Radiant v2.0 User Guide
Contents
1. Completing the Opal Kelly FrontPanel USB Driver Setup Opal Kelly FrontPanel USB Driver has been installed on your computer Click Finish to close this wizard Visit the Opal Kelly website for the latest news and support 10 10 Sentinel System Drivers will install for the license key In some cases Windows XP may need to restart before it can install these drivers Sentinel System Driver Installer 7 5 8 A Please wait while Windows configures Sentinel System Driver Installer ay 7 9 8 InstallShield Wizard Completed 3 i InstallShield Wizard Completed The Instalishedid wizard Fas Successsulhy inctaked Peon The InstallShield Wizard has successfully installed Plexon Radiant Version 2 Windows sG Olek Finish to exit the wired Radiant Version 2 Windows x64 Click Finish to exit the wizard Show the Windows Installer log Cancel 12 Restart the computer to complete the installation 4 Plexon Radiant Version 2 Windows x64 Installer Infor W Plexon Radiant Version 2 Windows x86 In You must restart your system for the configuration changes made to Plexon Radiant Version 2 Windows x64 to take effect Click Yes to restart now or No if you plan to restart later YOU Must restart your system for the configuration changes made to Flexon Radiant Version 2 Windows 86 to take effect Click Yes to restart mow or No if yau plan to restart later te PlexBright 4 Channel Optogenetic2. r s Opal Kelly FrontPanel USB Driver Setup Se A 3 License Agreement yS gt f gt eae O p a K c ly Please review the license terms before installing Opal Kelly FrontPanel USB Driver Press Page Down to see the rest of the agreement rontPanel License Agreement Copyright 2005 2011 Opal Kelly Incorporated IMPORTANT The product including the software firmware and other electronic data contained within the product is licensed not sold and available for use only under the terms of this license agreement By dicking the I Agree button below and continuing with the installation you are consenting to be bound by this license agreement If you do not agree to the terms of the license agreement click the CANCEL button If you do not agree to the terms of this license agreement and wish to return this product please contact us at support opalkelly com If you accept the terms of the agreement click I Agree to continue You must accept the agreement to install Opal Kelly FrontPanel USB Driver Opal Kelly Incorporated 9 Click Finish When the Opal Kelly Driver Setup is complete Completing the Opal Kelly FrontPanel USB Driver Setup Opal Kelly FrontPanel USB Driver has been installed on your computer Click Finish to close this wizard Visit the Opal Kelly website For the latest news and support Cancel Radiant Version 2 0 E Opal Kelly FrontPanel USB Driver Setup
3. 0 19 As before if the Start digital input goes low in the middle of a pattern the pattern will be cut short A start signal that occurs after a pattern has been cut short will resume playback of the pattern When a stimulation pattern plays to completion the Radiant software will automatically re load the pattern in preparation for another start signal However the process of detecting the end of the pattern and re loading the pattern takes some time and it may be on the order of 100 ms before the pattern is ready to be triggered again There are two ways to set the polarity of the digital inputs Positive and Negative These can be accessed from the drop down menu DI Polarity at the top of the screen Info E Digital Inputs DI Mode Edge DI Polarity Positive i Channel 1 Positive Stimulation Status In Positive mode the controller responds to a rising edge 1f the inputs are in edge mode or to a logic 1 in level mode This is the more common method of communicating through TTL In Negative mode the controller responds to a falling edge if the inputs are in edge mode or to a logic 0 in level mode Care should be taken when changing polarity as it is possible to inadvertently start stimulation For example the digital inputs are pulled low so if the polarity is set to negative with no input connected all channels that are ready will start when the polarity is changed from positive to negative 6 6 Defining a Pattern with a t
4. G button Radiant Version 2 0 25 A constant primitive has a value and duration The value can be set to 0 to create a pause or to a non zero value to hold an output Value ee pene s Duration A pulse primitive has a value pulse width period or frequency and number of repetitions or total duration The value is the amplitude Its units correspond to the units set for the pattern The pulse width is the amount of time that the pulse holds the value The period is the total time of the pulse on off The repetitions field sets how many times the pulse 1s repeated Note that period can also be defined in terms of frequency and repetitions can also be defined in terms of total duration Width m Value J Repetitions 5 aamin amie Period At any time you can click on the pattern group or primitive and visually see what you have defined by looking at the Pattern Generator Visualizations window The display automatically changes to show what is selected in the Pattern Generator window The pattern is selected in the image below Pattern Generator n E R A 20x Units LED mA Repetitions Count 1 Group 1 Period Frequency Period Period ms 300 Sa aa a a Repetitions Count 2 A isore ainil f V y J 1 Value mA 15 T Pulse Constant Ramp Period Freq Period Reps 3 Reps Period ms 50 k si Pulse Width ms 25 Ph HI Group ti Total Duration Repe Repetitions C
5. TTL compatible digital inputs for each channel Inputs should range from 0 5 V Inputs lt 0 8V are logic 0 and gt 2 0V are logic 1 The pinout for the Digital In connector is shown below 6nd 17 Gnd i5 NC 13 Start 4 S5tart3 NE Ne 19 a i am 5 Start 2 me 1 Start 1 Digital Out Digital In e On a Power In a a E Er B E ae FE EF nn b N of mF b N O i oozu zu g KS 3 3 D OO D 5 2 amp ot nm HH aA Es fad The digital inputs can be operated in two different modes To select the mode click the drop down menu next to DI Mode at the top of the screen DI Mode DI Polarity Edge ual ee The first mode is called Edge mode In this case a rising edge on the start pin initiates stimulation and a rising edge on the corresponding stop pin stops stimulation If the stop input comes in the middle of a stimulation pattern the pattern will be cut short Stimulation will also stop if the pattern plays to completion This is illustrated below Current Voltage Output On Start Edge Trigger Mode Stop Edge Trigger Mode The second mode is called Level mode In this mode the stimulation pattern starts when the Start input transitions from 0 to 1 and continues until the pattern is complete or the Start input transitions from 1 to 0 This is illustrated below Current Voltage Output On Start Level Trigger Mode Radiant Version 2
6. by ec international treaties Plexon Inc Dallas TX If the software is already installed then the welcome screen gives you the option to repair re install or remove the software as shown on the right above If the software is not already installed then the welcome screen shown above on the left appears Click Next to continue 4 Read and accept the Plexon End User License Agreement Click Next to Continue r P poeem x x 3 Plexon Radiant Version 2 Windows x86 InstallShield Wizard gt E Plexon Radiant Version 2 Windows x64 InstallShield Wizard License Agreermenl YOU MAY NOT SELL RENT LEASE OR SUBLICENSE THIS SO YOU MAY MOT SELL RENT LEASE OR SUBLICENSE THIS Please read the following license agreement careft SOFTWARE Please read the following license agre Plexon End User License Agreement Plexon End User License Agreement FLEXOA INC SOFTWARE END USER LICENSE AGREEMENT PLEXON INC SOFTWARE END USER LICENSE AGREEMENT IMPORTANT THIS SOFTWARE END USER LICENSE AGREEMENT EULA 15 A LEGAL AGREEMENT BET WEDN YOU AND PLEXOH INC READ IT CAREFULLY DEFORE USING THE SOF TWiRE IT PROVIDES LICGNSE TO USE THE SORT WARE AND CONT ANS WARRANTY INFORMATION AND LIABILITY DISCLAIMERS BY SELECTING THE DOWLOAD OF INSTALL WOW BUTTON AND USING THE SOFTWARE YOU ARE CONFIRMING YOUR ACCERTANCE OF THE SOFTWARE AND AGREEING TO RECOME BOUND BY THE TERMS GF THIS AGREEMENT IF YOU DO NOT AGREE TO BE BOUND BY
7. left the output voltage will change to 1V and the green triangle will change to a red square Controller 1 Xon Controller 1 m4 PPP bib wapp Ready mif Ch 1 Ginfe E Digital Inputs i E Digital Inputs to Start pimode Edge Playing __ DI Polarity Positive E Channel 1 E Channel 1 Once the channel is running you can adjust the slider to change the output voltage The output will remain at the set voltage until you stop the channel or change the value 6 5 Starting and Stopping Stimulation There are two ways to start playing a stimulation pattern with the host PC through software and with a hardware digital input Note that a pulse started from software can be stopped with a digital input and vice versa In software until a pattern for a channel has been defined the start button in the top left of the interface is grayed out As soon as the pattern has been selected the start button becomes active At that point the channel can be triggered to start To initialize stimulation from the software you can click the start button for the individual channel Alternatively the start all button to the right of channel 4 s start button can be clicked and every channel of that controller that has been configured will start at the same time Once clicked the start button changes functionality to become a stop button PlexBright 4 Channel Optogenetic Controller with Radiant In hardware there are start and stop
8. possible that your layout has changed Go to Window Layout Reset to Default Layout Radiant software requires a USB license key to be operated If the key is not plugged into the computer you will see the following warning Radiant cannot obtain a valid license Radiant can not be started For purchasing information or technical support please contact Plexon Inc Phone 214 369 4957 Fax 214 369 1775 EMail info plexoninc com 6 4 Using Manual Mode to Operate the Controller The fastest and easiest way to use the controller is in manual mode This is the mode that the software will start in by default the first time the software is run If it is not in manual mode you can select it by clicking the drop down menu next to Pattern Source Stimulation Status Output Device LED Type Blue 465 nm 300 mA fixed Output Mode Current mA E e a Manual Wo de I Ciment Output mA Stimulation Status Pattern Generator ak e ma ma Radiant Version 2 0 17 Begin by using a BNC cable to connect the output marked CH 1 to an oscilloscope Next set the Output Device to Laser and the Voltage Limit to 5000mV Then click in the box below Manual Mode to and move the slider to 1000 mV TIP Fine tuning the slider value Fine incremental changes can be made to the slider value using the up and down arrow keys or the scroll wheel on your mouse When you press the start button for channel 1 in the upper
9. terms before installing Opal Kelly FrontPanel USB Driver Press Page Down to see the rest of the agreement FrontPanel License Agreement Copyright 2005 2011 Opal Kelly Incorporated IMPORTANT The product including the software Firmware and other electronic data contained within the product is licensed not sold and available for use only under the terms of this license agreement By clicking the I Agree button below and continuing with the installation you are consenting to be bound by this license agreement If you do not agree to the terms of the license agreement click the CANCEL button IF you do not agree to the terms of this license agreement and wish to return this product please contact us at support opalkelly com If you accept the terms of the agreement click I Agree to continue You must accept the agreement to install Opal Kelly FrontPanel USB Driver Opal Kelly Incorporated Opal Kelly FrontPanel USB Driver Setup Welcome to the Opal Kelly FrontPanel USB Driver Setup This wizard will guide you through the installation of the FrontPanel Driver only package We suggest you uninstall any previous versions of FrontPanel and make sure that FrontPanel is not currently running It is also recommended that you close all other applications before continuing This will make it possible to update relevant system files without having to reboot your computer Click Next to continue
10. than 4us lt 200ns 4s Digital Input i i Trigger i Digital Output Status Indicators Non Zero Stop l l Zero l I ESS a a Voltage l Output Because all channels operate on the same 10kHz clock there is 100us of variation in start latency when starting a second channel while the first is running This is because the second channel cannot start until a rising edge of the 1O0kHz clock is seen and this clock is asynchronous to the digital input Digital stop timing in voltage mode is shown below All digital outputs change to reflect that a digital input stop trigger has been detected within 200ns though not exactly at the same time The voltage output begins to fall within 110us just over one sampling period after the digital input trigger and the fall time of the voltage output is less than 9us PlexBright 4 Channel Optogenetic Controller with Radiant 1 50ns lt Ous lt 110us Not to Seale gt Digital Input Trigger Digital Output Status Indicators Ruming Non ero Stop Zer Voltage Output In current mode there are additional delays associated with the current generation circuit and the LED turning on 300mA and 1000mA LEDs were tested at 100mA and their respective maximum currents The worst case observed time between the digital input and the light reaching 90 of its maximum was 320us The rise time of the light output from 10 to 90 was up
11. to 180us and the fall time was up to 300us In general rise times were actually shorter for higher currents Additionally orange modules had shorter rise times than blue modules Fall times were approximately the same across different the current values for a given module The blue modules had longer fall times than orange ones These delays are still small relative to the kinetics of the opsins that are available at this time 10 2 Synchronization of Multiple Controllers The Radiant GUI supports connecting up to four controllers to a single computer It should be noted that each controller has its own internal clock and this will result in slight timing variations between Controllers This variation will be most noticeable during very long stimulation protocols The crystal that generates the clock has 50 ppm stability In the worst case if your pattern is an hour long it could potentially finish 0 36 seconds earlier or later than the same pattern running on a second controller that was started at the same time assuming one clock is 50 ppm fast and one is 50 ppm slow This is a worst case scenario but there will certainly be some drift between different controllers If you have a long pattern that actually consists of smaller repeated pattern the best option is to define a pattern consisting of just one basic unit and then use a digital input to trigger all of the controllers at the same time As an example rather than repeating a se
12. 51 A Windows XP Windows 7 USB 2 0 Operating Systems Interface Analog outputs Stimulation mode Voltage or Current Number of analog output channels Maximum Current Current Mode 1100 mA U1 lt Maximum Voltage Voltage Mode Resolution for setting Max Current or Voltage 4 3mA 19 5mV Output Resolution Max current or voltage 256 Output accuracy with max set to 5V for outputs lt 800 mV error lt 18 mV for outputs gt 800 mV error lt 2 Output accuracy with max set to 1000mA 2 SD 3 9mA 4 0mA Temporal resolution 100 us Output rise time Voltage Mode lt 4 us 0 5V 1 MO 11 5 pF load Minimum LED light pulse width 500 us amplitude dependent Digital inputs outputs Number of digital inputs 8 2 per channel Number of digital outputs 16 4 per channel Tetreres o maywan O Radiant Version 2 0 4 42 PlexBright 4 Channel Optogenetic Controller with Radiant PlexBright 4 Channel Optogenetic Controller with Radiant Software Part of the PlexBright Optogenetic Stimulation System User Guide Plexon Inc 6500 Greenville Avenue Suite 700 Dallas Texas 75206 Telephone 1 214 369 4957 Fax 1 214 369 1775 www plexon com Copyright 1983 2014 Plexon Inc All Rights Reserved Plexon Inc Proprietary Document number OPTMNO002b Document status Standard Software version Radiant Version 2 0 Date April 2014 r LEXON
13. Controller with Radiant 5 2 Hardware Installation Read the entire Hardware Installation section before proceeding with any of the Steps An oscilloscope is highly recommended for viewing the output of the channels in voltage mode 1 Connect the AC power cord between the AC outlet and the power supply 2 Connect the DC power cord between the power supply and the PlexBright 4 Channel Optogenetic Controller 3 Connect the USB cable between the PlexBright 4 Channel Optogenetic Controller and the computer Optogenetic Controller 4 Connect the BNC cable between the PlexBright 4 Channel Optogenetic Controller channel 1 and the Oscilloscope Optogenetic Controller PLEXON Radiant Version 2 0 11 12 5 3 Turning on the Power for the First Time 1 Flip the power switch to the on position The LED next to the switch on the end of the PlexBright 4 Channel Optogenetic Controller should illuminate The first time you turn the stimulator on a Found New Hardware balloon will appear in the lower right hand corner of the computer screen In Windows 7 the balloon changes from Installing device driver software to Opal Kelly XEM6001 In Windows XP the Found New Hardware Wizard will appear Choose Install the software automatically After it is finished the balloon will change to Your new hardware is installed and ready to use The following diagram shows the dialog boxes for this process PlexBrig
14. IMPORTANT THIS SOFTWARE END USER LICENSE AGREEMENT EULA IS A LEGAL AGREEMENT BETWEEN YOU AND PLEXON INC READ IT CAREFULLY BEFORE USING THE SOFTWARE IT PROVIDES A LICENSE TO USE THE SOFTWARE AND CONTAINS WARRANTY I accept the terms in the license agreement OL geoepe the berms in the kerge agreement Gildo nota the terms in the botret agreement Back 5 You will be asked to confirm where the files will be stored Click Next to Continue P r R r x i Plexon Radiant Version 2 Windows x86 InstallShield Wizard J Plexon Radiant Version 2 Windows x64 InstallShield Wizard x Ready to Install the Program Ready to Install the Program The witerd amp ready bo begr initaletion The wizard is ready to begin installation TP you want fo review or change any of your installation settings cick Back Cikk Cancel to eet the ward If you want to review or change any of your installation settings click Back Click Cancel to exit the wizard Current Settings Setup Type Custom Destination Folder C Program Fles elexon Inc packanity 2564 C Program Files x86 Plexon Inc Radiant V2 x64 Leer information Mame 909 Comeau User Information Name andyq Company Microsoft __ lt Beck J __instat_ __concel 6 When the installation begins it may take a couple of minutes before the progress bar begins moving across the window This is especially true in Windows 7 P A 5 i E i Pl
15. al Dw I Neurotechnology Research Systems PlexBright 4 Channel Optogenetic Controller with Radiant Software Part of the PlexBright Optogenetic Stimulation System Radiant Version 2 0 April 2014 User Guide Plexon Inc 6500 Greenville Avenue Suite 700 Dallas Texas 75206 USA CAUTION THIS OPTOGENETIC CONTROLLER IS NOT FOR USE IN HUMANS CAUTION READ THE ENTIRE MANUAL BEFORE ATTEMPTING TO OPERATE THIS EQUIPMENT CAUTION In LED mode the output of the PlexBright 4 Channel Optogenetic Controller can range up to 12V This high compliance voltage makes it possible to drive several LEDs in series from one controller output channel Some external devices may be damaged by the voltages the controller can generate Exercise caution when connecting the PlexBright 4 Channel Optogenetic Controller output to other devices to avoid inadvertently applying voltages up to 12V to those devices CAUTION The output of some PlexBright 4 Channel Optogenetic Controllers can temporarily go to 12V when the Radiant software is launched Specifically this glitch occurs when revision blank controllers built prior to Nov 21st 2013 PN 08 06 A 04 are switched from Laser mode voltage output to LED mode current output When the Radiant software starts up the controller always switches from Laser mode to LED mode Thus the glitch to 12V always occurs in revision blank controllers when the Radiant software is launched You can
16. annel will have no effect until the status changes to Ready 7 2 Output Device There are two choices in the drop down menu for Output Device e LED e LASER Whenever LED 1s selected the controller will output a current Selecting Laser will output a voltage This voltage is intended to be used to control a laser with an analog control signal 7 3 Output Mode When the output device is set to LED the output mode can be set to either Current mA or Power mW If Power is selected the power delivered at the Current Output Limit must be manually entered This power setting should be based on a light measurement of the entire optical system as described in Section 6 9 Using Different Scaling Options on page 23 Note that when using one of the pre configured Plexon LED modules the current limit is set automatically and is shown next to the wavelength in the LED Type window When the output device is set to Laser the output mode can be either Voltage mV or Power mW If Power is selected the power delivered at the Voltage Output Limit must be entered 7 4 Current or Voltage Limit In current mode the PlexBright 4 Channel Optogenetic Controller can output more current than some LEDs can handle To reduce the risk of damaging your LED the current limit can be set If one of the default LED types is selected from the dropdown list the current limit for that LED type is set automatically If the custom LED type is selected yo
17. cond pattern 3600 times in software or a file you Radiant Version 2 0 39 40 could define the second pattern set the number of repetitions to 1 and trigger all of the controllers with the same 1Hz digital input This will prevent drift between Controllers 10 3 LED Flashes When Changing from Voltage to Current Mode In controllers built before Nov 21st 2013 08 06 A 04 if an LED is connected to the controller while it is in voltage mode and the output is switched to current mode a brief pulse of light approximately 4ms long will occur as the current output stabilizes at zero The easiest way to avoid this is to switch to current mode before connecting the LED to the controller PlexBright 4 Channel Optogenetic Controllers manufactured prior to Nov 21st 2013 are eligible for a no charge upgrade to revision A You can determine the revision of the controller by looking at the label on the bottom of the controller or by looking in the Radiant software under the info section for each controller Radiant version 2 0 and later Please see the caution on the inside front cover Contact Plexon support 1 214 369 4957 or support plexon com for additional information PlexBright 4 Channel Optogenetic Controller with Radiant 11 Specifications Dimensions power supply 5 8 in x 1 3 in x 2 4 in controller 8 0 in x 1 9 in x 3 8 in Weight power supply 1 0 lbs controller 1 1 lbs Power requirements 100 240 VAC 47 63 Hz 0 96 0
18. d permanently damage your LED and will not show anything useful on your oscilloscope e Try to connect two channels to one LED 8 5 Status LEDs There are 16 status LEDs above the output BNC connectors Device The Device LED indicates which controller number each box is when multiple controllers are connected to a single computer This is necessary to know which unit is being configured when multiple tabs appear in the GUI The LED above 1 will illuminate for controller number 1 the LED above 2 will illuminate for controller number 2 etc I on V off Each channel has a dedicated LED to indicate if it is configured for current output or voltage output If the channel is configured for current output the LED will be illuminated If it is configured for voltage output it will be off Running Each channel has a dedicated LED to indicate if a pattern is running It will stay illuminated for the duration of the pattern even if the pattern specifies a zero level output It mirrors the output on the running digital output pin Non Zero The non zero LED mirrors the function of the non zero digital output Note that for rapidly pulsing patterns it may not be possible to visually distinguish when the output is non zero and when it is zero by looking at the non zero LED If this is the case connect the non zero digital output to an oscilloscope or other electronic recording device Radiant Version 2 0 35 8 6 Digital Ou
19. determine the revision of the controller by looking at the label on the bottom of the controller or by looking in the Radiant 2 0 software under the info section for each controller The Radiant software will also provide a warning dialog on startup if the affected hardware is detected This glitch has been eliminated from PlexBright 4 Channel Optogenetic Controllers starting with revision A PN 08 06 A 04 A If you have a revision blank controller you may return it to Plexon for modification to revision A Contact Plexon support at 1 214 369 4957 or support plexon com for information and instructions PlexBright 4 Channel Optogenetic Controller with Radiant Software Part of the PlexBright Optogenetic Stimulation System User Guide Document number OPTMNO002b Document status Standard Software version Radiant Version 2 0 Date April 2014 Copyright 1983 2014 Plexon Inc All Rights Reserved Printed in the United States of America Plexon Inc Proprietary The information contained herein is the property of Plexon Inc and it is proprietary and restricted solely to assist Plexon Inc customers Neither this document nor the contents may be disclosed copied revealed or used in whole or in part for any other purpose without the prior written permission of Plexon Inc This document must be returned upon request of Plexon Inc Information is subject to change without notice Plexon Inc reserves the right to make changes in equipment d
20. e You will see that it is 2 5V SV 20mW 40mW 6 10 Defining Patterns with the Pattern Generator The most common patterns used in optogenetic experiments can easily be defined with the Pattern Generator This is a built in tool that is used to create hierarchical patterns consisting of constant values square pulses and ramp pulses These can be individually repeated or included in a group that is repeated PlexBright 4 Channel Optogenetic Controller with Radiant The first step in defining a pattern is to create the pattern with the P button Multiple patterns can be defined with the pattern generator but only one pattern can be assigned to a channel at a time Units LED mA Repetitions Count 1 The pattern has associated units and a number of repetitions The units of the pattern must match the units of the channel that you want to assign it to A pattern must have a minimum of one group To add a group click the G button x Di a P n j ae A gr I SAH LFU mT pe Period Frequency Period ms Repetitions Count Groups have an associated period or frequency and number of repetitions The period is specified in milliseconds or it can be specified as a frequency expressed in Hertz A group must have a minimum of one primitive A primitive can be a constant value a square pulse or a ramp pulse These can each be added by clicking one of the three buttons to the right of the
21. e controller from the inner contact and returns to the controller on the outer contact Care must be taken when connecting the outputs in current mode Below are a list of Do s and Don ts to consider when making these connections Do e Make sure that the outside of the BNC connector is not touching anything The cables that came with your PlexBright 4 Channel Optogenetic Controller are insulated for this reason e If you want to drive multiple LEDs with one channel make sure they are connected in series NOT in parallel This can be done with a Plexon Series Y BNC Cable e Set the appropriate current limit for your LED This is done by selecting your Plexon LED type in the GUI or by making a custom LED type and setting the max current in the GUI PlexBright 4 Channel Optogenetic Controller with Radiant Don t e Connect the signal to an oscilloscope You will not damage the controller or the oscilloscope assuming your oscilloscope can handle a 12V input but you will not see any useful signal e Connect to any instrument that cannot handle a 12V input If you plan to use a voltage to control another instrument switch the controller to voltage mode before making the connection to reduce the risk of damaging other equipment e Use a standard BNC T connector to try and split the signal between two LEDs e Use a standard BNC T connector to try to split the signal between an LED and an oscilloscope This coul
22. e Output Device for the channel is Laser and the Output Mode is Voltage the file values will be interpreted as voltage in mY After you select the file the channel is ready and the start button becomes active A preview will appear on the left The pattern stored in the file can be repeated a finite number of times or repeated continuously This is set in the fields below Load from File Note that if the pattern 1s repeated the first value in the file will immediately follow the last value with no gap If your pattern defines a series of pulses you will probably want to add some zeros at the end the pattern to maintain a constant pulse rate during repeated playback For this example select Continuous Channel 1 Stimulation Status Ready Output Device LASER Output Mode Voltage mV Voltage Output Limit mV 5000 Patter Source File Load from File C AUsersiandyg plexoninc Desktopi Patternl_pulse_burGs Repetitions Finite Count 1 After the file has been loaded start the channel by clicking the start button at the top of the GUI 6 8 Verifying the Output on an Oscilloscope Before connecting the output to an oscilloscope confirm that the channel is in voltage output mode There are two ways to do this The GUI should say Output Device Laser Output Mode Voltage mV and the I on V off LED on the controller end panel should be off indicating voltage output PlexBright 4 Channel Optogenetic Controll
23. e first followed by the hardware Do not connect the controller to an implant until you have read the entire manual 5 1 Software Installation Read the entire Software Installation section before proceeding with any of the Steps 1 f W User Account Control Browse to the PLEXON_SW RadiantV2 x64 or x86 folder on the USB flash drive depending on if you have a 64 or 32 bit operating system Right click the file Radiantx86V2Setup exe or Radiantx64V2Setup exe and select Run as Administrator to begin the installation process On some computers you will get a warning jo you want to allow the following program to make changes to this computer Program name RadiantbO4V2 Setup bed Verified publisher Plexon Inc File origin Network drive v Show details Xes pms 3 Click Yes or Run to continue The welcome screen appears W Plexon Radiant Version 2 Windows x86 InstallShield Wizard EJ Radiant Version 2 0 Welcome to the InstallShield Wire Pleson Radlant Vershon 2 Window The IrstaishiedtiR Wirard wil metal Piece WNW BE On Your OOM puna To canmi WARMING This program E protected b T ntematona meane Pesan inc Dalas TAE JE Plexon Radiant Version 2 Windows x64 InstallShield Wizard Welcome to the InstallShield Wi Plexon Radiant Version 2 di The InstallShield R Wizard will install Plexe Windows x64 on your computer To contin WARNING This program is protected
24. er with Radiant TIP The channel might be in current mode If the channel is not playing but the voltage is still 1 25V the channel is in current mode In current mode the LED 1s held at a voltage just below the turn on threshold so that it can be turned on more quickly when stim ulation starts The output for the Patternl_pulse_bursts txt file that was loaded in LASER mV mode should look like the image below Note that the scaling is 100mV per division and 1 second per division gt w 100mYy 6 9 Using Different Scaling Options The PlexBright 4 Channel Optogenetic Controller fundamentally outputs either a 0 5V signal to control a laser or a O 1100mA signal to directly power an LED The Radiant software allows patterns to be specified in voltage or current but some users may prefer to specify their stimulation pattern in terms of light output By inputting the amount of light produced at the maximum current a linear interpolation is performed The software then calculates the amount of current or voltage required to generate any light output As an example if a given LED outputs 1OmW of light at 300mA and the user requests 7mW the software can automatically calculate that 300 mA 7mW 10mW 210 mA of current is required This calculation is transparent to the user Radiant Version 2 0 23 24 12 User Input jn Max Current 300 mA 10 mee ee ee ee em er er er ere eee ee Light 10 mW User Request
25. esign or components as progress in engineering or manufacturing may warrant PLEXON the five line symbol Plexon Inc CereStage CinePlex DigiAmp MiniDigi Offline Sorter OmniPlex PL2 PlexBright PlexDrive PlexStim Radiant RapidGrid and the Plexon logo are trademarks of Plexon Inc Dallas Texas USA Other product and company names mentioned are trademarks of their respective owners li Plexon Inc Radiant Version 2 0 Plexon Inc lil Publication History April 2014 In Section 1 added hardware part number 08 06 A 04 C In Section 11 changed voltage output accuracy from 10mV 1 to 18mV 42 In section 11 in the second row Weight changed stimulator to controller and changed 1 2 lbs to 1 1 Ibs Updated the cross references to the cautions on the inside front cover of the document December 2013 Added pattern generation and licensing and updated hardware Updated images screenshots and illustrations accordingly December 2012 This is the first release of the manual PlexBright 4 Channel Optogenetic Controller with Radiant IV Plexon Inc Radiant Version 2 0 Plexon Inc V Contents Publication History PlexBright 4 Channel Optogenetic Controller with Radiant Software Before You Begin 1 2 Introduction 1 3 System Requirements 1 4 System Components 1 5 Installation 1 7 Getting Started 1 14 GUI Function Reference 1 28 Input and Output Co
26. exon Radiant Version 2 Windows x86 Installs E x 4 Plexon Radiant Version 2 Windows x64 InstallShield Wizard C Installing Plexon Radiant Version 2 Windows x06 The program feghunes you seee hod ane Geng instabed Installing Plexon Radiant Version 2 Windows x64 The program features you selected are being installed Pease wail while Une Inetalshek Witerd istak Peon Radiant Version 2 Windows 386 This may take several minutes Please wait while the InstallShield Wizard installs Plexon Radiant Version 2 hear Windows x64 This may take several minutes Status Status InstallShield PlexBright 4 Channel Optogenetic Controller with Radiant 7 An Opal Kelly FrontPanel USB Driver Setup window will pop up Click Next amp Opal Kelly FrontPanel USB Driver Setup E Welcome to the Opal Kelly FrontPanel USB Driver Setup This wizard will quide you through the installation of the FrontPanel Driver only package We suggest you uninstall any previous versions of FrontPanel and make sure that FrontPanel is not currently running It is also recommended that you close all other applications before continuing This will make it possible to update relevant system files without having to reboot your computer Click Next to continue Cancel 8 Accept the Opal Kelly License Agreement 6 Opal Kelly FrontPanel USB Driver Setup E License Agreement a a pa K el ly Please review the license
27. g d Light 7 mW Calculates 210 mA Light Output mW c O w i 0 50 100 150 200 250 Current mA SS In order to find the light output at the max current the entire system should be assembled PlexBright 4 Channel Optogenetic Controller BNC cable LED module Optical patch cable Optical fiber implant The current is then manually set to the maximum output 300mA and the light output at the end of the implant is measured with a light meter This will compensate for any losses at each optical connection as well as any variability between LED modules A similar calibration can be performed for lasers except that the requested light output will be converted to a voltage If LASER is the output device selecting Power mW as the output mode will bring up Voltage Output Limit and Light Power fields You need to manually enter the maximum voltage usually 5000 and the corresponding power measured at full voltage If LED is the output device the maximum current is determined by the LED Type The user only needs to enter the measured light power at full current If the LED Type is set to Custom both Current Output Limit and measured Light Power at the maximum current need to be entered As a voltage example for lasers put 40mW as the power at 5V Next request 20mW of light in Manual mode and measure the actual voltage being output on an oscilloscop
28. here are two types of files opt and txt An opt file is created with the Pattern Generator and has a header that defines stimulation parameters such as units while a txt file is created with a text editor and is just a series of amplitudes 7 R Open s Me Ee Look in B Documents v ean ee E E Name Date modified Type ma 7 J Scanned Documents 12 4 2012 10 32 AM File folder Recent Places i 3 P National Instruments 7 12 2012 11 07 AM File folder A J MATLAB 6 26 20122 51PM_ File folder Desktop Ji Xilinx 10 10 2011 2 06 PM File folder Visual Studio 2005 9 29 2011 9 55AM File folder al dJ National Instruments 7 12 2011 5 22PM File folder Libraries di Texas Instruments 7 5 2011 3 42 PM File folder A d PlanAhead_Projects 3 7 2011 10 27 AM File folder gt di Fax 9 20 2010 1 16 PM File folder Computer Ji microsoft 12 11 2009 2 54 PM_ File folder N m J Files of type Pattem Files with headers opt X o Cancel I Open as read only Select the file pulse_bursts txt that is located at C Plexon Data Sample Pattern Files Note that the pattern files are Read Only To change them first copy and Radiant Version 2 0 21 22 rename the original and then remove the Read Only flag by right clicking the file name and selecting Properties from the drop down A dialog box will pop up explaining the units being applied to the file PlexBright 4h The file you opened doesn t have a header P Since th
29. herited fram channel opt File Units coded in File Set Repetitions 15 6 3 Launching the Radiant Software Make sure all of the PlexBright 4 Channel Optogenetic Controllers are connected to the computer and turned on Up to four controllers can be used with a single computer Then launch the Radiant software by double clicking on the RadiantV2 desktop icon You should see the factory default graphical user interface as shown in the figure below Note that the number of controllers that are connected will determine the number of tabs in the bottom left of the screen If multiple controllers are connected but their tabs do not show up they can be added by from the View drop down in the menu bar Also the LEDs on the end panel of the controller will indicate the device number 1 4 nah iisa e ee awe E mtt TIP Restoring factory settings You may load the factory default configuration at any time by clicking File Restore Factory Settings Note that if the USB cable is not connected to the controller or if the controller power is turned off when the software is started the software will open the pattern generator only as seen in the image below There will be no window for configuring stimulation channels ee r r ami oree meae PlexBright 4 Channel Optogenetic Controller with Radiant If you see the window like the one shown below and your stimulator is connected it is
30. ht 4 Channel Optogenetic Controller with Radiant Windows 7 Windows XP iD Found New Hardware Opal Kelly XEM6001 3 i Installing device driver software Click here for status a Gaat pe Piceri Welcome to the Found Naw H rg Wizard Thei mitad haipiti lor Dow Fey L HETI I pha hander came mih an maiallatice CO a fe opp dak memi d mr Waha do au aan Ha scd o dot E ratai he steir miorre iy Herrera O rmi her a bed r presota katon chert Check Hoi be panirat Found Mew Hardware Wizard Found New Hardware Wizard Compl ting th Found Hew Hardware Wizard The soa ha rehej raian ther iaa ior gt Goa sk MET Dih Fire io gkg the mgd ea i Found New Hardware x 71 Opal Kelly XEM6001 ax Device driver software installed successfully Contact a PlexBrig Your new hardware is installed and ready to use The first time that you plug in the USB license key you will see an installation message as well SafeNet USB SuperPro UltraPro AG Device driver software installed successfully Radiant Version 2 0 13 6 Getting Started 14 This section introduces the basic functions of the PlexBright 4 Channel Optogenetic Controller and guides you through basic operation using the voltage mode Voltage mode is intended to be used to control lasers with a 0 5 V control input The current mode functions similarly for controlling LEDs but it is more difficult to quantify the outp
31. is updated every 100us A user defined pattern file is simply an ASCII text file that contains a list of stimulation output values that are desired every 100us Each value is followed by a line feed and a carriage return This means that if you want to maintain the same value for a longer period of time you must repeat the value desired time 100us times For example stimulating 100mA for lms would require 10 consecutive lines of 100 Pattern Generator The Pattern Generator is a tool for creating patterns consisting of square pulses ramp pulses and constant outputs that can be grouped and repeated The hierarchy used in the Pattern Generator is Pattern Group Primitive Pattern A pattern consists of one or more groups and can be repeated It has units associated with it that must match the units of any channel that it 1s loaded to Group A group consists of one or more primitives and can be repeated It has a period at which it is repeated As more primitives are added the period automatically lengthens to accommodate the added primitives If primitives are deleted the period will not automatically shorten Primitive There are three types of primitives constant value square pulse and ramp pulse A constant value holds an output for a set amount of time A square pulse holds a Radiant Version 2 0 29 value for its width and then returns to zero output for the remainder of its period A ramp increases f
32. king 2 5 mm inner 5 5 mm outer barrel connector The stimulator operates from a grounded 12V power supply Pin Funetion Power in 8 2 Digital In Each channel in the PlexBright 4 Channel Optogenetic Controller has two digital inputs associated with it one called Start and one called Stop In Edge Digital Input mode the channel can be started with the start input and stopped with the Stop input In Level Digital Input mode the Start input can be used to both start and stop the channel See Section 6 5 Starting and Stopping Stimulation on page 18 and Section 7 7 Trigger Options on page 30 Digital Input Triggering for additional details The inputs are TTL compatible 13 Start 4 NC 11 NC T ge oO 0O I ie a E 15 NC 9 Start3 5 Start 2 1 Start1 NJ e e kr e co OM D N T co O3 D N O 1 1 1 i 1 1 1 I I Z Fa zZ Ww 0 Mf zZz 9 2Y A GF opo 3 3 O O O Q Q Q Q GB GB N pi ts UJ Radiant Version 2 0 33 34 8 3 USB 2 0 The stimulator has a type B USB 2 0 receptacle for communications with the host computer The device is not powered from the USB bus It derives power from the power input connector 8 4 Current or Voltage Output Connectors The stimulation output consists of four BNC connectors one for each channel In voltage mode the inner contact is the output voltage and the outer contact is connected to ground In current mode the current going to the LED leaves th
33. nnectors 1 33 Sample Arbitrary Waveform Pattern Files 1 37 Optogenetic Controller Limitations 1 38 Specifications 1 41 PlexBright 4 Channel Optogenetic Controller with Radiant vi Plexon Inc Radiant Version 2 0 PlexBright 4 Channel Optogenetic Controller with Radiant Software Radiant Version 2 0 1 Before You Begin Before using your system please check www plexon com downloads html for any software updates PlexBright 4 Channel Optogenetic Controllers manufactured prior to November 21 2013 are eligible for a no charge upgrade to revision A You can determine the revision of the controller by looking at the label on the bottom of the controller or by looking in the Radiant software under the info section for each controller Radiant Version 2 0 and later Please see the caution on the inside front cover Contact Plexon support at 1 214 369 4957 or support plexon com for additional information The information in this user guide refers to e Hardware PN 08 06 A 04 08 06 A 04 A 08 06 A 04 B and 08 06 A 04 C e Firmware PN 08 06 A 32 e Radiant software Version 2 0 2 PlexBright 4 Channel Optogenetic Controller with Radiant 2 Introduction The PlexBright 4 Channel Optogenetic Controller is a 4 channel current output LED mode or voltage output Laser mode device It has 4 individually programmable channels that can be configured from a host computer using the Radiant graphical user interface software or config
34. ount Repetition 1 Repetition gt Repetitions 3 Tae E Primitive 2 Constant Pattern Value ma 0 Duration ms 50 E Primitive 3 Ramp Value mA 25 Period Freq Period Period ms 50 Ramp Width ms 25 Total Duration Repe Repetitions Count Repetitions 2 PlexBright 4 Channel Optogenetic Controller with Radiant After a pattern has been defined it needs to be associated with a channel The units of the pattern must match the units of the channel Select Pattern Generator from the Pattern Source drop down Then select the pattern that you want to load GD az 3 m 4 jA DI Mode Edge DI Polarity Positive Stimulation Status Ready Output Device LED LED Type Blue 465 nm 300 mA fixed Output Mode Current mA Pattern Source Pattern Generator Patterns Pattern 1 Reload Pattern Reload If the units of the pattern do not match the units of the channel the following error message will pop up Radiant The pattern can t be selected for this channel since it has different measurement units Change units or try to apply a different pattern to this channel Radiant Version 2 0 27 7 GUI Function Reference 28 7 1 Status The status of a given channel is either Not Ready or Ready Until a pattern for a channel has been defined the status bar will say Not Ready and the start button for that channel will be grayed out Digital inputs to that ch
35. roller that is connected to the computer will have an additional window for Stimulator and Patterns The number corresponds to the LED number that is illuminated under Device on the end panel of the controller Selecting a window under the View menu will cause it be highlighted in the GUI the title bar turns orange if it is present in the current layout If the selected window is not present in the current layout selecting it will cause it to be displayed 7 10 Window Like most modern software Radiant allows the user to make considerable customizations to the appearance of the graphical user interface Each window Radiant Version 2 0 31 32 can be resized docked undocked or hidden completely Once you have configured the GUI the way you want it you can save the layout by selecting Window Layout Save As Layout 1 Save As Layout 2 or Save Layout to file Saved layouts can be reloaded by selecting Apply Layout 1 Apply Layout 2 or Load Layout From File You can also return to the default layout at any time by selecting Reset to Default Layout 7 11 Help The Help menu gives access to the User Manual as well as the software version number PlexBright 4 Channel Optogenetic Controller with Radiant 8 Input and Output Connectors 8 1 Power In This information is provided for reference only Use the recommended power supply and attached cable provided by Plexon to power the controller The power input connector is a loc
36. rom touching This is particularly useful when using the controller in current output mode The circuit that sets the current output relies on measuring the return current so 1f the outer BNC contact were shorted to ground through accidental contact with something else a metal table for example the output current would not be accurate PlexBright 4 Channel Optogenetic Controller with Radiant 9 Sample Arbitrary Waveform Pattern Files Several example arbitrary waveform files are installed with the Radiant software These files were designed to illustrate how to use arbitrary waveform files and some of the things you can accomplish using arbitrary waveform files By default these files are installed in the directory C Plexon Data Sample Pattern Files The files can be opened with any text editor e g Notepad The sample files are marked as Read Only They should be copied and renamed before removing the Read Only flag to avoid accidentally overwriting them Radiant Version 2 0 37 10 Optogenetic Controller Limitations 38 10 1 Latency between Digital Input and Voltage Output The timing dynamics in voltage mode of starting the first channel with a digital input are shown below All digital outputs change to reflect that a digital input trigger has been detected within 200ns though not exactly at the same time The voltage output begins to change 9 5us after the digital input trigger and the rise time of the voltage output is less
37. rom zero to its value over the pulse width It then returns to zero output for the remainder of the period 7 6 Repetitions and Count File Mode A file based pattern can be played continuously or a finite number of times based on the setting of the Repetitions parameter When Finite is selected for Repetitions the number of repetitions may be set using the Count parameter Note that these controls are not visible when Manual Mode or Pattern Generator is selected as the pattern source 7 7 Trigger Options There are three ways to initiate stimulation Software Edge Digital Input and Level Digital Input In all cases the stimulation pattern for the channel must be defined before it can be triggered and the channel status must be ready Software Triggering Stimulation can be initiated by clicking on the Start or Start All buttons in the GUI or calling one of the start functions in the SDK Each time Start or Start All is pressed the stimulation pattern for the channel is initiated Digital Input Triggering Edge DI Mode When the DI mode is set to Edge stimulation begins when the Start digital input for the channel transitions from low OV to high 5V if DI Polarity is set to Positive If DI polarity is set to Negative stimulation begins when the digital input transitions from high to low The latency from digital input to stimulation onset is less than 10 us for the first channel that is started Since all channels are timed from
38. sitive to negative stimulation will start Negative DI Polarity If DI Polarity is set to Negative and the device is in Edge triggered mode the controller responds to falling edges on the Start and Stop digital input pins In Level triggered mode the controller starts on a falling edge and stops on a rising edge of the Start digital input Note that changing polarity while a channel is loaded may cause it to start depending on the state of the digital input If a digital input is at 5V and you polarity is changed from negative to positive stimulation will start 7 8 File Load Settings File Save Settings The settings for all channels can be saved to a user named file for future use by clicking the Save icon or by selecting Save from the File menu These configuration files have an extension of ops The ops file saves the configuration of each channel and all of the patterns defined in the pattern generator It does not save the window layout see Section 7 10 Window on page 31 The default values of the parameters can be restored by selecting File Restore Factory Settings You can also go to Window Layout Reset to Default Layout to set the windows of the GUI back to their default state 7 9 View When one controller is connected to the computer there will be five choices under the View menu bar Stimulator 1 1 Patterns 1 Pattern Generator Pattern Generator Visualizations and Messages Each additional cont
39. t Each channel has four dedicated digital outputs that indicate when it is running non zero zero and when it stops Plexon OmniPlex systems both analog and digital have digital input DI cards that allow the user to register the precise timing of stimulation The DI cards can only be set to recognize rising or falling edges however This means that to fully know the timing of a pattern multiple digital outputs of the PlexBright 4 Channel Optogenetic Controller need to be monitored The rising edge of the running signal indicates a pattern has started and the rising edge on the stop signal indicates the pattern has finished The rising edge of the non zero signal indicates that a pulse has started and the next rising edge of the zero signal indicates that the pulse has ended Note that if there are programmed zeros at the end of the pattern the running output will stay high until the zeros are done playing at which point the stop signal will go high Current Voltage Output Running f Non zera ee stop a 1119 Gnd 17 Gnd 13 Running 4 11 Stop 3 9 Running 3 wae Stop 5 Running 2 3 Stop 1 1 Running 1 I a oO i wy wi i l 3 quaz 1 roaz OT zuon pti E saz ZT Z UON OT azu 96 oft Touez pl y az UON Z 8 7 Stimulation Cables While any BNC cable can be used for stimulation Plexon provides insulated BNC cables that prevent the outer contacts f
40. the same 10 kHz clock there is a 100 us variability when starting subsequent channels This is because they will not start until the next 10 kHz clock edge comes following the digital input Stopping stimulation also has a 100 us variability because the pulse cannot end until a 1OkHz clock edge is seen Once the stimulation pattern is triggered it will play to completion even if the Start digital input goes low during the stimulation The pattern can be stopped by a rising edge on the Stop digital input Level DI Mode When the DI mode is set to Level stimulation also begins when the Start digital input for the channel transitions from low OV to high SV when DI Polarity is set to Positive and continues until the Start digital input goes low again The reverse is true if DI Polarity is set to Negative The Stop digital input is not used in Level triggered mode PlexBright 4 Channel Optogenetic Controller with Radiant Positive DI Polarity If DI Polarity is set to Positive and the device is in Edge triggered mode the controller responds to rising edges on the digital input start and stop pins In Level triggered mode the controller starts on a rising edge of the Start digital input and stops on a falling edge of the Start digital input Note that changing polarity while a channel is loaded may cause it to start immediately depending on the current state of the digital input If a digital input is at OV and the polarity is changed from po
41. u must manually enter the current limit for the custom LED Reducing the current limit also has the additional benefit of increasing output resolution because the output always has 8 bit resolution between zero output and the current limit So if your limit is 1000mA your resolution will be approximately 3 9mA Reducing the limit to 300mA makes your resolution 1 2mA In voltage mode the output can range from O 5V If you do not need the full SV range you can increase the output resolution by setting a voltage limit that is below SV PlexBright 4 Channel Optogenetic Controller with Radiant 7 5 Pattern Source There are three options within the Pattern drop down Manual Mode File and Pattern Generator Manual Mode In manual mode a value is entered for the amplitude of a constant output The units are mV mA or mW If power mW is used to specify the output a current to power or voltage to power scaling must be performed as outlined in section 6 9 File In file mode the output amplitude of the channel is controlled by amplitude values read from a txt or opt file The txt file is just a list of numbers see below When the txt file is opened the output device and output mode of the channel determine how the file is interpreted The opt file contains header information that specifies the output device and output mode Creating a stimulation pattern file The controller operates at 1OkHz meaning the stimulation output
42. ured from user written applications by means of a software development kit SDK In LED mode the controller can output up to 1100mA from each channel and the compliance voltage can range up to 12V to drive multiple LEDs in series In Laser mode the controller can output voltages from 0 5V for modulating the output of a Laser or other device User defined output limits may be defined with 8 bit precision and output waveforms may be defined with 8 bit resolution within those output limits Playback of pulses and arbitrary stimulation waveforms may be initiated from the host PC or triggered in response to external digital inputs Each channel has a dedicated start and stop digital input that may be used to initiate and halt stimulation Each channel also has four dedicated digital outputs to signal to other devices the precise time when stimulation events are occurring The Radiant 2 0 graphical user interface incorporates a new Pattern Generator tool that allows you to quickly define complex output patterns for the PlexBright 4 Channel Optogenetic Controller to play back Arbitrary stimulation patterns can also be loaded from user defined text files or the outputs can be controlled directly by the user in manual mode Thank you for purchasing this Plexon product We hope you are pleased with every aspect of it Please do not hesitate to contact us if you have any questions Radiant Version 2 0 3 3 System Requirements A modern personal comp
43. ut without a light meter It is highly recommended that you work through this section before attempting to do any type of stimulation Note that you will need an oscilloscope to see the output 6 1 Overview Every optogenetic stimulation protocol begins with the definition of the stimulation pattern The graphical user interface GUI provides a means for manually setting the output value creating a stimulation with the pattern generator or loading a stimulation pattern from a user created text file Once defined the stimulation pattern is downloaded into the controller memory for playback Playback can be initiated from the GUI SDK or in response to a digital input to the controller hardware PlexBright 4 Channel Optogenetic Controller with Radiant 6 2 Operational Flowchart Start Radiant Software Set Output Device LED Mode Set LED Type Set Output Mode Curent or Power Set Output Limit furent or Power Set Pattern Source Set Pattern Source Manual Mode Pattern Generator Entered Desired Create Pattern s in Qutput Generator Curent Voltage or Power Set Pattern Units LED mA LED mi LASER mv LASER mha Add Groups and Primitives Assign Pattern to Channel Start Stimulation Radiant Version 2 0 Set Output Device LASER Mode Set Output Mode Volpe or Power Set Output Limit Wohage or Power Set Pattern ource File Browse to File xt File Unitsin
44. uter running Windows 7 or Windows XP with a free USB 2 0 port and 1 GB of memory is required to operate the system 4 PlexBright 4 Channel Optogenetic Controller with Radiant 4 System Components When you receive your PlexBright 4 Channel Optogenetic Controller confirm that you have the following pieces 1 USB memory with software and drivers in box Plexon 2 AC power cord 7 5 ft Volex 17250 10 B1 3 Power Supply Plexon 08 06 A 37 4 USB Cable 2m Monoprice 5438 3 Optogenetic Controller Plexon 08 06 A 04 6 License Key in software box Plexon 7 Color coded Insulated BNC Cables 4 Plexon 06 03 A 04 CCLL International customers The stimulator power supply has an International Electrotechnical Commission IEC 60320 C14 inlet for AC power shown below The AC power cord supplied with the stimulator has an IEC 60320 C13 connector and a NEMA 5 15 plug compatible with the AC wall outlets in North America Users in other regions must supply a power cord with an IEC 60320 C13 connector and a plug that is compatible with the AC wall outlets in the region of use The hardware and cables are shown in the following photograph Radiant Version 2 0 Optogenetic Controller SPLexon rer PlexBright 4 Channel Optogenetic Controller with Radiant 5 Installation Read the entire installation section before proceeding with the installation Follow the installation steps in the order that they are presented Install the softwar
45. xt File Stimulation patterns can be defined in simple text files using any text editor software Microsoft Word Notepad etc More advanced users can programmatically create files using software such as Matlab or Labview The text file is simply a list of amplitudes When it is played back each amplitude is updated every 100us To maintain a value longer than 100us the value is repeated Several sample files are included in the installation package The amplitude units can be mA mV or mW The user specifies the units when setting up the channel before loading the txt file PlexBright 4 Channel Optogenetic Controller with Radiant 6 7 Loading a Pattern from a txt File First set the Output device as LASER output mode as Voltage mV and Voltage Output Limit mV to 5000 Begin by selecting File from the Pattern Source drop down menu Channel 1 Stimulation Status Re EA Output Device LASER Qutput Mode Voltage mV Voltage Output Limit mV 5000 Pattern Source Manual Mode Voltage Output mV Manual Mode a Channel 2 File Stimulation Status Pattern Generator Output Device LED La Next click on the folder in the field next to Load from File Channel 1 Stimulation Status wit Output Device LASER i Output Mode Voltage mV Voltage Output Limit mV 5000 Pattern Source File x O Loadfrom File j E i Repetitions Finite Count i A dialog box will pop up T
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