ADAM SCC User Manual
Contents
1. a Saved experiment 10 Data list Information Digital Bio Figi Main Frame of ADAM Report Program si Digital Bio 24 Section 6 ADAM Report Program Guide 6 2 ADAM Report Program Function Guide Image Auto Save OFF Image path Data list Information Digital Bio D Image frame Image captured by ADAM will be shown here Function Buttons Start cell counting saving images exporting data and all function of Report Program are handled by using these buttons see p 24 for more detailed information of each button 3 Data List All saved data in ADAM will be loaded and shown in data list section Information Operation and counting results of each frame will be displayed here Graph Analysis of results including cell size and frame by frame counting will be shown in graph section PO Sy Digital Bio 25 ADAM Report Program Guide Function Buttons Start cell counting Park Lock stage of ADAM Eject chip holder out of ADAM Loads the experiment data from the memory of the main device Deletes all of the loaded data and memory of the main device Transfers the data list to Excel format and saves it Auto Save Turn on or off automatic image save option OFF Default image save folder is C Program Files Digital Bio ADAM Images Images will be saved until your hard drive has no more capacity to save Be sure to set Auto Save off unless you need to save image2. 2 2 Identification of System Components Fig 1 View of the ADAM Control Button Door External video monitor LCD 1 Control buttons A Eject Ejects the chip holder from the Adam gt Run Start Performs all procedures of automatic counting Parking Protects the alignment of stage from external shock when the ADAM is moved to the other places It is strongly recommended to park ADAM before turning tt off 2 Door Chip holder comes out here 3 LCD Displays the process and the result 4 Keypad Inputs the sample number and Enter button Less than 3 characters 5 External video monitor To see the actual cell shape and check if any clumped cell through this monitor H 1 Digital Bio 1 Section 2 Product Description 2 3 Identification of System Components continued Fig 2 Rear view of the ADAM Fan Power Switch Key Pad Port Power Plug RS232C Port N USB Port 7 7 m Video Port Fan Adam s cooling fan Power switch Main power on off control Power plug Connect the ADAM power cord to wall outlet Video Port External video monitor port USB port Connect the USB serial cable to computer RS 232C serial port Not Connected Port for only QC and Service Key pad port Connect the Keypad NJ OY U1 B WN j 1 Digital Bio 12 Section 3 System Installation 3 1 Environmental Requirements To insure correct operation and stable performance over an ex
3. 2 64 i coumt 3 6 Ali count 4 92 Ali count 5 69 Ali count 6 x Digital Bio a ADAM Report Program Guide Graph NONVIABLE 1 NONVIABLE 2 lt i Digital Bio This section shows information of cell size distribution and counting results of each frame that ADAM captured Through cell size graph you can figure out whether there are cell clumps or aggregates In case of counting evenly distributed cells without any aggregation there should be a single peak on distribution of cell size The size of cell in graph is not real size of cell It is the size of pixels in fluorescence image captured by ADAM And the size information is only to judge if there is a lot of aggregated cells P Digital Bio ASA Trouble shooting Troubleshooting Table Problem ADAM does not power up Inaccurate result Software does not work When error message is shown For information on each error message see page 20 j 1 Digital Bio Cause Power switch in off position No power from outlet Bad power cord Cell number may be out of range SCC kit solution has expired PC setup incorrect wrong instruct mode Cable s not fully connected wrong adaptor When there are too many frames with errors Error message Check sample When too many cells are loaded Error message Over Range Solution Check power switch on back of unit Check power sourc
4. cell concentration in the N channel of the chip Store kit box upright and at room temperature Expiration date of stain solution is written on the bottom of the kit box yy mm dd Be sure to check the expiration date before using Follow the exact steps detailed in the Instructions for Use section Add 100 ul of the raw milk sample in the tube Mix the sample thoroughly by turning the vial upside down 3 5 times Load 20 ul of the cell sample onto the Chip Ensure that no bubbles enter the channel j 1 Digital Bio Section 2 Product Description 2 1 Packing List The ADAM is shipped with the following components Once you receive your instrument please check that all items listed below were shipped If any items are missing or damaged contact your local distributor or sales digital bio com Item Quantity Main device 1 Somatic cell counting SCC Kit CRS K01 Instruction Manual External video monitor Optional Installation CD Key Pad Power Cord USB cable Fuse N e e e e he rme re After receiving ADAM examine it carefully for any damage incurred during transit Any damage claims must be filed with the carrier AN CAUTION Neglecting to remove any or all shipping brackets or foams prior to operation may result in damage to the equipment The shipping brackets or foam inserts must be reinstalled prior to shipping the unit to prevent damage to the equipment H 1 Digital Bio 10 Section 2 Product Description
5. detection technologies to make the cell analysis more accurate and reliable To count cells using ADAM the cells are mixed with a Propidium Iodide PI stain and directly pipetted on to a disposable plastic chip The chip is then loaded onto a precision stage An ADAM system is automatically focused onto the chip and cells that have been stained are recorded by a sensitive CCD camera The image results are automatically processed generating the cell count which is displayed on the front of the instrument Simple Fast Accurate Reliable C N Concave Mirror lt aa SSS s C mh Excitation filter Lenses N Green LED 4ch Chip Objective lens Dichroic filter Emission filter Mirror S CCD camera la o si Digital Bio Section 1 Introduction 1 2 Technology Measurement Section SCC Kit CRS KO1 is composed of Propidium iodide PI for counting somatic cells CRS KO1 can be used without diluting raw milk Measuring range of cell density is O to 1 x 107 cells ml One kit is capable of 100 tests Each tube has 100 ul reagent of somatic stain solution Simply add the same volume of the raw milk sample in the tube then every preparing for experiment end Once the experiment is complete the results can be printed through the thermal print Printed number indicates cell concentration x 1000 ml in each channel T The cell concentration in the T channel of the chip N The
6. may see Check Sample or Over Range message at display monitor If you see those messages please check below list first O Digital Bio 19 General Operation lt In case of Check sample gt 1 Check sample contamination or Chip with dust or other materials 2 Check mixture of sample and agent It has to be mixed well 3 Check test tip whether stained with something If you have problems that mentioned above you will get a result sheet like below ee Count SCC 000ML ooiz 2010 0323 ooii 2010 03 23 ooid 2010 0323 ooog 2010 03 23 Ooo 7201003 of ooo Ol o 0 3 woe 000E 2010 03 2 wee jga 201003 T D004 2010 03 2 IP Po Bo po a 5 Cf e S vaa Oty en ey ey EREEREER ENEA ed ee a lt In case of Over Range gt 1 In case of the result of cell counting is over 4000 X1000mL 2 Check mixture of sample and agent It has to be mixed well If you have problems that mentioned above you will get a result sheet like below _Semioer_ Dato ___ __Time __ _Cow __ Count SCCMOOOimL Chip ooi2 2010 0323 ooii 2010 0323 oo1i0d FOLD soO3 23 00ga 2010 03 23 OoOoe YOl Oo E ay oe a LN ooo YO Oo E af te OoO06 2010 03 ote jds E ee 0004 2010 03 2 0003 2010 03 2 D Oo 0 a Te ay a pel eared og ee Ie te ee i La j Digital Bio 20 Section 4 General Operation 4 5 Maintenance and Cleaning ADAM does not ne
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8. ADAM SCC A New Standard of Automatic Cell Counter INSTRUCTION MANUAL j s Digital Bio Developed and Manufactured by NanoEnTek Inc E Mail sales digital bio com Webpage http www digital bio com NanoEnTek Inc HQ 12F Ace High end Tower 235 2 Guro 3 Dong Guro gu Seoul 152 711 Korea Tel 82 2 6220 7728 Fax 82 2 6220 7721 NanoEnTek Inc USA amp Europe 300 Washington St STE 416 Newton MA 02458 Tel 1 617 965 1414 Fax 1 617 965 1415 Copyright 2008 by NanoEnTek Inc All rights reserved Published in Korea j a Digital Bio All the materials in this manual are protected by Korean and international copyright laws They cannot be reproduced translated published or distributed without the permission of the copyright owner ADAM SCC User s Manual Homepage www digital bio com E mail Sales digital bio com Manufactured by NanoEnTek Inc 12F Ace High end Tower 235 2 Guro 3dong Guro gu Seoul 152 711 Korea Tel 82 2 6220 7911 Fax 82 2 6220 7721 E Mail sales digital bio com The information in this manual is described as correctly as possible and is applicable to the latest firmware and software versions but it may be changed without prior consent or notification Copyright 2008 by NanoEnTek Inc All rights reserved Published in Korea Documentation NESMU ASC 001E V 1 0 Revision History V 0 0 Date Oct 2008 V 1 0 Date May 2010 H 1 Dig
9. are Installation 23 Section6 ADAM Report Program Guide 6 1 ADAM Report Program Introduction 24 6 2 ADAM Report Program Function Guide 25 Appendix A Trouble Shooting 29 Appendix B Warranty 30 Appendix C Technical Specifications 31 Contact Information nn enn n nnn nn nnn nnn e nance 32 j 1 Digital Bio Introduction 1 1 Technology Mechanical Until now cell counting and viability measurement for many types of cells have been performed manually using hemocytometer with Trypan Blue exclusion method which is to distinguish viable cells from non viable cells One drawback of this method however is the propensity for the staining of artifacts another drawback is that the naked eye can only differentiate between cells in a limited concentration range in the hemocytometer chamber This combined with the potential problem of cell aggregation and limited sample volume leads to the common variation of counts normally associated with this method To address these problems Digital Bio has developed the ADAM which is based on a fluorescent microscopy technique for counting cells The ADAM utilizes sensitive fluorescence dye staining LED optics and CCD
10. e Replace Check the expired data Try again after vortexing the cells Check program setup Check all connections Check sample contamination or Chip with dust or other materials Check mixture of sample and agent It has to be mixed well Check test tip whether stained with something In case of the result of cell counting is over 4000 X1000mL Check mixture of sample and agent It has to be mixed well 29 Appendix B Warranty Digital Bio warrants that the ADAM will be free from defects in material and workmanship for a period of one 1 year from date of purchase If any defects occur in the ADAM during this warranty period Digital Bio will repair or replace the defective parts at its discretion without charge The following defects however are specifically excluded 1 2 NO U1 B W Defects caused by improper operation Repair or modification done by anyone other than Digital Bio or an authorized agent Damage caused by substituting alternative parts Use of fittings or spare parts supplied by anyone other than Digital Bio Damage caused by accident or misuse Damage caused by disaster Corrosion caused by improper solvent or sample For your protection items being returned must be insured against possible damage or loss Digital Bio cannot be responsible for damage incurred during shipment of a repair instrument It is recommend that you save t
11. ed regular maintenance ADAM has no replacement of consumable materials Clean the exposed outer surface of ADAM using a soft cloth and isopropyl alcohol or deionizes water AN CAUTION Dispose of wipes in an appropriately labelled solvent contaminated waste container si Digital Bio z Section 5 Hardware amp Software Installation 5 1 Connection between ADAM and computer The following steps will guide you to connect USB cable 1 Connect the USB cable to ADAM 2 Connect the USB cable to Desktop or Laptop computer 3 Turn on ADAM and Desktop computer Key pad port s a A i si Digital Bio 22 Hardware amp Software Installation 5 2 ADAM Report Software Installation To install the Adam Report software follow the directions as below 1 Insert the installation CD ROM into the computer Then open the file Setup_ADAM_v1 x x x exe Report program can be installed in Windows 2000 XP or higher version The start up dialogue of the software as shown below will appear Click Next to start installation 2 If you want to change installation folder click Browse and choose the location that you want After choosing installation folder click Install to proceed with the installation The computer activates the Installation of the Software w nitial installation folder is C Program Files Digital Bio ADAM 3 Report Program will be installed automatically 4 Click O
12. he Original packing material in which the instrument was shipped This warranty should be limited to the replacement of defective products For any inquiry or request for repair service contact sales digital bio com or your local distributor H 1 Digital Bio 30 AS ea e Technical Specifications si Digital Bio ADAM Voltage AC100 240 V 50 60 Hz Current max 1 8 A max 100 W Fuse F3 15AL250V Objective lens 4 X LED 4W Green LED IEC 60825 1 1993 A1 1997 A2 2001 CCD camera B W CCD Filter Excitation filter Dichroic filter Emission filter Weight 9 Kg Size WxLxH 220 x 375 x 250 mm Degree of protection IPXO AccuChip Measuring range 0 to 1x 107 cells mL Analysis time 2 2 5 min test Loading sample vol 20 uL for AccuChip 2 12 uL for AccuChip 4 test Measuring vol 8 5 uL for AccuChip 2 3 uL for AccuChip 4 test Somatic cell counting SCC Kit CRS KO1 Accessories Power cord 1 5 m Fuse 250 VAC 3 A F3 15AL250V Keypad External video monitor optional Environment Condition 5 lt T lt 30 Cc Altitude lt 2000 m 31 Contact Information E Mail sales digital bio com Internet www digital bio com NanoEnTek Inc HQ 12F Ace High end Tower 235 2 Guro 3 Dong Guro gu Seoul 152 711 Korea Tel 82 2 6220 7728 Fax 82 2 6220 7721 NanoEnTek Inc USA amp Europe 300 Washington St STE 416 Newton MA 02458 Tel 1 617 965 1414 Fax 1 617 965
13. ied manner is strongly discouraged and may result in damage or injury as cautioned by signed warnings j si Digital Bio Safety Symbols The following symbols are found on the instrument and this document Study the meaning of the symbols and always use the equipment in the safest possible manner Caution amp Warning Protective earth Ground C 4 This instrument and consumables conforms to the Declaration of Conformity Caution Biohazard Protective measures must be used in dealing with biologically hazardous materials such as carcinogenic reagents j si Digital Bio Item Battery inside device Cover Manual Sample handling Waste Operator j 1 Digital Bio Warning Risk of explosion if battery is replaced by an incorrect type This battery is not replaceable by a user Refer to a qualified personnel Do not remove a cover or dissemble a case There is no adjustable components inside the instrument If malfunction is found refer to a service personnel Do not attempt to service the equipment unless this manual has been consulted and is understood This manual is available in English only Failure to heed this warning may result in injury to service provider operator from electric shock mechanical or other hazards Wear gloves during sampling User s sample may have the infectious biohazardous substance After using Accuchips appropriately dispose it as biohazardous waste D
14. ital Bio Safety Precautions 1 Always ensure that the power supply input voltage match the voltage available in your location For operation environment See Appendix C Technical Specification This machine is air cooled so its surfaces become hot during operation When installing it leave a spaces of more than 10 cm 4 inches around it 4 Never insert metallic objects into the air vents of the instrument as this could result in electrical shock personal injury and equipment damage 5 Always set the main switch on the power supply unit to OFF before connecting the power cord to the wall outlet 6 Always ensure that the grounding terminal of the instrument and that of the wall outlet are properly connected The power cord should be connected to a grounded 3 conductor power outlet 7 TO avoid potential shock hazard make sure that the power cord is properly grounded 8 Do not position the equipment so that it is difficult to operate the disconnecting device 9 Be sure to set the main switch to OFF unplug the power cord and lock the stage before moving 10 If the instrument is broken or dropped disconnect the cord and contact a authorized service person Do not disassemble the instrument 11 Use only authorized accessories 12 Use this equipment only as specified in this manual and as specified in any documentation associated with its components Any use of the equipment in an unspecif
15. k to finish the installation If the installation was successful the report program can be found at Start gt All Program gt ADAM Installation of ADAM Operation Software 1 0 0 0 beta Instal ADAM Operation Sofiware vi 000 Deta Installation of ADAM Operation Software 1 0 0 0 beta a x This will install ADAM Operation Software v1 0 0 0 beta on your computer itis recommended that you exit all other programs before proceed with installation Click lt Next gt to continue installation Or click lt Cancel gt to cancel installation Copyright c Digital Bio Technology 2008 H si Digital Bio 23 ADAM Report Program Guide 6 1 ADAM Report Program Introduction This Report Program is designed to manage and report all results from ADAM All measurement results are automatically saved on the memory of ADAM The user can download the data from the memory of ADAM and export it to Excel xls format The user can delete data from memory of ADAM or can save captured images into Desktop or Laptop hard drive The data list window consists of the sample number chip date time total nonviable viable viability counting result in CAUTION Before running the program check the connection of USB cable between the Adam and the laptop or desktop computer Image Read data Auto Save Delete data OFF Export Excel Image path CW Temp ii NNDARRRERER KAD a Pa ats ellis aia Dyin Sita niga cite as a
16. ng process will be skipped 5 After calculating the cell number the chip will be ejected automatically Then chip can be removed 6 The calculated cell number per 1ml will be displayed automatically 7 For another experiment repeat the process from steps 1 5 8 Operator should remember the above procedure to perform the automatic cell counting with ADAM 1 Mix sample with SCC Kit CRS K01 amp loading the sample 2 Channel Chip 20 pl 4 Channel Chip 12 pl 2 Inserting of the chip Simple amp Easy Re total cats oe SICK FESHI 3 ste p S S 2x10 mil J H 1 Digital Bio 16 Section 4 General Operation 4 3 Menu setting You can set the menu as you press the button on the keypad from the screen for inputting cow numbers n 12 00 Configuration Chip selection gt gt Input cow number Dead pixel erase LCD contrast System info You can find the setting modes by selecting the number from the Menu 4 3 1 Chip Selection User can select the 2 kinds of chip type One is the two channel chip AccuChip2 Another is the four channel chip AccuChip amp Configuration Chip Selection 1 2 Channel chip 2 4 channel chip Chip selection Dead pixel erase LCD contrast System info Choice 1 Chip Selection 1 2 Channel chip 2 4 channel chip Value is rerecorded Press ior 2 and Enter Choice 1 After pressi
17. ng the Enter key the screen will return to the Menu screen automatically H 1 Digital Bio 17 General Operation 4 3 2 LCD contrast Press the number 4 key to adjust the brightness of the LCD screen from the MENU Configuration o Configuration amp Chip selection Chip selection Dead pixel erase Dead pixel erase LCD contrast LCD contrast System info System info LCD Contrast Value 730 Value is rerecorded Range 600 750 Adjust LCD contrast if the letters do not appear clearly on the screen Press the Enter key after you input the three digits The range is from 600 to 750 After pressing the Enter key the screen will return to the Menu screen automatically 4 3 3 System Information The device versions and date which have been installed in the device can appear when the number 5 key is selected from the MENU After pressing the Enter key the screen will return to the Menu screen automatically amp Configuration System Info Chip selection 1 Version SCC V1 00 Dead pixel erase 2 Date Jun 22 09 LCD contrast CORE1 1 52 System info COCE2 1 50 OL 0 gt 4 IC eae lee H 1 Digital Bio 18 Section 4 General Operation r Press the key after menu setting Once inputted the screen will return to the counting mode automatically 4 4 Result Analysis If the density of sample is over range you
18. o not reuse the Accuchips Must have the general knowledge of cell counting procedure and bio safety to handle the sample that may have the infectious biohazardous substance Date Aug 01 2008 Aug 01 2008 Aug 01 2008 Aug 01 2008 Aug 01 2008 Aug 01 2008 Table of Contents Page Section 1 Introduction i t Technology Mechanical s2 9sssseresareesenaeaaesesneseen sesh eneseseeee l 2 Technology Measurement Section 77 7 7s7enmsnhnimeneinanii 9 Section2 Product Description 2 1 Packing List 10 2 2 Identification of System Components view of the ADAM 11 2 3 Identification of System Components rear view of the ADAM 12 Section3 System Installation 3 1 Environmental Requirements 13 3 2 Power on and Initial Display 13 3 3 ICON FUNCUONS 74 4iaiaaa eases aer Sapa se esses eoeeeea esas ase 14 Section4 General Operation d Material S Method s2sss sssesssesasesessesssa a 15 4 2 COUNLING TOtal Cell 7is sssa sia 16 4 3 Men Setting sas ossseaqnorases a a a a 17 da ReCuilAllaly sic aiee ree es ase aaressss sear eae ieeeereis 19 4 5 Maintenance and Cleaning 21 Section5 Hardware amp Software Installation 5 1 Connection between ADAM and computer 4 22 5 2 ADAM Report Softw
19. ormance such as x1000 mL eef eject run parking or insert 123 24 Status of the Chip Holder 45 586 789 135 2 channel or 4 channel chip Features Shows when cell counting is running After you press the run button Shows the Chip Holder is ejected After you press the eject button Shows the Chip Holder is parked After you press the park button Shows the Chip Holder is inserted Shows that ADAM reads 4 Channel chip i si Digital Bio 14 General Operation 4 1 Material amp Method The following list contains the items needed for somatic cell counting using the Adam A raw milk sample SCC Kit Pipette and tips 1 The prepared materials ready to be used Raw Milk 3 Mix the sample by turning the vial upside down 3 5 times 4 Load the cell sample onto the Chip Ensure that no bubbles enter the channel 12 pl ae Ve Ni D Mt Eg N2 AccuChip AccuChip H 1 Digital Bio 15 Section 4 General Operation 4 2 Operating the ADAM 1 Press EJECT button on the main device to eject the chip holder 2 Insert the Chip loaded with the sample onto the chip holder Please be careful not to make bubbles 3 Press the P Run button on the main device 4 Automatic Focus will be carried out at the first time the device is booted Once ADAM have done the Auto Focus process and on the following time focusi
20. s Example of saved image file 081221 yymmdd 203482 hhmmss N1 channel name 002 bmp Choose folder to save images automatically H 1 Digital Bio 26 ADAM Report Program Guide 3 Data List 0 ee 20100323 71 50 31 a il Foiofoss23 21 50 31 oo0id FoOiadfoss 23 21 50 31 OoOn03 7O10 os aw Ps Zs 71 50 31 000g z0 0i ol ay ee 03 51 56 ooo0Ff 2010 03 2 03 51 56 j0 2010 03 24 000s FOLOs 03 2 0004 ANE ooo 2010 03 2 0002 2010 03 2 oo01 7oio 03 Jr m Oo m i _ Fa cn oo a m N i in w D E C909 09 9 9g 9 oa D a O oa A TAE e e eE E E eee le eels eels Da 6 fo Di ee pai N E R i O i ee D rh Data list shows data stored in ADAM memory Total amount of stored results are indicated at bottom of list as Saved experiment Up to 1000 counting results are automatically saved to ADAM memory When memory of ADAM is full new counting result will replace old data These data can be exported as Excel Sheet xls and stored in personal computer or can be erased from ADAM memory 4 Information ion This section shows information regarding Ti count De operation of ADAM If cell counting is ce started through Report Program the Ti count 18 75 counting results of each frame that ADAM ENN E captures will be shown here Ti count 21 7 Ti count 22 72 Ti uto Count 1030504 T1 error frame 0 Ti Total 1604 MI counk 1 95 Ali count
21. tended period of time install the ADAM in a location which meets the following conditions e Room temperature between 20 and 35 C Not recommended for cold room use 4 C CAUTION At low temperature 10C please warming up the system for 10 min e Not exposed to direct sun light e Not subject to direct or continuous vibration e Not subject to intense magnetic or electromagnetic fields e Relative humidity between 0 95 e Area free from corrosive gases or other corrosive substances e Area with very little dust or other airborne particles e Allow a 10 cm minimum space around the instrument for proper air flow e Not allow to put heavy material on top of ADAM 3 2 Power on and Initial Display 1 Check the connection of the main device power cord 2 Make sure that the main power switch is in the ON position On the rear side of the main device When you turn on the ADAM it will go through self diagnostic tests including all optical components If a problem is detected please contact your local distributor or sales digital bio com If boot up is successful and no errors are detected the home screens will be displayed as below c 12 00 ADAM SCC i Digital Bio gt gt Input cow number Soot aT i si Digital Bio 13 System Installation 3 3 Icon Functions Ap a E 12 00 ID aie Ni 678 40 T2 N2 gt System time Display a status of the perf
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