Eksigent Software Drivers
Contents
1. 42 Installation Guide Eksigent Software Drivers 3 of 42 Contents eksigent part of AB SCIEX Eksigent Software Drivers Installation Guide 4 of 42 The Analyst Software Installation Software Installation This section describes the procedures required to install the Eksigent control software on the host computer The computer should already have the Analyst software installed and configured for use Tip If you intend to move an installed Eksigent LC device to a new computer follow the steps in Appendix A to transfer the instrument configuration calibration and method settings to the new computer before installation a Tip If your operating system is Windows XP SP1 or SP2 refer to Appendix A before starting the Analyst software 1 Insert the Eksigent software installation CD or download the software installation files from www Eksigent com setup D Tip Any existing versions of the software do not need to be removed F ie Setup Eksigent Welcome to the Eksigent Setup Wizard This will install Eksigent 3 11 0 on your computer It is recommended that you close all other applications before continuing Click Next to continue or Cancel to exit Setup i Cancel Figure 1 1 Eksigent Setup dialog lt lt lt Note If the Setup Wizard dialog does not appear run the file setup exe from the CD 2 Click Next to continue through the installatio2. 17 Mass Spectrometer Configuration using the HyStar Software 17 Device status 20 Device method editors 21 Additionalinstrtumentdialogs 23 Data acguisilon iii ston stances II Oe OG ae eS WAA 24 Chapter 3 The Xcalibur Software Installation 25 Software Installation 25 Driver configuration 26 Configure the LC system 27 Mass Spectrometer configuration using the Xcalibur software 27 Device StalUS onc AI AA IKAWA AI Hee oS UA 31 Device method editors 32 Additionalinstrtumentdialogs 34 Peak parking 35 Data GCOUISIWION lt 6 b on24 6 sau Opweneee tegedenedes sheet eds bees aes 37 Appendix A Configuration Files Transfer 39 Configuration files 39 Appendix B Known Issues 00 0 eee es 41 The Analyst SONNIG UWA oe eo ene ee sere eee ceeu el torses lt 41 systems with Windows XP SP1 41 systems with Windows XP SP2 41 TheHyStarSoftware
3. 25 C Autosampler Idle Figure 1 11 The Analyst Software Application Status Dialog Eksigent Software Drivers 12 of 42 Installation Guide The Analyst Software Installation Build acquisition methods An acquisition method in the Analyst software combines the methods of each individual device 1 Open anew acquisition method by clicking the Build Acquisition Method icon on the Analyst Software Navigation Bar All configured devices are present 2 Acquire 1 IDA Method Wizard E Build Acquisition Method Build Acquisition Batch zo Express View Figure 1 12 The Analyst Software Navigation Bar 2 Select the Eksigent device to assign a method and then select an existing method on disk using the button Acquisition method Software Application Properties Pe Method Path C Projects ManoLl_206_PreBeta settings method Mass Spec 0 000 min col min Filename gradientini By Eksigent Device CHI By Eksigent 451 Figure 1 13 The Analyst software acquisition method editor Eksigent device methods for LC systems can be created and edited using the LC Method button in the Eksigent software dialog See the Eksigent Software User Manual for more information on creating methods Acquisition method Software Application Properties EMI Acquisition Method Path C Projects NanoLC_206_PreBeta settings EKAS1 p Mass Spec 0 000 min T ol min Filename Am Aa Fy Eksigent Device CH1 By Eksigen
4. 000 pL min Runtime 00 02 21 00 05 00 vial Weil AL Loft Inj Vol 0 0 Oo Store data in ra Ss AA_01_30 d Chromatogram Additional Signals Handling of the additional signals from LC system Color Show Signal source HB LC Pump 1 Pressure l E LC Pomp 2 Pressure O LE Column Pressure a mz E LC Po Fow Deselect all E O i Soient moe oe oe os ny Figure 2 12 Eksigent data displayed on HyStar real time page and being stored in HyStar data file All Eksigent systems provide four auxiliary data channels to the HyStar software Flow A Qa flow B Qb the raw A D signal and Pressure at column Pc Flow units are nl min Pressure is in psi and the A D signal is volts Eksigent systems equipped with UV and PDA detectors will provide both the chromatogram and the spectral data to HyStar The units are AU Absorbance units Eksigent Software Drivers Installation Guide 24 of 42 February 2012 The Kcalibur Software Installation Software Installation This section describes the procedures reguired to install the Eksigent control software on the host computer The computer should already have the Xcalibur software installed and configured for use Tip If you intend to move an installed Eksigent LC device to a new computer follow the steps outlined in Appendix A to transfer the instrument configuration calibration and method settings to the new computer before installation 1 Insert the eksi
5. File name Express 100 hs Places Files of type Hardware Setup file hss exthss Cancel 2 Figure 2 5 Open Harware Setup File Dialog Installation Guide Eksigent Software Drivers 17 of 42 The HyStar Software Installation Scroll down to LC System and select the box next to it In the box in the lower part of the dialog select Eksigent LC System Click Settings from the LC System Setup group SS D e Configure Eksigent may become disabled that is if the Eksigent system is equipped with a Tip Depending on the selected configuration several HyStar selections column heater the Oven item will become disabled and set to Controlled by x General System System Times amp Yolumes Accessory Settings List of Hardware Setup files Look in Hardware categories Hardware Port Interface Ic Hystar Contigs By Solvent Delivery System System Eksigent LC System v Ep Sample Introduction Demo ALS WLAN express 00woven hss 4 Separation Device Oven 4 nanole hss AR LC Detectors Eksigent LC System Eksigent LC System System System LC System Eksigent LC System COM1 UR Interface fia NMR Spectrometer fi Mass Spectrometer C MN Fraction Collector G AUK Ausiliary devices Valve devices LC System Setup r S Choose the LC System and define connecting p
6. NoneManual Duration 60 min Detection External Detector Auxillary A D channel available Figure 3 14 Eksigent LC Device Control Dialogs Eksigent Software Drivers Installation Guide 34 of 42 The Xcalibur Software Installation Peak parking Peak Parking is a method that extends the analysis time of MS MS interrogation for peaks of interest eluting from the LC column The LC column flow rate is immediately reduced to a predetermined rate when the initial portion of the peak fraction enters the MS The Xcalibur software can control the built in peak parking feature of Eksigent LC devices This feature is controlled within the Xcalibur software MS method by custom Data Dependence criteria See the The Xcalibur Software Reference Guide for more information about these parameters and their meanings Custom Data Dependence W Enabled Actions Activation threshold 100000 Divert valve Deactivation hysteresis High voltage Contact closure Dependence mode le Base peak 1 Mass list a Figure 3 15 MS acquisition data dependence settings During sample acquisition the Eksigent Device Status window will indicate the current state of the LC device when peak parking is in progress The Eksigent Software Acquisition window will also display all relevant device information when peak parking is in progress ga Note Peak parking is controlled via external trigger The instruments must be connected toge
7. purpose and assumes no responsibility or contingent liability including indirect or consequential damages for any use to which the purchaser may put the equipment described herein or for any adverse circumstances arising therefrom For research use only Not for use in diagnostic procedures The trademarks mentioned herein are the property of AB Sciex Pte Ltd or their respective owners Eksigent is a division of AB Sciex LLC AB SCIEX is being used under license Eksigent 5875 Arnold Road Dublin CA 94568 AB Sciex LP is ISO 9001 registered 2012 AB SCIEX Printed in Canada REGISTERED COMPANY Contents Chapter 1 The Analyst Software Installation 5 Software Installation 5 Driver configuration 6 Configure the LC system 8 Create hardware profiles using the Analyst Software 8 View instrument status 12 Build acquisition methods 13 Peak DaIKING 2c 60 ote eee ca Gente che baton Bee aA baw hE ewe ehebes aha 13 Chapter 2 The HyStar Software Installation 15 Software Installation 15 Driver configuration AA ee eee ee 16 Configure the LC system
8. 60 280 Qa b Pa Pb Pe B channel 7 300 310 320 330 340 350 Acquiring miz Sequence C calibur methodsexpr 00 sld Sample Name MAU PDA o a P Run Manager NL 7 30E1 a Working On Seq Row 1 100 Tatu TICF MS data0i 060125124311 Position 1 JE a AAA 3 5 4 0 45 5 0 gt NL 6 63 fl 5 Channel A UY data01_060125124311 al Fake MS gt k Running 0 0 05 1 0 15 2 0 2 5 3 0 3 5 4 0 45 5 0 Time min T 245 00 a 238 00 A a 214 00 225 00 __ I rE ii TISA AAA 300 320 340 360 330 wavelength nm NL 1 31E7 a Total Scan PDA data01_060125124311 00 Os 1 0 1 5 2 0 25 3 0 35 40 45 5 0 Time min m NL 4 56E4 H z AID Card Ch 1 AD card data01 060125124311 13 9 7 104721993870 100 100 T Col o Power 294 4 C 00 05 10 15 20 25 30 35 40 45 50 Time min Running Runtime 00 00 40 00 03 00 A 266 B 73 4 a NL 3 21E4 E A S TOF amp A D Card Ch 14 AD card 2 data01_06012512 Timed Acquisition Now Beginning Ep 0 0 0 5 1 0 1 5 2 0 2 5 3 0 3 5 4 0 Time min 0 0 0 5 1 0 1 5 2 0 2 5 3 0 3 5 4 0 Time min 0 0 0 5 1 0 1 5 2 0 2 5 3 0 3 5 4 0 Time min 4 5 5 0 fa NL 2 50E3 J Ri AJD Card Ch 1 AD card 3 data01 0560125124 0 KIA o gt 4 5 5 0 NL 5 91E3 i a AD Card Ch 1 AB card 4 data01_060125124317T 0 4 5 5 0 NUM 1 25 2006 12 44 PM Z Figure 3 17 Eksigent data displayed on Xcalibur real time page and being stored in Xc
9. Configuration The Xcalibur software is now configured to control the installed devices Eksigent Software Drivers 30 of 42 Installation Guide The Xcalibur Software Installation Device status The status of all installed devices can be viewed at any time by selecting the device from the Status tab in the Main Xcalibur Roadmap dialog 2101 x File Actions View Tools GoTo Help pmu 2 Q sigent LC Channel 2 Ready to Download i ke MS a I Qa Pb Pe A B channel Fake Qb P A B 48 6 32 1 1047 2217 5913 00 00 uLimin psi Power Waiting for LC Method Runtime 00 00 00 00 00 00 40 BO Figure 3 9 Channel 1 status The Device Status Window will indicate the current state of the installed instruments and additional instrument specific information such as mobile phase flow rates column pressures and temperatures Eksigent LC Channel 2 Ready to Download Se Eksigent NanoLl 457 Autosampler Not Connected Statue 10 Not Connected Temp O C Plate 48 vials 4 20 2005 4 17 PM Figure 3 10 NanoLC AS1 Autosampler status 7 Note For multi channel devices the status information is specific to the selected A channel lt lt lt Note If there is a problem with the device an error condition will be indicated in the _ al status window Installation Guide Eksigent Software Drivers 31 of 42 The Xcalibur Software Installation Device method edit
10. Parking Acquisition method Enabled EM Acquisition Method Ee Mass Spec 0 000 min Ae Period 0 000 min oe Ba HEPI IDA Criteria Percentage of total flow Minimum number of candidates to find lon source gas 1 G51 lon spray voltage 15 Figure 1 16 The Analyst Software MS Acquisition Method Editor Note If the Peak Parking criteria are set too low in the IDA method the analysis may never end and will require manual termination Rey ja During the sample sequence the Analyst Software Application Status Dialog indicates the current state of the LC device when peak parking is in progress The Eksigent software acquisition dialog also shows all relevant device information when peak parking is in progress Eksigent Software Drivers Installation Guide 14 of 42 The HyStar Software Installation Software Installation This section describes the procedures required to install the Eksigent control software on the host computer The computer should already have HyStar 3 2 installed and configured for use Tip If you intend to move an installed Eksigent LC device to a new computer follow the steps in Appendix A to transfer the instrument configuration calibration and method settings to the new computer before installation 1 Insert the Eksigent software installation CD or download the software installation files from www Eksigent com setup D Tip Any existing versions of the s
11. alibur Raw data file All Eksigent systems provide four auxiliary data channels to the Xcalibur software Flow A Qa flow B Qb the raw A D signal and Pressure at column Pc Flow units are nl min Pressure is in psi and the A D signal is volts These signals are labeled by the Xcalibur software A D card 1 ch 1 A D card 2 ch 1 A D card 3 ch 1 A D card 4 ch 1 respectively Eksigent systems equipped with UV and PDA detectors can provide both the chromatogram and the spectral data to the Xcalibur software The units are Absorbance Units AU The data passed to the Xcalibur software can be optionally enabled disabled to save both display and storage space by selecting Instrument Setup gt Eksigent LC Channel 1 or 2 Menu gt Data Collection This affects both real time display and storage in a raw file A restart of Installation Guide Eksigent Software Drivers 37 of 42 The Xcalibur Software Installation the Xcalibur software is required These settings are per channel for 2 D systems and are stored as a user preference NanoLC data channels for Xcalibur xj M Collect ausiliany data pressure flows IY Flow amp Gal I Flow B Obj IY 4 0 signal W Column Pressure Pc IY Collect detector data chromatogram spectral W Chromatogram IY Spectra Please restart calibur for the changes to take effect Cancel Figure 3 18 Configure desired amount of data collection Eksigent Software Drive
12. ation C Program Files Eksigent HanoLC calibur Mot Registered Analyst TF1 5 Eksigent System Driver Registered 451 Autosampler Driver Mot Registered 452 Autosampler Driver Mot Registered Method Merger Tool Registered Hy Star Mot Registered Calibration Disk Apply Figure 1 3 Eksigent Driver Configuration Utility Installation Guide Eksigent Software Drivers 7 of 42 The Analyst Software Installation Configure the LC system Refer to the Eksigent Software User Guide for setting up and calibrating your mass spectrometer The mass spectrometer must be configured properly prior to attempting to establish control within the Analyst software Create hardware profiles using the Analyst Software Caution If you are using Windows XP you must follow the directions in Appendix B prior to continuing Failure to do so will prevent the software and hareware from functioning properly If the drivers were installed correctly the Eksigent devices will be shown in the Companion Software area of the Analyst software Navigation Bar Analyst E Im fx File Edit View Tools Explore Window Script Help as S d a e a a st Explore Mode 28 5600 CTC fh BS e s e D ja ia All Gis On Oe APO OE Ap le Ti a levee eX joe oR GS ETRE et diel Manual Tuning 4 Acquire N Method Wizard E Build Acquisition Method ci Build Acquisition Batch Explore Ge Open Data File 8 Open Compound Database G Eksigen
13. devices can be viewed at any time in the top section of the Acquisition dialog in the HyStar software KA Hy Star Acquisition test4 xml 7 oj x Eile Module Acquisition View Options Compass Window Help l x not ready LC System Lamp Temperature Steblizing Off Gx 0b Pa Sana Status ready Tt Total Flowrate 5 000 pL min 1 VialfInj 1 1ofi A Runtime 00 00 00 01 00 00 sha Time ei 3 0 amp 497 B 503 Inj Vol 5 0 ul T Col Line ya Postion Sano 1D Method Store data in J Sample Table l Sample amp Reload 4 unkn test 3min m 4 Edit unkn_1 41_01_148 d M ihe J H a amp A z Pause Dont Zoom Cursor Stretch FF DA ltl Additional Signals velenath 280nm UY Chromatogram J Wavelength 280 0 0 144 From detector JEksigent LC System hystar For Help press F1 Sergei WA Figure 2 8 Eksigent Device Status dialog The Eksigent Device Status dialog will indicate the current state of the installed instruments and additional instrument specific information such as mobile phase flow rates column pressures and temperatures 6S Note For multi channel devices the status information is specific to the selected mai channel and are displayed side by side Note If there is a problem with the device an error condition will be indicated in the a status window Eksigent Software Drivers Installation G
14. eksigent part of AB SCIEK Eksigent Software Drivers Analyst Software 1 5 1 1 5 2 1 6 TF 1 5 1 HyStar 3 2 Xcalibur 1 3 1 4 2 0 2 1 2 2 Installation Guide D5031161 A February 2012 This document is provided to customers who have purchased AB Sciex equipment to use in the operation of such AB Sciex equipment This document is copyright protected and any reproduction of this document or any part of this document is strictly prohibited except as AB Sciex may authorize in writing Software that may be described in this document is furnished under a license agreement It is against the law to copy modify or distribute the software on any medium except as specifically allowed in the license agreement Furthermore the license agreement may prohibit the software from being disassembled reverse engineered or decompiled for any purpose Portions of this document may make reference to other manufacturers and or their products which may contain parts whose names are registered as trademarks and or function as trademarks of their respective owners Any such use is intended only to designate those manufacturers products as supplied by AB Sciex for incorporation into its equipment and does not imply any right and or license to use or permit others to use such manufacturers and or their product names as trademarks AB Sciex makes no warranties or representations as to the fitness of this equipment for any particular
15. gent software installation CD or download the software installation files from www eksigent com setup D Tip Any existing versions of the software do not need to be removed LF ie Setup Eksigent Welcome to the Eksigent Setup Wizard This will install Eksigent 3 11 0 on your computer It is recommended that you close all other applications before continuing Click Mext to continue or Cancel to exit Setup eksigent part of AB LUE i Cancel Figure 3 1 Installation screen lt lt lt Note If the Setup Wizard dialog does not appear run the file setup exe from the CD 2 Click Next to continue through the installation process Installation Guide Eksigent Software Drivers 25 of 42 The Xcalibur Software Installation gt gt Tip It is recommend to use the default directory specified in the installation dialog when prompted SF Driver configuration After the Eksigent software is configured for the instrument type and proper operation is verified configure the Xcalibur software drivers 1 Select Start gt Programs gt Eksigent gt Driver Configuration see Figure 3 2 The Driver configuration utility will automatically detect the version of Xcalibur software is installed on your computer Help F SDK 23 gt Batch Processing Driver Configuration e Eksigent age Firmware Update Lik Peak Viewer if Uninstall gf validate Installed Files Figure 3 2 Eksigent programs
16. he HyStar Software There is a known issue in the HyStar software that occurs when the CTC PAL autosampler encounters a hardware error such as a missing sample vial or misalignment There are two paths for recovery depending on whether data acquisition has begun 1 Before data acquisition begins The HyStar software will not allow data acquisition to be terminated because the system is not yet in a run state The HyStar button remains blue Right click the red autosampler button located in the HyStar Acquisition dialog Select Abort Injection and confirm the selection The HyStar button will change from blue to yellow or green Right click the red autosampler button again Select Reset Communication and confirm selection The autosampler communication will reset and the autosampler button will turn yellow and then green Once the hardware error condition is corrected the sample table can be reloaded and the analysis restarted 2 After data acquisition begins The HyStar software will not hang up however the autosampler communication will need to be reset by doing the following Right click the red autosampler button in the HyStar In the Acquisition dialog select Reset Communication and Confirm Selection The autosampler communication will reset and the autosampler button will turn yellow and then green Once the hardware error condition is corrected the sample table can be reloaded and the analysis restarted Eksige
17. ilable Import Export Figure 3 12 Eksigent LC Device Method Editor Select the device icon in the left side pane to edit the method for each device Refer to the Eksigent Software User Guide for more information about editing method parameters for each device 3 Untitled Thermo Xcalibur Instrument Setup File Eksigent NanoLC 451 452 Autosampler Help dicta 8 x eksigent 4 Eksigent LC 1 Eksigent NanoLC 4514AS Export Autosampler Method Test on B1 Figure 3 13 Autosampler Method Editor 7 Note For multi channel instruments each channel is considered an independent ji A device Installation Guide Eksigent Software Drivers 33 of 42 The Xcalibur Software Installation Additional instrument dialogs Access to the primary Eksigent Control software is provided through the drop down menu within the Instrument Setup dialog The main LC acquisition window as well as individual toolboxes diagnostics and control dialogs can be viewed here FB Untitled Thermo Xcalibur Instrument Setup File uungu Help Configuration Diagnostics Peak Parking Mobile Phase Direct Control Main Window hod Identification Data Collection Method ID defaut AA Column Information Manufacturer Eksigent particle size 3 pm Type ChromxP diameter 150 am Serial Number length 140 cm Eksigent Sample Injection Flow Profile NanoLCl 451 4S mary Run Conditions Gradient Profile Gradient Table
18. menu Check Eksigent System driver and the AS1 Autosampler options under the Xcalibur group see Figure 3 3 Click Apply If successful the status next to the check box will change from Not Registered to Registered ED Eksigent Driver Configuration Utility software Version 3 11 0 Build 120104 Location C Program Files Eksigent HanoL t xcalibur 2 1 Eksigent System Driver Registered AST SAS Autosampler Driver Registered Analyst Mot Registered Hy Star Mot Registered Calibration Disk Apply Figure 3 3 Eksigent driver configuration utility Eksigent Software Drivers Installation Guide 26 of 42 4 Click OK The Xcalibur Software Installation Tip If necessary you can later remove the driver from the Xcalibur software by using this utility checking the relevant boxes and clicking Apply Configure the LC system See the Eksigent Software User Guide for setting up and calibrating your mass spectrometer The mass spectrometer must be configured properly prior to attempting to establish control within the Xcalibur software Mass Spectrometer configuration using the Xcalibur software 1 Start the Xcalibur software Instrument Configuration Activate Windows Set Program Access and Defaults Windows Catalog Windows Update Accessories Programs ami Eksigent Figure 3 4 Instrument configuration shortcut Installation Guide E a akae 1 NA A February 2012 n Game
19. n process Installation Guide Eksigent Software Drivers 5 of 42 The Analyst Software Installation gt Tip Itis recommend to use the default directory and settings specified in the installation dialog when prompted F Driver configuration After the Eksigent software is configured for instrument type and proper operation is verified configure the Analyst software drivers 1 Select Start gt Programs gt Eksigent gt Driver Configuration see Figure 1 2 The Driver configuration utility will automatically detect the version of the Analyst software installed on your computer 7 Note Analyst software versions 1 4 1 or earlier are not supported Help SDK 23 gt Batch Processing 3 Driver Configuration e Eksigent age Firmware Update uk Peak Viewer if Uninstall af validate Installed Files Figure 1 2 Driver Configuration menu shortcut 2 Check Driver and the AS1 AS2 Autosampler options under the Analyst group see Figure 1 3 3 Click Apply If successful the status next to the check box will change from Not Registered to Registered Eksigent Software Drivers Installation Guide 6 of 42 The Analyst Software Installation 4 Click OK gt Tip If necessary you can later remove the driver from the Analyst software by using this utility checking the relevant boxes and clicking Apply Eksigent Driver Configuration Utility Software Version 3 11 0 Build 120104 Loc
20. nt Software Drivers Installation Guide 42 of 42
21. oftware do not need to be removed F is Setup Eksigent Welcome to the Eksigent Setup Wizard This will install Eksigent 3 11 0 on your computer It is recommended that you close all other applications before continuing Click Mext to continue or Cancel to exit Setup oxmgernt j Cancel Figure 2 1 Eksigent Setup dialog lt lt lt Note Ifthe Setup Wizard dialog does not appear run the file setup exe from the CD 2 Click Next to continue through the installation process Tip It is recommend to use the default directory specified in the installation dialog when prompted Installation Guide Eksigent Software Drivers 15 of 42 The HyStar Software Installation Driver configuration After the Eksigent software is configured for the instrument type and proper operation is verified configure the HyStar software drivers r Help SDK 23 gt Batch Processing Driver Configuration r e Eksigent age Firmware Update uk Peak Viewer i Uninstall af validate Installed Files Figure 2 2 Eksigent programs menu 1 Select Start gt Programs gt Eksigent gt Driver Configuration see Figure 2 2 The Driver configuration utility will automatically detect the version of the HyStar software installed on your computer Check Eksigent System driver option under the HyStar group see Figure 2 3 Click Apply If successful the status next to the check box will change from Not Registered to Regi
22. ons Simulate device Alias name Enable debug messages Cancel Help Figure 1 7 The Analyst software devices list 10 Repeat step 7 to step 9 to add as many devices as required _ Tip For NanoLC 2D systems add both Eksigent Device CH1 and Eksigent Device CH 2 For a NanoLC 1D add AS1 autosampler For QTRAP select all three devices in the hardware profile 11 Click Activate Profile see Figure 1 8 Hardware Configuration Editor Hardware Profiles MaseSpecOnly New Profile Mass Spectrometer 0 on GPIB Board 1 Edit Profile Software Application Eksigent Device CH1 0 Software Application Eksigent 457 1 Delete Braile Activate Profile Available Devices Help Figure 1 8 The Analyst software activated hardware profile The Eksigent Control software will start and if configured a utility dialog for the AS1 autosampler opens These dialogs will be visible during your experiments and will allow you to monitor the devices and edit methods 12 Click Close Eksigent Software Drivers Installation Guide 10 of 42 The Analyst Software Installation gt Tip Verify that the instruments are being controlled through the Analyst _ i software by checking the hardware status see View instrument status on page 12 Note For Eksigent Express 100 systems you may also want to add a PAL autosampler or a NI 6032E PCI A D converter Refer to the Analyst Software User Guide for mo
23. ors Methods for each device created within the Xcalibur software is similar to other instruments controlled by the Xcalibur software Ae Roadmap Home Page A ioj x File Actions View Tools GoTo Help G Run Manager es Ready To Download i Sequence Sample Name Working Orr Position os Inst Method Eksigent LC Channel 1 ks Ready to Download 5i Eksigent LC Channel 2 eee AI Setup Sege Setup ResulisRe view Qa b Pa Pb Pe channel TUIE Po DEE ae ae o 0 ower Waiting for LO Method Runtime 00 00 00 x 00 00 00 4 0 BO Browser For Help press F1 Mum iijesjeoo6 fizs39pm og Figure 3 11 Starting the Eksigent LC Device Method Editor To create or access a set of device methods select Instrument Setup in the Main Xcalibur Roadmap dialog Eksigent Software Drivers 32 of 42 Installation Guide The Xcalibur Software Installation 29 Untitled Thermo Xcalibur Instrument Setup File EksigentLC 1 Help KENA Summary Run Conditions Gradient Profile Gradient Table eksigent ge Method Identification 4 Method ID defaut Eksigent LC 1 Column Information Manufacturer lEksigent particle size 3 wm Type ChromxP diameter 150 am Serial Number length 140 cm Eksigent Sample Injection Flow Profile NanoLC 451 45 E NoneManual Duration 60 min Detection External Detector Auxillary AD channel ava
24. ort Solvents LC System JEksigent LC System z Connected via RS 232 Settings for LC Pump a Additional Description h ysta r 5 2 Hardware Setup Active Hardware Setup file C Hystar Configs E press100 hss Browse Open Saveds Print Close Figure 2 6 Hardware Setup Dialog 7 Select Solvent Delivery System In the lower part of the dialog select LC Pump 2 and choose Eksigent Pump 2 The Eksigent LC System selected earlier will be the channel 1 in this configuration Eksigent Software Drivers Installation Guide 18 of 42 The HyStar Software Installation 8 Select the device in the Eksigent Device drop down list i e NanoLC 2D Parallel Express 100 etc from the System Configuration group Eksigent Nano LC Aa x System Device 1 0 Advanced System Configuration Eksigent Device NanoL c 20 COM port cont Injection Yalye None System shut down if idle more than 120 min Figure 2 7 Eksigent Device Configuration 9 Set any other desired device configuration parameters 10 Click OK 11 After configuring the Eksigent devices click Close from the HyStar Hardware Setup dialog to save the configured device values to a configuration file The HyStar software is now configured to control the installed devices Installation Guide Eksigent Software Drivers 19 of 42 The HyStar Software Installation Device status The status of all installed
25. re information about these devices Device Type Autosampler kui Devices AutoS ampler CTC FAL E AutoS ampler Gilson 215 E Autosampler Gilson 233 E AutoS ampler Agilent 1100 E AutoS ampler Famos amp AutoS ampler PE200 E AutoS ampler Endurance Figure 1 9 The Analyst Software Autosampler Devices Installation Guide Eksigent Software Drivers 11 of 42 The Analyst Software Installation View instrument status To verify the status of each instrument controlled by the Analyst software click the window icon in the task bar at the lower right corner of the Analyst software dialog Ci4nalyst Data g Idle Fal Idle Figure 1 10 The Analyst software task bar The Software Application Status Dialog indicates the current state of the installed instruments and additional instrument specific information such as mobile phase flow rates column pressures and temperatures Software Application Status Ioj x h Brief Status Name Status Eksigent Device CH2 O lde Eksigent Device CHI Idle Eksigent ASI Pde Detailed Status Eksigent Device CH Actual Values Da QO nami Qb 1 nami Pe O p i uses NanoLlC Status 1 Waiting for Method Est Time Remaining 00 00 00 Eksigent Device CH1 Actual Values Ger 220 nm Gb 4 nL mir Pe 0 psi uses NanoLlC Status 1 Waiting for Method Est Time Remaining 00 00 00 Eksigent ASI A517 Autosampler Status Tray Temperature
26. rs 38 of 42 Installation Guide Configuration Files Transfer Configuration files After moving an installed Eksigent LC device to a new computer to transfer the configuration calibration and method information to the new computer before attempting to use the device in its new location follow these steps 1 On your old computer run the Eksigent software access the Service Configuration dialog double click the status bar graphic and enter password techno Click Export Settings Close the software Copy the entire C Program Files EksigentNanoLC Settings directory to a transfer disk Copy the entire directory contents into the same folder location on the new computer create the C Program Files EksigentNanoLC settings directory Open the file C Program Files EksigentNanoLC settings EKsettings reg You will be prompted to copy the settings into the Windows registry Click OK Install the Eksigent software on the new computer Installation Guide Eksigent Software Drivers 39 of 42 Configuration Files Transfer eksigent part of AB SCIEX Eksigent Software Drivers Installation Guide 40 of 42 Known Issues The Analyst Software A significant issue has been identified for users using the Analyst software on Windows XP This issue prevents external device control on SP1 and causes the system to stop responding on SP2 Below are steps required to correct the issues using Analyst software and sof
27. s ao Et Documents H fan Startup m serie A 8 Internet Explorer le Sh YA ms YA pa Search b fes Outlook Express l D Remote Assistance a ae Help ene SHAO Windows Media Player a Run i Windows Messenger ui ki Windows Movie Maker E D Log OFF user ir Thermo Foundation 1 0 E Thermo calibur Turn OFF Computer E oe Audit Viewer Authorization Manager la AutoLogOFf E CRC Validation E Database Configuration ek Instrument Configuration Eksigent Software Drivers 27 of 42 The Xcalibur Software Installation 2 From the Available Devices group click the Eksigent LC Channel 1 and then click Add gt gt to add it to the Configured Devices group list Thermo Foundation Instrument Configuration Device Types ee E Available Devices Configured Devices JW eksigent Eksigent LC 1 Eksigerit ManoLl A5 174 Figure 3 5 Instrument Configuration 3 If you are configuring a NanoLC 1D or NanoLC 2D system from the Available Devices group click the Eksigent LC Channel 2 device and select Add gt gt to add it to the Configured Devices group list 4 Ifyou are using the Eksigent AS1 autosampler from the Available Devices group click on the Eksigent AS1 Autosampler and select Add gt gt to add it to the Configured Devices group list 5 Click the Eksigent LC Channel 1 configured de
28. stered 4 Click OK software by using this utility checking the relevant boxes and clicking Gi Tip If necessary you can later remove the driver from the Hystar Apply ED Eksigent Driver Configuration Utility software Version 3 11 0 Build 120104 Location C Program Files Eksigent Hanol t acalibur Mot Registered Analyst Mot Registered Hy Star CoProgram Files Bruker DattonikiHy Star Eksigent System Driver Mot Registered Calibration Disk Apply Figure 2 3 Eksigent Driver Configuration Utility Eksigent Software Drivers Installation Guide 16 of 42 The HyStar Software Installation Configure the LC system Refer to the Eksigent Software User Guide for setting up and calibrating your mass spectrometer The mass spectrometer must be configured properly prior to attempting to establish control within the HyStar software Mass Spectrometer Configuration using the HyStar Software 1 Start HyStar O x Module Tools View Compass Help lt lt lt lt lt lt Beta Version 3 2 15 0 gt gt gt gt gt gt hystar Hardware Setup Method Sample Table Acquisition Processing Exit Figure 2 4 Main dialog 2 Click Hardware Setup and select an existing configuration file or type in a name for a new configuration My Recent Documents express 00woven hss E nanalc hss Desktop My Documents gr hy Computer SISSAREV lt bly Mebwark
29. t 451 Figure 1 14 The Analyst software acquisition method editor Eksigent AS1 autosampler methods can be created and edited using the Autosampler Method button in the AS1 utility dialog See the Eksigent Software User Manual for more information on creating methods Peak parking Peak Parking is a method that extends the analysis time of MS MS interrogation for peaks of interest eluting from the LC column The LC column flow rate is immediately reduced to a predetermined rate when the initial portion of the peak fraction enters the MS The Analyst software can control the built in peak parking feature of the Eksigent LC devices This feature is controlled within the Analyst software MS method by the Information Dependent Acquisition IDA criteria Eksigent Software Drivers 13 of 42 Installation Guide The Analyst Software Installation 1 Right Click the Period icon to add IDA criteria MS Advanced M5 Acquisition method Acquisition Method Experiment fi Eg Mass Spec 0 000 min i ij Sean kupe Enhanced Product lon EP a Add experiment ly fe Positive Negative T Center Width IY Parameter Range 30 000 amu Product OF Figure 1 15 The Analyst Software MS Acquisition Method Editor 2 Select the Peak Parking tab within the IDA criteria dialog and then select the Enable box The LC flow rate and other parameters can be defined in this dialog IDA Second Level Criteria Peak
30. t 452 2 Eksigent Gradient 1 fe Method Merger For Help press F1 User Mame IN O03 operat 2 Figure 1 4 The Analyst software navigation bar with software devices 1 Start the Analyst software 2 Double click the Hardware Configuration icon in the Navigation Bar Eksigent Software Drivers Installation Guide 8 of 42 February 2012 The Analyst Software Installation Hardware Configuration Editor Hardware Profiles MassSpecUnly New Profile Edit Profile Delete Profile Activate Profile Available Devices Help Figure 1 5 The Analyst Software Hardware Configuration Editor Choose the current hardware profile and either deactivate it or create a new one Click Edit Profile Click Add Device Select the device type Software Application and then click OK o gt oS 2 Available Devices Device Type Software Application Mass Spectrometer 4 0 Converter Integrated System Software Application canes Hep Figure 1 6 The Analyst Software Available Devices dialog 7 The software device will now be listed as part of the profile Select the device and then click Setup Device 8 Select the device type from the list gt Tip Use Eksigent Device CH1 for the LC system or AS1 Autosampler for the autosampler A Installation Guide Eksigent Software Drivers 9 of 42 The Analyst Software Installation 9 Give ita name and click OK Software Application Settings Software applicati
31. t ready LC System Lamp Temperature Stabilizing Off Qz Total Flowrate 5 000 pL min Options Compass Window Help Direct control Diagnostics A B channel Status ready About Vial Inj 1 4 1 1 of 1 15 x 18 x Runtime 00 00 00 01 00 00 1 A Time 0 0 3 0 amp 497 B 503 Inj Vol 5 0 ul IR IR ANRI HNI IRIT an an T Cok Line Vial Position Sample ID Method Store data in _ Sample Table L Sample GQ Reload TAA unkn test 3min m ZF Edit amka 14101148 a m i i gt oe Stop Abort Pause Cont Q bf A Zoom Cursor Stretch PP DA For Help press F1 Figure 2 11 Eksigent LC Device Control Dialogs Installation Guide J i l l k 2 0 25 Time min Chromatogram Additional Signals Wavelength 280 0 From detector JEksigent LC System hystar Sergei Eksigent Software Drivers 23 of 42 The HyStar Software Installation Data acquisition No special steps are needed to configure Eksigent data acquisition in the HyStar software Every time the driver runs all available data is sent to the HyStar software for real time display and storage Stored data files can be further processed in the Post Processing area of the HyStar software t J B Fie Mo P Acquisition View Options Compass Window Help run Running Qa Qb Pa Pb Po A 8 tus n Total Flowrate 5
32. tar Method Editor dialog See the Eksigent Software User Manual for more information about editing method parameters for each Eksigent device Eksigent System Method Parameters i xX Summary Run Conditions Flower Profile Flow Table Detector Method Identification Method ID defaut Column Information Manutacturer Eksigent Technologies particle size 3 oT Type ChromxP diameter 150 am Serial Number length 140 cm Sample Injection Flow Profile Mone Wana Duration 3 min Detection Data willbe acquired at 5 7 Hz This acquisition rate results from averaging 22 CCD exposures of 7 me each The acquisition will continue for 3 minutes collecting 1047 spectra The current spectrum is defined between 200 nm and 380 nm at 1 nm increments The estimated full spectrum data file size is 754 kb Figure 2 10 Eksigent LC Device Method Editor device Note For multi channel instruments each channel is considered an independent Eksigent Software Drivers Installation Guide 22 of 42 Additional instrument dialogs The HyStar Software Installation Access to some of the primary Eksigent instrument control software functionality is provided through the drop down list located in the status box from the main Acquisition dialog in the HyStar software Diagnostics and Direct Control are also provided on this main dialog BeHyStar Acquisition test4 xml Eile Module Acquisition View no
33. ther correctly for the mass spectrometer to trigger the LC device peak parking mode Note If Peak Parking criteria are set too low in the MS method the analysis may never end and will reguire manual termination P Installation Guide Eksigent Software Drivers 35 of 42 The Xcalibur Software Installation The Eksigent LC peak park parameters are configured within the Peak Park Toolbox These options determine what action the Eksigent device performs when activated by external trigger See the Eksigent Software User Guide for more information on these settings PeakPark ToolBox Eksigent SmartPark External Trigger ready Flowrate 4 oo nLimin Flowrate Reference f Mobile Phases fe Column Timing z Ignore Successive triggers torfo Fixed peak park duration jg Figure 3 16 Eksigent Peak park Toolbox Eksigent Software Drivers Installation Guide 36 of 42 Data acquisition The Xcalibur Software Installation Eksigent device data can be passed to the Xcalibur software for real time display and storage in raw data files for further processing in Quan and Qual Browsers ioi x File Actions View GoTo Help wael gt ua auae a 2 Status Acquisition Queue Raw File C calibur D ata data01_060125124311 F 0 0 0 5 1 0 1 5 2 0 2 5 3 0 Inst Method C Xcalibur methods expr1 00 Time min Eksigent LC Channel 1 Running 200 220 240 2
34. tware drivers on systems running Windows XP An Analyst software hot fix is forthcoming please contact your AB SCIEX representative if you have further questions concerning this issue Users with Windows 2000 or NT do not need this fix Systems with Windows XP SP1 Users with Windows XP SP1 must not run in Integrated Security Mode It can be changed by 1 Start the Analyst software Open the Security Configuration tab Select More Click the Security tab Click Single user Click Apply Click OK 8 Restart Analyst software oe S After configuring the hardware profile if the instrument control is still not functioning or if you require the use of other security modes upgrade to Windows XP SP2 and follow the directions for SP2 The upgrade is free and available at http windowsupdate microsoft com Systems with Windows XP SP2 1 Click Start gt Control Panel gt Administrative Tools gt Services 2 Inthe Services dialog locate AnalystService and then click Stop W Tip Do not close the Services dialog LF 3 Click Start gt All Programs gt Accessories gt Windows Explorer to browse to the Analyst software bin directory This is most likely installed on C Program Files Analyst bin Locate and delete msvcrt dll 4 Return to Services select AnalystService and then click Play Return to install the Eksigent software drivers Installation Guide Eksigent Software Drivers 41 of 42 Known Issues T
35. uide 20 of 42 The HyStar Software Installation Device method editors Methods for each device are created within the HyStar software method editor through the Advanced Parameters button LC Method Part Editor test 3min m l X General LU Parameters LC Timetable Signals 1 8 tart data acquisition runtime LE Pump Autosampler UV DAL Detector ela gt Lr pump runtime total run lt Purnp 1 Eksigent LC System Advanced Parameters amp 1 Total Runtime 30 min ee E es vee Ean T p Pump Flow Rate ml min ADDO Min Max efoo a Pressure limits bar jo 9999 Start delay 0 00 mir Runtime 3 0 min Open Save As Print Figure 2 9 Starting the Eksigent LC Device Method Editor Tip All LC method parameters must be set using the Additional Parameters field i and not by using the HyStar Method Editor fields 1 To create or access a set of device methods select Methods from the main HyStar dialog or Edit Method from within the sample table or the Acquisition dialog _ Tip Care must be taken to synchronize the runtime specified in the W Eksigent Method Editor and the general Total Runtime and Acquisition Runtime on the main Method Editor dialog The same applies to the UV wavelength and PDA range See Figure 2 9 Installation Guide Eksigent Software Drivers 21 of 42 The HyStar Software Installation 2 Click Advanced Parameters button on the main HyS
36. vice to select it 6 Click the Configure button The Device Configuration dialog appears 7 Select the correct device from the drop down list from the Device Configuration dialog Eksigent Software Drivers Installation Guide 28 of 42 The Xcalibur Software Installation 8 Set other desired Device Configuration parameters Eksigent Nano LC xX System Device ja Advanced System Configuration Eksigent Device NanoL c 20 COM port cont Injection Yalye None System shut down if idle more than 120 min Figure 3 6 Eksigent Device Configuration 9 Click OK 10 If it is configured click Eksigent AS1 Autosampler 11 Click Configure to open the Eksigent AS1 Autosampler Configuration dialog SparkUe Config Dialog Autosampler COM Port 1 16 2 Autosampler ID 21 only 21 i Cancel Figure 3 7 Eksigent NanoLC AS1 Autosampler Configuration 12 Set the COM port and then click OK to save the settings 13 After configuring the Eksigent devices click Done in the Xcalibur Instrument Configuration dialog to save the configured device values Installation Guide Eksigent Software Drivers 29 of 42 The Xcalibur Software Installation Thermo Foundation Instrument Configuration Device Types All Available Devices eksigent Eksigent LC 1 Figure 3 8 Eksigent NanoLl 45 174 Configured Devices eksigent Eksigent LC 1 Eksigent NanoLC 451 ZA Instrument
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