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MCPC618 Multichannel Photon Counting System

1. Filter Criteria F1 F2 Block Data Transmission For This Event If Filter Criteria Not Met Figure 17 Discriminant Definition Pane With the product term definition shown above the data filter function will generate a match only if the average of channels 3 4 and 5 is greater than 60 counts and the average of channels 6 and 7 is less than 70 counts The records that meet this criterion will have their corresponding data filter match bit set in the log file However because the Block Data Transmission box is not checked all records will be logged regardless of the match condition xd Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Log Files The Control and Acquisition Interface Software produces binary log files during data collection that can be viewed using the GUI display or processed off line for more thorough data analysis The GUI display function is accessed using the Log File View on the front panel This acquisition mode allows the user to step through and view individual count records in the binary log file More advanced data processing functions such as sorting and pattern detection can be applied by operating directly on the binary log files or by using spre
2. Record Count Record Index 646 Trig Time Stamp 14 2972859 Display Source Signal Photon Acquisition Processing Threshold MGATE Setpoint Disabled 2 Display Only Background Subtract 5 elle CO External Trigger Type Internal v E 1000 Hz Display amp Log CO Internal Falin Gain Compensation High Voltage TOR n Data Filtering P taa l D Log File Viewer O 850 0 With lt nsec 4 o Log Pan 4 Status y sss ox Figure 2 Control and Acquisition Software Front Panel 1 Pull Down Menus 5 Status Indicators 2 Main Display Area 6 Counters 3 Status Bars 7 Display Type 4 Acquire Button 8 Control Section 13 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Included Components and Software The PhotoniQ model MCPC618 comes enclosed in a rugged EMI shielded laboratory instrument case and is shipped with the following standard components and software e PhotoniQ Control and Acquisition Interface Software CD ROM e DC power supply 45V 2A with power cord e USB 2 0 cable 14 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Specifications System Specifications Item Number of Channels Input Impedance Input Preamplif
3. 46 mole At me 46 Pange BIS sexi tnt deeg 46 Trigger Time Stamp EE 46 Count Ferd EN 46 Converting a Binary Log File to Text eee tnn tm tete dnne benene 47 6 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Configuration mc 48 User Configuration Table M L 48 Custom Configuration Table 53 Factory Configuration Table 53 DLL Function Prototypes Lois dno ponddncocndod bod oeque ER 56 Function sd 000100 nnns e rnn n Ennn 56 11121 ER 56 GIOS e e 56 GT AGE Rm 56 BEE EL gt 57 lead 58 BENEDICITE EN 58 Error Cluster Jeunes eege eege 58 Control Interface CoOIImarids uiid peur Redde va d dde Edad vipera adve dedi de EpL EE XE RR DA 59 Low Level USB Interface Description rrnnnnnnvnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnr 61 USB Device D lais inn prt pn n pan Ern rnt Enn pau a Enea Eun Fue Een Fen Enn Fou En Fn dun p 61 HID siia m 61 Report Format IDS 0x01 and OXTT uode drops dresden reet edn etd 62 Report Format den EE 63 ue Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System List of Figures Figure 1 ee ERT E H 12 Figure 2 Co
4. YPlechnologies AP Technologies Limited The Coach House Watery Lane Bath BA21RL www aptechnologies co uk T 44 0 1225 780 400 F 44 0 8701 266 449 E info aptechnologies co uk User Manual PhotoniQ Series MCPC618 8 Channel Photon Counting System B Vertilon Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Disclaimer Vertilon Corporation has made every attempt to ensure that the information in this document is accurate and complete Vertilon assumes no liability for errors or for any incidental consequential indirect or special damages including without limitation loss of use loss or alteration of data delays lost profits or savings arising from the use of this document or the product which it accompanies Vertilon reserves the right to change this product without prior notice No responsibility is assumed by Vertilon for any infringements of patents or other rights of third parties which may result from its use No license is granted by implication or otherwise under the patent and proprietary information rights of Vertilon Corporation Copyright Information 2012 Vertilon Corporation ALL RIGHTS RESERVED CE Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System 4 Vertilon Corporation 66 Tadmuck Road Westford MA 0188
5. Flag 6 Disabled Flag 7 Disabled Flag 8 Disabled Figure 16 Flag Definition Pane mom Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Discriminant Definition The data filter match function is programmed in the Discriminant Definition pane as a logical combination of the previously defined flags utilizing a sum of products format Each row in the table is a grouping of flags that are logically AND d together The rows are then logically OR d to produce the filter result The Filter Criteria line shows the resulting equation with representing a logical AND and representing a logical OR Each record can thus generate only a true or false condition The user should only use flags in the discriminant definition that have been defined and enabled in the Flag Definition pane Checking the Block Data Transmission box in the Discriminant Definition pane forces record data that generates a false response to the filter criteria to be blocked from being logged or displayed Ei Spectral Data Filtering Spectral Filtering Configuration coo Band Filtering Flag Definitions Product Term Definitions Flags F1 F2 F3 F4 F5 F6 F7 F8 l au D Terms
6. 1 Input errors are similarly reported in the Input Error column If range bit reporting was enabled during logging the individual channel data columns will contain the value MAX depending on whether the count exceeded the maximum value of the input accumulator An input error on a particular channel is identified by the value ERR in its respective column in the table The Filter Match column contains a 1 when the count data met the filter criteria or a 0 when it did not If filter processing is not enabled this column is filled with 0 Due to conversion speed limitations the log file converter should be used on files containing less than 20 000 records Larger files will take a noticeable time to process 47 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Configuration Tables The hardware and software configuration of the PhotoniQ is stored in three separate tables user custom and factory configuration tables The sections that follow summarize the contents of the three tables Some configuration parameters are not used in certain PhotoniQ products Additionally parameter limits may differ depending on PhotoniQ model number User Configuration Table The user table contains the configuration of the PhotoniQ set by the user through the user interface It is 1000 words long and is described in the
7. The PhotoniQ model MCPC618 is supplied with a 5V desktop power source Use with any power source other than the one supplied may result in damage to the product Operate Inputs within Specified Range To avoid electric shock fire hazard or damage to the product do not apply a voltage to any input outside of its specified operating range Electrostatic Discharge Sensitive Electrostatic discharges may result in damage to the MCPC618 or its accessories Follow typical ESD precautions Do Not Operate in Wet or Damp Conditions To avoid electric shock or damage to the product do not operate in wet or damp conditions Do Not Operate in Explosive Atmosphere To avoid injury or fire hazard do not operate in an explosive atmosphere 10 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Product Overview The PhotoniQ model MCPC618 is a complete off the shelf high speed eight channel photon counting system for PMTs silicon photomultipliers SiPM and APDs Implemented as a stand alone laboratory instrument with a PC interface the MCPC618 is used for preamplification discrimination counting and data acquisition DAQ of single photon events across eight independent counting channels Its unique front end design permits direct connection to most PMTs without the need for an external preamplifier Flexible intelligent triggering allows the unit to reliably acquire count d
8. positive delay only relative to when the threshold was crossed In the example below the count period shift is one Ts interval Pre Trigger In pre trigger mode an external positive edge TRIGGER trigger signal is used to acquire count data that 15T5 ty occurred prior to the trigger s arrival As shown below the programmable pre trigger delay tpta is used to set the start of the programmable count period Top at a time prior to the trigger edge The pre trigger uncertainty time tptu Sun shown as the dashed area in the figure is equal to sampling period of the system Ts While the start of the count period is uncertain by time Ts OUT the actual duration of the count period itself is quite accurate Both the pre trigger delay and the count period are constrained to be multiples of the system s sampling period The trigger output signal is a reference signal that can be used to setup the system Regardless of the pre trigger delay time the leading edge of the trigger out always occurs between 0 and Ts from the leading edge of the trigger input signal The period of the trigger out is precisely equal to the count period When the pre trigger delay is set to one positive Ts the start of the count period precedes the rising edge of the trigger output by one half of sample period Ts For other pre trigger delay times either positive or negative the actual count period is shifted accordingly 24 Vertilon
9. www vertilon com MCPC618 Multichannel Photon Counting System Typical DNA Sequencer Setup DNA sequencing applications require the use of four or more photomultiplier tubes to detect the fluorescence from DNA fragments labeled with fluorescent dyes each dye indicating the presence of a DNA fragment with one of the four DNA bases T A G C A typical setup using a PhotoniQ MCPC618 four photomultiplier tubes optics a laser and a microcapillary electrophoresis array containing the DNA fragments is shown below The PMTs are positioned with the optics to detect the fluorescence from the DNA fragments labeled with the individual dye markers Each PMT connects to a photon counting input on the MCPC618 multichannel photon counting system The system is triggered to coincide with the firing of the excitation laser and each resulting record generated by the MCPC618 consists of the photon counts for the four PMTs accumulated during the user programmed count period The count data from the unit is sent to a PC over a USB 2 0 connection for display logging or real time processing MICROCAPILLARY ARRAY o gt j sc PHOTONIQ MCPC618 A 1 A T 1 G C A 1 gt OQON gt 0 gt gt N 10 gt gt 0Q gt O 04 000 TRIGGER PERIOD TRIGGER Figure 3 Typical DNA Sequencer Setup 18 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Th
10. 1771 1772 1773 1774 1775 1776 177T 1780 1781 1784 Parameter Name DSPRevCode FPGARevCode ChOBckgndOffset Ch255BckgndOffset ChOElecOffset Ch255ElecOffset SiteSerNum BoardSerNum SIBSpareControl SpeedDyRange HVPopulatedO HVPopulated1 BiasVoltage DREVoltage0 RESERVED ResetLowThresh0 ResetLowThresh3 ResetHighThresh0 ResetHighThresh3 Type 32 LONG 32 LONG 16 SHORT 16 SHORT 32 LONG 32 LONG 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT Description DSP Revision Code FPGA Revision Code DSP calculated background for each channel DSP calculated electrical offsets for each channel Unused Board Serial Number Unused Speed Dynamic Range for each bank nibble based High voltage supply 1 populated High voltage supply 2 populated Bias Voltage Control DAC 1 Can be configured for an alternative front end configuration DAC4 Reserved for expansion Reset low threshold for bank 1 to bank 4 Reset high threshold for bank 1 to bank 4 53 Parameter Limits None 0 OxFFFFFFFF None 0 OxFFFFFFFF 0 OxFFFF 0 OxFFFF None 0 OxFFFFFFFF None 0 OxFFFFFFFF Unused For each nibble 4 bits 0 Standard 1 16 Bit 2 14 Bit 0 Unpopulated 1 Populated 0 Unpopulated 1 Populated 0 4095 3 0V full scale 0 4095 3 0V full scale 0 OxFFFF 0 Ox
11. Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Count Period and Count Period Delay The count period is the time duration over which the input counts are accumulated The count period delay is the parameter that sets the start of the count period relative to the rising edge of the trigger Only for pre triggering can this value be negative Both count period parameters are adjustable Boxcar Mode Boxcar mode utilizes the input trigger signal to set TRIGGER the two count period parameters The preset values are ignored As shown in the figure the trigger signal is used to define the period over which the counts are to be accumulated Aside from a small amount of Meg fixed positive delay times tpca1 and toca the boxcar COUNT 1 formed by the trigger signal is the count period toc E and any counts that occur when the boxcar is bes SE inactive are not accumulated and therefore effectively masked out Micro Gate microGate The micro gate microGate provides an additional EXCITATION I TRIGGER level of control over the photon count period Although this function is technically not part of the MICRO me E intelligent triggering module it is closely related and Op in fact can be used with any of the intelligent triggering modes The example at right shows the simplest case where the microGate is used with edge trigger mode The exci
12. Count Accumulation One of the most powerful features of the MCPC618 is the wide variety of ways the count process can be triggered and controlled The unit consists of an intelligent trigger module with the capability to trigger the input channels in the conventional external or internal post trigger modes As an added feature advanced on board signal processing techniques permit more sophisticated triggering modes such as pre trigger which captures counts that occur prior to the trigger signal and input trigger which captures counts based on a threshold criteria The descriptions below illustrate some of the advanced trigger and count capabilities of the MCPC618 FRONT END EDGE TIMING TRIGGER GENERATOR INTERNAL TRIGGER LEVEL TRIGGER TRIGGER P3 ACQUISITION TIMING BOXCAR PROCESSOR GENERATOR GATE INPUT TRIGGER n DSP TIMING RIGGER GENERATOR TRIGGER CONFIGURATION TIMING CONFIGURATION Figure 7 Intelligent Trigger Module om Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Edge Trigger Edge trigger is a simple trigger mode whereby an externally supplied positive signal edge to the EDGE intelligent trigger module starts the counting process m 000 0 o As shown in the figure at right the rising edge of the trigger initiates the start of the count period top At the end of the count period a single record of data is j cr
13. Period Delay Used with Edge Input and Pre trigger types this parameter sets the delay from the trigger source to the start of the count period Negative values are permitted if Pre trigger is selected as the trigger type This parameter is ignored when Boxcar mode is enabled Count Period Used with all trigger types this parameter sets the duration of the count period For Inout and Pre trigger the period minimum is equal to the sample period Ts of the MCPC618 When using Input or Pre trigger only integer multiples of the sample period can be used as the Count Period This parameter is ignored when Boxcar mode is enabled 90 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Trigger Sets the trigger parameters for the acquisition process Type Used to select the trigger type of Edge Internal Level Input or Pre trigger For Edge Level and Pre trigger types the user supplies the trigger signal positive edge level to the trigger input BNC connector on the MCPC618 For Internal trigger type the unit supplies the internal trigger and therefore no external input is required Input triggering does not require a trigger signal but does require setting a threshold level Rate Used in conjunction with Internal and Leveltrigger types This parameter sets the rate of the internally generated trigger signal Threshold Sets the count threshold level for Input tr
14. Program Updates My Recent C3 LabVIEW Data Documents 2 My Data Sources My DVDs My Labels uy Music tB My Pictures My Received Files amy Shapes PS my Videos O Symantec updaters C Vertilon File name d My Network Save as type Custom Pattern log MI Select Cur Dir Figure 14 Select File Dialog Box The Log File Converter can also process binary files in a batch mode to save time when multiple binary files are to be converted Instead of browsing for a source file when the Select File button is pressed the user selects an entire directory by pressing the Select Cur Dir current directory button as shown in the dialog box above This effectively selects all binary files i e all files ending in log in the source directory for conversion to text files The target Select File button opens up a similar dialog box where the user selects the destination directory for the text files with the Select Cur Dir button Pressing the Convert button converts all files with the log extension in the source directory and places the resulting text files into the destination directory The target file names are identical to the source names except the file extension is changed from log to txt Note that since the batch mode of the Log File Converter attempts to convert all files ending in log into text files care should be taken to ensure that all log files in the source directory are val
15. Reserved for expansion Trigger Indicator LED On Period Trigger Indicator LED Off Period Acquisition Indicator LED On Period Acquisition Indicator LED Off Period CPLD Revision Code Model Number String SDRAM Type Populated 54 Parameter Limits 0 OxFFFF 0 OxFFFF 0 4095 3 0V full scale Bit 0 Deserializer Enable Bit 1 Reset Threshold Enable Bit 2 Buffer Enable Bit 3 Differencer Raw or Subtract Bit 4 Offset Enable Bit 5 Gain Enable Bit 6 Range Adjust Enable Bit 7 Data Trigger Enable 0 Disabled Raw 1 Enabled Subtract 0 OxFFFF Should never exceed 64 channels per bank 256 total channels Nibble based 4 bits nibble per bank signal polarity select 0 Sign Magnitude 1 Magnitude 0 TV1 Disabled TV2 Disabled 1 TV1 Enabled TV2 Disabled 2 TV1 Disabled TV2 Enabled 3 TV1 Enabled TV2 Enabled Factory calculated values Floating point calculation results 100 are entered into table Same As Above None 0 OxFFFF None Only letters are A F 1 0x32 1 0x32 1 0x32 1 0x32 0 OxFF None ASCII Codes 0 None 1 32 MByte Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Index 1834 1836 1837 1838 1839 1840 1841 1842 1843 1844 1999 Parameter Name SDRAMEnabled ProgScalingO ProgScaling1 ProgScaling2 ProgScaling3 RESERVED Typ
16. any additional information should be entered using the Arguments input Any returned information is available in the Returned Arguments output Opcode Selects the control operation to be performed Arguments Input for any additional information required by the selected control operation len Length of Arguments array TimeoutMs Specifies the time to wait for a response from the PhotoniQ Value entered in milliseconds errorlnNoError Accepts a standard LabVIEW error cluster Control operation is not performed if an error is present NumRetArguments Indicates the number of returned arguments ReturnedArguments Output for any returned information from the control operation len2 Length of ReturnedArguments array errorOut Points to error information from the function in a standard LabVIEW error cluster 56 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Datalnterface void cdecl Datalnterface LVRefNum fileRefnum LVRefNum BoolRefnum LVRefNum DigNumRefnum LVRefNum TrigCountRefnum unsigned long NumEvents double TimeoutS double TimeToCollect LVBoolean HighSpeedMode TD1 errorinNoError LVBoolean MessagingEnabled long MessagingArray long len long NumEventsRead LVRefNum dupFileRefnum LVBoolean NumEventsReached LVBoolean TimeoutReached LVBoolean TimeToCollectReached unsigned short ImmediateEventData long len2 double El
17. event data sent from the PhotoniQ using report ID 0x22 will have the format specified in the following table Note that indices here are specified for shortword data Note that an HID class driver will remove the Report ID before retuming any data and indices should be adjusted accordingly Index 0 1 3 4 cO co N O Ci 10 11 Length 10 Length 11 Value Report ID MSByte must be 0x00 Fixed Start Codon ASCII string DAT Opcode 0x0099 Length Number of data words Number of Events in Report Words per Event Number of Remaining Available Reports Trigger Count L Trigger Count H Data Checksum Sum of all values including checksum equals zero Table 17 Report Format ID 0x22 63 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System EZ Vertilon Vertilon Corporation has made every attempt to ensure that the information in this document is accurate and complete Vertilon assumes no liability for errors or for any incidental consequential indirect or special damages including without limitation loss of use loss or alteration of data delays lost profits or savings arising from the use of this document or the product which it accompanies Vertilon reserves the right to change this product without prior notice No responsibility is assumed by Vertilon for any infringements of patent
18. non standard types can be found in the header files PhotoniQ h and extcode h Initialize void __cdecl Initialize long BufferSize TD1 errorlnNoError unsigned long Version TD1 errorOut Opens and initializes an interface to a PhotoniQ Sets the amount of buffering used in USB communications with the PhotoniQ and returns the USB firmware version number from the PhotoniQ BufferSize Sets the amount of buffering used in USB communications with the PhotoniQ Valid range is 8 200 Larger numbers use more buffering which helps keep the throughput of the interface maximized errorinNoError Accepts a standard LabVIEW error cluster Initialization is not performed if an error is present Version ndicates the USB firmware version number errorOut Points to error information from the function in a standard LabVIEW error cluster Close void cdecl Close TD1 errorinNoError TD1 errorOut Closes the interface to a previously initialized PhotoniQ errorinNoError Accepts a pointer to a standard LabVIEW error cluster errorOut Duplicate error in cluster output Controllnterface void __cdecl Controllnterface unsigned short Opcode unsigned short Arguments long len long TimeoutMs TD1 errorinNoError unsigned short NumRetArguments unsigned short ReturnedArguments long len2 TD1 errorOut Executes a control operation to a previously initialized PhotoniQ The Opcode input specifies the operation to be executed and
19. table below Index 0 N DO 7 P C no CO 29 30 33 34 37 38 41 Parameter Name SystemMode HVLimitO HVLimitl NumChannelsBO NumChannelsB1 NumChannelsB2 NumChannelsB3 HVEnabled HVSetpointo HVSetpoint1 UserConfiglD DCRD AOut 0 BandEnables BandOStartIndex BandOEndlndex Band Indices for Remaining Bands FlagEnables FlagOOperandO FlagOOperand3 Flag1OperandO Flag Operand3 Flag2OperandO Flag2Operand3 Type 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT Description Indicates current system mode acquire or standby mode Maximum allowed voltage on HV supply 1 Maximum allowed voltage on HV supply 2 Number of channels enabled bank 1 Number of channels enabled bank 2 Number of channels enabled bank 3 Number of channels enabled bank 4 Enables for high voltage supplies Current setpoint HV supply 1 DAC 6 Current setpoint HV supply 2 DAC 7 Unused Daughtercard analog out control DAC 8 Spectral filtering band enables Start index for spectral filtering band 1 End index for spectral filtering band 1 Start index for spectral filtering band 2 8 End index for spectral filtering band 2 8 Spectral filtering flag enables Spectral filtering operands for flag 1 configuration Spectral filtering operands for flag 2 c
20. the display window The trigger stamp is the running total of all triggers seen by the system since the start of the Acquire period Time stamps are taken in fixed resolution steps as determined in the Data Configuration pull down menu and are referenced to the start of the Acquire period The Trigger Time Stamp counter rolls over after the maximum value is reached To enable this feature the Trigger Time Stamp must be selected in the Data Configuration menu ET Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Display Selects the type of data plotted on the display The logged data and processing functions are unaffected by these selections Signal The count for the eight input channels is plotted on the real time display If Background Subtraction is enabled the raw input signal minus the background is displayed Background Only the pre calculated background signal is plotted on the real time display Select this display function when initially configuring the system to minimize the background optical signal or dark count This function is only available if Background Subtraction processing is enabled Channel The horizontal channels 1 through 8 for display are selected using this feature 34 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Pul
21. the total number of count records in a log file when in Log File View mode The Record Count and Trigger Count are the only two indicators active when in Log Only acquisition mode Note when the unit is in the Display Only or Display amp Log acquisition modes the Record Count will usually be much less than the Trigger Count because the overhead from the real time data display significantly slows the count record acquisition rate The Log Only acquisition mode on the other hand is a high speed data acquisition mode that is able to keep up with the trigger rate provided it is within the specified limits Under these conditions the Record Count will usually equal the Trigger Count after the acquisition period ends and all records will be transferred to the PC However even in this mode it is possible for the Record Count to be less than the Trigger Count This can occur if the maximum trigger rate specification is exceeded even momentarily or if the Acquire button is pressed while active triggers are input to the system To avoid the latter situation the Acquire button should be activated before any triggers are applied to the system Record Index Available only in Log File View mode this box allows the user to scroll through records or to enter a specific record number for viewing from the log file The maximum record index is equal to the record total Trigger Time Stamp Shows the trigger or time stamp for the record currently displayed in
22. words The record consists of a single word header followed by one additional word of count data for each channel enabled in the system Depending on the configuration there may be additional words following the count data The figure below shows a generic example of a count record for a system configured with reporting for Range Bits and Trigger Time Stamp TS1 TS2 enabled The MCPC618 produces a maximum of 12 data words per trigger 9 data words with range and trigger time stamp words off when all eight channels are enabled HEADER CH1 CH2 CH3 CH4 CH5 CH6 CH7 CH8 Figure 18 Count Record Format 45 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Header Word The contents of the count record header word are detailed in the table below Bit Function Description 15 13 Record Type 100 Normal Record 12 Out of Range 0 No Faults Detected in Record Fault At Least 1 Fault Detected in Record 11 Input Error 0 No Faults Detected in Record Fault At Least 1 Fault Detected in Record 10 6 Reserved Reserved for Future Use 5 Filter Match 0 Filter Condition Not Met for Record or Filtering Not Enabled t Filter Condition Met for Record 4 0 Filter Match Library Number of Filter Match Library Number Don t Care if No Filter Match currently unsupp
23. 00 0 05 100000us Range 400000 10000000 4000us 100000us Range 0 OxFFFF Range 0 OxFFFF Range 0 OxFFFF Range 0 OxFFFF Range 0 OxFFFF Range 0 OxFFFF Range 0 OxFFFFFFFF 0 Disabled 1 Enabled 0 17 bit Sign Magnitude 1 16 bit 2 s Comp w shift FS 2 16 bit Ze Comp no shift HS 0 OxFFFF 0 OxFFFF 0 Disabled One bit per channel Range 0 255 each bit position corresponds to 1 of 8 band enables Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Index 679 680 681 694 695 696 699 700 703 704 707 708 711 712 715 716 719 720 723 724 727 728 735 736 737 738 739 740 741 742 743 Parameter Name MBandoStartIndex MBandOEndindex MBand Indices for Remaining MBands MFlagEnables MFlagOOperando MFlagOOperand3 MFlag1 Operando MFlag1Operand3 MFlag2Operand0 MFlag2Operand3 MFlag3Operand0 MFlag3Operand3 MFlag4Operand0 MFlag4Operand3 MFlag5Operand0 MFlag5Operand3 MFlag6Operando MFlag6Operand3 MFlag7Operand0 MFlag7Operand3 MPTerm0 MPTerm7 MDataFilterEnable MDataFilterConfig MDataFilterAChannels MDataFilterBChannels MDataFilterA MDataFilterB Display Setting Bar32Channels Type 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16
24. 6 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Table of Contents List of Fig re err TE 8 List of Tables siisii indir ONU UU MN ae 9 General Safety Precautions uuicaaaes ege r6nao ka ble Fa nau asa eR EE naken 10 Product OVGIVICW 11 FEE etic T 11 elei 1119 NE Ed UE 12 MP 12 nr EEE 13 Included Components and Gofhware une 14 Pee 15 System Specifications cccccceccceceeeeeeeeeeeeeeeeeeeeeeeeeeeeqeaeeeeeeeeeeeeeeeceeeeeeeeeeeeseeceeeneeeeeeess 15 Trigger and Count Period Specifications AEN 16 Miscellaneous SpecifICallOns suus noc ttn dr tane petendi rede tede dicet dioi 17 Mechanical eene e EE 17 PC System Requirements MRT 17 Typical DNA e e au bnnc EX tain a ewe 18 Theory of Operation E A M 19 eredi gre ge 19 19 CNN t Tm 19 BN ee EG 9 EE EET m 19 Pipelined Parallel e E 19 Digital Signal Ge EE 21 Control and Acquisition Interface Software xrrrrnnnrvrrrnnnnnnnrrnnnnvrrrrnnnnnnnnrrnnnnnrrrnnnnnnenennn 22 Intelligent Triggering and Count Accumulation urarrrnnnnnrrrvnnnnnnnnnnnnnnvrrrnnnnnnnnrennnnnrrnnnnnne 22 FETE 23 Med OE iate ooo E ea ed Era etie ed Ep diee on Exe 23 TMG eH de ers ved See i eerste 23 NADIE Lie e PR 24 ial m Q 24 Count Period and Count Period Delai 25 Boxcar Mode ee TEEN 25 Micro Gate microGate ie scpe
25. A 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Opcode Function Name Description OxBB Erase System Code Erases current DSP or FPGA system code Available only when running the PROM PROM Bum Burn code Input Arguments 0x55 OxAA and OxFO for FPGA code OxOF for DSP code Return Arguments Error if necessary 0xCC Program System Programs one line of DSP or FPGA system code Available only when running the Code PROM Burn code PROM Burn Input Arguments 0x55 OxAA OxFO FPGA code or OxOF DSP code Line from an Intel Hex 32 formatted programming file Return Arguments Error if necessary Table 12 Control Interface Commands 60 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Low Level USB Interface Description A description of the low level interface to the PhotoniQ using the USB port is provided for programmers who wish to write their own set of DLLs or drivers The sections below summarize the details of the interface USB Device Defaults Value USB Compatibility Vendor ID Product ID Device ID Class Indexed String 1 Indexed String 2 Indexed String 3 Indexed String 4 HID Implementation Details USB 2 0 High speed 0x0925 0x0480 0x0000 Human Interface Device HID 1 1 Vertilon PhotoniQ High when connected to high speed host Full when
26. FFFF Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Index 1785 1788 1789 1792 1793 1794 1795 1798 1799 1802 1803 1804 1805 1806 1807 1808 1809 1810 1811 1812 1813 1814 1815 1816 1817 1832 1833 Parameter Name OORLowThresh0 OORLowThresh3 OORHighThresh0 OORHighThresh3 VBTest0 VBTest1 ChProcessingEnablesO ChProcessingEnables3 NumChPopulated0 NumChPopulated3 SignalPolarity TestVoltageEnable HVOParameterO HVOParameter1 HV1Parameter0 HV1Parameter1 AssemblyRevisionPCRev AssemblyRevisionLetter RESERVED X1 Y1 X2 Y2 CPLDRevCode ModelNumber SDRAMPopulated Type 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT Description Out of range low threshold for bank 1 to bank 4 Out of range high threshold for bank 1 to bank 4 Test voltages DAC2 and DAC3 Channel processing enables for bank 1 to bank 4 Number of channels populated for bank 1 to bank 4 Signal polarity Test voltage enables bank 1 to bank 4 High voltage supply 1 normalization parameters High voltage supply 2 normalization parameters PCB Revision Number Assembly Revision Letter
27. ImageAcqMode InputTrigThresh InputTrigChannel RangeErrorEnable CrossBankConfig ReportPackingMode GPOutputEnable GPOutputDelay Type 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 32 LONG 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 32 LONG Description Spectral filtering operands for flag 4 configuration Spectral filtering operands for flag 5 configuration Spectral filtering operands for flag 6 configuration Spectral filtering operands for flag 7 configuration Spectral filtering operands for flag 8 configuration Spectral filtering product terms Spectral filtering data filter blocks data output if there is no spectral filter match Enables for various signal processing options Enables Disables timestamp output SIB analog out control DAC 5 Timestamp interval configuration Enables Disable custom words output Number of custom words Unused Image Acquisition Mode Enable Input trigger threshold Input trigger current channel Enables Disables range and error output Current cross bank configuration Indicates high speed or real time acquisition Enables Disables general purpose output General purpose output delay 49 Parameter Limits Same as Above Same as Above Same as Above Same as Above Same as Above Range 0 255 each bit position cor
28. NULL TD1 errOut LVFALSE 0 NULL This initialization will create the equivalent of a No Error cluster for use with the DLL functions The individual functions will update the errOut cluster if an error is detected during the execution of that function 58 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Control Interface Commands The command op codes for the control interface Controllnterface are given in the table below Opcode 0x03 0x04 0x06 0x07 0x09 0x0B OxAA Function Name Update PhotoniQ Configuration Read PhotoniQ Configuration Read ADCs Calibrate Report Update System Mode Re boot for FW Update Description Updates the PhotoniQ configuration by writing parameters to the PhotoniQ User Configuration Table Input Arguments An unsigned 16 bit number followed by an array of unsigned 16 bit configuration table parameters A zero as the first argument indicates a write of the configuration table to RAM only while a one indicates a write to flash memory Return Arguments Error returned if necessary Reads the three sections of the PhotoniQ Configuration Table Input Arguments Single unsigned 16 bit number A zero indicates a read of the configuration table from RAM while a one indicates a read from flash memory Return Arguments Array of unsigned 16 bit configuration table parameters Performs a rea
29. SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT Description Start index for matrix filtering band 1 End index for matrix filtering band 1 Start index for matrix filtering band 2 8 End index for matrix filtering band 2 8 Matrix filtering flag enables Matrix filtering operands for flag 1 configuration Matrix filtering operands for flag 2 configuration Matrix filtering operands for flag 3 configuration Matrix filtering operands for flag 4 configuration Matrix filtering operands for flag 5 configuration Matrix filtering operands for flag 6 configuration Matrix filtering operands for flag 7 configuration Matrix filtering operands for flag 8 configuration Matrix filtering product terms Matrix filtering data filter blocks data output if there is no matrix filter match Matrix A B combine parameters Matrix A channel span in GUI Matrix B channel span in GUI Matrix A parameters in row column format Matrix B parameters in row column format Display mode for GUI graphs Channels for Bar 32 graph 51 Parameter Limits Range 0 255 1 channel per bit Range 0 255 1 channel per bit Range 0 255 1 channel per bit Range 0 255 each bit position corresponds to a flag enable Flag0Operand0 2 Range 0 32767 FlagOOperand1 3 Range 0 7 or 65535 1 channel per bit or LSB wgt 65535 Same as Above Same as Above S
30. Software supports three types of acquisition modes for real time display and or logging of count data from the MCPC618 hardware A fourth acquisition mode allows the user to view a logged file in the display area Display Only This mode is intended for use in setting up the user s system when the real time impact of modifications is needed such as during optical alignment detector bias selection or discriminator threshold adjust Most of the front panel functions are accessible Data is collected from the MCPC618 one record at a time and displayed in the display area in the GUI Additional triggers are ignored until the display is completely updated The processing overhead necessary to display the data reduces the maximum rate at which count records can be acquired Display amp Log Similar to the Display Only mode except that the user is able to log the viewed count records The display overhead reduces the maximum rate at which records can be logged without a loss of data Most of the front panel functions are disabled in this mode Log Only In this mode data from the MCPC618 is logged directly to a file With the exception of the Record and Trigger counters the display and front panel functions are disabled so that the maximum achievable logging rate can be attained Data acquisition is optimized for the collection of continuous triggers Triggers to the unit are not accepted if the system is busy processing a trigger that was previously a
31. URATION PROGRAM PROGRAM INTERNAL EXTERNAL VO Figure 6 DSP Functional Block Diagram 21 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Control and Acquisition Interface Software The MCPC618 is programmed and monitored by the Control and Acquisition Interface Software This software which is resident on the PC provides a convenient GUI to configure and monitor the operation of the unit Configuration data used to control various functions and variables within the MCPC618 such as trigger and acquisition modes count period processing functions etc is input through this interface For custom user applications the GUI is bypassed and control and acquisition is handled by the user s software that calls the DLL supplied with the unit As configuration data is modified the MCPC618 s local volatile RAM memory is updated with new configuration data The hardware operates based upon the configuration data stored in its local RAM memory If power is removed from the MCPC618 the configuration data must be reprogrammed through the GUI However a configuration can be saved within the non volatile flash memory of the unit At power up the hardware loads configuration data from its flash memory into its volatile RAM memory Alternatively the RAM memory can be configured from a file on the user s PC Intelligent Triggering and
32. adsheet based routines on text log files If text file format is desired a function included with the Control and Acquisition Interface Software is used to convert the binary log files to text log files Binary Log File Format Binary log files are used to minimize the time required to transfer the data from the MCPC618 to a hard disk on a PC To reduce processing overhead and storage requirements it is recommended that any off line data manipulations operate on this type of file The contents of the binary log files written by the Control and Acquisition Interface Software can be broken into three main sections the identification text header the configuration table and the data block The D Text Header defined in Table 5 below is a simple header that identifies the PhotoniQ model number date time 24 hour format and version information It is organized along 8 bit byte boundaries E 17 Offset Bytes Contents 0 Product ID Vertilon xoooox CR ILF 17 Date Time String 19 MM DD YY HH MM xx CRIILF 36 Software UI Version 28 LabVIEW UI Version xxxxxxx CR LF Table 5 Binary Log File ID Text Header Section The Config Table section shown in Table 6 contains configuration information relating to the MCPC618 hardware and firmware Unlike the D Text Header section the Config Table section is organized as 16 bit words instead of 8 bit bytes The configuration data is partitioned into three tables user custom and
33. affected when any of these items are selected See section on Log Files for the specifics on the log file sizes Data Configuration Configuration Settings Channels 8 Range Bits Trigger Time Stamp Time 100 nsec ze Figure 10 Data Configuration Dialog Box Channels Configures the number of input channels used by the system which in turn determines the size of the output data packets Range Bits Inserts out of range OOR and input error ERR data for each channel into the log file The range data is reported for each channel in each record Out of range occurs when the photon count during the count period exceeds the accumulator s maximum range An input error is reported when a DC condition is detected on the counting input Regardless of whether range bits option is selected the header for each record will contain data to indicate if at least one of the channels in the count record is out of range or has an input error Trigger Time Stamp Inserts a two word trigger or time stamp at the end of each record in the log file The selection choices are Trigger Time 100nsec Time 1 usec Time 10 usec Time 100 usec Time 1 msec and Off No trigger or time stamp is inserted into the log file if Off is selected The Trigger option inserts the absolute count of the number of triggers seen by the system for each record that is acquired The trigger stamp is reset to zero at the start of A
34. al Specifications snssnnenneeeeeeeeenrnrnneeseertnrnnnnnnererrrnnnnnnnnneeeennna 17 Table 5 Binary Log File ID Text Header Gechon 44 Table 6 Binary Log File Config Table Gecton 44 Table 7 Binary Log File Data Block Section cesseesssusssss 45 Table 8 Data Packet Header Word AEN 46 Table 9 User Configuration Table eteesteeriterbterbterkter terbterbterbtee rerbteebteebteeEreebteeEteebeee 52 Table 10 Custom Configuration Table 53 Table 11 Factory Configuration Table sssnssoneeeeeeeeeeeeeeeeeeeeenreeeerrrrerrreerereeerrereenne 55 Table 12 Control Interface Commande sse 60 Table 13 USB Device Details ccrte quias deed 61 Table 14 HID Report Descriptions uas cioe opa e oo emac e etae mu o enne caca a eege 61 Table 15 Report Format IDs 0x01 and GA 1 censurae rau akku ka eu xxu xni 62 Table 16 Report Error 006 eegenen dereen Leet eaa exin 62 Table 17 Report Format ID 0X22 egene ege EE deg 63 9 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System General Safety Precautions Warning High Voltages The PhotoniQ model MCPC618 interfaces to photomultiplier tubes avalanche photodiodes and silicon photomultipliers which require potentially harmful high voltages up to 2000 Volts during operation Extreme care should be taken Use Proper Power Source
35. ame as Above Same as Above Same as Above Same as Above Same as Above Range 0 255 each bit position corresponds to a flag 0 Disabled 1 Enabled Bit 0 Bar 32 Bit 1 Bar 64 Bit 2 Bar 128 Bit 3 Bar 256 Bit 4 Dual 4 x 4 Bits 8x8 Bit 6 Dual 8 x 8 Bit 7 16 x 16 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Index 744 745 746 747 748 749 750 751 752 753 754 755 756 757 758 759 760 Parameter Name Bar64Channels Bart28Channels Bar256Channels S8x8Channels D4x4ChannelsA D4x4ChannelsB D8x8ChannelsA D8x8ChannelsB 16x16Channels Bar32Attributes Bar64Attributes Bar128Attributes Bar256Attributes S8x8Attributes D4x4Attributes D8x8Attributes S16x16Attributes Type 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT Description Channels for Bar 64 graph Channels for Bar 128 graph Channels for Bar 256 graph Channels for single 8 x 8 graph Channels dual 4 x 4 graph A Channels dual 4 x 4 graph B Channels dual 8 x 8 graph A Channels dual 8 x 8 graph B Channels single 16 x16 graph Attributes for Bar 32 graph Attributes for Bar 64 graph Attributes for Bar 128 graph Attributes for Bar 256 graph Attributes for single 8 x 8 gra
36. apsedTimeS TD1 errorOut Collects data from a previously initialized PhotoniQ Options enable logging to a file programmable termination conditions and messaging data availability to another thread window Data is collected in Events where an Event consists of all data generated by the PhotoniQ in response to a single trigger event fileRefnum If a valid file refnum is entered in this control all data collected is logged to that file BoolRefnum Allows a calling LabVIEW panel to specify a Boolean control used to terminate data collection True Collect Data False End Collection and Return DigNumRefnum Allows a calling LabVIEW panel to specify a Digital Numeric control used to display the running total number of events collected TrigCountRefnum Allows a calling LabVIEW panel to specify a Digital Numeric control used to display the running total number of triggers from the trigger counter NumEvents Specifies the number of Events to collect The function will return after collecting the specified number of Events Set to zero to collect an indefinite number of Events TimeoutS Specifies the allowed time between Events If the specified time elapses between received TimeToCollectS HighSpeedMode errorinNoError MessagingEnabled MessagingArray len NumEventsRead dupFileRefnum NumEventsReached TimeoutReached TimeToCollectReached Events the function will return Set to zero to disable the timeout Value entered in
37. ata using one of several sophisticated triggering techniques The MCPC618 is fully configurable through the PC via its USB 2 0 port using an included graphical user interface Continuous high speed data transfers to the PC are also handled through this port Additionally a LabView generated DLL is provided for users who wish to write their own applications that interface directly to the unit Features e Includes eight independent counting channels with on board preamps and discriminators e Internal or external discriminator threshold control e Pulse pair resolution of less than 4 nsec e Maximum count rate greater than 250 MHz per channel for a total of 2 billion counts per second e Intelligent triggering supports standard edge internal level and boxcar modes e Advanced triggering capability supports pre triggering and input threshold crossing e Flexible control of counting period parameters such as delay width or external boxcar e Adjustable microGate provides additional level of count gating at sub nanosecond time resolution e Synchronization of microGate to external excitation source e Parallel high speed hardware processor unit performs real time data filtering and background subtraction e Programmable data filtering function for real time detection of predefined energy patterns or spectrums e Trigger stamping and time stamping with 100 nsec resolution e USB 2 0 interface supports high data transfer rates e Graphical User Interface GUI for
38. ax 978 692 7010 www vertilon com Control and Acquisition Interface Software Running Controlinterface exe will open the main window front panel of the Control and Acquisition Interface Software The front panel is generally used for display and control of the data acquisition process and reporting of the system s operational status Various pull down menus are used for setting the configuration of the MCPC618 and for performing diagnostic routines E PhotoniQ Control amp Acquisition Interface Vertilon MCPC618 EXVertilon Filter Out of Input Match Range Error Trigger Count Trigger End Count 14299 Trigger Rate 1001 Record Count 646 Trig Time Stamp 14 2972859 Display Source Signa Background Channel Channel Processing Threshold MGATE Trigger Setpoint 3 Disabled Sync Edge 3 Background Subtract 5 Ext External Rising z 5 DEE internal Internal Gain Compensation Data Filtering Acquire Log Path Status 4 Status OK Figure 9 Front Panel 27 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Control Area This area allows the user to define the acquisition triggering and count period parameters and to control system settings Acquisition The Control and Acquisition Interface
39. boxes which can be found under the Processing pull down menu Background Subtraction Enables subtraction of a pre calculated background signal from the total signal Gain Compensation Enables gain compensation of channel to channel non uniformities Sometimes used to correct for sensor quantum efficiency differences Data Filtering Enables the data filtering processor which can selectively accept or reject data records depending on a set of user defined conditions Threshold The threshold parameters for the discriminators are set in this area Setpoint A value of 0 to 100 of the threshold adjustment range This value is usually set experimentally since it depends on the PMT or SiPM gain and the size and shape of the input pulse External Enables the external threshold adjustment input and bypasses the internal setting High Voltage The high voltage functions are available only if the high voltage bias supply option is installed and activated in the High Voltage Supply dialog box found under the System pull down menu On Enables high voltage bias supply This function is available only if high voltage bias supply is enabled under the High Voltage Supply dialog box HV Setpoint Sets the output voltage of high voltage bias supply Cannot exceed upper limit set under the High Voltage Supply dialog box 29 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon co
40. ccepted To handle high peak trigger rates count data is stored in an on board buffer where it is then logged at a slower speed to the PC The maximum sustained data acquisition rate will vary depending upon the user s computer system Log File View Allows the user to select a previously logged file for viewing in the display area Records are stepped through using the record index box Acquire Select File Button Toggles between Acquire and Standby for display and logging acquisition modes Once a configuration has been set the user starts acquiring data by toggling this switch to Acquire When the Log File View acquisition mode is selected this button allows the user to select the log file for viewing Pushing the button opens a dialog box through which a data file can be selected for manual playback Log Path Indicates the location of the data file that has been selected for logging or viewing Status Line Status information and error messages regarding the unit s operation are displayed in this box The LED to its left side is green under normal operating conditions and turns red when there is an error condition 28 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Processing Allows the user to select which processing functions if any are applied to the data The parameters for the individual processing functions are entered into their respective dialog
41. cicacctasssacecectnncapastnagiencececsnacinndiansien si esseecicadtsecesapiancbentes 25 Hardware Interac 26 5 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Control and Acquisition Interface Software eee 27 fup Plc ee ee 28 PR MNS 6 t Pc 28 geed r 29 ln e el E 29 High Voltage eisni iaman e a Ea aa aae eae 29 Bee 30 Eg mr 30 MN 31 Real Time Display Area m 32 Eee Re 32 Kee DIETE nis naa aa aa aa aa Ne 32 Filter Match NR OT TTD 32 Q t of FANG ERR enean oaeiae eaa e aE aaea 32 Input E 32 die en 32 Trigger End Count e 33 Hie gai ooo omm 33 Gate M 33 FOG ONS 9 Me MEE 33 Record IndeX eC 33 He 8 E e MR cT 33 Boc ee 34 aV Down MENUS E inaa naani aiiai 35 IS UE 35 VIS IRE US 36 NN 38 E nt 39 Data Feld E 41 isis Ree 41 FR STON os cataracts T 42 Discriminant Definition T 43 Kon Fee mm 44 Binary tog File dE 44 Count Record DESCHOMOM EE 45 Count Record Format Leserne 45 Header 00 EE E EE EE
42. connected to full speed host 06032801 Table 13 USB Device Details The PhotoniQ implements the reports listed below for communication Report IDs 0x01 and 0x11 Feature Input and Output are used to send commands to the PhotoniQ and receive responses Report ID 0x22 Input only is used to transfer event data from the PhotoniQ to the host The opcodes that can be used with each report type are also listed Report ID 0x01 0x11 0x11 0x22 Type Feature Output Input Input Length Opcodes Bytes Hex 63 00AA 63 0003 0004 0006 0007 0009 000B 00BB 00CC 63 0003 0004 0006 0007 0009 000B 00BB 00CC 4095 0099 Table 14 HID Report Descriptions 61 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Report Format IDs 0x01 and 0x11 The commands sent to the PhotoniQ using report IDs 0x01 and 0x11 must have the format specified in the following table Note that indices here are specified for shortword data Index Value 0 Report ID MSByte must be 0x00 13 Fixed Start Codon ASCII string CMD 4 Opcode 5 Length Number of data words 6 Length 5 Data Length 6 Checksum Sum of all values including checksum equals zero Table 15 Report Format IDs 0x01 and 0x11 Responses to commands are returned using the same report ID Responses have a min
43. cquire mode Ideally the trigger stamp will increment by exactly one for each record An increment of greater than one indicates that one or more triggers were missed This usually indicates that the trigger rate exceeded the maximum trigger rate for the system 36 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com The five Time options are used to insert a time stamp with a programmable resolution from 100 nsec to 1 msec Like the trigger stamp the time stamp is reset to zero at the start of Acquire mode To obtain absolute time an absolute time stamp taken when the MCPC618 first enters Acquire mode and inserted into the header at the top of each log file can be added to the relative time stamps appended to each record The time stamp can function as a good diagnostic tool if trigger frequency needs to be measured High Voltage Supply Opens the dialog box shown below where the optional high voltage bias supply is configured i High Voltage Supplies Enables amp Limits Limit V EnableVilv EE Figure 11 High Voltage Supply Dialog Box Enable HV1 Allows optional high voltage bias supply 1 to be controlled from the front panel If this box is unchecked the supply is turned off and the front panel controls are disabled HV1 Limit Sets the voltage limit for high voltage bias supply 1 so that the user cannot select a set point above this level
44. d of the ADCs on the PhotoniQ Input Arguments None Return Arguments Results of eight ADC reads in an array of unsigned 16 bit values in the following order HV1 monitor HV2 monitor SIB HV Monitor 3 3VA 5V UF DCRD AIN1 DCRD AINO ADC Spare To convert codes to volts Codes 4096 scale factor Scale factor 3 for assembly rev 0 and rev 1 5 for assembly rev 2 Performs a system calibration Calculates either an offset or background calculation Offset calculation not recommended for users Input Arguments Three unsigned 16 bit arguments 0x55 0xAA and 1 to indicate offset calculation desired 2 to indicate background calculation Return Arguments Error if necessary Increments the number of reports that the PC can accept Input Arguments 0x55 OxAA and the increment to the number of reports allowed Return Arguments None this opcode does not generate a response Changes the system mode from acquire to standby or standby to acquire Input Arguments 0x55 OxAA and the new system mode 0 standby 1 acquire Return Arguments Error if necessary Reboots the DSP and determines if system should enter the main code or PROM Burn code Used for a system firmware update and available when running the main code or the PROM Burn code Input Arguments 0x55 OxAA and 1 to enter PROM Burn code 0 to enter Main program code Return Arguments Error if necessary EG Vertilon Corporation 66 Tadmuck Road Westford M
45. e 16 SHORT 32 SINGLE 32 SINGLE 32 SINGLE 32 SINGLE Table 11 Description SDRAM Type Enabled Bank 1 floating point programmable bit scale factor units of Coulombs Bank 2 floating point programmable bit scale factor units of Coulombs Bank 3 floating point programmable bit scale factor units of Coulombs Bank 4 floating point programmable bit scale factor units of Coulombs Reserved for expansion Factory Configuration Table 55 Parameter Limits 2 64 MByte 0 None 1 32 MByte 2 64 MByte None None None None Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System DLL Function Prototypes To accommodate custom application development the low level control and communication functions for the PhotoniQ have been provided in both a dynamic link library PhotoniQ dll and an import library PhotoniQ lib The provided header file PhotoniQ h contains the required function prototypes typedefs and other definitions contained in extcode h which is included in PhotoniQ h and is also provided Function Prototypes The DLL prototype functions use the standard C calling convention and require the run time engine for LabVIEW version 9 0 The five functions provided in the file PhotoniQ dll are described below The Windows XP API is leveraged by each of these functions Typedefs for
46. eated that contains the total counts for each input ine channel configured The count interval parameters of il Lo delay to start tia and count period tcp are programmable over a large range of values with very fine resolution WE gt ts Internal Trigger Continuous data acquisition is possible by operating u the unit in internal trigger mode Here a INTERNAL programmable internal free running clock tax a replaces the external trigger signal Count data is j accumulated during the count period which occurs synchronously with each edge of the clock signal One data record containing the counts for all BS configured channels is generated per clock This PERIOD mode is particularly useful when large count periods are needed for collection and analysis but no trigger signal is available Level Trigger This trigger mode is similar to internal triggering Eo EN ams AEN except that an externally provided positive level p is sensitive trigger gate controls the counting The INTERNAL I i actual trigger signal is internally generated but synchronized and gated by the external trigger gate logic high enables the acquisition of data by allowing the internal trigger to generate the pre programmed count period top A logic low on the to trigger gate blocks the internal trigger from count E Tem generating the count period so that no further count rec
47. el 8 INTELLIGENT TRIGGER ACQUISITION Figure 4 Functional Block Diagram INPUT CHANNELS 18 PIPELINED SIGNAL PROCESSING CHANNELS 1 8 HEVANE gt gt POST REAL TIME CHANNEL acid PRE TRIGGER UNIFORMITY DATA BUFFER POST FILTER CORRECTION PROCESSOR INTELLIGENT TRIGGERING Figure 5 Pipelined Parallel Processor 20 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Digital Signal Processor The 16 bit fixed point digital signal processor performs the high level data manipulation and system control in the MCPC618 Data received from the P3 on the PPI is routed through the DSP and buffered using the on board SDRAM This architecture allows the unit to capture very large frames of data with little or no loss of data Once the data is stored itis packetized by the USB packet generator and sent out to the PC through the USB 2 0 port Extra computational power is reserved in the DSP so that user defined algorithms can be executed on the data prior to transmission This has the benefit that routines that were previously performed off line by the PC can instead be handled in real time The net effect is that the downstream data load to the PC is reduced so that throughput can be increased by orders of magnitude REAL TIME USB USB FRAME PACKET CONTROL PROCESSOR GENERATOR COMMAND PROCESSOR P3 P3 DSP IN CIRCUIT IN CIRCUIT CONFIG
48. eory of Operation The functional block diagram for the PhotoniQ MCPC618 shown in Figure 4 is made up of eight counting channels each consisting of an ultra high bandwidth preamplifier precision discriminator and high speed count accumulator The counting channels are configured and triggered together but operate independently The intelligent trigger acquisition module loads the triggering and acquisition parameters for the eight channels so that any one of multiple triggering modes can be used to control the count period and initiate the data acquisition process At the end of the count period eight parallel digital data channels are output to the Pipelined Parallel Processor P3 where it performs real time data filtering buffering and channel uniformity correction The resulting data is sent to the DSP where it is packetized and sent to the USB output port Additional reserved DSP processing power can be used to implement user defined filter trigger and data discrimination functions Counting Channels The front end preamp in each counting channel provides low noise high gain for the narrow pulses typically produced by photon counting photomultiplier tubes Its robust design and stable operation allows the MCPC618 s inputs to connect directly to a PMT s anode using any reasonable length of 50 ohm cable This avoids having to locate preamps close to the PMTs and having to deal with the associated power and control issues The gain of the pream
49. escence spectral pattern and reject all others Parameters for the filter are entered in three tabbed panes in the dialog box under the Spectral Filtering option in the Processing menu The data filtering processor operates on spectral bands defined by the user in the Band Definition pane according to a Boolean expression defined in the Flag Definition and Discriminant Definition panes Band Definition The Band Definition pane allows the user to create a set of up to eight frequency or position bands that are used to compare spectral or location regions respectively A band is defined as a continuous sequence of channels For example in the figure below Band 1 is defined as channels 3 through 5 and Band 2 as channels 6 through 7 Bands 3 through 8 are not defined It is not necessary to define all bands However care should be taken to not include unused channels in a band definition or unused bands in the Flag Definition described on the next page E Spectral Data Filtering Spectral Filtering Configuration Band Filtering Flag Definitions Product Term Definitions Enabled Bands Channel Range v 1 1 32 64 96 iv s t5 Ceo EREECHEN een 1 32 64 96 128 64 96 64 28 TRIP PCI PB PERPE 64 96 Figure 15 Band Definition Pane 41 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multic
50. factory The usertable contains the configuration of the unit set by the user through the user interface Any custom configuration data is stored in the custom table Factory programmed read only configuration data is found in the factory table Offset Words Description Length Words Contents 32 Config Table Revision 1 1st 8 bits Major Rev 2 d 8 bits Minor Rev 33 User Config Table 1000 User Configuration Binary Data 1033 Custom Config Table 250 Custom Configuration Binary Data 1283 Factory Config Table 750 Factory Configuration Binary Data Table 6 Binary Log File Config Table Section 44 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com The Data Block section defined in Table 7 below is made up of records that contain the count data for each channel One record is created for each trigger that is acquired while logging The length L of the record is dependent on the configuration settings selected in the user interface Count record data is partitioned along 16 bit word boundaries Offset Words Description Length Words Contents 2033 Record 1 L First Count Record 2033 L Record 2 L Second Count Record 2033 n 1 L Record n L nth Count Record Table 7 Binary Log File Data Block Section Count Record Description Count Record Format Each trigger processed by the system generates a record of length L where L is in 16 bit
51. from the front panel 237 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Processing The MCPC618 s processing functions are configured through this pull down menu Background Subtraction The MCPC618 includes a processing function that continuously subtracts a pre calculated background level from the raw signal from each of the input channels This function is useful when the raw input signal is dominated by a stable DC background level or dark count By enabling the Background Subtraction processing the DC background signal is removed from each channel for each record so that only the actual desired signal can be displayed or logged Pressing the Apply button performs the background level computation on each channel The computed values are then used for the Background Subtraction processing if enabled Calculation of the background level should be initiated anytime the user changes the system parameters Note that Background Subtraction does not increase the dynamic range of the System nor does it remove the shot noise associated with the background Its main use is to improve the display of the data and simplify the post processing of the logged data It is also useful for optical system setup diagnostics Gain Compensation Gain compensation processing allows the user to normalize the outputs from the individual channels Th
52. from the function in a standard LabVIEW error cluster ErrorHandler void cdecl ErrorHandler TD1 errorinNoError LVBoolean OutputErrorResult char OutputErrorString long len TD1 errorOut Converts a LabVIEW Error Cluster generated by a PhotoniQ function and returns a Boolean Error Result and an Error String appropriate for display in a user interface errorinNoError Accepts a standard LabVIEW error cluster OutputErrorResult Trueif an error was present False if no error OutputErrorString Contains a description of the error present blank if no error len Length of the OutputErrorString array errorOut Duplicate error in cluster output LVDLLStatus MgErr LVDLLStatus CStr errStr int32 errStrLen void module All Windows DLLs built from LabVIEW in addition to the functions you export contain this exported function The calling program uses this function to verify that the LabVIEW DLL loaded correctly If an error occurs while loading the DLL the function returns the error errStr Pass a string buffer to this parameter to receive additional information about the error errStrLen Setto the number of bytes in the string buffer passed as errStr module to retrieve the handle to the LabVIEW Run Time Engine being used by the DLL Typically this parameter can be set as NULL Error Cluster Initialization The error clusters should be initialized by the user application as shown below TD1 errin LVFALSE 0
53. h the DSP and over the USB port Each trigger results in a count record being generated that contains the individual counts for each channel over the count period Additional data such as time stamping is also included in the data record Pipelined Parallel Processor The P3 Pipelined Parallel Processor shown on the next page is a dedicated high speed hardware processing unit that executes 8 parallel channels of computations on the 8 data streams from the front end counting channels Each channel processor performs real time data filtering buffering and channel uniformity correction The outputs from the 8 channel processors are sent to the frame post processor where additional frame formatted data manipulation is performed The frame post processor output is sent to the Parallel Peripheral Interface PPI where it is formatted and transferred to the DSP for further processing 19 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System microGATE CHANNEL 1 INPUT Channel 1 PROCESSOR EXPANSION INTERFACE CHANNEL 2 INPUT CHANNEL 3 INPUT CHANNEL 4 INPUT 8 CHANNEL PIPELINED PARALLEL PROCESSOR CHANNEL 5 INPUT Channel 5 CHANNEL 6 INPUT Channel 6 CHANNEL 7 INPUT 16 BIT DIGITAL SIGNAL PROCESSOR CG 5 CG p CHANNEL 8 INPUT Chann
54. hannel Photon Counting System Flag Definition Up to eight flags can be defined by the user in the Flag Definition pane The result of a flag computation on the spectral or position data is either true or false All eight flags have the same structure in which the operand on the left is tested for being greater than the operand on the right Within each operand the user selects either a constant corresponding to a number of counts or a multiplier for the average of one of the bands defined in the Band Definition pane This allows the data filter processor to compare a band to a constant or compare two independently scaled bands to each other Referring to the example below two flags Flag 1 and Flag 2 are defined in the Flag Definition pane Flag 1 is true if one times the average of Band 1 is greater than 60 counts and Flag 2 is true if one times the average of Band 2 is less than 70 counts The data discriminator operates on these two flags with a user defined function to determine if a filter match occurred Note the user should only use bands in the flag definitions that have been enabled and defined in the Band Definition pane EI Data Filtering Data Filtering Configuration Band Definitions Flag Definitions Product Term Definitions Enabled Flags wl Flag1 1 X Avg Band gt 60 k Counts xd v Flag2 Tro Counts SE n k Avg Bana Flag 3 Disabled Flag 4 Disabled Flag 5 Disabled
55. id binary log files If the converter encounters an invalid binary file the conversion process will abort and no files valid or invalid will be converted 40 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Data Filtering When the Data Filtering processing function is enabled each record is compared to a predefined filter criteria If the result is true records in the log file are tagged so that those that meet the filter criteria can be identified when subsequently displayed or analyzed To minimize the data processing load to the host processor a Block Data Transmission configuration switch is available to block records that do not meet the filter criteria from being logged or displayed When this switch is set only data that generates a true response to the filter criteria is transmitted Note since data filtering is a real time embedded DSP functions in the PhotoniQ a reduction in the maximum data acquisition rate can be expected when this function is enabled Spectral filtering is most useful in applications where the acquired data represents wavelength or frequency information It is also possible to use it in one dimensional linear positional applications Typically the spectral filter is configured to accept or reject records that meet a predefined criteria or discriminant For instance the filter can be setup to acquire records that match a particular fluor
56. ier Gain Input Preamplifier Bandwidth Pulse Pair Resolution PPR Minimum Detectable Pulse Amplitude Maximum Count Rate per Channel Count Period Range Maximum Count per Count Period Maximum Trigger Rate Sustained Trigger Rate 8 Channels Enabled Power Consumption MCPC618 Specifications 8 50 ohm 20 dB 400 MHz 4 nsec max 8mV 250 MHz 100 nsec to 1 sec 16 383 400 KHz 250 KHz 5 Watts typ Table 1 System Specifications Typical specifications at room temperature For15 mV 4 nsec FWHM pulse Count period of 50 nsec 415 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Trigger and Count Period Specifications Fo N Description Trigger to Count Period Delay Trigger to Count Period Jitter Pre Trigger Delay Pre Trigger Uncertainty Boxcar Count Period Start Delay Boxcar Count Period Start Jitter Boxcar Count Period End Delay Boxcar Width Resolution Count Period Count Period Error Internal Trigger Rate Trigger Threshold Range Sample Period microGate Delay Adj Range microGate Delay Adj Error microGate Delay Adj Resolution microGate Width Adj Range microGate Width Adj Error microGate Width Adj Resolution Sym lia tid tota totu tocat tbca2 thew top tep 1 tolk Ts tmgd tmgd tmgd tmgpw tmgpw tmgpw Table 2 Typical specificatio
57. iggering This level should not be confused with the threshold setting for the discriminators Channel Sets the channel number used for Input triggering sns Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Real Time Display Area The display area is used to give a graphical view of the data collected while in the Display Only and Display amp Log acquire modes For these modes the displayed data is obtained directly from the MCPC618 in real time Data is also shown in the display area when viewing a previously logged file in Log File View mode The display area and its associated control functions are disabled when Log Only is selected as the acquisition mode Graphical Display Displays the real time signal in total counts accumulated during the count period for each of the input channels Count data is also shown on the display when viewing a previously logged file in Log File View mode Display Limit Adjust Clicking the upper or lower vertical scale value allows the display limits to be adjusted Filter Match This function is active when the data filter processing is enabled It indicates when the displayed count record matches the filter criteria Out of Range Indicates when one or more channels in a displayed record are out of range Input Error Indicates when an input error has been detected on one or more chan
58. imum Length value of 1 so that each response can return an error indicator in the first data location 1 No Error 0 Error If an error is present another data word is added to the report in the second data location indicating the specific error A list of error codes is provided below Code Name Description 0x01 Erase Failed DSP or FPGA erase operation failed 0x02 Program Failed DSP or FPGA program operation failed 0x77 Configuration ID mismatch Factory configuration ID does not match user value 0x88 Communication Timeout A control transfer timeout occurred resulting in an incomplete packet OxAA Invalid Argument Argument is out of allowed range Returns an additional data value containing the index of the offending argument OxAB EEPROM Error USB erase or program operation failed OxAC EEPROM Bus Busy USB erase or program operation failed OxBB Invalid Number of Arguments System received an unexpected number of arguments for a given command 0xCC Invalid Command System received an unknown command opcode OxDD Invalid Length Receive data length does not match expected total length OxEE Invalid Start Codon System received an invalid start sequence CMD OxFF Invalid Checksum System received an invalid checksum from the host Table 16 Report Error Codes 62 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Report Format ID 0x22 The
59. is is helpful when compensating for channel to channel responsivity or quantum efficiency differences in PMTs and silicon photomultipliers The gain compensation dialog box shown in Figure 12 lets the user adjust each channel by a positive or negative percentage For example a positive 2 adjustment into a specific channel will effectively multiply the raw count data for that channel by 1 02 A negative 2 adjustment would multiply the raw count data by 0 98 The compensation coefficient range is 100 to 100 The coefficients default to 0 when gain compensation is disabled Pi Gain Compensation Gain Compensation Coefficients Chi Chi Ch3 Ch4 ChS Ch6 Chi Ch8 DEN mmm Figure 12 Gain Compensation Dialog Box Data Filtering Data Filtering is used to selectively display log or tag records that meet a specific user defined matching criteria It is described in more detail in the Data Filtering section 38 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Utilities Generate Diagnostic Report Automatically runs diagnostic routines and generates a diagnostic report using the current system configuration A trigger must be supplied either internal or external before this routine is run Log File Converter This utility converts the binary files log created during logging into tab delimited text files txt The readable text files can be used as i
60. l Down Menus The pull down menus are available at the top of the graphical user interface window File File operations generally consist of storing and retrieving configurations between the PC and the MCPC618 s volatile and non volatile flash memory Configuration information stored in volatile memory will be lost when power to the unit is removed The default configuration will be loaded on power up Configuration information stored in flash memory will be retained even when power to the MCPC618 is removed New Loads the MCPC618 with the default configuration Open Loads the MCPC618 with a stored configuration from a file on the PC Save Saves the current configuration of the MCPC618 to a file on the PC Save As Saves the current configuration of the MCPC618 to a new file on the PC Read from Flash Loads the MCPC618 with the configuration stored in the unit s flash memory Write to Flash Writes the current configuration of the MCPC618 to its flash memory Print Window Prints the current window Exit Closes the executable 55 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System System The MCPC618 s basic operation is configured through this pull down menu Data Configuration Opens the dialog box shown below where the unit s basic system parameters are configured The system speed and log file size are
61. m MCPC618 Multichannel Photon Counting System MGATE Controls the operation of the microGate function The microGate is synchronously locked to the user supplied microGate input on the front panel It is used to selectively enable or disable counting while the microGate is active Disabled External Internal Enables or disables the microGate function When set to External the actual microGate input on the MCPC618 front panel is used to control the gating the delay and width adjustments in the GUI are ignored For the Internal setting the delay and width of the microGate are set by the user in the GUI Sync Edge Synchronizes the microGate to either the rising or falling edge of the microGate input Delay Sets the delay from the rising or falling edge of the microGate input to the start of the microGate Width Sets the period of the microGate Gate Polarity Sets the polarity of the microGate so that counting can be either enabled or inhibited while the microGate is active Count Sets the count period parameters for the acquisition process Boxcar Available only with Edge trigger type Boxcar mode uses the externally supplied trigger signal to effectively set the count period delay and count period duration The preset count period parameters are ignored The count period starts immediately after the rising edge of the user supplied boxcar trigger signal The count period time equals the width of the boxcar signal Count
62. menu driven data acquisition and configuration e LabVIEW generated DLL for interface to user custom applications SE Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Applications Fluorescence Spectroscopy Fluorescence Lifetime Measurement Chemiluminescence Detection Bioluminescence Detection Photon Correlation Spectroscopy Bioaerosol Detection and Discrimination DNA Sequencing LIDAR Particle Sizing Optical Tomography of Biological Tissue ow Light Level Detection Flow Cytometry Single Molecule Detection Neutrino Detection Spatial Radiation Detection Confocal Microscopy Particle Physics Hardware The photo below shows the PhotoniQ model MCPC618 EH aamen ry ei e SI SE Figure 1 Model MCPC618 m42 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Software The screen shot below shows the main window of the Graphical User Interface GUI software included with the MCPC618 All control status and acquisition functions are executed through this interface PhotoniQ Control amp Acquisition Interface Vertilon MCPC618 Channel Cex E Vertilon Filter Out of Input Match Range m z Som Trigger Count Trigger End Count 14299 0 Trigger Rate Gate Time 1001 1 sec v
63. n words of each count record is given by the equation L 14 NC R 2 TS The settings include the Number of Channels NC and the reporting enables for the Range Bits R and Trigger Time Stamp TS The reporting enables are set in the Data Configuration menu and can be either 1 or a 0 46 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Converting a Binary Log File to Text Text log files should be used if a user wishes to import logged count data into a spreadsheet for further processing A built in routine is included in the GUI for the purpose of converting a binary log file log extension into a text file txt extension The output of this conversion is a file containing a time and date stamp header and the logged count records organized by row where each row represents the input count totals from each successive trigger The count records are stored as tab delimited numbers where the columns represent from left to right Packet Number Packet Type PT Out of Range OR Input Error IE Filter Match FM and the count totals for channels 1 through N Only configured channels appear in the log file unused channels are left out If enabled the Trigger Time Stamp TS is stored in the last column A 4 is always present in the Record Type column An out of range condition on any of the N data channels is identified in the Out of Range column by a
64. nels in a displayed record Counting input overloads are generally caused when a sizeable pulse is detected on the input to the counting channel s preamplifier Typically this is the result of a large amount of light incident on the detector such that individual photons overlap and therefore can no longer be separately detected A sustained DC light condition can also cause an input error Trigger Count This indicator keeps count of the absolute number of triggers seen by the system since the beginning of the Acquire period The counter is reset at the start of the Acquire period and effectively counts all triggers regardless of whether a trigger was accepted or rejected until the Acquire period ends If the Trigger Count equals the Record Count after the acquired data has been transferred to the PC then no triggers were missed Note that if the record rate is exceptionally high the displayed Trigger Count will slightly lag the actual trigger count measured by the system It is also important to note that unlike Log Only mode where the displayed Trigger Count will be equal to the Trigger End Count at the end of the acquisition period this will usually not be the case when using the Display and Display amp Log modes Although the system in these modes will accurately count the triggers and stop when the Trigger End Count is reached the final displayed Trigger Count will only indicate the number of triggers counted when the last count record was acqui
65. ns at room temperature A fixed delay of approximately 25 nsec is in addition to the delay setting Relative to system sample period Ts A negative value for the delay corresponds to a pre trigger condition A fixed delay of approximately 20 nsec is in addition to the delay setting Trigger Mode Edge Edge Pre trigger Pre trigger Boxcar Boxcar Boxcar Boxcar Edge Internal Level Boxcar Input Pre trigger All Internal Level Input All All All All All All Minimum 0 nsec 10Ts 25 nsec 25 nsec 100 nsec 100 nsec 100 nsec 100 nsec Ts Ts 10 Hz 10 Hz 1 count 2 25 USEC 5 nsec 2 nsec 4 nsec 2 nsec Trigger and Count Period Specifications 16 Maximum 1 msec 5 nsec 1000Ts Ts 35 nsec 5 nsec 35 nsec 10 nsec 1000 msec 1000 msec 1000 msec 1000 msec 1000Ts 1000Ts 500 psec 200 KHz 200 KHz 16 383 counts 2 25 usec 127 5 nsec 2 nsec 500 psec 127 5 nsec 2 nsec 500 psec Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Miscellaneous Specifications Description Sym Minimum Maximum Trigger Input Voltage Range TRIG IN ov 3 3V 5 0 V max Trigger Input Logic Low Threshold TRIG IN 0 8 V Trigger Input Logic High Threshold TRIG IN 2 0 V Trigger Input Input Impedance TRIG IN 10 Kohm Trigger Input Transition Time TRIG IN 20 nsec Trigger Input Positive Pulse Wid
66. ntrol and Acquisition Software Front Panel 13 Figure 3 Typical DNA Sequencer Getup EE 18 Figure 4 Functional Block Diagram ssssssssseneneeeeeenennnnnns 20 Figure 5 Pipelined Parallel Processor AEN 20 Figure 6 DSP Functional Block Diagram errnnnnnnnnnnvrrrnnnnnnnnnnnnnnrrrnnnnnnnnnnrnnnnnnrnnnenne 21 Figure 7 Intelligent Trigger Module re rone Renten Roh nion meinte 22 Figure 8 MCPC618 Front EE 26 Figure Fo ET EE 27 Figure 10 Data Configuration Dialog Box 36 Figure 11 High Voltage Supply Dialog BOX rrrrrrnnnnnvvrvnnnnnnnnnrnnnnnrrrnnnnnnnnnrrnnnnnrrnnnnen 37 Figure 12 Gain Compensation Dialog Box rrrrrrrnnnnvvrrnnnnnnnnnrnnnnnrrrnnnnnnnnnrrrnnnnrrrnnenne 38 Figure 13 Log File Converter Dialog BOX ee 39 Figure 14 Select File Dialog BOX eech iesst eege dE EEN A0 Figure 15 Band DS elen 41 Figure 16 Flag Definition PSU ex ree tet ex nx nb bn hex x bu tn etu hax etu e tese lie 42 Figure 17 Discriminant Definition Pane ricette puteis ta dade decns 43 Figure 18 Count Record EE 45 8 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com List of Tables Table 1 System Specifications EE 15 Table 2 Trigger and Count Period Specifications rrvrrrrrrrrrrrrrrrrrrrrrnrrrrrrrrrnnnnnnne 16 Table 3 Miscellaneous Specifications nnnneeeeeeeennnnnneeeesertnnnnnnnneeerrnnnnnnnneserennna 17 Table 4 Mechanic
67. onfiguration Spectral filtering operands for flag 3 configuration 48 Parameter Limits 0 Standby Mode 1 Acquire Mode Range 100 13900 10 1390V Range 100 13900 10 1390V Range 0 64 Range 0 64 Range 0 64 Range 0 64 Bit 0 HV Supply 1 Enable Disable Bit 1 HV Supply 2 Enable Disable Range 100 13900 10 1390V Range 100 13900 10 1390V N A 0 65535 0 4095 3 0V full scale Range 0 255 each bit position corresponds to 1 of 8 band enables Range 0 255 1 channel per bit Range 0 255 1 channel per bit Range 0 255 1 channel per bit Range 0 255 each bit position corresponds to a flag enable Flag0Operand0 2 Range 0 32767 Flag0Operand1 3 Range 0 7 or 65535 1 channel per bit or LSB wgt 65535 Same as Above Same as Above Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Index 42 45 46 49 50 53 54 57 58 61 62 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 Parameter Name Flag3OperandO Flag3Operand3 Flag4OperandO Flag4Operand3 Flag5Operand0 Flag5Operand3 Flag6Operand0 Flag6Operand3 Flag7OperandO0 Flag7Operand3 PTerm0 PTerm7 DataFilterEnable ProcessingEnables TimestampEnable DAC_Spare Timestamplnterval CustomWordsEnable EventCustomCount RESERVED
68. ords are generated ee Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Input Trigger Input trigger is used to trigger the count process when incoming data on a specific channel mn J J J M I exceeds a user defined threshold No external trigger signal is required The count period determines the time over which the input signal is accumulated and is typically set to closely match SAMPLE PERIOD tt tt tt tt t1 tt t1 tt t1 T the expected period over which the desired WINDOW mmc dd counts are to be measured The figure at right shows a timing diagram for input triggering RM When using this mode the count period must MTEGRAL 89 always be a multiple of the sample period Ts OVER 7T The count total during the sliding threshold COUNT window which is always equal to the count E a e period is compared to the trigger threshold level In the example the threshold equals three counts the count period equals 7Ts and thus only at one point does the count total over the 7Ts count period exceed the three count threshold The crossing of the threshold triggers the MCPC618 to acquire data across all channels and generate a data record To better position the count period around the desired count activity the actual accumulation window can be shifted by an integer number of Ts intervals
69. orted Table 8 Data Packet Header Word Signal Data Signal data is organized sequentially starting with the count data from the first channel followed by the data from the second channel and so on Individual channels are included in the count record only if they are enabled under the Data Configuration menu Signal channels are formatted as unsigned 16 bit magnitude only words with the LSB for each word located in bit 0 The integer value for each signal channel is equal to the total number of counts accumulated by the system during the count period Range Bits Following the signal data in the record is the bit packed range word that if enabled holds the range reporting bits Disabling the range bit reporting under the Data Configuration menu removes the range words from the count record Out of range OOR and input error ERR bits are formatted as shown in Figure 18 Range bits for unused channels should be ignored Trigger Time Stamp The trigger time stamp is encoded as a two word 32 bit value The least significant word follows the most significant word in the count record For time stamp reporting the trigger time relative to the start of the acquisition the time in the D Text Header is computed by multiplying the time stamp by the time stamp resolution selected in the Data Configuration menu Disabling the reporting enable for this field removes that data from the count record Count Record Length The length L i
70. ph Attributes dual 4 x 4 graphs Attributes dual 8 x 8 graphs Attributes single 16 x16 graph Table 9 User Configuration Table 52 Parameter Limits Bit 0 Graph x flip Bit 1 Graph y flip Bit 2 Graph transpose Bit 6 Graph color BW Bit 0 Graph A x flip Bit 1 Graph A y flip Bit 2 Graph A transpose Bit 3 Graph B x flip Bit 4 Graph B y flip Bit 5 Graph B transpose Bit 6 Graph color BW Bit 0 Graph A x flip Bit 1 Graph A y flip Bit 2 Graph A transpose Bit 3 Graph B x flip Bit 4 Graph B y flip Bit 5 Graph B transpose Bit 6 Graph color BW Bit 0 Graph x flip Bit 1 Graph y flip Bit 2 Graph transpose Bit 6 Graph color BW Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Custom Configuration Table The custom table is a reserved space of 250 words that is used by applications programmers to store custom configuration data 1000 1249 Factory Configuration Table CustomElement0 CustomElement249 16 SHORT Reserved location for custom configuration parameters Table 10 Custom Configuration Table Parameter Limits N A 0 65535 Factory programmed read only configuration data is found in the factory table This table is 750 words long and is described below Index 1250 1251 1252 1253 1254 1509 1510 1765 1766 1767 1768 1769 1770
71. ps is preset such that the signal from a single photon results in an output optimized for the input range of the discriminator The discriminator threshold control voltage Vin in combination with the PMT s high voltage cathode bias are used to set the ideal discrimination point for detecting single photons from the PMT Detected photons are counted by the high speed accumulator during the count period Additionally a programmable microGate function provides another level of gating so that counting can be selectively enabled or disabled synchronously with an external gating trigger signal Unlike the count period which is setup and controlled by the intelligent trigger module this gate operates and is controlled with sub nanosecond resolution The microGate is usually synchronized with an external excitation source like a laser so that counting can be disabled for the short period of time while the source is active Data Acquisition Data acquisition is initiated by a trigger signal either internal or external detected by the MCPC618 s intelligent trigger module Each trigger initiates a count period which starts the accumulation of the photon count signals across all channels The parallel architecture of the counting channel circuitry allows count accumulation to take place simultaneously across all channels thus achieving very high effective count rates At the end of the count period the data is transferred to the Pipelined Parallel Processor throug
72. red The additional triggers are counted to reach the Trigger End Count but not displayed because none of them resulted in the acquisition of a count record GE Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com Trigger End Count A user programmable value that specifies the Trigger Count value that terminates the Acquire period This is normally used in the Log Only acquisition mode where it is set equal to the total number of count records to be acquired In this way the MCPC618 acquires a complete set of count records in its buffer ends its acquisition period and transfers the buffered data to the PC A value of zero for the Trigger End Count corresponds to an infinite acquisition period Trigger Rate Reports the average trigger rate measured over the period of time set in the Gate Time box The reported rate is calculated by taking the total number of triggers seen by the system during the Gate Time and dividing by the Gate Time The Trigger Rate is unaffected by the actual number of records collected by the unit Gate Time The period of time over which the Trigger Rate is calculated Record Count Indicates the running total of the number of records accepted by the MCPC618 and transferred to the PC The counter is cleared when an acquisition period is restarted and will roll over if the maximum record total is reached This counter is also used as an indicator of
73. responds to a flag 0 Disabled 1 Enabled Bit 0 Spectral Filtering Enable Bit 1 Gain Enable Bit 2 Background Subtraction Enable 0 Disabled 1 Enabled 0 4095 3 0V full scale Range 10 100000 10ns per bit 0 Disabled 1 Enabled Range 0 64 1 word per bit N A 0 65535 0 Particle 1 Image Range 1 8191 Range 0 256 1 channel per bit 0 Disabled 1 Enabled Bit 0 Cross Bank Enable Bit 1 Bank 1 Main Trigger Bit 2 Bank 2 Main Trigger Bit 3 Bank 3 Main Trigger Bit 4 Bank 4 Main Trigger 0 Real Time Acquisition no packing 1 High Speed Acquisition 0 GP Output Disabled 1 GP Output Always On 2 GP Output Linked to Spectral Filter Match Range 10 200000 0 1 2000us Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Index 88 89 90 91 92 99 100 103 104 111 112 119 120 127 128 129 130 131 132 133 135 136 137 138 139 142 143 149 150 405 406 661 662 677 678 Parameter Name GPOutputPeriod IntBoxcarEnable BoxcarWidthEnable ResetDelay0 ResetDelay3 TrigSource0 TrigSource3 TrigPeriod0 TrigPeriod3 IntegPeriod0 IntegPeriod3 IntegDelay0 IntegDelay3 SibSel0 SibSel1 SibSel2 SibSel3 SibSel4 SibSel5 SibSel7 TriggerEndCount TrigS
74. s or imported into a database program for further processing For details on the data format of binary and text log files the Log Files section of this manual should be consulted When the Log File Converter utility is selected the dialog box shown in Figure 13 opens Here the user selects the source binary file log that is to be converted into a text file txt by pressing the Select File button This in turn opens the dialog box shown in Figure 14 where the user then browses to the source file The target file is the name of the text file that results from the conversion of the source binary file Similar in behavior to the source file select button a dialog box opens where the user browses to the target directory and names the target file Once both the source and target files are selected the converter is initiated by pressing the Convert button The progress of the log file conversion process is monitored by observing the Progress bar at the top of the dialog box amp Log File Converter Convert Binary File to TextFile Progress lon n6 Status Select a Source Log File and Target Text File and press Convert SE E cone Figure 13 Log File Converter Dialog Box 39 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System Save in Gi My Documents v 3 2 Er O anayzer Projects Downloaded
75. s or other rights of third parties which may result from its use No license is granted by implication or otherwise under the patent and proprietary information rights of Vertilon Corporation 2012 Vertilon Corporation ALL RIGHTS RESERVED UM6180 1 1 Feb 2012 64 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com
76. seconds Specifies the time to collect Events The function will return after the specified time has elapsed Set to zero to collect for an indefinite length of time Used to select the acquisition mode False should be entered if the returned event data is to be immediately displayed True should be entered if large amounts of data are to be collected before being processed by another window thread or logged to disk Accepts a standard LabVIEW error cluster Data collection is not performed if an error is present Set to True if the data is to be messaged to another window Set to False if messaging is not used If True the MessagingArray must be configured When enabled the Data Interface will call the Windows API function PostMessage indicating to the specified window thread using the specified message that data is available to be processed The wParam argument of the message will indicate which of the two specified buffers has been filled and the IParam of the message will indicate the length of the data within that buffer At the beginning of the data buffer are two 32 bit integers representing the running total counts of events and triggers received respectively Both values are stored little endian The remainder of the buffer contains event data length IParam 4 Contains the information required for messaging Element 0 The handle of the window to be messaged Element 1 The message to be sent to the specified windo
77. tampSelect DataFormat0 DataFormat3 RESERVED ChOGainComp Ch255GainComp ChOTrigThresh Ch255TrigThresh ChOTrigEnb Ch255TrigEnb MBandEnables Type 32 LONG 16 SHORT 16 SHORT 32 LONG 16 SHORT 32 LONG 32 LONG 32 LONG 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 32 LONG 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT 16 SHORT Description Period of general purpose output Enables Disables boxcar mode Enables Disables boxcar width output Unused reset delays 1 through 4 Trigger source bank 1 to 4 Trigger period bank 1 to 4 Integration period bank 1 to 4 Integration delay bank 1 to 4 Hamamatsu R5900U L16 Hamamatsu H8711 Pacific Silicon Sensor AD LA 16 9 DIL18 Hamamatsu H7260 Undefined Reserved for SIB expansion Number of Triggers allowed in Acquire mode Triggerstamp Enable Bank 1 to 4 data format Reserved for expansion Gain compensation values for each channel Input triggering threshold values for each channel Input triggering enables bit packed for each channel Matrix filtering band enables 50 Parameter Limits Range 10 200000 0 1 2000us 0 Disabled 1 Enabled 0 Disabled 1 Enabled N A 0 65535 0 External Edge Trigger 1 Internal Trigger 2 Level Trigger 3 Input Trigger 4 DSP Trigger Cross bank use only 5 Pre trigger Range 500 10000000 200kHz 10Hz Range 5 100000
78. tation trigger is an external 2 ss Cs signal that may for example be used to drive an excitation source like a laser When fired a large amount of unwanted signal is generated that should be excluded from the count total This is shown in the figure as five closely spaced pulses immediately following the excitation trigger Without the microGate the count total would be nine counts over the count period Because the unwanted energy occurs over a very short time on the order of nanoseconds control over the exact position of the count period using the edge trigger signal would be too imprecise and likely result in the exclusion of some desired counts Using the microGate which by its design ties directly to the count accumulator in the front end counting channels the counting process can be momentarily stopped so that the unwanted pulses can effectively be excluded from the count total This is done by using the excitation trigger as the input to the microGate generator The result is just four counts when using the microGate The MCPC618 gives the user full control over the microGate The gate width tmgow and delay from trigger tmga as well as the trigger edge polarity and gate polarity are easily configured in the user interface gt Le COUNT i PERIOD 25 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting Sys
79. tem Hardware Interface The photo below shows the front panel connectors and status indicators on the PhotoniQ MCPC618 9 H EiVertilon Figure 8 MCPC618 Front Panel 1 Main Power Switch Lighted main power switch 2 Trigger Input BNC Main trigger input A positive edge on this input initiates the count period which when complete generates a record of count data for all channels 3 Trigger Indicator Green LED Indicates when a trigger is supplied to the unit on the Trigger Input connector 4 Trigger Output BNC Main trigger output When in edge level or internal trigger mode the output from this connector is the actual count period used by the MCPC618 to accumulate photon counts on its inputs In input trigger or pre trigger modes the trigger output indicates the trigger point shifted by the programmable delay time 5 Acquisition Indicator Green LED Indicates when a count record is generated by the unit 6 Threshold Input BNC Analog voltage input used to indirectly control the threshold to the discriminators Bypassed when using the internal threshold generator 7 microGate Input BNC The trigger input to the microGate generator 8 microGate Output BNC Output from the microGate generator used to align gating with input signals 9 Input Channels BNC Photon counting input channels total of eight 26 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 F
80. th TRIG IN 100 nsec Trigger Input Negative Pulse Width TRIG IN 100 nsec Trigger Output Voltage Range TRIG OUT ov 3 3 V microGate Input Voltage Range GATE IN ov 3 3 V 5 0 V max microGate Input Logic Low Threshold GATE IN 0 8 V microGate Input Logic High Threshold GATE IN 2 0 V microGate Input Input Impedance GATE IN 50 ohm microGate Input Transition Time GATE IN 20 nsec microGate Input Positive Pulse Width GATE IN 10 nsec microGate Input Negative Pulse Width GATE IN 10 nsec microGate Output Voltage Range GATE OUT ON 5 0V External Threshold Input Voltage Range THRESHOLD 0V 5 0 V External Threshold Gain THRESHOLD 20 mV per V External Threshold Input Impedance THRESHOLD 500 Kohm Trigger Stamp Counter Range 0 232 1 Time Stamp Counter Range 0 232 1 Time Stamp Resolution Decade Steps 100 nsec 1 msec Time Stamp Maximum Decade Steps 429 4967 sec 49 71026 days Trigger Counter Range 0 108 Table 3 Miscellaneous Specifications Mechanical Specifications Description Specification Width 9 843 in 250 mm Height 3 346 in 85 mm Depth 10 236 in 260 mm Table 4 Mechanical Specifications PC System Requirements e Microsoft Windows XP operating system e Intel USB 2 0 high speed host controller with 82801Dx chipset low speed is not supported e Run time engine for LabView version 9 0 for use with DLLs lt 17 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010
81. w Element 2 A pointer to the first of two A 1MByte buffers Element 3 A pointer to the second of two B 1MByte buffers Element 4 A pointer to an unsigned 16 bit integer Acquisition will stop if the referenced value is zero when either a message is sent or an internal timeout is reached Length of MessagingArray array Returns the number of events read by the Data Interface Duplicate file refnum output Boolean output returns True if the Data Interface returned as a result of reaching the number of events specified by NumEvents Boolean output retums True if the Data Interface returned as a result of reaching the timeout specified by TimeoutS Boolean output returns True if the Data Interface returned as a result of reaching the time to collect specified by TimeToCollectS 57 Vertilon Corporation 66 Tadmuck Road Westford MA 01886 Tel 978 692 7070 Fax 978 692 7010 www vertilon com MCPC618 Multichannel Photon Counting System ImmediateEventData Returns a portion of the collect Event Data This output is only guaranteed to be valid when NumEvents is set to 1 and NumEventsReached is True The value of this output is unspecified when the Data Interface returns due to a timeout or a count larger than 1 To evaluate all data use file logging or messaging len2 Length of ImmediateEventData array ElapsedTimeS Returns the time elapsed while collecting data errorOut Points to error information

MCPC618 Multichannel Photon Counting System

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