YSI 6600 Sonde manual
Contents
1. pH mV buffer 7 0 MV 50 pH mV buffer 10 177 from pH 7 mV span b w pH4 7 mV 165 to 180 mV span b w pH7 10 mV Maintenance Record 165 to 180 mV Sensor Completed Y N N A Batteries changed If not battery voltage 28 Logging data setting up the calibrated sonde to be deployed This instrument can log store data in two locations 1 YSI 650 display unit 2 The sonde Option 2 logging to the sonde is the option used for long term deployments i e the sonde is not attached to the display unit and is put into a river or lake to collect several weeks worth of data For these NWT ENR sondes we will be logging data continuously at a Set time interval every 15 minutes to the memory within the sonde To set up logging and deploy the sonde Step 1 Make sure that the sonde is not being powered by the 650 650 main menu system setup the box for power sonde from 650 should NOT be checked The sonde must be powered by its own batteries Step 2 From the sonde Main menu select Run Step 3 Select Unattended Step 4 On the Unattended setup menu the settings should read as follows Interval 00 15 00 Start date enter the date of deployment in dd mm yy Start time enter the approximate time of deployment as the start time Duration days number of days of deployment note must be less than battery life below File Enter a name for the file here Site Enter the site n2. Notice the four small holes on the lid Barometric Pressure is read through these four holes Never cover them with tape or stickers or push anything through these holes e g pins Step 2 Insert the batteries between the clips being sure that the orientation is correct A foam pad on the back of the lid will help to hold the batteries in place when the lid is put back on m e j Cs ee Step 3 Re install the battery lid and tighten the screws Do not overtighten Connecting the Sonde to the YSI 650 Display Unit It is extremely important to keep water out of the electrical connections between the display field cable and sonde Tip connect the instruments in dry environments only e g inside your vehicle at the field site if it is raining not outdoors To connect the sonde to the YSI 650 display unit you will need 1 the sonde 2 the 650 MDS display unit with batteries installed 3 the field cable F e 650 display unit Field cable Sonde Step 1 remove the waterproof cap from the sonde connector Do not discard this cap You will need this cap for later deployment and long term storage Step 2 connect the field cable to the sonde connector There is a guide inside the connection that ensures that the pins are properly lined up Rotate the cable gently until the guide engages Step 3 Tighten the connector by turning clockwise When connected properly only two threads should
3. 5 10 minutes until the air in the cup saturates with water After allowing the dissolved oxygen reading to stabilize record the value in mg L and on the calibration sheet under after deployment reading Step 6 Remove the sonde from the calibration cup and discard the water 33 Step 7 Dry the calibration cup with lint free cloth i e Kim Wipe 2 Conductivity and Temperature Step 1 Screw the calibration cup onto the YSI and invert it sensors pointing to ceiling Step 2 Fill the calibration cup with specific conductivity standard 1413 uS cm so that the conductivity sensor and the temperature sensor are submersed into the calibration solution Step 3 Record the value of conductivity in uS cm under after deployment reading Step 4 Remove the calibration cup cap and add a thermometer to the solution and allow reading to stabilize Step 5 Record thermometer reading on worksheet under temperature standard 3 pH Step 1 Rinse the pH sensor only once with small amount of pH 7 solution Step 2 With the sonde inverted fill the calibration cup with pH 7 solution to immerse the pH sensor and the temperature sensor on the conductivity probe Step 3 Record the pH reading on the calibration sheet under after deployment reading Step 4 Rinse the sensors and cup with a small amount of water 4 Oxidation Reduction Potential Step 1 Rinse the pH ORP sensor with a small amount of Zobell s solution St
4. be visible Step 4 Attach the strain relief connector to the metal handle and close the connector s opening Sas BEIDE Si FIELD CABLE E CONNECTOR I REMOVE WATERPROOF CAP y SONDE T CONNECTOR p aol STRAIN RELIEF CONNECTOR ar K poe Il y i A HANDLE Step 5 At its other end the field cable plugs directly into the 650 display module using an 8 pin connector Line up the guides and pins inside the connector Be careful not to cross thread Gently turn the nut clockwise until it clicks to lock the connector in place Attach the second strain relief connector to the display unit 12 Initial Setup Your particular community s sonde and 650 display unit have settings that are identical to those in all the other sondes and display units purchased by ENR for the other communities in the NWT If all the communities work with the same instruments settings and operating instructions the water quality data generated at the different sites will be consistent and comparable This will help to evaluate all the water quality data once it is collected It is important for you to be able to access and understand these settings but under normal operation they should not need to be changed Here is a description of how to access the settings Below is a photo of the 650 display unit with the different buttons identified 650 Front View Display Backlight Key On Off Key Arrow Keys Enter Key Escape K
5. data setting up the calibrated sonde to be deployed seeeeeeeees 29 After a Sonde Deployment Retrieving the sonde downloading the data ANG CHECKING TOR GNM xcurseete sets ccc pent ties ea trace cael eraa alee t sities 30 SONGS TEMIEV all ertreeclicelindsiet E elicitin ist iciion 30 Downloading Water Quality Data from the Sonde to a Computel eeeeeee 30 After deployment check for drift ccccceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeaaeeeeeeeeeeeeeeeenaaees 32 Supplies needed to check for drift cccccceeeceeeeeeeeeeeeeeeeeeeeeeeeeeeeeaeeeeeeeeeeeeeeeees 33 Instructions Checking for drift ccccceeceeeeeseeeeeeeeeeeeeeeeeeeeeeaeeeeeeeeeeeeeseeeneeaeeeeeeees 33 1 T rbidity a d DO sei aaaea enana ataa eaa aa aaa a aa Ea aA Ea Aa Eaa aAa Da Eaa 33 2 Conductivity and T SINPCKAlUlS ius ence covet rceensaenes cmweteverwcsivecancsiencieceveciansaemcsenestee 34 EN D a LEA A ae rae ae A eT DO aA ETN ODE OORT eR TO rae rer een REa E Pence at rte eer 34 4 Oxidation Reduction Potential ccccccceeeeeeeeceeeeeeeeeeeeeeeeeneeeeeeeeeeeeeeeeeneees 34 15 Om B 9 Lo ener eeprom a Te Ten mee ern ee eee ere eer 34 Cleaning the Sonde areca esate dec rhe ete oe cetera Sle dda ie hited sect eaten 35 Re Calibration and Re deployment cccceeeeeeeeeeeeeeeeeeeeeeeeeeeeseeenaaeeeeeeeeeeeeeneas 35 SONSO IGA ce fa al hee AE AE ee a A eet eho cache Meee Mia aA
6. in the plug tops and gently unscrewing The installation tool can be found in the maintenance kit YSI 6570 Always remove plugs and sensors from the sonde with that end of the sonde pointed towards the ground or floor i e oriented as in the picture below f there is water present on the sensors or plugs removing them as they are pointed down will allow water to drain away and prevent any flow of water up into the electrical connections inside the ports Installation tool Sonde bulkhead Port plug Step 2 Install Sensors This sonde has two different types of ports capable of accepting sensors Optical ports There are four optical ports on this sonde labelled as O B C and T O port is for the optical Dissolved Oxygen sensor model YSI 6150 C port is for the optical Chlorophyll sensor model YSI 6136 T port is for the optical Turbidity sensor model YSI 6025 B port currently has no optical sensor so it is Blank for now Regular ports There are two regular ports They are not labeled but they are different 1 The 6 pin port is for the Temperature Conductivity sensor model YSI 6560 2 The 4 pin port is for the pH Oxidation Reduction Potential sensor model YSI 6569 6 pin Temperature and Conductivity Chlorophyll Optical Turbidity Optical Blank Optical Dissolved Oxygen Optical 4 pin pH and Oxidation Reduction Potential ORP Before inserting any sensor ensure that the port is fre
7. not vary from the historical value more than 0 1 look back at previous calibration sheets to determine the historical value If the value doesn t meet these criteria the calibration standard may be off Try another bottle of calibration standard If the constant is still off DO NOT DEPLOY this sensor and contact ENR for support Step 13 Turn the sonde back to normal orientation Remove the calibration cup and add a thermometer to the solution and allow the reading to stabilize Record the temperature value from the thermometer in C in the box for temperature standard on the calibration worksheet Step 14 Empty the calibration cup Rinse all the sensors and the calibration cup thoroughly with de ionized water before calibrating the next parameter 22 4 pH Note pH requires a 2 point calibration Note Before pH calibration make sure pH in millivolts is turned on under Sonde Menu Report Step 1 Rinse the sensors and calibration cup once with de ionized water Step 2 Rinse the sensors and calibration cup with pH 7 solution repeat this step two more times for a total of 3 rinses with pH 7 solution Step 3 With the sonde inverted fill the calibration cup with pH 7 solution to immerse the pH bulb and the temperature sensor on the conductivity probe Step 4 From the Sonde menu select Calibrate press enter Step 5 Select ISE1 pH press enter Step 6 Select 2 Point press enter Step 7 Input the v
8. sensors should be pointing to ceiling Step 4 Fill the calibration cup with conductivity standard 1413 uS cm 1 413 mS cm at 25 C so that the conductivity probe and the temperature sensor are completely submersed into the calibration solution i e past the vent hole Rotate the sonde to ensure that there are no air bubbles inside any part of the conductivity sensor Wait a minute before moving to the next step to allow the temperature to stabilize Step 5 From the Sonde menu select Calibrate and press enter Step 6 Select Conductivity and press enter Step 7 Select SpCond specific conductance and press enter Step 8 Use the keypad to enter the known concentration of the conductivity standard in mS cm at 25 C For example 1 413 mS cm Press Enter to confirm your input The values of all the sensors will appear on the screen and will change as the readings stabilize Step 9 Record the pre calibration value of conductivity in uS cm Step 10 Highlight CALIBRATE Once the temperature reading is stable and the conductivity reading is stable press Enter Step 11 Record the post calibration conductivity value in pS cm on the calibration worksheet Record the temperature value in C from the sonde on the calibration worksheet Step 12 Record the conductivity cal constant on the calibration worksheet You can find the conductivity cal constant under Sonde Menu Advanced Cal Constants It should fall between 4 55 5 45 and
9. to the sonde and basically talking to it In this state the small picture in the bottom right hand corner of the 650 display is a picture of the sonde In this communication state you can calibrate the sonde and change sonde settings _ Main a l Calibrate File Status System Report Sensor Advanced N 0 0 000 E 0 0 000 740 4mmig 05 31 20001421 14 Hmmm Sonde Icon Emm 2 In the second state the display unit is not connected electronically to the sonde and is not talking to it In this case the icon in the bottom right hand corner of the 650 display will be the 650 display itself 650 Main Menu Sonde run Sonde menu File Logging setup System setup 650 display WN 0 0 000 E 0 0 000 742 0mmig z unit icon 06 06 2000 17 40 26 F TH For the next few sections we are just setting up the basic display unit itself so we do not need to be connected to the sonde When you are working with the instrument make sure it is in the communication state you desire by checking the icon in the corner 15 Main Menu After physically connecting the sonde to the display unit as described in the previous sections turn on the YSI 650 display unit using the green power button Notice that in this picture you can see that we are not talking to the sonde You will see the following Main Menu 650 Main Menu Sonde run Sonde menu File Logging setup System setup N 0 0 0
10. turbidity sensor model YSI 6136 Therefore the second turbidity standard value that you will need to input is 126 NTU ORP standard Zobell s solution 500 mL Calibration worksheet located at the end of this section Sonde maintenance kit Protective gloves wear these during all calibration steps Calibration Instructions 1 Dissolved Oxygen Note Before dissolved oxygen calibration make sure ODO sat L and ODO mg L are turned on under Sonde Menu Report Step 1 Add a few mL of de ionized water to the calibration cup Step 2 Screw the calibration cup onto the sonde only engaging a few threads so air can diffuse into calibration cup Do not tighten the cup completely onto the sonde Be sure there are no water droplets on the dissolved oxygen sensor or on the temperature sensor Ensure these two sensors are not submerged in water Step 3 From the Sonde menu select Calibrate press enter Step 4 Select Optic O Dissolved Oxy press enter Step 5 Select ODOsat press enter Step 6 Select 1 point press enter Step 7 Enter the barometric pressure reading at the bottom right corner of the screen and press enter Record the barometric pressure reading on the calibration sheet 20 Step 8 After allowing the dissolved oxygen reading to stabilize for 5 15 minutes record the pre calibration value in mg L and in L local on the calibration worksheet Step 9 Highlight CALIBRATE and press enter to calibrate t
11. will display readings Step 16 Activate the sensor wiper 1 2 times by pressing 3 Clean Optics Step 17 When the readings have stabilized record the pre calibration turbidity reading for 126 NTU on the calibration worksheet Step 18 Press enter to confirm the second calibration and record the post calibration value for 126 NTU on the calibration worksheet Step 19 Empty the cup and rinse with DI water 27 Calibration Sheet Date Time Sonde Checked by Location Time since last calibration Parameter Standard Used Before Calibration Reading After Deployment Reading if applicable After Calibration Reading Barometric Pressure mmHg sonde Temperature C sonde Temperature C thermometer reading thermometer Specific Conductance uS cm 0 1413 Dissolved Oxygen L local mg L pH 4 7 10 Calibrate Turbidity NTU 0 Clean Sonde 123 Oxidation Reduction Potential ORP Zobells Chlorophyll DI water Depth m 0 in air 1m in water Comments YSI DETAILS VERY IMPORTANT TO FILL THIS CHART IN Parameter conductivity cell constant acceptable range 5 0 0 45 amp not more than 0 1 from historical record ODO gain value should be between 0 75 1 25 pressure offset 14 7 6 non vented
12. 00 E 0 0 000 742 0mmng C 06 06 2000 17 40 26 a E E System setup Date and Time To set or change the date and time in the YSI 650 display unit Step 1 Select System setup from the main menu System setup Date amp time Date amp time LlPower sonde Baud rate to sonde 9600 Shut off time minutes 0 JComma radix ID Barometer units N 0 0 000 E 0 0 000 02 29 2000 15 21 05 Step 2 Select Date amp time Date amp time setup m d y Od m y Oy m d M4 digit year Date 5 31 2000 Time 11 55 24 N 0 0 000 E 0 0 000 05 31 2000 11 55 30 Step 3 Select d m y as the date display format Step 4 Ensure there is a check mark beside 4 digit year Step 5 Enter the current date in the format d m y Step 6 Enter the current time Note that it is critical that you keep track of the time zone the data is collected in and whether or not the time of the instrument was changed when the clocks are put forward and back for local daylight savings Without good field records it is very difficult to determine when data has been collected Barometer Calibration There is an option under System setup called Calibrate Barometer The barometer in the 650 display unit should only ever be calibrated using local barometric pressure i e pressure that has not been corrected for sea level Local weather service barometric pressure is often corrected to sea level and is therefore not the same as the true loc
13. 35 Quality CORIO sesa e a r a E O OE 35 PRCICTONCOS n e a a E A I ees 35 Please see the video associated with this manual for visual demonstrations of the procedures presented in this manual The video times associated with the different manual sections are as follows 0 03 An Introduction to the YSI Sonde pg 4 0 24 Installing batteries in the 650 Display Unit pg 11 2 38 Installing batteries in the Sonde pg 6 5 34 Installing sensors and removing plugs pg 7 6 50 The Bulkhead pg 8 8 13 Installing Optical Sensors pg 9 10 07 Installing Regular Sensors pg 9 13 30 Installing Probe Guard and Calibration Cup pg 10 14 52 Connecting the Sonde to the 650 MDS Display Unit pg 11 17 23 Calibrating Dissolved Oxygen pg 20 22 34 Calibrating Depth pg 21 24 16 Calibrating Conductivity and Temperature pg 21 33 32 Calibrating pH pg 23 44 54 Calibrating ORP pg 24 47 51 Calibrating Chlorophyll pg 25 49 47 Calibrating Turbidity pg 26 YSI 6600 Sonde Introduction What is a Sonde A sonde is a piece of equipment used to l monitor water quality It is basically a PVC E pipe with a circuit board inside see picture to the right Properly calibrated sensors that measure different water quality parameters e g pH temperature dissolved oxygen are attached to one end of the sonde The sonde is placed in a lake or river and it collects data from the water at regular intervals When the sonde is removed from the wate
14. 4 buffer solution is fine for long term storage of all the other probes as well Quality Control Collect triplicate grab samples for lab analysis at the beginning middle and end of every field season The lab analyses will provide a comparison for the data being collected by the sonde Every time a sonde is collected downloaded calibrated and re deployed take one water sample for lab analysis References These instructions have been assembled and reproduced with modifications from YSI incorporated 6 Series Mutiparameter Water Quality Sondes User manual YSI incorporated 6 Series Sonde Quick Start Guide YSI incorporated Calibration Maintenance amp Troubleshooting Tips For YSI 6 Series Sondes amp Sensors 35 YSI incorporated YSI 650 MDS Multiparameter Display System Operations Manual Environment Canada Yellowknife YSI Calibration Instructions no drift and YSI drift and calibration instructions V2 Please see the video associated with this manual for visual demonstrations of the procedures presented in this manual The video times associated with the different manual sections are as follows 0 03 An Introduction to the YSI Sonde pg 4 0 24 Installing batteries in the 650 Display Unit pg 11 2 38 Installing batteries in the Sonde pg 6 5 34 Installing sensors and removing plugs pg 7 6 50 The Bulkhead pg 8 8 13 Installing Optical Sensors pg 9 10 07 Installing Regular Sensors pg 9 13 30 Installing Pr
15. YSI 6600 Sonde Instruction Manual Version 1 Contents MUA DOO Sonde aia itecs ged e Shae adieu cece ete a aaa Saad a dacorenees 4 Introduction What is a Sonde ccse c crete toe caters st ie lta tees Stat ats ei eh edt tad 4 What parameters does the sonde measure ceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeteeeneeaaees 4 Important Points to Remember s22 occ he aaa cause ane nena 5 VPN AL SS RAP Se secede etc ca tate Sinata cts teed ads wace cade Cactus cas E ET 6 Installing Batteries in the Sonde c cccceeeeeeeeeeeeeceeeeeeeeeeeeeeaaeaeeeeeeeeeeenensaaeees 6 Installing Sensors vosit a feot a teeta es ota eee apes sd ae hee 7 Optical Sensors aad aaaea aaa dE E aA A EE EAEE a 9 Reg lar SONS ONS rc secant esicarse ed tere eaan iaa ae ia nd ia SE 9 Installing Probe Guard or Calibration Cup eccceeeeeeeeeeeeeeeeeeeeeneeeeeeeeeeeeeeees 10 VSI G50 MDS Display Unit descexacsteesceetcn nace tesis escent eco ceeeeenaen duchess 11 Installing Batteries in the 650 Display Unit cece eccceceeeeee eee eeeeeeeeeeeeeeeeeeeeeeeees 11 Connecting the Sonde to the YSI 650 Display Unit 2 0 0 0 ceeeeeeeeeeeeeeeeeeeeeeeeees 11 Tut E VSS T 8 Zap rte were Peer a rte ney penny enn EP ieee em Untne ey UaEHe renee e tnentTt 13 Communication between the 650 display unit and the sonde cceeeeeeeeee 15 Man MenU ca cecil te ce i tc ei i AE AE AEA EEEN 16 System SetuP A war Ae a Oa aa a Aaa a SRA aha A Na ate oT
16. al barometric pressure value required by this instrument New instruments are programmed to measure true local pressure and should remain stable for a long time before requiring re calibration If you do need to calibrate the barometer Step 1 Determine the local uncorrected barometric pressure If there is no true local laboratory barometer reading available a weather service reading in mm of Hg that has been corrected to sea level can be uncorrected according to the following formula True BP sea level corrected BP 2 5 x local altitude in ft above sea level 100 Step 2 From the main menu select System Setup then Calibrate Barometer Step 3 Input the local barometric pressure using the keypad Step 4 Press the enter key 17 The Power Sonde Option Under Main Menu and System Setup there is an option called Power sonde that can be clicked on checkmark or off no checkmark System setup Date amp time Power sonde a i Baud rate to sonde 9600 P Shut off time minutes 0 JComma radix ID Barometer units N 0 0 000 E 0 0 000 02 29 2000 15 21 05 When Power sonde is clicked ON the display unit powers the sonde When Power sonde is clicked OFF the display unit does not power the sonde and the sonde relies on its own batteries for power It is important that the Power sonde option be OFF when the sonde is going to be deployed Otherwise the displayed battery life information i e how long t
17. alue of the pH 7 solution at your calibration temperature from the table on the standard bottle at the prompt and press enter For example if you are calibrating at 20 C the input value would be 7 02 Press enter The values of all the sensors will appear on the screen and change as they stablilize Step 8 Record the pre calibration reading in pH units on the calibration sheet Step 9 When the pH reading does not change significantly for 30 seconds press enter The calibration will be accepted Step 10 Record post calibration reading in pH units and the post calibration pH millivolts on the calibration sheet The pH millivolts value should be between 50 mV and 50 mV Press enter to continue Step 11 Return the sonde to normal orientation Empty the calibration cup Rinse the sensors and calibration cup once with de ionized water Step 12 Rinse the sensors and calibration cup three times with pH 10 solution Step 13 With the sonde inverted fill the calibration cup with pH 10 solution to immerse the pH bulb and the temperature sensor on the conductivity probe Step 14 Input the value of the pH 10 solution at your calibration temperature from the table on the standard bottle at the prompt and press enter For example if you are 23 calibrating at 20 C the input value would be 10 06 Press enter The values of all the sensors will appear on the screen and change as they stablilize Step 15 Record the pre calibration r
18. ame here Bat volts Battery voltage will show here e g 12 7 Volts Bat life Battery Life days Take note of this value This is the calculated battery life days based on the battery voltage and the number of measurements that will be taken given the 15 minute sampling interval With new batteries the programming will display a bat life of approximately 85 days This is only an estimate and assumes a temperature of 20 C The actual battery life may be several weeks less than this estimate so keep this in mind when deciding when to retrieve and download the sonde When the batteries run out of power the sonde will stop logging data Proceed to step 6 only if your sonde has already been calibrated If not calibrate the sonde prior to deployment Step 5 Select Start Logging and press enter to begin data collection Step 6 Disconnect the 650 and ensure that the cap is covering the electronics port where the field cable was removed Make sure only two threads of the communications communications connector are visible once the grey cap has been installed correctly If 29 more than two threads are showing after installing the cap water WILL get into the connection and potentially cause harm to the connector Step 7 Deploy the sonde in the river or lake being studied Make sure that it is well attached to a raft a buoy shore or preferably a combination After a Sonde Deployment Retrieving the sonde downloading the data a
19. ate Soe 16 Daterand IME A cakes et hee ee tee Meee ee Nae Se eee hee tee eee te eee eke 16 Barometer CalibratiGivonst cat cvcschetsvcectetveetckateuaicletotetctateuahstateaetctatatetclatectet aan 17 The Power Sonde Option reccrticseiett circ ttectiettiela eMac E a tietere tiie te oo 18 Sonde meU saia a cette tet eet Ea atte ie adie daaa aaa deai 19 Calibrating Sonde SSNSOl Siesta cass canon sees acrnsceoesaaeestacesceaveaerseuemoeedoees 19 Calibrating a clean or NEW sonde ccceceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeneaeeeeeeeeeeseeeenenaaees 20 Supplies required Jj iesccticetmdcc sodas apendsnaase cea piementcawntensandosenedpaetenedpiotend dewedenasuentens 20 Calibration IMSUUCHONG mec cn cpcocssnac ens coos ces iecteudachas ceveametiucisees inca cect cevasveienceatedtadiewatind 20 Te ISSO SUMO XV OEM chee AeA BE AERA AER EERE SA Aes a Oe 20 2 Depth eet peer ener a ne ee Weer eee ire ene Seer ere ere ee er eee ee eee eee een ee ere 21 3 Conductivity and Temperature ceeeeeeeceeneeeeeeeeeeeeeeeaeeeeeeeeeeeeseeeneeaaeeeeeees 21 POT A EEA TE tee tass sd asia Ss Cane hie AAEE EAE 23 5 Oxidation Reduction Potential ORP ccccceeeessseeecceeeeeeeeeeeeesseeaeeeeeees 24 PE G7 allo ge o ah A PE EE erage cee remanent ice Conran ea mEt iar fone one Comet SUM a ar Coane ae nie Ear 25 Poe TUNNEY ent cesses crac bees cece dese rele a a a eure cee mendes ramctaesc me 26 CANIS LAUIGMP SNC les setetias cet oe tet cnet TA 28 Logging
20. de sensors are measuring properly and generating accurate water quality data We basically have to train the sensors so that they know how to measure the parameters we are interested in Calibration requires solutions called standards with known levels of the chemical parameters we will be measuring We calibrate one sensor at a time The sonde sensors are immersed in the standards one standard at a time and the verified concentration of the chemical that is present is input into the instrument This way the instrument can link the signal it is measuring to the proper known concentration Then when the instrument measures an unknown level of the same parameter in the lake or river being studied it uses the calibration information to calculate the concentration of the chemical in the water It is critical that the sonde be calibrated before each deployment Calibrations of the different parameters measured by this sonde can involve using one single standard a one point calibration or 2 standards a 2 point calibration 19 Calibrating a clean or new sonde Supplies required Standard approximate volume required De ionized water 4 L Conductivity standard 1413 uS cm 1 413 mS cm 500 mL pH 7 standard 500 mL pH 10 standard 500 mL Turbidity standard 0 NTU 500 mL Turbidity standard 126 NTU 500 mL Note that this turbidity standard has two values depending on the YSI sensor being used The NWT sondes are using
21. e calibration sheet Press enter to confirm the calibration and zero the sensor Step 7 Record the post calibration chlorophyll reading on the calibration sheet Press any key to return to the Calibrate menu Press enter to continue 25 Step 8 Grab samples must be collected near the sonde at certain times during deployment and the Chlorophyll content determined in a lab Note the exact dates and times that these samples are collected These samples will allow the relative readings collected by the sonde to be expressed as real Chlorophyll concentrations 7 Turbidity Note turbidity requires at 2 point calibration Note turbidity must be calibrated with the sonde in the upright position Step 1 Ensure that the sensors and surroundings have been cleaned and are free of debris from past deployments Any solid particles will contaminate the standards during the calibration and cause the instrument to collect incorrect data Step 2 Pour enough of the 0 NTU standard slowly into the calibration cup avoiding bubbles so that the end of the turbidity sensor is submerged a few mm Engage only one thread when screwing the calibration cup onto the sonde to keep the sensor face as far away as possible from the calibration cup bottom The calibration cup itself is a source of interference Step 3 Reduce the lighting in room and wrap a black bag around the cup Step 4 From the Sonde menu select Calibrate press enter Step 5 Sel
22. e of moisture Use a clean lint free cloth to dry if necessary LUBRICATE O RINGS Ensure that the O ring on the sensor you are inserting is completely clean of dirt Dirt will allow a path for water to enter the electronics of the Sonde when it is deployed il init L Apply a very light sheen of Krytox to the clean O ring right aii before the sensor is installed to keep it in good shape Optical Sensors Install the optical sensors before you install the regular sensors Step 1 Insert the sensor into the appropriate port and gently rotate until the pins of the connectors are aligned Step 2 Slowly finger tighten the nut down toward the bulkhead During this step be careful not to cross thread the nuts If you meet resistance while finger tightening STOP back out and begin the process again Step 3 Finish tightening using the short end of the installation tool so that the seal is waterproof Do not overtighten When tightened properly it is normal for there to still be a 1 2 mm gap between the nut of the optical sensor and the body of the bulkhead Optical sensor Regular Sensors Install after the optical sensors Step 1 Insert the sensor into the appropriate port and gently rotate until the pins of the connectors are aligned Step 2 Push the sensor inward so that it seats in the port snugly Slowly finger tighten the nut down toward the bulkhead During this step be careful not to cross thread the nuts If
23. e value of the ORP solution in millivolts at the calibration temperature See the table in the instruction manual For example if we are calibrating at 20 degrees C the value to enter will be 237 5 mV Enter this value and press enter 24 TEMPERATURE CELSIUS ZOBELL SOLUTION VALUE MV fe Step 7 Monitor the stabilization of the ORP and temperature readings Once they are stable for approximately 30 seconds record the pre calibration ORP value in millivolts on the calibration worksheet If the probe is functioning within specifications the ORP reading should be within the range of 221 241 millivolts at normal ambient temperature Step 8 Press enter to confirm the calibration and record the post calibration ORP value in millivolts on the calibration worksheet Step 9 Press enter to continue and escape to return to the main menu Step 10 Return the sonde to normal orientation empty the calibration cup and rinse the sensors and calibration cup with de ionized water 6 Chlorophyll Step 1 Rinse the sensors and calibration cup thoroughly with deioinized water Submerge the chlorophyll sensor in de ionized water Step 2 From the Sonde menu select Calibrate press enter Step 3 Select Optic C Chlorophyll press enter Step 4 Select Chl RFU Zero press enter Step 5 Input the Chl RFU value of 0 at the prompt and press enter Step 6 When the readings have stabilized record the pre calibration chlorophyll reading on th
24. eading in pH units Step 16 When the pH reading does not change significantly for 30 seconds press enter The calibration will be accepted Step 17 Record post calibration reading in pH units and the post calibration pH millivolts on the calibration sheet The pH millivolts value should be between 165 mV and 180 mV below the pH 7 mV reading lf the difference is less than 165 mV or more than 180 mV the reference solution inside the probe may be too old DO NOT DEPLOY this pH probe The lifespan of reference solution inside the pH sensor is only 1 5 2 years Press enter to continue and escape to return to the main menu Step 18 Return the sonde to normal orientation Empty the calibration cup and rinse the sensors and calibration cup with de ionized water 5 Oxidation Reduction Potential ORP Note Calibrate ORP after calibrating pH Step 1 Prepare the Zobell calibration solution according to the instructions on the bottle Open the bottle and add 125 mL of de ionized water to the dry powder The solution has a shelf life of 6 months once mixed Step 2 Rinse the sensors and calibration cup with de ionized water and then some of the Zobell solution We have already completed these steps Step 3 Pour the Zobell solution into the calibration cup and immerse the pH ORP sensor and the temperature sensor in the solution Step 4 From the Sonde menu select Calibrate press enter Step 5 Select ISE2 ORP press enter Step 6 Enter th
25. ect Optic T Turbidity 6136 press enter Step 6 Select 2 Point press enter Step 7 Input the value 0 NTU at the prompt press enter The screen will display readings Step 8 Activate the sensor wiper 1 2 times by pressing 3 Clean Optics Step 9 When the readings have stabilized Record the pre calibration turbidity reading for 0 NTU on the calibration worksheet Step 10 Press enter to confirm the first calibration and record the post calibration value for 0 NTU on the calibration worksheet Step 11 Press enter to continue Step 12 Empty the cup and rinse the cup and sensors with DI water Dry both the calibration cup and the turbidity sensor Compressed air is handy for this step 26 Step 13 Pour enough of the 126 NTU standard slowly into the calibration cup avoiding bubbles so that the end of the turbidity sensor is submerged a few mm Engage only one thread when screwing the calibration cup onto the sonde to keep the sensor face as far away as possible from the calibration cup bottom The calibration cup itself is a source of interference Note that this turbidity standard has two values depending on the YSI sensor being used The NWT sondes use turbidity sensor model YSI 6136 Therefore the second turbidity standard value that you will need to input is 126 NTU Step 14 Reduce lighting in room and wrap black bag around calibration cup or jar Step 15 Input the value 126 NTU at the prompt press enter The screen
26. ep 2 Fill the calibration cup with Zobell s solution to immerse the pH ORP sensor and the temperature sensor on the conductivity probe Record the ORP reading in millivolts on the calibration sheet under after deployment reading 5 Depth Step 1 Record the pre clean depth reading in air on the calibration sheet under after deployment reading 34 Do not re use any of the standards used above to check for drift for any future sonde calibrations They have been contaminated by the algae dirt and grime present on the retrieved sonde Cleaning the Sonde After checking the after deployment readings clean the sonde according to the manufacturer s recommendation Use mild dish soap a soft toothbrush and the wire brush provided in the maintenance kit Rinse thoroughly with clean water after cleaning After cleaning ensure all sensors are properly rinsed with de ionized water Re Calibration and Re deployment Calibrate the freshly cleaned sonde as described above in the calibration section Set up logging and re deploy the sonde in the water body being studied Sensor Storage It is recommended to keep the sensors attached to the sonde after installation rather than removing them unnecessarily for storage The optical dissolved oxygen sensor should always be stored wet The pH ORP probe has a limited life and should be stored in pH electrode storage solution Potassium Chloride 3 5 M or in pH 4 buffer solution pH
27. ey Cellular Phone Style Keypad MS 8 Connector Strain Relief Lanyard The Arrow Keys are used to move up and down through the different selections in the menus The Enter key is used to confirm a selected menu item The Escape ESC key is used to return to the previous screen you were at in the menu The number buttons are used to enter words and data for example site names The following guide indicates the number of times you need to press each number button to access individual capital and lower case letters to spell out site names 13 1 1 2 ABC2abc2 3 DEF3def3 4 GHI4ghi4 5 JKL5jkl5 6 MNO6mn06 7 PQRS7pqrs7 8 TUVS8tuvs 9 WXYZ9wxyz9 0 0 If you are finding that the screen is difficult to read you can make the screen either darker or lighter by holding down the Backlight key and pressing either the up arrow to darken or the down arrow key to lighten Communication between the 650 display unit and the sonde Note Make sure only two threads of the communications communications connector are visible once the cable has been attached If more than two threads are showing after attaching the cable water WILL get into the connection and potentially cause harm to the connector When physically connected together using the field cable the 650 display unit and sonde can exist in two different communication states 1 In the first state the display unit is connected electronically
28. files in excel spreadsheet format change the txt file extension to csv and open with excel The 6600 sondes and 650 display units have a special type of memory called Flash memory Flash memory has an advantage because any recorded data is not lost if batteries die or the instrument malfunctions However a disadvantage is that you cannot delete individual data files from this type of memory You must delete reformat the entire memory chip to get back space in the memory once data has been downloaded Step 15 Select 6 Delete all files to remove all files from the sonde flash disk memory It is critical not to use this option until all data dat and calibration glp files from sonde memory have been transferred to your computer Every time you download data from the sonde and you are sure that it has been transferred entirely and properly to your computer erase the flash memory in the sonde and 650 display unit so that the maximum amount of memory is available for the next deployment After deployment check for drift After a deployment it is important to see if the accumulation of algae and dirt on the sonde fouling has caused the calibration of the sonde to change this is called drift Drift affects the data collected during the deployment and must be accounted for This 32 check for drift will be done by measuring standards as soon as the sonde has been taken out of the river or lake and returned to the lab It is impo
29. he 6067 calibration cable it plugged into and click OK Step 5 A terminal interface window like that shown below will appear If only a shows up in the window type menu and press enter The screen should now appear as shown below This is the main menu screen 2 EcoWatch Sonde COM1 File View Comm Realtime Appl Window Help ERNE it Run 2 Calibrate File 8 Advanced Select option for previous menu 1 Directory 4 View file 2 Upload 5 Quick view file 3 Quick Upload 6 Delete all files Select option 0 for previous menu 1 31 All data files stored in the sonde have a dat extension The file with the glp extension contains the calibration record of the sonde Step 7 Type 3 Quick Upload to transfer the entire last logged data file to your computer Step 8 Type 2 Comma amp Delimited The data file filename txt will now be transferred to the C ECOWIN DATA subdirectory of your computer Step 9 After the file transfer is complete Type 0 to return to the main menu Step 10 Type 3 File to return to the File menu Step 11 Type 2 Upload Step 12 Type the number associated with the glp file Step 13 Type 2 Comma amp Delimited The glp file as a txt file will now be transferred to the C ECOWIN DATA subdirectory of your computer Step 14 Confirm that the data and calibration files have been transferred to the computer and that they contain data To view data or glp
30. he dissolved oxygen sensor saturation to 100 Step 10 Record the post calibration dissolved oxygen in mg L and L Local on the calibration worksheet Press enter to continue Press Escape until you return to the main sonde menu Step 11 Record the ODO Gain value located under Sonde Menu Advanced Cal Constants on the calibration worksheet This value should fall between 0 75 1 25 If it doesn t meet this criteria the DO disc is probably old and in need of replacement Press Escape to return to the main menu Step 12 Empty the calibration cup and rinse with DI water 2 Depth Step 1 Make certain the depth sensor is in air and not immersed in any solution Step 2 From the Sonde menu select Calibrate press enter Step 3 Select Pressure Abs press enter Step 4 Input 0 00 and press enter Step 5 Wait 30 seconds until the readings have stabilized Record the pre calibration depth reading on the calibration sheet Step 6 Press enter to confirm the calibration Step 7 Record the post calibration depth reading on the calibration sheet Step 8 Press enter to continue and then escape to return to the main menu 3 Conductivity and Temperature Step 1 Rinse the sensors and calibration cup once with de ionized water Step 2 Rinse the sensors and calibration cup 3 times with conductivity solution 1413 uS cm 1 413 mS cm at 25 C 21 Step 3 Screw the calibration cup onto the end of the inverted Sonde i e
31. he sonde can be deployed will be incorrect and it is possible that the sonde will not be powered and will not store data when deployed It is best to just ensure that both the display unit and sonde have good batteries in them at all times and leave the Power sonde option OFF no checkmark at all times Sonde menu Now that we have set up the 650 display unit it is time to connect the display unit electronically to the sonde From the 650 Main menu select Sonde menu A box with the word connecting will appear quickly and then disappear The small picture in the bottom right hand corner of the 650 display will change to show a picture of the sonde You are now in the menu system for the sonde In this series of menus you can calibrate the sonde and change sonde settings You will now see the following menu The first screen you will see is the Main menu for the sonde Do not Ma in Run o O confuse this menu with the main Calibrate menu for the 650 display unit File Orient yourself using the icon in Status the bottom right corner if System necessary You can exit the sonde agi SondeIcon menu system from this screen by Aana pressing the escape key The sonde will disconnect electronically and you will be returned to the main menu of the 650 display unit N 0 0 000 E 0 0 000 740 4m9 n 05 31 2000 14 21 14 Qmm Calibrating Sonde Sensors Calibration is an important process that helps the user ensure that the son
32. ics inside the Sonde are easily damaged by water It is very important that water and other solutions are NEVER allowed to enter the PVC body of the sonde Ensure that all sensors plugs and the cap that covers the cable port are properly installed at all times It is very important to maintain the rubber O rings on the sensors and caps Whenever a sensor plug or cap is removed for cleaning or servicing dirt on these O rings should be cleaned away gently Before sensors plugs or caps are replaced put a little dab of Krytox included with the Sonde on the O rings just use enough so that it appears shiny NOT goopy O rings should be replaced every one to two years to ensure they keep a good watertight seal Make sure the batteries inside the sonde have sufficient charge before deployment see discussion of calculated sonde battery life on page 27 of this manual The sonde cannot collect data without battery power The end of the optical dissolved oxygen sensor must always be stored in a moist environment when it is not in use If it is detached from the sonde it should be stored with a moist sponge inside its flexible plastic black cap The pH ORP probe has a limited life To make the probe last as long as possible the end of the pH ORP probe when attached to the sonde should be stored long term in pH electrode storage solution Potassium Chloride 3 5 M or in pH 4 buffer solution If the probe is not attached to the sonde dur
33. ing storage it should be stored in the electrode storage solution from the manufacturer The end of the pH ORP probe is extremely fragile It is made up of two glass bulbs that are easily broken Never care when using the calibration cup and probe guard so pez rest the sonde on the end with the sensors Always take that you don t knock them against the ends of the sensors Initial Setup Installing Batteries in the Sonde Batteries provide the power for the sonde to collect and store data when it is deployed in the water Make sure that the sonde contains fresh batteries before it is deployed or no data will be collected This sonde takes 8 C type alkaline batteries Carry spare batteries Step 1 Using the hex tool loosen the battery lid screws Step 2 Remove the lid and install the batteries as shown to the left Ensure that they have the proper orientation Step 3 Before replacing the lid it is important to ensure that the orange O ring is seated completely within the groove on the underside of the lid Press around all its edges to make sure it is not popping out of the groove anywhere along its length Small retaining tabs along the O ring help keep it inside the groove There is a detailed description of how to properly seat this orange O ring on pages 2 19 to 2 20 of the YSI instruction manual Do not lubricate the orange O ring but make sure it is clean Step 4 Make sure that the black O rings that enc
34. ircle the lid are completely clean No dirt should be present on or under any of these O rings Apply a small amount of Krytox to the black O rings so that they are shiny Step 5 Put the lid back on the battery compartment in the orientation showed to the left and tighten the screws until snug Do not overtighten the screws If the batteries have been properly installed the voltage should read 12 0 volts or higher in the sonde Status Menu Remove batteries from the sonde when not in use i e for long term storage The battery compartment of this sonde has alid valve with a round pressure release safety valve In iy the highly unlikely event that the batteries a should malfunction and de gas this valve will release any pressure building up in the battery compartment If the valve ever opens the battery compartment lid must be replaced with a new one from the manufacturer before the sonde can be used again Battery compartment lid Installing Sensors To prepare the sonde for calibration and data collection you need to install probes also called sensors into the ports on the sonde bulkhead When initially received from the manufacturer there are screw in plugs in the ports to protect the port connections from damage Do not discard these plugs Save all plugs for future use Step 1 Remove plugs from ports Plugs are removed by inserting the long end of the installation tool into the holes
35. nd checking for drift Sonde retrieval Upon arriving at the sonde take note of site conditions such as temperature precipitation in last 24 hours flow color of water unusual odors siltation material in bottom of river and any other observation you deem important Record all this information in your field book Remove the sonde from the water body If the sonde is still logging data hook up the field cable and 650 display unit and connect to the Sonde Menu Select Run Unattended Sample and then Stop Logging Return to the lab Downloading Water Quality Data from the Sonde to a Computer Step 1 Install the EcoWatch software on your computer Do this by placing the CD in your computer s CD drive An InstallShield Wizard will automatically start on most computers Follow the prompts to complete the installation Step 2 Clear any debris from the bulkhead connector cap If there is water present dry the electrical connections thoroughly before attempting any connections Remove the cap and connect the calibration cable YSI 6067 pictured below to the sonde and to your computer s com port CAUTION The 6067 calibration cable is for laboratory use only itis not waterproof and should never be submersed 30 Step 3 Select the EcoWatch icon on your computer desktop and open the software Step 4 Click Comm on the toolbar Step 5 Click Sonde on the drop down menu that appears Step 6 Select the appropriate com port that t
36. obe Guard and Calibration Cup pg 10 14 52 Connecting the Sonde to the 650 MDS Display Unit pg 11 17 23 Calibrating Dissolved Oxygen pg 20 22 34 Calibrating Depth pg 21 24 16 Calibrating Conductivity and Temperature pg 21 33 32 Calibrating pH pg 23 44 54 Calibrating ORP pg 24 47 51 Calibrating Chlorophyll pg 25 49 47 Calibrating Turbidity pg 26 36
37. r the data are downloaded to a computer After routine maintenance and re calibration the sonde can then be re deployed to collect more data A sonde is different than a hand held meter Hand held meters are portable and are used to collect one or a few measurement at a site Sondes are deployed at a location for a long period of time and collect many measurements Field cable Sonde bulkhead There are some advantages to using a Sonde over traditional water quality sampling methods such as manually collecting samples and sending them to a lab For example the Sonde can collect a lot of data more frequently with less effort and at a lower cost than traditional sampling Of course there are disadvantages to using a Sonde as well The Sonde can only measure a few key parameters whereas a water sample that is collected and sent to a lab can be analyzed for a much broader suite of parameters Manufacturer name and model number of this Sonde YSI 6600 What parameters does the sonde measure This particular sonde measures the following parameters in water 1 Temperature in degrees Celcius 2 Conductivity 3 pH 4 Oxidation Reduction Potential ORP 5 Dissolved Oxygen 6 Turbidity 7 Chlorophyll For general background information on these parameters and why it is important for us to measure them please see the attached Water Quality Fact Sheets Important Points to Remember 1 oo Z 2 The electron
38. rtant not to clean the sonde until these checks have been completed It is also important not to rinse the sensors too much during these steps to get an accurate measurement of the fouling effect Supplies needed to check for drift Standard volume required De ionized water 4 L Turbidity standard 0 NTU 500 mL pH 7 500 mL pH 10 500 mL Conductivity standard 1413 uS cm 500 mL Zobell s solution 500 mL Calibration sheet Sonde maintenance kit Protective gloves wear these during all steps Instructions checking for drift 1 Turbidity and DO Step 1 Put de ionized water 0 NTU into the calibration cup so that only a few mm of the turbidity sensor are in contact with the water with sonde right side up Step 2 Screw the YSI onto the calibration cup only engaging a few threads so air can diffuse into calibration cup Step 3 Activate the turbidity wiper reduce lighting in room and wrap black garbage bag over calibration cup to reduce lighting Step 4 Record the turbidity reading on the calibration sheet under after deployment reading Step 5 Remove the bag Pour out the 0 NTU standard until there are just a few mL left in the calibration cup Ensure that the DO sensor and temperature sensor do not have water droplets on them They should both be dry and neither should be submerged in the standard Re attach the calibration cup engaging only a few threads so that air can diffuse into the cup Wait
39. you meet resistance while finger tightening STOP back out and begin the process again Step 3 Use the long end of the installation tool to tighten the nut an additional quarter turn until snug Do not overtighten When tightened properly there should be no space between the nut of the regular sensor and the body of the bulkhead Installing Probe Guard or Calibration Cup The probe guard protects the sensors he during deployment and allows water se to flow over the sensors Once all the es probes are correctly installed and ia a calibrated screw the guard onto the 5 sonde by aligning the threads and turning clockwise until the guard is securely attached Pi The calibration cup is used during sensor calibration and for long term storage when the sonde is not in use It is a solid plastic cup The calibration cup is installed in exactly the same way as the probe guard 10 YSI 650 MDS Display Unit The YSI 650 MDS display unit is an instrument that allows the user to tell the sonde what parameters to measure and how often to make water quality measurements when it is deployed in water The display unit is also used during calibration of the sonde Installing Batteries in the 650 Display Unit The YSI 650 display unit requires 4 C type batteries Change the batteries in a dry environment Carry spare batteries Step 1 Loosen the 4 screws on the battery compartment lid using a screwdriver and remove the lid completely
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