the lab for each unit This includes record of service history warranties and warranty expiration dates as well as details of maintenance carried out by laboratory users 2 If no reliable record of centrifuge history is found or records are inadequate or not up to date the unit concerned must be immediatel
8. rotor 21 Do not try to remove tubes by pulling the plugs 22 Do not hold onto or squeeze the tubes Tube contents will splash out when the plug is removed if pressure is applied to the tube In case of a power failure turn off the instrument and disconnect it from the main power source In case of a power failure lasting for several hours the samples must be retrieved from the rotor The control head cover and the front panel must be removed to disengage the door lock If possible refer maintenance to qualified service personnel Care and Maintenance 1 Always maintain the rotor in the recommended manner The rotor and all accessories must be clean and inspected prior to each run do not use if rotor shows signs of corrosion or cracking 2 To avoid corrosion do not expose aluminum part of rotor to strong acids bases or alkaline laboratory detergents liquid chlorine bleach or salts of heavy metals 3 Wash rotor components immediately if salts or other corrosive materials are used or spillage has occurred Do not allow corrosive materials to dry on the rotor 4 Wash only buckets of swinging rotors If necessary wipe off the rotor body with a damp cloth Do not pour water directly into rotor chamber 5 Rotors and sample buckets should be cleaned and disinfected after each use with a non corrosive cleaning solution mild detergent and distilled water are recommended Metal test tube brushes must not be used for cleaning the inside of
9. the buckets All traces of detergent should be removed prior to air drying 6 Keep chamber door closed between runs to prevent moisture and dirt from collecting inside Wipe off the chamber walls with a dry cloth before each run 7 A regular inspection of the unit should be performed before every use and a detailed inspection every 100 hours to inspect the rotor especially the inside cavities for rough spots cracks pitting white powder or deposits on aluminum rotors If any of these signs are evident do not run the rotor and call the manufacturer for servicing 8 Wipe clean O ring or gaskets regularly lubricate after cleaning Replace every 600 hours of use or as required 9 After 1000 runs or 2500 hours of centrifugation the maximum rated speed of all swinging bucket and aluminum fixed angle rotors should be permanently derated by 10 The overspeed device should also be replaced 10 Sample tubes bottles adapters and spacers should always be washed by hand using a mild detergent with brushes that will not scratch the tubes 11 Specific tube and bottle sterilization and disinfecting techniques based on material type can be found in the manual 12 Do not interchange tube cap components They are designed as a unit for a particular tube being centrifuged in a particular rotor 13 Centrifuges can suffer from rotor failure and other mechanical hazards as such they can be compromised by corrosion or fatigue Keep logs of maintenance an
10. tubes If breakage has occurred wait at least 10 minutes after rotor has stopped for aerosols and droplets to settle before opening the lid 19 If leakage has occurred follow all necessary precautions and use applicable decontamination procedures see lab safety procedures 20 Centrifugation causes a slight vacuum to build up in the tube cavity occasionally resulting in the suction effect when removing the tubes from the rotor Allow at least 5 minutes after the rotor comes to rest to remove the tubes from the rotor TIR ON LES OND In case of a power failure the power should always be turned off and the instrument disconnected from the main power source In case of a power failure lasting for several hours samples must be retrieved from the rotor Be sure the rotor has stopped rotating before attempting to open it Use the cover interlock bypass see manual Care and Maintenance 1 Always maintain the rotor in the recommended manner The rotor and all accessories must be clean and inspected prior to each run do not use if rotor shows signs of corrosion or cracking Replace the affected parts immediately 2 To avoid corrosion do not expose aluminum part of rotor to strong acids bases or alkaline laboratory detergents liquid chlorine bleach or salts of heavy metals Oot D 21 Always unplug unit before cleaning Release latch open cover check chamber for spillage or broken glass prior to use If present and materials a
11. 9 The fins on the refrigeration condenser must be clean for efficient heat transfer Use a vacuum cleaner or damp cloth to clean the fins Be careful the fins are sharp 10 The vacuum pump oil should be changed every 400 600 hours of use or whenever oil is cloudy 11 A regular inspection of the unit should be performed before every use and a detailed inspection every 100 hours of use to inspect the rotor especially the inside cavities for rough spots cracks pitting white powder or deposits on aluminum rotors If any of these signs are evident do not run the rotor and call the manufacturer for servicing 12 Sample tubes bottles adapters and spacers should always be washed by hand using a mild detergent with brushes that will not scratch the tubes 13 Do not interchange tube cap components They are designed as a unit for a particular tube being centrifuged in a particular rotor 14 Centrifuges can suffer from rotor failure and other mechanical hazards as such they can be compromised by corrosion or fatigue Keep logs of maintenance and use for centrifuges Contact the centrifuge manufacturer and use the centrifuge log for preparing the derating schedule of the rotor 14 Sorvall RC M150GX Micro Ultracentrifuge Standard Operation 1 Turn power switch on Use only Sorvall rotors and accessories designed for use in this instrument Inspect the rotor for signs of corrosion or cracking before using If found do not use the rotor and
12. Centrifuge Safety Manual General Information The following program must be followed when using centrifuges to ensure that all centrifuges in campus laboratories are used cared for and maintained in a safe manner Training Ay It is the responsibility of the lab supervisor to ensure that all centrifuge users are properly trained in the selection care and use of centrifuge accessories and rotors The lab supervisor may delegate training to the employee responsible for the centrifuge involved Use of centrifuges by new users must be closely supervised by the employee responsible for each centrifuge until he she is satisfied that unsupervised usage of the instrument is safe All users must be familiar with the safety procedures for all of the centrifuges that they use including proper load selection balancing and maintenance of rotor where necessary It is the responsibility of the laboratory supervisor to ensure that each user has carefully read and understands the Centrifuge Safety Instructions specific for that centrifuge prior to instrument use Users must sign a copy of these instructions to ensure that they have read and understand them Use 1 All centrifuges that have manufacturers rotor derating systems including ultracentrifuges high speed centrifuges Sorval and equivalent instruments and high speed benchtop Eppendorf IEC Jouan and equivalent instruments must have an up to date record of the total hours of usage
13. an lead to severe personal injury e Use only rotors designed for use in this instrument e Inspect the rotor for signs of corrosion or cracking before using If found do not use the rotor and inform the employee responsible for the centrifuge of the problem e Inspect the inter lock system to ensure the cover cannot be opened while the rotor is spinning e Never operate the rotor unless it is symmetrically loaded and balanced Care is required to achieve this e Never operate the rotor without the lid or cover closed and locked in place if the lid cannot be locked the machine must be removed from service e Never exceed the maximum recommended speed of the rotor e Clean and disinfect rotors and sample cavities or cups after each use with non corrosive solutions 3 Sample management is also very important to safety Lack of care can result in exposure of the user to harmful materials e Always use sample tubes or bottles designed for the particular rotor being used e In general samples should be capped to avoid generation of aerosols e Nitrocellulose tubes should only be used when transparent and flexible They must never be heated because of explosion possibility 5 Plastic centrifuge tubes should be discarded after one cycle of ultracentrifugation The failure rate for used tubes is a hazard that justifies using new tubes for each high G run When using radioactive toxic or pathogenic materials be aware of potential hazards
14. and accessories designed for use in this instrument 2 Inspect the rotor for signs of corrosion or cracking before using If found do not use the rotor and inform the person responsible for the centrifuge of the problem 3 Inspect sample tubes for cracks or flaws prior to use Do not use if damaged 4 Do not interchange tube cap components as they are designed as a unit for a particular tube being centrifuged in a particular rotor 5 Do not overfill tubes that require plugs or caps overfilling can cause overflow when plug or cap is inserted However too much air can cause the tube to deform and disrupt gradients and sample bands as well as increasing the force required to remove the tube from the cavity after centrifugation 6 To avoid corrosion do not expose aluminum part of rotor to strong acids bases or alkaline laboratory detergents liquid chlorine bleach or salts of heavy metals If exposure does occur clean the rotor immediately 7 Ensure samples are symmetrically placed in rotor and that bucket loads are well balanced The instrument will automatically shut off if the unbalance is excessive 8 Rotor must not be run with buckets missing in case of rotors with free inserts adapters or swing buckets 9 Never mix buckets from different sets on the same rotor in case of rotors with free inserts adapters or swing buckets 10 Do not pour samples directly into buckets or tube holders 11 Ensure the rubber pads or adapters suppli
15. associated with them in case of leakage during centrifugation If leakage does occur you may be exposed to particles dispersed in the air aerosol It is recommended that additional precautions be taken to prevent exposure to these materials such as the use of controlled ventilation or isolation areas If exposure occurs to radioactive toxic or pathogenic materials all necessary precautions and appropriate decontamination procedures should be used see lab safety procedures for toxic pathogenic and Radiation Safety Guide for radioactive Dispose of all waste solutions according to appropriate health and safety guidelines 4 For safe use of the centrifuge Do not circumvent any of the safety features such as lid closure override switches They are there to protect you Do not lean or place items on the instrument while it is operating Do not leave the centrifuge until full operating speed is attained and the instrument appears to be running normally without vibration If vibration occurs stop the run immediately wait until the rotor stops and check the load balances In event of a power failure do not try to open the lid to retrieve samples for at least one hour After the rotor has stopped follow the instructions in the manual for recovery of the samples Instrument User Log Rotor Use Log Instrument Maintenance Log Specific Safety Instructions Beckman Optima Le 80K Preparative Ultracentrifuge Power If the power to the in
16. ckets swing freely 9 Never leave a damp rotor on a metal surface particularly stainless steel because an electrochemical reaction could take place with the aluminum or magnesium in the rotor Causing corrosion 10 Clean the rotor chamber and the accessories with a cotton wool pad dipped in 70 alcohol after 30 hours of use After cleaning rinse with clean water and dry 11 A regular inspection of the unit should be performed before every use and a detailed inspection after 100 hours of use to inspect the rotor especially the inside cavities for rough spots cracks pitting white powder or deposits on aluminum rotors If any of these signs are evident do not run the rotor and call the manufacturer for servicing 12 After every 200 hours of use check that the centrifuge can attain the maximum r p m possible for your rotor see table 3 3 in manual 13 After every 200 hours of use check that rotor chamber air temperature is within 1 C of temperature readout 14 Sample tubes bottles adapters and spacers should be washed by hand if possible using a mild detergent with brushes that will not scratch the tubes Do not use a metal brush 15 Centrifuges can suffer from rotor failure and other mechanical hazards as such they can be compromised by corrosion or fatigue Keep logs of maintenance and use for centrifuges Contact the centrifuge manufacturer and use the centrifuge log for preparing the derating schedule of the rotor 20 IEC Micromax Ce
17. components immediately if salts or other corrosive materials are used or spillage has occurred Do not allow corrosive materials to dry on the rotor 3 Before opening cleaning the rotor chamber open the lid of the centrifuge and disconnect the main power plug Unscrew the rotor with the hexagon key and clean separately The rotor chamber should only be cleaned with a moist cloth and a neutral detergent after every 100 hours of use The rotor retainer should be lubricated using Vaseline spray inside and outside of cone 4 The rubber seals in the rotor chamber should be rinsed well with water and lubricated with glycerin after every 100 hours of use 5 Motor shaft should be lubricated after every 100 hours of use using pivot grease Need to take rotor out first 6 Check rotor and housing for residue and corrosion after every 100 hours of use 7 Check magnetic ring on the base of the rotor regularly for damage and have it replaced if necessary 8 When using swing buckets ensure the grooves in which the buckets are fitted and are free of contamination The buckets can be lubricated with the grease supplied if necessary 9 The sealing rings of the aerosol tight rotor FA 45 30 11 should be checked and replaced regularly 10 All rotors are autoclavable temperature up to 121 C After the rotor has been autoclaved 20 times the seals of the aerosol buckets should be replaced 11 A regular inspection should be performed before every use and at a
18. ct the rotor for signs of corrosion or cracking before using If found do not use the rotor and inform the person responsible for the centrifuge of the problem 4 Inspect tubes and bottles for cracks or flaws prior to use Do not use if damaged 5 Do not interchange tube cap components They are designed as a unit for a particular tube being centrifuged in a particular rotor 6 Minimize the differences in weight of the filled sample tubes Refer to instruction manual for details 7 The maximum recommended weight of a filled bucket is stated on each rotor 8 Excessively low liquid level may limit the rotor speed or crush the tube Refer to instruction manual for details 9 To avoid corrosion do not expose aluminum part of rotor to strong acids bases or alkaline laboratory detergents liquid chlorine bleach or salts of heavy metals If exposure does occur clean the rotor immediately 10 Rotation speed must be reduced below the maximum for liquids with a density of 1 2 g ccm or higher see manual 11 Before running a swinging bucket rotor make sure that each is mounted correctly and that they can swing freely Ensure grooves are clean before inserting buckets Unclean grooves and pivots prevent buckets from swinging freely 12 Ensure samples in rotor are symmetrically placed in rotor and that the load is balanced Refer to the instruction manual for details of how to achieve 13 Install rotor Close and lock lid 14 Enter the required
19. d use for 11 centrifuges Contact the centrifuge manufacturer and use the centrifuge log for preparing the derating schedule of the rotor Beckman L8 70M Ultracentrifuge Standard Operation ONS 8 9 10 11 12 13 14 15 16 17 18 19 20 21 Use only the Beckman rotors and accessories designed for use in this instrument Inspect bottles and tubes for cracks or flaws prior to use Do not use if damaged See manual for specific instructions for filling sealing and capping tubes and bottles Do not overfill tubes that require plugs overfilling can cause overflow when plug is inserted however too much air can cause the tube to deform and disrupt gradients and sample bands as well as increasing the force required to remove the tube from the cavity after centrifugation Unless otherwise specified the minimum recommended volume for bottles is half full this will reduce rotor speed for optimum performance Maximum and minimum fill levels and cap requirements should be referred to in table 3 6 of the manual Ensure samples are symmetrically placed in rotor With power on open the chamber door install the rotor and close and lock the chamber door Enter the required run conditions Vacuum Speed Temp Max Temp and Time Hold or o t as outlined in the manual Select slow ACCEL and or DECEL profiles if desired Do not exceed rotors recommended maximum run speed Do not lean or place items on the ins
20. e rotor Secure the rotor top if this is supplied with the rotor Close the lid Enter the required run conditions by setting the timer and the rpm indicators Press the start button Run continues for the specified time or can be terminated by setting the timer to 0 or by pressing the stop button Be aware of decontamination and cleanup procedures that apply to the materials being centrifuged Care and Maintenance als Keep the interior of the rotor chamber and the rotor clean and dry Remove the rotor and use water and a mild detergent to clean the interior surfaces and the rotor Dry all surfaces then replace the rotor Follow the instrument manufacturer s instructions in removing and replacing the rotor Microcentrifuge failure normally occurs as a result of electrical circuit board failures or motor wear If you detect a failure to run failure to attain normal operating speed failure to engage the self locking device or to disengage the device at the end of the run other electrical faults unusual motor operating noise or any other sign of failure report the problem to the laboratory supervisor or employee designate Laboratory supervisors must maintain microcentrifuges in good operating condition by ensuring that units are serviced when operating faults are detected
21. ed out and recorded in the maintenance log contact a service representative when repair is necessary and record repair details in centrifuge maintenance log It is the responsibility of the user to attend a mandatory training session on centrifuge lab safety use care and maintenance before being allowed to operate a centrifuge operate and maintain all centrifuges in accordance with NIEHS policy and good safe laboratory procedures safety procedures as required Read and follow all instructions for safe usage and maintenance of the centrifuge Should users at any time be unsure of the safe operation of the instrument they must refer to the instruction manual for details and or ask the employee responsible for the centrifuge or the laboratory supervisor for assistance fill out the log each time the instrument is used Report damage to centrifuge or rotor to the laboratory supervisor so action in terms of repair or derating may be performed Mechanical Breakdown In the event of a mechanical breakdown such as rough running a broken or chipped rotor the rotor is off the spindle during use a dropped rotor or other types of mechanical problems the user must notify the lab supervisor or employee designate immediately Emergency Procedure In the event that an incident or accident related to centrifugation occurs turn off centrifuge and disconnect it from the power source notify others in laboratory and evacuate notify
22. ed with specific tube holders are installed into the cavity in order to cushion the tube 12 Ensure all buckets on swing bucket rotors are allowed to move freely and are properly seated in the hinge pins 13 With power on open the chamber door install the rotor and lock rotor to shaft and close the chamber door 14 The centrifuge will not operate until the lid is closed and latched in place The lid remains latched until the rotor stops spinning 15 Precool bowl and rotor if desired 16 Select desired program with speed temperature and time 17 Press the START button 18 Press STOP to terminate a run at any time 19 Make sure run is proceeding normally before leaving the area 20 At end of run do not open chamber until the rotor comes to rest 21 When working with infectious materials wait 10 minutes after rotor has stopped before opening the centrifuge lid Be aware of decontamination and clean up procedures which apply to materials being centrifuged 22 1f a breakage has occurred wait at least 10 minutes after the rotor has stopped for aerosols and droplets to settle before opening lid 23 Centrifugation causes a slight vacuum to build up in the tube cavity occasionally resulting in the suction effect when removing the tubes from the rotor Allow at least 5 minutes after the rotor comes to rest to remove the tubes from the rotor 24 Do not try to remove tubes by pulling the plugs or unscrewing the caps 19 25 Do not hold on
23. es from the rotor When this is happening allow at least 5 minutes after the rotor comes to rest before removing the tubes from the rotor 30 Do not try to remove tubes by pulling the plugs or unscrewing caps 31 Do not hold onto or squeeze the tubes Tube contents will splash out when the plug or screw cap is removed if pressure is applied to the tube In case of a power failure the power should always be turned off and the unit disconnected from the main power source In case of a power failure lasting for several hours samples must be retrieved from the rotor Follow instructions in the manual for emergency recovery from power outage Ensure that the rotor is stopped before removing the cover If possible refer maintenance to qualified service personnel Care and Maintenance 1 Check regularly that the motor and brake circuits are working properly 2 Wash rotor components immediately if salts or other corrosive materials are used or spillage has occurred Do not allow corrosive materials to dry on the rotor 3 Do not autoclave aluminum covers or rotor body Aluminum must not be exposed to temperatures greater than 121 C 4 Wipe the magnet surface on the bottom of the rotor to remove any iron powder accumulation 5 A regular inspection of the unit should be performed before every use and a detailed inspection after every 100 hours of use to inspect the rotor especially the inside cavities for rough spots cracks pitting white
24. ged 6 Do not spin fluids having a specific gravity greater than 1 5 7 Do not use instrument with concentrated mineral acids such as hydrochloric sulfuric or nitric acid 8 Avoid overfilling tubes 9 Ensure samples are symmetrically placed in rotor 10 Close and latch cover Do not operate the rotor without the cover in position and latched in place 11 Set POWER to ON Slight vibration and noise are normal 12 Do not lean or place items on the instrument while it is operating 13 If vibration is excessive set POWER to OFF position wait until rotor completely stops and then check that the load is correctly balanced 14 To stop run set POWER to OFF position Do not use the cover latch as a start stop switch 15 Wait until rotor comes to a full stop and then unlatch and open the cover 16 When working with infectious materials wait 10 minutes after the rotor has stopped before opening the centrifuge lid Be aware of decontamination and clean up procedures which apply to materials being centrifuged 17 Ensure no leakage or glass breakage has occurred before removing tubes If breakage has occurred wait at least 10 minutes after the rotor has stopped for aerosols and droplets to settle before opening the lid 18 1f leakage has occurred follow all necessary precautions and use of appropriate decontamination procedures if applicable should be taken see lab safety procedures 19 Centrifugation causes a slight vacuum to build up
25. igue Keep logs of maintenance and use for centrifuges Contact the centrifuge manufacturer and use the centrifuge log for preparing the derating schedule of the rotor 16 Sorvall Superspeed Fixed Angle Centrifuge RC 5C PLUS RC 5B RC 2B Standard Operation 1 Turn power switch on Use only Sorvall rotors and accessories designed for use in this instrument Inspect the rotor for signs of corrosion or cracking before using If found do not use the rotor and inform the person responsible for the centrifuge of the problem Inspect sample tubes and bottles for cracks or flaws prior to use Discard if damaged Check the chemical compatibility of all materials used see table in Appendix Ensure each tube is below maximum fill capacity to avoid leakage during centrifuging Do not interchange tube cap components They are designed as a unit for a particular tube being centrifuged in a particular rotor 8 To avoid corrosion do not expose aluminum part of rotor to strong acids bases or alkaline laboratory detergents liquid chlorine bleach or salts of heavy metals 9 Ensure maximum compartment mass of rotor is not exceeded includes specimen tubes cover and adapter to prevent rotor failure if exceeded rotor speed must be reduced 10 Open chamber door load rotor 11 1f temperature is important for samples precool the chamber first 12 Ensure samples in rotor are symmetrically placed in rotor and loads are balanced 13 Ensure roto
26. in the tube cavity occasionally resulting in the suction effect when removing the tubes from the rotor Allow at least 5 minutes after the rotor comes to rest before removing the tubes from the rotor Care and Maintenance 1 Always maintain the rotor in the recommended manner The rotor and all accessories must be clean and inspected prior to each run do not use if rotor shows signs of corrosion or cracking Replace the affected parts immediately 2 To avoid corrosion do not expose aluminum part of rotor to strong acids bases or alkaline laboratory detergents liquid chlorine bleach or salts of heavy metals 3 Always unplug unit before cleaning 4 Release latch open cover check chamber for spillage or broken glass If present and materials are non toxic non radioactive and non pathogenic mop up with a sponge dampened with water OS 23 Rotors and sample cups or cavities should be cleaned and disinfected after each use with a non corrosive cleaning solution mild detergent and distilled water are recommended Metal test tube brushes must not be used for cleaning the cup cavities All traces of detergent should be removed prior to air drying Never pour water directly into chamber Check that vent holes in the cover and in the chamber bottom are not blocked Should parts need replacement follow detailed instructions provided in manual or contact a repair technician Keep logs of maintenance and use of centrifuge 24 Bec
27. inet panels after every 100 hours of use 7 Clean condenser fins once every 100 to 300 hours of use or whenever dust accumulates visibly 8 Replace the motor brushes whenever the BRUSHES advisory message lights up 9 Clean the motor exhaust filter each time the brushes are replaced 10 Rotor should be stored clean dry upside down with cover and tubes removed this will allow air to circulate 11 Do not pour water directly into rotor chamber 12 A regular inspection of the unit should be performed before every use and a detailed inspection every 100 hours to inspect the rotor especially the inside cavities for rough spots cracks pitting white powder or deposits on aluminum rotors If any of these signs are evident do not run the rotor and call the manufacturer for servicing 13 Sample tubes bottles adapters and spacers should be washed using warm water a mild detergent and brushes that will not scratch the tubes Do not use metal brushes 14 Specific tube and bottle sterilization and disinfecting techniques based on material type can be found in the manual 15 Centrifuges can suffer from rotor failure and other mechanical hazards as such they can be compromised by corrosion or fatigue Keep logs of maintenance and use for centrifuges Contact the centrifuge manufacturer and the use centrifuge log for preparing the derating schedule of the rotor 18 Jouan MR 22l Centrifuge Standard Operation 1 Use only recommended rotors
28. kman TJ 6R Tabletop Centrifuge Standard Operation 1 Use only the Beckman rotors and accessories designed for use in this instrument 2 Inspect tubes for cracks or flaws prior to use Do not use if damaged 3 Do not overfill tubes that require plugs overfilling can cause overflow when plug is inserted however too much air can cause the tube to deform and disrupt gradients and sample bands as well as increasing the force required to remove the tube from the cavity after centrifugation 4 Ensure samples are symmetrically placed in rotor and that bucket loads are well balanced The instrument will automatically shut off if the unbalance is excessive Rotor must not be run with buckets missing Do not pour samples directly into buckets or tube holders Ensure the rubber pads supplied with each tube holder are installed into the cavity in order to cushion the tube 8 Ensure all buckets are allowed to move freely and are properly seated in the hinge pins 9 For fixed angle rotors load the rotor symmetrically so that the opposing weights are within 10 grams of one another When the rotor spins the air pressure differential will hold the lid firmly in place 10 Heavier loads may be used provided the rotor is run at a lower speed See formula in manual for speed derating 11 With power on open the chamber door install the rotor and close and lock the chamber door 12 Do not bend the drive shaft when installing or removing the rotor
29. loride 15 Before running a swinging rotor make sure that each bucket is properly seated on its support pins 16 Some tubes and adapters will not operate at the maximum speed of the rotor Refer to manual 17 Ensure samples in rotor are symmetrically placed in rotor 18 Do not operate keyboard of unit with ballpoint pen or any other sharp object 19 If sample temperature control is important you should equilibrate the sample temperature before the run 20 Install rotor Close and lock chamber door 21 Ensure rotor cover is properly locked in place before operating rotor rotors are equipped with double locking knobs on cover 22 Enter the required run conditions 23 Do not exceed the maximum recommended speed for the rotor 24 Press vacuum key 25 Press START key Run operates for specified operation time or press STOP key 26 Do not open the cover until the rotor has stopped Once rotor has stopped press VACUUM key take rotor out 27 When working with infectious materials wait 10 minutes after rotor has stopped before opening the centrifuge lid Be aware of decontamination and clean up procedures that apply to materials being centrifuged 28 If a breakage has occurred wait at least 10 minutes for aerosols and droplets to settle before opening lid ON ores 00 15 29 Centrifugation causes a slight vacuum to build up in the tube cavity occasionally resulting in the suction effect when removing the tub
30. minimum of 100 hours of use to inspect the rotor especially the inside cavities for rough spots cracks pitting on rotors If any of these signs are evident do not run the rotor and call the manufacturer for servicing 12 Sample tubes bottles adapters and spacers should always be washed by hand if possible using a mild detergent with brushes that will not scratch the tubes Do not use metal brushes 13 Centrifuges can suffer from rotor failure and other mechanical hazards as such they can be compromised by corrosion or fatigue Keep logs of maintenance and use for centrifuges Contact the centrifuge manufacturer and the use centrifuge log for preparing the derating schedule of the rotor 28 Clinaspin Model 8C Standard Operation Use only recommended rotors and accessories designed for use in this instrument Inspect tubes and bottles for cracks or flaws prior to use Do not use if damaged Open chamber door load rotor Ensure samples in rotor are symmetrically placed Ensure buckets are properly seated in the hinge pins and allowed to move freely Ensure rubber pads are in place in the buckets 6 Ensure rotor cover is on Close and lock chamber door 7 Specimens should be run at 3500 RPMs for 15 20 minutes 8 Do not lean or place items on the instrument while it is operating 9 1 IR OO ES To stop run turn speed control knob to zero 0 Wait for rotor to come completely to rest Open chamber door and remove the rotor
31. ntrifuge Standard Operation Switch ON OFF button to turn unit on Press the STOP OPEN cover button to release the cover interlock Ensure that rotor turns freely and that there are no loose objects in the chamber Ensure the centrifuge chamber is clean and dry Ensure the appropriate tube size is employed Use only IEC accessories designed for use in this instrument Inspect tubes for possible cracks or flaws Do not use if damaged If possible use capped tubes to contain samples and prevent aerosols Avoid overfilling tubes Ensure samples are symmetrically placed in rotor 10 Close cover and press down until it latches 11 Set desired time speed and temperature if unit is RF model and then press the start button 12 Never spin tubes faster than their recommended G force and never centrifuge disposable tubes more than once 13 Do not use instrument with concentrated mineral acids such as hydrochloric sulfuric or nitric acid 14 Rotor will accelerate to set speed spin for set time and brake to a stop 15 Wait until rotor comes to a full stop and then unlatch and open the cover 16 Press the STOP OPEN cover button to release the safety interlock 17 When working with infectious materials wait 10 minutes after rotor has stopped before opening the centrifuge lid Be aware of decontamination and clean up procedures which apply to materials being centrifuged 18 Ensure no leakage or glass breakage has occurred before removing
32. powder or deposits on aluminum rotors If any of these signs are evident do not run the rotor and call the manufacturer for servicing 6 Rotor should be stored clean dry upside down with cover and tubes removed this will allow air to circulate 7 Rotor chamber should be kept clean and wiped dry routinely to maintain efficient vacuum and cooling Wash chamber with warm water and mild detergent at least once for every 30 hours of use rinse the chamber and dry it with soft absorbent cloth Ideally after each use 8 Wipe the drive spindle with a dry soft cloth before every run to reduce the chance of the rotor sticking to the spindle 9 Wipe door O ring clean regularly lubricate after cleaning to ensure high vacuum If door is frequently used take out O ring after every 300 to 400 hours of use wipe it with a soft cloth and apply a thin coat of vacuum grease Clean the door seal O ring with a clean soft cloth dampened with alcohol 10 Use a mild non alkaline detergent and water or a household wax cleaner to clean cabinet parts 11 Sample tubes bottles adapters and spacers should be washed using warm water a mild detergent and brushes that will not scratch the tubes Do not use metal brushes 12 Specific tube and bottle sterilization and disinfecting techniques based on materials can be found in the manual 13 Centrifuges can suffer from rotor failure and other mechanical hazards as such they can be compromised by corrosion or fat
33. r cover is on Close and lock chamber door rotors are equipped with double locking knobs on cover 14 Do not exceed the maximum recommended speed of the rotor The rotor speed must be reduced if the rotor speed temperature combination exceeds the solubility of the gradient material and causes it to precipitate 15 Enter the required run conditions speed time brake For the RC5C Plus the rotor code must match the rotor 16 Press START 17 After run has stopped or after pressing stop to end run wait for rotor to come completely to rest Open chamber door remove rotor cover then remove sample tubes or bottles from rotor If rotor is not being used again remove rotor from chamber Ensure rotor chamber is clean and dry keep door closed between runs 18 When working with infectious materials wait 10 minutes after rotor has stopped before opening the centrifuge lid Be aware of decontamination and clean up procedures that apply to materials being centrifuged 19 1f a breakage occurs wait at least 10 minutes after the rotor has stopped for aerosols and droplets to settle before opening the lid 20 Keep the chamber door closed after the rotor is removed to inhibit formation of condensation on walls Remove rotor as soon as possible to avoid samples from freezing 21 Centrifugation causes a slight vacuum to build up in the tube cavity occasionally resulting in the suction effect when removing the tubes from the rotor Allow at least 5 minute
34. r o t as outlined in the manual 9 Instrument should be set for standard or programmed operation see manual 10 Do not exceed rotors maximum run speed 11 Press ENTER RECALL and START the vacuum system is automatically activated unless you pressed VACUUM earlier 12 Do not lean or place items on the instrument while it is operating 13 Make sure run is proceeding normally before leaving the area 14 Press stop to terminate a run in the HOLD mode or to stop for any reason Runs in timed and w t modes will terminate automatically and the instrument will sound a tone to indicate end of run 15 At end of run do not open chamber until the rotor comes to rest 16 After rotor has stopped press VACUUM to vent chamber 17 When working with infectious materials wait 10 minutes after the rotor has stopped before opening the centrifuge lid Be aware of decontamination and clean up procedures that apply to materials being centrifuged 18 If a breakage has occurred wait at least 10 minutes after the rotor has stopped for aerosols and droplets to settle before opening lid 10 19 Open chamber door and remove the rotor Ensure rotor chamber is clean and dry Keep door closed between runs 20 Centrifugation causes a slight vacuum to build up in the tube cavity occasionally resulting in the suction effect when removing the tubes from the rotor Allow at least 5 minutes after the rotor comes to rest before removing the tubes from the
35. re non toxic non radioactive and non pathogenic mop up with a sponge dampened with water Rotors and sample cups or cavities should be cleaned and disinfected after each use with a non corrosive cleaning solution mild detergent and distilled water are recommended Do not use acetone Metal test tube brushes must not be used for cleaning the cup cavities All traces of detergent should be removed prior to air drying Never pour water directly into chamber After cleaning any part dry it properly preferably using a clean absorbent towel Should parts need replacement contact a repair technician Specific tube and bottle sterilization can be done by autoclaving Remove caps stoppers and other tube closures before autoclaving to keep tubes from collapsing under pressure 10 Keep logs of maintenance and use of centrifuge 22 IEC Spinette Model 4 6 or 12 Centrifuge Standard Operation 1 Set POWER switch to OFF position Unlatch and open cover Ensure that rotor turns freely and that there are no loose objects in the chamber and that the rubber suction cup mounting feet on the bottom of the unit are not worn or missing 2 Ensure the centrifuge bowl and tubes are dry 3 Ensure the appropriate tube size is employed Use only Spinette accessories designed for use in this instrument 4 If possible use capped tubes to contain samples and prevent aerosols 5 Inspect tubes and bottles for possible cracks or flaws Do not use if dama
36. removed prior to air drying Keep chamber door closed between runs to prevent moisture and dirt from collecting inside Wipe off the chamber walls with a dry cloth before each run A regular inspection of the unit should be performed before every use and after 100 hours of use to inspect the rotor especially the inside cavities for rough spots cracks pitting white powder or deposits on aluminum rotors If any of these signs are evident do not run the rotor and call the manufacturer for servicing 10 Drive belt and motor brush replacement when required should be performed by trained personnel 11 Sample tubes bottles adapters and spacers should always be washed by hand using a mild detergent with brushes that will not scratch the tubes 12 Inspect tubes and bottles for cracks and any major deformities before use 13 Do not interchange tube cap components they are designed as a unit for a particular tube being centrifuged in a particular rotor 14 Centrifuges can suffer from rotor failure and other mechanical hazards as such they can be compromised by corrosion or fatigue Keep logs of maintenance and use for centrifuges Contact the centrifuge manufacturer and the use centrifuge log for preparing the derating schedule of the rotor 26 Eppendorf Centrifuge 5804 5804 R and 5810 5810 R Standard Operation 1 Turn power switch on 2 Use only Eppendorf rotors and accessories designed for use in this instrument 3 Inspe
37. run conditions 15 Press START key Run operates for specified operation time or press STOP key to stop 16 Do not use the lid closure override to open the instrument before the rotor has stopped Wait until the rotor has stopped before opening the lid to remove samples 17 When working with infectious materials wait 10 minutes after the rotor has stopped before opening the centrifuge lid Be aware of decontamination and clean up procedures which apply to materials being centrifuged 18 1f a breakage has occurred wait at least 10 minutes after the rotor has stopped for aerosols and droplets to settle before opening lid 19 Centrifugation may cause a slight vacuum to build up in the tube cavity occasionally resulting in the suction effect when removing the tubes from the rotor When this occurs allow at least 5 minutes after the rotor comes to rest before removing the tubes from the rotor 20 Do not try to remove tubes by pulling the plugs or unscrewing caps 21 Do not hold onto or squeeze the tubes Tube contents will splash out when the plug or cap is removed if pressure is applied to the tube In case of a power failure disconnect machine from the power source In case of a power failure lasting for several hours the samples must be retrieved from the rotor Ensure rotor has stopped and activate emergency lid release 27 Care and Maintenance _ Check regularly that the motor and brake circuits are working properly 2 Wash rotor
38. s after the rotor comes to rest before removing the tubes from the rotor 22 Do not try to remove tubes by pulling the plugs or unscrewing caps 23 Do not hold onto or squeeze the tubes Tube contents will splash out when the plug or screw cap is removed if pressure is applied to the tube wh PE OPEN ES 17 24 The last user of the day should leave the lid open to allow condensation to evaporate In case of a power failure turn off the instrument and disconnect it from the main power source In case of a power failure lasting for several hours the samples must be retrieved from the rotor The control head cover and the front panel must be removed to disengage the door lock Always wait until the rotor has stopped before opening the door If possible refer maintenance to qualified service personnel Care and Maintenance 1 Check regularly that the motor and brake circuits are working properly 2 Wash rotor components immediately if salts or other corrosive materials are used or spillage has occurred Do not allow corrosive materials to dry on the rotor 3 Wash rotor and cups or cavities with warm water and mild detergent after each use Do not use metal test tube brushes for cleaning cups or cavities All traces of detergent should be removed prior to air drying 4 The rotor chamber should be defrosted and cleaned periodically to maintain efficient cooling 5 Wash the tapered spindle after every 30 hours of use 6 Clean cab
39. strument is off flip the circuit breaker at the right side of the instrument on Do not turn power off except during maintenance and in case of an emergency The instrument will be in standby mode when not in use Press any key and the instrument becomes fully operational To end a run for any reason press STOP Power must be turned on and the vacuum system turned off to open chamber door Standard Operation 1 Use only the Beckman rotors and accessories designed for use in this instrument 2 See manual for specific instructions for filling sealing and capping tubes and bottles 3 Inspect tubes and bottles for cracks or flaws prior to use Do not use tubes and bottles if damaged 4 Donot overfill tubes that require plugs overfilling can cause overflow when plug is inserted however too much air can cause the tube to deform and disrupt gradients and sample bands as well as increasing the force required to remove the tube from the cavity after centrifugation 5 Unless otherwise specified the minimum recommended volume for bottles is half full this will reduce rotor speed for optimum performance Maximum and minimum fill levels and cap requirements are shown in table 3 6 of the manual 6 Ensure samples are symmetrically placed in rotor 7 With power on open the chamber door install the rotor and close and lock the chamber door Press VACUUM to evacuate the chamber 8 Enter the required run conditions Speed Temp and Time Hold o
40. tered inside the centrifuge should be done 5 Rotors and sample buckets should be cleaned and disinfected after each use with a non corrosive cleaning solution mild detergent and distilled water are recommended Metal test tube brushes must not be used for cleaning the inside of the buckets All traces of detergent should be removed prior to air drying 6 Cleaning of the inside of the centrifuge bucket with 70 isopropyl alcohol is recommended after every 30 hours of use 7 A regular inspection of the unit should be performed after 100 hours of use to inspect the rotor especially the inside cavities for rough spots cracks pitting white powder or deposits on aluminum rotors If any of these signs are evident do not run the rotor and call the manufacturer for servicing 29 8 Sample tubes bottles adapters and spacers should always be washed by hand using a mild detergent with brushes that will not scratch the tubes 9 Do not interchange tube cap components They are designed as a unit for a particular tube being centrifuged in a particular rotor 10 Centrifuges can suffer from rotor failure and other mechanical hazards as such they can be compromised by corrosion or fatigue Keep logs of maintenance and use for centrifuges 30 Eppendorf 5415 Biofuge A and other Low speed centrifuges Standard Operation D OT RION gt N Turn the power switch on Ensure the sample tubes are of equal weight and symmetrically placed in th
41. the lab supervisor notify Department of University Safety refer to hazardous agent use protocol if required refer to radiation safety guide for radiochemical spills if required General Safety Measures Centrifuges are instruments with strong potential for harming users due to the high speed at which they operate mechanical failure of the rotor can result in injury even death and sample container breakage can generate aerosols that are harmful to inhale Thus it is very important to act safely when using and maintaining these instruments FE IF AT ANY TIME YOU ARE UNSURE HOW TO OPERATE THE INSTRUMENT SAFELY PLEASE REFER TO THE INSTRUCTION MANUAL FOR DETAILS AND OR ASK THE LABORATORY SUPERVISOR OR DESIGNATED EMPLOYEE FOR ASSISTANCE 1 The centrifuge should always be installed according to the manufacturer specifications e Do not locate the instrument near areas containing flammable reagents or combustible fluids or where vibration will cause items to fall off nearby shelves e The centrifuge should be securely anchored by strong suction cups benchtop models wheel brakes floor models etc Movement of the instrument can damage parts and injure users e Request instructions from the manufacturer on safe transportation procedures if the centrifuge must be moved to another location and instructions are not in the operation manual 2 Proper selection use and maintenance of rotors is critical to safe operation Lack of care c
42. to or squeeze the tubes Tube contents will splash out when the plug or cap is removed if pressure is applied to the tube In case of a power failure the power should always be turned off and the instrument disconnected from the main power source In case of a power failure lasting for several hours the samples must be retrieved from the rotor Do not open until the rotor has stopped To open the lid manually a special unlocking tool is required If possible refer maintenance to qualified service personnel Care and Maintenance 1 Check regularly that the motor and brake circuits are working properly 2 Wash rotor components immediately if salts or other corrosive materials are used or spillage has occurred Do not allow corrosive materials to dry on the rotor 3 Rotors should be washed after every use in warm water containing a few drops of mild detergent 4 Ensure core of rotor which comes in contact with drive spindle is washed 5 Each cup or cavity of the rotor must be washed thoroughly using a small nylon brush do not use metal brushes All traces of detergent should be removed prior to air drying 6 Once the rotor is clean rinse with water distilled water is preferable 7 Dry rotor with a soft absorbent non woven cloth or tissue Drying may be finished off with warm air jet 8 For swing out rotors be sure to clean the grease from the trunnions and replace it with a small amount of fresh grease This will ensure that the bu
43. trument while it is operating Press ENTER DISPLAY and START the vacuum system is automatically activated To end run press STOP Do not switch off to stop run At end of run do not open chamber until the rotor comes to rest After rotor has stopped press VACUUM to vent chamber When working with infectious materials wait 10 minutes after rotor has stopped before opening the centrifuge lid Be aware of decontamination and clean up procedures that apply to materials being centrifuged If a breakage has occurred wait at least 10 minutes after rotor has stopped for aerosols and droplets to settle before opening lid Open chamber door and remove the rotor Ensure rotor chamber is clean and dry keep door closed between runs Centrifugation causes a slight vacuum to build up in the tube cavity occasionally resulting in the suction effect when removing the tubes from the rotor Allow at least 5 minutes after the rotor comes to rest to remove the tubes from the rotor Do not try to remove tubes by pulling the plugs Do not hold onto or squeeze the tubes Tube contents will splash out when the plug is removed if pressure is applied to the tube In case of a power failure turn off the instrument and disconnect it from the main power source 12 In case of a power failure lasting for several hours the samples must be retrieved from the rotor The control head cover and the front panel must be removed to disengage the door lock If possible
44. y taken out of service A service representative from the centrifuge manufacturer must be contacted and the unit completely inspected and parts requiring replacement ordered and replaced by the service representative Proper records must be kept thereafter Records and Responsibility University Safety It is the responsibility of the Department of University Safety to keep an up to date list of departments who have centrifuges in use The list is to be made available to the Ministry of Labour inspector on request Departmental Safety Officer It is the responsibility of the Departmental Safety Officer DSO of a department that uses centrifuge s to retain and keep up to date a list of all centrifuges in the department the list will include the name of the unit manufacturer serial building and room warranty and service information name of the person responsible for the unit ensure that the laboratory supervisor of any laboratory where a new instrument is installed is made aware of his her responsibilities as outlined in this document arrange for any assistance necessary to this supervisor regarding preparation of safety instructions development of training package and preparation of centrifuge use log and maintenance log ensure that the list of centrifuges in use is made available to the Department of University Safety or Ministry of Labour inspector on request Lab Supervisor It is the responsibility of the lab supervisor to
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