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Small Animal User Manual

1. Wireless Solutions for Physiological Monitoring User Manual for Small Animal Systems 1 Telemetry Research User Manual for Small Animal Systems v1 3 Important Information Telemetry Research Limited TR believes that information in this user manual is correct at the time of publishing In the event errors may exist TR reserves the right to make changes to future editions without notice to holders of this edition The reader should consult TR if errors are suspected In no event shall TR be held liable for damages arising out of the use of this document or information contained within Except as specified below TR makes no warranties express or implied and specifically disclaims any warranty of the products fitness for a particular purpose The customer s right to recover damages by fault or negligence of TR are limited to the amount paid by the customer Any action against TR must be brought within one year after the cause of action accrues TR will not be held liable for any loss of data research funding or profits and neither will it be liable for incidental or consequential damages even if advised of the possibility thereof TR expresses that its products are for research purposes only They are not designed with components and testing intended for use in medical treatment or diagnosis of humans or animals and doing so may result in serious harm Warranty All products manufactured by Telemetry Research Limited TR
2. Serial number of SmartPad TR180 Start Search for Diagnostics Smart Pad Current transmission channel of SmartPad 2 Firmware Version i 3 Charging Field 4 Atmospheric Pressure i Go Sensor function V Change transmission channel to Charaing field 5 Temperature normal 6 Power consumption normal 24 Telemetry Research User Manual for Small Animal Systems v1 3 Calibration values Prior to shipping all Telemeters with a pressure sensor are calibrated under the following conditions Hydrated for 48 hours Temperature 37 C In the dark no ambient light Clean i e no blood products on sensing tip O O O 0 Under these conditions the voltage analog output from the SmartPad corresponds to the following pressures 1V 0 mmHg 2V 100 mmHg If confirming checking the pressure offset the above conditions need to be replicated as the offset will be affected by hydration temperature light and blood products on the pressure sensing tip Telemeters are shipped sterile and dry when implanted pressure values recorded in the first 48 hours after surgery may have an offset of up to 20 mmHg Prior to shipping all Telemeters are calibrated so that the voltage analog output from the SmartPad corresponds to the following voltages OV 2 mV 2V 0 mV 4V 2 mV Prior to shipping all Telemeters are calibrated so that the voltage analog output fr
3. Goa soss si 772298 1462 29 o o snzeoto1e05 so tamsapasia 412i 211 so 4905 143 Good sosi sos zszasi 1353 soo o o snzeoto1695 so TRMs4P 2519 412i 211 oo s4ea 157 Good sosz7 613 sessae 2170 286 o o a1 90 5205 149 Goa sorz 5001 764006 1401 sor o o snzeoto16s6 60 rrwsap cs19 s21 21 oo sen 132 coos __aa75__ soss zesas2 1397 sors o o snzeoto16s6 zo tamsapasia 4121 211 oo 5195 147 Good sosa _ ston areas 1593 2002 o o snzeoto1es6 so TRMsaP 2s19 412i 211 oo 5205 149 Good soss soos 7esoss 14283 2006 o o snzroto1696 o0 tamsap2sia 412i 211 oo aso 140 Goa sosz7 613 esses 1411 2086 o o snzieot0 1636 100 TRMs4P 2519 4102 210 oo s0es 145 Goa sose si 7e22ss 1388 288 o 1 37 Telemetry Research User Manual for Small Animal Systems v1 3 SmartPad Diagnostics Using the Configurator System the user can investigate the status of their SmartPad To run diagnostics enter the serial and channel numbers of your SmartPad and press the Start diagnostics button on the SmartPad Configuration amp Diagnostics page Please note that you cannot record data from your Telemeter using a SmartPad that is in Diagnostics mode ConfigSoft nn File F Help H Getting Started Telemeter Configuration amp Diagno
4. Below is an example of data from the log file created by the Telemeter Diagnostics function Several of the parameters are reported in both standard units and as Raw values The Raw columns are the digital bit values transmitted which are then converted to real useable information This information is likely to only be of use to support staff at Telemetry Research There will also be some more information at the end of the list of parameters This is intended to only be of use to Telemetry Research Number of Charging status Absolute pressure attempts to Time of l l Battery status is Charging field If has 3 possible Temperature detected by sensor Only valid for dual transmit a day based Time since Telemeter presented as a field v gt battery v values Good of the sensor i e not adjusted for Pressure Telemeters packet of on PC diagnostics serial voltage Raw and then charging will Moderate or inside the atmospheric values can be ignored data clock began number as a percentage occur Not charging Telemeter pressure for all other models TR use only m m p Ey N N C6 N N N N Elapsed Time Battery Battery Field Temperature Pressure1 Pressure2 Time seconds Telemeter ID V B Raw V F Raw Charging Status C T Raw mmHg BP1 Raw mmHg BP2 Raw Retransmit 3 12 2010 16 36 TRM54P 2519 4121 211 90 4 866 30 88 5104 864 056 2197 2084 o o snzeoto1e95 20 tamsap 2sia 412i 211 oo sasa 157
5. 1 Before using a SmartPad with a Telemeter you need to configure and pair each SmartPad with the same Channel of the Telemeter to be used Front Back Power socket Analog Outputs Status Light information Orange The SmartPad is turned on but is not communicating with a Telemeter Green The SmartPad is communicating with a Telemeter on the same Channel Flashing orange The SmartPad is in diagnostic mode No Telemeter data will be output Red The SmartPad has automatically disabled the charging field due to detection of a high current In this mode data output will continue but battery charging is not occurring This may be due to being placed too close to a metal surface The SmartPad can be reset by unplugging the power and plugging it back in 1 Connect the supplied Power Supply to the Power Socket on the back of the SmartPad 2 The Status light will be Orange 3 Use the Configurator System to select the Channel to match the Telemeter being used 4 Status light will change to Green when SmartPad and Telemeter Channel are matched 5 By default the charge field of the SmartPad is enabled if you need to disable the charge field use the Configurator System 12 Telemetry Research User Manual for Small Animal Systems v1 3 Analog Outputs After pairing the SmartPad Telemeter Channels the SmartPad automatically detects the type of Telemeter it is communicating with and the analog outputs on the back will correspond to the follow
6. 2 Enter the serial number of the SmartPad found on the front and back of the SmartPad 3 Enter current Channel number of the SmartPad first use Channel 1 4 Enter or select a new Channel number for the SmartPad should be same as Telemeter being used 5 Ensure Change transmission channel to is checked 6 Click Go to activate the new Channel 7 Click OK to confirm your choice Coniston gi ee New Configuration SmartPad 5617 will be changed from channel 1 to channel 3 Click OK to confirm 21 Telemetry Research User Manual for Small Animal Systems v1 3 8 A message confirming that the SmartPad channel has been changed will appear Record the SmartPad serial number and new Channel number for future reference ConfigSoft p SmartPad is now channel 3 This SmartPad can operate with a Telemeter on channel 3 9 If using a Telemeter on Channel 3 in this example the SmartPad Status light should be green the Telemeter and SmartPad are on the same Channel paired and able to communicate for data collection and transmission If the Configurator cannot communicate with the SmartPad you will get the following error message ConfigSoft Communication failed 1 Make sure SmartPad is powered on and close to Configurator 2 Make sure serial number and channel are correct Then retry Click OK and perform the following then retry e Check that the light on the front of
7. Telemeter move any other Telemeters out of the detection area more than 5m away 23 Telemetry Research User Manual for Small Animal Systems v1 3 The Search for Telemeter function does not find the Telemeter What do do now There are 3 possible explanations 1 The Telemeter is in Safe Mode 2 The battery of the Telemeter is too flat and the Telemeter has switched to Safe Mode 3 The Telemeter is too far away from the Configurator Solutions 1 Move the Telemeter closer to the Configurator and run the search again 2 Place the Telemeter on a SmartPad with an active charging field Run the search again Now that the Telemeter has been found check the battery level see Telemeter Diagnostics section If the battery level is below 30 the Telemeter needs to be charged before the Channel can be changed Place the Telemeter on a SmartPad with the same Channel and leave it to charge until the battery level shows gt 30 What if do not know the Channel setting of a SmartPad Go to the SmartPad Configuration amp Diagnostics page Enter the serial number of the SmartPad and press the Search for SmartPad button The serial number of the SmartPad can be found on the front and back of the SmartPad a EEE R ConfigSoft iy a D E i File F Help H SmartPad Configuration amp Diagnostics Getting Started Telemeter Configuration amp Diagnostics Help amp Support 1 Serial Number
8. User Manual for Small Animal Systems v1 3 Telemeter Removal and Preparation for Reuse If the user takes care the Telemeters are designed for reuse many times After implantation for a long period of time it is common to find the catheter electrodes and Telemeter body covered in connective tissue When removing the Telemeter take care to avoid damaging catheter electrodes or Telemeter body Never use a scalpel to cut tissue from around or above the Telemeter as it could damage the body Extreme care should also be taken not to damage the pressure catheter and sensor tip The Telemeter should be soaked in cleaning detergent see below After soaking the sensing tip should be gently wiped with a soft cotton gauze pad or swab sticks to remove any remaining film or deposits Care must be taken not to damage the sensing tip do not pick up the catheter by the sensor tip or have any sharp objects near this tip Taking care during removal and cleaning of the Telemeter and pressure catheter will mean that many recordings from a single Telemeter can be made before needing a replacement Cleaning 1 After removing the Telemeter from the animal immediately soak it in a beaker or dish filled with fresh saline or distilled water Keep soaking until you are ready to continue cleaning DO NOT allow it to dry 2 Soak in a recommended enzymatic cleaning solution listed below This is essential to prevent protein build up on the pressure sensor Without
9. acquisition telemetryresesrch com Telemetry Research User Manual for Small Animal Systems v1 3 Small animal systems Signal Telemeter Receiver Configurator ea a charger Pressure only e g BP LVP bladder DVEINS e g BP amp LVP TRM54PP Pressure biopotential TRM54PB e g BP amp ECG onfigurator Small animals SmartPad TR180 TR190 gt 200 gm ie rats oe Dual Biopotential haa iets TR50BB and guinea pigs pigs e g ECG and EEG Single biopotential e g EMG ECG TR162 receiver See SNA BP User manual for further SNA amp BP TR46SP TR802 charger information Telemetry Research User Manual for Small Animal Systems v1 3 Configurator System Setup Introduction The Configurator System Configurator hardware and ConfigSoft wirelessly communicates with all Telemeters and SmartPads The system allows the user to 1 Change configure Channel frequencies Turn Telemeters into Safe Mode for storage Check Telemeter battery level Enable or disable the charging field of the SmartPad Run Telemeter and SmartPad Diagnostics Red Rum A single Configurator System is only required for each laboratory and is NOT required for data acquisition NOTE ConfigSoft must be installed on the PC before connecting the Configurator hardware System requirements Windows XP Vista 32 bit or 7 32 amp 64 bit amp internet connection is necessary to access and install any drivers not
10. already installed as well as to access TR Help 1 Insert the TR USB drive containing ConfigSoft into a PC USB port 2 Open the folder ConfigSoft 3 Double click on setup to start the installation Depending on the files and drivers already installed on your computer some of the following steps may not be necessary 4 Accept the license agreement for NET Framework 13 ConfigSoft Setup lowing componerts Microsoft NET Framework 4 Client Profile x86 and x64 Please read the folowing license agreement Press the page down key to see the rest of the agreement MICROSOFT SOFTWARE SUPPLEMENTAL LICENSE TERMS MICROSOF T NET FRAMEWORK 4 FOR MICROSOFT WINDOWS OPERATING SYSTEM a View EULA for printing Do you accept the terms of the pending License Agreement you choose Dont Accept install will cose To instal you must accept this agreement 6 Telemetry Research User Manual for Small Animal Systems v1 3 5 The Microsoft NET Framework will be downloaded from the internet if not already installed this may take some time Follow all instructions to complete the installation 6 ie Co nfigSoft Welcome to the ConfigSoft Setup Wizard The installer will guide you through the steps required to install ConfigSoft on your computer WARNING This computer program is protected by copyright law and international treaties Unauthorized duplication or distribution of this progr
11. in power B Light on front will display solid orange 4 Activation of Telemeters Do not open the Telemeters from their sterile packaging B Place only one Telemeter package on the SmartPad ensure any other Telemeters are 2 meters away C The SmartPad will turn the Telemeter into active mode and will start transmitting data The green colour of the LED on the front of the SmartPad indicates that ii it has identified a Telemeter on the same transmission channel Orange indicates no Telemeter could be found on that channel DO NOT activate more than one Telemeter until the first Telemeter has been changed to another channel Ea a4 A i E SS o CA FTT OENE 9 Storage of Telemeters Remember Telemeters are fragile and cannot be refurbished if damaged Always store them in the containers provided Use the Configurator software to place Telemeters into safe mode for storage or when animals will be away from the SmartPad for an extended period 5 Pair Telemeters and SmartPads using Configurator software and Configurator All Telemeters and SmartPads are initially configured to transmit and receive data on channel 1 Reset and pair multiple Telemeters and SmartPads to one of 16 frequencies 10 Questions support support telemetryresearch com Once the channel has been set switch the Telemeter to safe mode using ConfigSoft to conserve battery life The Configurator is not required for data
12. need to be paired Please ensure that only one SmartPad and one Telemeter are configured and paired at any one time All SmartPads and Telemeters are shipped on Channel 1 as default When pairing a Telemeter SmartPad make sure any other SmartPads on the same Channel are turned off until needed and any other Telemeters on the same Channel remain at least 2 m away from any active SmartPad 1 Turn on the SmartPad Status light should be orange Insert power plug here 2 Place a Telemeter still in its sterile packaging on the SmartPad This will automatically turn the Telemeter into Active Mode data transmission active 3 If the Status light on the front of the SmartPad glows green then the Telemeter and SmartPad are on the same Channel and paired for data collection If the SmartPad Status remains orange the SmartPad and or Telemeter need to have their Channels changed paired using the Configurator System Changing Telemeter Channel 1 After activating remove the Telemeter from the SmartPad and place it 1m away from the SmartPad 2 Open ConfigSoft 3 Click on Telemeter Configuration and Diagnostics 18 Telemetry Research User Manual for Small Animal Systems v1 3 Telemeter Configuration and Diagnostics RCo igSoft File F Help H Getting Started Telemeter Configuration amp Diagnostics SmartPad Configuration amp Diagnostics Help amp Support 1 Serial Number 2 Fi
13. needs to be at least 10 cm from the shelf surface SmartPads should be on shelving so that there is a vertical separation of at least 40 cm between SmartPads There should also be at least 10 cm separation between the top of a metal cage lid and the SmartPad on the shelf above The photo below shows an example of how to setup your SmartPads to record and charge from 6 rats in standard cages age 14 Telemetry Research User Manual for Small Animal Systems v1 3 The Telemeter Telemeters are shipped sterile ready for implantation ALL Telemeters are shipped on Channel 1 and multiple Telemeters need to be configured to different Channels before use There is no need to unpack the Telemeter from the sterile packaging to change the Channel The Telemeters are fragile electronic devices Care must be taken not to damage the Telemeter body catheter or electrodes No repair is possible Please read the section about Handling and Care of your Telemeters Depending on the model your Telemeters may look Slightly different to those pictured throughout the manual however their care handling preparation and use are the same Telemeters are available in different combinations with pressure sensors and or electrode leads Model number Charging coil Antenna Serial number Pressure Sensor Telemeter models that include pressure measurement are fitted with a solid state pressure sensor from Millar Instruments The sensor is at the end
14. other than 3 as in this example the Status light will be be orange To change the SmartPad Channel read on Troubleshooting Configurator Telemeter If the Configurator cannot communicate with the Telemeter an error message will appear an i Communication failed 1 Make sure Telemeter is powered on and close to Configurator 2 Make sure serial number and channel are correct Then retry Solution Do the following and try again e Check that you have entered the correct Telemeter Serial number Check that you have entered the correct Telemeter Channel number Move the Telemeter closer to the Configurator Check the Telemeter is not on an active SmartPad Move other Telemeters away 20 Telemetry Research User Manual for Small Animal Systems v1 3 SmartPad Configuration and Diagnostics 1 To change the SmartPad to work on the same Channel as a known Telemeter click on SmartPad Configuration and Diagnostics Tab 1 ConfigSoft File F Help H Getting Started Telemeter Configuration amp Diagnostics SmartPad Configuration amp Diagnostics n Help amp Support Serial number of SmartPad TR180 Ce 1 Serial Number S p Current transmission channel of SmartPad C 3 2 Fir ET EER 3 Charging Field O transmission channel to CD 4 Atmospheric Pressure Sensor function E Charging field 5 Temperature normal 6 Power consumption normal
15. used for the sterilization of heat sensitive medical equipment such as Telemetry Research Telemeters When used properly chemical sterilants will destroy all viable forms of microbial life Glutaraldehyde may be used to sterilize the Telemeters Glutaraldehyde must be diluted to 2 before use Fisher Scientific is a vendor that supplies Glutaraldehyde catalog NC9795446 Check your local chemical supply company for availability Do not use gluteraldehyde solutions containing surfactants e g Cidex 7 or Cidex Plus 28 Day Rinse with Sterile Saline The Telemeter should always be washed in sterile saline before implantation to remove all traces of the sterilant Use it to temporarily store lt 48 hours the Telemeter aseptically until surgical implantation Ethylene Oxide Gas Sterilization Sterilant used is Ethylene Oxide Gas These sterilizers operate at a low temperature of 55 C and use chemicals as the sterilant Items are packed and dry at the end of the sterilizing cycle Telemeters are suitable for Ethylene Oxide sterilization as long as they are not subjected to temperatures above 60 C Customers are liable for product repair if recommended products and instructions are not used or followed If there are any questions about a chemical or procedure please contact Telemetry Research support telemetryresearch com Warning Some examples of chemicals that will cause damage to Telemeters include but are not limited to alcohols phe
16. And Handing ccccccccecccceeeeeceeeeeceeeeceeeeseeesseeeessaesessaeeesseeeesaees 32 Telemeter Removal and Preparation for Reuse cccsececeeeeeeeneeeceeeseeeeneeeseeeeees 33 USN ZA ON ea E 34 Telemeter DiAQnOStiCs ccccccseccescccsceceeeseeeecececseeceueceueecseeseueseeesseeseeeseuseneeessensags 36 Mla ad DAINO Si CS aa cece cece cece cece ccquasencncsacqeecneececeqeescececstetazaveccsarerasateeatezctceaueeeeate 38 SmartPad Technical Specifications ccccccccseececeeeeeceeeeeceeeeeseeeeseeeeeeseeeesseeeeeaeeeeeas 40 Telemeter Technical Specifications erossa a EEE 41 Oxygen Telemeter Technical Specifications cccccceccccceeceeseeeeseeeeesseeeesaeeeeseeeeeeas 42 Frequently ASked OUCSIOMS sire taierctcsatctcioraietcterabopaiatetsscieteletalapatetaletaiencietelatetes ard 43 Contact Information cccceecccceeececeeeeeceeeeeseeeeeseeeeeseeesseeeessaeeessaeeessaeeesseeeseeeeeseeeeeas 44 To all users We pride ourselves on helping you achieve results If you have any questions related to the operation of your system please contact us support telemetryresearch com We are here to help 1 Telemetry Research User Manual for Small Animal Systems v1 3 Introduction All small animal Telemeters use a unique patented inductive power transfer system for recharging and making long term recordings in animals 200 grams and over Please note that if your system has been customized in any way par
17. C 1V output 20 C D 1 Telemetry Research User Manual for Small Animal Systems v1 3 Oxygen Telemeter Technical Specifications The technical specifications in the table below are for the TRM57Y Tissue Oxygen Telemeter Potentiostat type Voltage controlled current source 0 65V 650mV 0 to 600nA 12 bit A D Coiled stainless steel 28 cm Set potential Potentiostat current measurement range Potentiostat resolution Potentiostat electrode leads Potentiostat electrode lead diameter 1mm Temperature operating range 20 to 41 degrees Celsius 2000 Hz Cut off frequency 1000 Hz Up to 5 m range may vary depending on laboratory configuration Fully digital at 2 4 GHz 16 transmission frequencies are available user set 200 gm Silicone Sampling frequency Low Pass filtering in telemeter Transmission range Transmitted signal Channels Minimum animal weight Outer material Weight Approximately 12 5 gms TR 5 series Telemeters are designed to operate continuously when near a SmartPad If the animal is away from the SmartPad charging field or the Battery life charging field is turned off battery life is 4 hrs in Active Mode and approx 6 months in Safe Mode Minimum 5 hours Activation via the SmartPad and deactivation via Configurator System User required to compensate for the individual Carbon Paste Electrode sensitivity coefficient supplied with each Blue Box electrode Sensitivity Recharge t
18. MPLANT MONITOR DISCOVER 1 Install Configurator software prior to connecting to computer System requirements Windows Vista XP or 7 32 bit 64 bit Windows 7 amp internet browser Internet access is required to install all drivers and utilize help functions Insert USB drive and run ConfigSoft setup 6 Implant Telemeters Telemeters are normally shipped sterile and ready to implant Surgical guides are now available online For access please email info telemetryresearch com 2 Configurator Setup The Configurator enables users to set the ma transmission channel of Telemeters and SmartPads The Configurator wirelessly communicates to the Telemeter and SmartPad 7 Connect Smartpads to data acquisition system Connect SmartPad to a data acquisition system using the BNC outputs Each SmartPad has three analog outputs which are automatically set when the particular Telemeter has been paired Attach aerial to rear of blue Configurator case Connect Configurator to computer via USB cable 3 SmartPad Setup The SmartPad acts as a universal inductive recharging platform and receiving station for all small animal Telemeters TRS series 8 Acquire data When a cage containing the animal is placed on the SmartPad and inductive power is on the Telemeter will be activated and data transmission will occure Continuous 24 7 data collection and charging is from multiple animals is now available Ao A Plug
19. Raw PWM Field 8 12 2010 9 12 10 TR180 100 755 918 403159 37 25 745 345 08 70 183 Enabled 8 12 2010 9 12 20 TR180 100 755 918 403159 37 25 745 350 01 71 183 Enabled 8 12 2010 9 12 30 TR180 100 755 918 403159 37 35 747 345 08 70 183 Enabled 8 12 2010 9 12 40 1TR180 100 755 918 403159 37 35 747 354 94 72 183 Enabled 8 12 2010 9 13 50 1TR180 100 755 858 403127 37 35 747 350 01 71 186 Enabled 8 12 2010 9 13 60 TR180 100 755 955 403176 37 35 747 345 08 70 183 Enabled 8 12 2010 9 13 70 TR180 100 755 97 403185 37 35 747 350 01 71 183 Enabled 8 12 2010 9 13 80 TR180 100 755 933 403165 37 3 746 350 01 71 182 Enabled 8 12 2010 9 13 90 TR180 100 755 865 403129 37 25 745 345 08 70 183 Enabled 8 12 2010 9 13 100 TR180 100 755 895 403145 37 35 747 354 94 72 183 Enabled 39 Telemetry Research User Manual for Small Animal Systems v1 3 SmartPad Technical Specifications SmartPad Contains ambient pressure monitor outputs standard analog voltage compatible with any data acquisition system recharges the Telemeter battery Weight 2 5 kg Dimensions 400 w x 450 d x 60 h mm Cage platform dimensions 295 w x 425 d mm Power input 100 240 V 50 60 Hz Max Power draw Temperature Operating Range 10 to 40 degrees Celsius Output Connectors BNC x3 Low Pass filtering on signal outputs Cut off frequency 1000 Hz 40 Telemetry Research User Manual for Small Animal Systems v1 3 Telemeter Technical Specifications The technical specifications in the table bel
20. am or any portion of it may result in severe civil or criminal penalties and will be prosecuted to the maximum extent possible under the law 7 Click Next to use the default folder or choose another folder to install ConfigSoft Note We suggest that you choose the option to install ConfigSoft for anyone who uses this computer ie ConfigSoft Select Installation Folder The installer will install ConfigS oft to the following folder To install in this folder click Next To install to a different folder enter it below or click Browse Folder C Program Files T elemetryResearch ConfigS oft Install ConfigS oft for yourself or for anyone who uses this computer Choose this option Everyone Just me 7 Telemetry Research User Manual for Small Animal Systems v1 3 8 9 10 11 Confirm installation Click Next ie ConfigSoft Confirm Installation The installer is ready to install ConfigS oft on your computer Click Next to start the installation Install the Driver for the TR190 Configurator Click Install sa TR190 Configurator Driver Installer Telemetry Research Limited TR190 Configurator Installation Location Driver Version 6 1 C Program Files TelemetryResearchi TR 190Driveri Change Install Location Cancel Ignore the software Windows Logo warning ConfigSoft and drivers are safe to install Click Continue Anyway Software Installation A Th
21. are warranted against defects in material and workmanship for a limited period Telemeter sensors and electrodes have a manufacturer s warranty covered on first use or Out of box basis The Telemeter battery is covered by a one 1 year warranty All other products manufactured by TR are covered by a three 3 year warranty All TR warranty periods take effect from the date of delivery to the user as evidenced by receipts or other documentation Third party equipment is covered by the manufacturer s warranty Accessories and consumable products are not covered by this warranty The warranty expressed herein does not cover damage caused by failure to follow instructions for use owner s abuse misuse or negligence user tampering or modifications power failures or surges and events or accidents such as flooding or fire outside the reasonable control of TR In no case shall liability exceed the purchase price of the original product Any product that is found to be defective within the warranty period while operated in accordance with instructions for use and specifications will be replaced or repaired at the option of TR at no charge The customer is responsible in notifying TR immediately of a problem within the warranty period All returned products should be packed safely preferably in its original packaging and sent to TR at his or her expense TR will pay return to customer shipping costs Service Within the limits of the TR warran
22. automatically enter Safe Mode Charging y the Telemeter detects a charging field from a SmartPad and charging is occurring x the Telemeter cannot detect a charging field from a SmartPad and is not charging A Telemeter will continue to run off its battery for approximately 4 hours if fully charged Troubleshooting Charging there are a number of possible situations for a next to Charging 1 The Charging field of the SmartPad has been disabled using Configurator System 2 The Telemeter is not within 5 cm of the charging surface of a SmartPad 3 The SmartPad has temporarily turned the charging field off for temperature update 4 The Telemeter is in an area of the SmartPad with low field strength or it may not be parallel to the SmartPad surface therefore in the wrong orientation to receive charging from the field efficiently Pressure sensor function This only applies to Telemeters with a pressure sensors e g TRM54P TRM54PB etc y the pressure sensor is functioning normally x a problem has been detected with the pressure sensor Oxygen sensor function v the oxygen sensor is functioning normally x a problem has been detected with the oxygen sensor Please contact Telemetry Research for further assistance support telemetryresearch com 36 Telemetry Research User Manual for Small Animal Systems v1 3 Telemeter Diagnostics Log file The log file Saved as coma separated values can be opened in a text reader or Excel
23. ding on the model your Telemeter may look slightly different to images throughout this manual however all Telemeter models have the same basic construction and operation All Telemeters are shipped individually packaged and sterile ready for use All Telemeters will need to be activated and configured prior to implantation PLEASE NOTE the Telemeter does NOT need to be removed from its packaging for Activation and Configuration The SmartPad Do not operate the SmartPad on or within 10 cm of a metal surface or within 40 cm above or below another SmartPad or Wireless Power Charger The Configurator System The Configurator System includes the Configurator hardware and ConfigSoft Software Together they are used to wirelessly control Telemeters and SmartPads to e Change configure a Telemeter and SmartPad Channel frequency 1 16 e Run and provide diagnostic information about Telemeters such as battery charge and SmartPads e Place Telemeters into Safe Mode for storage e Control the charge field of the SmartPad ONLY a single Configurator System is required to control all Telemeters and SmartPads in a laboratory The Configurator System is not required for data acquisition IMPORTANT The ConfigSoft supplied on the USB drive must be installed on a PC before connecting the Configurator hardware to the computer 3 Telemetry Research User Manual for Small Animal Systems v1 3 Quick start guide for small animal systems I
24. e software you are installing has not passed Windows Logo testing to verify its compatibility with Windows XP Tell me why this testing is important Continuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the software vendor for software that has passed Windows Logo testing Continue Anyway STOP Installation Success Click OK x e j Installation completed successfully Telemetry Research User Manual for Small Animal Systems v1 3 12 ConfigSoft is now installed Click Close ig ConfigSoft Installation Complete ContigS oft has been successfully installed Click Close to exit Please use Windows Update to check for any critical updates to the NET Framework Close Connecting the Configurator Front Status light Antenna USB port connection The status light front on the Configurator indicates the following information Off Not connected USB cable unplugged Configurator is off Red Configurator is receiving power but not communicating with PC Green Configurator is communicating with the PC 1 Attach the antenna to the back of the Configurator 2 After installation of ConfigSoft connect the Configurator to your PC using the supplied USB cable The status light on the front of the Configurator should glow red indicat
25. eseeeteueessneeteneesaees 3 Configurator System Setup cccceecccseeccceeececseeeceeeeeseeeeeseeeeeseueeeseeeesseeesseeesseeesaaes 6 COnnigurator Software MStallatlo iaiaeiaeiaa i EEE OA 6 CORRES ING Me COMMON ANON eean EE E EE E E E 9 SmartPad Setup 0cccccccccecsecessseececscecsceseeccsseescsececseseesceeencsesertsesencseseccseeescsecseserese 12 THG TEINE oeira eaae E E E E E E E 15 Configurator Software Overview cccccseecccseeeeceeececeeeeeseeeeeseeesseeeeeseeeesseeeeaeeeeas 16 Pairing a SmartPad and Telemeter cccccccccceececceececeeeeeseeeeesseeeesseeeeeeaeeeseeeesaaes 18 Trouble shooting and frequently asked QUESTIONS cccccccccaeeeeeeeeeaeeeeeeeesaeeeeeeeesaeeeeeessaaeeeeessaaess 23 Calibration values ccccccccccecccceeececeeeeeceeceeseeeeesecesseeeessaeeessaeeessaeeessaeeeseeeeseeeeesaeeeeas 25 TEEME gener aS O an 26 Telemeter Modes cccccccccecccseeeceeeeceeeecseecsucecsueecsaeessueessueessueessueessasessusessaeessaeenaes 26 Placing a Telemeter into Safe MOde ccccccccceececceeeeceeeceseeeeesseseesseeeessaeeeseeeesaaes 28 Positioning the Telemeter During SUIGery cccccceecccceeeeeeeeeeeaeeeesaeeeeseseeseneeesaeees 29 COMSiPUCHOM Ol MeS i IP OUIGI6 Seecacccaceconcseaeconcstacanaeceseaececearconcatazeracacesanseccaccansetencoeeeee 30 surgical Mesh for BP Catheter Implantation ccccccccceeeeeseeeeeeeeeeeseeeeeseeeesaeeeeees 31 Telemeter Care
26. h over the aorta and catheter so that the slit sits around the catheter 5 Glue the mesh to the catheter and use small amounts of glue to attach the mesh to the surrounding tissue Take care not to put too much glue on the vessel or blood flow may be restricted when it dries BP catheter a Surgical mesh ONN T EERE m ATT TA E i tf tf ft tf ft tt ee Small dab of glue 31 Telemetry Research User Manual for Small Animal Systems v1 3 Telemeter Care and Handling Warnings The Telemeter pressure catheter if fitted is shipped with foam over the catheter tip to protect the sensor area The catheter tip should be kept in the foam when the Telemeter is being stored To protect the Telemeter it should always be stored in the plastic tray and box provided between uses Precautions Telemeters with Pressure Sensors Proper handling of the Telemeter and pressure catheter is very important to avoid damage and extend the Telemeter life Care must be taken not to damage the Telemeter body catheter or electrodes No repair is possible Use and Handling e Inspect the catheter using a microscope for damage cracking kinks etc before each use e Clean the Telemeter and sensor and electrodes immediately after each use See Cleaning Instructions e Do not touch the pressure sensor area with sharp objects Do not make sharp bends in the catheter e Do not apply direct pressure to the pressure senso
27. he Telemeter With the Telemeter in Active mode press the Search for Telemeter button on the Telemeter Configuration amp Diagnostics page of ConfigSoft The Configurator will search through each of the available 16 channels and report any Telemeters which are in Active Mode and within range and report the serial number and Channel File F Help H Getting Started Telemeter Configuration amp Diagnostics 1 Serial Number 2 Firmware Version Serial number of Telemeter 3 Battery Level Current transmission channel of Telemeter Battery Level acceptable 5 Charging 6 Pressure Sensor function Change transmission channel to if applicable 7 Potentiostat function if applicable We Search for Telemeter IS lt Avantan Select Telemeter to configure and press OK button Serial number Channel 5565 1 Searching for Telemeter channel 5 What if do not know the serial number of a Telemeter already implanted in an animal It is always best to record the Telemeter serial number and Channel at the time of surgery however if you do not know the Channel or the serial number of the Telemeter use the Search for Telemeter in ConfigSoft and use a process of elimination The Configurator will search through each of the 16 channels and report any Telemeters active and within range If you have more than one unknown
28. he animal For effective recharging the Telemeter body must lie parallel with the charging pad when the animal is mobile Charging will be less efficient if the Telemeter is placed on the side or back of the animal Telemeter Modes Each Telemeter has two modes Safe Mode deactivated not sending data Active Mode sending data Safe Mode All Telemeters are shipped in Safe Mode This mode is used when the Telemeter is being stored and has an extremely low current drain To place a Telemeter into Safe Mode o The Telemeter needs to be at least 1m away from any active SmartPad o Use the Configurator System to place the Telemeter into Safe Mode o Take care not to allow the Telemeter to come within 1m of any active SmartPad or the Telemeter will be activated by the field and will enter Active Mode This will happen regardless of which Channel the SmartPad Is configured to Long Term Storage Small animal Telemeters can be left in Safe Mode for up to 6 months Prior to placing the Telemeter into Safe Mode for storage the Telemeter should be fully charged If Telemeters are to be stored for longer than 6 months users should plan to put the Telemeters into Active mode fully recharge and place the Telemeters back into Safe mode 26 Telemetry Research User Manual for Small Animal Systems v1 3 Active Mode The Telemeter must be in Active Mode to transmit data To change the Telemeter to Active Mode from Safe Mode o The Telemeter can be activa
29. ime On off mechanism Calibration range is 1 2nA uMol Oxygen Signal 1V output OnA 2V output 200nA Temperature OV output 0 C 1V output 20 C Analog output calibration values iN 2 Telemetry Research User Manual for Small Animal Systems v1 3 Frequently Asked Questions Battery and recharging Would the animal be harmed by staying in the SmartPad charging field There are no known biological effects of the inductive field Studies have shown that a field strength much higher than the Telemetry Research SmartPad have no effect The level of magnetic field is quite low and remains useable only 5 cm above the SmartPad Telemeter How many animals can be monitored at one time Each Telemeter sends its signal on one of 16 unique transmission channels Therefore up to 16 Telemeters can be used within a 5 m range of one another If you need to have more animals in close proximity contact Telemetry Research to discuss a customized solution ls there any interference between Telemeters No Each Telemeter uses a separate transmission frequency No shielding is required between cages Close proximity is possible including multiple Telemeters in one animal The transmission frequency is in the 2 4 GHz band What is the life expectancy of the Telemeters The Telemeters are designed for the implantation under the skin As this is a relatively hostile environment users should not expect the same life time as for be
30. ing that it is receiving power from the PC 9 Telemetry Research User Manual for Small Animal Systems v1 3 3 The PC will open the Found New Hardware Wizard window Choose the No not this time option and click Next Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CD or on the Windows Update Web site with your permission Read our privacy policy Can Windows connect to Windows Update to search for software Yes this time only Yes now and every time connect a device No not this time Click Next to continue lt Back Cancel 4 Click Next to install the driver automatically Found New Hardware Wizard This wizard helps you install software for TR190 Configurator If your hardware came with an installation CD lt or floppy disk insert it now What do you want the wizard to do Install the software automatically Recommended Install from a list or specific location Advanced Click Next to continue 5 Ignore the driver signing warning Configurator drivers are safe to install Click Continue Anyway Hardware Installation i AN The software you are installing for this hardware TR190 Configurator has not passed Windows Logo testing to verify its compatibility with Windows xP Tell me
31. ing signals all analog outputs are low pass filtered at 1 kHz Telemeter model Output Output2 Outputs Biopotential 1 Biopotential 2 eee Green Yellow Red Black TR50B Biopotentia TRE7Y The SmartPad Is designed to charge small animal Telemeters while they are implanted in the animal To do this place the animal cage on the SmartPad so that it is against the raised edges in the back left hand corner To protect the Telemeter battery the SmartPad automatically controls the strength of the charging field using information from the Telemeter It is important that Telemeters are only placed on SmartPads set to the same channel otherwise over or under charging may damage the battery The Charging field in the SmartPad is also set to cycle automatically and turns off for 30 minutes every two hours this however does not significantly affect recharging of the Telemeter battery The SmartPad charges the Telemeter using an electromagnetic field generated above and below the SmartPad For this reason it is important that a SmartPad is not placed on a metal surface or above or below other SmartPads unless 40 cm apart If the SmartPad is to be used on metal shelving for data collection and output only the charging field can be disabled using the Configurator System 13 Telemetry Research User Manual for Small Animal Systems v1 3 Example Multiple SmartPad Setup lf metal shelves are used while the charging field is enabled the SmartPad
32. meter C gt 3 7 art Search for 3 Battery Level Diagnostics Telemeter Current transmission channel of Telemeter 4 Battery Level acceptable 5 Charging onfigure your Telemeter 6 Pressure Sensor function Change transmission channel to if applicable 7 Potentiostat function if applicable 6 Click OK to confirm Cin enim Confirm configuration New Configuration Telemeter 5565 wil be in safe mode Confirm that you wish to place this Telemeter into safe mode To take the Telemeter out of this mode will require placement on a SmartPad 1 Please move the Telemeter 1 meter away from SmartPad 2 Please make sure the battery is charged up before storage Click OK to confirm 7 The Telemeter is now in Safe Mode Make sure the Telemeter does not come within 1m of any active SmartPad or the Telemeter may become Active again confgsoh E 28 Telemetry Research User Manual for Small Animal Systems v1 3 Positioning the Telemeter During Surgery Signal Maximization The location of the Telemeter will vary dependent on the size species of animal and the location of the signal being measured In small animals like rats and guinea pigs it is recommended that the Telemeter body be placed inside the abdominal cavity The pressure catheter can be inserted into the abdominal aorta for arterial pressure measurement and or electrodes can be tunnelled subcutaneously to the
33. nch top based laboratory items However with appropriate care during implantation and explantation users should expect to reuse the Telemeters many times SmartPad questions need to record from my animal while it is near metal How can I do this Use the Configurator System to disable the charging field of the SmartPad Remember that the Telemeter will run of battery power and therefore data can be collected for approximately 4 hours if fully charged Further questions Telemetry Research is proud to offer unlimited technical support and advice to all its customers Our team of engineers and physiologists can advise on specific applications and equipment configurations Email support telemetryresearch com or phone 64 9 367 7126 43 Telemetry Research User Manual for Small Animal Systems v1 3 Contact Information Telemetry Research Limited PO Box 5504 Auckland 1141 NEW ZEALAND TEL 649 367 7126 FAX 4649 367 7157 EMAIL info telemetryresearch com WEB www telemetryreseach com TELEMETRY RESEARCH 44 Telemetry Research User Manual for Small Animal Systems v1 3
34. nols iodophors and hypochlorite Please confirm with Telemetry Research before using any product other than the approved products listed High level Cidex Activated Advanced Sterilization Gluteraldehyde 1 2 hours j disinfectant Dialdehyde Products J amp J 25 C 77 F Solution Products J amp J 20 C 68 F 25 C 77 35 Telemetry Research User Manual for Small Animal Systems v1 3 Telemeter Diagnostics Using the Configurator System the user can investigate the status of the Telemeter To run diagnostics enter the serial and channel numbers of the Telemeter and click the Start diagnostics button on the Telemeter Configuration amp Diagnostics page The Telemeter can continue to transmit data while diagnostics is running Pressure Telemeter Oxygen Telemeter After clicking on the Start Diagnostics you will be asked if you want to save a log file This can be useful to monitor the Telemeter over a period of time or if you are asked to send information to support staff at Telemetry Research The log file and display are both updated every 10 s PLEASE NOTE Depending on the Telemeter you have the may appear if the telemeter does not have that function ie oxygen telemeters will have a for the pressure sensor Battery Level y the battery is more than 20 charged x the battery is low and the Telemeter needs to be recharged If the Telemeter is not placed on an active SmartPad it will
35. of the catheter allowing measurement of pressure signals directly where the tip is located The sensing tip should be handled carefully Never lift the i Telemeter by the tip pierce the tip with a i sharp object or hit the tip against a hard surface Temperature measurement All Telemeters record temperature When collecting temperature data from the Telemeter at the same time as charging temperature is updated once every two hours Continuous temperature measurement is available if the charging field is disabled 15 Telemetry Research User Manual for Small Animal Systems v1 3 Configurator Software Overview ConfigSoft can be started from either the e Desktop icon OR TR ConfigSoft e Start gt Programs gt Telemetry Research gt ConfigSoft h ay J Evernote for VAIO N ad FileZilla FTP Client amp Games JoeS J Intel J Intel PROSet Wireless Documents d iTunes d Maintenance Pictures _ Microsoft Office J Microsoft Silverlight J Mozilla Firefox I Mwsnap Computer do Norton Online Backup a a PMB Ontrol Pane dJi Protector Suite J Qlock d QuickTime J Skype d Startup Help and Support Ji Telemetry Research R ConfigSoft VideoLAN d VoipRaider Music Devices and Printers Default Programs 4 Back JE Getting Started Telemeter Configuration and Diagnostics 4A Se SmartPad Configuration and Diagnostics Help amp Support From the main menu there are fo
36. om the SmartPad corresponds to the following voltages 1V 0nA 2V 200 nA Calibrations coefficients will also be supplied with the electrodes which will need to be used in calculating the correct oxygen concentration Prior to shipping all Telemeters are calibrated so that the voltage analog output from the receiver corresponds to the following temperatures OV 0 1V 20 25 Telemetry Research User Manual for Small Animal Systems v1 3 Telemeter general use Before implanting a Telemeter make sure to write down the serial number and Channel setting All Telemeters are factory set to frequency Channel 1 and need to be activated and configured to different channels before use The Channel is set using the Configurator System SENSORS Telemeter Label example TRM54P 4168 Pa The Telemeter model TRM54P the Serial number is 4168 The serial number is also on the outside label of the Telemeter packaging Telemeters are designed to be recharged either before or inside the animal It is recommended to implant the Telemeter fully charged To charge the Telemeter before implantation place the Telemeter at the centre of a SmartPad configured to the same Channel Full charging may take 4 5 hours Use the diagnostics section of ConfigSoft to determine the battery level It is important that the Telemeter is implanted to lie within the base of the abdomen or under the skin of the abdomen to enable efficient recharging within t
37. ore construction and implantation Mesh fitted to Telemeter before sterilization e DO NOT AUTOCLAVE e Ethylene Oxide gas sterilization e Chemical Sterilization NOT ethanol Telemeter and mesh must be rinsed thoroughly with sterile saline See Sterilization section for more details of recommended sterilization procedures Removal At the end of the experiment great care must be taken when removing the Telemeter from the animal Tissue will grow into the mesh so it may be difficult to see the Telemeter body Never use a scalpel to cut away the mesh Scissors should be carefully used taking great care not to cut the Telemeter body catheter or electrodes The mesh pouch should be discarded and a new one fitted for the next use 30 Telemetry Research User Manual for Small Animal Systems v1 3 Surgical Mesh for BP Catheter Implantation Tissue adhesive can detach from tissue after a period of time allowing the BP catheter to pull out of the vessel To avoid this we recommend the use of a piece of surgical mesh at the time of implantation Scar tissue will grow into the mesh fabric holding the catheter in place and reducing reliance on the glue itself 1 Insert catheter into the vessel and use the smallest amount of glue to hold the catheter in place 2 Dry the surrounding area using swab sticks 3 Cut a small square of surgical mesh 5 x 5 mm and cut a small slit in the mesh 4 Place the mes
38. ot use alcohol as a sterilizing agent This will damage the Telemeter Tissue Oxygen Telemeters The electrodes in particular the carbon paste electrode should not be sterilized Please contact support telemetryresearch com for appropriate recommendations Disinfection Sterilization 1 The Telemeter must be cleaned rinsed and dried before disinfection Soil debris proteins and water can interfere with the effectiveness of the following procedure Note that some disinfectants have a limited usable life after activation or opening the container failure to take notice of the warnings can inhibit the effectiveness of the disinfection process Prepare the disinfectant according to the manufacturer s instructions Soak the Telemeter in the disinfectant at the temperature and time intervals listed Rinse the Telemeter well in sterile pyrogen free water or sterile saline O A O N At least three separate rinses are required Do not reuse any of the water used for rinsing since it will be contaminated with the disinfectant 34 Telemetry Research User Manual for Small Animal Systems v1 3 CAUTION Use only the listed cleaners and disinfectants for the times temperatures indicated CAUTION DO NOT sterilize by autoclaving radiation gamma or e beam plasma peroxide or formaldehyde vapour solutions Chemical Sterilants Available from most hospital supply companies chemical sterilants are considered cold sterilants and should be
39. ow are for the TRM5 series Telemeters Some specifications may not be relevant depending on the model of the Telemeter Pressure sensor accuracy 2 mmHg Pressure sensor drift lt 4 mmHg per month Pressure sensor Frequency response DC to 500 Hz 3 dB Standard pressure catheter Distal tip 660 um OD 2Fr Catheter 500 um 1 5Fr length dimensions 9cm Pressure sensor range 20 300 mmHg Ambient pressure range 650 to 800 mmHg 1300 m Biopotential Input range 2 mV Biopotential Resolution 12 bit A D TO Biopotential High pass characteristics Biopotential Electrode leads Biopotential Electrode diameter Low Pass filtering by SmartPad Cut off frequency 1000 Hz Maximum operating altitude Transmission range Up to 5 m range may vary depending on laboratory configuration Weight Approximately 12 5 gms Battery life TRM5 Telemeters are designed to operate continuously when operated with a SmartPad If the animal is away from the SmartPad charging field or the SmartPad charging field is turned off battery life is 4 hrs in Active Mode and approx 6 months in Safe Mode On off mechanism Activation via the SmartPad and deactivation via Configurator system Calibration No user intervention required calibration values stored within each Telemeter Pressure 1V output OmmHg 2V output 100 mmHg Analog output calibration values Biopotential 2V output OmV input 4V output 2 mV input Temperature OV output 0
40. p Diagnostics Help amp Support 1 Input senal number and transmission channel of your SmartPad Search for Smart Pad Start Di ti Diagnostics 1 Serial Number 2 Firmware Version 3 Charging Field 4 Atmospheric Pressure Sensor function 5 Temperature normal 6 Power consumption normal File F Help H Getting Started Telemeter Configuration amp Diagnostics SmartPad Configuration amp Diagnostics Help amp Support Material Links De mw y e O e X http www telemetryresearch com IMPLANT MONITOR DISCOVER Solutions Expertise About Us We provide innovative wireless systems for monitoring physiological signals in freely moving laboratory animals Contact Us TELEMETRY RESEARCH search Telemetry Research combines with Millar Instruments to provide high fidelity pressure monitoring via telemetry in small animals more SNA Temperature Combination Sensors Transmitters Receivers Charging Acquisition amp Analysis Latest News Setting up a TR Telemetry System TR releases training videos on YouTube Recent Visitors Eugene Nalivaiko University of Nevecastle Australis on Julian Patons tractor Telemetry Research User Manual for Small Animal Systems v1 3 Pairing a SmartPad and Telemeter Before implanting a Telemeter into an animal a SmartPad and Telemeter
41. r area with instruments such as forceps or tweezers e When handling the catheter with either fingertips or surgical instruments always handle the catheter 5 10 mm proximal to the sensor area The sensor area contains very fragile wires which may be damaged or broken if the catheter is gripped too close to the sensor or too tightly be GENTLE e Always know the location of the catheter tip e Do not place heavy objects or metal instruments on top of the catheter or Telemeter e Take care not to cut the catheter during surgery or when removing the Telemeter from the animal DOR DONOT Sensor Do NOT clean with high pressure water jet Protect sensor tip when not in use surface tip Do NOT expose to excessive pressure Catheter Clean immediately after use Do NOT cut crease knot fold kink or Body crush with forceps or clamps of any kind After removal Keep catheter and sensor wet Do NOT expose to alcohol cresols from animal until cleaned phenols mercury compounds hypochlorites acetone peroxide silicone chlorine xylenes trichloroethylene or freon Clean thoroughly with approved Do NOT use ultrasonic cleaner enzymatic cleanser immediately after use Disinfection Dry catheter before sterilizing with Do NOT autoclave irradiate or ethylene oxide gamma ebeam plasma peroxide or Sterilization formaldehyde vapour solutions Use a recommended cleaning Do NOT use Sporox or Cidex PA solutions agent 32 Telemetry Research
42. recording site Antenna Charging coil Charging Maximization For best charging performance the Telemeter must be implanted so that it is parallel with the charging pads As the antenna is on the opposite side of the Telemeter to the TR label it is recommended that the Telemeter be implanted so that the label is facing inwards as this will give the best possible range In rats the Telemeter should be placed within the abdominal cavity and attached to the muscle wall Biopotential lead separation Depending on the model Telemeters may be supplied with bipolar electrodes that are suited to ECG data collection The bipolar electrodes are supplied connected down their entire length While separation of the ends is required to allow appropriate placement we recommend that the electrodes be kept together and run alongside one another for as far as practical This will reduce electrical noise in recorded biopotential signals A free length of no more than 3 4 cm is recommended for each electrode It is not recommended that sutures be placed around the leads other than at the point of contact with the tissue as this may provide a stress point on the leads and cause them to break Example A SN placement of electrode leads ae for recording of i high quality ECG Y Attach to xiphoid process Leads kept together as far as practical 29 Telemetry Research User Manual for Small Animal Sy
43. rmware Version Input senal number and transmission channel of your Telemeter Serial number of Telemeter C gt D Start Seach for 3 Battery Level Current transmission channel of Telemeter 4 Battery Level acceptable 5 Charging ure your sjemete 6 Pressure Sensor function Change transmission channel C D if applicable 7 Potentiostat function if applicable 4 Enter the serial number of the Telemeter The number is on the outside of the Telemeter box or on the round label on the Telemeter under the model number Telemeter a serial number d i 5 Select or enter the current Channel number of the Telemeter first use Channel 1 6 To change the Channel number select or enter the new Channel number in Configure your Telemeter 7 Click Go to activate the new Channel New Configuration Telemeter 5565 will be changed from channel 1 to channel 3 Please move the Telemeter 1 meter away from SmartPad Click OK to confirm 8 Click OK to confirm the choice 19 Telemetry Research User Manual for Small Animal Systems v1 3 Telemeter s channel is now 3 yp This Telemeter can operate with a SmartPad on channel 3 9 A message confirming the new Telemeter Channel should appear To make it easier for future reference record the Telemeter Serial number and Channel number for all Telemeter configurations If using a SmartPad with a Channel
44. s or surgical equipment instruments The purpose of the detergent is to remove blood serum proteins and tissue debris from the surface of the Telemeter 33 Telemetry Research User Manual for Small Animal Systems v1 3 Terg A Zyme is an enzyme active powdered detergent made by Alconox Inc To make a 1 solution mix 10 grams of powder with 1 Liter of cold or warm water Allow the Telemeter to soak for a minimum of 15 minutes and a maximum of 24 hours in the solution Rinse thoroughly preferably with running water Fisher Scientific is a company that supplies Terg A Zyme catalog 50 821 785 www fishersci com but please refer to the Alconox website for other companies www alconox com Enzymatic Enzol in Advanced Propylene Glycol 15 mins room Detergent UK Sterilization temperature Cidezyme Products J amp J temperature Terg A Alconox Sodium 15 mins room Zyme Dodecylbenzene temperature Note If checking the offset calibration of the Telemeter the conditions listed under Calibrations Values must be replicated i e clean hydrated at 37 C and in the dark Sterilization Preventing infection is very important in collecting quality recordings of normal physiology and animal survival It is important to make sure the implanted Telemeter is sterile before implantation Under no circumstances should the Telemeter be autoclaved or subjected to temperatures over 60 C as this will damage the Telemeter Do n
45. stems v1 3 Construction of Mesh Pouches To allow the Telemeter to be securely held in place in the abdomen a mesh pouch can be fitted to the Telemeter Sutures through the mesh pouch can then be used during surgery to attach the Telemeter to the abdominal muscle layer Itis important that the Telemeter is not secured in place by suturing around either the catheter or electrode wires as this may lead to wire breakages The pouch should be made from surgical mesh or similar biocompatible material Silk or similar suture material should be used to hold the mesh in place rather than sewing cotton Suitable mesh is supplied as part of the BP Consumable Pack from Telemetry Research The piece of mesh supplied can be used to make 8 small animal pouches The excess mesh can be used for securing the blood pressure catheter to the vessel Use this piece for BP catheter implantation Construction 1 Cut a rectangle of mesh Small animal Telemeters 4 x 8 cm 2 Fold the mesh over the Telemeter body and secure using sutures at a few points around the Telemeter as pictured below Care must be taken not to pierce the silicone Telemeter body with the needle or to cut or damage the catheter or electrodes Sterilization Surgery Mesh not fitted to Telemeter construct pouch at the time of surgery e Autoclave mesh with surgical pack e Ethylene Oxide gas sterilization e Alcohol Chemical mesh must be rinsed thoroughly in sterile saline bef
46. stics SmartPad Configuration amp Diagnostics Help amp Suppor 1 Senal Number Serial number of SmatPad TRI80 Current transmission channel of SmatPad Smart Pad 2 Firmware Version l You will be asked if you want to O cron ts save a log file This can be useful P E 4 aiaa Prose to monitor the SmartPad over a n l PR period of time or if you are asked 2 Pier coneumpcn sre to send information to support Staff at Telemetry Research The log file and display are both updated every 10 s Press OK to continue coos O a E In diagnostics mode data sampling from the SmartPad is disabled and LED on SmartPad will flash The SmartPad will now be in Diagnostics mode and the Status light on the front will flash orange IFR ConfigSoft File F Help H _ Getting Started Telemeter Configuration amp Diagnostics SmartPad Configuration amp Diagnostics Help amp Support 1 Input senal number and transmissi Serial number of SmartPad TR180 5617 i 1 Serial Number t issi el P 1 Current transmission channel of SmartPad 2 Fir ns ewer 3 Charging Field 4 Atmospheric Pressure Sensor function 5 Temperature normal 6 Power consumption normal 38 Telemetry Research User Manual for Small Animal Systems v1 3 Atmospheric Pressure Sensor function v the atmospheric pressure sensor is functioning normally
47. ted within sterile packaging o Place the Telemeter on the SmartPad and plug the power cable into the SmartPad This will automatically switch the Telemeter into Active Mode Be aware that the Telemeter is now sending data and using battery life o If the SmartPad is configured to the same transmission channel as the Telemeter the Status light on the front of the SmartPad will be green Safe Mode Use Place on Configurator active System SmartPad Active Mode If monitoring is not taking place for a period longer than 7 days it is recommended that the Telemeters be placed in Safe Mode to protect battery life damage to the Telemeter can occur if left in a fully discharged state 27 Telemetry Research User Manual for Small Animal Systems v1 3 Placing a Telemeter into Safe Mode Connect Configurator to the computer with ConfigSoft loaded Start ConfigSoft Make sure the Telemeter is not within 1m of an active SmartPad Enter Telemeter Serial and Channel numbers Under Put Telemeter into Safe Mode Press Go an ite gt ConfigSoft ac Ehli X j File F Help H Sod Rese ase Telemeter Configuration amp Diagnostics SmartPad Configuration amp Diagnostics Help amp Support 1 Ensure your Telemeter is in Active Mode by placing Telemeter on SmartPad 1 Serial Number 2 Input senal number and transmission channel of your Telemeter 2 Firmware Version Serial number of Tele
48. the SmartPad is glowing either orange or green e Check that you entered the correct Serial and or Channel numbers e Move SmartPad closer to Configurator The Telemeter is now in Active Mode and sending data The battery life in this mode is approximately 4 hours for a fully charged unit If data collected is not required immediately we recommend you place the Telemeter into Safe mode using the Configurator System Repeat the above process for each of the SmartPads and Telemeters to configure each pair to a different Channel Your telemetry system is now configured and ready for use Charging field The Charging field option in the SmartPad Configuration and Diagnostics Dialog allows the user to enable turn on or disable turn off the charge field of the SmartPad By default when the SmartPad is powered the charge field will be turned on Turning the charge field off may be useful if recording from an animal while it is in an experimental chamber running wheel etc where the SmartPad will be near metal The Telemeter battery will not charge when placed on a SmartPad with the field disabled so data collection will only be possible for approximately 4 continuous hours The Telemeter will after this time need to be placed ona SmartPad with the field enabled for a minimum of 5 hours 22 Telemetry Research User Manual for Small Animal Systems v1 3 Trouble shooting and frequently asked questions What if do not know the Channel setting of t
49. the use of an enzymatic cleaner a protein film will form that can result in pressure signal drift 3 After soaking in the enzymatic cleaning solution thoroughly rinse the Telemeter with fresh distilled water or saline 4 After rinsing is complete gently dry the catheter as follows e Fold a soft tissue e Use gentle stroking to brush dry the catheter tip e Do not pull the catheter tip through a folded tissue e Do not allow the catheter to air dry on a tray table or countertop 5 After the cleaning and drying procedure is finished e Return the Telemeter to its original plastic the tray e Protect the sensor area on the catheter tip by carefully placing it in the foam that came with the Telemeter e Slide the tray inside the box and now store the Telemeter in a cool dry place until the next use CAUTION Delays in rinsing a Telemeter after removal will reduce cleaning effectiveness Examine the pressure sensor active surface diaphragm for blood or materials not removed by cleaning A dirty sensor may cause short term baseline drift when used the next time Cleaning Agents Tissue adhering to the catheter tips may be removed by enzymatic digestion The Telemeter can be soaked in a cleaning detergent such as Pyroneg An enzymatic detergent can help in removing biological tissue from the catheter e g Terg A Zyme These are generally available from most hospital supply companies they are generally labeled for use on fabric
50. ties any products returned for evaluation and or service must be approved in advance by TR Customer Service Repairs All products returned outside their warranty period have a USD 125 00 evaluation fee This evaluation fee and any additional costs will be deducted from any approved service repair work All Telemeters including sensors and electrodes are non repairable Returned products to Telemetry Research must include 1 A copy of the original invoice showing the date of shipment if under warranty 2 A Return Materials Authorization form 3 A signed Biosafety Clearance products must be clean Telemeters should be cleaned and sterilized disinfectant Failure may result in further charges incurred or the product returned to the customer Failure to comply with the required documentation will delay processing or may result in the products returned to the customer This publication may not be reproduced or transmitted in any form electronic or mechanical including photocopying recording storing in an information retrieval system or translating in whole or in part without the prior written consent of Telemetry Research Limited Telemetry Research Limited TEL 64 9 367 7126 PO Box 5504 FAX 64 9 367 7157 Auckland 1141 USA 415 287 3875 New Zealand EMAIL info telemetryresearch com Contents WME OCG WOM e a a seco ea a E E seeees esse ests 2 Telemetry SysteM COMPONE NMS cccceecccececeececeececececeuceseceseuc
51. ts of this manual may not apply to your system lf you have any questions please contact us at support telemetryresearch com Key Features Accuracy For long term high frequency physiological recordings Digital wireless recording of data with the highest quality signal integrity 24 7 Data can be collected continuously 24 hours a day while the animal is on the SmartPad with no need to replace batteries Versatility Telemeters send data wirelessly from within the animal to the SmartPad where it is converted to an analog signal compatible with data acquisition systems Low cost of ownership Battery replacement is not required With user care Telemeters can be re charged and re used many times Efficient housing Animal cages can be next to each other in one location for simultaneous data collection from multiple animals No specialized cages or shielding is required as each Telemeter operates using one of 16 independent frequencies with no interference to recordings service amp Support Telemetry Research prides itself on being responsive to our customers current and future needs We have experienced engineers and scientists on staff to provide professional expert and timely support and advice 2 Telemetry Research User Manual for Small Animal Systems v1 3 Telemetry System Components The Telemeter Care must be taken not to damage the Telemeter body catheter or electrodes Telemeters are not repairable Depen
52. ur areas to select from 1 Getting Started Quick Start Small Animal Telemetry System setup see page 4 2 Telemeter Configuration and Diagnostics 3 SmartPad Configuration and Diagnostics 4 Help amp Support 16 Telemetry Research User Manual for Small Animal Systems v1 3 Telemeter Configuration and Diagnostics File F Help H Telemeter Configuration amp Diagnostics Getting Started SmartPad Configuration amp Diagnostics Help amp Support on SmartPad Serial number of Telemeter Current transmission channe of Telemeter Configure your Telemeter Change transmission channel to 1 Ensure your Telemeter is in Active Mode by placing Telemeter 2 Input senal number and transmission channel of your Telemeter Put Telemeter into Safe Mode a Diagnostics 1 Serial Number 2 Firmware Version 3 Battery Level 4 Battery Level acceptable 5 Charging 6 Pressure Sensor function if applicable 7 Potentiostat function if applicable SmartPad Configuration and Diagnostics Help amp Support 17 UR ConfigSof File F Help H Getting Started _Telemeter Configuration amp Diagnostics Serial number of SmartPad TR180 Current transmission channel of SmartPad 2 Configure your SmartPad V Change transmission channel to F Charging field SmartPad Configuration am
53. why this testing is important Continuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Continue Anyway STOF Installation 10 Telemetry Research User Manual for Small Animal Systems v1 3 6 The driver may take a few moments to install Please wait while the wizard installs the software S F TR190 Configurator e w dfCoinstaller01009 dll To C AWINDOWS system32 lt Back Next gt Cancel 7 If successful the Status light on the front of the Configurator should now be green indicating that it is communicating with the PC Click Finish to close the Window Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for TR190 Configurator Click Finish to close the wizard Tee cancel 8 The Configurator System is now ready for use with Telemeters and SmartPads 11 Telemetry Research User Manual for Small Animal Systems v1 3 SmartPad Setup The SmartPad is a receiver and charger in one device Do not operate a SmartPad near a metal surface unless the charging field has been disabled using the Configurator System All SmartPads are shipped set to Channel
54. x a problem has been detected with the atmospheric pressure sensor This can be caused by a brief loss of communication with the SmartPad If it occurs frequently or continues for more than 30s please contact Telemetry Research for assistance Support telemetryresearch com Temperature normal v the temperature of the SmartPad is at acceptable levels x the temperature is above acceptable limits Please contact Telemetry Research Support telemetryresearch com Power consumption normal v SmartPad power consumption is normal x Excess power consumption has been detected The Status light on the front of SmartPad should be red This may be because the SmartPad is too close to a metal surface or another SmartPad The SmartPad will continue receiving and outputting data but the charging field will have been disabled To correct the situation try moving the SmartPad away from any metal surfaces and other SmartPads and unplug and plug in the power cable to reset the SmartPad If the problem continues please contact Telemetry Research for assistance Support telemetryresearch com SmartPad Diagnostics Log files The log file from SmartPad Diagnostics is likely to be useful only to Telemetry Research Staff It contains the following list parameters followed by some further information about the SmartPad status Elapsed Atmospheric Current Time SmartPad Pressure Temperature Draw Charging Time second ID mmHg AP Raw C T Raw mA CD

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