User Manual - Cyagen Biosciences
Contents
1. determine the viable cell density IMPIOO79A1 GUXNX 07021 Page 2 of 4 ccyagen 2 Centrifuge the cells for 3 5 minutes at 250 x g and 20 C Remove and discard the Supernatant using a pipette 3 Resuspend the cell pellet in the OriCell Neural Stem Cells NCR Protein Free Cryopreservation Medium at a cell density of 10 10 cells mL 4 Dispense aliquots of the cell suspension into cryogenic storage vials that are properly labeled 5 Place the vials directly in a 80 C freezer After 24 hours transfer the frozen vials to liquid nitrogen for long term preservation Thawing 1 Remove the cryovial of frozen cells from storage and quickly thaw the vial in a 37 C water bath 2 Gently dilute and resuspend the cells in 8 10mL of cell culture medium 3 Centrifuge the cells for 3 5 minutes at 250 x g and 20 C 4 Carefully aspirate off as much of the supernatant as possible and add 2 3mL of cell culture medium to resuspend the cells 5 Transfer the cells into an appropriate growth vessel with the appropriate amount of growth medium and incubate inside a 37 C incubator with a 5 CO humidified atmosphere STABILITY AND STORAGE Store at 2 8 C This product is stable at 2 8 C for up to 3 years and should be discarded beyond the labeled expiration date For optimal performance repeated warming and freeze thawing should be avoided QUALITY CONTROL OriCell Neural Stem Cells NCR noncontrolled rate Protein Free Cr2. ee k Yd U e n We help You discover life OriCell Neural Stem Cell NCR Protein Free Cryopreservation Medium Cat No GUXNX 07021 _ m a am ai of I e re O cyagen PRODUCT DESCRIPTION OriCell Neural Stem Cell NCR noncontrolled rate Protein Free Cryopreservation Medium is a serum free and ready to use freezing medium for NSCs Its chemically defined and protein free formulation has been optimized to NSCs thus greatly enhancing the viability and integrity of these cells by protecting them from damage during the one step freeze thaw procedure Unlike other conventional freezing media which require a slow programmed freeze our product allows the cells to be resuspended and put directly at 80 C This product is intended for laboratory research use only It is not intended for diagnostic therapeutic clinical household or any other applications FEATURES e Design for Neural Stem Cells e Stable and ready to use e Chemically defined and protein free e Promotes high cell viability gt 70 for NSCs post thaw e Maintain stem cell pluripotency e Does not affect the proliferation and differentiation properties of cells e Cells can be directly frozen at 80 C thus eliminating the need for a programmed freeze PACKAGING Available in two sizes 50 mL Bottle 20 mL Bottle INSTRUCTIONS Cryopreservation 1 Collect cells that are in the logarithmic growth phase Perform a cell count to
3. yopreservation Medium has been tested for performance on mesenchymal stem cells and Vero cells The standard evaluation includes e Sterility test bacteria fungi and mycoplasma e pH test e Osmolality IMPIOO79A1 GUXNX 07021 Page 3 of 4 O cyagen e Endotoxin Cyagen Biosciences reserves all rights on the technical documents of its OriCell cell culture products No part of this document may be reproduced or adapted for other purposes without written permission from Cyagen Biosciences IMPIO079A1 GUXNX 07021 Page 4 of 4
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