H301-MINI / Issued
Contents
1. Figure 6 shows how to position the ring Ring selection guide diagrams rings for 35 and 60 mm dish Figure 7 and Figure 8 re spectively www oko lab com pee H301 MINI User Manual November 2014 Figure 6 1 Insertion of the ring 2 insertion of the 35 mm dish 3 insertion of the magnetic lock NOTE Spacing rings are included with sample holder Grainer Petri35x10 Ibidi p Dish35mm low BD Falcon 35x10 Willco 35x10 Corning 35x10mm MatTek P35G X 14 X Figure 7 Ring selection scheme for 35 mm dish 2 Ibidi p dish 50mm low MatTek P50G X 30 X CO BD Falcon 60x15 Grainer Petri 60x15 Corning 60x15mm Q Willco60x15 Figure 8 Ring selection scheme for 60 mm dish jojo www oko lab com s H301 MINI User Manual November 2014 6 Working with 1x3 and 1x2 chamber slides magnetic locks Magnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder Figure 9 Magnetic lock for 1 x 3 chamber slide Figure 10 Magnetic lock for 1 x 2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 www oko lab com ew H301 MINI User Manual November 2014 7 Connection of the Gas Supply A single silicon tubing carries output gas from the Okolab Gas Controller to the H301 MINI Silicon tubing connects to a gas input brass2. opening located on a corner of the H301 MINI See Figure 11 Connect by gently pushing silicon tub ing onto brass opening Figure 11 Connection with gas supply 8 Working with Perfusion The Chamber H301 MINI features 12 perfusion holes on the sides for the insertion of perfusion tubing up to 2 5 mm in outer diameter Small screws plug the perfusion holes when not in use screws plug M3x0 5 THD Remove small screws plug as necessary before introducing perfusion tubing Figure 12 shows location of perfusion holes T Sensor Perfusion Tubiry Input Pons Perfusion dedicated opening 1 Frontal view 2 3D view Figure 12 Perfusion www oko lab com paged H301 MINI User Manual November 2014 9 Connection of the Chamber with XY stage H301 MINI fits on the XY Stage without any screws To fix the chamber on the stage follow the steps shown in Figure 13 1 Place the chamber on the stage See Image 1 of Figure 13 2 Incline the chamber and slide it in direction of the springs until it snaps into the housing See Image 2 of Figure 13 3 Image 3 of Figure 13 shows the chamber fitted on the XY Stage Figure 13 Connection of the Chamber with XY Stage www oko lab com Page9
3. H301 MINI User Manual November 2014 Compatible with the following XY stages Compatible with the following Okolab Controllers Physik Instrumente P 737 1SL 2SL 5SL H301 T BL PLUS e Ludl 99A600 UNO COMBINED CONTROLLER It fits in any MW plate holder H401 T DUAL www oko lab com Page 1 H301 MINI User Manual November 2014 Index le CONBDOMEMUS and CIMMEMSIOINS 6455 stocnscoce eens cccauecenecoacvecc tonctanc seis ocaseeetencaaueceascoeetececencmscacian senses te pm 08 2 Co 2 2 cae yer eee Pane nen Pee Py PnP ray TE Pe A ne ee ee ne AAT 2 1 Available Sample a 6 0 6s ts em as ms rs Sree Onan ad One a One ae ene re ner ee 3 Insertion of the Sample Feedback Temperature Sensor cccccccccccccccccccccccccecccecccecccesccesccescceccees 4 Insertion of Sample Holder into CHaMDGEN biciscssss 050000000se0e seen suse sees sneceeeeseawsawseenseerbessseansuesseusseeeees 5 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic LOCKS ceccceecccceccccsccceeccceeccs 6 Working with 1x3 and 1x2 chamber slides Magnetic lOCKS ccecccccccccccccccecccccccecccecceecceeccess T COMMECHION OF ENE Gas SUDDIV fice co cues cs coca E nuacsuaceusacueuesiqoeacuneceuseeseseacceusousecoueoee Be WORKING WICH EEr TO sccceac ecco ccs rescence eee as cearteee cea ueecceacseecsenscecsaectueseuecenaseeeneneecnaceeamas 9 Connection of the Chamber with XY stage ccccccccccc
4. cccccccccecccccccecccecccecccecccecccecceeccseccseccseccess www oko lab com es H301 MINI User Manual November 2014 1 Components and dimensions H301 MINI includes the following components Chamber main body with embedded temperature sensor Heated glass lid with Indium Tin Oxide ITO conductive coating and embedded temperature sensor Distance between focal plane a and upper side of the glass gt N lid 127 70 Sample Holder Order Separately wy Chamber top view gt pe Chamber Base Figure 1 H301 MINI Components and Dimensions www oko lab com pape H301 MINI User Manual November 2014 2 Sample Holders 2 1 Available Sample Holders The following sample holders are available NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list 1 35mm Petri dish 1 1x3in chamber slide 1 Lab Tek 1in x2in chambered cover glass 1 Lab Tek II 1in x2in chambered cover glass 1 60mm Petri dish 1x35 M single 1x60 M single 1xGS M single For 1 35mm Petri dish holder For 1 60mm Petri dish holder For 1 1 x3 chamber slide holders 1xLABTEK M single 1xLABTEK II M single For 1 1 x2 Labtek For 1 1 x3 chamber slide and chambered cover glass holder 2 35mm petri dishes Figure 2 Available sample holders 3 Insertion of the Sample Feedback Temperature Sensor Insert
5. the Sample Feedback Temperature Sensor through the dedicated opening located in the H301 MINI see Figure 3 Frontal and 3D views Temperature Sensor dedicated opening Perfusion Tubing Input Ports 1 Frontal view 2 3D view Figure 3 Insertion of the temperature sensor inside the chamber www oko lab com Paget User Manual November 2014 H301 MINI 4 Insertion of Sample Holder into Chamber _Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold er To introduce a sample holder with the proper orientation match the red dot on the holder to the one on the _chamber base as illustrated in Figure 4 a Buis W Sox a H301 MINI Insert the adapter by superimposing the red points Figure 4 Introduction of the Specimen Holder inside the Chamber Base 5 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks _ Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder Figure 5 illustrates the available magnetic locks for 35 and 60 mm dishes Threaded magnetic posts allow adjusting Magnetic Locks for 35 and 60 mm petri dishes Included in the corresponding sample holder holder s height Figure 5 Magnetic locks for 35 and 60 mm dish NOTE Magnetic locks are included with sample holder Spacing rings accounting for variability in dish diameter from one manufacturer to another are also included
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