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ClarityChrom Instrument Control 3.0.7 Software Manual

1. ccccccccseeeeeeeeeeeeeeseeeeeeneeeeeenaees 100 Device Monitor Virtual Detector cc cccccceececseseeceeeeeceeeeseeeeesaeeesseeeesaaeeees 103 Device Monitor Assistant ASM ZIL 103 Device Monitor Autosampler 103 Device Monitor Column OVENS c ccseeeccceceeseeceeeeeeeeceeecaeeeeeeeseaeeeeeeseaaeees 104 Device Monitor Knauer Switching Valves ssssssesssnnnneseessrrnrnrrressrrnnereeee 104 Device Monitor Fraction Collectors cccccccssseecceeceesseceeeceesseeeeesaeeeeeeeeaeeees 105 Brief Description 5 Brief Description The chromatographic station ClarityChrom is an effective tool for the acquisition processing and evaluation of data from any gas or liquid chromatograph with analog output It is possible to acquire data using control modules also from chromatographs equipped with digital output In the maximum configuration it is possible to measure on up to four chromatographs simultaneously of which each may be equipped with up to 4 detectors The station is equipped with support for automatic co operation with chromatographs and autosamplers ClarityChrom offers the possibility to work under fulfillment of the requirements of directive 21 CFR Part 11 of the FDA The ClarityChrom station automatically processes all data acquired using CSW stations ClarityChrom can be delivered either as an analytical or a preparative version The preparative one includes all features of the analy
2. Serial Number Solvent Recycling Hack Type 12 13 mm tubes Number of Viale 144 Vial Yolume rl 10 00 T Use RFID Seral Fort EOM E Port Settings Cancel Add Into Help Configuration window for the Foxy R1 FC Foxy R2 FC Name Enter a descriptive name for the oven Use a name which is unique within the instrument Serial Number Type in the serial number of the collector you are using Rack Type Select the desired rack type from the drop down list Please make sure that in the configuration the same rack type is selected as in the collector s setup Note that it is allowed select a rack type with less number of vials than in the collector s setup If the number of vials of the selected rack type is higher than for the rack type selected in the collector a message Cannot establish communication will be shown Please make sure that in the configuration the same Rack Type is selected as in the collector s setup Number of Vials The Vials number will be displayed automatically according to the rack type Use RFID The fraction collector R2 allows identifying the rack type using RFID technology If this option is enabled the driver will read out the rack type from the collector Even if the configuration screen of the R1 also allows enabling this option the Foxy R1 does not support this feature Vial Volume The rack depended value will be displayed automatically However you a
3. PDA Method Displays the PDA Method for several settings when the PDA Module is active Detailed description is provided in a separate PDA manual Thermostat Column Ovens Fig 122 The method setup thermostat window includes the column oven setup tab and the tab independent button Th Status Th Status displays a status summary Select thermostat and the field Enabled are not accessible and only for information as soon as a thermostat is configured and added to the active instrument Method Setup Final Test S1LPG_S2600_Midas 1 Select Thermostat IV Column Oven Method Column Oven Setup Temperature Time Temp DEES 30 Wat Time min 0 1 Th Status Description Status 242 C Event Table AS LC Gradient Measurement Acquisition PDA Thermostat Integration PDA Method Ce OR Cancel Apply Report Help Thermostat Temperature For details on the spreadsheet operations refer to the section Creating a Time gradient Program on page 74 Setup and Programming Fraction Collectors 91 The oven program profile will be adapted automatically to the spreadsheet inputs Wait before injection The settings Wait with tolerance C F K dependent from the settings in the Instrument Method Sending menu and Wait time min allow the autosampler to wait for an injection until the start temperature is reached and the wait time is out The system will wait for that time afte
4. SeU D EE 65 RF 10Axl detector ceecceceeseeeeeeee eens 35 RI Detector PACOUISIION EE 78 Configuration cccceeeesseeeeeeeeeeeeeees 27 SGD we cedacitemenenedcaneden tins 78 81 S 100 CONfiQuratiOn sorsi 22 23 Device MONO E 97 Ee ee 73 S 1000 Configuration cccceeeeeseeeeeeeeeeeeeeaes 22 Device monttor ecceceeseeceeeeeeeees 97 98 SS TU EE 73 S 2300 Configuration ccccceeeeseeeeeeeeeeeeeeees 27 Device monttor cceseeeeeceeeeeees 98 102 SOTO E 77 78 S 2500 COMM QU ATON EE 29 Device monttor cceeceeeeeceeeeeeeeee 98 99 SOTO E 79 S 2550 Device MOnNItOTF EE 99 ESS WEE 31 S 2600 Configuration cc cece eeeeeeeeeeeeeeeeeaeeeeees 31 Device montor 99 EE ebe 79 S 2800 Configuration cccccceeeeeeeeeeeeees 33 38 DEVICE MONT oisinnean 99 SEUD GE 79 Sampling Hate 78 79 82 5G EE 88 101 Sequence die ele EE 14 S Cha INUIMDOT aranna 46 Setup Fraction Colector EE 90 KSE 73 Switching Vahves 85 87 Systems and Instruments 00 64 SUMS EEN 33 35 101 Signal EXC DEE 77 Jee EEN ER RRE 92 MOdE EE 88 101 WIG tel date EE 15 See 93 Smartline Oven AO 50 SOlVENt FECYCIING cceeeeeececeeeeeeeseeeeeeeees 54 Spark Auto sampler aannnnnnnono0annnnnnnnae 49 Spectrofluorometric Detector 02 35 Seed EE 102 01 gt 1 41 0 EE 102 Gpreadeheet innan inai ai 73 elle 75 Supported instruments
5. Select Detector Virtual Detector X Iw Enabled Detector Method Detector settings Argument settings Advanced settings IV Argument gt Argument Y Source Time e Source Resulting Formula wi Kr o Det Status Description Status Event Table Measurement Acquisition PDA Thermostat Integration PDA Method Calculation Advanced Cancel Apply Report Help Acquisition virtual detector argument settings Setup Systems and Instruments 85 Fig 111 Fig 112 Argument X X and Y are optional arguments use of these can be switched off A result formula can be calculated without either X nor Y with only X or with both X and Y single Y is forbidden If using X or Y is switched on X and Y signs must be used in the result formula expression After checking this field the pull down menu of the Source becomes accessible The options are Time Data File and External Source Time With this selection no further settings for Argument X are possible The argument value is equal to the current time value Data File The value of this argument is calculated using the corresponding point in the selected data file For example for time 10 seconds if sampling rate is 1 ten points will be extracted from the data file The Settings button will become accessible and clicking on it will bring up the following dialog box Select Data File EA Clari WORK DATASExample pr Shift Ti
6. window will be displayed containing the symbols of the connected chromatographs After clicking on the chromatograph image and entering a username the Instrument window will be displayed This window is used for acquiring and processing data from the chromatograph to which you are connected The ClarityChrom station works with what we call instruments All detec tors that are connected to the same instrument will share a common time base Main ClarityChrom Window The main ClarityChrom window is designed to set the station s configuration select access rights and basic directories for saving data _ Open Eua SCH Teimer Instrument SERED RE SERA PE em aen SS ee name symbol System Login Logout Lock wiew Help User Account Instrument 1 Instrument 2 System Reg Configuration a T ge e Gesi Closed Administrator i Instrument Username R symbol For Help press Fl Fig 13 Main ClarityChrom window 5 Je The System Configuration dialog opened using the a geen H icon or System Configuration command is where Je number of connected chromatographs their names ae the selection of displayed symbols and the type of iert ie directly controlled equipment are all established As S 2800 PDA Instrument 1 The User Accounts dialog opened using the i icon or System User Accounts command is where aname and password for each user is allocated and the extent of authority for individual operation types access
7. COS TG ASIN ATG EXP LG log10 LN In SH hyperbolic sine CH hyperbolic cosine TH hyperbolic tangent SQRT ABS RAND randomizer Constants PI O 3 141592653 E e 2 71828183 Operations Addition Subtraction Multiplication Division Power unary plus and minus Method Setup C Clarity WORK2WDefault 2 Select Detector Virtual Detector e Detector Method Detector settings Argument settings Advanced settings Iw Use Advanced Settings r e 1 om x 2110 00 x Det Status Description Status Event Table Measurement Acquisition PDA Thermostat Integration PDA Method Calculation Advanced Cancel Apply Report Help Acquisition virtual detector advanced settings Selecting this tab and activating the option Use Advanced Settings a time table for changing the formula during a run becomes accessible Assistant ASM 2 1L Setup Due to the modular system there are a lot of possible configurations for the ASM 2 1L There is not ASM setup window which includes the setup of the ASM 2 1L modules All modules will be shown as single devices pumps detectors valves MultiValve FC In the appropriate Select bar the ASM 2 1L name and the module name will be shown Setup Systems and Instruments 87 Fig 115 Fig 116 Fig 117 Method Setup Default1 Select Valve IV ASM2 1L Valve Met
8. Hem Add Into Ed Own port recycling valve setup Number of Valves Enter the number of valves which build up the multi valve fraction collector The number is in practical patterns unlimited Vial Volume ml The rack depended value will be displayed automatically However you are allowed to overwrite it that the volume matches with the volume of your vials The software uses this volume to calculate the filling level in the vials If any fraction is larger than the set volume the next free collection position will be used for continued collection to prevent that a vial will be overfilled Please refer to the chapter Setup and Programming Fraction Collectors for more details Please make sure that in the Vial Volume corresponds with the real volume of the vials you use 60 Fig 77 Configuration Systems and Instruments Number of Vials The vial number will be displayed automatically Valve Each involved valve has to be configured separately The number of valve tabs is given by the set number of valves Serial Port Select for each valve to be configured from the drop down list the serial port number for the communication port on your PC where the valve is connected Valve type The drop down list offers 2 6 12 and 16 position valves Select for each valve the desired type Configuration Foxy R1 R2 Fraction Collectors FC Foxy R1 Configuration FC Name Fo R1 FC Tubing Parameters
9. and each unit corresponds to 5 microvolts of signal enter 1 5 0 2 44 Fig 57 Configuration Systems and Instruments Y Axis Offset Type in the correction offset which will be used for the signal recalculation The Y Axis offset must be given in the same units as the Y axis is labeled Consider the formula DU displayed units DU MS Y M Y O MS measured signal in micro volts Y M Y Axis Multiplier Y O Y Axis Offset As an example If the signal offset is 2 mV and the multiplier is set on 0 001 as the Offset value 2000 must be entered Analog Output Check this option to enable the analog output control feature for this channel This can be used to control devices which are currently not supported by ClarityChrom Mode In the case that the device connected to the analog output of the Knauer Interface Device is a detector or an LC Pump additional software support can be used The following analog output control modes are currently available Analog Output Mode Description Generic Device Default mode when using the analog output control feature The device is controlled by setting the analog output voltage directly based on voltage values entered by user LC Pump This mode represents the device as a fully functional pump Both LC Gradient and Auxiliary pump support is available from the ClarityChrom for this device The device is controlled by setting the analog output voltage automatically recalculated fro
10. than one transport vial in a Sequence you must create additional methods with the desired transport vial Wash The needle wash options are never between injections and between vials Wash Times Select how often the needle should be flushed 1 9 times is possible Tray Cooling C The temperature only can be set if the option field is checked Preparative Mode If the prep extension is installed enable the Preparative Mode check box If this option is enabled the injection loop volume and the syringe volume available in the System Settings tab have been set to fixed values loop 10 ml syringe 2 5 ml In Prep mode only the Injection Mode Partial Loopfill is available Inputs amp Outputs The Inputs and Outputs available via the separate connector I O in the autosampler s rear panel are not required to control the autosampler by software Method Setup Method 1 Select Sampler E 45 3950 Sampler Method Mode Time and Temp Inputs and Outputs ix Methods System Settings Tray Use Relay as Inject Marker v Event Program Uselnput1as Nest Injection D Timecminy Comments Next Injecti Use Input 2 as ext Injection e aes End Time min Status Tray Temperature 29 C Ready 45 Status Measurement Acquisition Integration PDA Method Calculation Advanced OK Cancel Send method Report Audit Trail Help Autosampler Inouts amp Outouts Use Relay AS Select Inje
11. than the set limits Fraction position This field is for information purposes according to the settings in the parameter window Either the defined vial position is displayed or Next for the first free vial position Additional possibilities are Waste and Solvent Recycling Please note that the touch pad of the Foxy R1 R2 FC will not been blocked if the device will be controlled by software Therefore it is highly recommended not to use the touch pad under software control Otherwise the vial position of fractions and others may not been stored correctly Editing of Fraction Collection Program Fig 129 Edit As an alternative for the direct programming of the fraction table or as an additional option click the Edit button to open the FC program editing tool which allows also for graphical programming of fractionation FC Parameters at 0 00 min Loaded data M Temp Clarity FRC Data Clarity Data 33663 118 2 P4a 23dat U Detektor PRM Load Cancel Fraction Collection Program 10 RE Se Peak Beg Level Units 685 688 Single Event Unconditional Waste tai wi Start if signal level is lower than 3 48 Peak Recognition Signal Level next 5 84 Peak Recognition next Peak Beg Slope Units min 263 694 EE Action Peak End Level Units 685 688 f AND C OR Peak End Slope Units min 269 694 Please notice that the parameters will be used in the Units selected for th
12. whatever comes first User code Fig 5 Installation license selection Enter the user code of your license dongle If you install a DEMO version skip this step The user code can also be entered after the software is installed in Help User Code During installation the installer will ask for the installation of some drivers Chromatography Devices Installer Welcome to the Chromatography EA Devices Installer This wizard will walk you through updating the drivers for your Chromatograpy Data Station ClarityChrom To continue click Next Fig 6 Installation driver installation window Installation Fig 7 Fig 8 Fig 9 Click on lt Next gt to install the drivers Chromatography Devices Installer Congratulations You have finished installing drivers for your Chromatograpy Data Station Devices The drivers were successfully installed on this computer fou can now connect your device to this computer If your device came with instructions please read them first Driver Name Status we s y i v Datadpex Ltd cswcb20 Ready to use Clarity Chrom v Datadpex Ltd cswint7 Ready to use E V Datadpex Ltd cswint9 Ready to use a Installation driver installation finished If the installation is finished the system must be rebooting 4 ClarityChromPrep v 3 0 7 662 Setup Completing the ClarityChrom Uu KNAUER Preparative Setup Wizard Y
13. window The OK button is active only after defining the serial port number The virtual fraction collector is a tool to optimize the fractionation without need for sample or eluents Therefore only opening and closing the configuration window would be sufficient However you have two areas for individual settings Configuration Completing Instruments If all devices are configured you must add them to an instrument using the a button Add selected sub devices If you want to control more than one system instrument you must add the devices to the appropriate instrument Select the desired instrument with a mouse click on the instrument tab If a PDA detector is Configuration Systems and Instruments 63 included you must first select an instrument type which includes PDA LC PDA or GPC PDA please refer to the chapter Configuration Instrument Type A device can only be added to one instrument If a device has been added to an instrument the instrument name will be shown on the left handed side of the configuration window in the column Used Configuration Instrument Type Select the correct instrument type It depends on the license options on your USB hardware key dongle To control a GC system select GC for an HP LC system LC If a PDA detector or a detector with scan functionality is in the system select LC PDA or GPC PDA if additionally the GPC license option should be active To use the GPC f
14. 1 32 min 0 95 ml will be displayed in the case of single fraction collected to the vial If more than one fraction is collected into the vial then the information contains the sum volume collected into the vial Vial 1 1 24 ml multiple fractions A mouse double click on a vial during a run will force collection of the eluate into that vial Direct Control operation Mouse double click on the vial where the fraction is being collected during a run will force the eluate to be directed to waste Mouse double click with pressed control lt Ctrl gt key on a vial with a collected fraction when it is not running will force the corresponding chromatogram to be opened INDEX INDEX SD DR CC EE 33 35 50 Hz 100 Hz Oppon eee eee 41 ee RUE e EE 77 Channo EE 82 Channel depending Settings 80 Ale Sege EE 71 LEE See AS3950 Analog Output ccccseeeeeeeees 42 80 81 Analysis MONIONNO BE 16 SUERG e Ee e 16 BS SC OO MEN 49 66 PRS 3900 e vite eed eterna elated 49 66 BS S950 WEE 49 Auto sampler eisrean a a aa 66 EE a 49 System Settings cceeeeeeeeeeeeeeeees 71 EE 72 PUUTOSIOD E 76 PAULO e SEN 44 Auxiliary Humpe 26 30 AXIS Multiplier cccceeeeceeseeeeeeeeeeeeeseees 42 ANS OMS EE 42 Briet riet NEE 81 88 101 Bue CHG GOO esicee sick coh a ois cectaceeetes 60 Calcula EE 90 AID FAT OW EE 17 WIN OO EE 13 Channel Configuration cccccccssseeeeees 41 Channel depending s
15. 2550 is connected via LAN and only one channel is enabled in the configuration S 2550 also 20 50 or 80 Hz can be selected for this channel S 2550 channel 1 For the detector UVD 2 1S the maximum data rate via LAN connection is 50 Hz The relation between the selected sampling rate and its suitability for minimum peak width is displayed below in the setup window It is recommended that you use the peak width s see Graphical Events Programming or if included peak report to determine the optimal sampling rate for your chromatography A peak should include in minimum 20 data points better 30 40 Autozero at start Check this field if you wish the signal to be zeroed at the moment a measurement is started to define the current signal as the baseline Autozero at WL change Check this field if you wish the signal to be zeroed at the moment of any wavelength change during a run to define the current signal as the baseline The option is inaccessible fixed with the UV detector 2500 Lamp OFF at the end of run Check the lamp OFF box to switch off the detector lamp after a measurement is finished This option may be used for the shutdown methods Detectors UVD 2 1L S 2520 and 2550 If the lamp was switched off by software Device Monitor or method and you switch off the detector then on the next detector start the lamp is still off it must be switched on manually in the detector s own setup or with the software via Device
16. Acqure 3D Date vtwis Unt mA FlowCet S 2800 configuration window Name Enter a descriptive name for the detector This name will appear on the detector tab when the instrument setup or status windows are displayed The default detector name will be suggested automatically by selecting the detector type Use a name which is unique within the instrument Serial Number Type in the serial number of the detector you are using Channels Select the number of the channels 1 4 to be used for data acquisition 36 Fig 49 Configuration Systems and Instruments Chn Name 1 4 Enter a descriptive name of the data acquisition channel Inversion of Signal Chn 1 4 Check the Inversion of Signal box to invert the data for the acquisition channel Interface For the diode array detector the choice is PCI or Ethernet The PCI RS 485 interface card is only available for the detectors K 2700 K 2800 The access to the IP Address field is blocked if PCI was selected The IP address of the PDA detectors can be set either to a fixed address or to obtain from a DHCP server The communication setup is available via serial port and appropriate software as Hyperterminal Please refer the detector manual for a detailed description Interface LAN IP Address The IP address can be obtained automatically from the device by clicking on the button to search for the device It will not be shown in the IP address field i
17. DIG IN GROUND digital ground Autozero Signal Connections lf your detector has an autozero option and you want to use the feature to zero the baseline signal at the start of data acquisition you must connect an autozero signal cable to the Interface Box The autozero signal is connected to one of the digital output connectors on the rear panel of the Interface Box as shown in Fig 60 It must be the same channel as it is used for data acquisition Configuration Systems and Instruments 47 AN OUT DIG IN RELAY gt Fig 60 Wiring example Manager 5000 IF2 and K 2501 This example shows the connection lines between the Interface Box or Manager 5000 and the Knauer K 2501 detector Configuration Assistant ASM 2 1L The Azura Assistant ASM2 1L is a modular instrument that allows combining up to 3 devices modules The following devices can be included pumps P2 1S P4 1S detector UVD 2 18 Knauer valve drives with 2 6 12 and 16 positions Valco valve drives with 2 6 8 10 12 and 16 positions Configuration rules Two pumps are only supported as HPG both pumps must have the same pump head three pumps are not supported Pumps cannot run in a HPG if they are installed in different ASM2 1L housings Only one UV detector is allowed One valve drive can be used as a fraction collector if the Knauer Fraction Collector Control option is installed and the appropriate license option is used Cascading fracti
18. F1 Overlay nj Yolume ml Dilution A A nay X K SA Bn SS ah ai EA SC oh ie A A A ba A ay A tA de Af Chromatogram Result table Other table tabs Setting type of result modifications calculation Fig 15 Chromatogram window Calibration Window The Calibration window opened by clicking the icon is used for creating modifying and displaying calibration curves i a The global calibration table and calibration standard chromatogram are located in the Compounds tab Calibration Calibration Switch to other station Calibration Calibration Global file selection Standard display windows level selection type selection calibration table My GC Calibration ETHANOL lt ISTD File git Display GAlibration View Window Help E fil Ak ES ert wy AE Sx e A a SE i Liautomste Aibration Signal 1 Left Right Resp Level 1 Used Compound Name Reten Time Peak Type RB S EE ZE Resp Fact Rec No H ETHANOL 2 308 0 200 0 200 Ordnr A 0 0000 22 5900 0 400 0 0177 H T BUTANOL 2 862 0 200 0 200 EI A 0 0000 50 4277 1 000 0 0198 1 CALIB Cal04 2 322 1 F 1 3 Retention Time Compounds A ETHANOL A T BUTANOL For Help press F1 Chromatogram Cal04 Display of global calibration table Detailed display of alibration standard and calibration standard individual compounds name Fig 16 Calibration window Switch to the tab with the name of the compound here displayed as ethanol to display its calibrat
19. Got LC Gradient Setup Options Min Pressure Enter the minimum pressure allowable for your pump The pump will be stopped when pressure drops for more than one minute the set value The zero setting disables the shut down due to minimum pressure Max Pressure Enter the maximum pressure you want allow for the system The default value always corresponds to the configured pump head A setting above this value is not Setup Systems and Instruments 77 allowed The pump will be stopped immediately when pressure increases the set value Solvent 1 4 The configured solvent components can be named activated and deactivated Only activated components will be displayed in the gradient table and delivered during the run Measurement Fig 102 Method Setup My method MODIFIED Common for all detectors Method Description jw Enable Autostop Column Run Time 0 min Mobile Phase Flow Rate Pressure Detection Iw External Start Stop Temperature C Start Only Note for let Pest Not supported by KNAUER instruments Porat toe Up A s i Cue L Event Table 4S LC D I Measurement Apauiston PDA Thermostat Integration PDA Method Ce 4 gt Cancel Apply Report Help Measurement This window describes in detail measuring conditions that includes determining the duration an analysis should take place When invoked the Method Setup Measurement dialog will be opened
20. Serial Port COMI ei e nm ba boer e SCH Add Info Caneel Recycling valve setup For an optional solvent recycling an additional recycling valve may be necessary Check the option Recycling Valve For this a separate Serial Port is to define An additional Knauer valve is required for the recycling The settings for the volumetric delay will be displayed without access for changing The settings are valid as well for the recycling valve as for the fractionation valve Therefore the capillary between the recycling valve and the fraction collector should be as short as possible The Knauer fraction collector 3050 and the MultiValve fraction collector can also use own ports for recycling This option can be activated alternatively Add Info Click the Add Info button to add the collector specific description or comments Configuration Systems and Instruments 57 Fig 72 Fig 73 Configuration Smartline Fraction Collector 3050 FC 3050 Configuration Hack Tubing Parameters FC Name P 3050 FC Rack Type A 12 mm vials low e seral Number Rack Type B E mm vials low e Solvent Recycling Interface LAN hi a Rack Type C 12 mm vials low e Rack Type D 13 mm viale low gt Add Info IP Port i000 Number of Vials 192 Help W Use S N to identify the instrument Vial Volume ml 1 5 H coed Ir Configuration window for the Smartline FC 3050 Name Enter a descrip
21. acquisition settings are configured correctly Note that in this case pressure units are hard coded to the current pressure units selected by the user MPa bar or psi Factor for the Detector mode Enter the conversion factor used for recalculation from wavelength values into the corresponding analog output voltage Minimum WL Enter the minimum wavelength for the configured detector Maximum WL Enter the maximum wavelength for the configured detector When analog output options configuration is complete click OK to exit the dialog and return to the Knauer Interfaces configuration dialog Add Info Click the button Add Info to enter more detailed description of the channel When complete click OK to exit the dialog and return to the system configuration window The OK button is active only after defining the serial port number 46 Fig 59 Configuration Systems and Instruments Detector Connections Up to four detectors can be connected to the Manager 5000 5050 or IF2 The detector connections are identical Always use a shielded twisted pair cable to connect each detector to the interface This minimizes electrical noise Connect the analog output lines from each detector you want to acquire data from to one of the four analog connections labeled AN IN 1 through 4 It is not possible to use the cinch connector and the WAGO connector from the same channel for different instruments They are not independent of ea
22. all connected devices from the same model type will be listed If the device is connected via RS 232 normally just one device will be displayed via LAN several devices may be shown The window below shows two pumps model S 1050 connected via LAN Select the device you want to add and click on the lt Select gt button to read out the selected device and download the information into the configuration window Device Information IP Address Serial No 172 26 2 11 1050 AAA 300003 172 26 2 15 1050 AAA122900002 Refresh Select Device Information window with 2 pumps model S 1050 24 Configuration Systems and Instruments Configuration Knauer Pumps The following Knauer pumps are supported by ClarityChrom AZURA P 2 1L AZURA P2 1S P4 1S Smartline Pumps S 1050 S 1000 S 100 Preparative Pump 1800 and WellChrom Pumps K 1800 K 1001 K 1000 K 501 K 120 P2 1L Configuration Name P21 L Gradient Mode LPG v Si Serial Number F4D122800003 Interface LAN e Bj Head 250 ml X Maximum Flow 250 00 mL min Maximum Pressure 22 50 MPa IP Port H 0001 Use S N to identify the instrument M Loak Sensor Sans Medium E Do not stop pump when closing Clarity I Auxiliary Pump Add Info instrument Cancel Help Fig 27 Pump P 2 1L configuration window Pump S 1050 Configuration Name 1050 Gradient Mode lsocratic v E Serial Number Interface EM Head 10 ml O
23. button on Knauer Interfaces configuration dialog will show the devices connected to the selected interface Click the button Select to select the desired device The serial number as well as any additional information for the selected device will then be automatically entered into the configuration dialog Smartline Manager 5000 5050 IF2 The loading configuration procedure checks the type of connected hardware and reads the current configuration In the case that the device is currently switched on and is compatible with this control module the configuration will be read from device The user can approve the use of this device with this instance of the control module or reject the result of this search and try another serial port The approved information read from the device is used to update the current configuration In the case that there are no devices connected to the selected serial port or the device is incompatible an error message will be displayed Success J An IF2 with serial number 58 was Found on COMB The detected fintmvare version is 1 05 Loading configuration success message for the Smartline Manager 5000 5050 IF2 Knauer HPLC Box The loading configuration procedure will open a device information window that displays the list of devices connected to the currently selected interface The user can use the Refresh button to update the list of 42 Fig 55 Configuration Systems and Instruments devices and
24. device by clicking on the l button to search for the device It will not be shown in the IP address field if the option Use S N to identify the instrument is enabled If the device receives the IP address from a DHCP server the IP address may change with every start of the device However the identification via S N allows finding the device also if the IP address is different from the address during the configuration The IP address can only be entered manually if the option Use S N to identify the instrument is disabled IP Port The IP Port number used for communication can be entered here Please use only port 10001 for Knauer devices Use S N to identify the instrument If this option is disabled the IP address of the device can be entered manually This can be either a numeric IP address or the network name of the device The AZURA pump P 2 1L only communicate via LAN with ClarityChrom ClarityChromPrep We highly recommend using the S N and not the IP address to identify the instrument if the devices receive their IP address from a DHCP server because devices may automatically receive a new IP address when reset Interface Click the button to get a list of all devices of the selected type connected to the selected interface The Device Information window Fig 33 allows for the configuration information to be read directly from the connected devices Click on a device and on the Select button to copy the configuratio
25. display shows RS 232C _ Enter to Enable Press the key lt enter gt The display shows RS 232C _ Enable for only about 2 seconds then the default screen will be shown Now the detector is in serial control mode and can be controlled by software If the detector is switched Off and On it will start again in RS 232 control mode During the boot procedure the RS 232 control mode will be shown very shortly on the display 40 Fig 52 Configuration Systems and Instruments To leave the serial mode press the lt CE gt and lt del gt key at the same time Now the keypad is accessible but the device cannot be controlled by software Fe The driver for the model RF 20A Axs only supports the firmware version 0 9 This firmware is only available through Knauer The control is compatible with model RF 10AxI and will not support the advanced features of the model RF20A Axs Fe The maximum data rate for the RF 10Axl using the serial port is 0 5 Hz for the RF 20A Axs 5 Hz For higher data rates the 1 V Integrator Output with a Knauer interface must be used Fe Both detectors may not work on additionally installed serial COM ports It is recommended to connect it with a computer s onboard COM port Configuration Conductivity monitor Smartline 2900 The configuration window of the detector S 2900 is nearly the same as for the detector S 2500 Please select the serial port COM port the detector is connected and
26. enter the detector s serial number The serial number cannot be read out from the detector Configuration Virtual Detector Name Aus Units Cancel Help Virtual detector configuration window The configuration of the virtual detector only includes optional settings The virtual detector allows e g to re draw already existing chromatograms to optimize the settings for fractionation or consolidate the signals of two detectors into one chromatogram Name Enter optionally another descriptive name for the detector Use a name which is unique within the instrument This name will appear on the detector tab when the instrument setup or status windows are displayed Y Axis Units Fill in the units of your signal to be displayed on the y axis e g microvolt AU MPa etc depending on the detector type An interface device acquires your analog signal and stores it in microvolt If you want to store and to display the signal in different units you must use the correct multiplier Configuration Knauer Interfaces The Manager 5000 5050 IF2 and the Knauer HPLC Box can be configured to either acquire data or control devices not supported directly in ClarityChrom via Analog Out The interfaces can be found in the section LC as all pumps A Knauer Manager 5050 must be configured as a Manager 5000 Note that the analog and digital outputs of the Knauer HPLC Box are only supported for the hardware revision 3 Knauer seri
27. enter the initial flow and composition in ml and respectively Then for time in minutes you can define the values for any desired time The minimum time difference between two lines is 0 02 minutes Setup Systems and Instruments 75 Fig 100 All time programs are displayed in a spreadsheet similar to the one shown below Although the information in the fields will vary the spreadsheets always support certain basic features Method Setup My method MODIFIED Common for all detectors Gradient T able w mL min Fill Down Ctrl F Fill Series v Editing tools for the time program Each row is assigned a program line number and time in minutes followed by columns for information for each time in the program Rows and field information can be cut copied pasted and cleared To open the menu with these commands right click anywhere within the spreadsheet To select a field click on that field to highlight To select a row click on a number in the column to highlight the entire row To select the entire spreadsheet use the right click menu and click the Select All command Certain spreadsheet fields will have choices available for you to select Fields with selections available will display a combo box button when the field or row is selected with the cursor Click the button to display the available choices Cut This command will cut the current selection and place it into the clipboard You can
28. external signal only starts analysis Start Restart The external signal terminates the analysis in progress and at the same time starts a new one The first signal starts the analysis the second signal terminates it and at the same time starts a new one This is known as the continuous measurement This option is not support from Knauer devices Start Stop The external signal starts and stops an analysis The first signal start the analysis the second signal stops it etc This option is not support from Knauer devices Up The station reacts to a voltage change in the relevant controlling input from low lt 0 7 V to high gt 3V or in other words to a closed contact Down The station reacts to a voltage change in the relevant controlling input from high gt 3V to low lt 0 7 V or in other words to an opened contact KNAUER devices do not support the features Start Restart Start Stop And Down Solely use Start Only and Up options Acquisition Detector Method Fig 103 ClarityChrom does not allow running a method if no detector is configured The Method Setup Acquisition window includes the detector selection one or more different tabs as described below and a tab independent buttons Det Status Select Detector and the Enabled check box remain without access if only one detector is included and this detector works with only one channel If more than one detector or a detector with more than one
29. for signal measurement voltage range sampling rate etc FAS Integration Integration table e Calculation Parameters for setting the type of calibration calculations AS Control Direct control of the autosampler LC GC Ee Control Direct control of a chromatograph First Analysis This chapter illustrates an exemplary model analysis that is designed to help demonstrate basic practical work procedures with the ClarityChrom station Naturally however this model does not address all of the stations capabilities Program Start One of the ClarityChrom station s key advantages is its ability to measure immediately without having to make any lengthy preparation or settings Clicking the ri icon on the desktop or by selecting Start Programs ClarityChrom ClarityChrom Chromatography Station starts the program Click on the image of the gas or liquid chromatograph to open the Instrument window You can immediately run an analysis measurement by clicking the 2 icon and using the Run button in the Instrument window However in this procedure we will first explain certain functions before moving on to the measurement Signal Monitoring y Sg Click the icon to display the current signal from detectors and Ly check for drift and noise Deszek Clicking the icon or by using the File Exit command close the s window Measurement and Evaluation Parameters Certain parameters voltage range sampling rate etc m
30. maU sl FlowCet S 2600 configuration window Name Enter a descriptive name for the detector This name will appear on the detector tab when the instrument setup or status windows are displayed The default detector name will be suggested automatically by selecting the detector type Use a name which is unique within the instrument Serial Number If the configuration information from the device have been transferred using the button the serial number field has been filled automatically Otherwise type in the serial number of the pump you are using Channels Select the number of the channels 1 4 to be used for data acquisition If you wants to acquire data from the detector S 2550 with higher than 10 Hz max 80 Hz only select 1 channel Chn Name 1 4 Enter a descriptive name of the data acquisition channel 34 Configuration Systems and Instruments Inversion of Signal Chn 1 4 Check the Inversion of Signal box to invert the data for the acquisition channel Y Axis Units Select the type of units to be displayed on the y axis of your chromatogram from the drop down list The choices are AU Absorption Units mAU 0 001 AU or WAU 0 000001 AU Interface RS 232 S 2550 S 2600 only COM Port Select from the drop down list the serial port number for the communication port on your PC where the instrument is connected For the S 2550 the maximum data rate for one channel using serial commun
31. next free collection position will be used for continued collection to prevent that a vial will be overfilled This can only be calculated if the flow rate is fixed over the run time Please refer to the chapter Setup and Programming Fraction Collectors for more details Make sure that the value for the Vial Volume matches with the volume of your fractionation vials Serial Port Select from the drop down list the serial port number for the communication port on your PC where the instrument is connected Serial Port Click the button the get the configuration information of the instrument connected to the selected interface Select your device by a mouse click and click then on the Select button Device ID only Buechi C 660 Select from the drop down list the Device ID of the instrument connected to the serial port Read the User Manual for the corresponding fraction collector to get more information about the Device ID Use RFID If your fraction collector supports this function check the box to allow for automatic recognition of the rack by RFID chip Port settings For some devices you can set the configuration of the serial communication port Be sure that it matches with the port settings on the device Read the User Manual for the corresponding fraction collector to get more information about the required settings When complete click OK to exit the dialog and return to the system configuration
32. of the device can be entered manually This can be either a numeric IP address or the network name of the device The communication port of the fraction collector S 3050 must be select using a switch on the collector s rear panel We highly recommend using the S N and not the IP address to identify the instrument because devices may automatically receive a new IP address when reset 58 Fig 74 Configuration Systems and Instruments Interface Click the button the get a list of the instruments connected to the selected interface The Device Information window allows for the configuration information to be read directly from the connected instruments Device Information Sena Ne 192 168 171 237 53050 Device Information dialog The Device Information dialog displays a list of the devices of the given category pumps detectors etc found on the selected interface The IP Address for LAN device only the Device Type the Serial Number and the Software Firmware Version will be shown for each found device Refresh Click the Refresh button to actualize the list of found devices Select Click the button Select to select one of the devices The serial number as well as any additional information for the selected device will then be automatically entered into the configuration dialog When complete click Close to exit the dialog and return to the system configuration window Rack Type Select the
33. on the y axis of your chromatogram from the drop down list For the RI detectors the only choice is RIU Detector Type WellChrom series only Select the type of the detector you want to configure from the drop down list The choices are K 2300 K 2301 K 2400 and K 2401 Add Info Click the button Add Info to enter more detailed description of the detector When complete click OK to exit the dialog and return to the system configuration window The OK button is active only after defining the serial port number Configuration Knauer UV Detectors The supported Knauer UV Detectors are the AZURA UVD 2 1L and UVD 2 18 detectors K 2000 K 2001 K2500 K 2501 of the WellChrom series and S 200 S 2500 and S 2520 of the Smartline series The detectors with PDA or scanning functionality K 2600 S 2550 and S 2600 are described separately Please note that the detectors K 2000 and K 2500 only support the data acquisition via serial port The configuration windows and procedures of the other detectors are identically in each series Detector UVD2 1L Configuration Name UYVD2 1L Interface AN ES Serial Number FO4122700002 Cancel IP Port 10001 Inversion Channel of Signal V Use S N to identify the instrument 1 Channel E Options Iw D2 Lamp on Halogen Lamp Y Axis Units m ll Flow Cell Leak Sensor Sens Medium si Add Info Help UVD 2 1L configuration window Knauer 2520 Configura
34. on this arrow a dialog box for the FC parameters at that selected time will be opened Its appearance will depend on the mode as well as on the selected event Single Event Unconditional FC Parameters at 1 00 min Signal Achon Unconditional Actor Collectio se Collect to EE Shy Recycling Fig 125 FC Parameters Unconditional single event Only the type of action and in case of Collect to the destination vial position is to be selected Next means the next not used vial seen from vial position 1 not from the current collection position Collect to allow defining a vial position for a fraction Only if the fraction collector configuration includes a recycling valve the option Slv Recycling is available Due to that the signal level will not be checked make sure that during the solvent recycling no components will elute After closing the window the parameter will be designated as Timed Peak Recognition Signal Level FC Parameters at 1 00 min Signal Peak Beg Level Write 130 Peak End Level Urata fi 10 Iw Solvent rgcwchre Siet il signal level i we than 50 Archon Achiort Please notice that the parameters vil be used in the Unts selected for De Detectian Channel Canc Fig 126 FC Parameters Signal Level peak recognition 94 Setup and Programming Fraction Collectors In the case of peak recognition mode and event Signal Level the parameter window will enable you to enter the thresho
35. position number will be shown Device Monitor Fraction Collectors Ed Knauer 5 3050 FC m Monito 0 73 min Direct Coritral r Status Collect to A Opershion Waste 7 Sal Signal 7 000 Collect te TC Mest Bes bai ial Wiat 141 Fil Lere rm 0 15 Fr Time fmi 0 15 Fr Vokune fm 0 15 Gear Bach Show Flack Fig 152 Device monitor for the FC 3050 The status and as far as occurring the kind of error is displayed in the headline of the instrument field The monitor is divided in two sections the monitoring and the direct control area The appearance of the window may slightly depend on the type of fraction collector in use Monitor Status The actual operation is displayed It is additionally indicated by the LED color waste gt grey fraction gt green D or solvent recycling gt blue KA Signal The actual value of the controlling detector channel is displayed Vial The actual fraction vial number is displayed Fill Level The filling level of the actual vial is shown in ml It may differ from the fraction volume if consecutive runs are collected to the same vial positions Fr Time The collection time for the actual fraction is displayed Fr Volume The collected volume of the actual fraction Is displayed Direct Control In the direct control mode some operations or selections are possible Operation The operation which shall be performed can be selected from the pull down menu The cho
36. position numbers of the reagent A B vials The selected number will be blue highlighted For the tray 84 3 vials the additional vials 85 87 can be used as Reagent vials If the injection method yl Pick up is selected and one of the additional vials is selected as a transport vial the Reagent vial selection for this vial will be ignored LC Gradient LC Gradient allows the setup of all pump which as not defined as auxiliary pumps If more than one pump is added the pumps will be handled as High Pressure Gradient system HPG whereas the first configured pump is pump A the second configured pump is pump B etc Method Setup My method MODIFIED Common for all detectors Gradient T able Time A D Flow min mL min Standby Flow 1 mL min D o 500 100 0 50 0 El 50 0 Time to Standby 0 min 0 0 4 00 1000 Standby Time 0 min 6 1000 00 7 Idle State C Pump Off mL min 15 ba Initial 1 0 os Standby 0 0 Event Table AS Acquisition PDA Thermostat Integration PDA Method Ce 4 gt t Cancel Apply Report Help LC Gradient Setup Gradient table The gradient table is shaped according the performed pump configuration Entering the gradient time program it is simultaneously displayed with a black line for the total flow and colored areas for the gradient composition The gradient table is a spreadsheet to enter your time program In the first row at time Initial 0 00 min
37. the button Flush Volume The flush volume defines the volume of sample that is used to flush the injection system injection needle and the tube between needle and injection valve before the injection 70 Fig 92 Setup Systems and Instruments Transport Vial for Tray 84 3 vials ul Pick up The tray 84 3 vials includes 3 additional vials with max 10 ml volume that can be used as transport vials for the injection method ul Pick up For each injection with injection method ul Pick up the autosampler aspires one segment of transport liquid before and after the sample volume the so called transport plug The transport plug volume is 2 5 times the tubing volume refer the tab System Settings in the autosampler setup The tubing volume is noted on a label on the tube installed between the injection needle and the injection valve The default tubing volume is 15 ul The filling level of the transport vials will be set to 8000 ul each time the autosampler will be switched on The autosampler calculates then the current filling level after each run Please be sure to fill the transport vials completely because the autosampler also calculates the needle immersion depth basing on the filling level between two different heights If the autosampler calculates that the transport vial is empty the injection and therewith the sequence will be stopped In a method you can only set one transport vial If you need more
38. the Select button to use the selected device information to update the current configuration Device Information Firmware weision Knauer HPLC Box 3 15 Device Information window for the Knauer HPLC Box Serial Number Type in the serial number of the interface you are using Please note that the Manager 5000 5050 IF2 interface can only store a five digit serial number If the serial number is higher than 99999 only the last five digits will be shown example 114355 gt 14355 or ADA120112204 gt 12204 If the serial number has a zero in the second position the Zero will not be shown and the serial number will therefore only have four digits example 104567 gt 4567 or ADA120101204 gt 1204 The Knauer HPLC box cannot store a serial number higher 65536 2 5 If the serial number is higher only the last four digits will be shown 72345 gt 2345 To avoid communication problems Knauer recommends that the serial number be read out using the button when the interface module is connected and switched on Range Select the voltage range for the analog detector output to the interface The choices for the Manager 5000 IF2 are 2 56 V 1 28 V 0 64 V 0 32 V and 0 16 V and for the Knauer HPLC Box 10 V 1 V 0 1 V and 0 01 V Channel count Select the number of channels in use 50 Hz 100 Hz option This option switches the Knauer Interface Device into high sampling rate mode This mode allows for t
39. the tray type from the drop down list The choices are 12 vials 48 vials 96 high 96 low 384 low and 8443 vials The extensions high and low correspond to the position of the well plates Principally it is possible to select different tray types for the left and right side Only the option 384 low for left and 96 high for right is not allowed If the Preparative mode is activated the tray type 12 vials is fixed for both sides If the tray 84 3 vials is selected it is valid for both sides 74 Fig 99 Setup Systems and Instruments The 84 3 vial tray for the autosampler 3950 is supported in firmware version 1 04 or higher A firmware upgrade from former firmware versions is not available Plate Processing Select the order of processing for the vials from the drop down list The choices are Columns and Rows First Dest Vial Enter the position number of the first destination vial The selected number will be green highlighted A destination vial is required if a mixing should be done in a separate vial One destination vial is required for every sample however only the first destination vial position can be specified If more than one destination vial is required these will follow automatically from the first destination vial position Therefore sample and destination vials are both defined as blocks for instance sample vial positions 1 10 destination vial positions 11 20 Reagent A B Enter the
40. this command to select the entire spreadsheet Standby Settings Entering a time for Time to Standby and or Standby Time the execution of this will be added for each run If the Standby is finished the pump will switch to the settings defined by the Idle State Standby Flow ml min Enter the desired standby flow for your pump For a gradient system the Standby will always run with the settings of the Initial line of the pump program Time to Standby min Enter the after run time for changing your pump delivery to the set standby flow Standby Time min Enter the desired time for delivering the set standby flow When elapsed the pump will switch to the idle state and the within defined conditions The described Standby on the LC Gradient tab does not affect the Standby function of some newer Knauer devices ClarityChrom Prep does not support this advanced device feature Idle State The idle state defines the pump status at the end of each run This is also valid for each run inside a sequence Select one of the options Pump off Initial or Standby If Initial is selected the pump will run under the settings of the methods initial line The Standby status is defined above Please note that the Idle state will also be executed if a sequence is running and the system will switch to the next run in the sequence table Options Gradient Options Min Pressure E MPa wv Solvent 1 Max Pressure E MPa W Solvent 2 F G
41. to files ability to modify a calibration integration parameters etc are all determined Each user can adjust the appearance of the station Program Structure and Control Instrument Window Fig 14 The Instrument window measures and evaluates an analysis from a selected chromatograph The window is displayed by clicking on the symbol of the relevant chromatograph in the main ClarityChrom window Depending on the number of configured instruments being used up to four independent Instrument windows can be displayed simultaneously The ClarityChrom station must be set with as many instruments as you want to measure independent analyses Each Instrument window contains an Information Table Status Line and Scheme of data processing Windows are distinguished by line color in the Scheme and by the in strument name in the window header Instrument Displayed method Information table name name rf Instrument 1 Method Method 1 Sek File Monitor Analysis Method Setting W w Window Help DN Chromatogram name Sequence table 5 File Name Instrument 1 201 sae Single run Sample Sample description start stop Sample ID Data acquisition Method Method 1 Measurement conditions lt Mode Single Run method name Monitoring Chromatogram GC LC status display Calibration curve Method setup creation GC LC control meas S i urement A 1 12 min Print conditions Project name For Help press bi e subdirectory Ru
42. windows are displayed Use a name which is unique within the instrument configuration Serial Number If you have read out the device via LAN the serial number has been added automatically Otherwise type in the serial number of the instrument Interface The assistant ASM2 1L supports LAN connection only one LAN port for all 3 devices is required Interhace LAN e IF Pott 1 DIOU Use S N to identify the instrument jw LAN interface dialog The Assistant ASM2 1L can be configured automatically using the button on the configuration window All included device will be recognized and configured Beside this you can search for the devices on right middle and left side separately Configuration Systems and Instruments 49 Fig 63 Leak Sensor Sensitivity Select the leak sensor s sensitivity in accordance with your requirements The choices are Off Low Medium and High Add Info Click the button Add Info to enter more detailed description of the pump If you have already read out the assistant instrument using the l button you may not configure your assistant instrument manually This is the recommended approach The automatic configuration eliminates the risk of a faulty configuration Nevertheless the manual configuration will be described below Left Device Due to that for all three device positions the same devices and settings can be selected the options will only be described for the Left Devi
43. 0000 0 000845 191 503 nm GLP Information Instrunent Power Cycles Instrunent Working Hours D2 Lanp Pover Cycles D2 Lanp Working Hours 0 0 Date of Last VL Calibration 00 00 00 Date of Last Service 00 00 00 Spectrometer information window The PDA detectors S 2850 S 2800 and K 2800 must download their firmware from the computer This will be done if you click on the button or if you open the instrument During the firmware download and the initialization the Configuration Systems and Instruments 37 Fe Fig 50 error LED is flashing this is a normal behavior The firmware download also switch on the detector lamp s If only the device will be switched on the lamp s will not start Y Axis Units Select the type of units to be displayed on the y axis of your chromatogram from the drop down list The choices are AU Absorption Units mAU 0 001 AU or WAU 0 000001 AU Flow Cell Enter a description for the flow cell you are using with your UV detector The description will be printed out when you print the instrument configuration D Lamp Check this box if the detector is equipped with a D lamp Halogen Lamp Check this box if the detector is equipped with a halogen lamp Shutter Control This option is activated by default If this option is chosen the detector will open the shutter only before the run to protect the fiber optics Additionally the dark current will be measured and subtract
44. 2000 K 2001 K 2500 and K 2501 Add Info Click the button Add Info to enter more detailed description of the detector When complete click OK to exit the dialog and return to the system configuration window The OK button is active only after defining the serial port number Configuration Systems and Instruments 33 Fig 45 Fig 46 Fig 47 Configuration Knauer UV Detectors with PDA Functions This group involves the supported detectors K 2600 S 2550 S 2600 Knauer 2550 Setup Geen Name 5 2550 Interface AN ei S Serial Number 44444 Cancel IP Port f 0001 Inversion V Use S N to identify the instrument of Signal Options 1 E Channel 1 R D2 Lamp E Channels 1 CG 8 Halogen Lamp Acquire 3D Data Add Info Y Axis Units m4U e Flow Cal ES p S 2550 configuration window Knauer K 2600 Setup OK Name K 2600 Interface COM1 X ES ESCH Serial Number 133334 ET mu Lance Flow Cell Inversion Z Channels 1 m7 of Signal 1 Channel 1 E Options Iw D2Lamp Halogen Lamp Add Info Acquire 3D Data Help K 2600 configuration window S 2600 Configuration Name s 2600 Intedlace RS 232 sl Serial Number 56701 COMPott COM j e Inversion Channels 2 T of Signal Options 1 Channel 1 8 rates Iw D2 Lamp 42 Channel 2 x 5 Channel Halogen Lemp Iw Shutter Control Iw Acqure 3D Dato YtwisUnte
45. 310 5MB Fig 3 Installation type selection Select the type of installation you prefer It is recommended to use the typical installation A typical installation will install all Knauer devices only some special drivers will not been installed The next window Choose Start Menu Folder proposes the file name ClarityChrom or ClarityChromPrep Of course you can select any other name This name is totally independent on the path for the program files 10 Installation A ClarityChromPrep v 3 0 7 662 Setup Choose Start Menu Folder Choose a Start Menu folder for the ClarityChrom Preparative Gla ri f y e hro m shortcuts a Select the Start Menu folder in which you would like to create the program s shortcuts You can also enter a name to create a new folder larityChromPrep Autostart Catalyst Control Center FreePDF Spiele Symantec Endpoint Protection verwaltung Scom NetCom Zubeh r Fi Do not create shortcuts Knauer Installer Fig 4 Installation Start menu name selection ClarityChromPrep User Code Setup requires you to enter your User code In case of an improper User code the station will start in Demo mode without a possibilty to acquire data and to evalute chromatograms IF you leave the User Code field blank the station will start in 30 Days Trial Mode The Trial mode allows the ClarityChromPrep station to be Fully operational For 100 sessions or 30 days from First opening
46. 5000 5050 interface box IF2 or the Knauer HPLC Box Time Constant Select the time constant for the detector from the drop down list The choices are 0 1 0 2 0 5 1 0 2 0 5 0 and 10 0 sec Smaller time constants are required for high speed analyses A good rule is to select a time constant which is at least one tenth of the peak width at the base of the narrowest peak of interest Sampling rate This is the rate at which data will be sampled by the system For any selected sampling rate the narrowest peak width will be shown for which this sampling rate will be adequate This selection is in Hz data points per second This is the selection for most chromatography applications Click on the down arrow to get a list of the frequencies available for the configuration of your system The choices are 1 2 5 and 10 Hz For a reproducible integration of a peak 20 data points are required in minimum If the 50 Hz Manager 5000 5050 IF2 or 100 Hz Knauer HPLC Box option is enabled also these data rates are available If so only channel 1 is accessible channels 2 4 are disabled 84 Fig 109 Fig 110 Setup Systems and Instruments Channel depending Settings Virtual Detector A virtual detector is not a real instrument but a software tool having special functions For instance it is possible to carry out simulations or corrections of chromatograms with this detector The result will be stored as normally cre
47. Analog Output Mode Eppes Add Info Cancel Help Configuration window for one channel of the Smartline Manager 5000 IF2 Name Enter a descriptive name for the channel Use a name which is unique within the instrument Acquisition Checking this option enables data acquisition feature for this channel Auxiliary In the case that this option is selected the data acquired by this channel will be sent to ClarityChrom as an auxiliary and not be processed as a chromatogram In the opposite case the default setting the data will be sent as a chromatogram The Auxiliary option can be used to acquire parameters such as pressure temperature eic Y Axis Units Fill in the units of your signal to be displayed on the y axis e g microvolt AU MPa etc depending on the detector type An interface device acquires your analog signal and stores it in microvolt If you want to store and to display the signal in different units you must use the correct multiplier Y Axis Multiplier ClarityChrom by default stores a signal in microvolt If you wish to display another unit enter the conversion factor to be applied The following table shows commonly used y axis labels and their corresponding multipliers Y Axis Label Y Axis Multiplier Volt 0 000001 Millivolt 0 001 Microvolt 1 Other Enter a number such that when multiplied by microvolts gives you the desired units For example if you want the label to be units
48. Ih KNALIER Software gt ClarityChrom Version 3 07 Chromatographie Software User Manual V1670 5A ke Channel A II x E Time 30 0263 Minutes Amplitude Volts e CONTENTS Brier DESCHOUOMN ssnin ea a egGebg 5 eegene eae ea ia 5 Computer and Operating System Heourements 6 Metala o a EE 8 PACKING EIS EE 8 eene e E te DEE 8 Program Structure ee K eut EE 13 Main ClarityChrom Window 13 PTS FUMIO WNOOW EE 14 Chromatogram Window n nsesnoaannneneensennnnnnneosrnnnrenrnosrnnnrrrrenssnnnrrrreossnnnnrnreesnennnnn 14 Galibration WINgOW EE 15 SE neie ele EEN 16 Method Setup Dialog EE 17 lee WEE 17 Programi olaf EE 17 Signal WIOMILO MING RE 17 Measurement and Evaluation Parameters ccccccccsseeeeeceeeeeesseeeeeeeeeseeeseeneenens 17 Analysis TEE 18 Analysis let re rue DE 18 Chromatogram Display and Evaluation ccccccccccsseeeeeeeeeeeeeeeeesaeeeeeeeseeneeeeeeeas 19 FAN WOM EEN 19 Configuration Systems and Instruments eeno0aannnnnnnosennnnnnnnossnnnnennrensnnnnrrnnennene 20 Configuration device COMMUNICATION port 22 Connections for device control from computer via LAN a nnanannnnonnnnnannnnnnnnnnennnnne 22 Configuration by downloading from the device 23 Configuration Knauer bumps 24 Gontiguration Knauer Detectors EE 29 Configuration Knauer RI Detectors A 29 Configuration Knauer UV Detectors a0nnnnneeosennnnnnenosennnnr
49. Monitor Direct Control You should switch the lamp on manually before you start the next working session After power up allow 20 minutes for the lamp to stabilize before running samples that may require sensitive measurements Analog output S2600 2800 2850 only An analog output can be activated The recorder range is combined with this option You can define this scale by selecting one from the pull down menu Channel depending Settings Method Setup Default MODIFIED Select Detector 5 2600 Channel 1 Iw Enabled 5 2600 Channel 1 5 2600 Channel 2 5 2600 Channel 3 Detector Setup 5 2600 Channel 4 Acquisition Settings Wavelength T able Bandwidth E nm Time Constant 1 0 ei Sec Sampling Rate 1 Hz Options Iw Autozero at Start Autozero at WL Change ER Lamp OFF at the End of Run Scale 10V itable for Mini Peak width at i Suitable for Minimum Peak Width at Base 0 3330 Min Det Status Description j Status R eady Event Table Measurement Acquisition PDA Integration PDA Method Calculation Advanced Cancel Apply Report Help Acquisition Channel depending Settings In case of multi channel detectors the Wavelength Table is only valid for the selected channel The setup has to be made for all enabled channels separately Setup Systems and Instruments Bandwidth nm all PDA detectors The bandwidth is a measure of the range over which the calculations and measur
50. N b ah ah od mh DEENEN TAKKI KI For Help press F1 0 45 min Running Vial 4 Inj 1 File Name Cal214 Active Auto Sequence window Both an active and a passive operation are possible An active sequence means that the station controls both the start up and duration of individual analyses In a passive sequence they are controlled by the autosampler and chromatograph Active sequence increases the reliability of mutual synchronization between Clarity the autosampler and the chromatograph is required when controlling selected autosamplers through the AS Control module requires the interconnection of a control signal from the ClarityChrom station to the autosampler First Analysis 17 Method Setup Dialog The parameters for determining and describing the conditions for measuring and evaluating are set in the Method Setup dialog These settings are saved in the template method file AS Control GC Control Measurement Acquisition Integration Calculation Fig 19 Method Setup dialog tabs The Method Setup dialog is divided into tabs that are directly accessible from the Instrument window by clicking on one of the following icons een Device Device monitor displaying status information and enabling online Monitor settings Measurement conditions with optional settings for selecting the Measurement duration of a measurement SE Acquisition Parameters
51. PDA Detector Method The method setup PDA window includes a tab as described below and a tab independent button PDA Status PDA Status reads out and displays a status summary Setup Systems and Instruments 89 Fig 120 Fig 121 Select PDA and the field Enabled are not accessible and only for information as soon as a PDA is configured and added to the active instrument PDA Scan Method Setup Default MODIFIED Select PDA E PDA Detector Method Scan Scan nm Range 189 505 nm Start 200 End a50 Bandwidth f Signal Mode Absorbance F PDA Status Description Status Event Table Measurement Acquisition ppa Integration PDA Method Calculation Advanced Cancel Apply Report Help PDA Scan nm Range 189 505 nm This area is used to specify spectral parameters used for your test measurements The range displays the minimal and maximal wavelengths available for the detector If a detector is connected and switched on the physical scan limit of the detector will be shown If not default values for the scan range detector dependent will be displayed Start is the minimal wavelength with a default value of 200 nm End is the upper limit of the scans to be measured with a default value resulting from the configuration Bandwidth is the wave length range that will be used to measure a data point For the spectrum acquisition it is highly recommended to use a bandwid
52. Prep f selected there are additional options You can enter the Vial Volume please be sure to enter the correct volume and setup Tubing and Solvent Peak Recycling Please refer to the chapter Configuration Fraction Collectors for detailed information The Number of Vials is fixed and inaccessible depending on the selected number of positions of the used valve and the Solvent Peak Recycling setup If for another valve in the assistant the option Use as a Fraction Collector is already selected for the next valve an error message The fraction collection option is already selected for another valve will be shown Valco Valve If selected you can choose the device name please refer to the device name section below and the number of position Please be sure to select the correct number of position if you select it manually 2 6 8 10 12 or 16 position can be selected For one valve in the assistant you can check the Use as a Fraction Collector box If selected there are additional options You can enter the Vial Volume please be sure to enter the correct volume and setup Tubing and Solvent Peak Recycling Please refer to the chapter Configuration Fraction Collectors for detailed information The Number of Vials is fixed and inaccessible depending on the selected number of positions of the used valve and the Solvent Peak Recycling setup If for another valve in the assistant the option Use as a Fraction Collect
53. The method name is displayed in the title bar This tab is common for all signals when a multi detector chromatogram is in use Method Description Contains the description of the method Upon selection of a method the description of the method will be displayed in the Description field Column Describes for informational purposes only the column being used Mobile Phase Describes for informational purposes only the mobile phase used Flow Rate Indicates for informational purposes only the mobile phase flow rate Pressure Indicates for informational purposes only the mobile phase pressure Detection Contains for informational purposes only detector characteristics Temperature Describes for informational purposes only temperature parameters Enable Auto stop Sets automatic termination of an analysis after the Run Time If the option is not checked the method can be stopped only manually Run Time Specifies the duration of analysis After the appointed run time has been reached the analysis is automatically terminated and processed The allowed interval time is between 0 2 999 minutes 78 Fe Setup Systems and Instruments A run time must be set for all methods used in an active sequence since the time determines the cycle duration External Start Stop Enables control through the use of an external signal If more than one detector is used the settings are valid for all detectors Start Only The
54. abled the event outputs cannot be controlled anymore they will stay in the last set status Method Setup Default1 Common for all detectors S Output Type 7 LC Gradient Measurement Acquisition PDA Integration PDA Method Calculation AC 4 gt Cancel Apply Report Help Event Table 68 Setup Systems and Instruments It controls up to eight digital outputs for some instrument used to control external devices like valves autosamplers pumps etc as far these instruments are equipped with corresponding connectors Any row of the event table must be filled out strictly from left to right First the event has to be identified by a name Only if this has been done the pull down menus of the following columns become accessible when the field is activated with the cursor Autosamplers Fig 89 The autosampler control is bound with the Sequence It cannot be controlled with a Single Analysis Additionally the option Active Sequence must be activated in Sequence Options This manual only describes the control of the Smartline autosampler 3950 Due to different firmware versions for other autosamplers basing on the same model Autosampler Spark Alias not all features may be supported The settings of the other Spark autosamplers are mostly comparable Knauer Smartline AS 3800 Spark Marathon Knauer Smartline AS 3900 Spark Midas Knauer Smartline AS 3950 Spark Alias firmware de
55. al number gt 50 000 For the older version 2 only the data acquisition via analog input is supported The digital output of the Manager 5000 5050 IF2 is not supported It is automatically used for autozero signal if the Acquisition option for the channel is enabled The configuration process for both Configuration Systems and Instruments 41 Fig 53 Fig 54 types of Knauer Interfaces is the same although some differences exist These differences are specifically mentioned below 5000 Configuration l Box Configuration General Channel 1 Channel d Channel 3 Channel 4 General Channel 1 Channel 2 Channel 3 Channel 4 Mame s5000 Name l Box Serial Fort COM el Interface COM B Serial Number 44444 Serial Number DEG Range A 256W Range 10V Channel Count 4 Channel Count 4 Add Info Add Info Cancel Help Cancel Help Smartline Manager 5000 5050 IF2 Knauer HPLC Box configuration window configuration window The Manager 5050 must be configured as Manager 5000 Name Enter a descriptive name for the Knauer Interface Device This name will appear on the manager tab when the instrument setup or when status windows are displayed Use a name which is unique within the instrument Serial Port Interface Select from the drop down list the serial port number for the communication port on your PC where the Knauer Interface Device is connected Pressing the
56. an interface is selected the OK button is only active after defining the serial port number Configuration Knauer Detectors Fig 38 Fig 39 Configuration Knauer RI Detectors Six Knauer RI Detectors are supported the analytical S 2300 and the preparative S 2400 of the Smartline series as well as the analytical K 2300 2301 and the preparative K 2400 2401 of the WellChrom series The configuration windows and procedures are identically in each series 2300 Configuration Name 2300 Serial Port COMS r Serial Number 12345 As Units WRI Caas AddInfo _ Help S 2300 configuration window K 2301 Configuration Hame K 2301 Setial Port COMI Serial Number 23456 YAris Units RIL Detector Type K 2301 ll Caneel Acte Help K 2301 configuration window Name Enter a descriptive name for the detector This name will appear on the detector tab when the instrument setup or status windows are displayed The default detector name will be suggested automatically by selecting the detector type Use a name which is unique within the instrument Serial Number Type in the serial number of the detector you are using Serial Port Select from the drop down list the serial port number for the communication port on your PC where the instrument is connected 30 Fig 40 Fig 41 Configuration Systems and Instruments Y Axis Units Select the type of units to be displayed
57. and solvent recycling triggered by time volume signal level and or slope Computer and Operating System Requirements The ClarityChrom station runs under Windows 2000 XP Vista 32 and 64 bit and Windows 7 32 and 64 bit in any language version For Windows 2000 XP a Pentium 1500 PC with 512 MB memory will be sufficient For Windows Vista 7 a Pentium 2000 PC with 1 5 GB memory will be sufficient The minimum monitor resolution is 800x600 pixels and 256 colors however we recommend a 1024x768 resolution and 64K colors 16 bit High Color Key Features 7 Verify that you have e A free USB slot for the hardware key depending on the type e ACD ROM drive for software installation e Enough interfaces serial LAN PCI to control all configured instruments 8 Installation Installation Fig 1 The installation procedure of both software packages the analytical and the preparative one are identically Verify that the package is complete according to the packing list Packing List ClarityChrom CD USB hardware key ClarityChrom User Code Safeguard dongle protection ClarityChrom Short Manual Software Installation Installing ClarityChrom is very simple Firstly please start Windows if it is already running close all the user programs currently in use including the antivirus software Do not insert your USB hardware key into the PC slot before the ClarityChrom software is installed Insert the C
58. annel 1 b FO Reset Flow fm rie 7 000 Edi Exp time GE Fraction bid Single Evert Unconditional CC 2 100 Peak Recognition Signal Level 3 Desenptian Staue l Event Table Measurement Acquisition FC Integration PDA Method Calculation Advanced Cancel Apply Repol Help Fraction Collector Settings Flow mL min Enter the pump s flow in mL min to be used for volume and delay calculations This allows calculating the time a peak requires for the way between the detector s flow cell and the fractionation vial also refer the Tubing Parameters in the collector s configuration setup Changes in the flow during a run cannot be calculated Additionally the set flow will be used to calculate the filling level in the fraction vials to prevent that the fractionation vials will be overfilled also refer FC Reset Edit As an alternative for the direct programming of the fraction table or as an additional option click the Edit button to open the FC program editing tool which allows also for graphical programming of fractionation see page 95 FRC Status displays a status summary of the involved collector Fraction Collection Program FC Settings The editing principles are again the same as was described for the gradient table on page 74 Some fields are free for entering data whereas others are either only for information purposes without access or for selecting between possible option
59. as the Purge button on the tab LC Monitor will open the window Set Flow as in Fig 135 Device Monitor 99 Set FLow Total Flow N mL min E E G 50 K Cancel Fig 135 Direct Control of the Pump 1000 The View menu enables to select the view of gradient composition either in or directly in ml The status of the pump is displayed in an additional segment If any error is connected with the pump it will be described in this status line Instrument 1 Device Monitor File Control view Window Help Da 6 amp Always on Top v Fit to Content LC Monitor Compor Customize A Time Tomm Component Flow in Stop Flow B 0 105 3 Purge C 0 175 d En Total Flow mL min 0 700 Pe D 0 035 F Pressure MPa 0 00 Fig 136 Device Monitor Pump 1000 Device Monitor Auxiliary Pump T 5 100 Aux Pump Not Connected Flow ml min HI Fig 137 Device Monitor auxiliary Pump 100 If the Smartline Pump 100 is configured as an auxiliary pump the device monitor differs from the usual of the pumps You are enabled to set a desired flow to stop the flow and to start purging Device Monitor Knauer RI Detectors The status and as far as occurring the kind of error are displayed in the headline of the instrument field Additional the possibility to execute an autozero and flushing of the measurement cell is given by clicking on the appropriate buttons S 2300 Demo Mode 0 000
60. ated chromatogram The virtual detector requires the same general settings as any other detector They have to been set Method Setup C Clarity WORK2Wefault2 Select Detector Virtual Detector v Detector Method Detector settings Argument settings Advanced settings Time Constant 1 0 v Sec Sampling Rate 1 sei Hz Det Status Description Status Event Table Measurement Acquisition PDA Thermostat Integration PDA Method Calculation Advanced Cancel Apply Report Help Acquisition virtual detector settings Time Constant Select the time constant for the detector from the drop down list The choices are 0 0 0 1 0 5 1 0 and 5 0 sec Smaller time constants are required for high speed analyses A good rule is to select a time constant which is at least one tenth of the peak width at the base of the narrowest peak of interest Sampling rate This is the rate at which data will be sampled by the system For any selected sampling rate the narrowest peak width will be shown for which this sampling rate will be adequate This selection is in Hz data points per second This is the selection for most chromatography applications Click on the down arrow to get a list of the frequencies available for the configuration of your system The choices are 1 2 5 10 20 50 and 100 Hz For a reproducible integration of a peak 20 data points better 30 points are required in minimum Method Setup Ethanol in blood
61. bol CA the Off state is represented by an inactive gray LED symbol hall Clicking on the Lamp Off button will after inquiry switch the lamp off and change the button label to Lamp ON The appearance of the HAL lamp control depends on the individual detector and its configuration Device Monitor 101 Autozero Click this button to define the current signal over the specified spectrum as the baseline zero level Digital Channels nm Range 200 450 nm The number of channels that will be activated depends on how many possibilities are given to set new values for the measuring wavelengths Pressing the Set button forces the wavelength change on the detector channels Digital Outs S 2600 only The status of digital outs is indicated The digital outs for S 2600 can be controlled as described on page 65 or during a run from the event table see page 67 Fe The D lamp should be switched on manually from the Direct Control window before starting the next working session After power up allow 30 minutes for the lamp to stabilize before running samples that may require sensitive measurements Click on the e button to open the window with detailed spectrometer information including the GLP information of the PDA detectors Spectrometer Information Spectrometer Identification S 2600 DAD Manufacturer gt Knauer Date of Manufacture 19 08 04 Hardware Version Firmware Version Serial Number Spectral Range Option UY no Ha
62. btained using S N Maximum Flow 10 00 ml min Maximum Pressure 70 00 MPa IP Port H 0001 Use S N to identify the instrument jw F Do not stop pump when closing Clarity Auxiliary Pump Add Info instrument Cancel Help Fig 28 Pump S 1050 configuration window Pump S 1000 Configuration Name E 1000 Gradient Mode LPG D Pei Serial Port COM D Pi Serial Number 55555 Head 10 ml Maximum Flow 9 99 ml min Ausiliary Pump T j Maximum Pressure 40 00 MPa Add Info l Do not stop pump when closing Clarity instrument Cancel Help Fig 29 Pump S 1000 K 1001 K 1000 Prep Pump 1800 configuration window Pump K 501 Configuration Narre Kn Head Di mi deel z 2 May Fleet 9 59 miir Seel Poort coms e d Ma Pressure 40 00 MPa Serial Humber fai GE Auniiary Pump CH cen Fig 30 Pump K 501 K 120 configuration window Configuration Systems and Instruments 25 Fig 32 Fe Pump 100 Configuration Name s 100 i 10m HP Serial Mumbar 10 00 miimin weie ESTA 65 00 MPa 0 10 mirin 40 00 MPa 10 00 emitir IF Poet 110001 Use S M to dently the inctrument Je Avexiliary Pump Adel Inte hap Pump S 100 P2 1S P4 1S configuration window If the pump S 1050 should be controlled by RS 232 in the pump s own setup the Interface must be set to RS232 115200 Please refer to the pump s manual chapte
63. button within the graph area File Analysis Display view Window Help PA fl Ai lt E o D a ei e A Time range 2 min Voltage from 10 to 50 m Detector 1 Detector 2 Subtraction Chromatogram Background Chromatogram 3 0 Time Time 2 983 voltage m 18 281 6 636 Background Chromatogram Subtraction Chromatogram Fig 21 Data Acquisition window Data acquisition can be controlled using following icons amp Single Run d n Sequence Run Abort and Stop First Analysis 19 The measured chromatogram can be compared to an already completed chromatogram e g solvent calibration standard etc There are two ways to do this The File Set Background Chromatogram command selects which chromato gram is to be displayed in the background The Show Subtraction Chromatogram command displays the automatically subtracted chromatogram defined in the Method Setup Measurement dialog The chromatogram in the background will only be displayed during data acquisition Chromatogram Display and Evaluation d CH oi The completed analysis will automatically be displayed in the Chromatogram window An automatic display of the chromatogram can be prevented by switching the symbol next to the Ad icon to Bisefe the symbol The Chromatogram window can be displayed 3 anytime by clicking directly on the Ad icon The required chromatogram can be selected using the ON icon from the Chromatog
64. ce tab Device Name Detector UYD2 15 Knauer Valve Valco Valve Configuration window left device tab device type selection Device Type Select the device type that is installed on the left side in the assistant housing from the drop down menu Pump P2 1S If selected you can choose the device name please refer to the device name section below the gradient type none HPG A HPG B and the pump head 10 ml 50 ml For two pumps the pump heads must be identically Pump P4 1S If selected you can choose the device name please refer to the device name section below the gradient type none HPG A HPG B and the pump head 10 ml 50 ml Due to that the Pump P4 1S is equipped with a pressure sensor you can also select the unit for the pressure monitoring MPa bar psi Detector UVD 2 1S If selected you can choose the device name please refer to the device name section below and the Y Axis Units uAU mAU AU The Flow Cell field allows for entering information regarding the used detector flow cell 50 Configuration Systems and Instruments Knauer Valve If selected you can choose the device name please refer to the device name section below and the number of position Please be sure to select the correct number of position if you select it manually 2 6 12 or 16 position can be selected For one valve in the assistant you can check the Use as a Fraction Collector box only ClarityChrom
65. ch other After power up allow 15 minutes for the electronics to stabilize before running samples that require sensitive measurements If you want your instrument to trigger the start of data acquisition or to perform an autozero you must take care for making the trigger signal and autozero connections The digital inputs and outputs used for the connections are situated on WAGO connectors adjacent to the Integrator Inputs Each of the WAGO connectors corresponds to one of the data acquisition channels To an integrator output of a detector Trigger T Autozero OUT IG AN OU DIG IN RELAY S Se D ANN OS GE a0 M channel 1 RS 232 f channel 2 Ee agention OO Ch nnel 3 a channel A Ka Cable connections to the Manager 5000 or IF2 Trigger Signal Connections If you want your instrument to trigger the start of data acquisition on your ClarityChrom system using the Manager 5000 or IF2 you must connect a trigger signal cable to the Manager 5000 or IF2 The trigger signal should be connected to one of the digital input connectors on the rear panel of the Manager 5000 or IF2 Each of the WAGO connectors corresponds to one of the data acquisition channels The digital input must correspond to that one selected in your instrument configuration for the data acquisition Connect one pole of the trigger cable to one of the connectors labeled DIG IN TTL and the other pole to the connector labeled
66. channel is configured the selector becomes accessible and each detector or channel can be enabled or disabled using the At least one detector channel has to remain enabled therefore you cannot disable the last one Method Setup Default1 Select Detector Iv Detector Method Detector Setup Method Setup Default1 Select Detector IV Detector Method Detector Setup Detector Table Method Setup Default1 Select Detector 5 2600 Channel 1 e Iw Enabled Detector Method Common Detector Settings Channel Setup Acquisition Detector Setup Selector Any involved detector needs its own setup In case of multi channel detectors the channel setup tab is only valid for the selected channel The setup has to be made for all enabled channels The detector common settings are valid for the configured detector and channel independent Setup Systems and Instruments 79 Fig 104 The Select Detector is also responsible for the control of fraction collection After finishing the setup of all involved detectors select this one you want to use for fraction collection control until you will made another choice on the tab for setting up the fraction collector see page 91 From Det reads out the settings activated in the detector Detector S2900 does not require a method setup The conductivity trace will be acquired automatically The temperature and or pH trace must be enabled on the Advanced tab in section Auxilia
67. ct Marker Auxiliary or Alarm from the drop down menu If Auxiliary is selected the event program table is extended by a corresponding column Use Input 1 2 AS Setup Systems and Instruments 71 Fig 93 Fig 94 Select Next Injection Freeze or Stop from the drop down menu for both inputs separately Use SSV If this option field is checked the event program table is extended by the SSV column End Time min If this option field is checked you can define a time interval may be different compare to Analysis Time at which the time base methods will be active Event program Enter the time program for SSV and Auxiliary if selected relays Mixing Methods Method Setup Method 1 Select Sampler IV OS 206GeSanpler Method Mode Time and Temp Inputs and Output Det stem Settings Tray Iw Use Mix Methods Destination Destination Status Tray Temperature 29 C Ready 45 Status KC Gradient Measurement Acquisition Integration PDA Method Calculation Advanced Cancel Send method Report Audit Trail Help Autosampler Mixing Methods Mix methods that use vials for Destination Reagent A and Reagent B can only be programmed if the appropriate vials have been defined in the Tray setup Check the Use Mix Methods option to get access to the fields of this window Select a new Action type from the drop down menu to start a line the choices are Add Mix and Wait Add Autosam
68. desired rack type s from the drop down list s for all positions A D Number of Vials The Vials number will be displayed automatically according to the rack type Vial Volume The rack depended value will be displayed automatically However you are allowed to overwrite it that the volume matches with the volume of your vials The software uses this volume to calculate the filling level in the vials If any fraction is larger than the set volume the next free collection position will be used for continued collection to prevent that a vial will be overfilled Please refer to the chapter Setup and Programming Fraction Collectors for more details Please make sure that in the configuration the same Rack Type is selected as in the collectors setup and the Vial Volume corresponds with the real volume of the vials you use Tubing Parameters Solvent Recycling These buttons will open separate windows which are the same for all fraction collectors and they will be described in the section Configuration General Settings of Fraction Collectors on page 55 The peak recycling function of the FC 3050 is not supported in ClarityChrom Use Diverting Valve Please enable the controlling of the diverting valve otherwise the valve on the collector arm will not switch from Waste to Collect position Configuration Systems and Instruments 59 Fig 75 Fig 76 Configuration MultiValve Fraction Collector Knauer MultiVa
69. dow The design of the device monitor of course depends on the configured system If device parameters will be sent to a device from the Device Monitor the previously loaded method will be deleted from the device To load the method parameters again for a run send the method again by using the Send Method button in the Single Analysis window or the method setup You can also reload the method by clicking File Open Method in the method window If you want to start a sequence the method will always reopened and the method parameters will be sent to the devices 98 Fig 133 Fig 134 Device Monitor Instrument 1 Device Monitor A 0 385 B 0 105 Cc Aus i ey Total Flow mL min 0 700 Cp esu ure MPa 0 00 or 2 Move Tun ff E s1000 v s 2600 Device Monitor The order of instruments displayed in the device monitor can easily adapted to the needs of the user as shown in Fig 134 With a right hand mouse click on the instrument area a menu is opened to move this segment up down to the top or to the bottom Move to the Top Move Up Move Down Move to the Bottom Moving segments of the device monitor Device Monitor Pumps The pump segment of the monitor is only of informative character However you can set changes in direct control via the Control menu This menu enables to shut down the system to stop the solvent delivery and to set the flow and gradient manually LC Control gt Purge as well
70. drop down list the serial port number for the communication port on your PC where the instrument is connected Interface RS 232 S2520 UVD 2 1S only COM Port Select from the drop down list the serial port number for the communication port on your PC where the instrument is connected For the mentioned detectors the maximum data rate using a serial communication port is 10 Hz Fe Detector S 2520 To use the maximum data rate of 80 Hz communication over LAN must be selected as the interface If you use serial communication the 80 Hz data rate will not be available For the Detector S 2550 please refer chapter Configuration Knauer UV Detectors with PDA Functions Configuration Systems and Instruments fe Detectors UVD 2 1S To use the maximum data rate of 50 Hz communication over LAN must be selected as the interface Interface LAN UVD 2 1L S 2520 UVD 2 1S only required for data rates higher 10 Hz IP Address The IP address can be obtained automatically from the device by clicking on the button to search for the device It will not be shown in the IP address field if the option Use S N to identify the instrument is enabled If the device receives the IP address from a DHCP server the IP address may change with every start of the device However the identification via S N allows finding the device also if the IP address is different from the address during the configuration The IP address can only be enter
71. e Detection Channel Tool for Editing of Fraction Collection Program Loaded Data The path for the currently opened chromatogram is displayed in this field Load Click the Load button to open the desired chromatogram to be used for defining the fractionation settings A window which allows for selecting the desired chromatogram will appear 96 Fig 130 Fig 131 Setup and Programming Fraction Collectors Open Chromatoeram C clarity 26x DEMOTWDATA L PRM Chromato Im 18 03 05 2004 11 01 PRM Chromata OOF 113 03 06 2004 11 0 PRM Chiomato O1 08 20071919 26 07 2004 1052 PRM Chiomate 0 03 2007 1913 26 07 2004 1053 File Name 2506mdipm Dome File Type Chromatogiam iles emt 7 LIAl Kaes Method E 06 2004 11 01 16 Recent Linked Calibre e Analpek Data pes Vera ClariteChrom SamplelD SN4616 wine Ranges A ate 1250 m d 10 Hz Sample Sul standard yine mik Tima 100 mp Curent State Mot Signed Hae POA Date Ho Open Chromatogram Window Click on the desired chromatogram If there is more than one signal trace in the data file select only one signal Press the OK button to load the selected chromatogram to the editing tool Moving the cursor inside the graph area will cause it to become a cross hairs In addition a vertical line will appear Depending on the position of the cursor the actual values for Time Amplitude level and Slope will be displayed directly above the graph Time 5 07
72. ect Sampler IV 45 3950 Sampler Method Mode Time and Temp 1Pputs and Outputs Mix Methods System Settings Tray Analysis Time min Injection Mode Flush Yolume pl Transport Vial 1 0 ul Pick up v 30 85 Wach Wash Times never S Preparative Mode The transport plug for the pL pick up injection is 2 5 x needle volume 38 pL status Tray Temperature 29 C Ready AS Status Acquisition Integration PDA Method Calculation Advanced Cancel Send method Report Audit Trail Help Autosampler AS 3950 Mode Time and Temperature ul Pick up tray 84 3 vials Analysis Time min Sets the run time for the autosampler The time should not exceed the measurement time otherwise the system will wait for the next run until the autosampler is ready Injection Mode Select from the pull down menu the mode of injection Selectable are None Partial Loopfill Full Loop and ul Pick up Refer the autosampler manual for detailed information about the injection mode For the autosampler 3950 Alias no transport vial can be defined beside for the tray 84 3 vials The transport liquid for the pl Pick up will be escaped from the wash bottle For the tray 84 3 vials the transport liquid can be escaped from one of the additional vials 85 86 or 87 The filling level for the transport vial will be set to full 8000 ul if the device will be switched on or manually in the Device Monitor if you click on
73. ector Therefore they will be explained together The method setup FC window displays without access for changing the configured fraction collector on top of the tab Det Channel Select from the drop down list of active detector channels that one to be responsible for fractionations Only one detector or one detector channel for multi wavelength detectors can be selected If two detector channels are required it can be realized using a virtual detector refer the chapter for virtual detector setup FC Reset This option must be checked for cumulative collection from repeating runs For each injection the fraction collector starts then with the first programmed fractionation vial All fractions of each injection will be collected in the same vials All information about the before collected fractions will be deleted refer the Show Rack in the collectors Device Monitor This is applicable for all single analysis and for multiple starts of a sequences For all injections made in one sequence run the software stores the filling level to prevent that the fractionation vials will be 92 Fig 123 Setup and Programming Fraction Collectors overfilled If the FC Reset option is disabled each fraction from each injection will be collected in a separate vial In case no empty vial accessible the run will be interrupted Method Setup Noname Select FC Mases aore CO FAC Method FC Settings Det Channel VO Ch
74. ector WellChrom UY Detector WellChrom Rl Detector WellChrom Rl Detector WellChrom UY Detector WellChrom UY Detector WellChrom PDA Detector WellChrom PDA Detector Smartline UV Detector Smartline Rl Detector Smartline Rl Detector Smartline UV Detector Smartline UV detector Smartline multi wavelength detector Smartline PDA Detector Smartline PDA Detector Smartline PDA Detector Smartline conductivity monitor Simulation of data aquisition Smartline column thermostat WellChrom switching valves Azura switching valves switching valve based FC FC with optional cooling Simulation of fraction collection Add Cancel Lists of available control modules filtered by Vendor Knauer Double click on the desired module or select it and press the Add button to open the corresponding configuration window Complete the configuration dialog and press OK to close the window returning to the system configuration window For each device a serial number must be entered otherwise the configuration dialog cannot be finished The Filter allows you to limit the displayed devices As an example the filter Vendor allows you to display only devices from one manufacturer as Knauer 22 Configuration Systems and Instruments Configuration device communication port Some of the devices have two communication ports on the rear panel for controlling the device by the computer One is an RS 232 port also called serial or COM po
75. ed manually if the option Use S N to identify the instrument is disabled The AZURA detector UVD 2 1L only communicate via LAN with ClarityChrom ClarityChromPrep IP Port The IP Port number used for communication can be entered here Please use only port 10001 for Knauer devices Use S N to identify the instrument If this option is disabled the IP address of the device can be entered manually This can be either a numeric IP address or the network name of the device Fe We highly recommend using the S N and not the IP address to identify the instrument because devices may automatically receive a new IP address when reset Options D2 Lamp Halogen Lamp UVD 2 1L S 2520 only Select the build in lamp The detectors can only be equipped with one lamp Leak Sensor Sens UVD 2 1L only Select the leak sensor s sensitivity in accordance with your requirements The choices are Off Low Medium and High Y Axis Units Select the type of units to be displayed on the y axis of your chromatogram from the drop down list For the UV and diode array detectors the choices are AU Absorption Units mAU 0 001 AU or pAU 0 000001 AU Flow Cell Enter a description for the flow cell you are using with your UV detector The description will be printed out when you print the instrument configuration Detector Type WellChrom series only Select the type of the detector you want to configure from the drop down list The choices are K
76. elect another directory Do not install ClarityChrom Preparative into the Windows program Folder when using Microsoft Windows Vista or Windows 7 because the program may not Function properly Destination Folder ClarityChromPrep Space required 310 5MB Space available 72 5GB Fig 2 Installation target selection ClarityChrom proposes the destination directory C ClarityChrom or C ClarityChromPrep Accept this path by clicking on Next If you want to change the path click on Browse and enter the desired path into the Choose Folder window All program files examples and help files will be saved there Fe Especially if you are installing the software with Windows Vista or 7 do not use any subfolder in C Program Files ClarityChrom writes to files stored in sub folders of ClarityChrom or ClarityChromPrep folder respectively Windows Vista and Windows 7 protect Program Files folder from being this operation W ClarityChromPrep v 3 0 7 662 Setup A Choose Components Choose which Features of ClarityChrom Preparative you want to Cla r ty Chr om 2 install P Check the components you want to install and uncheck the components you dont want to install Click Next to continue Select the type of install Typical v Or select the optional Program Files F idee you wish to Help Examples LC Control GC Control H B AS Control ME es 2 D es vn Description Space required
77. ements are used to calculate the intensity for a particular wavelength For example with a 10 nm bandwidth the intensity for 250 nm would be calculated from 245 nm to 255 nm A wider bandwidth results in an increased signal to noise ratio because the multiple measurements cause the random noise to be cancelled out That is if the noise is truly random more measurements will decrease the noise because for each positive noise excursion there is a good likelihood that it will be cancelled by a negative excursion But since the signal is not random it will remain the same over multiple measurements A high bandwidth value will decrease the noise level but also decrease the possible resolution which can be measured The default value is 8 nm but if your peaks have substantially wider UV spectra you can increase the bandwidth value in ClarityChrom to obtain a lower noise level Each discrete channel use its own wavelength program and can be measured with its own bandwidth so you can always obtain the lowest noise conditions while still having a low enough bandwidth to not degrade the resolution measured Wavelength table The wavelength table see Fig 107 for each of the channels is a spreadsheet to enter your time program In the first row at time 0 00 min enter the initial wavelength in nm Then for time in minutes you can define the wavelength for any desired time The minimum time difference between two lines is 0 02 minutes For al
78. ent Method Sending no00nnnnnnnn000anannnennnaa 63 SEUD EE 64 WNGOW EE 64 Instrument WINKOW cceceeeeeceeeeeees 12 96 Jee le aaa 90 UE ee eege eege 101 Interface 25 29 32 34 46 49 51 52 55 COMIQUIALIONS nie EN 39 Jetstream OVEN ee 51 K 120 CGonfgouraton 22 K 2301 CGontouraton 27 K 2501 CGonfguraton 28 29 K 2600 CGontouraton 31 K 501 CGontouraton 22 Key EEN ee 3 LAMP OF EE 80 BEE 20 Local area network ceceeceeeeeeeeeeeeeness 20 LOOD VOUM Oinin e as 71 Main windouw saesenseseseeenenenenennnnnnnennnennne 11 Manager 5000 Winnaar naan E a E A 44 dE Edel EE See AS3800 Measurement acerra ia 76 Parameters ee 16 108 Method SCMNGING aucuricerstunitanecsiwiantsntedaaaeweancteuts 63 CUO E 15 AVALO fe C See AS3900 Missing vials ccccceeeeeeeeeseeeeneeeeeseeees 72 Mixing Method AAA 70 Multi valve fraction collector 57 Needle Heart 71 Operating System Requirements E 5 ACK IMG E 6 PDA CONMQUIATION ME 33 38 Detector VT te ele EEN 8 DIFECE Conto EEN 99 FUNGUO EE 29 31 WIG TINO EE TEE 89 Eiter EE 100 Ee 88 Peak Recognition ccccseeeeeeeeeeeeeeeees 92 Plate processing csccccseseeeeeseeeeeeeees 72 Post run Tunchons 64 Pressure Biel 47 A8 Program E ME 15 SUC LS EE 11 Pump SOMIGUIATION eeben eege 22 FAC AG EE 26 S12 0 6 WEE 73 Beie 105 Reagent EE 73 Recycling valve A 54 Reference Gong 102 Report
79. er Sampler is listed you can use it for a logical trigger In this case a start trigger cable is not required If you dont have a device which can sent a start trigger signal you can add the virtual detector VD to the system If the virtual detector is selected for the start input it will send in a sequence a logical trigger if all devices have sent a Ready status This allows running a sequence without an injection device means without a device which can send the start trigger signal 64 Fig 81 Configuration Systems and Instruments Device Number Ext Start Dig Input E 1000 MM 1 ll Ready Big Output E 1000 Ke E Device Murmber Ext Start Dig Input Sampler 1 MM 1 Ready Dig Output 5 1000 k k ll Device Number Ext Start Dig Input ND MM 1 Ready Dig Output 5 1000 Ke p Devices for the Ext Start Dig Inout S 1000 autosampler virtual detector Without a device selected for the start signal no sequence can be started A number must be selected to activate the start input If a device is selected the start trigger input of the device must be connected with the start trigger cable If the autosampler is selected the trigger cable must not be connected The setting is individual for each ClarityChrom Instrument The Ready Dig Output allows to send a signal from a device e g to control an autosampler which is not supported in ClarityChrom software In Number select t
80. er Preparative Pump 1800 wi K 120 Pump 36 11 MB of space required on the C drive 3316 11 MB of space available on the C drive InstallShield Fig 10 Click on Next to confirm the installation for the listed devices Knauer HPLC System ClarityChrom InstallShield Wizard Ready to Install the Program The wizard is ready to begin installation Click Install to begin the installation Install to c ClarityChrom If you want to review or change any of your installation settings click Back Click Cancel to exit the wizard Fig 11 The next window shows the installation path of the installed ClarityChrom software If you click on Install the installation will start Knauer HPLC System ClarityChrom InstallShield Wizard InstallShield Wizard Complete The InstallShield Wizard has successfully installed Knauer HPLC System ClarityChrom Click Finish to exit the wizard Fig 12 Click on Finish to end the installation Now ClarityChrom is ready to run Fe For running the ClarityChrom software make sure that your USB hardware key is now inserted into the PC slot Program Structure and Control 13 Program Structure and Control Fe This chapter is to give you a brief introduction in the main principles of ClarityChrom For more detailed information refer to the reference and user manuals on the delivered CD The ClarityChrom station has a hierarchical structure After start up the main ClarityChrom
81. ettngs 80 Chromatogram Display and Evaluation aaennnenneennnns 17 WINGOW cececceceeeeececceeeeceeceeeeneeeeeenness 13 Bereet 90 COlMMMOV EN oisit 50 89 103 GOMMOUEATON aeieea i 18 Auto samplers E 49 CoM OVE EEN 50 RI ee 27 PINISMING eea 61 62 63 Fraction CGollechor 55 58 60 Jaeger EE 39 PUMDS anu oration saint ternaleal omecuud anne 22 Switching Valves een000naannnnnneaaanni 52 Virt al We eaaa dee 38 BRATAN BE 33 35 99 RRE RRE 79 Darke CUO EE 102 Data nieeselechon cc ceeeecceececeeeeeeeeeeeees 84 Destination Vials EE 72 Detection CNANNG EE 90 Detector GOMIGUIATI ON EE 27 EREECHEN 44 Method eset ek eee eee 77 Device len Ur e 96 DFG Pe WE EE 20 Diagnostics PDA EE 100 107 Digital elt Me DAC 63 Direct Control Fraction Collector cccceceseeeeeeeees 104 Ee a 99 PUMPS EE 97 Ete EEN 34 Evaluation Garameierg 16 Event E e E 66 External Sig La 77 FG FOXY EE 60 leg WEE 15 FISH VOIUME EG 68 eege 60 Fraction Collection c ccccceeeeees 3 4 19 77 Device Montor 104 NOON AM EE 91 94 Fraction Collector Configuration cc ceeeeeeeeeeeeeeeeeeeeeeeeees 55 EE 90 Gradient MOGO eene 23 SCID ae a aos ietacs 73 Gradient TAC EE 73 Halogen Lomp 33 35 99 Headspace Pressure nonnnnnennnnennnnsennne 72 SEET EE 24 FIP GAB OX EE 39 Tell EE 75 Ver EE 39 40 Injection Mode ooannnnnnonnnnennennnnenennnnennenne 68 Installation eut TC EE 6 Instrum
82. f the option Use S N to identify the instrument is enabled If the device receives the IP address from a DHCP server the IP address may change with every start of the device However the identification via S N allows finding the device also if the IP address is different from the address during the configuration The IP address can only be entered manually if the option Use S N to identify the instrument is disabled Use S N to identify the instrument If this option is disabled the IP address of the device can be entered manually This can be either a numeric IP address or the network name of the device We highly recommend using the S N and not the IP address to identify the instrument because devices may automatically receive a new IP address when reset Interface Press the l button to show the devices connected to the selected interface Click the button Select to select the desired device The configuration information will be shown in the window Press the Accept button to use the read configuration settings in the detector configuration window Spectrometer Information Spectroneter Identification S 2600 DAD Nanufacturer ot KC Knauer Date of Manufacture 16 01 08 Hardvare Version EI Firnyare Version ean bas Fs Serial Nunber wen 83563 Spectral Range Option UV no Halogen Lanp Calibration Filter installed Nunbsr of Pixels Integration Time Range 13 200 ms Coefficient A 131 000000 B 1 44
83. he desired digital output of the device If a sequence is running the signal will be sent if no run is started During a run the signal will not be sent For configured supported autosampler the Ready output is not required Configuration Finishing For general or additional detailed description see the Reference Manual chapter 1 1 3 Configuration Especially obey the options behind the Ki button This button invokes the Instrument Method Sending dialog The method settings program will not be sending to the device if a run is started Therefore in 3 situations the settings can be sending If the instrument will be opened if a new method file will be opened confirming the changes in the method setup dialog Additionally the parameters can be sending manually with a button Send Method available in the Method Setup and Single Analysis window In the setup can be selected if the settings should be send automatically if a window to confirm the method sending should be displayed or if the settings never should be send to the devices Additionally you can select the units for flow pressure and temperature Configuration Systems and Instruments 65 Instrument Method Sending After Each Method Change Send Method to Instrument e Prompt for Confirmation 7 Do Not Send Method to Instrument The method is changed each time after opening the Instrument window using the File Open Method command confirming modification i
84. he use of sampling rates up to either 50 or 100 Hz for the Manager 5000 IF2 and the Knauer HPLC Box respectively The standard sampling rate mode for both Knauer Interface Device types allows use rates up to 10 Hz Note that enabling the high sampling rate mode will allow only working with channel 1 all other channels will be disabled then Additionally the Analog Output control feature will be disabled for this channel Add Info Click the button Add Info to enter more detailed description of the Interface Channel Select the tab for the corresponding channel to switch to channel configuration dialog The number of channel tabs corresponds to the setting in Channel count When complete click OK to exit the dialog and return to the system configuration window The OK button is active only after defining the serial port number Configuration Systems and Instruments 43 Fig 56 Knauer Interfaces channel configuration The following settings must be entered separately for each of the Knauer Interface Device channels Both Smartline Manager 5000 IF2 and Knauer HPLC Box use the same channel configuration procedure Every Knauer Interface Device can be configured to use up to 4 channels Note that each of these channels can be assigned to different ClarityChrom Instruments 5000 Configuration General Channel 1 Name Channel OO i Acquisition Aula YAris Units nm Axis Mutter 0001 Avis Offset In
85. hift and lt CE del gt key at the same time Now the keypad is accessible but the device cannot be controlled by software If you press the function while CONNECT will be displayed you will enter to the COM connection table Changed values must be confirmed by pressing the key lt E gt to move to the next parameter press the function key 6 means 9600 Data Bits BO ee el 0 means no parity Stop Bit The COM settings here and in the software must be identical If e g the parity is set to 0 means no parity it must be set in the detector s setup as well in the software If all parameters have been entered the display shows the message CLOSE KEY Press key lt E gt to leave the setup To switch the device to serial control mode please follow the steps above RF 20A Axs Press the function key lt func gt until the display shows SYSTEM _ Enter to Select Press lt enter gt key Press function key until the display shows RS 232 PARAM _ Enter to select Press lt enter gt key Now the settings for the serial interface can be reviewed and modified if necessary To move to the next parameter press the function key 9600 Data Bits ee 0 means no parity Stop Bit The COM settings here and in the software must be identical If e g the parity is set to 0 means no parity it must be set in the detector s setup as well in the software If all parameters have been reviewed the
86. his spreadsheet to enter your time program The number of columns corresponds to the number of configured valves in the system In the first row at time Init enter the initial position for each valve Then you can define the states of the valves at the given time Method Setup Honame Select Valve La ues cl F Knauer Svatching Valves Method Valves Program Yal Staus Desorption Status Ready Event Table Measurement Zcouibon yabves Integration Calculation Advanced Cancel Apply Report Help Setup window for the Switching Valves Valves Program The choices are BLANK I Inject and L Load for a 2 position valve 1 through 6 for a 6 position valve 1 through 12 for a 12 position valve and 1 through 16 for a 16 position valve The BLANK space means that the valve will not change its state at the given time You can enter the settings either by typing the desired values into the corresponding field or by selecting your choice from the pull down menu which becomes accessible via the button appearing in selected field The principle of editing the valves program table is the same as described for the gradient table on page 74 If you select any field in the comment column the button will appear allowing opening a comment window Comments Valves 3 and 4 for column switching only simultanenously to the same positions Cancel Setup window Knauer Switching Valves Comments
87. hod Valve Setup Time ValveM Comments Initial L 200 Es Status Ready Val Status Event Table LC Gradient LC Measurement Acquisition valves Integration Calculation Advanced Cancel Send method Report Audit Trail Help Setup window for switching valves ValveM of the ASM 2 1L The lt Status gt button opens the status window for the complete ASM 2 1L The status of all configuration modules will be shown Hardware Configuration Assistant Type OGOMZ IL Connection LAN 172 16 5 6 10001 Left Device Knauer Valve Middle Device Knauer Valve Right Device Purp P4 15 Status information Hardware Configuration of the ASM 2 1L valve valve pump If you click on the lt GLP gt button the GLP information for all configured ASM 2 1L modules will be shown Instrument data Seral number Fr 122900001 Firmware version 0 04 Instrument installation date 01 02 2071 00 00 01 Last service date 01 01 2008 00 00 00 Last service code 0 Device working time 479 7 h GLP Information Valvel Valve pe 12P ort Mos Switching cycles 1029 GLP Information ValyveM W alua hina ert PP es GLP information of the ASM 2 1L valve valve pump 88 Setup Systems and Instruments Switching Valves Setup Fig 118 Fig 119 The method setup Valves window includes one tab as described below and a tab independent button Val Status Val Status reads out and displays a status summary Use t
88. ication is 10 Hz Fe Detector S 2550 To use the maximum data rate of 80 Hz only one detector channel should be configured and communication over LAN should be selected as the interface If you use serial communication and or configure the detector to use more than one channel even with LAN only 10 Hz data rate is available For serial communication in the detector s own setup the Interface must be set to RS232 115200 Fe The detector S 2600 must be set in its own setup in CONTROL to RS232 binary baud rate 115200 The LAN or USB connector is internally not connected Interface LAN S 2550 only required for data rates higher 10 Hz IP Address The IP address can be obtained automatically from the device by clicking on the l button to search for the device It will not be shown in the IP address field if the option Use S N to identify the instrument is enabled If the device receives the IP address from a DHCP server the IP address may change with every start of the device However the identification via S N allows finding the device also if the IP address is different from the address during the configuration The IP address can only be entered manually if the option Use S N to identify the instrument is disabled If you want to acquire data from the detector S 2550 with 80 Hz LAN must be selected as the interface IP Port The IP Port number used for communication can be entered here Please use only p
89. ices are waste and solvent recycling It will be executed by clicking on the Set button Collect to The operation fraction collection can be initiated here The destination vial is either defined as next or in case of collect to by its number The collection starts by clicking on the Go button Pressing the question mark i button will display a window with instrument information Fe The actual operation Collect Recycling or Waste depends on the last click on either the Set or Go button All operations started in the direct control mode are performed unconditioned as timed events For consecutive conditioned runs the values for level slope and the expected interval will not be stored Clear Rack Click the Clear Rack button to clear the occupied positions in the rack 106 Fig 153 Device Monitor Clear Rack will delete all information of collected fraction from the rack Make sure that all vials have been emptied to prevent that they will be overfilled or different fractions will be mixed in a vial Show Rack Click the Show Rack button to display the rack Rack View The type of rack displayed corresponds to the configured one The shape of the rack view for the Multi valve fraction collector also depends on the configured valves If different valves 6 12 or 16 positions are combined the graphical separation may not be shown The number of displayed positions also may succeed the actual existing number of collectio
90. ices may automatically receive a new IP address when reset Interface Click the button to get a list of all devices of the selected type connected to the selected Interface The Device Information window Fig 33 allows for the configuration information to be read directly from the connected devices Click on a device and on the Select button to copy the configuration information from the device into the configuration window Interface JetStream Oven Smartline column thermostat Select from the drop down list the serial port number for the communication port on your PC where the thermostat is connected 54 Configuration Systems and Instruments Serial Number Type in the serial number of the oven you are using When complete click OK to exit the dialog and return to the system configuration window The OK button is active only after defining the serial port number Configuration Switching Valves Fig 69 The Switching Valves icon refers to a group of the switching valves each of them must be configured before using in an instrument method Double click the icon and complete the configuration dialog Knauer Switching Valves Configuration Name Valves 1 Number of Valves 2 ai Add Info Valve 1 Valve 2 Valve Name Valve 1 Interface RS 232 Serial Number ois COM Port COM14 Me E Valve Type 6 positions S Valve Add Info ok Gei Configuration window for the Switch
91. ing Valves Name Enter a descriptive name for the selected valve This name will appear on the valves tab when the instrument setup or status windows are displayed Use a name which is unique within the instrument Number of Valves Enter the number of valves used in a group The maximum possible number is 12 Add Info Click the Add Info button to enter more detailed description of the valve group Enter additional information or comments to be printed out when you print the instrument configuration The following settings must be entered separately for each of the valves Valve Name Enter a descriptive name for the selected valve This name will appear on the valves tab when the instrument setup or status windows are displayed Use a name which is unique within the instrument Automatically a unique name will be given Valve 1 Valve 2 A Knauer valve drive recognizes automatically if it will be controlled via RS 232 or LAN it must not be switched between RS 232 or LAN control mode Interface RS 232 COM Port Select from the drop down list the serial port number for the communication port on your PC where the instrument is connected Interface LAN IP Address The IP address can be obtained automatically from the device by clicking on the button to search for the device It will not be shown in the IP address field if the option Use S N to identify the instrument is enabled If the device receives
92. ion curve Fig 17 Program Structure and Control ETHANOL 2 308 min Signal 1 22 5900 0 4000 0 0177 1 49 2673 0 9000 0 0183 iV 70 5179 1 4000 0 0199 1 Response Base PA e Compound Type Ordr tsi Curve Fit Type Linear Origin Curve passes through Ori v Weighting Method None el Equation Y 1 14649 Correlation Factor 0 9990433 TLS PIN Compounds ETHANOL rang 7 Calibration curve window Sequence Window Fig 18 LACH The Sequence Window opened using the icon from the Instrument window is used to define the sequential measurement of multiple samples SS 7 AA Maaf Measurements are done individually in the table by row Each row can define the way multiple samples are measured using the same method for multiple samples and or by taking multiple injections of one sample My GC Sequence Ethanol in blood File Edit Sequence view Window Help ft fy Ax Le E wa A aH ge o DF TO Sample Sample ISTD Sample Inj Vo File Method Report ohier E E 2 000 Blank Ethanol in blood 2 000 Cal204 1 Ethanol in blood Calibration 2 000 Cal209 2 Ethanol in blood Calibration 2 000 Cal214 3 Ethanol in blood Calibration 2 000 Cal219 4 Ethanol in blood Calibration 2 000 Cal224 5 Ethanol in blood Calibration 2 000 Cal226 6 Ethanol in blood Calibration 2 000 Pers A Ethanol in blood Chromatogram 2 000 PersO1B Ethanol in blood Chromatogram KIKIKI KI 1 2 3 4 5 6 6 7 8 OO N OO Ou De UOU
93. ionally the tray thermostat can be switched On or Off and a needle wash procedure can be started The tray can be moved position by position by clicking on the Move Tray button If during a run the autosampler s Analysis time is running out refer method setup autosampler tab Mode Time and Temp the Move Tray button will become active This allows for add or remove vials during a running the sequence A mouse click on the 7 button shows a window with the instrument s serial number name and firmware version A mouse click on the gt button allows direct control access for the syringe tray and valve position If the tray 84 3 vials tray is selected the filling level of the transport EL Device Monitor Column Ovens Oven 5 4050 Toven 39 6 C Tcooler 15 2 C oO Temperature TL ap Set Digital Dute E vents A D Cooler Temperature UL am Set Instrument formations BEE 2 Leakage Alarm Level St o Se Set Temperature Control Off Device Monitor Column Oven S 4050 F Jetstream Oven Demo Mode 300 C EA Temperate Cr EN Se 7 For Help pess Fi Device Monitor Column Oven Jet Stream The status and as far as occurring the kind of error is displayed in the headline of the instrument field Temperature C The desired oven temperature can be entered and send to the oven by pressing the Set button Cooler Temperature C S 4050 only The desired cooler tempera
94. it from all measured spectra It is recommended to leave this option always enabled Acquire 3D Data Check the Acquire 3D Data option to utilize the full spectral functionality of this detector If the Acquire 3D Data option is selected you will need additional PDA license option to be able to work with the spectral data If this option is not selected the detector will be used as a multi channel detector Add Info Click the button Add Info to enter more detailed description of the detector When complete click OK to exit the dialog and return to the system configuration window An Instrument 1 x Instrument If you use a PDA detector select the Instrument Type Instrument Type LC PDA or GPC PDA if also the GPC license option CG E is used from the drop down menu for you instrument Otherwise the PDA detector cannot be added to an instrument DHA SimDis Configuration Spectrofluorometric Detector RF 10Axl RF 20A Axs Shimadzu RE 10Axl Configuration Hame RFA UE Serial Number 76543 Seral Fort COM MM gt Y Ass Units Add Info Flow Cell RF 10Ax configuration window 38 Fig 51 Configuration Systems and Instruments The driver Shimadzu spectrofluorometric detector model RF 20A Axs allows the identical functionality as for the model RF 10AxlI driver As of May 2011 the firmware version 0 90 is required The RF 20A Axs is limited on the functionalit
95. jee Preparative Pump 1800 P2 1L K 1001 K 1000 K 501 K 120 pumps Fig 36 Available pump heads Once you have chosen the pump head the related information about material maximal flow and maximal pressure is displayed automatically Auxiliary Pump Check this to configure the pump as auxiliary otherwise the pump will be configured as a solvent pump If more than one solvent pump is configured all those pumps will be used in a HPG Do not stop pump when closing Clarity instrument Check this to run the pump continuously also if the instrument is closed If it is required to run the pump continuously e g if a buffer is used check this box If the instrument will be closed the pump will run with the last idle flow and still show on the display that it remains in remote slave mode Leak Sensor Sens P2 1L only Select the leak sensor s sensitivity in accordance with your requirements The choices are Off Low Medium and High Add Info Click the button Add Info to enter more detailed information regarding the pump Configuration Systems and Instruments 29 Fig 37 Additional Information Flow in the system iz limited to 5ml due to security reasons pump setup Cancel Additional information box Enter additional information or comments to be printed out when you print the instrument configuration When complete click OK to exit the dialog and return to the system configuration window If a serial port as
96. l PDA detectors the selected wave length the bandwidth must be in the scan range selected in the PDA settings An example If the scan range is 200 400 nm and the bandwidth is 8 nm the maximum allowed wave length is 396 nm The principle of editing the wavelength table is the same as described for the gradient table on page 74 Acquisition S 2900 Monitor Method The S 2900 conductivity monitor does not require a setup window The additional settings pH and temperature can be enabled on the Advanced tab in the Auxiliary signal table Channel depending Settings User Defined Detector To control any user defined detector not directly controllable by ClarityChrom the Manager 5000 5050 IF2 or Knauer HPLC Box must be configured This has to be considered in the channel depending settings Setup Systems and Instruments 83 Fig 108 Method Setup C Clarity WORK2WDefault 2 Select Detector Channel 1 v Detector Method Interface Channel Settings Time Constant 10 el Sec Sampling Rate 1 Hz Suitable for Minimum Peak Width at Base of 0 3330 Min Det Status Description Status Event Table Measurement Acquisition PDA Integration PDA Method Calculation Advanced Cancel Apply Report Help Acquisition user defined detector settings Channel Enabled on Check the Enabled on box to enable data acquisition for the user defined detector connected via either the Smartline Manager
97. larityChrom CD into the CD ROM drive After a period of about 30 s the installation menu will appear i KNALUER Km Sr ClarityChrom gt Install ClarityChrom Software Main installation gt Install Updates Installs latest updates for ClarityChrom required gt Release Notes Requires Adobe Acrobat Reader gt Online Manual Requires Adobe Acrobat Reader gt Visit the KNAUER Website gt Browse the CD gt Exit 2008 Wissenschaftliche Geratebau Dr Ing Herbert Knauer GmbH Installation main menu To install ClarityChrom click to Install ClarityChrom Software The first window of the software installation assistant will open In case your CD ROM drive has no AutoPlay please start using the Windows Explorer the program ClarityChrom Disk1 Setup exe on your CD by double clicking on it You will then be guided through the installation By clicking on Next gt you achieve the next window By clicking on lt Back the previous window will pop up again At any time you can interrupt the installation by clicking on Cancel W ClarityChromPrep v 3 0 7 662 Setup Installation 9 IE ClarityChromPrep v 3 0 5 505 Setup ae Choose Install Location Choose the folder in which to install ClarityChrom Preparative Gla 71 ty Chr omz Setup will install ClarityChrom Preparative in the following directory To install to this directory click Next To install to a different directory click Browse and s
98. ld values for the peak at the beginning and at the end The action is set to collect to next Check the solvent recycling option available only with configured recycling valve if desired and enter the signal level for After closing the window the parameter will be designated as L without leading sign Peak Recognition Signal Slope FC Parameters at 1 00 min Signal Iw Solvent recyclin Peak Beg Slope Units min Je el Start if signal level is lower than 20 Peak End Slope Units min D Action Action Please notice that the parameters will i d be used in the Units selected for the Detection Channel Cancel Fig 127 FC Parameters Signal Slope peak recognition When using the Signal Slope instead of the Signal Level the settings are made similarly The only difference is that the slope values defined as signal difference per 1 sec are to be used as thresholds Only positive values of slope are accepted in this window If a recycling valve was configured the solvent recycling can be activated After closing the window the parameter will be designated as S Peak Recognition Signal Level Slope FC Parameters at 1 00 min Signal Peak Beg Level Writs 130 Ce AND C OF Peak Beg Slope Dletzdrmgpt f2 Peak End Level Lira 10 CG AND OF Peak End Slope Dle zdegnt fis Iw Sokent recycling Siet d signal level is lower than en Achon amp chore Please nolice that lhe parameters wall be uad i
99. logen Lamp Calibration Filter gt installed Number of Pixels gt 256 Integration Time Range 13 200 ms Coefficient A gt 191 904358 B ees ket SCC 0 000833 192 504 nm GLP Information Instrument Power Cycles Instrument Working Hours D2 Lamp Power Cycles D2 Lamp Working Hours HAL Lamp Power Cycles HAL Lamp Working Hours Date of Last WL Calibration Date of Last Service Fig 144 Spectrometer information window S 2600 Diagnostics Knauer PDA Detectors Click on jem button to use the diagnostic features of the software The Diagnostics window appears which allows you to access information and to control important parameters and modules of the device 102 Fig 145 Device Monitor Diagnostics Integration Time Range 13 200 100 RH mS ec Scan nm Il enge 194 509 nm Close Shutter Stark 300 End 500 Bandwidth 1 Mode At ti z ol la Set Filter OF Signal mA Dark Signal Rezet Dark Set Reference Spectrum 400 Wavelength nm Spectrometer information window S 2600 Integration Time Range 13 200 msec The integration time is the time interval when the detector accumulates light to receive one spectrum This value is a measure of the intensity of light originating from a lamp The more light that passes the optical scheme and the cell the shorter the integration time will be The integration time depends also on the specific spectral interval Scan selected fo
100. lve FC Setup FC Name Multivalve FC Tubing Number of Valves 2 Vial Volume ml 1100 Number of Vials 21 Valve 8 Valve 2 Serial Port COMI e ks Valve Type 12 positions Valve Add Info _ Add Info Help Multi valve fraction collector configuration window Name Enter a descriptive name for the collector This name will appear on the fraction collector tab when the instrument setup or status windows are displayed Use a name which is unique within the instrument Tubing This button will open a separate window which is the same for all fraction collectors and it will be described in the section Configuration General Settings of Fraction Collectors on page 55 However you cannot define the tubing parameters for each included valve One setting will be valid for all cascaded valves Solvent Recycling With the Knauer MultiValve fraction collector an additional recycling valve is not necessary The recycling port will be defined automatically as the last 2 free ports of the valve if the option Use Own Ports for Recycling is enabled If you wants to use a separate valve for recycling enable the option Use Recycling Valve and select the COM port the recycling valve is connected Please note that at least 2 ports will be reserved for the recycling although only one port is required for solvent recycling Solvent Recycling Recycling Volumetic Delay e m m SR m Sol recycling part 22
101. m Air Segment Check the air segment box to enable the use of an air segment between the sample and the wash solvent to reduce the amount of flush volume Reset Output Check this option if the outputs should be reset to the default settings after finishing the last series Skip missing vials Activate if the autosampler shall not stop if a vial is not found but continue with the next programmed vial Deactivate to stop the autosampler in case a vial is not found Headspace Pressure Check the Headspace Pressure box to enable the use of headspace pressure in combination with the syringe to transport sample to the loop The compressor will always be used during a wash procedure The accuracy and reproducibility of the autosampler may decrease if headspace pressure is switched off Tray Method Setup Method 1 Select Sampler E 45 3950 Sampler Method Mode Time and Temp Inputs and Outputs Mix Methods System ee se Akoa First Dest Vial CH 0 9 999 066000 48 vials v Ce Bigqo000e 600000 Right Tray Type Reagent A 48 vials Plate Processing Reagent B Rows E Status Tray Temperature 29 C Ready 45 Status Measurement Acquisition Integration PDA Method Calculation Advanced Cancel Send method Report Audit Trail Help Autosampler Tray The appearance of the tray tab depends of course on the configured autosampler Here the AS 3950 is described as example Left Right Tray Type Select
102. m the flow values entered by the user Detector The detector is controlled by setting analog output voltage automatically recalculated from the wavelength values entered by the user Options Click the button Options to open the analog output control mode seitings dialog Analog output control mode settings LC Pump Options Flo Control Factor 13 TY a gt 1 30 mimin Maemumn Flia E mi mr Aumgkarm Pump M Use sequisiion channel to collect pressure dats cnet AO control options for LC Pump mode Configuration Systems and Instruments 45 Fig 58 Detector Options YWavelenglh Control Factor E 1Y gt 120mm Minimum ail 200 nn Mavetmum wl 500 nin AO control options for Detector mode Factor for the LC Pump mode Enter the conversion factor used for recalculation of the flow values into the corresponding analog output voltage Maximum Flow Enter the maximum flow rate for the configured pump Auxiliary Pump Check this option to configure the pump as an auxiliary pump By default the pump is configured as a part of ClarityChrom LC Gradient system Use acquisition channel to collect pressure data Check this option in the case that the analog input of this channel acquisition part should be used to collect pressure data In this case acquired data will be sent to ClarityChrom as pressure data from this pump In case that the pump is configured to collect pressure data please check that the
103. me min UK Cancel Virtual detector settings data file selection The very last file selection will be displayed You can either enter a new file or you can use the Browse button To avoid possible signal distortion the Y Axis multiplier in the configuration of the virtual detector must be equal to that of the selected data file ffnen 4 Arbeitsplatz H 3 Diskette A z Lokaler Datentr ger C o Clarity D ateiname Dateityp A Files Abbrechen Virtual detector settings data file selection External Source The Settings button also becomes accessible Clicking on it the following dialog box appears 86 Fig 113 Fig 114 Setup Systems and Instruments Select Channel Virtual detector settings channel selection You can select from the pull down menu one of the detectors or detector channels accessible in the configured system To avoid possible signal distortion the Y Axis multiplier in the configuration of the virtual detector must be equal to that of the selected User Detector Argument Y This option becomes accessible if the Argument X field is activated The setting dialogs for Y are than the same as for X Formula X Y You can either fill in the desired formula or you can select it from the pull down menu providing all of the formulas used before Formula possibilities Following functions constants and operations will be supported Functions SIN
104. min Amplitude 31286 443 Slope 886 000 100000 50000 J ji Opened chromatogram in setup window Move the cursor to the desired position and double click with pressed Ctrl key In the fraction collection program a new line will be created and the parameter window for this line will contain the current time level and slope values By default the peak recognition mode with the signal level slope event will be suggested Complete the parameter settings as necessary and click to another line in the fraction table to apply the settings Repeat this procedure as often as required to program additional events You can also program the collection events directly in the table The parameters for the selected event you can enter in the right handed part of the window The options are identically with the table programming as described above When complete click OK to exit the dialog and return to the method setup window Device Monitor 97 Device Monitor Fig 132 Clicking on the icon of the instrument window will open the device monitor displaying status information and enabling online settings r My LC Method Default1 MODIFIED aka File Monitor Analysis Method Setting View Window Help DN File Mame hy LO 2 25 August 2006 Sample Sample ID Method Default MODIFIED Mode Single Run K i ee SCH Waiting g 4 k E g ST A For Help press Fl Project Work Instrument win
105. mmunication ID Enter or select from the drop down list the ID number for the autosampler in use Without the correct ID the software cannot establish communication with the autosampler For the autosampler AS 3950 it is inaccessible fixed at 61 Serial Number AS 3950 only Type in the serial number of the interface you are using If you use the l button the serial number will be read out from the device automatically Sequence Mode Knauer Optimas AS 3800 Marathon AS 3900 Midas only The sequence mode allows advanced wash features for the autosampler If this option is enabled in the method settings the wash modes between series between vials and between injections become available When complete click OK to exit the dialog and return to the system configuration window Configuration Thermostats Column Ovens Fig 66 Oven S 4050 Configuration Name Oven 4050 Interface RS 232 sl Serial Number COM Port COM1 Cancel Add Info Help Configuration windows for the Smartline Oven 4050 Configuration Systems and Instruments 53 Fig 67 Fig 68 JetStream Oven Configuration OF Mame J etStream Oven Cancel Interface COMI z Genal Number 34567 Add lnfo Help Configuration windows for the Jetstream Oven Name Enter a descriptive name for the oven Use a name which is unique within the instrument Interface Smartline Oven 4050 The S 4050 suppor
106. n information from the device into the configuration window Serial Number If the configuration information from the pump have been transferred using the button the serial number field has been filled automatically Otherwise type in the serial number of the pump you are using The serial number of network controlled pumps is used for the device identification and addressing during serial communications This serial number should match the serial number of the defined pump For pumps S 1000 K 1001 K 1000 and Preparative Pump 1800 at least five 28 Configuration Systems and Instruments digit numbers has to be entered Otherwise you will get an error message when the window shall be closed CH The Serial number is invalid Fig 35 Error message serial number out of range for network controlled pumps Head If the configuration information from the pump have been transferred using the button the correct pump head has been selected automatically Otherwise select the type of pump head you are using from the drop down list Head 10 ml HP si Head E SI Ma Flow S rn Nam Flew Maw Presture 65 00 WPa 010 rimin Maxum Pressure 15 00 MPa 40 00 WPa 10 00 mmm S 1050 S 1000 pump S 100 P2 1S P4 1S 10P 20P pump Head 10 m chee el Ma Flow 10 ml steel i 50 ml Heal Head 1000 ml dei Hlas Pressure 10 mil PEEK kutin Pave 50 ml PEER Maamsn Presne 10 ml Geer 50 ml ciam 10 ml titan 50 ml
107. n positions due to the rectangular arrangement E Vial Table luet 1 0 37 1 32 min 0 95 mi A we jet onl Get ve jet wed Or 1 SE Se Amt ent mut dall pat l t mn h w sea C aC semi O vm CH wog Y domo eC ae ao a y d im EE On asa O ee O oe ee os oe as O i j ass O ce 9 s ee es ce O ae O m er 3 or ee oe eo C EE Ce ETE C EE o Clan Cla west ws OJ Dee os CH sc we Y ons C E sO O t E een Ee mag ama CH 263 Ges e mat joa e JC ma wna C SE SE EES it am CH ae 4 ari a mit II CH vi O iD CH e 3 DI C wi C iHi C me 2e eC set set ise ae ant eut mut n SOte mut mut EE EE ag 19 C3 19 CO3 19 C9 ra O 0a O a CY 23 ace E Hee EH Ee Ee ET Mee a zole Oa Oa Oe Ofe Oe DeO T O om CH om C 2 Y a om C a CH ELEL EL EE E Ee we Y 6 5 108 C9 15 CO ars O a C s CD 25 CH 205 CH 205 CH 2s CH ss e CH ons CO EE EE EE mt ina 18 C mim See Ee E Oe E CH sat O as CH E w gl wmCHecChumCamCmcCHlecimcn an CH 201 CH 201 CH zm Case Can OC Fraction Collector vial table All already used vials will appear highlighted green Moving the cursor across the rack will change the upper descriptive line There the vial number the begin and end of collection and the collected volume in form of Vial 1 0 37
108. n the Method Setup dialog Instrument Method Units Flow Rate Units rien Y Pressure Units MPa v Temperature Units C en lm Fig 82 Instrument Method Sending Fe The setting is common to all instruments GC LC AS devices attached to the same ClarityChrom Instrument Digital Outputs Digital outputs are present on the rear panel of some devices and can be used for custom operations Clicking on the icon in the main window will open the instrument selection If the configuration only includes one device with digital outputs the instrument selection will not be shown Select Control coca Fig 83 Instrument selection for digital outouts Select the desired device to open the corresponding digital output dialog window Digital Outputs of 1000 SN 40004 Output Initial Skate Current Descriptions na State L klo jam O EO en CEA CEA Jae oa boa m CEMA EA Em lt 0 4 0 fe o 0 pa Oi 0 pr Close Fig 84 Setting of digital outouts 66 Setup Systems and Instruments Setup Systems and Instruments Fig 85 Fig 86 This Chapter describes the commands of the Instrument window the fundamental window for chromatogram measurement and evaluation The station allows for up to four Instrument windows to be displayed at one time The number of displayed instruments may exceed the number of instruments actually purchased but the excess instrument
109. n the Units selected for tie Detection Chance Fig 128 FC Parameters Signal Level Slope peak recognition Peak recognition using the slope and the level is performed similarly as described above The only difference is that both the level and slope values are to be used as thresholds In addition one must select either the AND option meaning that both conditions must been exceeded or the OR option meaning that only one of these conditions must be exceeded This can be defined differently for the ascending and descending flange of the peak After closing the window the parameter will be designated as for example Lb and Sb Le and Se Solvent Recycling Check this option if a solvent recycling valve is configured and you wants to perform solvent recycling Enter the signal threshold for the solvent recycling option Recycling will be activated if no peak is currently collected and the signal is lower than the threshold Action This selection as far as accessible is to be made in all parameter dialog windows independent of the set mode unconditional signal level The choices are Waste Collect To Solvent Recycling Setup and Programming Fraction Collectors 95 Action selection Waste The eluate will be directed to waste Action selection Collect To The eluate will be directed to the set position Action selection Solvent Recycling The eluate will be collected in a recycling container because its purity is better
110. n the detector Y Axis Units Select the type of units to be displayed on the y axis of your chromatogram from the drop down list There is only one choice for the spectrofluorometric detectors arbitrary units a u Flow Cell Enter a description for the flow cell you are using with your spectrofluorometric detector The description will be printed out when you print the instrument configuration Add Info Click the button Add Info to enter more detailed description of the detector Configuration Systems and Instruments 39 Fe When complete click OK to exit the dialog and return to the system configuration window The OK button is active only after defining the serial port number The detectors RF 10Axl and RF 20A Axs must be switched into serial mode Please refer the detector s manual RF 10Axl gt pages 7 18 through 7 25 for detailed information The detector RF 20A Axs is set to serial mode if Knauer has made the firmware update to version 0 90 How to switch the spectrofluorometric detector into serial mode RF 10Axl Press the function key lt func gt on the detector s keypad as often as RS232C will be displayed Confirm with lt E gt key The display will show CONNECT also confirm with lt E gt key Now the device can be controlled by the software but the keypad is blocked Also if you switch the device off and on again it still remains in serial mode To leave the serial mode press the lt s
111. n time PP i es User name Status line Analysis monitoring Analysis evaluation conditions Instrument window All dialogs that enable the performance of actions that may be required on a given instrument can easily be displayed from the Instrument window by clicking the corresponding icons in the Scheme or using commands from the menu Sometimes it might happen that you cannot open any other window or the station will not react to your commands This happens when working with what are known as modal windows A modal window is a window that while opened forbids work to be performed in any other window particularly windows which are used to select files set parameters etc Chromatogram Window d CH The Chromatogram window is the central window opened by chromatograms Use the icon to open one of the chromato om E clicking the A7 icon for displaying modifying and evaluating AAKE grams The results table for a selected chromatogram can be found in the Results tab If it is not automatically displayed click on the label of the Results tab in the bottom left corner of the window or use the Results Result Table command Program Structure and Control 15 Selection of displayed Switch to other station Work with multiple Custom chromatogram windows detectors chromatograms Jabel My Gl Chromatogram 2506multi RI 1 10 2003 10 01 24 Recent Linked Calibration File Adit Display Chromatogram Me
112. nauer autosamplers are to be controlled similar to the Spark autosamplers Knauer Smartline AS 3800 Spark Marathon Knauer Smartline AS 3900 Spark Midas Knauer Smartline AS 3950 Spark Alias firmware dependent The Spark Optimas autosampler is not equipped with a serial port while the Knauer Optimas can be controlled via serial port There exist different autosamplers based on the Spark Alias Due to this it is not guaranteed that all options of all models are supported The configuration windows for the Spark and Knauer autosamplers are all practically the same Knauer AS 3950 Spark Alias Configuration KE Seral Humber EES Communication ID Cancel Interface w Use GAN to identify the instrument Configuration window for the autosampler AS 3950 Alias Spark Midas Setup e Configuration window for the autosampler AS 3900 Midas equal to Knauer Optimas The autosampler S 3950 Alias can be equipped with a LAN port or with a serial port The second connector which looks like a 9 pin serial port and labeled with I O is to be connected with the I O cable for separate non software based external control and trigger cable Interface RS 232 S 3950 ALIAS only COM Port Select from the drop down list the serial port number for the communication port on your PC where the instrument is connected Interface LAN S 3950 ALIAS only IP Address The IP address can be obtained automatically fr
113. nformation and enabling online settings as described in the section Device Monitor from page 91 When the sequence is running the post run checkboxes will be locked to the state corresponding to the setting on the currently running row see the Reference Manual chapter 6 Sequence section Post run functions Depending on the icon you have clicked on the opened window seems to appear different however all of the possibilities include the same tabs at the bottom of the Window The indicated tabs correspond to the configuration of the opened method Independent on the displayed tab are the placed the buttons OK Cancel Apply Report and Help at the bottom of the window Event Table LC Gradient Measurement Acquisition Integration PEA Method Calculation Advanced Cancel Send method Report Audit Trail Help Bottom of the method setup window OK Accepts the entered settings and closes the window Depending from the settings in the Method Instrument Sending the settings will either be send or not or a window will open which allows to confirm or cancel the method sending Setup Systems and Instruments 67 Fig 87 Cancel Closes the window without accepting the entered settings Send Method All settings of the actual method will be sent to the connected instruments and there become active Report In this dialog the report style can be selected printer set up and the printing initiated The report st
114. nnnossrnnnrnrernsennnne 30 Configuration Knauer UV Detectors with PDA Functions ccccceeeeeee 33 Configuration Knauer PDA Uetechors A 35 Configuration Spectrofluorometric Detector RF 10Axl RF 20A Axs 37 Configuration Conductivity monitor Smartline 2000 40 Configuration Virtual Detector eca e T Eaa 40 Configuration Knauer Intertaces rnein i 40 IPELECION EIERE 46 Configuration Assistant ASM 251 ME 47 Contiquration Ee ul 51 Configuration Thermostats Column COvens 52 Contiguration SwitChing TEE 54 Contiguration E Lee EE 55 Configuration General Settings of Fraction Collectors n nn0annnnonennenenae 55 Configuration Smartline Fraction Collector 2050 57 Configuration MultiValve Fraction Collector 59 Configuration Foxy R1 R2 Fraction Collectors ccccccceecssseeeeeeeeeeeeeeeeeees 60 Configuration other Fraction Collectors cccccssccceeeeeceeeeeeeeeeeeeeeeaasseeeeeess 61 Configuration Completing Instruments cccccceeeesseeeeeceeeeeesaeeeeeeeeeeeeessaeaeeses 62 Conigurationm nS rument TV De EE 63 Contiguration Device Start INDUl EE 63 GOMMGUT ATOR Fins ING WEE 64 Rief CEET 65 Setup Systems and Instruments 0 ccccecccceeseeeeececseccesseeeeeeeessecesseneeeeesescessens 66 VEIT e EE 67 Ate lee 68 Mode Time and Temperature nannnnnannnnennnnnannnoennnerannnrnnrrennnrrnnnrrennr
115. noannneneaaenenaani 3 Switching VAIVES ccccceeseeeeeeeeees 52 103 SEET 85 87 Syringe Volume 71 SYSTEM Getup 64 Thermosiat 89 Time Constant crina 78 79 82 ICH at e 72 TRIO et 44 Tubing Parameters ccccccccecseeeeeeeeeeees 54 TUDING Volume 71 Unconditional collection a0nn00nnoaannnaann 92 User Defined Detector ccccseeceeees 81 UV Detector ACQUISITION c cccecceeceeeeeeeeeseeesaeeeeeees 79 Configuration ccccceeeeeeeeeeeeees 28 31 Eeer eege 79 Eeer 52 Valves FP TOGM AM EE 87 Virtual Detector a asanananannnnnnnnnnnnnn 38 83 FOMU HEEN 85 Aler FE EE 58 60 leben eegen 20 Wavelength table ccccccecsseeeeeeeeeeeees 81 Wiring Manager DOOU 44 Wissenschaftliche Geratebau Dr Ing Herbert Knauer GmbH All rights reserved The information in this document is subject to change without prior notice 2012 10 02 Printed in Germany ClarityChrome are registered trademark of Dr Ing Herbert Knauer GmbH See up to date manuals online www knauer net downloads HPLC SMB Osmometry Wissenschaftliche Geratebau Phone 49 30 809727 0 Dr Ing Herbert Knauer GmbH Telefax 49 30 8015010 Hegauer Weg 38 E Mail info knauer net 14163 Berlin Germany Internet www knauer net Ih KNALIER KNAUER 2012 V1670 5A 0 05 10 12 Koe
116. ns and individual mathematical functions e User settings User selects parameters for peak display and the specification for axes including color from an extensive array of color settings Text labels and lines either as part of the area or anchored to a chromatogram may also be inserted e Useraccounts Sets up access rights and passwords including their parameters e g minimum length validity etc Each user can define his or her own station appearance e Export Optional exportation of all results with or without the chromatogram in various formats ASCII AIA dBase into a file or clipboard e Import Imports chromatograms or mathematical curves which have been saved in text or AIA formats CSW17 CSW32 ASCII AIA from other programs e SST System An integrated module for monitoring the quality of the Suitability Test measurement e LC GC AS controllntegrated add on modules for controlling selected types of chromatographs and autosamplers e Audit trail Records selected events and operations into a special file Records selected operations directly into a chromatogram e Method and calibration history Each chromatogram can easily be displayed under the same conditions as when it was printed exported or saved e Electronic signature Each chromatogram can be electronically signed Signature selection is based on the username or the signa ture certificate Fraction Collection Detector controlled fractionation
117. nts Programming or if included peak report to determine the optimal sampling rate for your chromatography Autozero at start Check this field if you wish the signal to be zeroed at the moment a measurement is started to define the current signal as the baseline 80 Fig Setup Systems and Instruments Acquisition UV Detector Method UV Detector Setup Method Setup Default MODIFIED Select Detector 5 2500 Channel 1 Iw Enabled Detector Method Detector Setup Acquisition Settings Wavelength Table Time Constant 1 0 ei Sec Time Wave min length nm EE 0 00 Sampling Rate 1 wel Hz Options Iw Autozero at Start S Lamp OFF at the End of Run Suitable for Minimum Peak Width at Base ot 0 3330 Min bar Status Description Status Measurement Acquisition Aux Integration Calculation Advanced Cancel Apply Report Help 105 Acquisition UV Detector 2500 setup Method Setup Default MODIFIED Select Detector 52600 Channel 1 Iw Enabled Detector Method Detector Setup Acquisition Settings Wavelength Table Bandwidth E nm Time Constant 1 0 ei Sec Time Wave c it min length nm ee Sampling Rate 17 sei Hz Options Iw Autozero at Start Autozero at WL Change Analog Output On M F Lamp OFF at the End of Run Scale 10V Suitable for Minimum Peak Width at Base 0 3330 Min SES Description Status Ready Cancel Apply Report Help Fig 106 Acquisition UV Detect
118. om the device by clicking on the l button to search for the device It will not be shown in the IP address field if the option Use S N to identify the instrument is enabled If the device receives the IP address from a DHCP server the IP address may change with every start of the device However the identification via S N allows finding the device also if the IP address is different from the address during the 52 Configuration Systems and Instruments configuration The IP address can only be entered manually if the option Use S N to identify the instrument is disabled Use S N to identify the instrument If this option is disabled the IP address of the device can be entered manually This can be either a numeric IP address or the network name of the device We highly recommend using the S N and not the IP address to identify the instrument because devices may automatically receive a new IP address when reset Interface Click the button to get a list of all devices of the selected type connected to the selected Interface The Device Information window Fig 33 allows for the configuration information to be read directly from the connected devices Click on a device and on the Select button to copy the configuration information from the device into the configuration window COM Select from the drop down list the serial port number for the communication port on your PC where the autosampler is connected ID Co
119. on to actualize the list of found devices Click on the desired device and on the button lt Select gt to select a device to configure it The serial number as well as any additional information for the selected device will then be automatically entered into the configuration dialog In this case you must not enter the configuration data manually When complete click Close to exit the dialog and return to the system configuration window Configuration Systems and Instruments 27 Fig 34 Fe Interface only pumps P 2 1L S 1050 S 100 P2 1S P4 1S 10P 20P For pump P2 1L only communication via LAN is supported Some pump models support two types of communication interfaces RS 232 and LAN Local Area Network The settings for these interfaces will be different Intestace AS 232 Intestata la la COM Fart come IP Pret 100m Use SA to dently the instrument w Interface settings Interface RS 232 COM Port Select from the drop down list the serial port number for the communication port on your PC where the pump is connected We highly recommend connecting only one pump per COM port also for those devices that allow connecting more than one device using the serial Y cable Fe If the pump S 1050 should be controlled by RS 232 in the pump s own setup the Interface must be set to RS232 115200 Interface LAN IP Address The IP address can be obtained automatically from the
120. on valves are not supported This is also applicable if the valves are installed in different ASM2 1L housings All the devices will be controlled by only one LAN port a serial control via RS 232 is not implemented 48 Fig 61 Fig 62 Configuration Systems and Instruments ASM 1L Configuration General Left Device Middle Device Right Device Assistant Mame as M2 1L Serial Number Frat 22900001 Interface LAN D IP Port 1 DUU Use GAN to identify the instrument w Leak Sensor Sens Medium Add Info Assistant ASM 2 1L configuration windows tab General If one of the modules should not be controlled it must be removed from the ASM 2 1L configuration There is no option to disable a configured module later on in the Method Setup The configuration window has four tabs one for general settings and one for each of the three device positions left middle and right While the General tab allows for settings for the whole assistant the device tabs give the option to configure the included devices The ASM2 1L will be shown in the System Configuration as a device with up to three subdevices If the ASM will be added to an instrument all configured subdevices will be added General Assistant Name The name of the assistant ASM2 1L appears automatically You can enter any other descriptive name This name will appear on the assistant tab when the method setup or device monitor
121. or is already selected for the next valve an error message The fraction collection option is already selected for another valve will be shown Device Name The default name corresponds to the selected device type All device names must be unique in an instrument configuration Therefore enter different names for the same device types e g if a high pressure gradient is used If the configuration has been read out by using the button the software will name devices from the same type automatically with different names For valves a letter in regard of the valve s position in the assistant will be added e g a valve in left position will be named as ValveL Pumps will get an A or B increment for HPG A or B pump However you can edit the automatically given name as well as the HPG position for the pumps Middle Device Due to that for all three device positions the same devices and settings can be selected the options will only be described for the Left Device tab Right Device Due to that for all three device positions the same devices and settings can be selected the options will only be described for the Left Device tab ae If one of the modules should not be controlled it must be removed from the ASM 2 1L configuration There is no option to disable a configured module later on in the Method Setup Configuration Systems and Instruments 51 Configuration Autosamplers Fig 64 Fig 65 The K
122. or 2600 DAD 2800 identically setup In case of multi channel detectors the Wavelength Table is only valid for the selected channel The setup has to be made for all enabled channels separately The detector specific settings as Acquisition Settings Options and Analog Output On must only be set once and will be the same for all enabled channels Time Constant Select the time constant for the detector from the drop down list The choices are 0 1 0 2 0 5 1 0 2 0 5 0 and 10 0 Sec If a detector model UVD 2 1L S 2520 S 2550 or UVD 2 18 is connected via LAN and only one channel S 2550 is enabled also smaller values corresponding with the higher data rate are possible 0 00 0 01 0 02 und 0 05 Sec Smaller time constants are required for high speed analyses A good rule is to select a time constant which is the reciprocal of the selected sampling rate The relation between the selected sampling rate and its Suitability for minimum peak width is displayed below Sampling rate This is the rate at which data will be sampled by the system For any selected sampling rate the narrowest peak width will be shown for which this sampling rate will be adequate This selection is in Hz data points per second Click on the down arrow to get a list of the frequencies available for the configuration of your system The choices are 1 2 5 and 10 Hz If a detector model UVD 2 1L S 2520 Setup Systems and Instruments 81 Fig 107 or S
123. ort 10001 for Knauer devices Use S N to identify the instrument If this option is disabled the IP address of the device can be entered manually This can be either a numeric IP address or the network name of the device Fe We highly recommend using the S N and not the IP address to identify the instrument because devices may automatically receive a new IP address when reset Interface K 2600 Select from the drop down list the serial port number for the communication port on your PC where the instrument is connected The OK button for leaving this window is only active after defining the serial port number Interface Click the button to get a list of all devices of the selected type connected to the selected Interface The Device Information window Fig 33 allows for the configuration information to be read directly from the connected devices Click on a device and on the Select button to copy the configuration information from the device into the configuration window Configuration Systems and Instruments 35 Fig 48 D Lamp Check this box if the detector is equipped with a D lamp Halogen Lamp Check this box if the detector is equipped with a halogen lamp Flow Cell Enter a description for the flow cell you are using with your UV detector The description will be printed out when you print the instrument configuration Shutter Control S 2600 only This option is activated by default If this op
124. our computer must be restarted in order to complete the installation of ClarityChrom Preparative Do you want to reboot now O1 want to manually reboot later ClarityChrom Installation finishing It is highly recommended to install the latest update from the ClarityChrom CD The update contains the latest driver versions and bug fixes for the Knauer devices To install the update click on Install Updates on the installation menu refer fig 1 This will start the Knauer driver update The installation menu may not be shown after the reboot please run the AutoPlay of your CD DVD drive in the data manager window e g Windows Explorer window or the CD nstaller file from the ClarityChrom CD Knauer HPLC System ClarityChrom Preparative InstallShield Wizard EN Welcome to the InstallShield Wizard for Knauer HPLC System ClarityChrom Preparative The InstallShield Wizard will install Knauer HPLC System ClarityChrom Preparative on your computer To continue click Next i Cancel Installation Update 12 Installation Click on Next to proceed with the installation Knauer HPLC System ClarityChrom InstallShield Wizard Select Components Select the components setup wll install Select the components you want to install and deselect the components you do not want to install Description Knauer AG 3950 Spark Alias Pumps S 1000 Pump S 1050 Pump 5 100 Pump K 1000 Pump K 1001 Pump Knau
125. pRIU Flush Autozero Fig 138 Device Monitor RI detector Device Monitor Knauer UV Detectors The status and as far as occurring the kind of error is displayed in the headline of the instrument field Additional the possibility to execute an autozero and to switch the lamp off or on is given by clicking on the appropriate buttons After entering a new wavelength the Set button becomes active Click on it to send the wavelength settings to the detector Demo Mode 254 nm 0 000m4U D2Lam Wavelength nm 254 Set _Autozero Fig 139 Device Monitor S2500 detector 100 Fig 140 Fig 141 Fig 142 Fig 143 Device Monitor Device Monitor UD 2 1S detector For the button for the detectors UVD 2 1S please refer the Diagnostics section of the chapter Device Monitor Knauer PDA Detectors below Device Monitor Knauer PDA Detectors The status and as far as occurring the kind of error are displayed in the headline of the instrument field The monitor is divided in two sections the monitoring and the direct control area Device Monitor S 2800 The set wavelength and the corresponding actual signals of all configured channels are displayed The actual status of the two digital outs is indicated by the red ON or grey OFF LED symbol Direct Control D Lamp HAL Lamp The On state of the lamp s is represented by a green active LED sym
126. pendent There exist different autosamplers based on the Spark Alias Due to this it is not guaranteed that all options of all models are supported The 84 3 vial tray for the autosampler 3950 is supported in firmware 1 04 or higher A firmware upgrade from former firmware versions is not available The features of the Knauer Optimas autosampler correspond to the AS 3900 Midas beside the built in column oven the Knauer Optimas is not equipped with a column oven The autosampler method window AS includes several different tabs as described below and a tab independent button AS Status AS Status reads out and displays a status summary Hardware Configuration F E Type of Sampler AS 3950 Spark Alias Connection COMTI Tray Cooling Available 55W Not Available Autosampler Status Setup Systems and Instruments 69 Fig 90 Fig 91 Mode Time and Temperature Method Setup Method 1 Select Sampler v 45 3950 Sampler Method Mode Time and Temp J puts and Outputs Mix Methods System Settings Tray Analysis Time min Injection Mode Flush Yolume pl 1 0 Full Loop e Wash Times Preparative Mode Status Tray Temperature 29 C Ready 45 Status as East Measurement Acquisition Integration PDA Method Calculation Advanced Cancel Send method Report Audit Trail Help Autosampler AS 3950 Mode Time and Temperature Method Setup Method 1 Sel
127. pler Mixing Step Add lf Add is selected specify the Amount of a liquid in ul max the syringe volume to be aspirated from a source choices are Sample Reagent A Reagent B and Wash and dispensed to a target choices are Sample and Destination The aspirate and dispense speed depends on the selected syringe and syringe speed System Settings Syringe Speed The vial positions for the reagents and the destination vial you must define first in the Tray setup For reagent A and reagent B one vial position can be selected Make sure that the reagent volume is sufficient for a complete injection series As a destination for each sample one destination vial will be used You can only define the first destination vial 72 Fig 95 Fig 96 Fig 97 Setup Systems and Instruments Mr OD OD JO Oh P Gol Autosampler Mixing Step Mix If Mix is selected enter the number of times to mix 1 9 and the Amount that will be aspirated and dispensed If no destination vial is used the mixing step is performed in the sample vial ef Autosampler Mixing Step Wait lf Wait is selected enter the wait time in minutes If the complete Mix method was executed as the next step the injection will be made The injection method is defined in the method the injection volume will be defined in the sequence System Settings Method Setup Method 1 Select Sampler E 45 3950 Sampler Melbesg Mode Time and Temp Input
128. quipped with a shutter Set Reference Click the button to measure a spectrum to be used as a reference spectrum Spectrum Click the button to measure an absorption or intensity soectrum depending on the signal mode selected for the measurement The measured spectrum will be shown in the spectrum view Autocalibration S2550 The recalibration of the detector will start after clicking the Autocalibration button It is recommended if the flow cell has been replaced Device Monitor Virtual Detector Fig 146 Device Monitor Virtual Detector VD The only opportunity the monitor offers is to start a trigger This will start a run if the system is in Waiting state Device Monitor Assistant ASM 2 1L The status of the ASM 2 1L modules will be shown separately The shown information corresponds with the information shown for the matching single devices Cd 45Mz2 1L Pump Ee mL min fd asm 1L valvem SEE fd GEIB de Fig 147 Device Monitor ASM 2 1L pump AUX valve MultiValve FC Device Monitor Autosampler The shown monitor is only valid for the autosampler S 3950 Spark ALIAS Fig 148 Device Monitor Autosampler AS 3950 104 Fig 149 Fig 150 Fig 151 Device Monitor The status and as far as occurring the kind of error is displayed in the headline of the instrument field The desired tray temperature can be entered end send to the instrument pressing the Set button Addit
129. r Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Knauer Developed by Knauer Beta version Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Developed by Knauer Comment Azura assistant Interface Box Interface Box WellChrom pump WellChrom pump WellChrom pump WellChrom pump Smartline Manager 5000 5050 Preparative pump Azura pump Azura pump Smartline pump Smartline pump Smartline pump Azura UY detector Azura UY det
130. r Setup menu for more information The pumps K 1000 K 1001 S 1000 and K 1800 preparative pump 1800 only are controlled with ClarityChrom if the pump s internal communication in the menu option CONTROL is set to NET baud rate 9600 To control a pump K 501 in the pump s internal setup the menu option CONTROL must be set to Ext RS232 The firmware version must be v 1 23 or higher If you are operating a K 1001 pump fitted with a firmware version lt 5 x you must select the K 1000 for configuration This is due to the status information which the older firmware does not support Otherwise you will get error messages when the pump starts running For those pumps no advanced status information and no pressure trace recording are available Name The name of the selected instrument appears automatically You can enter any other descriptive name for the pump This name will appear on the pump tab when the instrument setup or status windows are displayed Use a name which is unique within the instrument It is not allowed to configure two devices in an instrument with the same name e g 2x S 1000 for a binary HPG system Gradient Mode If the configuration information from the pump have been transferred using the button the correct gradient mode for the pumps S 1050 S 1000 K 1001 and 1800 has been selected as set in the pump Otherwise select the desired gradient mode from the drop down lis
131. r Virtual FC ISCO Foxy R1 ISCO Foxy R2 ISCO Foxy Jr Buechi C660 Key Features e Measuring Simultaneous data acquisition from up to twelve detector channels e Integration There are extensive possibilities for modifying chromato grams The chromatogram can be changed by entering global parameters or interactively through the direct graphical modification of the baseline e Overlay Simultaneously displays a virtually unlimited number of chromatograms and their mathematical modification for example mutual deductions or derivations of any order 6 Key Features e Calibration Internal and external standard calculation methods calibration of groups of peaks and reference peaks method for better identification e Column performance Calculations of peaks in terms of symmetry efficiency resolution all by several methods tangent moments etc e Automated measuring support Sequence tables for any set of samples with or without an autosampler e Post run Automatically displays prints exports and starts other programs after completion of measurement e Batch Automatically batch processes displays exports or prints any number of chromatograms e Summary result tables Displays and prints selected results from all simultaneously displayed chromatograms e User calculations User can define custom calculations in the Result and Summary tables Using the integrated editor you can create your own columns from the original colum
132. r having reached the initial temperature and before the next injection is performed Cooler Temperature Smartline Oven 4050 only Settings from 5 up to 30 are allowed Leakage Sensor Alarm Smartline Oven 4050 only The setting defines the sensitivity of the Leakage Sensor Alarm Values from 1 through 100 are possible With setting 100 the sensor is switched off Setting 1 is related to the highest and setting 99 to the lowest sensitivity of the sensor The Leakage Sensor of the JetStream oven cannot be controlled via software Integration Sets integration parameters When invoked the Method Setup Integration dialog will be opened Fe When using a multi detector configuration each detector will have its own integration table For general description see the Reference Manual chapter 2 5 4 Integration Calculation Allows setting the calculation parameters When invoked the Method Setup Calculation tab will be displayed When utilizing a multi detector chromatogram the tab will be common to all signals For general description see the Reference Manual chapter 2 5 5 Calculation Advanced Additional calculation settings are accessible common for all detectors For general description see the Reference Manual at the end of chapter 2 5 5 Calculation Setup and Programming Fraction Collectors In any system you can include only one fraction collector However the setup window is independent on the configured fraction coll
133. r the measurement since the maximum of intensity may be varied The integration time range is defined by the device parameters Click the id button to determine the optimal integration time You can use this measurement to check quality of the lamp and used optical elements Scan nm Range 194 509 nm This area is used to specify spectral parameters used for your test measurements The range displays the minimal and maximal wavelengths available for the detector The minimal and maximal wavelengths are the limits which are defined by the configuration of the detector If a detector is connected and switched on the physical scan limit of the detector will The Start of Scan is the minimal wavelength with a default value of 200 nm The End of Scan is the upper limit of the scans to be measured with a default value of 500 nm Bandwidth is the wave length range that will be used to measure a data point For the spectra acquisition it is highly recommended to use a bandwidth of 1 nm Mode Select the signal mode for your measurements from the drop down list The choices are Absorption and Intensity Open Close Shutter A click on this button will open close the shutter The label will be changed accordingly Set Filter On Off You can click on this button depending on whether or not you are using an edge filter Device Monitor 103 Dark Signal Click the button to measure the dark current This operation is enabled only for devices e
134. ram window Just as in the window for signal monitoring any section of the chromatogram can be zoomed to by left clicking dragging to select the area and then releasing the gt ae mouse buiton x bi 0 a CH E Ges 1 503 Peak 5 5 lf 3D data or spectra have been acquired with an appropriate detector these data will be shown in a separate window available from the Chromatogram window by clicking Window PDA Window Calibration d CH The creation and use of calibration curves is an extensive part of the ClarityChrom station Calibration curves created from file can contain a virtually unlimited number of curves which then i Calibration standards are saved in separate calibration files Each Pt T ba Da n 3 can be calibrated at up to twenty concentration levels Calibrated results are obtained by creating calibration curves saving them in a calibration file and then subsequently linking a calibration file to a chromatogram and setting the required type of calculation external or internal standard 20 Configuration Systems and Instruments Configuration Systems and Instruments Fig 22 For general description see the Reference Manual chapter 1 2 4 Configuration The Setup and configuration must be performed in some steps First the desired devices must be inserted from the list of available control modules to the left hand side of the configuration screen as shown in the pictu
135. re allowed to overwrite it that the volume matches with the volume of your vials The Configuration Systems and Instruments 61 Fig 78 Fig 79 software uses this volume to calculate the filling level in the vials If any fraction is larger than the set volume the next free collection position will be used for continued collection to prevent that a vial will be overfilled Please refer to the chapter Setup and Programming Fraction Collectors for more details Please make sure that in the Vial Volume corresponds with the real volume of the vials you use Serial Port Select from the drop down list the serial port number for the communication port on your PC where the instrument is connected The LAN interface is currently not supported Serial Port Click the button the try the connection with the device It does not allow getting any configuration information from the device as serial number or rack type Port Settings This button will open a separate menu to select the COM port s data rate Please leave it with 19200 and make sure that the same baud rate is selected in the collectors setup otherwise no communication is possible Port Settings Baud Hate Data Bits Parity Stop Bits Port Settings Please note that the touch pad of the Foxy R1 R2 FC will NOT been BLOCKED if the device will be controlled by software The software cannot recognize inputs via the touch pad Therefore it is highl
136. re below In a second step the selected and configured modules must be moved to the desired instrument into the right hand side of the System configuration window Instrument the Module serial Instrument Instrument type Instrument number module is used in number selector tab selector counter Co nfigured System Configuration modules Setup Control Modul Number of Instyiments 1 Instru ment name oe SE An Instrument 1 lz Instr ent 2 Ei Instrument 3 Instrument 4 3 AS 3950 080079 Instrument Type R Sampler 1 Instrument 1 lic PDA el Name Instrument 1 LC HL Manager 5000 98765 Image for Closed Instrument Image for Opened Instrument Ju 5000 Channel 1 Image for closed Jia 5 5000 Channel 2 SS AA 5 5000 Channel 3 a a instrument AA 55000 Channel 4 Cf HA Smartline 100 BDADOOOS Te E R 5100 AuxPump Instrument 1 Image for opened Ld Smartline 1000 40004 51000 A Instrument 1 instrument S 1000 B Instrument 1 e 5 1000 C Instrument 1 amp Sampler 1 D Spe 5 1000 D Instrument 1 on ic Module GC 5 1000 Smartline 1000 Detect 5 1000 B Smartline 1000 fo S Sen S 51000 C Smartline 1000 Module part AA 52520 Channel 1 i 51000 D Smartline 1000 BB 52600 5 100 Aux Pump Smartline 100 ee D I Detector Instrument method AA 5250 ana ae ae sending setup B Virtual Detector Ext Start Dig Input Sampler 1 fi 2 Di s AA YD Channel 1 Instrument 1 Ready Dig Outp
137. reventing the functionality of the whole network To check the DHCP client setting select Start Control Panel Networking Right mouse click on the Ethernet icon the annotation may differ and then select the menu item Properties Activate in the tab General possibly after scrolling the Internet Protocol TCP IP and click now on the Properties button Compare the opening window with Fig 25 and change the settings as far as necessary Configuration Systems and Instruments 23 Fig 25 Internet Protocol ICP IP Properties General Alternate Configuration You can get IP settings assigned automatically d pour network supports this capability Otherwise you need to ask your network administrator for the appropriate IP settings bain an IP address automaticaly Use the following IP address Obtain DNS server address automatically O Use the following DNS server addresses Properties of the Internet Protocol TCP IP Windows XP Configuration by downloading from the device Fig 26 For nearly all Knauer devices the configuration can be read out and transferred download into the configuration window using the button This proceeding is highly recommended to avoid typos and other problems while a device is configured manually If the desired interface is selected and the device is connected and switched on after a mouse click on the button the device information window will open In this window
138. rrrenrrennne 69 MPSC COUN EE 70 ANG WCU OOS EE 71 EIERE 72 GE Baier elt EE 74 Graden el 74 Standby Seting S E 76 Je EE 76 Ver GI ET EE 17 Acquisition Detector Method EE 78 Acquisition RI Detector Method 79 Acquisition UV Detector Method 80 Channeldepending Settings nuserie a a a a i iN 81 Acquisition S 2900 Monitor Method 82 Channel depending Settings User Defined Detector cccceeeeeeeeeseeeeeees 82 Channel depending Settings Virtual Deiechor 84 ASSISlant ASW TE SEUD ais ex teen raion eae ee ae ee 86 SWICHING Valves SCUD EE 88 Vales re Le EE 88 PDA Delector MENO EE EE 88 PDAS CAN been eeleren 89 PDA PROV ANIC OO sate asset TEEN 89 PDA re Lee DEE 90 Thermostat Column OVENS asnicar coher eee a E Sine 90 ALSO TAU ON WEE 91 Eeer 91 POV GING CO apelin a ee eect ete ences gene ath ne ene 91 Setup and Programming Fraction Collectors cccsceeeeeceeeeeeeeeeeeeeeeeeeeeeaesseeeeeesensaas 91 Fraction Collection Program FC Settings ccccccccccccscsssesseeeeeeeeseseeeeeeeeeeeeeeaas 92 Editing of Fraction Collection Program ccccccccsssssseeeeeeeeeeeeeeeeeeeesessaeeeeeeeeeeeeaas 95 LO VICE MOMOL ceren aa aire 97 Device Monitor PUMP EE 98 Device Monitor Auxiliary Humpe 99 Device Monitor Knauer RI Detectors nnannonnnennnennennnennrrenerrrnrrerererrerrrenrnne 99 Device Monitor Knauer UV Detectors E 99 Device Monitor Knauer PDA Detectors
139. rt the other is an Ethernet port also called LAN Local Area Network Beside the Knauer valve drives for all the devices the desired communication port must be defined in the device Please pay attention to the corresponding notes in this manual and refer to the device s manual for more information how the desired communication port must be configured Please note that for some devices the functionality depends on the selected communication port Connections for device control from computer via LAN Fig 24 gt Some instruments are able to be controlled via Ethernet To perform this create your local area network LAN as illustrated in Fig 24 Connect the LAN ports of the PC and all devices using Ethernet patch cables OPTIONAL to company network only by network administrator EEEE res device mli device Structure of the local area network LAN for an HPLC system Never use the WAN INTERNET port for connection to the PC or any device to be controlled The WAN INTERNET port is exclusively to use for connection to a company network This connection must only be done by the network administrator Make sure that only one DHCP server normally your router is present in your local network All devices to be controlled must be directly connected and operate as DHCP clients Setting Obtain an IP address automatically Any second DHCP server within the same network may lead to inconsistencies in IP addresses p
140. ry Traces Acquisition RI Detector Method Method Setup Default1 Select Detector IV Detector Method Detector Setup Acquisition Settings Time Constant 11 0 ei Sec Sampling Rate 11 wei Hz Options Iw Autozero at Start Suitable for Minimum Peak Width at Base ot 0 3330 Min Det Status Description Status Ready Event Table AS Measurement Acquisition Integration PDA Method Calculation Advanced Cancel Apply Report Help Acquisition RI Detector Setup Time Constant Select the time constant for the detector from the drop down list The choices are 0 1 0 2 0 5 1 0 2 0 5 0 and 10 0 sec Smaller time constants are required for high speed analyses A good rule is to select a time constant which is at least one tenth of the peak width at the base of the narrowest peak of interest The relation between the selected time constant and its suitability for minimum peak width is displayed below Sampling rate This is the rate at which data will be sampled by the system For any selected sampling rate the narrowest peak width will be shown for which this sampling rate will be adequate This selection is in Hz data points per second This is the selection for most chromatography applications Click on the down arrow to get a list of the frequencies available for the configuration of your system The choices are 1 2 5 and 10 Hz It is recommended that you use the peak width s see Graphical Eve
141. s via a drop down list Exp Time min Enter the desired expected time values Mode The single event option is to be selected for switch to collect or waste by time whereas the peak recognition allows collecting peaks by signal level and or slope Every newly detected peak according to the defined thresholds see Parameters option below will be collected in the next empty vial The next empty vial is defined as the next not used vial seen from vial position 1 not from the current collection position The selected mode will influence the display of subsequent settings for that line Event The possible options displayed will depend on the selected mode Setup and Programming Fraction Collectors 93 FE Settings Det Channel Ort Chima 1 lf FC Hese Flaw mL 1 000 Channel 1 sl w EC Heset Pia mL min 1 000 Exp lime Pe ef IT 18 LkcordEvurel Fig 124 Event Definition Single Event Unconditional Signal independent action as selected in the parameter window Peak Recognition Signal Level The collection takes place according to set level thresholds Peak Recognition Signal Slope The collection takes place according to set slope thresholds Peak Recognition Signal Level Slope The collection takes place according to set level and slope thresholds which can be combined in logical AND OR combinations Parameters By activating any field in this column an arrow will appear By clicking
142. s and Outputs Mix Methods System Settings Tray Loop Yolume pl Tubing Yolume pl 100 15 Syringe Volume pl Syringe Speed Needle Height mm 500 v Normal v 5 V Air Segment jw Reset Output Skip Missing Vials jw Headspace Pressure Status Tray Temperature 29 C Ready AS Status as C Gradient Measurement Acquisition Integration PDA Method Calculation Advanced Cancel Send method Report Audit Trail Help Autosampler System Settings Loop Volume wl Select the volume of the installed sample loop in ul If the Preparative Mode is selected in Mode Time and Temp setup the loop volume is fixed at 10000 ul Tubing Volume pl Enter the volume of the installed needle and tubing in ul as labeled on the tube The default settings are 15 ul and for the preparative mode 45 ul Syringe Volume Accessible depending on the configured autosampler Select the volume of the installed syringe from the drop down list The choices are 250 ul and 500 ul The Setup Systems and Instruments 73 Fig 98 250 ul syringe is only available with a special firmware version If the preparative mode is enabled the syringe volume is fixed at 2500 ul Syringe Speed Select the syringe speed from the drop down list The choices are Low Normal and High Needle Height Enter the distance between the needlepoint and the plate holder in mm The default value is 5 mm the distance can be programmed between 2 and 6 m
143. s may not be used for data acquisition Users commonly utilize these so called offline instruments for method development or evaluation of already measured analysis This chapter will mainly focus on the control of Knauer instruments For additional and general information see the Reference Manual chapter 2 Instrument The Instrument window displays with icons a logical sequence of operations and commands necessary for the comprehensive processing of an analysis This Scheme of Data Processing starts from a sample injection taken and finishes with a printout of the results t Instrument 1 Method Method 1 File Monitor Analysis Method Setting View Window Help D d File Name Instrument 1 2011 13_25 51 F yi Sample Sample ID Method Method 1 Mode Single Run 22 5 A bs eels For Help press F1 worki Administrator For Help press F1 worki Administrator ry p File Name Instrument 1 2011 13_25_51 2 Th Sample Sample ID Method Method 1 Mode Single Run Shake Waiting ab ERSE UL 4 a P E R Instrument window To open or display an instrument invoke the command from the Login submenu or click on its icon in the main ClarityChrom window The instrument name is taken from configuration where you can set it The icons from the scheme serve for easy access to all dialogs necessary for the management of an analysis Clicking on the 22 icon will open the device monitor displaying status i
144. subsequently paste the information to another application using the paste command or move the selection to another location in the spreadsheet by selecting the location then using the paste command Copy Use this command to make an exact copy of the selection in the clipboard Once you select Copy you can paste the selection to another application or copy the selection to a location in your spreadsheet Paste This command is used to paste the information currently in the clipboard into the spreadsheet at the location of the cursor Fill Down This enables you to automatically copy spreadsheet information from one field or row down through the rest of the spreadsheet Fill Series This enables you to automatically copy spreadsheet information from one field or row down through the rest of the spreadsheet using an increment that allows changing the setting reasonable Insert Line This command inserts a copy of the line in the spreadsheet below where the cursor is located Delete Line This command deletes the line in the spreadsheet where the cursor is located 76 Fig 101 Setup Systems and Instruments Delete Lines This command deletes the selected lines in the spreadsheet Clear Use this command to clear the information in the selected location You can also use the Delete key from your keyboard for this function Clear All Use this command to clear the information from the entire soreadsheet Select All Use
145. t The gradient type for the pumps S 1050 S 1000 K 1001 and 1800 must match with the gradient mode set in the pump Pump S 1000 Configuration Name S 1000 Gradient Mode LPG sel SerialPort COMI sl LPG HPG A Serial Number 55555 Head LPG Options F Maximum Flow Ausiliary Pump T Maximum Pressure 40 00 MPa Number of Solvents 4 7 Add Info ean Do not stop pump when closing Clarity instrument LPG Cycle Time D sec Cancel Help Cancel Selection options for gradient modes To configure an HPG system correctly you must add the HPG pumps to your instrument in the correct order A B C then D Starting an incorrect 26 Fig 33 Configuration Systems and Instruments configured HPG system an error message will ask you to remove all HPG pumps from your instrument and then re add each pump as described above In case LPG mode is selected it is possible to configure components count and LPG Cycle Time Click the button to open LPG Options dialog Note that the gradient mode also must be set correctly in the setup of the pump it cannot be changed from the chromatography software pumps P2 1L S 1050 S 1000 1800 K 1001 K 1000 Number of Solvents Adjust the count of components to be used in the instrument LPG Cycle Time Set desired LPG cycle time for the configured pump When LPG Options configuration is complete click OK to exit the dialog and return to the pump configura
146. th of 1 nm Signal Mode Select the signal mode for your measurements from the drop down list The choices are Absorption and Intensity For a data acquisition in HPLC usually Absorption will be used Advanced Pressing the Advanced button opens the window for reference correction PDA Advanced Advanced Reference Correction Sample Bw Channel 1 Channel 2 Channel 3 Channel 4 PDA Advanced 90 Setup Systems and Instruments Referring to a wavelength with practically zero absorption for instance the signal noise ratio can be improved or the drift due for instance to a too short heating time of the lamp can be eliminated Reference Correction According to the configuration up to 4 wavelengths can be monitored from the 3D data stream Select the checkbox to activate the associated channel Sample The sample wavelength is defined in the Acquisition tab Bw The bandwidth for the sample channels are also defined in the Acquisition tab Ref Reference is the wavelength at which a reference absorbance is measured as a function of time The reference wavelength compensates for fluctuations caused by changes in baseline absorbance for example due to changes in solvent composition during gradient elution The selected wavelength must be higher than the sample wavelength and be inside of the scan range for this detector Bw Enter the bandwidth of the reference wavelength Limits are 1 to 32 nm in 1 nm steps
147. the IP address from a DHCP server the IP address may change with every start of the device However the identification via S N allows finding the device also if the IP address is different from the address during the Configuration Systems and Instruments 55 configuration The IP address can only be entered manually if the option Use S N to identify the instrument is disabled IP Port The IP Port number used for communication can be entered here Please use only port 10001 for Knauer devices Use S N to identify the instrument If this option is disabled the IP address of the device can be entered manually This can be either a numeric IP address or the network name of the device Fe We highly recommend using the S N and not the IP address to identify the instrument because devices may automatically receive a new IP address when reset Interface Click the l button to get a list of all devices of the selected type connected to the selected Interface The Device Information window Fig 33 allows for the configuration information to be read directly from the connected devices Click ona device and on the Select button to copy the configuration information from the device into the configuration window Valve Type Select from the drop down list the type of the valve The drop down list offers 2 6 12 and 16 position valves Select for each valve the desired type Valve Add Info Click the Add Valve Info bu
148. thod Results SST View Window Help EI fll Lei e ES ent STZEEAEE TEE NEES CEET EE m 2506mutti UY 90 This label and arrow are attached to the chromatogram 2506multi RI d 6 053 glucose alic 4 A 6 577 fructose 5 6300 glycerol 8 12 710 methanol 10 14 833 ethanol 11 A succinic 6 14 min Result Table 2506mutti RI lt ESTD Common for All Signals Amount Amount Peak Compound Response Calibration File Peak T able Calculation g1 Type Name Factor 250 8hr1 STD v Ordnr oxalic 0 240 ZS Ordnr citric 0 0053 Set None View 0 428 6 amp 6 Ordnr tartaric 0 0065 0 581 5 Ordnr glucose 0 0084 Report in Result Table Unidentified peaks 1 037 3 Ordnr malic 0 024 All Peaks eed Dan e Ani 0 612 7 Ordnr fructose 0 0073 C All Identified Peaks e H Ss 0 238 A Ordnr succinic 0 0053 e All Peaks in Calibration DER 0 727 A Ordnr lactic 0 019 Bg Response fact 0 1 125 8 Ordnr glycerol 0 024 10 337 35 378 0 206 2 Ordnr acetic 0 0058 Scale Anfount g l ISTD Amount 12 710 4 079 0 099 6 Ordnr methanol 0 024 Use Scale Factor o 14 833 118 373 11 175 9 Ordnr ethanol 0 094 7 d Total 16 467 Reten Time min 4 561 5 203 44 826 5 423 65 445 6 053 69 369 6 303 44 050 6 577 83 314 8 177 44 854 8 550 37 378 8 900 46 546 Response TS bb bb bb EE bl oa EA KS KS ES CH ES Scale Factor me Units Kg Results A Summary Integration easurement Conditions SST Results or Help press
149. tical software Sections of this manual which refer only to the preparative software are indicated by the marginal icon of the Knauer Prepline system ClarityChrom supports actually the following KNAUER instruments Pumps AZURA Pump P 2 1L isocratic LPG HPG Smartline Pumps 1050 1000 isocratic LPG HPG Preparative Pump 1800 K 1800 isocratic LPG HPG AZURA Pumps P2 1S P4 1S Smartline Pump 100 WellChrom Pumps K 120 K 501 K 1000 K 1001 RI Detectors Smartline RI Detector 2300 Detector 2400 WellChrom K 2300 1 K 2400 1 UV Detectors AZURA UV Detector UVD 2 1L Smartline UV Detector 2500 Smartline UV Detector 2520 Smartline UV Detector 2550 Smartline UV Detector 2600 Smartline PDA Detector 2850 Smartline PDA Detector 2800 AZURA UV Detector UVD 2 15S UV Detector 200 WellChrom Detectors K 2000 1 K 2500 1 K 2600 K 2700 K 2800 Other Detectors Shimadzu spectrofluorometric detector RF 10AxI RF 20A Smartline Detector S 2900 Assistant AZURA Assistant ASM 2 1L Column oven Smartline 4050 JetStream Smartline column thermostat Interfaces A D converter Smartline Manager 5000 5050 interface module IF2 Knauer HPLC Box 96 Autosampler Smartline AS 3800 Spark Marathon AS 3900 Spark MIDAS Smartline AS 3950 Spark ALIAS firmware dependent Knauer Optimas Valve Drives AZURA V 2 1S Smartline WellChrom valves S K 6 S K 12 S K 16 Fraction Collectors Smartline FC 3050 Multi Valve FC Knaue
150. tion lt _ Name S 2520 Interface LAN K Serial Number Cancel IP Port 10001 Inversion Iw Use S N to identify the instrument of Signal Channel Options 1 E Channel 1 A Halogen Lamp Aus Units m4 amp U v Flow Cell Add Info Help S 2520 configuration window Configuration Systems and Instruments 31 Fig 42 Fig 43 Fig 44 Detector UVD 15 Configuration Name uv Aus Unita m l Serial Humber Flaw Cell RE Interface LAN E IP Fort 1 DUU Use GAN to identify the instrument jw Dad lata Cancel Help UVD 2 1S configuration window 2500 Configuration Hame P 2500 Serial Port COMS Seral Number 23456 Y Ass Units rl r Flow Cel 10m Cancel Help S 2500 configuration window K 2501 Configuration Hame K 2501 Serial Fort COMI Seral Humber 34567 Y Ass Units m l Detector Type F 25001 T Flow Cell Add Info Help K 2501 configuration window Name Enter a descriptive name for the detector This name will appear on the detector tab when the instrument setup or status windows are displayed The default detector name will be suggested automatically by selecting the detector type Use a name which is unique within the instrument Serial Number Type in the serial number of the detector you are using Serial Port all detectors beside UVD 2 1L UVD 2 1S S 2520 Select from the
151. tion is chosen the detector will open the shutter only before the run to protect the fiber optics Additionally the dark current will be measured and subtract it from all measured spectra It Is recommended to leave this option always enabled Acquire 3D Data Check the Acquire 3D Data option to utilize the full spectral functionality of this detector If the Acquire 3D Data option is selected you will need additional PDA license option to be able to work with the spectral data If this option is not selected the detector will be used as a multi channel detector The detectors S 2550 and K 2600 cannot perform a permanent scan The scan must be programmed time based Add Info Click the button Add Info to enter more detailed description of the detector When complete click OK to exit the dialog and return to the system configuration window If a serial port as an interface is selected the OK button is only active after defining the serial port number Configuration Knauer PDA Detectors The Knauer PDA Detectors S 2850 S 2800 K 2800 and K 2700 are supported The configuration windows and procedures are identically for all PDA detectors For each instrument only one PDA detector can be added S 2800 Configuration pc OK Name S 2800 Intedlace LAN X 2 SE SerialNumber 61789 i Cancel Charel 2 e j Use S N to identiy the instrumert l 1 240 ren gef N2 410 rem l Halogen Lemp Iw Shutter Control Ee jw
152. tion window Serial Port Select from the drop down list the serial network number for the communication port on your PC where the instrument is connected For error free operation of the binary Knauer Net every device should be connected to an own COM port The implementation of advanced status information generates higher network traffic However connecting two pumps to the same COM port using the y cable may result in communication problems The pumps K 1000 K 1001 S 1000 and K 1800 preparative pump 1800 only are controlled with ClarityChrom if the pump s internal communication in the menu option CONTROL is set to NET baud rate 9600 To control a pump K 501 in the pump s internal setup the menu option CONTROL must be set to Ext RS232 The firmware version must be v 1 23 or higher Click the button to get a list of the devices connected to the selected port The Device Information window allows for the configuration information to be read directly from the connected devices Device Information Device Firmware Version Close Pump 1000 Refresh gt Select Device information dialog The Device Information dialog displays a list of the devices of the given category pumps detectors etc found on the selected interface The IP Address optionally the Device Type the Serial Number and the Software Firmware Version will be shown for each found device Click the Refresh butt
153. tions of the fraction vials have been programmed Please check if the rack selected in the software matches with the rack enabled in the fraction collector The volume for the fraction vials is a default value Please check the real volume of the vials you are using and enter the correct volume Do not fill the vials full to the brim to prevent an overflow Fig 70 Fig 71 Configuration Systems and Instruments Tubing Parameters Ea Volumetric Delay RES O K C Fised Time min Cancel Inner Diameter mm m C Capillary Volume ema S elp Tubing parameters setup These settings enable someone to consider the time delay for transporting the sample from the flow cell to the collector valve If the option Recycling Valve is checked the capillary from the flow cell via the recycling valve to the fractionation valve must be taken into account If you activate the option Fixed time you can enter this time delay directly This value will be valid even if the flow rate is changed Entering the Capillary Volume directly or calculating it by entering the Capillary Length and Inner Diameter the delay time will be adapted to the programmed flow rate of the method The Volumetric Delay calculated by flow rate and capillary volume can only be calculated if the flow rate is fixed over the run time Flow gradients are not supported Solvent Recycling Solvent Recycling Recycling Volumetric Delay e ba
154. tive name for the oven Use a name which is unique within the instrument Serial Number Type in the serial number of the collector you are using Interface The FC 3050 supports two types of communication RS 232 and LAN Local Area Network The settings for these interfaces will be different Interface RG 237 F Gel Interac LAN sl Gel COM Part COM F ddn Obtained using S M a E AA IP Port 1000 w Use S H to identify the irehumert Interface RS 232 Interface LAN Interface RS 232 COM Port Select from the drop down list the serial port number for the communication port on your PC where the instrument is connected Interface LAN IP Address The IP address can be obtained automatically from the device by clicking on the button to search for the device It will not be shown in the IP address field if the option Use S N to identify the instrument is enabled If the device receives the IP address from a DHCP server the IP address may change with every start of the device However the identification via S N allows finding the device also if the IP address is different from the address during the configuration The serial number can only be entered manually if the option Use S N to identify the instrument is disabled IP Port The IP Port number used for communication can be entered here Please use only port 10001 for Knauer devices Use S N to identify the instrument If this option is disabled the IP address
155. ts two types of communication RS 232 and LAN Local Area Network The settings for these interfaces will be different Irkelare R523 Gel Irkelare Lon Gi COM Pot COM Fas T IF Fort hoon if Use S H to identify the irehunment Interface RS 232 Interface LAN Interface RS 232 COM Port Select from the drop down list the serial port number for the communication port on your PC where the instrument is connected Interface LAN IP Address The IP address can be obtained automatically from the device by clicking on the button to search for the device It will not be shown in the IP address field if the option Use S N to identify the instrument is enabled If the device receives the IP address from a DHCP server the IP address may change with every start of the device However the identification via S N allows finding the device also if the IP address is different from the address during the configuration The IP address can only be entered manually if the option Use S N to identify the instrument is disabled IP Port The IP Port number used for communication can be entered here Please use only port 10001 for Knauer devices Use S N to identify the instrument If this option is disabled the IP address of the device can be entered manually This can be either a numeric IP address or the network name of the device We highly recommend using the S N and not the IP address to identify the instrument because dev
156. tton to add the valve specific description or comments for the selected valve When complete click OK to exit the dialog and return to the system configuration window The OK button is active only after defining the serial port number for all valves Configuration Fraction Collectors The configuration procedure is principally the same for all fraction collectors exclusively supported by ClarityChromPrep Smartline FC 3050 MultiValve fraction collector Foxy Jr Foxy R1 and R2 Buechi C 660 and Virtual FC all Developed by Knauer despite the appearance of the configuration windows may differ especially for both Knauer collectors For the configuration of the fraction collectors developed by DataApex e g DataApex FC GP please refer the Clarity main manual Fe In any case only one fraction collector can be configured in one system Fe The fractionation options of the ClarityChromPrep software can only be used by fraction collectors whose drivers were developed by Knauer When configuring a system please be sure to select only a device which was designated as Developed by Knauer in the Module Info column Fe In the configuration of the Knauer Smartline 3050 fraction collector enable the option Use Diverting Valve otherwise the fractions will not be collected Configuration General Settings of Fraction Collectors For fraction collectors only supported racks can be used For those racks the posi
157. ture can be entered and send to the oven by pressing the Set button Set Temperature Control S 4050 only The temperature control can be switched On or Off Digital Outs Events S 4050 only The status of digital outs is indicated The digital outs for S4000 can be controlled as described on page 65 or during a run from the event table see page 67 Leakage Alarm Level S 4050 only The desired alarm level can be entered and send to the oven by pressing the Set button Press the GLP button S 4050 only to display the actual GLP information of the oven Pressing the question mark button a window with instrument information will be shown Device Monitor Knauer Switching Valves Eraus Switching Valves Ve Vave 1 Read d Device monitor for the Knauer Switching Valves The actual position for each valve is displayed in the Position row To switch the valves specify the required positions with the HH buttons and click the Apply button The choices are BLANK and I Inject and L Load for a 2 position valve Device Monitor 105 from 1 to 6 12 16 for a 6 12 or 16 position valve The BLANK space means that the valve will not change its state after pressing the Set Position button If you make a left mouse click on the valve position a menu will open to select the desired position directly If for any reason the communication to a valve is interrupted the corresponding field for the valve position is grayed and no
158. unctionality without a PDA detector select GPC If an instrument type is selected ClarityChrom will check if the selected license options are available If not an error message will be shown that the license options extensions must be purchased AA Instrument 1 Instrument Instrument Type LC PDA z Fig 80 Instrument Type selection Fe The setting is individual for each ClarityChrom Instrument Configuration Device Start Input To start all devices in a run at the same time synchronous start the external start digital input must be selected If no device is selected here you cannot run a sequence Normally the start signal trigger will be sent by the injection device autosampler manual injection valve electrically driven injection valve In the Device section of the configuration window you must select the device which will receive this start signal In the drop down menu Ext Start Dig Input all devices with a Start Input connector will be listed as pumps detectors etc Select the device that is connected to the injection device with a start trigger cable Select a number in the Number column A D converters have often a start trigger input for every channel Knauer A D converters have 4 channels means 4 start inputs Be sure to select the correct input number All other devices have just one start trigger input If is selected the start trigger input will not be active If the configured autosampl
159. ust be set prior to measurement Loi Making changes while measuring would render a measurement void and therefore the parameters are blocked 18 First Analysis Placing the cursor over the chromatograph icon will transform the icon into four icons Clicking on one of the circled icons will display the contents of the current method in the Method Setup dialog eee f Another method file can be selected using File Open Method kya command or the lt 4 icon Analysis Run OL Click the icon to open the Single Analysis dialog and fill in the header of the measured analysis Analysis Sample ID Sample Amount 0 ISTD Amount 0 Dilution 1 Inj Volume mL 0 M Calibration Standard Method Level Comments Control Send method Run Chromatogram File Name Instrument 1 20 04 2011 13_14_11 Moe WR gt ose Enable File Overwrite Counter Data Recovery Cancel Help Fig 20 Single Analysis dialog e Run the analysis using the Run command FAL ai L After running the current measurement time and RUNNING DRAHE inscription will be displayed in the Instrument window in the 12min TASR Status line under the Scheme Analysis Monitoring Click the Ja icon to monitor the analysis procedure again Left click the mouse button and drag to select the area to be magnified and then release the button The original display can be returned by clicking the icon or by double clicking the left mouse
160. ut fs 1000 E g 5 igita input output nea cone number module y Nahe Remove a Module and driver Module Start input Ready output Open help file module version information configuration device device Store Leave configuration without configuration saving System configuration window For this purpose press the Add button and open the list of available control modules The modules are arranged in groups which can be displayed separately For a HPLC system you need for instance the pump out of the LC group and a detector out of the Detector group Each module must be selected and configured separately The figure below only shows all devices from Knauer but all other control modules are also available Configuration Systems and Instruments Available Control Modules Name Vendor Module Info 3 AS AS 3800 AS 3900 AS 3950 Knauer Optimas 3AA Lc ASM2 1L HPLC Box Interface Box IF2 K 1000 K 1001 K 120 K 501 Manager 5000 Prep pump 1800 Pump P2 1L Pump P2 1S P4 1S Smartline 100 Smartline 1000 Smartline 1050 GC 88 Detector Detector UVD2 1L Detector UVD2 15 K 2000 K 2001 K 2300 K 2301 K 2400 K 2401 K 2500 K 2501 K 2600 K 2700 K 2800 5 200 5 2300 5 2400 5 2500 5 2520 5 2550 5 2600 5 2800 5 2850 5 2900 Virtual Detector I MS hu Balance Thermostat S 4000 5 4050 di Valve K 2 K 6 K 12 K 16 2 15 E Hi Fraction Collector Multi alve FC 5 3050 Virtual FC d Knauer Knauer Knauer Knaue
161. y for the RF 10AxI driver beside the maximum data rate RF 20A Axs 5 Hz RF 10AxI 0 5 Hz The Shimadzu spectrofluorometric detector model RF 10AxlI allows a data rate of only 0 5 Hz over RS 232 the model RF 20A Axs up to 5 Hz Fora higher data rate a data acquisition via analog out and an A D converter box e g IF2 is recommended The Shimadzu spectrofluorometric detector models RF 10Axl and RF 20A Axs may not work with COM ports installed with COM port extensions cards or boxes We recommend using a computer s onboard build in COM port Name Enter a descriptive name for the detector This name will appear on the detector tab when the instrument setup or status windows are displayed The default detector name will be suggested automatically by selecting the detector type Use a name which is unique within the instrument Serial Number Type in the serial number of the detector you are using Serial Port Select from the drop down list the serial port number for the communication port on your PC where the instrument is connected The OK button for leaving this window is only active after defining the serial port number Serial Port gt Press the gt button to configure the communication port Port Settings Baud Fate S600 Data Bits Parity Stop Bits Serial Port Settings Select the settings from the pull down menus as shown in figure above The port settings must coincide with the RS 232 parameters o
162. y recommended not to use the touch pad under software control Otherwise the vial position of fractions and other information may not been stored correctly in the Device Monitor Show Rack Configuration other Fraction Collectors FC Buechi C660 Configuration FC Name Cen FC Tubing Parameters Serial Number Solvent Recycling Hack Type Micro Number of viele 240 Vial Volume rl 20 00 T Use RFID Serial Port COM ll Port Settings Device ID 2 id Cancel Add Info Help Configuration window for the Buechi C 660 FC Foxy 200 Foxy Jr and Virtual FC Name Enter a descriptive name for the oven Use a name which is unique within the instrument 62 Configuration Systems and Instruments Serial Number Type in the serial number of the collector you are using Rack Type Select the desired rack type from the drop down list The available choices depend on the individual fraction collector Please make sure that in the configuration the same Rack Type is selected as in the collector s setup Number of Vials The Vials number will be displayed automatically according to the rack type Vial Volume The rack depended value will be displayed automatically However you are allowed to overwrite it that the volume matches with the volume of your vials The software uses this volume to calculate the filling level in the vials If any fraction is larger than the set volume the
163. yle displayed depends on which location the dialog will have been opened from The styles may differ e g between the Calibration window and the Chromatogram window The station is supplied with pre set report styles that are distributed among all windows that contain printing reports commands These styles may be modified copied relocated or replaced with user specified styles The order of the report blocks in the report can be changed if the blocks will be moved with the mouse hold left mouse key on the setup window Report Setup METHOD Page Setup Iw Print IV Info Header _ Lab Header M On New Page Iw Parameters Cancel Report Header Signals Iw Event Table Help Method CC All jw Injection Control Calibration e Active Chromatogram Iw Instrument Control Chromatogram C Only Active Signal Integration Table Open Results Iw Calculation Parameters Save _ Sequence PDA Method Audit amp Signatures PDA Save As Printer Preview Print Print To PDF AREER Hetl El Send PDF Report setup window For general description see the Reference Manual chapter 8 Report Setup Audit Trail Opens the Audit Trail file of this instrument Help Opens the online help file related to the actual window tab Event Table Fe Fig 88 The most left hand tab involves the Event Table For details refer to the Reference Manual chapter 2 5 1 Method Event Table If the lamp of a detector is dis

ClarityChrom Instrument Control 3.0.7 Software Manual

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