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SBC CyclePO4=3=Hach A4 Portrait User Manual - Sea

1.
2. Test Flag Value from Description sensor Bubble spike Suspect 355 826 over Max Good of 300 but less than Max Suspect of 500 counts COV Good 0 between Min Good of infinity and Max Good of 2 5 Low signal Good 2 954 between Min Good of 720 and Max Good of infinite counts Out of range Good 2 024 between Min Good of 0 075 and Max Good of 10 5 uM Mixing spike Bad 250 over Max Suspect of 100 counts refer to graph above Cal spike Bad 441 over Max Suspect 100 counts refer to graph above 69 Reference 70 Section 5 General information 5 1 Warranty Revised editions of this user manual are on the manufacturer s website This sensor is warranted against defects in materials and workmanship for one year from the date of purchase The warranty is void if the manufacturer finds the sensor was abused or neglected beyond the normal wear and tear of deployment 5 2 Service and Support The manufacturer recommends that sensors be sent back to the factory annually to be cleaned calibrated and for standard maintenance Refer to the website for FAQs and technical notes or contact the manufacturer for support at support sea birdcoastal com Do the steps below to send a sensor back to the manufacturer 1 Contact the manufacturer for a Return Merchandise Authorization RMA Note The manufacturer is not responsible for damage to the sensor during return shipment 2 Remove all anti fouling treatment from the
3. sensor sends 000007 lt CR gt lt LF gt SDI recorder sends OCC sensor sends 000007 lt CR gt lt LF gt Send Data commands that come after either the aM or aMC commands Send Data aDO aD1 Response a lt values gt lt CR gt lt LF gt or a lt values gt lt CRC gt lt CR gt lt LF gt Purpose sends data to the SDI recorder Notes aDO after aM 35 characters are allowed For the sensor the response is 6 the first 6 values in the SDI data format table There is not space in the 35 character field for 7 values If a subsequent aD1 command is sent the sensor will send the seventh value battery voltage aDO after aC 70 characters are allowed For the sensor the response is 7 values There is space in the 70 character field for 7 values The recorder does not need to send an aD1 command because all 7 values are sent after the aDO command aDO and aD1 after an aM command Assume the sensor is set to address 0 Example 1 address 0 values from the previous measurement sample date 11 0705 sample time 06 0708 run number 0500 PO concentration 12 345 units of PO concentration 0 last sample state 9 battery voltage 12 1 SDI recorder sends OM sensor sends 00007 lt CR gt lt LF gt SDI recorder sends ODO sensor sends 0 11 0705 06 0708 0500 12 345 0 9 lt CR gt lt LF gt recorder sends 0D1 sensor sends
4. 2 1 3 2 Prime sensor with vacuum 1 10 2 1 3 3 Fill sensor filters 1 Attach the Luer lock and the 1 16 ID barb adapter to the exhaust tubing and to the supplied syringe Attach the syringe with the adapter to the outlet of the 1 16 ID sensor effluent tubing that comes out of the top end flange Under the Settings tab make a check in the Cal box and set the calibration pump to operate for 100 pump cycles Toranennd Num Pumps 100 Run Pump s Stop Push Run Pump s While the pump is in operation pull a light vacuum approximately 1 5 10 mL of the full travel of the plunger After the pump has operated for 100 cycles make sure that the reagent tubing that connects the cartridges and the inlet barbs does not have any air bubbles Look at the tubing from the reagent cartridges to the manifold to check for bubbles If bubbles are present do steps 4 and 5 again If bubbles are small it may not be possible to remove them Do steps 3 5 with R1 and R2 Disconnect the tubing from the syringe Put the end of the tubing into an empty receptacle Go to the Settings tab and push Flush The Sample fluid opening is primed Fill the filters with Dl water 1 Disconnect the 1 8 ID tubing that connects the filter to the S inlet barb if necessary 2 Connect the manufacturer supplied syringe to the 1 8 ID tubing Push clean water into the filters 3 Pinch the tubing and remove it from the
5. Stop after next current sample Cycle On Board Data Storage Dir On Board Disk Storage Free Space 950 304 768 Used Space 97 058 816 Total Space 1 047 363 584 Refresh Root Directory Listing Offload Selected File s Dir Replay Selected Files Cancel Offload Refresh Delete Selected File s Dir s Create New On Board Dir Hide Offloaded Current File o Operation 4 Enter the file directory or folder on the PC to save the data from the sensor or create a new fol der Host Computer Offload Dir C CycleDatalRuni C CycleData Data Status PO4 Plot Raw Plot E DatalRuni ILJ 5 Push Offload Selected File s Dir to move a copy of the data from the sensor to the PC The user can save only one directory at a time but it is possible to select several files at the same time to save to the PC Monitor the data saved to the PC Look at the Current File area at the bottom of the software window 23 Operation 2 5 2 Operation sequence Counts This section describes how the sensor calculates phosphate and how to interpret the quality of the data 1 2 000 i l coreo ore j j Cee r emea 4 5 6 8 9 3 7 Table 2 Cycle output periods 1 Blue Pre analysis flush period The sample pump operates Referred to as the baseline 2 Red Ambient read period Used for 100 transmittance without any absorption from phosphate reaction
6. 4 Tie a safety line from the stainess eyes at the top of the instrument to a secure structure How to thread the webbing through the buckle ull to tighten SY WET Labs 33 sewn end free end Cycle mounting bracket Replace any questionable hardware that is less expensive than the data from the sensor Make sure screws screw eyes brackets ropes straps zinc anodes etc are in good condition Replacement parts are available from the manufacturer or a marine supply store The sensor effluent exits through the outlet tubing Make sure the effluent flows freely and does not go onto the sensor or its mounting The effluent contains antimony and molybdenum and has a pH of lt 2 Make sure to wear the proper Personal Protective Equipment PPE to work near this effluent Refer to the MSDS that comes with the reagent cartridges for specific information Obey local state and federal laws to dispose of waste Contact the manufacturer for waste containment solutions 2 3 2 1 SDI operation 16 All sensors that have an 8 pin connector can operate on an SDI 12 network SDI 12 version 1 3 is supported Refer to the SDI 12 Version 1 3 specification at http www sdi 12 org for details Required equipment 1 Cycle sensor with both 6 and 8 pin connectors 2 PC with Cycle host software installed 3 SDI recorder Operation 4 Power supply 5 6 socket test cable 6 8 socket SDI cable Power requirements and sample setups T
7. RS232 RX No connect OINI DoD oa BR Ww bd No connect 1 2 Electrical Main port 6 pin SDI port 8 pin Input 10 5 18 0 V Current draw operation 115 mA avg 2 A max 125 mA avg 2 A max 10 mA SDI 12 Current draw low power 30 yA Power dissipation 1 15 W avg 12 W max Communication interface RS232 ASCII SDI 12 Baud rate 9600 115 200 1200 baud Data storage 1 GB compact flash card type II Specifications External wake high voltage 3 5 20 0 V External wake low voltage 0 0 3 3 V 1 3 Optical Wavelength 870 nm Pathlength 5 cm Linearity gt 95 R 1 4 Analytical Detection limit three standard deviations of 18 MOhm water less than or equal to 0 075 uM less than or equal to 0 0023 mg L PO P Quantification limit ten standard deviations of 18 MOhm water less than or equal to 0 025 uM less than or equal to 0 0077 mg L PO P Range nominal 0 10 uM 0 0 3 mg L PO4 P A higher range of 0 40 uM 0 1 2 mg L PO P is possible but outside specifications Section 2 Operation 2 1 Sensor setup 2 1 1 Assemble the sensor Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses The Cycle PO sensor comes in two boxes One is a fiberboard box with the reagent cartri
8. The range is 5 to 4924 Response The new or current idle timeout in seconds INT sampling INTerval Description Get set the time period between data samples referenced from one start time to the next Argument 1 The interval in seconds or minutes seconds or hours minutes seconds If 0 the sensor will collect one data sample and then stop Response The new or current sample interval as hh mm seconds NOS Number of Samples Description Get set the number of data samples includes the current data sample to collect before the sensor stops Argument 1 The number of data samples to collect The default is 1 which sets no limit to the number of data samples Response The new or current number of data samples to collect OPD Output PerioD Description Get set the data output interval Use with SDO command that looks like the old sensor data output format to specify how often a data sample is sent from the sensor The default is 5 This value also specifies how often the raw signal data is written to the raw data files Argument 1 The new output interval Makes the old version data output to show every nth LED cycle Response The new or current period of output 41 Reference RAT Serial port RATe Description Get set the baud rate for the serial port Argument 1 The new rate Values are 9600 19200 38400 57600 and 115200 Response
9. frequency These will start measurements on a preset schedule The sensor will ignore the aM and aC commands if a prime sequence is scheduled but not complete The sensor is primed before it starts to collect data The SDI schedules when to collect data The sensor controls whether the data measurement is spiked or normal Schedule the aM or aC commands at an interval longer than 35 minutes spiked measurements take approximately 35 minutes 17 Operation 2 3 3 Deployment procedures Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses The user makes the decision about which mode of operation to use then does the steps below to deploy the sensor 1 Install new cartridges on the sensor Refer to the sections on Assemble the sensor on page 5 Prepare to prime the sensor on page 9 and Prepare sensor for deployment on page 12 for details 2 Connect the sensor to a 12V 15 watt power supply and PC with the manufacturer supplied cable The user needs a serial to USB adapter for the supplied cable to connect the sensor to the PC 3 Start the Cycle software and choose the applicable serial port 4 Turn on the power supply to the sensor 5 Push Get Settings to make sure that the software and the sensor have communication 6 Select the Tools menu then Deployment Wizard 7 Choose the desired mode to operate the sensor a
10. to keep the optics clean from the products of chemical reactions which can cause a decrease in sensitivity Both solutions are available from various scientific supply companies 1 Make sure that the sensor is connected to a power supply and a PC with the Cycle software on 2 Pull the sample tubing straight off the hose barb to disconnect Figure 18 Sample tube loosened 3 Unwrap the exhaust tubing from the top of the sensor and put one end into a beaker 4 Connect a syringe to a 25 cm length of 1 8 inside diameter ID Tygon tubing 5 Pull a minimum of 10 mL of 2 cleaning solution into the syringe Figure 19 Micro 90 pulled into syringe Sane e Oe 6 Connect the other end of the tubing to the S barb Figure 20 Tubing connected to sensor 7 Inject the contents of the syringe into the tubing 32 Maintenance Figure 21 Tubing in cleaning solution AA SS r nde a dl a Disconnect the syringe and put the tubing it was connected to into the bottle of 8 cleaning solution 9 Go to the Settings tab of the Cycle software and push Flush The sensor takes 5 minutes to fill with the cleaning solution 10 Let the solution soak in the sensor for approximately Y hour to 1 hour 3 3 1 Clean flow paths with bleach solution Bleach is caustic Wear nitrile gloves and safety glasses and work in a well ventilated area to use bleach Never mix bleach with ammonia lt can make dan
11. 0 12 1 lt CR gt lt LF gt 52 Reference aDO and aD1 after an aMC command Example 2 address 0 values from the previous measurement sample date 11 0706 sample time 06 0809 run number 0501 PO concentration 12 678 units of PO concentration 0 last sample state 9 battery voltage 12 0 SDI recorder sends OMC sensor sends 00007 lt CR gt lt LF gt SDI reco rder sends ODO sensor sends 0 11 0706 06 0809 0501 12 678 0 9GTu lt CR gt lt LF gt recorder sends 0D1 sensor sends 0 12 0GFS lt CR gt lt LF gt aDO after an aC command Example address 0 values from the previous measurement sample date 11 0707 sample time 06 0910 run number 0502 PO concentration 12 901 units of PO concentration 0 last sample state 9 battery voltage 11 9 SDI recorder sends OC sensor sends 00007 lt CR gt lt LF gt SDI reco rder sends ODO sensor sends 0 11 0707 06 0910 0502 12 901 0 9 11 9 lt CR gt lt LF gt aDO after an aCC command Example address 0 values from the previous measurement sample date 11 0708 sample time 06 1011 run number 0503 PO concentration 12 234 53 Reference units of PO concentration 0 last sample state 9 battery voltage 11 8 SDI recorder sends OCC sensor sends 00007 lt CR gt lt LF gt SDI reco
12. 1 500 1 750 Wed 08 05 15 22 55 59 1 Thu 08 06 15 05 56 01 15 Thu 08 06 15 06 26 00 16 Thu 08 06 15 06 56 01 17 Thu 08 06 15 07 26 00 18 Thu 08 06 15 07 56 00 19 00 00 00 00 02 00 00 04 00 20000004 TxT Qa 20000004 TXT 00 10 00 00 12 0 Look at the summary graph in the QC Plot tab of the software Each of the files that were selected to offload shows as a green yellow or red dot green dot the file has no suspect or bad test parameters yellow dot the file has one or more test parameters that are suspect red dot the file has one or more test parameters that are bad blue dot the data is saved for analysis but the related raw data file has not yet been read for analysis The blue dotted line shows which graph displays in the Analysis Plot window 2 6 2 Compare data files Use the information in the QC Plot tab to compare data files and make analyses of each file Use the values of the test parameters to help make a decision about the quality of the data 1 Y Show out of range data I Show spike plot Clear Save Analysis Set I compress timescale I Show VAPO4 Sample Thu 08 06 15 08 26 00 20 CAPO4 2 063 uM VAPO4 1 553 uM Bubble Spike Good 217 178 cov Good 0 888 Low Signal Good 2 959 Out Of Range Good 2 063 Mixing Spike Suspect 79 Cal Spike _ Not Evaluated 9 If necessary open the files to examine Refer to the previous secti
13. 2 4 2 In laboratory sensor stability analysis 20 1 Select the Raw Plot tab to see the data 2 Look at the data after 19 operation cycles to see if the data is stable Operation Figure 14 Example of stabilized data Counts If the data is stable go to the next section If the data is not stable refer to Prepare to prime the sensor on page 9 and do the steps again to make sure the sensor is set up correctly and has not pulled in any air which will give data that is not accurate Figure 15 Bubbles the sample line junts Co coco 0001100 000200 090300 000400 020500 000500 21 Operation Figure 16 Shifting baseline Counts 2 4 2 1 Use of water tanks for in laboratory performance analysis When the sensor operates in a laboratory water tank temperature changes or a decrease in water flow can cause air bubbles to form The manufacturer recommends that the user operates a pump to circulate the water that crosses the sensor intake areas to prevent the collection of air bubbles Data output values may change because of adsorption or primary production of a water tank The manufacturer recommends that the user validates the water in the tank 2 4 3 NIST check standards for in laboratory performance analysis The manufacturer uses a 5 3 UM NIST traceable check standard that is used after calibration and before servicing to check the sensor s calibration This 5 3 uM check standard is al
14. 2 960 Good Out Of Range Si Clear Save Analysis Set Analysis set statistics for 26 samples 66 8 5 15 7 JO PM 8 6 15 I JO AM 8 6 15 7 JO AM 8 6 15 Lo PM Date Good Suspect Bad Missing Data I Show VAPO4 Current File X Axis Scaling Time and Date Data is automatically scaled to show all of the data files that the user has selected to show in the Raw Data Plot window Sample Order Data shows by sample number which agrees with the sample number shown in the list of user selected data files in the Raw Data Plot window Selected Sample Information Sample The day date time and the sample number of the total number of samples of data collected by the sensor e CAP04 The phosphate value in uM that is calculated by the software e VAP04 checkbox The sensor calculated spike PO values in the summary graph The VAP04 values show as squares Summary table for specific sample Shows the name of the test and the status and value of the data collected Reference Sample Wed 08 05 15 22 55 59 1 CAPO4 2 024 uM VAPO4 1 350 uM tes rel vawe Bubble Spike Good 75 186 COV Good 0 Low Signal Good 2 954 Out Of Range Good 2 024 Mixing Spike Bad 250 Cal Spike Bad 441 e Clear Push to erase the files that were loaded by the software that show in the right side of the Raw Data Plo
15. 300 500 counts Bad above 500 counts Coefficient of Variation COV the software calculates the statistical coefficient of variation of the last ten values of transmittance data at the end of the initial flush period in the sample sequence The values show as a percentage Any change in the optics usually caused by nano micro bubbles in the baseline can cause the data in this period to be non flat The user may change this value if for example the flush1 data agrees with grab samples to a maximum good of 5 5 and maximum suspect of 10 67 Reference 68 Good less than 2 5 e Suspect 2 5 7 5 Bad above 7 5 Out of Range The software monitors if the data that is output is in a reasonable range The detection limit of the sensor is approximately 0 075 uM 2 ugP L Data that is collected near the detection limit can scatter at approximately O and users have collected good data that is below the detection limit so the manufacturer permits negative values to 0 05 uM 5 ugP L Values that are lower than that are bad The user may change this value for example to make all negative data bad change Min Sus to 0 or to accept data that is above the Max Good of 10 5 uM change Max Good to 20 e Good 0 075 10 5 uM often linear e Suspect 0 05 0 075 uM 10 5 40 uM Bad less than 0 05 uM over 40 uM the detector receives very little light so phosphate cannot be measured Low Signal the software calculates
16. Cycle software files will unzip Go to the Cycle Host folder Double click on the CycleHost exe file Operation OL wr gt oa gt AZ 4 WETLabs 4 CycleHost J JRE de lib dy Windows Defender do Windows Mail Organize v Include in library y Sharewith gt fl gt Name J RE J lib E CycleHost exe 10 If a Windows security warning shows push Run to continue to install the Cycle Host software r Open File Security Warning run this software Eri Name La Publisher Type From The publisher could not be verified Are you sure you want to 7 Always ask before opening this file This file does not have a valid digital signature that verifies its y publisher You should onty run software from publishers you trust How can decide what software to run tam Files x66 WETLabs CycleHost CycleHost exe Unknown Publisher Application C Program Files 86 YWETLabs CycleHost Cycle Run Cancel 2 1 3 Prime the sensor Note Use a vacuum only to prime the sensor Do not use pressure 2 1 3 1 Prepare to prime the sensor Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses The sensor comes with de ionized DI water in all of the fluid passages The user must prime the sensor before it is turned on This will move the
17. If argument 1 is used the response is Changing rate to arg1 Hit lt Enter gt when ready If no argument is used or after lt Enter gt is pushed the new baud rate shows Note that data samples collected at a baud rate other than 19200 may result in bad data The baud rate cannot be changed while data is being collected SDA SDI Address Description Get set the SDI bus address for the sensor Argument 1 The new SDI sensor bus address as an integer from 0 to 9 Response The current or new address This will also show an address change that was sent via the SDI bus SDO Output mode Description Get set the format of the data that is output The default mode of 0 is the same as the old data output format with final engineering units added at the end of each sequence Modes 1 and 2 are for use by the manufacturer Mode 3 shows only the engineering units of data at the end of a data sequence Mode 4 shows no engineering units data Argument 1 The mode of operation to use Response The current or new mode of operation SPR Set PRompt Description Turn the command prompt of PO4 on or off Argument 1 1 turn on command prompt 2 turn off command prompt Response The current or new command prompt shows as either on or off When the prompt is turned off there is no prompt after a response When the prompt is turned on there is a PO4 prompt after a response STO STOre configuration to flash Description Saves the current configuration values
18. No pumps operate 3 Green Sample mix period The sample pump and both reagent pumps operate 4 Purple Sample read period No pumps operate The reaction curve color develops Counts decrease until complete The white circle and number show the signal used as the sample transmission 5 Blue Post analysis flush period The sample pump operates Output counts spike then increase to approximately the baseline value 6 Red Spike ambient output period No pumps operate 7 Green Spike mix period The sample pump both reagent pumps and the calibration standard pump operate A known amount of phosphate is added to the sample 8 Purple Spiked sample read period No pumps operate Signal output counts decrease because there is more phosphate added to the sample This means more color develops and the transmission is lower 9 Blue Final flush period The sample pump operates As with the other flush periods the output returns to a baseline value 2 5 3 Blank run example 24 A clean sensor will usually have a decrease in counts as it is conditioned When the user calibrates the sensor there is a shift in ambient read counts from run to run or a slight shift in the pre and post analysis rinse baseline of 50 100 counts Operation Counts 000000 00 02 00 000400 co ceco 000800 00 1000 001207 00 1400 001800 00 1800 ime 2 5 4 Good quality calibration data Data such as the seven overlaid lines below shows good data The lines show f
19. air A 500 mL bottle will be enough solution for approximately 10 sample cycles csf 1 This will make a little more than 500 mL of waste Make sure the waste container is large enough for this volume Use deionized DI or tap water until the data collected by the sensor is stable Note DI and tap water can contain measurable phosphate e Use ultrapure Millipore or equivalent 18 MOhm water to prepare the check standards and the blanks Refer to the sections below to analyze the performance of the sensor in the laboratory 2 4 1 Setup for in laboratory performance analysis 1 Make sure that the sensor is connected to the host PC and a power supply and is in standby mode 2 Make sure to have 1 L of clean water with no particles over 10m 3 If necessary start the Cycle software 4 Goto the Settings tab and push Get Settings Operation B Cycle Host s n 104 File Tools Help COM Settings Time and Date Settings Status Sleeping COM 5 Get Settings PST MM DD YY HH MM 55 Time to next sample Host 01 05 12 08 37 53 Time to Prime Rae _Apply New Settings Cycle orpospizos 37 51 Run stop Step Clear Changes E Stop after next current sample Host Computer Offload Dir C CycleData v m Status PO4 Plot Raw Plot E Hi Monitor Files NA o 10 11 12 13 14 Set Date and Time Units uM v L Prime Now Sample Settings Deployment Calculator Estimates Current Propo
20. from the top of the two stainless steel filters Remove the two screws that hold the intake filter holder to the base plate Remove the filter housing from the base plate Loosen the set screw that holds the intake filter in the holder Push gently on the tubing fitting of the filter to remove the filter from filter housing Remove the plastic spacer from the bottom of the filter Remove the copper screen from the base plate Replace the copper screen with the new screen that came with the filters Install the plastic spacer onto the bottom of the new filter 10 Put the new filter into the filter holder Figure 23 10 um filter installation M Y A correct height lt E PA 11 Tighten gently the set screw that holds the filter in place Do not over tighten 12 Install the filter and the holder onto the base plate a Start one screw Hold the other side of the filter holder stable with a thumb or finger Start the second screw c Make sure the screws are tightened evenly Try to keep the filter holder parallel to the base plate d Tighten to hand tight Do not tighten too much 3 7 Prime sensor with vacuum 1 Attach the Luer lock and the 1 16 ID barb adapter to the exhaust tubing and to the supplied syringe Attach the syringe with the adapter to the outlet of the 1 16 ID sensor effluent tubing that comes out of the top end flange Under the Settings tab make a check in the Cal box and set the cal
21. sensor is in a low power mode Idle The sensor is not in operation and has not yet automatically gone to a low power mode If the sensor does not receive a command within 2 minutes the default idle time it will go to low power Running The sensor is in data collection mode or in a flush mode Pumping The pumps are in operation The manufacturer recommends the user use the Run Stop and Sleep buttons in the laboratory Use the Deployment Wizard for field deployments 59 Reference 4 3 9 1 Operation options Operate the sensor or set a time for the sensor to operate with the Choose Run Option window 2 7 Choose Run Option Set Start Time Date Run Now Cancel Option Action Set Start Time Date Select the start times and dates to set up and operate the sensor Run Now The sensor starts to operate Cancel The window closes 4 3 9 2 Stop options The user has two options to stop the sensor Stop Sampling 2 Choose a stop option u Stop and Flush Stop Now Options Action Stop and Flush Stops the sensor and starts a flush sequence of approximately 5 minutes Do this at the end of a sample sequence Stop Now Stops the sensor The manufacturer recommends this only if the user starts a new sample sequence soon after the sensor is stopped Continue Closes the window The sample sequence will not stop 4 3 9 3 Low power option Put the sensor into a low
22. syringe 11 Operation 4 Connectit to the S barb again to prevent the loss of prime 5 The filters will drip some water after this step It is not possible to remove all of the air bubbles Try to remove as many as possible 2 2 Prepare sensor for deployment Use the host software to make sure that the sensor is in a low power sleep mode Disconnect the test cable from the sensor the power supply and the PC Wind the exhaust tubing at the top of the sensor Make sure to align the indentations of the protective sleeve with the eye bolts then slide the protective sleeve over the sensor Pen gt Figure 13 Protective sleeve aligned with eye bolts oe 5 Put the sensor on its side hold the eye bolts and align the screw holes for the sensor and the protective sleeve Note that the protective sleeve is longer than the sensor The screws cannot be installed when the sensor is vertical 6 Install the seven Phillips screws again 7 If necessary start the Cycle software 8 Make sure that the sensor is connected to the host PC and a power supply and is in standby mode 9 Go to the Tools menu 10 Select the Deployment Wizard Fie Tools Help 0 Deployment Wizard CtrtD Get Settings Ctrl G Options Ctri O Restore Instrument Settings 11 The Cycle Deployment Wizard window will appear 12 Operation 12 13 14 15 16 17 18 19 20 21 22 23 24 Cycle Depl
23. taken SDI recorder sends aPRIME sensor sends PRIME Y lt CR gt lt LF gt Prime aX_PRIME Response aPRIME Y lt CR gt lt LF gt or aPRIME N lt CR gt lt LF gt depends on prime status Notes this command sends the status of the prime sequence Y or N 55 Reference aX_PRIME Example address 0 a prime sequence is occurring SDI recorder sends OX_PRIME sensor sends OPRIME Y lt CR gt lt LF gt the prime sequence starts 4 3 Software reference 4 3 1 Engineering units output Table 9 Data output format Field Description mm dd yy Date hh mm ss Time Run Run counter Increases in increments of one for each sample sequence CAP04 The phosphate value calculated by the sensor then a space then the unit designator default uM Collected data shows to 3 decimal places for uM and 4 for mg L and mgP L The data value may also be nan inf or inf then the designator VAP04 The variable a based phosphate calculated by the sensor then a space then the unit designator The data format is the same as CAP04 VAS The variable a value to 3 decimal places Used to calculate VAP04 State The last step in the sample sequence Refer to Table 11 Flush1 The mean value of the last 10 transmittance signal counts in the ambient flush Step 1 part of a sample seq
24. the mean raw count values of the transmittance data values at the end of the initial flush period in the sample sequence The sensor uses a ratio of light through pure sample to light that passes through the sample with reagent to measure ambient water If there is not enough light to move through the pure sample the detector response decreases Phosphate is calculated but at a lower precision Loss of light can be due to stained or fouled windows or data with high absorption scattering in the analytical wavelength 880 nm At 720 counts approximately 20 of the full intensity gets to the detector Good 720 counts and more Suspect 70 720 counts Bad below 170 counts Mixing Spike and Calibration Spike A spike while the sensor does the mix or calibration operation is an indication that there are bubbles in the tubing The height of the spike is frequently related to the level of interference from bubbles The user may change this value if for example the mixing spike data agrees with grab samples to a maximum of approximately 200 counts Good less than 30 counts Suspect 30 100 counts Bad above 100 counts Make an analysis of mixing and calibration data Example of mixing and calibration flags from data collected in file 1 gt MAYOR 300 Spike Mix minimum value Counts 500 Sample Mix minimum value 3 000 Ambient Mix 2 Spike Ambient Output 3 100 end value end value Reference
25. to a non volatile flash memory This command automatically executes when a low input power fault happens before the sensor enters a low power state or when the user exits to PicoDOS Argument 1 None Response The message written on a complete command SUD Start time and date Description Get set the time and date for the first or next data collection sample or for a scheduled pump prime cycle No arguments returns the date and time for the next scheduled data collection sample or none If NOS is 0 it will automatically be set to 1 Argument 1 The start date as m d y optional or P to show the date and time or none of the scheduled pump prime cycle Argument 2 The start time h m s or 0 to stop data collection Argument 3 P to apply the preceding time or date and time to the pump prime cycle start time Response The current or new mode of operation UPC UPS Count Description Get the number of UPS cycles 42 Reference Argument 1 None Response The number of UPS cycles that have happened from low power faults WKM External wake mode Description Get set the operation when an external wake signal happens on pin 1 The default O brings the sensor out of a low power mode Mode 1 starts a data collection sample sequence Mode 2 sensor to show the most recent data collected Argument 1 The wake up mode Response The new or current wake mode 0 off 1 start on wake signal 2 show the most rec
26. 3 for version 1 3 cccccccc 8 character manufacturer identification For example WET LABS mmmmmm 6 character sensor model For example _CYCLE 50 Reference vvv 3 character sensor version For example PO4 _ssssss up to 6 character serial number field Includes leading space _fffffff up to 7 character firmware version field Includes leading space Example address 0 serial number 104 The firmware version is 1 03sd SDI recorder sends Ol sensor sends 013WET LABS CYCLEPO4___104_1 03sd lt CR gt lt LF gt Start Measurement aM Start Measurement and Request CRC aMC Response attin lt CR gt lt LF gt Purpose starts a measurement Notes a address 0 9 ttt measurement time in seconds The sensor always sends 000 n 7 The number of measurement values the sensor makes and returns after subsequent Send Data commands If a scheduled prime sequence is pending or in operation the sensor will ignore the Start Measurement and Start Measurement and Request CRC commands The sensor needs approximately 1800 seconds 30 minutes to complete a measurement The SDI 12 specification allows measurement times from 000 to 999 The sensor uses a save and forward technique to work around this limitation The sensor always sends the measurement time as 000 seconds Measurements are saved in first in first out FIFO memory If the SDI rec
27. A A A Senet A ES 63 4 3 13 1 Sample collection schedule ooooconnccnndnnncnnnnnninncccoccnnncrncrrnnr nr 63 4 3 13 2 Cycle deployment calculator oocoiiionnaiancci it dia 64 4 313 37 PUMP CONTOS usos td 64 4 3 1394 SDI CoN roS ooo clic coins ccivacativs fois seuivadecsasediaacesvieeeleceustsevius caieaectvag idas 64 ASAA Monitor TAD nido aa a od adh lia tras 65 43 AS FOSA EA 65 BS 16 OC o A A pdate A Ae eA Ea TAAA eaa EEE 66 4 3 16 1 Cycle QC example iii ciar derinine 67 Section 5 General informati0N oooooooocconccoincconccccoconoocconoconnnconnncon ccoo nnnnn ccoo nn rnnnnnnnn nan nnnnn nn nn enrere 71 NW ATA dd ado dista 71 5 2 Service ANd SUP OTE a a cece r a a raaa p aara are Te A AAO a aa E aAa aa a E 71 5 3 Waste electrical and electronic equipment sssssssssessseesreetrerrttrtrerttrrtrrrttttttrtrttrttrtrtttttrrrtrrttnnt 71 Section1 Specifications 1 1 Mechanical Rated depth 200 m Weight in air 6 8 kg w reagents Weight in water 0 37 kg Height w handle 56 cm Diameter 18 cm Temperature range operation 0 35 C 1 1 1 Bulkhead connectors 1 1 1 1 6 contact connector Contact Function MCBH 6 MP 1 Ground RS232 RX External wake Voltage in RS232 TX OJJ AJOJN Analog 1 1 1 1 2 8 contact connector Contact Function MCBH 8 MP 1 Voltage in Ground No connect Bi directional SDI 12 RS232 TX
28. A SEA BIRD COASTAL 150817CyclePO4 Cycle phosphate PO sensor User manual 08 2015 Edition 6 Table of Contents Section T IPECHICAMONS ii AAA E 3 A A ates ouaGguarenctetecsaticisameeet see 3 1 11 Bulkhead COnneClOlrs iiie iiia E EE AEO E EE ERRE 3 1 1 1 1 G CONtact connector in eiae e aea aa daia da aaa T aeai aa aa a Eear Ean Ena 3 1 1 1 2 B CONtact CONNECtOT sre a e a a EA A EEA EE EA EAA EAA 3 1 27 Electrical eyiini a a a a a e a E 3 o RO 4 A A 4 Section 2 Operation tia ad 5 2 11 SENSOR SCD Bsn RGA Antes Bie AE RRA EERE oie caso ala ad AREE ohh en 5 2 1 1 Assemble the SenSOF ccoconcccncccncccnccnnccnnccnncnnn cnn rr rre 5 2 1 2 Install the Software ooocccccccnccnnnnnnccnncnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnennnnnnnnnnnnnnnnnnnrnnenanenenenaninininnsS 8 2 1 3 Pime the SONS A A AA ati 9 2 1 3 1 Prepare to prime the SCMSOM cceccceeeeeeeeee cece teee eect anna nn nn nn nn nnnn rn nn 9 2 1 3 2 Prime sensor With VACUUM ssteissi en eie oe D Ta aaeain 11 2 1 3 3 Fil sensot TONS p i p re ar EE aA ap Aa ERES dia 11 2 2 Prepare sensor for deployMeNt ooooooococcccconcconononnnonnnnnononnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnennss 12 O 15 2 31 Modest operation ea ea E ia AE REE AREER aE AEAEE EEEa a EE EREE 15 2 3 2 Set up for deployMeNtcoccoocccocccocococonononoonno nono nono eee cnn nro nannan nannan naene nenene 15 2 32 OD OPM A A A E E E 16 PAS EAN stad a N Gasad le a tis otelaadaleeten
29. Files 1 000 Properties 1 100 Save as 1 200 Print 1 300 Zoom In gt a ZoomOut gt a 2 1 400 Both Axes a P O 1 500 Domain Axis Range Axis 1 600 Select Domain Axis to see the time e Select Range Axis to see the phosphate concentration 4 3 12 1 PO plot tab Look at the data as the concentration of phosphate in selected units Refer to Table 10 on page 56 in the Output formats section for more information 61 Reference Cycle Host s n 000 Run 1042 0 784 pM at 05 02 09 13 38 36 Time and Date Settings Status Ide Get Settings POT MMIDDPYY HH MM SS Time to next sample Time to Prime Host 10 22 09 09 27 00 Rate 19200 vw Apply New Settings a Cyde 10 22 09 09 26 59 Run Skep Casos Len C Stop after next current sample Host Computer Offload Dir C CydeDatalRun1 Status PO4 Plot Raw Plot Settings Monitor Files Po4 356 356 9 ay ani Date MM dd yy HHimm ss PO4 The user can also look at stored data in this window Refer to Replay Selected Files in the Files tab 4 3 12 1 1 PO plot controls Open the plot controls part of the PO Plot tab to look at PO data and whether it is in the range selected Go the to the Tools menu then select Options then Max PO4 Plot Threshold uM to enter this value Other controls the user can select Look at and clear data points in the plot area Look at out of range data Look at
30. LF gt Notes stops the current measurement then turns the pumps on to flush the sensor The sensor flush takes approximately 5 minutes If aX_STOP is sent while the sensor is not taking a measurement the sensor does nothing and no flush sequence is started aX_STOP Example address 0 a measurement is being taken SDI recorder sends OX_STOP sensor sends OSTOPPED lt CR gt lt LF gt the measurement stops and a flush sequence starts Stop Measurement aX_CLEAR Response aCLEARED lt CR gt lt LF gt Notes starts the SDI system All data in the FIFO buffers are cleared Any scheduled prime sequences are cleared If aX_CLEAR is sent while the sensor is taking a measurement the sensor stops and a flush sequence starts aX_CLEAR Example address 0 a measurement is being taken There is already one set of measurement data in the FIFO buffer SDI recorder sends OX_CLEAR sensor sends OCLEARED lt CR gt lt LF gt the FIFO buffer is cleared The measurement stops and a flush sequence starts Prime aX_PRIME Response PRIME lt CR gt lt LF gt Notes sends PRIME Y lt CR gt lt LF gt and starts a prime sequence If a measurement is currently under way the prime sequence will not start correctly Use the Get Prime Status command to verify that a prime sequence started correctly aX_PRIME Example address 0 a measurement is not being
31. SDI 12 mode choose the synchronous slave mode in the Deployment Wizard b All other modes connect the sensor to a battery pack or other power supply 8 Push Next 9 Complete the steps in the Deployment Wizard a Choose the prime and sample start times that give sufficient time to deploy the mooring b Push Finish c Push Yes to put the sensor into a low power mode 10 Make sure that the sensor is in a low power mode 11 Disconnect the sensor from the test cable and PC 12 Fill the filters with DI water Make sure there is no air in the sensor refer to Prepare sensor for deployment on page 12 for details 13 If possible keep the sensor in a bucket in approximately 20 cm of water while the sensor travels to the deployment site 14 Put the bucket of water with the sensor in it in the water at the deployment site This will keep air from getting into the sensor 15 Make sure that the waste tubing on the top of the sensor has no blockages or kinks 2 3 4 Complete the deployment It is important to make sure that the sensor does not get air bubbles inside it when it is removed from a deployment Stop data collection before the sensor is pulled from the water and before power is supplied again If data collection is not stopped before power is supplied to the sensor again it can start operation and pull air in Also do not let the reagent cartridges become empty or the pumps can make air bubbles The manufacturer recommends t
32. age 36 for details on cleaning the red cartridge 3 Make sure the cartridges are installed on the sensor 4 Wind the outlet tubing around the eye bolts 3 9 2 Long term storage The waste solution from the sensor cartridges is Hazardous Waste Follow the applicable regulations to discard the solution Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses Make sure that the sensor is clean and has been flushed before it is put into storage for as long as several months 1 Clean any biofouling from the protective sleeve 2 Clean and flush the sensor Refer to the steps in Send reagent cartridges back to manufacturer on page 36 for details on cleaning the red cartridge 3 Use the syringe to fill the cartridge with DI water 4 Attach the Tygon tubing to R2 5 Turn the sensor on 6 Atthe Settings tab type 200 in the number of pumps area 7 Push Run Pump s 8 Fill and flush each cartridge Keep flow passages filled with DI water 9 Wrap the outlet tubing around the eye bolts 10 Keep the reagent cartridges in a refrigerator Maintenance 38 11 Replace any worn parts 12 Lubricate the bulkhead connectors 13 Attach the protective dummy plugs and lock collars 14 Attach the protective sleeve to the sensor 15 Put the sensor in its case for safe storage Section4 Reference 4 1 Description of nutrient units Nu
33. attach the protective cover to the sensor Figure 17 Protective cover removed 2 Support the bottom of the sensor and lift into a vertical position 3 Pull the sleeve up and off the sensor Keep the sleeve and the screws 3 1 Clean and lubricate bulkhead connector Lubricate the contacts of bulkhead connectors at regular intervals with pure silicone spray only Allow the contacts to dry before they are connected Make sure that the pins have no corrosion which looks green and dull Make sure that the rubber seals on the pins are not delaminated Connectors should connect smoothly and not feel gritty or too resistant The manufacturer recommends 3M Silicone Lubricant spray UPC 021200 85822 Other silicone sprays may contain hydrocarbon solvents that damage rubber DO NOT use silicone grease DO NOT use WD 40 The wrong lubricant will cause failure of the bulkhead connector and the sensor 3 2 Clean macro fouling Wash and scrape clean any macro fouling from the sensor to keep it in good condition Do not wash with a pressure washer Remove any anti fouling tape before the sensor is returned for servicing 3 3 Clean sensor flow paths with cleaning solution Do not operate the sensor with Micro 90 in it It can damage the sensor 31 Maintenance Make sure all of the Micro 90 is flushed out of the sensor Clean the flow paths between each deployment with a 2 cleaning solution of Micro 90 or Liqui Nox
34. cen etna ea a 17 2 3 3 Deployment Procedures orige a eee nro cnn nro 18 2 3 4 Complete the deployMent oococccnninnccnnccnnconnconnnenincnnnonnncnnnrnnn rn n creer 18 2 4 Optional in laboratory performance analysis ooonccnonccccnnncccnonanannncnnnncnnnnnnn nn ncnnnnn nana nrrrcnnnnnnn 19 2 4 1 Setup for in laboratory performance analySiS ooooniccnonccccnnncccnononcnnnnnnnncnonnnnnnnnnnnnnnanannnnns 19 2 4 2 In laboratory sensor stability analysis 0 0 eect teeter reece eee eee e terete aeeeeeeeeeeeeeeenaaae 20 2 4 2 1 Use of water tanks for in laboratory performance analySiS e cceeeeeeeeeeeeeteee 22 2 4 3 NIST check standards for in laboratory performance analysis oooooooccccnnnnnccnccccccccccccccnnnnns 22 2 4 4 Solutions for in laboratory performance analysis oooooccocccccccccccccnnnonononcccncncnanannnnccnnnnnnnns 22 ES AE O 22 O A RE re ET eC ee NT OEE Ce e ee ee eer 22 2 5 2 Operation SEQUENCE ccccccccccccccecececeeeceeeceeeceeeccecceeccneccnecceeeceeceseccueccecececeeeseeeseseeseeeeaess 24 2 5 3 BIANKFUN CXAMPIG ii A ia ia a aaa 24 2 5 4 Good Quality calibration data cienczinesaisiditaneslnceaanseg naenedeniaiacice pa rai n 25 29 5 Bad di ada 26 20 Data QAO C tactica isis rasti 26 2 6 1 Open data files for analysis lt ciromiiaa cee uyecceancste davbvauadss acteneeeivns laedontadeetasy eee 26 20 2 Compare data Miles oi A ADS 27 Section 3 Maintenance escoria atadas 31 3 1 Clean and lub
35. checksum of eight hexidecimal characters Data being collected will stop while the file is moved If GET is used the data output stream will have pauses for the sensor s internal requirements REN Rename Description Change the name of a specified file on the flash drive Argument 1 The path and the file to change Argument 2 The new path and the file name Response lt CR gt lt LF gt Not found error the file does not exist Rename error the file exists or there was another error RMD Remove Directory Description Erase the specified sub directory if it is empty Note that an open subdirectory cannot be erased even if itis empty Use the DSD command to change the data sub directory first 46 Reference Argument 1 Path to the empty directory to erase Response Dir Removed directory is erased error code directory does not exist Dir not empty error directory is not empty TYPE Type Description Shows the specified files from the serial port There is no data verification or way to see the end of a file Wildcards and are permitted Argument 1 The files to show Response Directory then filename separated by a character then lt CR gt lt LF gt 4 2 4 Miscellaneous commands DAC Set DAC output Description Set the level of output of the Digital to Analog converter to a specified l
36. d is completed Reference DSI Device Specific Information Description Get device specific pump volumes the optical pathlength the PO offset and the scale factor Argument 1 The ambient sample pump volume in pL Argument 2 The pump volume of the calibration standard in uL Argument 3 The pump volume of reagent 1 in uL Argument 4 The pump volume of reagent 2 in pL Argument 5 The PO offset volume in uM Argument 6 The calibration offset in uL Argument 7 The cell pathlength in cm Response The current value The arguments in this table show in order from argument 1 to argument 7 separated by space characters 0x20 The pump volumes and pathlength show to two decimal places The offsets show to four decimal places EUF Engineering Units Format Description Get set the units to use for engineering units output Argument 1 The units to use 0 microMolar uM 1 milligrams of phosphate per liter mg L 2 mg of atoms of phosphorus per liter measured in the form of reactive phosphate mgP L Response The new or current values as UM mg L or mgP L IDT IDle Timeout Description Get set the communication idle time in seconds If no communication is received within this time period while a data sample is not running the sensor goes back to a low power sleep state Argument 1 The new communication idle timeout in seconds
37. dges The second is a ruggedized plastic case with the sensor and spare parts 1 Remove the sensor and the spare parts kit from the plastic case 2 Remove the cartridges from the fiberboard box 3 Set the sensor on its side to remove the seven screws that attach the protective sleeve to the sensor 4 Remove the seven Phillips screws on the sleeve Keep the screws Figure 1 Screws removed from protective sleeve 5 Hold the bottom of the sensor and bring it vertical to slide the protective sleeve off Figure 2 Sensor with protective sleeve removed 6 Look at the sensor One side has the intake tubing to the sample port marked with S and calibration tubing The other side has reagent tubing Operation Figure 3 Intake tubing Figure 4 Reagent tubing Cartridge color Location on sensor Contents Blue S phosphate Yellow R1 ascorbic acid Red R2 sulfuric acid lt 10 7 Remove the reagent cartridges from the box and unwrap each cartridge Note that the cartridges are indexed so each one will only fit in one place on the sensor 8 Install the blue calibration cartridge first Hold it above the upper housing above the intake ports Operation Figure 5 Blue calibration cartridge installed 9 Set the cartridge on the guide pins and push down until it clicks into place If the cartridge does not click into place lift it off of the guide pins and push on the stai
38. e and flush the sensor or stop immediately If the flush occurs NOS goes down as though a whole sample was completed If a sample is not being collected the SUD and SUD P move to 0 and the NOS value does not change Argument 1 no argument the flush sequence occurs the sample sequence stops The NOS does not change as though the sample sequence did not occur Response Stopped shows if there was no sample sequence Flushing shows if the user made the command without the I argument Stopping shows if the user made the command with the l argument TPC Total Pump Counts Description Get the total number of pump actions by any or all of the pumps in the sensor The pump counter is reset when a new pump is installed Argument 1 none Response Shows the counts for the ambient sample pump the calibration pump blue the reagent 1 pump yellow and the reagent 2 pump red separated by spaces These are the total counts over the lifetime of the pumps installed by the manufacturer TTS Time To Next Sample Start Description Get the time until the start of the next scheduled sample sequence Argument 1 none Response Shows the time in h mm ss until the next sample starts Shows a 0 if a sample is being collected or 1 if a sample is stopped VOL Volumes Pumped Deployment Pump Counts Description Get set the volumes for all the pumps in the sensor for this deployment An optional argument is R S resets all four pump volumes to 0 00 Argu
39. ed by the sensor for the R1 reagent pump volume in pL r2v rr rr The pump volume saved by the sensor for the R2 reagent pump volume in pL ZO Z ZZZZ The zero offset saved by the sensor in uM CO 0 0000 The calibration pump volume offset saved by the sensor in pL Cas Ss ss The constant a value saved by the sensor st t ttt The slope threshold which is usually negative saved by the sensor 57 Reference Table 13 Raw file first header line output continued Field Description cc 10 53 The calibration standard concentration saved by the sensor ups u The UPS counter saved by the sensor It increases in increments of one each time the power is removed from the sensor when the sensor is not in a low power mode flt ffff A four character hexadecimal code that shows fault conditions within the sensor A value of 0000 shows that no faults are detected 4 3 5 File and tools menus This is the start of your topic Table 14 File menu commands Item Shortcut Exit Ctri Q Description Exits the software Explore Offload Dir Ctrl E Opens the directory of files that shows the files in the Host Computer Offload Dir area as Windows Explorer files that can be opened with a text editor Load File s Ctrl O Lets the user select the data files to compare in the PO 4QC tab and in the Analysis Plot window Save Analysis Set Ctrl S Saves a user named summary file of the most curre
40. enter Alt O to open the Cycle Host Options window The user can look at and change the values in this window Push Reset to Defaults to return to the manufacturer set values Operation Low Sig 170 750 col 00 Out Of 0 05 0 075 10 5 40 p Mixing 00 00 30 100 Cal Spike 00 00 30 100 The ranges of values for Good Suspect or Bad are given in the table below Value output from sensor Flag Value lt Min Sus Bad Min Sus lt Value lt Min Good Suspect Min Good lt Value lt Max Good Good Max Good lt Value lt Max Suspect Suspect Value gt Max Sus Bad 29 Operation 30 Section 3 Maintenance Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses PPE includes a laboratory smock safety glasses and gloves Replace the Cycle reagent cartridges and intake filters approximately every 1000 samples and clean the optics flow path Change the battery core of the battery pack if that is the power source for the Cycle The sensor is calibrated to output a reactive phosphate concentration in user defined units of UM mg L or mgP L The sensor will operate for approximately ten 1000 sample deployments between service and re calibration by the manufacturer 1 Put the sensor on its side and use a Phillips screwdriver to remove the seven screws that
41. ently completed sample GLSO on the wake signal 4 2 2 Operation commands FLT Fault Status Description Show any fault conditions Argument 1 none Response A four character hexidecimal value that shows the fault status The value 0000 shows that there are no faults Table 4 Fault conditions Bit field Fault 0x0001 Invalid values at startup The default values are used 0x0002 The values used at startup were not saved Some values may not be in effect 0x0004 Invalid operational values at startup The default values are used 0x0008 The operational values used at startup were not saved Some values may not be in effect 0x0010 The saved data subdirectory did not exist at startup and could not be made 0x0020 Communication to the gas gauge controller was not made 0x0040 SYS QPBCS is not set correctly GLSO Get Last Sample Output Description Get the data for the most recently completed or ended sequence of data collected Argument 1 none Response The most recently completed or ended sample collection sequence followed by two pair of lt CR gt lt LF gt characters Refer to Error Reference source not found for the data format KCO Description Operates a user specified number of cycles to flush the sensor after the reaction is complete and the analytical signal is selected Argument 1 Positive integer for cycles at 2 Hz Response The
42. er that shows the current step in the sample sequence Refer to the Step values on page 56 code A legacy code number that shows which pumps are in operation counts An increment counter that shows the number of transmittance measurements left in the current step of the sample sequence vin The voltage of the power supply to the sensor to two decimal places 4 3 3 Summary file format The summary files have information for all the samples in each of the sensor s sub directories Pate Time Run CAPO4 VAPO4 VAS State Flush1 Amb Min Flush2 Cal Min Remaining Diagl Diag2 After the header line there are one or more lines of data collected This ends with a lt LF gt 0x0a character Refer to the Engineering units output on page 56 for details 4 3 4 Raw file format The raw sample text files have two header lines that come before the raw transmittance measurements then one or more lines after the raw transmittance measurements The format is shown below Ver V VV av a aa cv cc cc riv yy yy r2v rr rr ZO Z ZZZzZz CO 0 0000 cas ss ss st t ttt cc 10 53 ups u fl1t ffff lt 1f gt Table 13 Raw file first header line output Field Description ver V VV The firmware revision number that the file was collected under av a aa The pump volume saved by the sensor for the ambient pump volume in pL CV CC CC The pump volume saved by the sensor for the calibration pump volume in pL rv yy yy The pump volume sav
43. es yes 7 yes yes yes no 15 yes yes yes yes VIN Input Voltage Description Get the input voltage to the sensor in volts Argument 1 none Response The input voltage to the sensor shows to two decimal places It shows approximately 0 2 V lower than the voltage supplied to the sensor VSN Version Description Get the firmware version in the sensor Argument 1 none Response The firmware version n nn where n is a digit from 0 9 4 2 5 SDI commands The sensor supports all basic SDI 12 commands Refer to the SDI 12 specification at www sdi 12 org for details of the command protocol The SDI 12 has been tested with the SDI 12 Verifier from NR Systems Inc http www sdi 12 verifier com with up to 1000 ft of 24 gauge cable for communication NOT power For any command not described below the sensor will respond according to the SDI 12 v1 3 specification Definitions e a is the sensor s SDI 12 address e lt CRC gt is the 3 character Cyclic Redundancy Check e lt CR gt is a Carriage Return character e lt LF gt is a Line Feed character Table 7 SDI data formats Index Description Format 1 Sample date YY MMDD 2 Sample time HH MMSS 3 Run number dddd 4 PO concentration d dddd OR dd ddd Note that 99999 shows as the PO concentration index 4 if an unrealistic PO value is determined a nan in the summary file This happens when a sequence is stopped early or the calculation gives an unde
44. ess than the number of the next sample Response The current or new sample counter CSF Cal Spike Frequency Description Get set the frequency that a cal spike is done The default is 6 Argument 1 The new frequency The next data sample after this command will do a cal sequence Allowed values are 1 to 32767 If a data sample is being collected the new value will not change it Response The current or new cal frequency followed by the number of data samples before the next cal spike The two values are separated by a space character 0x20 A value of zero for the number of data samples before the next cal spike means that the next sample will run a cal spike DCA Deployment Cartridge Amounts Description Get set the quantity of chemicals in the sensor at the start of a deployment Argument 1 The calibration standard in mL Argument 2 The quantity of reagent 1 in mL Argument 3 The quantity of reagent 2 in mL Argument 4 S safety flag Response The current or new cartridge quantities to three decimals in milliliters at the start of a deployment The values are separated by a space character 0x20 DSD Data SubDirectory Description Get set the subdirectory used to save data Argument 1 The name of the subdirectory without the root directory e g C Only subdirectories of the root directory are allowed Subdirectory names can be no longer than 8 characters Only a z and 0 9 are permitted Response The message resets when the comman
45. et approximately 2 cm of air into the tubing b Put the inlet tubing back in the beaker Let approximately 2 cm of water into the tubing c Do the two steps above until the inlet tubing is filled with 2 cm sections of air and water Put the end of the outflow tubing in the beaker again Attach a syringe with a Luer lock to a 1 16 hose barb and then the outflow tube on the sensor Pull the plunger to the 15 mL mark to fill the syringe Push Flush if necessary Remove the syringe and put the inlet tubing in the waste beaker Push Flush two more times to make sure the sensor has been flushed three times The sensor is now clean Disconnect the syringe and tubing and the inlet tubing from the S port 3 5 Replace reagent cartridges Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses 1 2 Remove the blue calibration cartridge first Press the stainless steel coupler on the bottom of the cartridge to release it Slide the cartridge up and off the guide pins 3 Pull the cartridge away from the housing and set the cartridge aside Figure 22 Blue cartridge removed 34 4 5 Maintenance Remove the yellow and then the red cartridges Install new cartridges Refer to the section on Assemble the sensor on page 5 for details on this procedure 3 6 Replace intake filter and screen O o NCD 0 A oN Disconnect the tubing
46. evel Argument 1 The 12 bit value shows in hexidecimal format from O fff Response The new DAC level of output Table 5 Common DAC output voltages Value Approximate output voltage 0 0 00 333 1 00 400 1 25 666 2 0 800 2 5 999 3 0 c00 3 75 ccc 4 00 fff 5 00 DEV Device Type Description Get the device type and serial number of the sensor Argument 1 none Response The device type and the serial number of the sensor separated by a space PO4 device type nnn three characters where n is a number fro 0 9 H HELP Help Description Show the available commands Argument 1 none Response See table below Table 6 Summary of Commands Type of command C Configuration O Operation F File M Miscellaneous 47 Reference Type of Command Description Type of Command Description command command C CAS const a C CCS calibration concentration C CLK get set date and time C SCNT sample counter F CPY source destination C CSF calibration frequency M DAC set dac output C DAT get set date and time C DCA get set cartridge volumes F DCS default config F DEL file M DEV get device type F DIR D for data dir C DSD data subdir C DSI device config C EUF get set units format O FLT get fault status F GET get file s O GLSO get last output C IDT idle timeout C SINT sample interval O KCO operate pumps to flu
47. fined result 5 Units of PO concentration d 0 specifies microMolar uM 1 specifies milligrams phosphate per liter mg L 2 specifies mg of atoms phosphorus per liter measured in the form of reactive phosphate mgP L 49 Reference Table 7 SDI data formats continued 6 Last sample status d 0 Initial flush 1 Ambient read 1 2 Mix ambient 3 Ambient PO read 4 Completed normal run 5 Mid flush 2 6 Ambient read 2 7 Mix spiked 8 Spiked PO read 9 Completed spike run 7 battery voltage volts dd d Table 8 SDI Commands Acknowledge Active a Response a lt CR gt lt LF gt Purpose verifies the SDI 12 address Example address 0 SDI recorder sends 0 sensor sends 0 lt CR gt lt LF gt Address query Response a lt CR gt lt LF gt Purpose shows the sensor s SDI 12 address Example address 0 SDI recorder sends sensor sends 0 lt CR gt lt LF gt Change address aAb Response b lt CR gt lt LF gt Purpose changes the sensor s SDI 12 address to b The default address is 0 Example address 0 SDI recorder sends 0A1 sensor sends 1 lt CR gt lt LF gt address now 1 Send identification al a capital Response allccccccccmmmmmmvwv_ssssss_fffffff lt CR gt lt LF gt a sensor address Il lowercase L 2 character SDI 12 version For example 1
48. gerous gasses Obey the safe handling and disposal instructions on the bleach containers Do not operate the sensor with bleach in it It can damage the sensor NOTICE Do not pre dilute bleach It will go bad Only use bleach well before the expiration date on the container It may be necessary to clean the optics with a bleach solution if the data output of the sensor does not increase by approximately 2500 counts after it is cleaned with the Micro 90 solution Table 3 Bleach dilutions Description Dilution Clorox Pro Results concentrated outdoor 9 1 Clorox Ultra 2 1 Regular none A 9 1 dilution is 9 parts water to 1 part bleach For a 40 mL beaker the solution is 4 mL of outdoor bleach 36 mL of water 3 o Maintenance Make sure that the sensor has been flushed with clean water Do the procedures in the section on Clean sensor flow paths with cleaning solution on page 31 but use bleach not Micro 90 When the bleach flush procedure is complete make sure to thoroughly rinse the flow paths with clean water 3 4 Flush cleaning solution from flow paths 9 Rinse the inlet tubing or get a new length to connect to the S inlet Fill a clean beaker with approximately 100 mL of clean water and put the other end of the tubing in the beaker Go to the Settings tab of the Cycle software and push Flush a Pull the inlet tubing out of the beaker of water L
49. h Finish then Yes to make a results report The results report records the new values and the previous values Cycle Deployment Wizard oo Awake PO4 gt UKM D PO4 gt WKM O D PO4 gt CLK 04 17 09 PO4 gt CLK 04 04 17 09 PO4 gt VOL 11 875 2 190 27803 so PO4 gt VOL 0 00 0 00 0 00 0 00 S 0 000 0 000 0 000 0 000 PO4 gt CNT The sensor goes into a low power mode Disconnect the sensor from the power supply 2 3 Deployment Operation 2 3 1 Modes of operation There are six modes of operation Both raw and engineering units for each sample are stored in the sensor s memory Cycle modes of operation Host controlled mode The sensor is connected to a host PC and is controlled and monitored by the Cycle software The user can look at the sensor s data output and other status indicators in this mode Autonomous mode The sensor operates by itself for example installed on a mooring with a battery pack to supply power Deployment the sensor is installed on a mooring that has no controller or data logger The power is supplied by a battery pack Asynchronous slave mode The sensor is connected to a master controller At certain intervals the controller pulls the most recent data from the sensor The sensor collects data on its pre determined schedule independent of the controller The controller supplies power to the sensor Deployment the sensor is installed on a mooring with a system controlle
50. hat the user retrieve the sensor in a bucket that has approximately 20 cm of water in it so that the sensor stays submerged for travel 1 For the SDI mode of operation stop the SDI recorder 18 Operation This will stop the SDI but not the sensor Turn the power off to the sensor and then back on to stop any active samples The sensor will save the last sample even when the power is turned off For SDI mode of operation Send an aR command The sensor will send the data values from the previous sample Disconnect the cable from the sensor D e Remove the sensor from the mooring Connect the sensor to the host PC and power supply with the test cable Turn on the sensor Push Refresh Directory Listing under the Files tab and save the summary txt file and any other desired files from the current data sub directory o N EE 2 4 Optional in laboratory performance analysis The waste solution from the sensor cartridges is Hazardous Waste Follow the applicable regulations to discard the solution Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses To make an analysis of the sensor s performance make sure the sensor is primed and that the collected data is accurate and stable Operate the sensor overnight to make sure the collected data is stable Make sure the sensor does not run out of solution to sample or it will pull in
51. he sensor must have a minimum of 10 5 VDC at 2 amps The decrease in voltage over 30 m of 18 gauge cable is approximately 2 2 V Use a standalone power supply if the SDI recorder cannot supply 2 amps Connect the negative terminal of a standalone power supply to the ground terminal of the recorder Do not connect the positive terminal of a standalone power supply to any terminal on the recorder Make sure to add the power requirements of any SDI capable sensor to the total current requirement Sample setup 1 Equipment Power requirement sensor Cycle PO 10 5 VDC 2 amps cable length 60 m 200 ft 4 4 VDC 200 x 2 2 cable gauge 18 power supply SDI recorder that supplies 12 VDC at 0 5 amps 14 9 VDC at 2 amps Sample setup 2 Equipment Power requirement sensor Cycle PO 10 5 VDC 2 amps sensor SUNA 12 18 VDC 1 amp cable length 30 m 100 ft 3 3 VDC cable gauge 18 power supply SDI recorder that supplies 12 VDC at 0 5 amps 15 3 VDC at 3 amps Note Set the Cycle PO and SUNA to different SDI addresses Change the Cycle from its default of 0 to 1 before deployment 2 3 2 2 SDI deployment Connect the SDI cable to the 8 pin connector on the sensor 2 Connect the other end of the SDI cable to the SDI recorder and a 12V power source Note that the power supply must supply a minimum of 2 amps 3 Configure the SDI recorder to send aM or aC commands at the chosen
52. ibration pump to operate for 100 pump cycles 35 Maintenance Pump Controls Et Ss Num Pumps 100 Run Pump s Stop Push Run Pump s While the pump is in operation pull a light vacuum approximately 1 5 10 mL of the full travel of the plunger After the pump has operated for 100 cycles make sure that the reagent tubing that connects the cartridges and the inlet barbs does not have any air bubbles Look at the tubing from the reagent cartridges to the manifold to check for bubbles If bubbles are present do steps 4 and 5 again If bubbles are small it may not be possible to remove them Do steps 3 5 with R1 and R2 Disconnect the tubing from the syringe Put the end of the tubing into an empty receptacle Go to the Settings tab and push Flush The Sample fluid opening is primed 3 8 Send reagent cartridges back to manufacturer Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses Return Policy The manufacturer will recycle cartridges sent back by the user The manufacturer will only accept red reagent cartridges that have been drained and flushed Do the steps below to prepare the red cartridge to send back to the manufacturer 1 2 Disconnect the red cartridge from the sensor if necessary Make sure the cartridge is unlocked Figure 24 Fluid coupler lock 36 Push the stainless steel tab i
53. id commands will show Bad command or file name Invalid parameters or arguments will show invalid argument s A command that cannot be accepted while the sensor is collecting a sample will show Not available while sample running 39 Reference 4 2 1 Configuration commands 40 CAS Constant A Star value manufacturer s scale factor Description Get the constant a value Argument 1 none Response The constant a value to two decimal places CLK or TIME Get set the sensor s internal clock or date Description Get set the sensor s internal clock or date Argument 1 The new time in hh mm ss 24 hour clock or the new date mm dd yy if both the date and time are set Argument 2 The new time in hh mm ss 24 hour clock if both the date and time are being set Response The current or new date and time in mm dd yy hh mm ss format The hours are in a 24 hour format The date and time are separated by a tab character 0x09 not by a space CNT Sample CouNTer Description Get set the current or most recent sample number This is the number used in the raw file naming format When the sample counter is set the next sample run is the newly set count plus 1 Set this value to 0 at the start of each deployment Argument 1 New count value for the number of data samples completed Must be followed by a S switch to change the count Set to one l
54. indow to another program for evaluation HB Cycie Host 5 n 000 Run 89 0 577 pM at 08 05 09 09 05 12 fie Took Hep COMM Settings Tree ond Date Settings Stu Sepe com 6 X Get Sets POT MMICON NY tSS Teme to rent sangit eee Mosti 10 22 09 10 00 26 Tera to Prime Rate 19200 e Ach News Settings a gt T Cde 102209100025 an a Sep Osu Dres eee O op after nextiourert sample Host Computer Offload Dr CAER y Rats POA Piot Raw Piot Settings Monitor Fies w Awake sI 25 00 8 00 8 00 8 00 0 0000 0 0000 00 was 2 75 lt 4 3 15 Files tab Use the software to get the data that is saved in the sensor 1 Start the software if necessary 2 Select the Files tab Cycle Host Fie Tools Help COM Settings Time and Date Settings Status Idle com sm Get Settings PST MM DD YY HH MM S5 Time to next sample Unknown 4 Host 01 04 12 15 04 00 Time to Prime Unknown SEES Apply New Settings ea Clear Changes Stop after next current sample Host Computer Offload Dir C CycleData EJ pee Status PO4 Plot Raw Plot Settings Monitor Files Cycle Files IO Instrument Files Cycle On Board Data Storage Dir E RUNI RUNZ e 00000004 TXT On Board Disk Storage 00000005 TT Free Space 950 304 768 00000002 TXT SUMMARY TXT Used Space 97 058 816 00000001 TxT Total Space 1 047 363 584 H DATA Refresh Root Directory Listing Offload Selected File s Dir Re
55. lat stable flush periods downward spike during mixing no signs of bubbles and all seven lines agree well Counts R 500 00 30 00 00 35 00 00 00 00 00 05 00 00 10 00 00 15 00 00 20 00 00 Time 25 Operation 2 5 5 Bad data The graph below is an example of bad data caused by bubbles 2 6 Data QA QC Select QA QC File Plot tab to make an analysis of the quality of the data collected by the sensor Use the software to open and look at the data in a number of files There are algorithms in the software that let the user compare elements of the collected data as good bad suspect or not evaluated 2 6 1 Open data files for analysis The user can select a number of data files for analysis Note that the more files that are selected the more time it takes the software to process the data 1 If necessary start the software 2 Select the QC Plot tab 3 Push the three dots to look for the directory with the data to look at Host Computer Offload Dir c aaSBCUserManuals Cycle FORT_02 Ja E Status PO4 Plot Raw Plot Settings Monitor Files PO4 File Plot 4 Go to the File menu then Load File s 5 Select the files to look at Ihokion My Moenen ele Tike ane cco0c553 TXT 09909534 TXT D0290555 77 00300556 TXT GI te eh yw untar Hes 6 Push Open The Analysis Plot window opens and shows the selected graph 26 Operation Counts 1 000 1 250
56. led disabled n n the interface3 board version number c the code that shows the status of operation and either enabled or disabled to show if the board is on or off 0 8 the board is being started or there is a problem 9 the board is OK but does not have power from the SDI bus 10 there is no SDI interface board or it is not in communication 11 the board is OK and has power from the SDI bus SLP Sleep Description Put the sensor in a low power sleep mode for a specified time until a specified time or indefinitely Use the serial port or the external wake signal to put the sensor in a ready mode Some characters are baud rate dependent and cannot be used to wake the sensor The character that is used to wake the sensor is not buffered for input The manufacturer recommends that the user does an RS232 break of at least 500 ms to wake the sensor Argument 1 The number of seconds to be in a low power state No argument stay in low power until the next data sample time occurs Response The message Sleeping lt CR gt lt LF gt shows When argument 1 is used or if the sensor is going to collect a data sample the message for n seconds lt CR gt lt LF gt shows where n is the smaller of the value of the commanded interval or the time to the next data sample 44 Reference STP SToP Description Cancel a sample sequenc
57. low for details about how to use the commands Commands are limited to 160 characters which includes the Command characters are case insensitive Characters are converted to uppercase by the sensor but are shown as they are entered e Commands start with an ASCII character 0x24 and end with an ASCII carriage return lt CR gt character 0x0d e The command designator follows the e Command designators are usually 3 or 4 characters One or more arguments follow the command designator Arguments can be separated by a space 0x20 a tab 0x09 or a comma 0x2c Ifa command does not need an argument a lt CR gt line terminator follows the designator Non printing ASCII characters that occur before the that starts a command are ignored and not shown More than one command can go on a single line if separated by semi colons 0x3b The commands operate until there are more than 160 characters per line or there is an error Use the backspace character 0x08 to remove characters from the end of the command The command interpreter will show the backspace and send a space 0x20 and a backspace 0x08 character to delete the removed character Set up the command interpreter with the SPR command The default is enabled which shows the PO4 gt command prompt when it is ready to accept commands Cycle shows the success or failure of user issued commands and end with lt CR gt lt LF gt characters Inval
58. ment 1 Ambient volume from the pump in mL Argument 2 Calibration standard from the pump in mL Argument 3 Reagent 1 from the pump in mL Argument 4 Reagent 2 from the pump in mL Argument 5 S safety flag Response The current or new volumes in mL to three decimal places in the order above The values are separated by a space XIT EXIT Exit Description Stop the firmware application and go to PicoDOS Scheduled samples are stopped Same as SUD 0 Argument 1 none Response None from the sensor application The PicoDOS start message shows at the current baud rate after several seconds 4 2 3 File commands CPY Copy Description Copy a file to a new filename or location on the flash drive Wildcards and are permitted Note that if a destination file already exists it will be overwritten 45 Reference Argument 1 The file or files to be copied Argument 2 The destination of the copied file or files Argument 3 An optional V command to verify the new file or files Response The names of the source files followed by lt CR gt lt LF gt as they are copied After the last file is copied an additional lt CR gt lt LF gt shows followed by n files s copied where n is the number of files copied The message is padded with spaces so that the number field is always eight characters wide and ends with two lt CR gt lt LF gt DEL Delete Desc
59. minutes before it is deployed Enter the total samples for the deployment in the variable box next to Number of Samples Enter the sample interval in the variable box area next to Sample Interval Keep the default of 6 in the Cal Frequency variable box This is the manufacturer s recommended frequency Push Next A summary of the configuration shows 13 Operation 25 26 27 28 29 30 31 14 Cycle Deployment Wizard Settings Summary Autonomous mode Syne to host clock Reset sample counter Reset pover consumption Data dir to existing dir RUN6 NOT RECOMMENDED Instrument units to uM Setting reagent 1 deployment volume to 250 0 Setting reagent 2 deployment volume to 250 0 Priming to start at 16 15 00 on 11 09 10 11 1 minutes from now Sampling to start at 16 45 00 on 11 09 10 41 1 minutes from now Unlimited number of samples De Sample interval 1 00 00 al Send Settings to Cycle Push Send Settings to Cycle If the user selects a configuration that will have a negative effect on the operation of the sensor a window shows A There are non recommended settings Apply them anyway Push Yes Push Yes at the next window to make a configuration report Configuration values will still be sent to the sensor if the user does not want a report and pushes No Enter a name and location to store the report Use txt or log as the filename extension Push Save Pus
60. n The coupler is unlocked Attach an approximately 15 cm long section of 1 8 ID Tygon tubing to the quick disconnect inline coupling These two parts are in the manufacturer supplied spare parts kit Put the other end of the tubing in an empty beaker Attach the tubing with the quick disconnect coupling to the red reagent cartridge Any fluid in the cartridge will drain into the beaker Maintenance Fill a syringe with DI water and fill the cartridge with the water Disconnect the quick disconnect coupling body from the cartridge Shake the cartridge Connect the quick disconnect coupling to the tubing and the cartridge again and drain the DI water in the cartridge into a beaker 10 Do steps 6 9 two more times 11 Put the empty cartridges into a new box with a minimum of 5 cm of protective material around the cartridges 12 Send all three of the empty cartridges to the manufacturer for credit on new cartridges 13 Follow all local laws and regulations to discard the waste water from the cartridges 5 9 N O 3 9 Sensor storage Always flush out all of the reagents in the sensor Push Flush in the Settings tab to do this procedure 3 9 1 Short term storage Make sure that the sensor is clean and has been flushed before it is put into storage for as long as a month 1 Clean any biofouling from the protective sleeve 2 Clean and flush the sensor Refer to the steps in Send reagent cartridges back to manufacturer on p
61. nless steel tab of the fluid coupler to make sure it is unlocked Figure 6 Blue cartridge on guide pins Figure 7 Fluid coupler at the bottom of each cartridge 10 Install the yellow and then the red cartridge The cartridges are installed correctly if they cannot be lifted off of their bases Operation Figure 8 Cartridges installed 2 1 2 Install the software Install the Cycle Host software from the Software tab on the Cycle Host product page on the manufacturer s web site 1 A 8 9 Make a Cycle folder in C Program Files x86 on Windows Vista Windows 7 or Windows 8 or in C Program Files for earlier versions of Windows Make a CycleHost folder in the Cycle folder from the previous step Go to the downloaded zip file Right click on the CycleHost zip folder and choose Extract All Make sure that the Show extracted files when complete checkbox is selected Select a Destination and Extract Files Files will be extracted to this folder C Program Files x86 CycleHost1_07 Y Show extracted files when complete Extract the files to C Program Files x86 CycleHost If asked for Administrator permission push Continue r Destination Folder Access Denied pS i You ll need to provide administrator permission to copy to this folder CycleHost Date created 1 3 2012 11 01 AM E Continue Skip l Cancel 4 Do this for all current items 2 found The
62. nt QC analysis Table 15 Tools menu commands Item Shortcut Description Deployment Wizard Ctrl D Opens the Deployment Wizard Get Settings Ctrl G Gets the settings that are saved in the sensor View Analysis Plot Ctrl Shift Shows or hides the Analysis Plot window A Options Alt O Opens a new window with user selectable options for QA QC analysis of data Remove Selected Analysis Ctril R Removes a selected sample from the list in the Sample Analysis Plot window The user can also right click to Remove Selected on a file or files in the list Clear Analysis Samples Immediately erases all of the files from the list in the Analysis Plot window The user can also push Clear in the QC Plot tab Restore Instrument Settings Use this only if the settings in the sensor are erased 4 3 6 Communication setup Cycle Host s n 000 File Tools Help COM Settings Set the COM port from the host software 58 Reference e Select the appropriate communication port from the drop down menu or Enter the port number in the variable box Go to the Tools menu then select Options to turn off the auto connect option The manufacturer recommends that the user keep the default baud rate of 19200 The Cycle software looks for available communication ports on the host PC and makes those available to the user The port defaults to None the first time the software is used Thereaf
63. on Open data files for analysis on page 26 for details Look at the summary graph in the QC Plot tab Select one of the dots in the graph to open a data file in the Analysis Plot window 27 Operation 28 IV Show out of range data gt i I Show spike plot eerie eee Clear Save Analysis Set I compress timescale I Show VAPO4 Sample Thu 08 06 15 08 26 00 20 CAPO4 2 063 uM VAPO4 1 553 uM Test Flag Value Bubble Spike Good 217 178 cov Good 0 888 Low Signal Good 2 959 Out Of Range Good 2 063 Mixing Spike Suspect 79 Cal Spike Not Evaluated Look at the Test Flag and Value information In the example above the Mixing Spike shows a Suspect Value of 79 Look at the data in the Analysis Plot window Note the spike in the Mixing period green background Put the cursor in the graph above and to the left of the spike Drag the pointer diagonally down and to the right to zoom in on the spike The spike is from approximately 2960 to 2880 a distance of 79 counts It is shown as Suspect in the Mixing Spike row of the table in the PO4 QC tab 2 860 2 940 2 950 2 960 2 970 Push Auto Range in the Analysis Plot window to change the plot back to the default scale or drag the pointer from the lower right to the upper left the opposite direction from step 6 to return the graph to the default view Go to Tools then Options or
64. ooooooccconccnncnnnnonnnnnnnonnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnininins 47 4 20 OD COMMONS 49 4 3 Software reference ccccscccseccseecceeeaeceaeeeaeeaaeeaaeeaaecaaeenaeeeaeaaeceaeceaensaecaaecaaecaaeceaecaesaeeeeeeeeeeeaaes 56 4341 Engineering units OUT UIs is iio 56 4 3 2 Raw transmittance OUtpDUt oocococccococococonocononnnonnnnononnnonononnno nono nono nnnnnnnnnnnnnnnnnnnnnnnnnnnnonnnnnnnnnnns 57 4 33 SUMMARY file TO Madina dd ia 57 4 3 4 Raw file format oocccccccnncnnnnonnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnininess 57 AO Eile and toolS MEUS e AA ASA As 58 4 3 6 Communication SOtUP ooooocccncinncococonocononnnonnnonnno ono nono nono nono nono nono nnnnnnnnn nn nn nn nn nn nn nn nnnnnnnnnonnnnnnns 58 431 Operation VAlUCS iii A ai ads 59 4 3 8 Date and time SCtUP cece cee cece cece eee e cette nc 59 4 3 9 Modes of OperatiON ooooocccocccococococononconcnonnnn cnn nono nnnnnnnn non nnnnnnn nro nn nn nn EEE nnnnnnn nn nn nn nn nn nnnnnnnnnnnnns 59 4 3 9 1 Operation Options usina A A Aa 60 SS ON 60 439 3 Low power optio Nam eed gaagutaaondueis cdue tab a ae an arpe eaa EEn e A aeai 60 4 3 10 Data file storages iii de 60 43 14 Status di Ad 60 A SA AOI A te SEAE A A E CustieSae 61 43 121 POS POMADA RA AA AAA 61 4 3 12 2 Raw data plot tab oococccccccnncccnccnccnnncnnnneninnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnns 62 A SVS Settings tab A
65. order sends a Start Measurement command and subsequent Send Data command the sensor sends the next set of data in the FIFO If there is no saved data the sensor sends a null response a lt CR gt lt LF gt The data recorder or data processor program needs to handle these values appropriately The sensor has the same response to Start Measurement and Request CRC except that it appends a 3 byte CRC to the data when it responds to the Send Data command Example address 0 SDI recorder sends OM sensor sends 00007 lt CR gt lt LF gt SDI recorder sends OMC sensor sends 00007 lt CR gt lt LF gt Refer to the Send Data command for examples of the commands that come after either the aM or aMC Start Concurrent Measurement aC Start Concurrent Measurement and Request CRC aCC Response atttnn lt CR gt lt LF gt Purpose starts a measurement Notes a address 0 9 ttt measurement time in seconds The sensor always sends 000 51 Reference nn 07 The number of measurements the sensor will make and return after subsequent Send Data commands If a scheduled prime sequence is pending or in operation the sensor will ignore the Start Measurement and Start Measurement and Request CRC commands Start Concurrent Measurement aC Has 70 characters Start Measurement aM Has 35 characters Example address 0 SDI recorder sends OC
66. oyment Wizard Choose a mode of operation for this deployment Autonomous Standalone with no external controller Synchronous Slave Sampling initiated by external controller Asynchronous Slave Cycle sampling on a preset schedule Host Continuous control via a host computer Back Next IM Cancel Select Autonomous Standalone with no external controller Push Next Enter the name of a directory in which to store the collected data Note that the name can have only 8 characters Push Next The Priming and Sampling Start date and Time window appears BB cycle Deployment Wizard Priming Start Date and Time January v January aS Sun Mon Tue Wed Thu i Sun Mon Tue Wed Thu 5 a1 d EJ Eo o mw AZ 0 8 9 10 11 12 ii 18 18 03 17 18 19 24 25 26 04 24 25 26 31 05 31 Prime Start Time 09 30 Sample Start Time 10 00 Skip Prime Cycle Number of Samples 2000 Cal Frequency 6 Sample Interval 1 00 00 Back JE Jd Cancel Set up the sensor to do a prime cycle Either use the Settings tab or the Deployment Wizard before the sensor operates Select the date to deploy on the Sampling Start Date and Time calendar Enter the time to deploy at the Sample Start Time variable box Select the date to deploy on the Priming Start Date and Time calendar Enter the time to deploy minus 30 minutes in the Prime Start Time variable box The sensor will begin the priming cycle 30
67. play Selected Files Cancel Offload Refresh Delete Selected File s Dir s Create New On Board Dir Filter TXT Hide Offloaded Current File Lo 3 Push Refresh Root Directory Listing The files saved in the sensor will show in the Files tab Enter the file directory or folder on the PC to save the data from the sensor or create a new folder Host Computer Offload Dir C CycleData Runt 4 Y IC CycleData Data C JatalRuni Status PO4 Plot Raw Plot 65 Reference 4 3 16 QC Plot tab 5 Push Offload Selected File s Dir to move a copy of the data from the sensor to the PC The user can save only one directory at a time but it is possible to select several files at the same time to save to the PC 6 Monitor the data saved to the PC Look at the Current File area at the bottom of the software window Refer to Compare data files on page 27 in the Operation section for more details about the analysis of data that the user can do in the QC Plot tab Other functions are given in this section Status PO4 Plot Real time Plot Settings Monitor Files QC Plot 2 1 2 0 1 9 1 8 1 7 1 6 15 1 4 13 1 2 1 1 PO4 uM X Axis Scaling 7 Time amp Date an EI Sample Order id Selected Sample Information Sample Thu 08 06 15 01 56 01 7 CAPO4 1 989 uM VAPO4 1 482 uM Test Bubble Spike Suspect cov Good 0 Low Signal Good
68. power mode from a standby mode before power is removed 4 3 10 Data file storage Choose the folder on the host PC in which to store the data collected by the sensor Host Computer Offload Dir Cr CycleDatalRunt iv IC CycleData Data CuCycleDatalRuni Status PO4 Plot Raw Plot Store the data from the sensor on the host PC There are two options Enter the file path Find the folder If this is a new folder another window lets the user make sure of the selection and makes the folder The software stores this file path to use when the next software session starts 4 3 11 Status tab The Status tab shows the estimated volumes of chemicals in each of the sensor s cartridges Push Get Settings or Refresh to get information on the cartridge volumes 60 Reference BB Cycle Host s 0 104 Time and Date Settings Status Seepieg C Gersettiegs PST MACON HeeMeESS Tane to nest sample Hot OSN Om IR Teese to Prime ENN Cr onzo an C scp Seen Gew Changes O Stop after menticurrent sample Most Computer Offload Or CIC rdeDato RA POF PIR Raw Piot Settings Monitor Files Cal RI R2 4 3 12 Plot tabs Monitor the collected data in counts from the sensor in the Raw Plot tab and collected data in uM mg L or mgP L in the PO Plot tab Do a right click in the plot window to select how to look at the data pr Status PO4 Plot Raw Plot Settings Monitor
69. pumps operate for a user specified number of cycles The manufacturer recommends 120 LSS Last Shutdown State Description Get the status of the last shutdown 43 Reference Argument 1 none Response The message ok if the shutdown was via the XIT command or power failure if the last shutdown was from a power fault ONT Run Time Power Consumption Description Get set the total on time for the sensor This number is usually reset when a new battery is connected Argument 1 The new on time in seconds Argument 2 S safety flag Response The current new on time in h mm ss The hours field is from 1 to 10 characters PRI Prime Description Do the priming sequence immediately Argument 1 1 starts the priming sequence O starts the flush process while the priming sequence occurs Response The status of the priming sequence The message on or off will show if no arguments are made The message priming shows if priming is started RUN Run Description Start a new data sample sequence now If NOS is 0 it will automatically set to 1 Argument 1 P start a priming sequence Response The message Running shows if a data sample sequence is started The message Priming shows if a priming sequence is started SDS SDI Status Description Get the status of the SDI interface board Argument 1 none Response VER n n c enab
70. r may not go to full power if this is set to a value of less than 3 seconds 7 Use the Prime checkbox to show the effects of a prime cycle on the deployment calculator 8 Push Apply New Settings when the selected values have been entered into the Proposed variable boxes 4 3 13 2 Cycle deployment calculator Enter a short description of your topic here optional This is the start of your topic 4 3 13 3 Pump controls Pump Controls S Cal Ri R2 Pump Num Pumps Run Pump s Stop Use the pump control boxes to select a pump to operate Enter the number of times to operate each of the pumps that are selected in the variable boxes and push Run Pump s to start the pump sequence To see how many pump operations are left in the current sequence go to the bottom of the Monitor tab and type PMP 4 3 13 4 SDI controls Sensors that have an SDI 12 interface have an additional 8 contact bulkhead connector The default address is 0 The user can change this address in the host software or at the set address command through the SDI port SDI 12 Address SDI Status Set SDI Address Get SDI Status 64 Reference The sensor does not need to be connected to an SDI 12 bus to change the address 4 3 14 Monitor tab This tab is generally used for troubleshooting Use the Monitor tab to look at the serial data that goes between the host PC and the sensor Copy and save the serial data in the w
71. r that is set to collect and send the collected data to a shore side database at regular intervals Other sensors on the mooring turn on for 2 minutes of every 15 The Cycle collects data once per hour Synchronous slave mode The sample rate of the sensor is synchronized with the controller The sensor collects data when signalled by the controller The controller supplies continuous power to the sensor Deployment the sensor is installed on a mooring with a system controller that is set to collect and send the collected data to a shore side database in real time SDI 12 mode The sensor operates in the synchronous slave mode through the SDI 12 port Commanded mode The sensor is connected to the controller and is under the control of the controller This mode has the most control over the sensor and also needs the most work to use 2 3 2 Set up for deployment Wear Personal Protective Equipment PPE to remove or replace cartridges PPE includes a lab coat or smock gloves safety glasses The sensor can be hung under a dock with a length of rope or installed as part of a larger system Operate the sensor 15 off vertical The manufacturer recommends full submersion of the sensor The sensor can operate in less water as long as the intake filters on the bottom of the sensor are submerged If they are not the sensor cannot flush air bubbles which can result in poor data quality Prevent the reagent cartridges from freezing and
72. rder sends ODO sensor sends 0 11 0708 06 1011 0503 12 234 0 9 11 8FZH lt CR gt lt LF gt Continuous Measurement aRO Continuous Measurement and Request CRC aRCO Response a lt values gt lt CR gt lt LF gt or a lt values gt lt CRC gt lt CR gt lt LF gt Notes sensor sends the last measurement data stored in the FIFO The format is the same as aDO after an aC or aCC command If there is no data in the FIFO the sensor sends the last data record sent the Continuous Measurement command does not start a measurement It sends data from a previous measurement aRO Example 1 address 0 values from the previous measurement sample date 11 0710 sample time 06 1213 run number 0505 PO concentration 12 890 units of PO concentration 0 last sample state 9 battery voltage 11 6 SDI recorder sends ORO sensor sends 0 11 0710 06 1213 0505 12 890 0 9 11 6 lt CR gt lt LF gt aRCO Example 2 address 0 values from the previous measurement sample date 11 0712 sample time 06 1415 run number 0507 PO concentration 12 456 units of PO concentration 0 last sample state 9 battery voltage 11 4 SDI recorder sends OM sensor sends 0 11 0712 06 1415 0507 12 456 0 9 11 4J W lt CR gt lt LF gt 54 Reference Stop Measurement aX_STOP Response aSTOPPED lt CR gt lt
73. reagents and calibration standard into the fluid passages Table 1 Equipment needed to prime the Cycle User supplied Manufacturer supplied 2 receptacles for water Regulated power supply 50 mL syringe Test cable PC Ya outside diameter OD tubing lt 1 m in length 1 Find the Y OD Y shaped tubing connected to the S mark on the sensor 2 Pull the tubing from the hose barb next to the S straight off the barb The barb is angled up Operation Figure 9 Y shaped tubing disconnected from S Unwind the exhaust tubing from the top of the sensor Put one end of the exhaust tubing in an empty receptacle Fill another receptacle with 150 mL or more of DI water Connect the 50 mL syringe to the length of 1 4 tubing 7 Put the end of the tube in the de ionized water and use the syringe to fill it with water sarao Figure 10 Tube filled with DI water 8 Make a kink in the tube near the syringe so that no water drains from the tube and remove the syringe Figure 11 Syringe removal from filled tube 9 Keep the tube with a kink in it and push it onto the hose barb near the S mark Make sure there is no air in the tubing or in the hose barb 10 Connect the manufacturer supplied test cable to the sensor a power supply that can provide 2 amps and the host PC The user will need a serial to USB adapter cable 10 Operation Figure 12 Sensor ready to be primed
74. ricate bulkhead ConNector coociconionoicccoimariincn tc ce 31 A a a ea aa se thc danandotead denne a A E 31 3 3 Clean sensor flow paths with cleaning solution oooccconnoconcccnnnnnnnccononccnnnnnnnnnnnanonnncnnnnnnnnnnnnnns 31 3 3 1 Clean flow paths with bleach solution oooooononicnnnnnnnnnnncnnncnnicnnirrnir 33 3 4 Flush cleaning solution from flow pAthS ooooninonoccccnnnnnnncoconcccnnnnnonnnnenonnnnnnnnnnnnnnnn na nnncnnnnnnnnnns 34 3 5 Replace reagent cartridges irrist ult iii asc 34 3 6 Replace intake filter and SCreen ooooococccccccciconicononenonenenenoninnnenonnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 35 3 7 Prime sensor with VaCcuUuUM oooooooococccnnccnncnoncnoncnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnrnnnnnnnnnninos 35 3 8 Send reagent cartridges back to manufacturer oooocccccccononcconcccccccnnnnnonnccnnncnnnnnnnnncnnnnnnnnnnannnnnnos 36 3 9 Sensor Storage iarere Goer de ce event a dea Ae Sedna estes a a hie A inde E SE aa 37 CAS IESI o A T O 37 Table of Contents 332 Long term SlOrage iii ais 37 Section Referente aa 39 4 1 Description of nutrient units ooooconncnnnnnnnnnnncninnncnnncnn rr 39 4 2 GY SIS c mmaNdS eo sche aca ai eae Said arte aa na reece Dee dae nec dee da Bee A A TA 39 4 2 1 Configuration commandS 24 tein sadist tc crack abans 40 42 2 OPSration COMMONS miii a adds 43 4 2 3 Fle command Sirene an en n rea a diaz 45 4 2 4 Miscellaneous COMMANdOS o
75. ription Erase the specified file or files from the flash drive Wildcards and are permitted Argument 1 The file or files to be erased Response n file s erased n the number of files erased The response ends with two lt CR gt lt LF gt DIR Dir Description Get a list of the files in the directory Wildcards and are permitted The character any group of one or more alphanumeric characters The any single alphanumeric character Argument 1 The directory to list or D for the current directory Argument 2 The optional filename pattern to list For example txt Response A list of the directory with the specified filters If a file is being collected it will list on the last line separated by a space from the preceding Active file GET Get File Description Get the specified file or files Wildcards and are permitted The file move is stopped with an RS232 break signal of 500 ms or longer This is followed by the checksum for the characters that were sent before the sensor got the break signal Argument 1 The files to be moved Response All of the specified files will be moved A header line comes before each file The header line has the filename the number of the file being moved from 1 to n the total number of files moved the file size in bytes and the number of bytes that need to be moved This information is separated by a space The whole file is moved and is followed by the CRC 32
76. sed Current Proposed Interval 1 00 00 1 00 00 Max Runs 2615 Max Runs 2615 Cal Frequency 6 6 Days 109 0 Days 109 0 Num Samples Cal Used mL Cal Used mL 105 Low Power Idle 0 02 00 5 R1 Used mL R1 Used mL 244 Prime R2 Used mL A R2 Used mL 244 Total Effluent L Total Effluent L 177 Pump Controls Charge Used Ah Charge Used Ah 99 S Cal Ri R2 2 An gt sn Pump FUF Limiting Factor Limiting Factor Rt Num Pumps Run Pump s Stop SDI 12 Address 0 SDI Status SDI ok Set SDI Address Get Spr status Current File f Set the Interval to 15 minutes 00 15 00 or as necessary for an overnight test Set the Cal Frequency to 1 Set the Num Samples to approximately 20 for an overnight test Touch or select any area within the window so that the software accepts the values from the steps above Check the Deployment Calculator in the Status tab to make sure there is enough reagent in the cartridges for an overnight test Push Apply New Settings The yellow highlights go away Go to the Files tab Make a folder for stabilization or laboratory sample collection for example Stable or Lab1 Note that the file name is limited to 8 characters Push Run A new Choose Run Option window shows Push Set Start Data and Time to select when the sensor starts to prime and run It takes approximately 12 5 minutes to prime the sensor
77. sensor before sending it back to the manufacturer Note The manufacturer will not accept sensors that have been treated with anti fouling compounds for service or repair This includes tri butyl tin marine anti fouling paint ablative coatings etc 3 Use the sensor s original ruggedized shipping case to send the sensor back to the manufacturer 4 Write the RMA number on the outside of the shipping case and on the packing list 5 Use 3rd day air to ship the sensor back to the manufacturer Do not use ground shipping 6 The manufacturer will supply all replacement parts and labor and pay to send the sensor back to the user via 3rd day air shipping 5 3 Waste electrical and electronic equipment Electrical equipment that is marked with this symbol may not be disposed of in European public disposal systems In conformity with EU Directive 2002 96 EC European electrical equipment users must return old or end of life equipment to the manufacturer for disposal at no charge to the user To recycle please contact the manufacturer for instructions on how to return end of life equipment manufacturer supplied electrical accessories and auxiliary items for proper disposal 71 General information 72 Sea Bird Coastal 13431 NE 20th Street Bellevue WA 98005 U S A sales 425 401 7653 support 425 401 7654 support sea birdcoastal com www sea birdcoastal com WET Labs Inc 2012 All rights reserved Printed in USA G
78. sh O LSS last shutdown state C NOS number of samples O SONT show on time C OPD output period O PMP operate pumps M PRI priming state C RAT get set baud rate F REN oldname newname F RMD remove empty dir O RUN run sample C SDA SDI bus address C SDO output format O SDS SDI status O SLP low power sleep C SPR prompt on off C STO store config info O STP stop sample C SUD start time date O TPC get total pump counts O TTS time til start F TYPE dump file s C UPC get ups count M VIN get input voltage O VOL get volumes pumped M VSN show version info O WKM get set wake mode O XIT exit to PicoDOS PMP Operate Pump s Description Operate the specified pumps for a specified number of cycles Files are not made and data is not collected Use the STP I command to stop a PMP command Argument 1 None to get the number of pump cycles left or a decimal code that specifies which pumps to operate Set a bit position to 1 to start the pump Response The number of pump cycles left Pumping Done pump operation is complete Code Amb Cal R1 R2 Code Amb Cal R1 R2 0 no no no no 8 no no no yes 1 yes no no no 9 yes no no yes 2 no yes no no 10 no yes no yes 3 yes yes no no 11 yes yes no yes 4 no no yes no 12 no no yes yes Reference 5 yes no yes no 13 yes no yes yes 6 no yes yes no 14 no yes y
79. so shipped to the user The user can check the calibration of the sensor and validate any lab prepared standards Contact the manufacturer to get more check standard 2 4 4 Solutions for in laboratory performance analysis Change to a new solution to analyze with the sensor 1 Disconnect the sample inlet tube from the S barb 2 Let the solution drain into the sample reservoir 3 Flush the inside and the outside of the tube with clean water The manufacturer recommends 18 MOhm 4 Shake to dry 5 Refer to the steps in Prepare to prime the sensor on page 9 and be careful to not make more bubbles in the intake tube Degassing sample can minimize the formation of bubbles 2 5 Data analysis 2 5 1 Files tab Use the software to get the data that is saved in the sensor 1 Start the software if necessary 2 Select the Files tab 22 Cycle Host File Tools Help COM Settings COM 5 Y Rate Time and Date Settings Get Settings PST MM DD YY HH MM 55 Host 01 04 12 15 04 00 Apply New Settings Cyde Clear Changes Host Computer Offload Dir C CycleData Status PO4 Plot Raw Plot Settings Monitor Files Cycle Files Instrument Files H E RUNI E RUNZ 00000004 TXT e 00000003 TXT e 00000002 TXT SUMMARY TXT e 00000001 TXT H E DATA Filter TxT 3 Push Refresh Root Directory Listing The files saved in the sensor will show in the Files tab Time to next sample Unknown
80. t window Save Analysis Set Push to save the data files that are loaded in the Raw Data Plot window By default the file is saved in a txt format in the same directory as the data As an alternative the user can save the file to another location on the PC Analysis set statistics for nnn samples The software calculates the average value for each Test value of the data that the user saved for analysis In the table below for example the software made an analysis of 26 data files Analysis set statistics for 26 samples gt tes Good Suspect sad missing All 46 2 42 3 11 5 0 0 Bubble 80 8 19 2 0 0 0 0 cov 100 0 0 0 0 0 0 0 Low Sig 100 0 0 0 0 0 0 0 Out Of 100 0 0 0 0 0 0 0 Mixing S 65 4 26 9 7 7 0 0 Cal Spike 20 0 40 0 40 0 0 0 4 3 16 1 Cycle QC example Go to the Tools menu then select Options or type Alt O to open the Options window The values in this table are the upper and lower limits for good suspect and bad data Low Si 170 750 co co Out Of 0 05 0 075 10 5 40 Mixing 00 00 30 100 Cal Spike 09 00 30 100 Reset to Defaults Definitions of Tests Bubble Spike the software calculates the transmittance data while the pump operates and looks for spikes that are an indication of bubbles The software then shows a cumulative value of spikes in the sample Good less than 300 counts e Suspect
81. ter the software will try to connect to the last port used Note There is a known issue with the serial port drivers on some PCs using the Windows 7 operating system The user must close the Cycle software to make an opened COM port available again 4 3 7 Operation values Change the parameters to collect data in the sensor ools Help A Settings Time and Dati Mm 6 v Get Settings PDT MMC te 19200 Apply New Settings Test MS Cycle 05 Clear Changes 1 Push Get Settings to get the operation values stored in the sensor unless the Deployment Wizard will be used right away The host will get the operation values from the sensor 2 Push Apply New Settings to send new values to the sensor The area around the buttons is yellow until the new values are sent to the sensor 3 Push Clear Settings to make sure there are no active changes 4 3 8 Date and time setup Push Set Date and Time at the Deployment Wizard tab to set the date and the time in the sensor from the host PC The user can also use the Control Panel of the host PC to do this 4 3 9 Modes of operation Status Sleeping ss Time to next sample 0 39 50 1 10 Time to Prime 0 09 50 1 09 Run ia Stop jil Sleep Stop after next current sample mr The sensor operates one of five modes Table 16 Sensor operation modes Mode Description Unknown The host PC has not yet received any information from the sensor Sleeping The
82. the number of data points plotted compared to the total data points received This is the counter on the upper left corner of the Plot tab Note If the sensor cannot calculate a phosphate concentration the output will be NAN not a number All NANs are counted as the total number of data points received No NANs are counted as valid data 4 3 12 2 Raw data plot tab 62 Look at the data in this tab as a quality assurance tool The raw data in counts is proportional to transmittance Reference e MAA Sono ETT E tras Status PO4 Plot Raw Plot Settings Monitor les 00 00 00 00 02 00 00 0400 00 08 00 00 08 00 00 1000 00 1200 00 14 00 00 10 00 00 18 00 00 20 00 Time From Sequence Start HH MM SS Run 87 Run 88 Run 89 4 3 12 2 1 Raw plot controls Control the number of plots to show in the Raw Plot tab and erase all plots from this tab Note that the default maximum number of raw plots is 6 The manufacturer recommends a limit of 10 plots Tene to next sample es Ee EH top after nestcrent sample Host Computer Offload Dir C CycleData Runt EJ Start Recording gt Stats pos Piot Raw Pt Settings Monitor Fites 00 05 00 00 10 00 00 15 00 Time From Sequence Start HH MM SS Run 87 Run 88 Run 89 4 3 13 Settings tab 4 3 13 1 Sample collection schedule The user makes decisions about the val
83. trient units express the amount of something usually moles or mass relative to the volume it is in Many researchers and scientists use micromoles per liter uM a unit that is independent of mass and useful for stoichiometric calculations Most freshwater monitoring programs and many researchers use units of milligrams per liter This unit is almost always expressed as milligrams of relevant atoms per liter for example milligrams of phosphorus P per liter rather than milligrams of phosphate per liter Although phosphate PO 4 is the most prevalent form of phosphorus this unit is frequently used as a means of easily keeping track of total phosphorus loading Because milligrams per liter is a mass based unit and the mass of P and PO are different this difference is very important to prevent mistakes Milligrams per liter is also typically referred to as parts per million ppm the mass of P relative to the mass of water The Cycle PO sensor measures soluble reactive phosphate and displays units in micromolar uM or milligrams of phosphorus per liter mgP L The Cycle PO sensor also displays units in milligrams phosphate per liter mg L or mgPO L MgPO L is not commonly used in environmental analysis Standards such as the Hach 0 5 mg L standard are sometimes expressed in this unit 4 2 Cycle commands The user can use commands as an alternative to the host software to communicate with the Cycle sensor Refer to the information be
84. uence Amb Min The mean value of the 6 transmittance signal counts that triggered the knee slope threshold reaction Step 3 part of a sample sequence Flush2 The mean value of the last 10 transmittance signal counts in the ambient flush Step 6 part of a sample sequence Cal Min The mean value of the 6 transmittance signal counts that triggered the knee slope threshold reaction Step 8 part of a sample sequence Remaining The number of samples left as set by the NOS command Dia1 2 Reserved Diagnostic fields Table 10 Phosphate units Command Designator Description EUFO uM Micro molar phosphate concentration EUF1 mg L Milligrams per liter of reactive phosphate EUF2 mgP L Milligrams of atoms phosphorus per liter measured in the form of reactive phosphate Table 11 Step values Index Step Index Step 0 Initial flush 5 Mid flush 2 1 Ambient read 1 6 Ambient read 2 56 Reference Table 11 Step values continued 2 Mix ambient 7 Mix spiked 3 Ambient PO read 8 Spiked PO read 4 Mid flush 1 9 End flush A normal run completes in Step 4 A spiked run completes in Step 9 4 3 2 Raw transmittance output Table 12 Raw transmittance fields Field Description mm dd yy The sample date hh mm ss The sample time signal The transmittance signal counts 0 4095 run The run counter Increases in increments of one for each sample sequence step An index count
85. ues for the sensor to use in the Sample Settings area of the Settings tab The user can also use the Deployment Wizard from the Tools menu to automatically set these values 63 Reference Status PO4 Plot Raw Plot Settings Monitor Fi Set Date and Time Units uM v Sample Settings Current Proposed Interval 0 40 00 0 40 00 Cal Frequency 1 11 Num Samples 10 110 Low Power Idle 0 00 30 0 00 30 Prime O 1 Push Set Date and Time to adjust the sensor to the same time as the host PC Enter the type of units in which to save data 3 Enter the interval for the sensor to sample The format for this is hours minutes seconds The sensor takes approximately 30 minutes to complete a sample sequence If the interval is very small the sensor will apparently collect data continuously To take a sample once every other day for example set the interval to 48 00 00 4 Enter how frequently the sensor will do a calibration spike after an ambient sample Change this value to 6 so that the rest of the sequences to not do the cal spike 5 Select the number of sample sequences or the start date and time The number of samples can be set from 1 to 32767 A value of 1 or a blank will set the sensor to do sample sequences as long as it has power 6 Select how long the sensor will stay at full power draw The default is 2 minutes The sensor then goes to a low power mode until the next sample sequence starts Note The senso
86. use a sun shade to keep the cartridge temperatures below 35 C Make sure that the waste tubing does not have kinks in it when the sensor is deployed Operate the sensor at least 10 cm above the bottom of a body of water This allows for circulation around the intake filters Do not use the handle to deploy the sensor Make sure that the electrical cables have no tension The user may attach the sensor to a structure such as a mounting bracket Make sure to have a backup attachment for safety 15 Operation e Use braided line rope not twisted nylon to support the cables bracket aluminum alignment pin base plate Mounting considerations A e brocket assembled as shown on the left can be bolted to a WET Labs IOP Profiler cage using 1 4 20 hardware Offset holes on the bracket allow mounting in several locations within the cage 2 The aluminum bracket is easy to drill and modify on site for custom installations Attaching es to the Bracket E P ign the Owel pin n the bracket with the drain slot on the bottom of the instrument This aligns the intakes to be clear of the bracket 2 Set the instrument down over the pin and rock it back against the saddles The top of the shell will slide underneath the restraining bar 3 Wrap the polypropylene straps around the instrument adjust them to length and snap them together They should be very tight It may be easier to adjust the length before snapping the buckle

SBC CyclePO4=3=Hach A4 Portrait User Manual - Sea

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