Agilent 1260 Infinity Manual Injector
Contents
1. d rd 49 I CSS 9 Figure 4 Installing the System 1260 Infinity MI User Manual 11 2 Installing the Manual Injector APG remote connector Start cable Position sensor cable DDDODDD Figure 5 Installing the Start Cable 1 See Figure 6 on page 14 6 Connect the capillaries to the manual injector see Flow Connections on page 13 12 1260 Infinity MI User Manual Installing the Manual Injector 2 Flow Connections WARNING Toxic and hazardous solvents and flammable liquids The handling of solvents and reagents can hold health risks gt When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents and flammable liquids are used CAUTION Prevent siphoning The outlets of the two vent capillaries ports 5 and 6 and the needle port must be at the same level to prevent siphoning see Figure 7 on page 14 1 Connect the pump outlet capillary to port 2 2 Connect the column compartment inlet capillary to port 3 3 Connect the sample loop between ports 1 and 42. Em Agilent Technologies Notices Agilent Technologies Inc 2006 2008 2010 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61328 90012 Edition 06 2010 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate
3. the sample loop At least 2 to 3 sample loop volumes more if better precision is required of sample should be injected through the needle port to provide good precision The sample fills the loop and excess sample is expelled through the vent tube connected to port 6 from pump Figure 9 LOAD Position 1260 Infinity MI User Manual 21 3 Using the Manual Injector INJECT Position In the INJECT position see Figure 10 on page 22 the pump is connected to the sample loop ports 1 and 2 connected All of the sample is washed out of the loop onto the column The needle port is connected to the vent tube port 5 Needle port to column from pump Sample loop Figure 10 INJECT Position 22 1260 Infinity MI User Manual 1260 Infinity MI User Manual e e o ee 4 d Maintenance e Overview of Maintenance 24 Flushing the Manual Injector 25 Cleaning the Manual Injector 26 Stator Face 27 Injection Valve Seal 29 Position Sensing Switch 32 Instructions on simple routine repair procedures OE Agilent Technologies 23 4 Maintenance Overview of Maintenance Table2 Overview of Repair Procedures Procedure Typical Frequency Time Required Notes Flushing the injector Exchanging the stator face Exchanging the injection valve seal Exchanging the position sensing switch After using aqueous buffers or salt solutions When visibly scratched or when the valve performance shows indicati
4. 1260 Infinity MI User Manual 13 2 Installing the Manual Injector Flow Lonnections 4 Connect one vent capillary supplied with valve to port 5 and one to port 6 N Y Sample loop Vent tube To column From pump outlet pwn Figure 6 Flow Connections EJ Vent capillaries ports 5 and 6 e MMM Vent capillaries and needle port at the same level Figure 7 Vent Capillaries 14 1260 Infinity MI User Manual Installing the Manual Injector 2 Leak Drainage WARNING Leaking injector fittings In the event of a leak solvent will drop into the leak channel in the baseplate from where it is channelled to the front and back of the baseplate gt Check the manual injector fittings periodically for signs of leakage Leak channel Figure 8 Leak Drainage 1260 Infinity MI User Manual 15 2 Installing the Manual Injector Leak Drainage 16 1260 Infinity MI User Manual 1260 Infinity MI User Manual e e e eece 3 d Using the Manual Injector LJ Solvent Information 18 Choice of Injection Seal 19 Needles 20 Injecting Sample 21 How to use the manual injector E Agilent Technologies 3 Using the Manual Injector Solvent Information 18 Observ
5. 8 Damaged Packaging 8 Delivery Checklist 8 Installing the Manual Injector 9 Flow Connections 13 LeakDrainage 15 Installation of the manual injector n Agilent Technologies 2 Installing the Manual Injector Unpacking the Manual Injector Damaged Packaging Upon receipt of your manual injector inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged save them until the contents have been checked for completeness and the manual injector has been mechanically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carriers inspection Delivery Checklist Ensure all parts and materials have been delivered with the manual injector The delivery checklist is shown in Table 1 on page 8 To aid in parts identification please see Parts and Materials for Maintenance on page 35 Please report missing or damaged parts to your local Agilent Technologies sales and service office Table1 Manual Injector Checklist Description Quantity Manual injection valve p n 5063 6502 with start cable including operating 1 instructions needle port cleaner vent tubes x2 and fittings 5 64 and 9 64 inch hex keys Mounting pole p n 5001 3738 1 Connection capillary 0 17 mm id 500 mm p n G1328 87600 1 Base plate p n G1328 44111 1 Organizer plate p n 5042 8553 1 Catch tube cap p n 5042 8576 1 Valve syri
6. e the following recommendations on the use of solvents Flow Cell Long term operation at pH gt 11 should be avoided Never leave strongly alkaline solutions in the flow cell without flow Solvents Always filter solvents through 0 4 um filters small particles can permanently block filters frits and capillaries Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCI3 O2 2COCI2 2HCI This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol may attack Steel Mixtures of carbon tetrachloride with 2 propanol or THF dissolve stainless Steel 1260 Infinity MI Us
7. er Manual Using the Manual Injector 3 Choice of Injection Seal The manual injector is supplied with a Vespel injection seal as standard Vespel is sensitive to alkaline attack so avoid mobile phases with a pH of 10 or more Use the Tefzel injection seal see Injection Valve Assembly on page 38 for mobile phases with a pH above 10 1260 Infinity MI User Manual 19 3 Using the Manual Injector Needles CAUTION Needle can damage valve gt Always use the correct size needle The manual injector is not supplied with syringes or needles Use needles with 0 028 inch outer diameter 22 gauge x 2 inch long needle without electro taper and with 90 point style square tip 20 1260 Infinity MI User Manual Using the Manual Injector 3 Injecting Sample WARNING Ejection of mobile phase When using sample loops larger than 100 pl mobile phase may be ejected from the needle port as the mobile phase in the sample loop decompresses Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Needle port Waste to column Sample loop LOAD Position In the LOAD position see Figure 9 on page 21 the pump is connected directly to the column ports 2 and 3 connected and the needle port is connected to
8. ms 1 8 Item p n 1 0101 1254 2 1535 4046 3 0101 0623 0101 0620 0101 1255 4 0100 1859 5 0100 1860 6 1535 4857 7 8710 0060 8 0490 1849 Description Bearing ring order rebuild kit instead Isolation seal Rotor seal Vespel Rotor seal Tefzel Rotor seal PEEK Stator face Stator head Stator screws Hex key wrench 9 64 inch Position sensing switch 1260 Infinity MI User Manual Parts and Materials for Maintenance 5 Injection Valve Assembly Figure 13 Injection Valve Assembly 1260 Infinity MI User Manual 39 5 40 Parts and Materials for Maintenance Sample loops stainless steel p n Description 0101 1248 Sample loop 5 uL 0100 1923 Sample loop 10 uL 0100 1922 Sample loop 20 uL 0100 1924 Sample loop 50 uL 0100 1921 Sample loop 100 pL 0101 1247 Sample loop 200 pL 0101 1246 Sample loop 500 pL 0101 1245 Sample loop 1 mL 0101 1244 Sample loop 2 mL 0101 1243 Sample loop 5 mL Sample loops PEEK p n Description 0101 1241 Sample loop 5 uL 0101 1240 Sample loop 10 uL 0101 1239 Sample loop 20 uL 0101 1238 Sample loop 50 uL 0101 1242 Sample loop 100 pL 0101 1227 Sample loop 200 pL 0101 1236 Sample loop 500 pL 0101 1235 Sample loop 1 mL 0101 1234 Sample loop 2 mL 0101 1230 Sample loop 5 mL 1260 Infinity MI User Manual 1260 Infinity MI User Manual e 2 e ece 6 d Appendix J T Agilent Technologies on Internet Additional information 42 ORE Agilent Technologies 41 6 Ap
9. n about the Agilent 1260 Infinity Manual Injector The manual describes the following introduction to the manual injector installing the manual injector using the manual injector maintenance of the manual injector parts and materials and additional information O Agilent Technologies 2006 2008 2010 Printed in Germany 06 2010 G1328 90012 d Agilent Technologies
10. nge fixed needle 50 pL p n 5182 9619 1 User Manual p n G1328 90011 1 1260 Infinity MI User Manual Installing the Manual Injector 2 Installing the Manual Injector CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions The manual injector can be installed at the left or right hand side of the instrument stack 1 Place the baseplate on the bench 2 Connect the two organizer plates to the base plate Figure 2 Connecting the Organizer Plates 3 Screw the mounting pole into one of the two holes in the organizer plate 1260 Infinity MI User Manual 9 2 Installing the Manual Injector 4 Slide the manual injector onto the mounting pole see Figure 3 on page 10 Tighten the mounting screw Mounting screw Figure 3 Installing the Mounting Pole and Manual Injector 10 1260 Infinity MI User Manual Installing the Manual Injector 2 5 Install other system modules on top of the manual injector baseplate see Figure 4 on page 11 000 0000 0000 o o
11. njector 26 Stator Face 27 Injection Valve Seal 29 Position Sensing Switch 32 Parts and Materials for Maintenance 35 Manual Injector 36 Injection Valve Assembly 38 Appendix 41 Agilent Technologies on Internet 42 1260 Infinity MI User Manual 1260 Infinity MI User Manual e e ee 1 d Introduction e Introduction to the Manual Injector Operation and mechanical hardware 6 ORE Agilent Technologies Introduction to the Manual Injector Introduction to the Manual Injector See also the Rheodyne 7725i operating instructions supplied with the injection valve The Agilent 1260 Infinity Manual Injector uses a Rheodyne 77251 7 port sample injection valve Sample is loaded into the external 20 ul sample loop through the injection port at the front of the valve The valve has a ceramic stator and Vespel injection seal for pH above 10 a Tefzel seal is available A make before break passage in the stator ensures flow is not interrupted when the valve is switched between the INJECT and LOAD positions and back again see also Needles on page 20 and Flow Connections on page 13 The valve is mounted on a steel mounting pole and can be installed at the left or right hand side of the LC system Mounting pole Injection port Figure 1 Rheodyne 7725i Injection Valve 6 1260 Infinity MI User Manual 1260 Infinity MI User Manual 2 Installing the Manual Injector Unpacking the Manual Injector
12. on of leakage or wear After approximately 10000 to 20000 injections or when the valve performance shows indication of leakage or wear When cable damaged or when no start signal is sent when switching to the inject position 5 minutes 10 minutes 10 minutes 10 minutes See Flushing the Manual Injector on page 25 See Stator Face on page 27 See Injection Valve Seal on page 29 See Position Sensing Switch on page 32 24 1260 Infinity MI User Manual Maintenance 4 Flushing the Manual Injector CAUTION Damage through crystal formation The use of aqueous buffers or salt solutions can lead to crystal formation which may cause scratches on the injection seal Always rinse the valve with water after using aqueous buffers or salt solutions 1 Switch the valve to the INJECT position 2 Use the pump to flush the sample loop and seal grooves 3 Use the needle port cleaner supplied with the valve and syringe to flush the needle port and vent capillary Needle port Syringe Needle port cleaner Figure 11 Needle port Cleaner 1260 Infinity MI User Manual 25 4 Maintenance Cleaning the Manual Injector The manual injector base should be kept clean Cleaning should be done witha soft cloth slightly dampened with water or a solution of water and a mild detergent 26 1260 Infinity MI User Manual Stator Face When Poorinjection volume reproducibility Leaking injection val
13. pendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the internet at http www chem agilent com 42 1260 Infinity MI User Manual Index Index A Agilent on internet 42 APG remote 12 B buffers 25 C capillary connections 13 cleaning the manual injector 26 D damaged packaging 8 delivery checklist 8 description 6 E exchanging injection seal 29 position sensing switch 32 stator face 27 F flow cell solvent information 18 flow connections 13 flushing the manual injector 25 information oninternet 42 INJECT 6 22 1260 Infinity MI User Manual injecting sample 6 injection seal 29 tefzel 19 vespel 19 installation 9 installing the manual injector 9 internet 42 42 L leak channel 15 leak drainage 15 leaks 13 LOAD 6 21 M make before break 6 needle type 20 needle port cleaner 25 needles 20 P position sensing switch 32 position sensor cable 12 precision 21 repair procedures injection seal 29 position sensing switch 32 stator face 27 Rheodyne 77251 6 S salt solutions 25 sample loops 6 sample volume 21 solvents acids 18 buffers 25 ethers 18 rinsing 25 salt solutions 25 steel corrosive 18 start cable 12 stator face 27 U unpacking damaged packaging 8 delivery checklist 8 Ww worldwide web 42 43 www agilent com In This Book This manual contains user informatio
14. product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1260 Infinity MI User Manual In This Guide In This Guide This manual covers the Agilent 1260 Infinity Manual Injector G1328C 1 Introduction Operation and mechanical hardware 2 Installing the Manual Injector Installation of the manual injector 3 Using the Manual Injector How to use the manual injector 4 Maintenance Instructions on simple routine repair procedures 5 Parts and Materials for Maintenance Detailed illustrations and lists for identification of parts and materials 6 Appendix Additional information 1260 Infinity MI User Manual Contents Contents Introduction 5 Introduction to the Manual Injector 6 Installing the Manual Injector 7 Unpacking the Manual Injector 8 Installing the Manual Injector 9 Flow Connections 13 Leak Drainage 15 Using the Manual Injector 17 Solvent Information 18 Choice of Injection Seal 19 Needles 20 Injecting Sample 21 Maintenance 23 Overview of Maintenance 24 Flushing the Manual Injector 25 Cleaning the Manual I
15. ring Notch Needle hole 30 1260 Infinity MI User Manual Maintenance 4 7 Insert the stator face onto the stator head 8 Install the stator head and stator face onto the valve Ensure the pin in the stator ring is aligned with the hole in the stator head 9 Secure the stator face and stator head in place with the stator screws Tighten each screw alternately 4 turn until the stator head is secure f M ES 1260 Infinity MI User Manual 31 4 Maintenance Position Sensing Switch When Nostart signal when switching to the inject position Tools required Hex key 9 64 inch supplied with valve Parts required p n Description 1 0490 1849 Position sensing switch 1 Loosen the three stator screws 2 Remove the stator head and stator face see Stator Face on page 27 32 1260 Infinity MI User Manual Maintenance 4 Position Sensing Switch 3 Remove the stator ring 4 Disconnect the sensor cable from the start cable Pull the sensing switch out of the stator ring 5 6 Install the stator ring Ensure the pin in the stator ring is aligned with the hole in the valve body ey 1260 Infinity MI User Manual 33 4 Maintenance Position Sensing Switch stator screws Tighten each screw alternately 74 turn until the stator head is secure 7 Insert the stator face onto the
16. stator head 8 Install the stator head and stator face onto the valve Ensure the pin in the stator ring is aligned with the hole in the stator head 9 Secure the stator face and stator head in place with the cr SSs 0 O e CAII 9 Im 34 1260 Infinity MI User Manual 1260 Infinity MI User Manual e a e ee 5 e Parts and Materials for Maintenance e Manual Injector 36 Injection Valve Assembly 38 Detailed illustrations and lists for identification of parts and materials E Agilent Technologies 35 5 Manual Injector 36 Parts and Materials for Maintenance Item p n 1 gt wo N 5063 6502 5001 3738 G1328 44111 5042 8553 5042 8576 5042 8901 5182 9619 G1328 87600 0100 1677 Description Manual injection valve Mounting pole Base plate Organizer plate Catch tube cap Name plate Valve syringe fixed needle 50 pL Connection capillary 0 17 mm id 500 mm Start cable Manual injector valve see Injection Valve Assembly on page 38 1260 Infinity MI User Manual Parts and Materials for Maintenance 5 Manual Injector Figure 12 Manual Injector 1260 Infinity MI User Manual 37 5 Injection Valve Assembly 38 Parts and Materials for Maintenance Manual injection valve p n 5063 6502 with starts cable complete assembly including operating instructions needle port cleaner vent tubes x2 and fittings 5 64 and 9 64 inch hex keys Includes ite
17. ve Tools required Parts required p n 1 0100 1859 Hex key 9 64 inch supplied with valve Description Stator face Maintenance 4 1 Loosen the three stator screws Remove the stator head and stator face 1260 Infinity MI User Manual 27 4 Maintenance 4 Install the stator head and stator face onto the valve Ensure the pin in the stator ring is aligned with the hole in the stator head 3 Insert the new stator face onto the stator head 5 Secure the stator face and stator head in place with the stator screws Tighten each screw alternately 74 turn until the stator head is secure f M ES 28 1260 Infinity MI User Manual Maintenance 4 Injection Valve Seal When Poorinjection volume reproducibility Leaking injection valve Tools required Hex key 9 64 inch supplied with valve Parts required p n Description 1 0100 0623 Rotor seal Vespel 0100 0620 Tefz el 1 Loosen the three stator screws 2 Remove the stator head and stator face see Stator Face on page 27 C ey IANA N N 97 Ase ay 1260 Infinity MI User Manual 29 4 Maintenance 3 Remove the stator ring 4 Remove the seal 5 Install the new seal Ensure the seal is positioned as 6 Install the stator ring Ensure the pin in the stator ring is shown aligned with the hole in the valve body Rotor pin Stator
18. written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the
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