JA-30.50 Ti Fixed Angle Rotor
Contents
1. 0 0 0 0 000000 see Table 3 min Nominal tube dimensions 0 0005 25 x 104 mm i Nominal tube capacity 0 2 2 eee eee eee 50 mL Nominal rotor capacity 6 0 02 400 mL dogs RAS Approximate acceleration time to maximum speed rotor fully loaded in an Avanti J 30 I centrifuge to 30 000 rpm 5 4 min in a J2 series centrifuge to 20 000 rpm 4 min Approximate deceleration time from maximum speed rotor fully loaded max brake in an Avanti J 30 I centrifuge from 30 000 rpm 3 1 2 min in a J2 series centrifuge from 20 000 rpm 3 min Rotor weight empty 0 0 eee eee eee eee 11 2 kg 24 7 Ib Rotor weight maximum sample mass 11 8 kg 26 Ib Rotor material 0 0 0 2 cee eee eee ee titanium body aluminum lid Rotor entry code for microprocessor controlled J2 series centrifuges soe 0 00 n ecrit esii ee sede reece 30 5 At speeds above 20 000 rpm the minimum temperature set point will be above 4 C t The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass Passing through the critical speed range is characterized by some vibration Relative Centrifugal Field RCF is the ratio of the centrifugal acceleration at a specified radius and speed r to the standard acceleration of gravity g according to the following formula RCF 22. 979 King s Road Taikoo Place Hong Kong Telephone 852 2814 7431 2814 0481 Fax 852 2873 4511 ITALY Beckman Coulter S p a Centro Direzionale Lombardo Palazzo F 1 Via Roma 108 20060 Cassina de Pecchi Milano Italy Telephone 02 953921 Fax 02 95392264 JAPAN Beckman Coulter K K TOC Ariake West Tower 2 5 7 Ariake Koto ku Tokyo 135 0063 Japan Telephone 03 5404 8359 Fax 03 5404 8436 MEXICO Beckman Coulter de Mexico S A DE C V Avenida Popocat petl 396 Colonia Gral Pedro Maria Anaya Codigo Postal 03340 Mexico D F Mexico Telephone 52 55 9183 2800 NETHERLANDS Beckman Coulter Nederland B V Nijverheidsweg 21 3641 RP Mijdrecht Postbus 47 3640 AA Mijdrecht The Netherlands Telephone 0297 230630 Fax 0297 288082 SINGAPORE Beckman Coulter Singapore Pte Ltd 116 Changi Road Unit 03 01 02 Singapore 419718 Telephone 65 6339 3633 Fax 65 6336 6303 SOUTH AFRICA SUB SAHARAN AFRICA Beckman Coulter Stand 1A Primegro Park Tonetti Street 1685 Halfway House Johannesburg Republic of South Africa Telephone 27 11 805 2014 5 Fax 27 11 805 4120 e mail beckman intekom co za Internet www beckmancoulter com Telex 678413 2007 Beckman Coulter Inc SPAIN Beckman Coulter Espa a S A C Caleruega 81 28033 Madrid Spain Telephone 91 3836080 Fax 91 3836096 email bioresearch espana beckman com SWEDEN Beckman Coulter AB Archimedesvaegen 7 Box 1
3. the liquid is kept inside the rotor even if all eight tubes leak at the same time This feature helps to eliminate the escape of liquid into the centrifuge chamber O Ring Finger Grip Sealing Surface Grooves Fluid Containment Annulus rH al Figure 1 Fluid Containment Annulus JA 30 50 Ti Rotor The rotor body is made of titanium and the lid is made of anodized aluminum Two O rings made of Buna N rubber both located in the rotor lid help to maintain atmospheric pressure inside the rotor during centrifugation when they are properly lubricated Two horizontal pins in the rotor drive hole mate with the centrifuge drive hub to prevent the rotor from slipping during acceleration and deceleration For warranty information see the WARRANTY at the back of this manual PREPARATION AND USE PRERUN SAFETY CHECKS N Specific information about the JA 30 50 Ti rotor is given here Infor mation common to this and other rotors is contained in the manual Rotors and Tubes for J Series Centrifuges publication JR IM which should be used together with this manual for complete rotor and accessory information I gt NOTE Although rotor components and accessories made by other manufacturers may fit in the JA 30 50 Ti rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accesso ries in the JA 30 50 Ti rotor may void the rotor warranty and should be prohibited by
4. your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Read the Safety Notice page at the front of this manual before using the rotor 1 Make sure that the rotor and lid are clean and show no signs of corrosion or cracking 2 Check the chemical compatibilities of all materials used Refer to Appendix A in Rotors and Tubes 3 Verify that the tubes being used are listed in Table 3 JA 30 50 Ti Rotor ROTOR PREPARATION OPERATION For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration 1 Be sure that metal threads in the rotor are clean and lightly but evenly lubricated with Spinkote lubricant 306812 Also ensure that O rings are lightly but evenly coated with silicone vacuum grease 335148 Load the filled containers symmetrically into the rotor Refer to TUBES AND BOTTLES on page 11 for information about containers If fewer than eight tubes are being run they must be arranged symmetrically in the rotor see Figure 2 Opposing tubes must be filled to the same level with liquid of the same density Figure 2 Typical Examples of Arranging Tubes in the Rotor Two four six or eight tubes can be centrifuged per run if they are arranged in the rotor as shown gt NOTE Do not run an empty rotor Place filled tubes in at least two opposing cavities e For low temperature runs pr
5. 11 56 SE 168 11 Bromma Sweden Telephone 0 8 564 85 900 Telefax 0 8 564 85 901 SWITZERLAND Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 0800 850 810 Fax 0848 850 810 TAIWAN Beckman Coulter Taiwan Inc Taiwan Branch 8th Floor 216 Tun Hwa South Road Section 2 Taipei 106 Taiwan Republic of China Telephone 886 2 2378 3456 Fax 886 2 2377 0408 TURKEY Beckman Coulter Ltd E 5 Yanyol Faith Cad 81410 Soganlik Kartal Istanbul Turkey Telephone 90 216 309 1900 Fax 90 216 309 0090 UNITED KINGDOM Beckman Coulter United Kingdom Ltd Oakley Court Kingsmead Business Park London Road High Wycombe Buckinghamshire HP11 1JU England U K Telephone 01494 441181 Fax 01494 447558 e mail beckman_uk beckman com Authorized dealers in other countries 4300 N Harbor Boulevard Box 3100 Fullerton California 92834 3100 e Fax 1 800 643 4366 Lry QO Printed on recycled paper
6. 2 ee eee eee 346965 Silicone vacuum grease 1 OZ 20 eee eee ee eee 335148 Beckman Solution 555 1 qt 2 2 0 cece ee eee 339555 Spinkote lubricant 2 02 00 eee cee eee 306812 J SERIES ROTOR WARRANTY Subject to the conditions specified below and the warranty clause of the Beckman Coulter Inc terms and conditions of sale in effect at the time of sale Beckman Coulter Inc agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within seven 7 years after delivery of a J series rotor to the original buyer by Beckman Coulter Inc or by an authorized represen tative provided that investigation and factory inspection by Beckman Coulter Inc discloses that such defect developed under normal and proper use Should a Beckman Coulter cen trifuge be damaged due to a failure of a rotor covered by this warranty Beckman Coulter will supply free of charge all cen trifuge parts required for repair REPLACEMENT Any product claimed to be defective must if requested by Beckman Coulter Inc be returned to the factory transporta tion charges prepaid and will be returned to Buyer with the transportation charges collect unless the product is found to be defective in which case Beckman Coulter Inc will pay all transportation charges A defective rotor will be replaced by Beckman Coulter Inc at its then current list price less a credit based upon the age of
7. 700 47 700 25 800 22 000 58 500 40 100 21 700 20 000 48 400 33 200 17 900 18 000 39 200 26 900 14 500 16 000 31 000 21 200 11 500 14 000 23 700 16 200 8 780 12 000 17 400 11 900 6 450 10 000 12 100 8 290 4 480 8 000 7 740 5 300 2 870 6 000 4 360 2 980 1610 4 000 1940 1330 717 2 000 484 331 179 108 860 is the calculated Fmax value with no rounding applied RCF xg 120 000 100 000 80 000 60 000 40 000 20 000 0 Relative Centrifugal Fields for the JA 30 50 Ti Rotor ii i 2500 5000 10000 12500 Speed rpm 20 000 25000 27500 30 000 JA 30 50 Ti Rotor 16 CARE AND MAINTENANCE MAINTENANCE I gt NOTE Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use Ye Periodically at least monthly inspect the rotor especially inside oe Ca cavities for rough spots or pitting or heavy discoloration If any 870612 Eg of these signs are evident do not run the rotor Contact your Small Beckman Coulter representative for information about the Field Check On Eh Rotor Inspection Program and the rotor repair center for Corrosion Regularly lubricate the metal threads in the rotor with a thin even Rotor coat of Spinkote lubricant Failure to keep these threads lubricated Bo
8. 80 29 x 104 mm thickwall polycarbonate 363647 35 none 30 000 rpm 50 mL no cap pkg 25 108 800 x g 280 29 x 104mm thickwall polypropylene 357007 35 none 30 000 rpm 50 mL no cap pkg 25 108 800 x g 280 29 x 104 mm polycarbonate bottle 357002 40 none 25 000 rpm 50 mL screw cap 75 600 x g pkg 25 403 29 x 104 mm polyallomer bottle 357003 40 none 25 000 rpm 50 mL screw cap 75 600 x g pkg 25 403 29 x 104 mm thickwall polycarbonate 363664 36 5 none 25 000 rpm 50 mL snap on cap 75 600 x g pkg 25 403 29 x 104 mm thickwall polypropylene 357005 36 5 none 25 000 rpm 50 mL snap on cap 75 600 x g pkg 25 403 16 x 80 mm polycarbonate bottle 355672 10 adapter 361703 30 000 rpm 10 mL screw cap Ultem 91 000 xg pkg 25 244 11 x 38 mm polyallomer tube 357448 1 3 adapter 361705 30 000 rpm 1 5 mL snap on cap natural Ultem 73 400 x g pkg 500 126 11 x 38 mm polypropylene tube 356090 1 3 adapter 361705 30 000 rpm 1 5 mL snap on cap natural 73 400 x g pkg 500 126 25 x 104 mm glass tube ae FE adapter 870331 10 000 rom 50 mL rubber 12 100 xg 494 Maximum speeds listed are guidelines only These speeds have been achieved in reliability tests at Beckman Coulter but because of variances in user methodologies no guarantee of performance is expressed or implied At 2 C at 25 C maximum speed must be limited to 26 000 rpm for tube 357001 and to 29 000 rpm for tube 363647 Above 20 C fill polypropylen
9. COULTER 2007 Beckman Coulter Inc Printed in the U S A J TB 070H March 2007 JA 30 50 Ti Fixed Angle Rotor Used In Beckman Coulter J2 and Avanti J Series Centrifuges JA 30 50 Ti Rotor SAFETY NOTICE This safety notice summarizes information basic to the safe use of the rotor described in this manual The international symbol displayed above is a reminder to the user that all safety instructions should be read and understood before operation or maintenance of this equipment is attempted When you see the symbol on other pages throughout this publication pay special attention to the specific safety informa tion presented Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the rotor This rotor was developed manufactured and tested for safety and reliability as part of a Beckman Coulter centrifuge rotor system Its safety or reliability can not be assured if used in a centrifuge not of Beckman Coulter s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter s approval A e Pe ee ee ie Handle body fluids with care because they can transmit disease No known test offers complete assurance that they are free of micro organisms Some of the most virulent Hepatitis B and C and HIV I V viruses atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protec
10. Consult your laboratory safety officer regarding the proper decontamination methods to use If the rotor or other components are contaminated with toxic or patho genic materials follow all appropriate safety and decontamination procedures as outlined by your laboratory safety officer STERILIZATION AND DISINFECTION 121 C Qm STORAGE e The rotor and all rotor components can be autoclaved at 121 C for up to an hour Remove the lid from the rotor and place the rotor lid and spacers in the autoclave upside down Ethanol 70 or hydrogen peroxide 6 may be used on all rotor components including those made of plastic Bleach sodium hypochlorite may be used but may cause discoloration of anod ized surfaces Use the minimum immersion time for each solution per laboratory standards While Beckman Coulter has tested these methods and found that they do not damage the rotor or components no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfec tion is a concern consult your laboratory safety officer regarding proper methods to use Refer to publication IN 192 included in each box of tubes or bottles for tube and bottle sterilization and disinfection procedures When the rotor is not in use store it in a dry environment not in the instrument with the lid removed to allow air circulation so moisture will not collect in the tube cavities 3 Flammability hazard D
11. E where r is the radius in millimeters is the angular velocity in radians per second 2 RPM 60 and g is the standard acceleration of gravity 9807 mm s After substitution m REM RCF 1 127 REM JA 30 50 Ti Rotor DESCRIPTION Lid z Small O Ring e i 011757 D Large O Ring 870612 N Rotor Body This rotor has been manufactured in a registered ISO 9001 or 9002 facility for use with the specified Beckman Coulter centrifuges The JA 30 50 Ti fixed angle rotor rated for 30 000 rpm is designed to hold up to eight 50 mL tubes at a 34 degree angle Used in Beckman Coulter Avanti J and J2 series centrifuges the JA 30 50 Ti rotor develops centrifugal forces that can efficiently pellet cells from large volumes or cell particles from tissue homogenates Short column methods i e partially filled tubes may also be used to purify large quantities of virus in a cushion gradient An available dual locking lid mechanism allows the rotor to be loaded into and removed from the centrifuge with the lid in place The rotor may be placed under a safety hood before the lid is attached or removed If biosafety is not a concern a single locking lid mecha nism is also available The JA 30 50 Ti rotor has a patented fluid containment annulus located below the O ring sealing surface see Figure 1 If tubes are overfilled or if leakage occurs during centrifugation the annulus holds enough volume that all of
12. Press the key to enter a minus sign pressing it again will remove the minus sign JA 30 50 Ti Rotor 5 Check the temperature display If the entry is incorrect press CE and reenter the digits 6 When the entry is correct press ENTER RECALL gt NOTE To clear a COMP ADJ entry press COMP ADJ 0 and ENTER RECALL REMOVAL AND SAMPLE RECOVERY A CAUTION If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate safety and decontamination procedures to the centrifuge and accessories as required Dual locking Lid 1 Place one hand on the rotor to keep it from turning Grasp the tie down knob with your other hand and turn it to the left counter clockwise until the stem disengages from the drive hub threads 2 Hold the tie down knob or place your hands on the rotor and lift the rotor straight up off the drive hub 3 Place the rotor on a counter or bench top under a safety hood 4 Grasp the daisy knob and turn it to the left counterclockwise until it disengages 5 Remove the lid and set it aside 10 TUBES AND BOTTLES N a o O O p JA 30 50 Ti Rotor Single locking Lid 1 Unscrew the tie down knob 2 Remove the lid and set it aside 3 Lift the rotor straight up off the drive hub The JA 30 50 Ti rotor uses the tubes and bottles listed in Table 3 Be sure to use only those items listed and to observe the
13. TENANCE OR REPLACEMENT OF THE PRODUCT Beckman Coulter Worldwide Biomedical Research Division Offices AUSTRALIA Beckman Coulter Australia Pty Ltd Unit D 24 College St Gladesville NSW 2111 Australia Telephone 61 2 9844 6000 or toll free 1 800 060 880 Fax 61 2 9844 6096 email lifescienceaustralia beckman com CANADA Beckman Coulter Canada Inc 6755 Mississauga Road Suite 600 Mississauga Ontario Canada L5N 7Y2 Telephone 905 819 1234 Fax 905 819 1485 CHINA Beckman Coulter Inc Beijing Representative Office Unit 2005A 2006 2009 East Ocean Center Jian Guomenwai Avenue Beijing 100004 China Telephone 86 10 6515 6028 Fax 86 10 6515 6025 6515 6026 EASTERN EUROPE MIDDLE EAST NORTH AFRICA Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 22 994 07 07 Fax 41 22 994 07 00 FRANCE Beckman Coulter France S A Paris Nord II 33 66 rue des Vanesses B P 50359 Villepinte France 95942 ROISSY CDG Cedex Telephone 01 49 90 90 00 Fax 01 49 90 90 10 e mail bioresearch france beckman com GERMANY Beckman Coulter GmbH Europark Fichtenhain B 13 47807 Krefeld Germany Telephone 49 21 513335 Fax 49 21 51333633 e mail bioresearch de beckman com Ozons COULTER Beckman Coulter Inc e Sales and Service 1 800 742 2345 HONG KONG Beckman Coulter Hong Kong Ltd 12th Floor Oxford House
14. age you should assume that some fluid escaped the rotor Apply all appropriate safety and decontamination procedures to the centrifuge and accessories as required Never exceed the maximum rated speed of the rotor and labware in use Refer to the section on RUN SPEEDS Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use JA 30 50 Ti Rotor JA 30 50 Ti FIXED ANGLE ROTOR SPECIFICATIONS Maximum speed in Avanti J 30I centrifuges 0 00005 30 000 rpm in Avanti J 26XP series centrifuges 26 000 rpm in Avanti J 25 series centrifuges 25 000 rpm Critical speed range ononon ononon unur eee 600 to 800 rpm L_ Maximum solution density 0 00 0 cee eee eee 1 2 g mL 4 Relative Centrifugal Field at maximum speed a al f zmar LOS MM eoriet ok eee ee edd Meee EES ate 108 860 x g Z at ray 74 MM 42 265 eke aoaaa araara 74 600 x g at rimm AQ MM 2 oe ig cpaienetok stad nines o 40 320 x g k factor at maximum speed 0 00 280 Conditions requiring speed reduction see RUN SPEEDS Maximum allowable imbalance of opposing loads 2 grams Number of tube cavities round bottom 000000 e eee 8 l Available bottles and tubes
15. at the rotor cannot achieve the required temperature at this speed SET COMP TEMPERATURE C OTO JA 30 50 Ti Rotor Table 2 Temperature Compensation Settings for the Model J2 HC Analog Centrifuge Interpolate if intermediate values are required Rotor Required Sample Temperature C Speed rpm 20 10 2 5 10 20 40 18 000 N 5 5 5 3 3 3 15 000 4 3 4 3 3 3 1 10 000 3 2 1 2 1 0 1 An N indicates that the rotor cannot achieve the required temperature at this speed Analog J2 Series Centrifuges Models J2 HS and J2 HC Turn the SET knob on the centrifuge control panel to the required sample temperature Find the compensation value in Table 1 for Model J2 HS or Table 2 for Model J2 HC that corresponds to the required temper ature and run speed Set the COMP dial to that setting Interpolate if intermediate values are required Microprocessor Controlled J2 Series Centrifuges Models J2 MI and J2 MC Enter rotor code 30 5 then follow the steps below 1 Press the TEMP key on the centrifuge control panel and then use the keypad to enter the sample temperature Find the compensation value in Table 1 that corresponds with the set temperature and run speed Press COMP ADJ The word COMP flashes below the TEMPERATURE display and the display flashes Use the keypad to enter the required compensation value
16. dy can result in damaged threads Regularly apply silicone vacuum grease to the O rings Replace O rings about twice a year or whenever worn or damaged Refer to Appendix A in Rotors and Tubes for chemical compatibili ties of rotor and accessory materials Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center CLEANING Rotor Cleaning Kit 339558 Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred Do not allow corrosive materials to dry on the rotor Under normal use wash the rotor frequently at least weekly to prevent buildup of residues Remove the O rings before washing Wash the rotor and lid in a mild detergent such as Beckman Solution 555 that won t damage the rotor The Rotor Cleaning Kit 339558 contains two special plastic coated brushes and two quarts of Solution 555 for use with rotors and accessories Dilute the detergent 10 to 1 with water JA 30 50 Ti Rotor IlIi NOTE Do not wash rotor components in a dishwasher Do not soak in detergent solution for long periods such as overnight 3 Thoroughly rinse the cleaned rotor and components with distilled water aR 4 Air dry the rotor and lid upside down Do not use acetone to dry Small O Ring 011757 the rotor x Large O Ring 870612 5 Apply a thin even coat of silicone vacuum grease to both lid O r
17. e tubes at least half full Containers may be filled less than or equal to the maximum fill volumes provided in this table k r e i Z Observe manufacturer s maximum speed and fill guidelines Ultem is a registered trademark of GE Plastics JA 30 50 Ti Rotor Polycarbonate and Polyallomer Bottles Capped polycarbonate and polyallomer bottles may be centrifuged completely filled or partially filled not less than half full Containers placed in opposing positions in the rotor must be filled to the same level Gece 4 RUN TIMES The k factor of the rotor is a measure of the rotor s pelleting TIME HR MIN efficiency Beckman Coulter has calculated the k factors for all of its rotors at maximum speed with full tubes using the following 7 m formula In 7 nax min 10 1 o2 3600 where wis the angular velocity of the rotor in radians per second 0 105 x rpm Pax is the maximum radius and r 1s the minimum radius After substitution rpm 2 Use the k factor in the following equation to estimate the run time t in hours required to pellet particles of known sedimentation coeffi cient s in Svedberg units S 13 JA 30 50 Ti Rotor Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor as follows 2 E 30000__ o aa run speed 4 Run times can also be estimated from data established in prior experi ments using a different r
18. ecool the rotor in the centrifuge or in a refrigerator before use especially before short runs to ensure that the rotor reaches the set temperature A suggested precooling cycle is a minimum of 30 minutes at 2000 rpm at the required temperature INSTALLING THE ROTOR JA 30 50 Ti Rotor If fluid containment is required use capped tubes or bottles It is strongly recommended that all containers carrying physiological fluids be capped to prevent leakage If you are using an Avanti J series centrifuge select the JA 30 50 Ti rotor If you are using a microprocessor controlled J2 series centrifuge be sure to enter rotor code 30 5 Refer to your centrifuge instruction manual for additional information To prevent the rotor from sticking to the centrifuge drive hub apply a thin coat of Spinkote lubricant to the rotor drive pins and to the lid knob threads Lightly coat the large and small lid O rings with silicone vacuum grease Dual locking lid only Place filled tubes into the rotor then place the lid on the rotor and turn the daisy knob to the right clockwise until secure Do not overtighten Carefully lower the rotor straight down onto the centrifuge drive spindle hub Do not drop the rotor onto the spindle hub Slowly turn the rotor around the drive spindle to make sure that it is properly seated Secure the rotor to the centrifuge drive spindle as follows Dual locking lid press the lid knob do
19. f the items without review of the reported problem Use the address label printed on the RGA form when mailing the rotor and or accessories to Beckman Coulter Inc 1050 Page Mill Road Palo Alto CA 94304 Attention Returned Goods Customers located outside the United States should contact their local Beckman Coulter office 19 JA 30 50 Ti Rotor 20 SUPPLY LIST For detailed information on ordering parts and supplies call Beckman Coulter Sales 1 800 742 2345 in the United States or your local Beckman Coulter office or see the Beckman Coulter High Perfor mance High Speed High Capacity Rotors Tubes amp Accessories cata log BR 8102 available at www beckmancoulter com For your convenience a partial list is given below REPLACEMENT ROTOR PARTS SUPPLIES Rotor assembly dual locking lid 0 0 e 363420 Rotor assembly single locking lid 0 04 363421 Lid assembly dual locking 0 0 0 0 00 e eee eee eee 363424 Lid assembly single locking 0 0 0 0 0 eee eee ee 363430 Tie down assembly single locking lid 0 363431 Tie down assembly dual locking lid 000 363426 Large lid O 1n8 24 vieve eevee ee bane E E bans 870612 Small lid OFig sesse noke eee eee eee 011757 Tubes bottles and adapters 0 e eee ee eee ee see Table 3 Rotor Cleaning Kit 2 0 00 0 ee eee ee eee 339558 Rotor removal tool 0
20. ings before replacing them in the lid Clean metal threads every 6 months or as necessary Use a brush and concentrated Solution 555 Rinse and dry thoroughly then lubricate lightly but evenly with Spinkote to coat all threads Periodically remove the O rings and wipe clean as necessary Clean the O ring grooves with a cotton tipped swab Reapply a light film of silicone vacuum grease DECONTAMINATION If the rotor and or accessories becomes contaminated with radioac tive material it should be decontaminated using a solution that will not damage the anodized surfaces Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum RadCon Surface Spray or IsoClean Solution for soaking and Radiacwash IlI NOTE IsoClean can cause fading of colored anodized surfaces Use it only when necessary and remove it promptly from surfaces 1 In U S contact Nuclear Associates New York in Eastern Europe and Commonwealth States contact Victoreen GmbH Munich in South Pacific contact Gammasonics Pty Ltd Australia in Japan contact Toyo Medic Co Ltd Tokyo 2 In U S contact Biodex Medical Systems Shirley New York internationally contact the U S office to find the dealer closest to you 17 JA 30 50 Ti Rotor 18 While Beckman Coulter has tested these methods and found that they do not damage components no guarantee of decontamination is expressed or implied
21. maximum speed limits and fill volumes shown Maximum fill volume is the maximum amount that can be centrifuged in the container listed Refer to Appendix A in Rotors and Tubes for information on the chemical resistances of tube bottle and accessory materials Temperature Limits e Plastic containers have been tested for use at temperatures between 2 and 25 C For centrifugation at other temperatures pretest containers under anticipated run conditions e If plastic containers are frozen before use make sure that they are thawed to at least 2 C prior to centrifugation Thickwall Tubes Thickwall polyallomer polypropylene and polycarbonate tubes can be run partially filled at least half filled with or without caps but all opposing tubes for a run must be filled to the same level with liquid of the same density Do not overfill capless tubes 11 JA 30 50 Ti Rotor 12 Table 3 Available Tubes and Bottles for the JA 30 50 Ti Rotor Use only the items listed here and observe fill volumes and maximum run speeds Tube or Bottle Required Accessory Max Fill Max Speed Dimensions Part Volume Part RCF and Volume Description Number mL Description Number k Factor 29 x 104 mm polycarbonate bottle 357000 40 none 30 000 rpm 50 mL w cap assy 108 800 x g pkg 6 280 29 x 104 mm polyallomer bottle 357001 40 none 30 000 rpm 50 mL w cap assembly 108 800 x g pkg 6 2
22. o not use in or near operating centrifuges JA 30 50 Ti Rotor RETURNING A ROTOR Before returning a rotor or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from RGA Beckman Coulter Inc This RGA form which may be obtained from a your local Beckman Coulter sales office should contain the following information e rotor serial number e history of use approximate frequency of use e reason for the return e original purchase order number billing number and shipping number if possible e name and phone number of the person to be notified upon receipt of the rotor or accessory at the factory and e name and phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that the parts are free from pathogens and or radioactivity Steriliza tion and decontamination must be done before returning the parts Smaller items such as tubes bottles etc should be enclosed in a sealed plastic bag All parts must be accompanied by a note plainly visible on the out side of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal o
23. otor if the k factor of the previous rotor is known For any two rotors a and b ta k e 5 ty b where the k factors have been adjusted for the actual run speed used For more information on k factors see Use of k Factor for Estimating Run Times from Previously Established Run Conditions publication DS 719 RUN SPEEDS SPEED RPM RCF The centrifugal force at a given radius in a rotor is a function of run speed Comparisons of forces between different rotors are made Ju tt 1G RAT by comparing the rotors relative centrifugal fields RCF When rotational speed is selected so that identical samples are subjected to the same RCF in two different rotors the samples are subjected to the same force see Table 4 Do not select run speeds in excess of 30 000 rpm When solutions more dense than 1 2 g mL are centrifuged in this rotor use equation 6 to calculate the reduced maximum allowable rotor speed 1 2 g mL 6 reduced maximum speed 30 000 lt density of tube contents 14 JA 30 50 Ti Rotor Table 4 Relative Centrifugal Fields for the JA 30 50 Ti Rotor Entries in this table are calculated from the formula RCF 1 12 r RPM 1000 2 and then rounded to three significant digits Relative Centrifugal Field x g Rotor Speed At Imax At fay At fmin rpm 108 mm 74 mm 40 mm 30 000 108 860 74 600 40 300 28 000 94 800 65 000 35 100 26 000 81 800 56 000 30 300 24 000 69
24. the rotor years since date of purchase The Buyer shall not receive credit until the claimed defective rotor is returned to Beckman Coulter s Palo Alto California facility or delivered to a Beckman Field Service representative The replacement price cost to Buyer for the respective rotor shall be calculated as follows Replacement price Current rotor list price x years 7 CONDITIONS 1 Except as otherwise specifically provided herein this war ranty covers the rotor only and Beckman Coulter Inc shall not be liable for damage to accessories or ancillary supplies including but not limited to i tubes ii tube caps iii tube adapters or iv tube contents 2 This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance con trary to the instructions in the Beckman Coulter rotor or centrifuge manual 3 This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge unmatched to the rotor charac teristics or is operated in a Beckman Coulter centrifuge that has been improperly disassembled repaired or modified DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WAR RANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANT ABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CON SEQUENTIAL DAMAGES OF ANY KIND WHATSO EVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAIN
25. tion Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this rotor without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials in nor handle or store them near the centrifuge Although rotor components and accessories made by other manufacturers may fit in the JA 30 50 Ti rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the JA 30 50 Ti rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Do not run an empty rotor Place filled tubes in at least two opposing cavities Make sure that filled containers are loaded symmetrically into the rotor and that opposing tubes are filled to the same level with liquid of the same density If disassembly reveals evidence of leak
26. wn and turn it to the right clockwise until secure no more than two full turns Do not overtighten Single locking lid place filled tubes into the rotor then place the lid on the rotor Press the lid knob down and turn it to the right clockwise until secure do not overtighten A WARNING If the rotor is left in the centrifuge between runs make sure that the rotor is seated on the drive hub and that the tie down knob is tight before each run JA 30 50 Ti Rotor TEMPERATURE TEMP C To ensure that the JA 30 50 Ti rotor reaches the required temperature during the run follow the appropriate instructions below for the centrifuge model being used Avanti J Series Centrifuges Enter the run temperature according to the instructions in your centrifuge instruction manual No additional input is required J2 Series Centrifuges Enter the required run temperature and the appropriate temperature compensation units see Tables 1 and 2 on the centrifuge control panel as follows Table 1 Temperature Compensation Settings for Analog and Microprocessor Controlled Centrifuges Models J2 HS J2 M1 and J2 MC Interpolate if intermediate values are required Rotor Required Sample Temperature C Speed rpm 20 10 2 5 10 20 40 20 000 N N 6 6 6 4 9 18 000 N 6 4 6 5 4 8 15 000 3 5 3 5 5 3 7 10 000 2 2 2 1 2 2 6 An N indicates th
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