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Technical Manual

1. 8 16 15 Counting syringe 8 17 Tos Draining Syringe GSS l te en 8 18 17s MOS Geil ASSY qa 8 19 18 Optical bench lamp amp silent blocks 8 20 19 PCB LED board for 8 21 DO Chamber ASSY 8 22 21 Chamber 5 chambers block 8 23 22 COUDHIDE TIG u u uuu eee eee 8 24 DIAGNOSTICS 2 24 255 26 Di 28 29 30 31 32 33 34 35 30 37 38 39 40 41 42 S DIFF Syringe 8 25 F NIN TIONG OW Rc 8 26 Reagent heating ua eee sts 8 27 Chambers AG 8 28 Sampling percutor motor 8 29 T pe Nolder ASSY m 8 30 Ti ACG M 8 31 CAS De 8 32 UE E E EE A E 8 33 Mixer motor and sensor 8 34 Loading tray lockers assy 8 35 Te E 8 36 Wheels and transfer 8 37 FONT COVER 8 38 I LOR i NNNM RN 8 39 Fleetroni6 DOS 8 40 REC UD THOM EM T 8 41 Internal COMO UCT 8 42 Reagent straw Diam 28mm 8 43 ROC SH ere mere Ps sess 8 44 uoijesiJojne sues anbiunwwod no yinposdas aJja
2. J r RAA022B 4315 9301 vi N S 20 S790 OLUDEOL 3dA1 ONIdd3LS YL00H8Q 76 9 Z 431110 S 5 E s r3 2 c 8 201 ME zW vz0 e e anm A385 ZZ gt lt 5 lt YOLOW JOVINYV 1Y LNOZIXOH NAR o 8 sew gt lt Z gt 8 Zid I ei X U en Q LD CO E WO n LL QO D SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 43 XBA486A LEDs extension JST 1000mm 24 WHITE 24 WHITE 24 WHITE 24 WHITE PHR 4 JST HMR 04V JST CCA018A CCA049A CONTACT AWG 30 24 SPH 002T P0 5S Contacts SMR 001T 0 6 CCA020A CCA050A J38 MASTER BOARD LED BOARD EXTENSION CABLE 10 COLLIERS 2 4x92 DBH001A Creation fer PENTRA 80 ADC 11 10 00 5304 A Description CHRIS Date ECO Rev PENTRA 80 LEDS BOARD EXTENSION BOARD EXTENSION Standard tolerance Roughness 0000 iy napa Coating Coating OSS o N XBAA86A Sheet S This drawing is the property of ABX and may not be reproduced or distributed without authorization DIAGNOSTICS Diag 45 XBA486A LEDs extension JST 1000mm 47 56 PENTRA 80 TECHNICAL MANUAL RAAO22B 3 44 XBA487A LED JST 200mm 24 WHITE 24 WHITE 24 WHITE 24 WHITE J1 LED BOARD EHR 4 JST PHR 4 JST CCA043A CCA018A Contacts SEH 001T P0 6 CONTACT AWG 30 24 SPH 002T P0 5S CCA045A CCA020A LLI co l
3. lup lwo VLGS e4 9 0vSr JBUIBID deo Og NVIN ju JUS viIQ3 eM 980 Sr o1enoeA Og NVIN jwg 9 6 8 o1enoeA giqesiape jou yaqqny deo ym OGt NVN IUp IUS VIQ3 M O09SHL WO 8A0016 YM Jeqqny deo yM NYIN ug YLGS 4 MLSEGO LA 198fou8A owne 8A0016 YM Jeqqny deo UMA NVIN UG IWG WLGS M MLSOSO LA 198lou8A Ouuru9 eese deo uM NYIN YLGS 4 MQOS SO dA 11 199loueA owne Just u VIQ3 EM 099 96 Jguleynoe q uolo g Og NVIN We jwg viIQ3 eM 99 98 J uIe nOpA q uojoeg Og NVIN We jwg VLdGs e4 998 98 J uIe nopA q uojoeg OS NVIW jwg VLds ey 199 98 J uIe nopA q uolo g 8A0016 YM Jeqqny deo uM Og NVIN ug VLGS 4 CSr89 J uIe nopA q uolo g 7 10 TUBE HOLDER ADJUSTMENT des jo suonipuoo Joquunu 40 SI9HONS uNNdeA JOA Al ippV J INPPLJNULW add Sulduald Wed uonisog JapjoY qnl 4 e qer 9 uonisod uonisod g uonisod uonisog eqn jeuondo rSz0189 y uonisod g uonisod g uonisod eqn piepue1S 8L018 uonisod qn epooJeg 99 JOINJOCJNUC NVI e Jop
4. sues anbiunwwod no yinposdas ynad au li Xgy 2421205 e ap ayaisdosd e uejd v 08 VHIN3d S001NW1 x m vo oval noor ayq a G L SATIVA S GINO 3ATVA NOILVNOIS 10 SE LE SATIVA SATIVA SSS VQX NOILVNOISSG YSdWAN LYVd X3GNI uoijesiJojne sues ANDIUNWWOI NO JInpoJdaJ 2142 aU KGW 242108 ej 3jalJd0Jd ej ULJA 08 90011 SNOILYJIHIQOW 30DINH231 310N PUL ASSV JONIHAS SLNADVAY NOLLVNDIS3Q 9 d 39NIHAS LN39V3H VS90VV 3 8 08 09d OIW NOLSId 3SA1 39NIMAS dOL3ONIASSINd3OVdH 9 ZL X EN MANOS a a EE CEE SR J 08 09d NOLSId Ov3M 3ONIMAS VOE0089 H3HSVM HAS IN3OV3H ONHO VZZOVOX z 08 09d AGO d 39018 9V34 ONIHAS Vec0949 Es NOLLVNOIS3Q YSdANNN LeVd X3aNI uol jesijojne sues aNDIuUNWWOI no jJinpoJdaJ nad au Xgy 242008 ajaludoud 1s uejd a 08 VH IN3d 2001 17 SNOILVJISIGOW JNVINHIFL 310N PUL x MOD 10 39Vd Dvig lt O LO PL JLVG 09d ASSY LNSDV3AY SONINAS NOLLVNDISQ 08d ASSY IN3OV3H 39NIHAS SV 6SV 1X NOILVNOIS 0 LYYd XdGNI uonesuojne sues no ynpoidsi eu Kgy 9191908 ejeudoud e 1se ue d aD 08 VU LNAd 9001W1 SNOLLV2IJIQON ANOINHD3L ILON pu wawan NOuv3HO ZR 3D Vd Ovid 20 LO TL 31VQ V O 09d ASNIY NO
5. 08 VYLNdd p JSSEVUX ATEWASSY SAATVA LL SL6LL Baag Z0E9 4LEE WE o 3069 515 8 60 VSZ0QVJ Jnaj2euuoo np a2uaJ8J38 V LON DNO1Hn1312VHlX3 sLOWOL3H HO3123NNO VYSZOQV 8 S3NNVA081J313 5 3g3AI 3 vsa L LEAN MY HN AZ ANNVA _ 290 VQOX 9 ZEN MY AN _ 290 VOX S EEON MY JN AZ INNVA D90EVOX YEN MY AZ INNVA JIDEVOX GEON MY JN AZ INNVA D90EVIX z JA S LHOddnS 3101 v980NVO L Ovdo i3u 55 56 See synoptic diagram on next page Diag 53 XDA755C 5 valves assy LV31 35 4 Pentra 80 synoptic INPUTS OUTPUTS 6 VERTICAL CARRIAGE XBA462A MAIN BOARD XAA456A INPUT OUTPUT INTERFACES 4 M1 g7 XBA396A i Ld n 6 VALVES ASSY B XBAL62A DADIIZA e run DRVER Ra Tee pas on sw2 XBALSBA h a iW XBA393A DEMEURE um uH NJ IO INTERFACE L7 eee BUFFERS 3 Russie sss MATRIX amp COUNTINGS 1 0 INTERFACE D DAD122A 14 I I BUFFERS 1 0 INTERFACE D BUFFERS 1 0 INTERFACE I I BUFFERS 1 0 INTERFACE c BUFFERS 12 VALVES ASSY XDAT51C LENS 128 Kx8 SRAM He 1 LMNE 68HC11 12 4 Legh gt SAMPLE SYRINGE DAD120A DAD107A 14 DAJOOTA XBA399B D _ SSV POWER SUPPLY HARNESS 2 J1
6. 3 25 322 ABAJA Upper aM utu EUN ee 3 26 3 23 XBA396A Infrared 260 3 27 3 24 XBA398B RBC amp WBC electrode coaxial cable 3 28 3 25 XBA399B LMNE flowcell coaxial cable 3 29 3 26 XBA425A Chamber heating 3 30 3 27 XBA427A Emergency position assy 3 31 3 28 XBA431A Infrared photocell 600mm No tab 3 32 DIAGNOSTICS pentra 80 3 29 XBA432A Infrared photocell 600mm 2 tabs 3 33 3 30 XBA433A Infrared photocell 600mm 1 tab 3 34 3 31 XBA458A Switch JST 300mm 3 35 3 32 XBA459A Switch JST 400mm 3 36 3 33 XBA460A Switch Jst 500mm 5 37 3 34 XBA461A Switch Jst Z00mm 3 38 3 35 XBA462A Motor Jst 300mm 3 39 3 36 XBA463A Motor Jst 400mm 3 40 3 37 XBA464A Motor Jst 500mm 3 41 3 38 XBA465A Motor Jst Z00mm 3 42 3 39 XBA469A Horizontal carriage cell extension 3 43 3 40 XBA470A Solenoid JST 700mm 3 44 3 41 XBA471A Solenoid Molex 230mm 3 45 3 42 XBA478A Horizontal carriage motor 3 46
7. lt lt Ot Lc 9 TAGV1 MOVE H3MOILS 9S00XVH Ov 1Z 119V71 MOVE H3MOILS GGOOXVH Oc L 13971 MOVY H3MOILS VS00XVH NV Ny NY Oc L 199771 MOVY H3MOILS G00XVH NOILVNOIS3Gd S38gINAQN L8Vd XAQANI p ntraso Spare part list SA 9 2 PENTRA 80 TECHNICAL MANUAL RAAO22B 1 Spare part list Table 1 Spare part list 2 6 ABCOO6A PCB BACKLIGHT NEC COMPUTER P80 CAE006A SWITCH MICROSWITCH XC5 81 82 CAE010A SWITCH MICROSWITCH XC5 81 CAEO11A SWITCH MICROSWITCH XCH5 S2 CAE019A SWITCH MICROSWITCH XCC5 81 S4 CAEO20A SWITCH MICROSWITCH XGG2A CAYO13AS PCB LCD COLOR SCREEN P80 CBC008A PCB BARCODE INTERNAL P80 CBK043A PCB QWERTY KEYBOARD P80 P60C CBK044A PCB MOUSE P80 P60C CBK045A PCB AZERTY KEYBOARD P80 P60C CBR019A PCB TACTILE BOARD FUTJI PC P80 CBTO13A PCB CD ROM READER P80 CBTO14A PCB FLOPPY DISK READER P80 CCCO09A PCB SDRAM 128MB 100MHZ P80 DACO24A CABLE PRINTER MIC P60 P80 DACO28A CABLE PC MAIN BOARD P80 DADO75A CABLE LIQUID VALVE L 350 10F14 DAD107A CABLE LIQUID VALVE L 480 10F14 DAD109A CABLE LIQUID VALVE L 560 10F14 DAD112A CABLE CARRIAGE BOARD P60C P
8. 2 7 3 Function of valves 2 7 A Try CACC CIRC UI 2 8 SANG k Ort K Tua uuu o Om 2 9 4 2 Basolyse 2 circuit 2 10 ey OSI MOM e ici uk u u S unas sasa 2 11 RSS CEE 2 12 DIRES cu 2 13 4 6 Diluent prime circuit 2 14 2 15 4 8 LMNE transfer circuit 2 16 4 9 LMNE CCUG TCM CUM 2 17 5 Pneumatic diapralm uuu 2 18 DIAGNOSTICS PENTRA 80 TECHNICAL MANUAL RAAO22B 1 Hydropneumatic connections Read this table as follow LV3 2 means connection to port 2 of valve 3 Table 1 Hydropneumatic connection Atmosphere 205 140 DACI LV14_1 1652 70 Count syringe_1 2 05 550 LV17_2 LV17_1 SZ 30 T8 1 Atmosphere 2105 80 LV19 1 LV19 3 1252 500 Diluent reservoir_2 LV19 2 60 e 18 2 1152 230 Drain syringe 2_1 Atmosphere 200 70 V2 2 LV21_1 130 185 Isolator 2 piss es 2 05 300 LV24 2 LV24_ 1 1 52 90 Drain syringe 1_1 oad dica Bore Md go C 3x6 maxi 2000 Diluent input Diluent input 254 190 S 029 2 LV29_1 S 2 04 180 S Diluent reservoir_4 2 05 65 Diluent reservoir_1 Diluent reservoir_1 2 05 370 LV2_1 LVS 205 120 LMNE syringe_2 I OA S 102 50 LV3_1 S 1 52 60 S DVS LV1_1 S 1852 200 o Isolator 1 Isolator 2
9. I I x z CD ROM DRIVE x CX Sg ADDRESS DECODE PMWA iii Dan n ee B PANEL PC CCC005A 650 MB E i 1 N MOTOR BOARD XAAL59A _ CONTROL LOGIC FLOPPY DISK DRIVE p I E oo 8 DATA BUFFERS z 0 te 5 zi 2 HOME RACK LOADING i SS PASSI lt 100 MHz BUS HARD DISK DRIVE 1 44 Mo 3 1 2 D A Si 2 RACK COUNTING 1 SW 12 d 7 E Beee ee ge AES E S RACK COUNTING 2 Eu mi SW 13 pic 16F872 a 4 P170 2 RACK OUTPUT TRAY NEARLY FULL 20MHz SPI 2 TRANSFER COVER OPEN Ld Le 2 TRANSFER COVER OPEN m d poc Hry asam io Rs2s2 29 Rom 2 TRANSFER END CHECK tui HOST INTERNAL 8 pe 2 TRANSFER CHECK 1 a PL Is 2 TRANSFER CHECK 2 lt lt 5 DAD123A I e c e TUBE PRESENCE CHECK LX 661 gt o Uu o IN 2 TUBE HEIGHT POSITION 2 o 2 5 SW_Ut 2 EMERGENCY DOOR STATUS a un UCTRL lt s PIC 16 872 lt TUBE HAND BARCODE READER xl EMERGENCY DOOR SOLENOID 20MHz amp jhh s di HOME RACK TRANFER MOTOR XBA432A 1 4 a eu 6 RACK TRANSFER XBA463A v SPT ere 5V 150mA CCD PS2 QWERTY KEYBOARD i ISOLENOID BOARD XAALT8A CBK043A 8 EAtRACK TRAN
10. Tube Sensor Diag 5 Tube sensor Left Rack Locker Right Rack Locker Push a rack in the rail until it comes under the tracers followers and check that Left Rack Locker and Right Rack Locker status becomes red when tracers are not in a hole of the rack See Left 3 10 PENTRA 80 TECHNICAL MANUAL SENSORS CHECK AND ADJUSTMENT PROCEDURE and right rack lockers page 4 Left Rack Locker Right Rack Locker e Left Rack Locker C Right Rack Locker Diag 6 Left and right rack lockers 1 3 Unloading area Rack End Transfer Push a rack in the rail until it comes in mechanical stop against unloading tray and check that Rack End Transfer status becomes red See Rack end transfer page 4 Rack End Transfer Ejection ray Diag 7 Rack end transfer Unloading Area Full Push the Unloading Area Full switch and check that Unloading Area Full status becomes red See Unloading area full page 4 Unloading Area Full Diag 8 Unloading area full 4 10 PROCEDURE RAS356B 1 4 Covers Front cover Open Right Front Cover and check that Front Cover status becomes red then close it and repeat this operation with Left Front Cover See Front cover page 5 Diag 9 Front cover Thermic Panel Open Thermic Panel and ckeck that Thermic Panel status becomes red See Thermic panel page 5 a 1 EE C Thermic Panel Diag 10
11. zm WIT 92 74 1721728 74 26 GREEN 26 WHITE 26 BLACK GND FD11 21 22 41 GND 2 2 Lu z X lt I gt p lt X O O LL lt O lt F SS cN N N N a FDC22 POSITION CONTROLE ROTATIVE TRAY FDC21 HOME ROTATIVE TRAY FDC41 HOME LOADINC FDC11 HOME PIERCER OPTEK OPB890 T55 95240N1 4 HONEYWELL HOAO0890 T55 B zas 63 03 98 SECONDE SOURCE CELLULE R HONEYWELL A sso e oy9eCr con K Di K D de D d l I DM Date Modification D signation CELLULES IR 2 PATTES CABLEES 170mm MOTEURS CASSETTES VEGA REF du_document XBA342ASS ET Plan dessin le 19 02 96 Par A DA COSTA HEMATOLOGIE Ce plan est la propri t de la soci t ABX et ne peut tre reproduit ou communiqu sans autorisation gt J Diag 21 XBA342A Infrared sensor 23 56 RAAO22B PENTRA 80 TECHNICAL MANUAL 3 20 XBA387A Reagent heating coil UOIJEZIJOYING pajnqiJIsip Jo paxnpo idaJ you pue xgy Jo Ajsadoud ayy si siu SOLLSONSVIG GUVOP 3315 VM supupwuruglQis 91 5S V ZIS MIG S 4990S VL8EVSX 033 NU DUO V 619 66 60 02 30V HL2 3S9 I a2uejsisa buljeau auj Jo auoz 221 S IYJ IAOW UOILL jerJa len SSQUuubDnoW 32U8 13 04 p Jepuejs 09 VHlN3d S4ILJV38H SNILN3dH3S 35V43Jn VH NONO uorjeubisag 02 X 918 ONWHIHL 3N
12. Qquvog d31SVNW 6lf NM TIM OM ALIHM 9ZDMV X t LMS 1 MS m c 3 27 XBA427A Emergency position assy ASSV 9NI333Id 3901 ISON 31 56 Diag 29 XBA427A Emergency position assy PENTRA 80 TECHNICAL MANUAL RAAO22B 3 28 XBA431A Infrared photocell 600mm No tab P29 P30 P37 P41 26 WHITE 26 BLACK 26 GREEN DRAINNING CELL 1 2 LMNE TRANSFER CELL COUNTING CELL PHR 4 JST J29 30 J31 J41 MAS TER BOARD GAL086A PHOTOCELL XBA430A P29 P30 P37 P41 REP DESIGNATION M1 M4 GAINE THERMO ADH 912 7 x 30 Creation for PENTRA 80 CTH ADC 30 06 00 5304 Description CK By Date ECO Rev Designation PHOTOCELL WITH NO TAB JST PLEXI L 600 Standard tolerance ones N XBA431A_ Sheet S This drawing is the property of ABX and may not be reproduced or distributed without authorization Diag 30 XBA431A Infrared photocell 600mm No tab 32 56 SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 29 XBA432A Infrared photocell 600mm 2 tabs 22 15 26 WHITE 26 BLACK 26 GREEN CAX001A PHR 4 JST P80 TRANSLATION MOTOR HOME CELL P80 J22 MOTOR BOARD P80 J15 MOTOR BOARD i _1 Lil V Lil gt Cc gt I lt L v lt c co a P22 P15 CONTACT AWG 30 24 SPH 002T P0 5S CCA018A CCA020A Creation for PENTRA 80 CTH ADC 07 11
13. enn k lt h Diag 1 Bubbling Enter new step value for each bubbling and click lt Accept Values gt To increase bubbling increase the number of steps decrease bubbling decrease the number of steps 100 300 400 FIRST DILUTION LMNE 100 300 400 WBC BASO 100 300 400 RBC PLT 100 300 400 HGB 300 400 500 2 2 p ntra 80 LMNE Flowcell Adjustment Procedure RAS336B DIAGNOSTICS e Concerns Instrument adjustment LMNE Flowcell e Required tools e Allen keys e Flat screw driver e Required products e Latex e Fresh blood sample e Calibrator e Intervention time 1h e Frequency On request e Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 LMNE Flowcell adjustment check This adjustment procedure must be carried out when LMNE flowcell is moved or replaced or when LMNE tube is changed No adjustment is required when LMNE lampe is replaced Enter Menu Service Menu technician Measurment LMNE adjustment allows the adjustment of the entire optical bench parameters A 1 400th dilution of RBC PLT latex is prepared 5 of Latex 1ml of diluent 1 ml of eosinofix in the LMNE chamber This dilution is then transfered toward the optical chamber and injected The measurments are continuousl
14. PENTRA 80 Technical Adjustment Diag 1 Sampler adjustment menu Following adjustments must be checked and adjusted in the following order Make sure to not damage the different switches by moving manually the racks 1 1 Max Rack Loading Max Rack Loading value represents the maximum number of steps run by the Slides to load a rack This motion must be superior to the necessary motion to load a rack but without coming to mechanical stop noise if there is no rack to ensure the correct position of the rack against the Front Plate This motion is never completed when there is a rack because it is stopped by the two switches Make sure there is no rack on the Loading Tray Press Max Rack Loading button See Diag 1 Sampler adjustment menu page 2 When motion is ended put a rack on the two Slides against the Front Plate The gap between the Slides and the Front Plate 1 must be smaller than the rack thickness 2 See Diag 2 Max rack loading gap page 3 2 8 AUTOMATIC SAMPLER RAS347B RACK front plate Diag 2 Max rack loading gap Check the gap then press validate button If the gap 1 is too small mechanical stop noise decrease Max Rack Loading value If the gap 1 is too big increase Max Rack Loading value Repeat the procedure until you get the correct adjustment 1 2 Stop Rack Loading Stop Rack Loading value represent the number of steps after switches dete
15. 24V TP42 E 5V TP40 TOTOY 15V DEA O 15V ees OSV 3 2 High voltage supply check The high voltage test points are located in the right upper part of the Main board and are not adjustable See High voltage test points page 4 Diag 3High voltage test points Table 2 Hight Voltage check ground on TP47 ua 60V Wes RBC aperture voltage 60V WBC aperture voltage 60V TP9 LMNE aperture voltage 4 8 MAIN BOARD ADJUSTMENT RAS331B 4 Main board adjustment Remove the righthand side cover in order to access to the main board 4 1 Hgb blank adjustment Make sure the thermal door is closed Run command Hgb Blank Adjustment from menu Service Technician Measurement Gain The utility carries out a cycle a rinsing cycle of the WBC chamber and continuously displays the voltage used by the converter The adjustement cycle lasts for 20 seconds Adjust to 4 7V 0 02V between Ground and TP1 by the mean of R248 potentiometer See HGB test point page 5 CE Diag 4HGB test point 4 2 RBC PIt gain adjustment A special cycle allows the dilution of the RBC PIt LATEX to 1 220th 10ul of LATEX with 2 2ml of diluent A special count program carries out the calculation of the mean volume in the predefined zones and displays them every 700ms The duration of the measurement cycle is 21 seconds The adjustment are made using R133 potentiometer for RBC and R135 for the Plt
16. Diag 5 Check needle position Diag 6 Needle perfect position Press OK to continue See Diag 7 Check needle position button page 4 PENTRA 80 Check Needle Position Press Enter to Continue Diag 7 Check needle position button At the end of the cycle connect tubing to the inlet when the following screen appears See Diag 8 Check tubing page 5 4 10 PROBE ADJUSTMENT RAS330B PENTRA 80 A Check all Tubing are Connected Press Enter to Continue Diag 8 Check tubing Press OK to continue 2 4 Needle position adjustment Check needle position See 2 3 Needle position check page 3 If probe is too low increase Needle position number of step If probe is too hight decrease Needle position number of step Run the Check Needle Position again to control the good position See above 3 Carriage adjustment 3 1 Carriage home check amp adjustment Remove cover See RAS342 Enter Menu Service Menu Technician Gains MDSS adjustment See 1 MDSS adjustment menu page 2 Press Check Carriage Home button See Diag 9 Check carriage home button page 5 Check Carriage Home Diag 9 Check carriage home button The CARRIAGE position is factory adjusted and must not be changed It represent a A gap of 2mm between the tooth wheel and the front of the carriage See Diag 10 Carriage home position page 5 This carriage positi
17. au X gy 2 200S e ap ayalsdosd ej ueld a 08 VYING LOOTNT SNOILVJISIGOW 300INH 31 310N Pul ove aono ave ES is 41 1 ASSY S3ATVA ZL AINON SATIVA NOLLVNBIS3Q ZL L S3ATVA ZL SATIVA OLSZVOX NOILVN9IS 30 dIaNNN LHYYd X3GNI ac8rvOX 1109 O M SAVA DIT 3ATVA aL8 rvOX 1109 O M ON SAVM C Or1l 3AT1VA uoijesiJojne sues anbiunuiuo no pinposdas jnad au X8V 242005 ej 3JarJdoud ej ysa ued 09 WHINGd Z007W7 SNOILV ISICON 310N PUL m zo ve toon EM lt 61 61 SIAYA Z AINON S3ATVA NOIL VNDIS IG 6L 1 S3A1VA OTr1 3A1VA OCS4NOX m NOILVNOIS3C 38lINDQN LYVd X3ONI gesrvax MOD O M SAVM OTr1l 3AT1VA g8Lgvrvax 1109 O M ON SAVM SATIVA uoljesijojne sues anbiunuiu no jinpo da 2142 In d au Xgy 2 ej ap 3jarJdoJd ej uejd 08 COOINT SNOILV2IJIGON INDINHIIL ILON Pu 0 30V4d OvId ZO LO OL 31VO Z 0Z SINTVA r GINO AATIVA NOILYNDIS 10 Z 0Z p 3ATVA JEGLVAX NE NOILVNOISAG YSdNWAN LYVd X3QNI uoijesiJojne sues anbiunwwo no yinposdas au xgy 242008 ap ayaisdoud ej jsa ued 08 VH LN3d rFO001NW1 SNOIL V2IJIOOI 3QDINH231 310N m vd ZO LO OL 31vQ 08 SIAYA Z AINON JATIA NOIL VNDIS 10 O v2 Z SATIVA NOILVNOISAG LYVd X3GNI
18. 1 1 3 Others 2 4 RAAO22B e Mechanical Hydraulic e Others e Initialisation e Check Motors e Check Valves e Check Sensors e Sampler test e Holder Adjustment e Rack Adjustment e Drain Chambers e Prime Cycles e Unprime Cycles e Clean Cycles e Cycles Counter e Run Park Syringes Position e Run Maintenance Carriage Pos e Calibration Coefficients SECTION 5 1 2 Miniclean 1 3 Concentrated Cleaning 1 4 Autoclean 1 5 Technician Menu 1 5 1 Measurement SOFTWARE VERSIONS e Miniclean e Concentrated Cleaning e Autoclean e Measurement e Gains e Others e Gain Adjustment e LMNE Adjustment e Aperture Current e Pulse Adjustment 3 4 PENTRA 80 TECHNICAL MANUAL RAAO22B 1 5 2 Gains Thermic Adjustment e M D S S Adjustment e Liquid Sensor Adjustment e Vacuum Control e Sampler Adjust e Bubbling 1 5 3 Others e LMNE Calibration e Cycles e Calibration Coefficients e Blank Values e System Tools e Burn In Cycles e Barcode Setup 2 Windows Explorer Access Enter Menu Service Technician Menu Others System Tools then press Launch Windows Explorer button 3 Software releases V1 01 First release of technical manual Second release of the manual e Pentra 80 historic RAH919A technical note V1 01 for P80 with version V1 00 RAH921A technical note V1 01 for P80 with version lt V1 00 RAH938C technical note
19. ASSV SYAENVHO 08 VH LN3d NOLLVNDIS 3Q SNOILVJISIGOW J 10INH231 310N pul 08 09d JagdINVHO ONINIVEQ 9ONIS O V990VVA 08d QV3H ONILNNO9 H38lWNVHO V4SIL O89 EX 08d 09IW 09d SYNLYAdV ONISH O V9SLDED EX Nos SYNLYAdV YSEWNVHO RTE NOILVN9OIS 30 dIANNN 14Vd X3CONI uonesuoijne sues nbiunuuuuoo no yinpoJdes 1ned eu xgv 2191908 ep ayeudoud e 1se ue d 0Z01A1 SNOIL VDIAIQON 3NOINHD3L ILON Pu V 08 09d X90018 SYAENVHO S d38INNVHO NOIL VNBDIS3Q 08 09d 9014 SH38INVHO S HIINVHI 84S9VQX EN MAYOS IOadvasHr olnv 08 09 3 LdO 8H YSLSNOLOHd vesevax 089 099 MOO1d SHAENVHO v YSAENVHO 8209VdX 1 099 08 2149 OSV4 94M H38IWVHO 80L9VOIX EN NOIL VNDIS3Q YFIEWAN 14Vd XANI DESIGNATION INDEX PART NUMBER _ 97 AMA x i SO SA dieu NZ gt D V HSE T W nA A A Ds E X 2 PX VO ce cO A Q lt LL I QO lt Q O Y m lt 157 O RING APERTURE P60 M60 P80 CHAMBER APERTURE 80 GBG156A DESIGNATION J Mey S CHAMBER COUNTING HEAD P80 PENTRA 80 Ce plan est la propri t de la soci t ABX il ne peut tre reproduit ou communiqu sans autorisation MODIFICA TIONS NOTE TECHNIQUE Ind sues anbiunuuio JInposdas au Xgy 343 205 eJ ajals
20. CCA043A 6 EHR 4 CCA044A FHR 6 Ref JST EAR 4 7 COLLIERS 2 4x92 CCA045A DBH001A Contacts SEH DOTT POJE Ref JST EHR 6 CLAOASA Contacts SEH 001T P Creation CT DB 106 10 00 15304 A Modification VisiDes Date DM Designation TORON MOTEUR lg 700 JST Tolerance Generale tat de Surfs Mati re Traitement Rev tement DIAGNOSTICS N XBA465A Folio Z Format A4 ECh Dim en millim tres Ce plan est la propri t de la societe ABX il ne peut tre reproduit ou communiqu sans autorisation Diag 40 XBA465A Motor Jst 700mm 42 56 SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 39 XBA469A Horizontal carriage cell extension 26 WHITE ERR ND 26 GREEN A NO 26 RED LE ILED J11 MOTOR BOARD PHR 4 JST 5102 4 MOLEX HORIZONTAL CARRIAGE CELL p P11 EER i CCA018A CAK005A CONTACT AWG 30 24 SPH 002T P0 5S CCA020A Description Chk By Date ECO Rev PENIRA 80 HORIZ CARRIAGE CELL EXTENS Standard tolerance Roughness Material Treatment Coating Coating S o O N XBA469A Sheet S This drawing is the property of ABX and may not be reproduced or distributed without authorization DIAGNOSTICS Diag 41 XBA469A Horizontal carriage cell extension 43 56 PENTRA 80 TECHNICAL MANUAL RAAO22B 44 56 3 40
21. INTERLEAVED 2 OF 5 no check digit control 1224488 reading12244881 121314151617 reading 121314151617 CODE 39 no check digit control reading 12345ABCDE reading 12345ABCDEW without C D CODE 128 control without check digit transmission e 123456abcd QWERTY 12345ABCDE azerty CODABAR no egality control between start stop character 123456 37859 reading 37859 reading 123456 Diag 1 Barcode reading test 2 4 EXTERNAL BARCODE CONFIGURATION RAS344B 2 Barcode reader setup An audible beep occurs after each reading Read all the labels from top to bottom and from left to right When the last label is read check once more on the test labels For the code 12 of 5 to avoid a bad interpretation it is mandatory to hold the reader in order to read the entire code If the reading test has failed for the DATALOGIC reader proceed to the barcode setup as follows Restore system default configuration Enter configuration environment CP507 WEDGE for IBM AT ALT mode AZO Disable all family code AB11AB 0116 Code 39 no C D 1 to 16 char AC1104
22. It is mandatory to perform the Probe adjustment procedure after this adjustment 1 Alignment check and adjustment This adjustment procedure must be carried out when chamber assembly is moved or replaced Switch off the instrument and disconnect power supply cable Remove cover See RAS342 Move the carriage over the rinse chamber then gently push down on the probe until it touch the top of the rinse bath See Diag 1 Rinse chamber position page 2 Diag 1 Rinse chamber position Without lifting the probe gently push the carriage over the WBC BASO chamber See Diag 2 WBC chamber position page 3 2 4 CHAMBERS ADJUSTMENT RAS329B Diag 2 WBC chamber position Check that there is the same height between probe and Rinse and WBC Baso tops of chambers If you need to get a better position free the chamber support by loosening the 5 screws See Diag 3 Chamber support screws page 3 Diag 3Chamber support screws Locate probe See Diag 1 Rinse chamber position page 2 Tighten gently the left upper screw Without lifting the probe gently push the carriage over the WBC BASO chamber See Diag 2 WBC chamber position page 3 Check that there is the same height between probe and Rinse and WBC Baso tops of chambers If necessary adjust position by moving up and down chamber support Perform an alignment check as describe at the beginning of this procedure When adjustment is correct tighten sc
23. PDW Platelet Distribution Width is calculated from the Plt histogram The PDW is represented by the width of the curve between 15 of the number of platelets starting from 2 fl S1 and 15 of the number of platelets beginning with the variable top threshold S2 gt Diag 7 PDW calculation 2 3 WBC and differential count 2 3 1 General principles The WBC count is carried out twice by two different sensors e In the BASO count chamber at the same time as the BASOS count e In the optical chamber during the acquisition of the LMNE matrix The reference count is the one obtained in the WBC and BASO count chamber 7 12 PENTRA 80 TECHNICAL MANUAL RAAO22B 8 12 2 3 2 BASO WBC Count Detection principle is the same as for RBC Differentiation betwen BASOs and other leukocy tes is obtained by means of the BASOLYSE Il specific lysing action All the WBCs are counted between the electrical threshold 0 threshold lt BA3 gt The basophils are located from threshold lt BA2 gt to threshold lt BAS gt Diag 8 WBC BASO histogram Table 3 WBC Baso count Technical characteristics of the WBC BASO counts Initial blood volume 10 ul CBC or CBC DIFF Method Impedance Vol ABX BASOLYSE II 2000 ul Aperture diameter 80 um Final dilution rate 1 200 Count vacuum 200 mb Temperature of reaction 35 C Count period 2 X 6 seconds Results WBC Number of cells per volume x coefficient of calibration B
24. The liquid sensor test points are located at the bottom of the Main board See Liquid sensor test points page 6 Jia iC AEF B C Draining 2 6 5 Dee des 5 x T 81 189 B rae Ec w ia LMNE Transfer TP67 R77 R9 E al Es B atl p me J15 e re E pul uaz h ey Ta male I FP aes EE FX1 FX11 Diag 6Liquid sensor test points From menu Service Technician Measurement Gain Liquid sensor adjustment adjust all the different voltage values according to the following table Table 3 Liquid sensors adjustment Liquid Sensor Test Point Potentiometer Range N Connector Draining 1 TS R151 4 5V x0 3V X J80 Draining 2 TP48 R78 4 5V x0 3V X J29 Counting5 TP68 ZEE Transfert LMNE R177 ALIS Ver 6 8 MAIN BOARD ADJUSTMENT RAS331B 4 5 Motor current adjustment The motor current test points are located in the middle of the Main board on the left See Motor test points page 7 Sampling syringe ve TP66 R114 mj TPES Eu AS Em H El HE Diag Motor test points Check and adjust if necessary the motor current according to the following table Table 4 Motor current oampling syringe TP66 R114 2V 0 05V Carriage IPOS R126 15 4 6 Threshold adjustment The t
25. Verti carriage motor current J MOTORS RS232 1 AU 0 M LEDS CPU CPLD CPU CPLD BOARD E uss JTAG EMERGENCY i POSIT ASSY I i VALVES PIC 16 cc DRAINING 1 VALVES 5 vaty H mew m DETECT LI LJ Sensor Contro _ gr E ov DRAINING 2 VALVES ro GND f DETECT 24 30 ll a IS COUNTING DETECT VALVES 31 35 LT i o TRANSFERT DETECT VALVES ae 85232 2 P C PANEL TH E es gr a gt H gt 2 2 2 z g Power SUPPLY 5232 3 gt S Seo 5 P eae P m z t 216 m 2 Alimentation du lecteur codes barres sur JS INTERNAL BARCODE Smo m Power supply of barcode reader on J5 rm a m z s 15 gi E 2 m za ds ifs ir VCC on J5 8 15 8 not connected m E Diag 1Main board general view 3 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE 3 Main board check 3 1 Power supply check The power supply test points are located at the bottom of the Main board and are not adjustable See Power supply test points page 4 un _ wid J13 m FA E TPBH Dag id H s D s L Bez Ben EN ED 3 ae uaz a Diag 2Power supply test points m z 745 FX18 Table 1 Power supply voltages check Ground on TP47
26. 91025 EY 9215 MJA S 199uS 800 8 YS 2120 8 MU buijeo5 V Wel 10 57 60 IOV HLI Uolj29UU03 Mau bumoto 0100 0041 Se LOS 0L60 00Ld 1 uotjeubisep oy AOE 2 09 279483 SEBZ SL TLL eus 111 euius 450H XL 50 QXH HOSN3S LNS34 LOS 0160 0041 43Q0v1H 3002 4v8 0010122590 0S xeu F MZ SF AS Id LOS 0160 00141 WIS n CBCOO8A Barcode reader Diag 3 5 56 PENTRA 80 TECHNICAL MANUAL RAAO22B 3 2 DADO75AS Flat cable 350mm 14pts VERSION SIANDARD LC220 J35 J36 MASTER BOARD C Ta lt O m LL N lt gt cO i 2 Y O gt MICROS 5 6 VALVES BOARD LC220 4 6 VALVES BOARD HE10 F14 HE10 F14 HE10 F14 HE10 F14 SURMOULE SURMOULE NOTA Se r f rer la proc dure de fabrication des limandes RCFO13A C sse 14 04 95 Ralionger lo limande de 30 mm pour une utlisotion commune sur MIGROS et LC220 B Compotibiite entre version standard et version surmoul e A jooyeqo n SSCS DM Date Modification D signation LIMANDE 5 6 ELECTROVANN MICROS DADO75A 4 6 ELECTROVANNES LC220 REF du document DADO75AS Ns RE EE HEMATOLOGIE Ce plan est la propri t de la soci t ABX et ne peut tre reproduit ou communiqu sans autorisation O 5 gt Diag 4 DADO75AS Flat cable 350mm 14pts 6 56 SECTION 3
27. DIAGNOSTICS e Concerns Optical bench e Dismantling e Replacement e Control e Required tools e Hexagonal keys e Dynamometric screwdriver A302 A301 A300 e Flat screwdriver e Torx keys e Required products None e Intervention time 30min e Frequency On request e Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 Optical bench dismantling Switch the instrument off Disconnect power supply cable Remove the lefthand side vertical panel and the top cover to access to the optical bench From the Optical bench disconnect the following cables See Diag 1 Optical bench dismantling Ground Ground fitting Signal cable page 2 1 i E M b gt K a in Net Re Diag 1 Optical bench dismantling Ground Ground fitting Signal cable Disconnect tubes from LMNE syringe e Inlet 1 e Inlet 2 Disconnect tubes from valves e 1 Inlet 1 e 4 Inlet 2 Diag 2LMNE syringe and Valves disconnection Disconnect the Optical bench lamp power supply connector See Diag 3 Optical bench lamp disconnection page 3 2 6 OPTICAL BENCH DISMANTLING amp REPLACEMENT RAS341B Diag 3Optical bench lamp disconnection Remove the Optical bench electronic board unscrew the 2 screws maintaining the boa
28. FRONT PANEL amp COVERS DISMANTLING RAS342B Remove the Motor board protection 1 by loosening the 2 CHC M4X6 screws See Diag 11 Motor otection 1 page 7 hoard nr DOara prc LIC Diag 11 Motor board protection 1 Remove the Motor board protection 2 by loosening the CHC M4X6 screws See Diag 12 Motor board or tion 2 page 7 Diag 12 Motor board protection 2 7 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE Remove Ejection Tray by unscrewing 2 CHC M4X6 and 2 FHC M4X6 See Diag 13 Ejection tray page 8 Diag 13 Ejection tray Remove Upper Cover by unscrewing 6 CHC M4X6 screws 2 on the right side 2 on the left side and 3 at the rear of the instrument See Diag 14 Upper cover page 8 Diag 14 Upper cover 8 8 p ntra 80 Internal barcode adjustment Procedure RAS343B DIAGNOSTICS e Concerns e Adjustment of the internal barcode reader e Barcode configuration e Required tools e Allen keys e Flat screwdriver e Required products None e Intervention time 15 min Frequency On request e Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 2 6 1 Barcode reader adjustment check Remove cover see RAS342 procedure Load a rack with 2 tubes fitted with the same large barco
29. MO WGO C 6G9S JOUIRIOIIN uo1oeg 9 uonisoq Jopjou eqn 9 e qe ueuusn pe eqoud anba Joyeydepe ue poy deo eui Aq paljddns jou Jexons e S1d999e qn1 su pMe DBoJOIAj deo INOUJIAA wO MO LUG O 916995 JOUIeLOIOII uo1oeg SUOI IPUO7 Jaquinu SUOIJEAIASGQ des Jo d SI9H0NS uinnoeA SAHIPPY el pow Jounjoeynueyy qnl qel PENTRA 80 TECHNICAL MANUAL PROCEDURE 8 Compatible tube list for rack Manufacturer Model Additive Vaccum ane Type of cap number conditions Becton D Vacutainer 367651 K3 EDTA 5 2m With cap Hemogard Becton D Vacutainer 367652 K3 EDTA 5m 3ml With cap Hemogard Becton D Vacutainer 367654 KS8 EDIA 5m 4 5ml With cap Hemogard Becton D Vacutainer 368452 KS EDIA 5ml With cap Hemogard Started 04 1901 Terumo Venoject VI O50STK KS3 EDTA 5ml 5ml With cap Rubber with groove Terumo Venoject VI O53STK K3 EDTA 5ml 3ml With cap Rubber with groove Greiner Vacuette 454036 5m 4ml With cap Hemogard Greiner Vacuette 454223 K3 EDTA 5 4 5m With cap Hemogard 10 10 p ntra 80 Power supply replacement Procedure RAS339B DIAGNOSTICS e Concerns Instrument dismantling Power supply e Required tools e Hexagonal keys e Flat screw driver e Required products None e Intervention time 30 min Frequency On request
30. The Pentra 80 as been designed to produce less than the required level of electromagnetic interferences in order to operate in conformity with its destination The electromagnetic interferences caused by the Pentra 80 are limited to a level allowing the correct operation of other instruments in conformity with their destination In case of problems check that the instrument is not placed in proximity of electromagnetic fields or short wave emissions radars X rays scanner etc 2 6 Environment protection Used accessories and consumables must be collected by a laboratory specialized in elimination and recycling of this kind of material according to the legislation 3 4 PENTRA 80 TECHNICAL MANUAL RAAO22BA 4 4 p iitra Specifications 1 Technical specifications 1 2 UN DN ciii 1 2 1 2 Throughput Analyses 1 2 1 3 Tube identification 1 2 GUNS E E 1 3 CIE ES Ies T 1 3 1 6 Measurements 1 3 2 Physical specifications 1 3 2 l Power reguirementS LLL o 1 3 2 2 Operating temperature and humidity 1 3 2 3 Dimension and WEIS cute peterent isai 1 3 2 4 Minimum specimen volume ss 1 4 2 5 OUR
31. button See Diag 12 Check carriage position button page 7 Check Carriage Position The sampling needle moves over a chamber Check the probe position as describe above See Diag 6 Needle perfect position page 4 Press OK at the following screen See Diag 13 Check needle position button page 7 to move to the next chamber and continue until the end of the cycle Diag 12 Check carriage position button Check Needle Position Press Enter to Continue Diag 13 Check needle position button At the end of the cycle connect tubing to the inlet when the following screen appears See Diag 14 Check tubing page 7 A Check all Tubing are Connected Press Enter to Continue Diag 14 Check tubing Press OK to continue 3 3 Carriage Positions adjustment Needle Up and Down position is the same for all the chambers and cannot be modified from one to another adjustment by Needle Position value Before any adjustment check the probe position in each chamber with the Menu Service Menu Technician Gains MDSS adjustment Check Carriage Position option Adjust probe position for all the chambers separately in the software cycle order See Diag 15 Carriage positions values page 8 7 10 PENTRA 80 TECHNICAL MANUAL PROCEDURE DIL 1 Carriage Position 1779 WBC BASO Carriage Position 1059 LMNE Carriage Position 737 RBC Carriage Position 1053 Check Carriage Position
32. page 11 and plunge the aperture in distilled water Diag 19Counting heads dismantling Replace EPO joints by new ones See Diag 20 Counting heads aperture joint page 11 Diag 20Counting heads aperture joint Clean the chamber and the counting head with liquid soap Do not introduce any sharp instrument inside so as to avoid damaging the inside of the chamber and the aperture Do not manipulate the aperture using hard instruments Clean the aperture with a piece of soft paper or preferably in between 2 fingers Rinse thoroughly with distilled water Dry the exterior of the chamber with a soft paper It is recommended to reconnect the tubes on the counting head before reassembling the electrode and the chamber in order to avoid applying constraint on the chamber Do not apply too much pressure on the electrode fixation screws as it can break the aperture Tightening torque 400 m Nm 17 Ozf in Heassemble the electrode Repeat the same operation with the other counting head 11 12 PENTRA 80 TECHNICAL MANUAL PROCEDURE 12 Tape on trays If the Sliding bands located on Loading and Ejection trays are damaged replace them using the Tape JAJOO7A included in the kit See Diag 21 Sliding bands page 12 Use Acetone to clean the trays 9 E u 174 PA Loading tray Ejection tray Diag 21 Sliding bands 13 Check up after intervention Before reassembling the covers start the i
33. 2 06 140 ZEE V es di i 1 52 300 LL ii renee um 4 T 2 HILL ie ns 7 t 2 72 202 7 DRAN pe 3 10 SYRINGE ES 1 21 152160 152160 152180 z 2 x x N To eo 2 L 2 e a q gt lt a 152 130 III r gt Hr 21 LILI e lt ae L ET NOTCHED PG r TUBING 55 ll e es lt 1 x EWE d 1 mm P ae C2 21 C2 22 Se 1 GLA DIC CI LMNE SYRINGE REAGENT SYRINGE Ti 2 5 190 mr 5 s M4 2 06 20 G 130 320 IPAE 13020 152 230 L We J y 2 06 550 5L LL T l l FILTER l I l l l l Io Ia I iS lun IN la lun Lt un Ro UT gt Ro a UT ES See en an IF espn ee C 3x6 1 2000 MAXI r 1 WASTE ABX DILUENT LV1 Differential diluent Selects flow cell sheath 2 or DIFF bath LV3 Differential diluent Selects flow cell sheath 1 or sheath 2 LV4 Flow cell sample supply Opens pathway from the DIFF bath the flow cell L 4 LU LV5 Flow cell sample injector Opens waste path for sample LMNE syringe LV6 Rinse output control Sele
34. DIAGNOSTICS e Concerns e Workstation setting save and restore e Analyzer setting save and restore e Analyzer settings printing e Required tools Floppy disk e Required products None e Intervention time 30 min e Frequency On request e Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE For analyser the followings data are saved Cycles counter Reference CTN values Temperature recorded Calibration coefficient Motor Home and Motion Values Needle Tube Holder Position Window Counting and Reagent Levels For workstation the followings data are saved Types alarms level Global Variables limits level pathological levels and the version number 1 Analyser setting Enter Menu Settings Save restore Configuration Diag 1 Save restore settings screen 1 1 Analyser setting save On Hard Disk Click on Save Setting On Hard Disk button to save analyser setting on hard disk See Diag 1 Save restore settings screen page 2 Click on Validate button on following screen See Diag 2 Validate page 2 PENTRA 80 A Diag 2Validate When the following screen occurs See Diag 3 setup saved page 2 click on validate button PENTRA 80 A Diag 3setup saved On Floppy Disk Insert a f
35. L1 L SHAPER ACQUISIT CONTROL SHAPER DAD075A 14 CHAMBER HEATING LOGIC 128 Kx8 SRAM RBC 68HC11 12 4 Logl gt XBA398B FAN DAK017A DAD109A 14 XBA398B WBC BASO I L ae 3MHz 12 4 FLASH EPROM 1Mo x 8 512 Ko x 16 HIGHT SRAM 512 Ko x 8 LOW SRAM 512 Ko x 8 E U XBA389A E MM ERRORS gt REAL TIME CLOCK RAM 32 Ko x 8 N XBA461A 27 XDA605A LMNE TRANSFER b TP67 R177 1 0 INTERFACE D BUFFERS LEDS BOARD XAA429A J START CYCLE LEDS 4 XBAL86A J p380 XBAA87A y Q Q RED GREEN XBA488A 2 JP XBA322B COUNTING DETECT XBA431A Jd TP68 R171 Cy DRAINING 2 DETECT XBA431A 4 R78 T DRAINING 1 DETECT XBA431A TP52 R151 TUBE BARCODE READER CBC008A eo XBA425A b 4 RS232 REAGENT HEATING COIL SENSOR PIC 16F872 INTERFACE z Mgs BAR 5V 630 nm 500Hz We qp S XBA387A EXTERNAL REAGENT WASTE DILUENT LEVEL DETECT FIF0 9 3 n 12 1 TFT COLOR DISPLAY XBA427A SW2 NEEDLE HIGH MOTOR RESISTIVE TOUCH SCREEN CPU 32 BACK GROUND TUBE PERCING MOTOR POWER 68331 25 MHz SW1 NEEDLE LOW o DRIVER a SW3 6 TUBE RECOGNITION INI2 IN 7 JTAG INTERFACE d amp
36. May cause a low incorrect RBC count Blood samples containing the agglutinated red blood cells may be suspected by elevated MCH and MCHC values and shown by examination of the stained blood film e Cold agglutinins IgM immunoglobulins which are high in cold agglutinin disease may cause lower RBC and Plt counts and increase MCV Hgb Hemoglobin e Turbidity of the blood sample Any number of physiological and or therapeutic factors may produce high incorrect Hgb results To obtain accurate hemoglobin results when increased turbidity of the blood sample occurs determine the cause of the turbidity and follow the appropriate method below e High WBC An extremely high WBC will cause excessive light scatter In these cases use reference manual methods The diluted sample should be centrifuged and the supernatant fluid measured with a spectrophotometer e High lipid concentration A high concentration of lipids in the blood sample will give the plasma a milky appearance This condition can occur with hyperlipidemia hyperproteinemia as in gammapathies and hyperbilirubinemia Accurate hemoglobin determinations can be achieved by using reference manual methods and a plasma blank e Increased turbidity may also be seen in cases where the red blood cells are resistant to lysing This condition will cause an incorrect high Hgb result but may be detected by observing the abnormal MCH MCHC values and the increased baseline on the leading edg
37. On the motor board disconnect the following connectors e J19 ustep motor e J22 Tube mixer motor home e J23 grabber status e J21 head locking solenoid 1 5 Mixer assembly replacement If the Pentra 80 serial number is lower than P800154 locate the Mixer assembly location mark the contour of the mixer with a pen See Diag 5 Mixer location page 3 Diag 5Mixer location If the Pentra 80 serial number is higher than P800154 2 squares are fixed on the support plate to keep the location Never unscrew the squares they are factory adjust and that is the only way to keep the mixer assembly position Unscrew the 3 CHC screws then carefully remove the Mixer assembly Do not remove the wedge A The thickness of this wedge is factory calculated and associated with this instrument to keep the correct distance between the mixer and the rack rails apart from the mixer assembly Note down the parameters writted on the new mixer Install the new mixer assembly by following the previous steps backward 3 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 2 Adjustments If the Pentra 80 serial number is higher than P800154 no adjustments are necessary except entering the new movement values as follow e Plug the power supply cable then turn on the instrument e Enter Menu Service Technician Menu Gains Sampler adjustment See Diag 6 Technical adjustment screen page 4 e In the Mixer Mechanism A
38. Run command RBC PIt Gain from menu Service Technician Measurement Gain Target values e JJ 00 FRI FX3 0 FF Il N 2 O avr A q R ze 4 Oz FE Ri Jor e gil ewe se x e ts m cae TPS p P 9 9 Ec FE ee ee gem 2S cor a rra E R onus J wg mes aSa m aa e 8 8 ES WBC Baso GAIN ex m T2 D arse ren an us TP39 s P ae s TP15 3 M mo MEIN eS e e ro EFI E EE jm B ec LE mer emm e NEN TPS Ter ER al d TF44 cres GA amm n cea SE umm 7 Diag 5 Gains RBC PIt LATEX thoroughly before sampling 5 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE 4 3 WBC Baso gain adjustment A special cycle allows the dilution of the RBC PIt LATEX to 1 733rd yl of LATEX with 2 2ml of diluent A special count program carries out the calculation of the mean volume in the predefined zones and displays them every 700ms The duration of the measurement cycle is 21 seconds The adjustment is made using R134 potentiometer See Gains page 5 Run command WBC Baso Gain menu Service Technician Measurement Gain Target values WBC Baso 102 Mix RBC PIt LATEX thoroughly before sampling 4 4 Liquid sensors adjustment
39. See RAS348 Unscrew the 6 CHC M4x6 screws to remove Internal PC from the cover Unscrew the 4 CHC M3x6 and the 4 CHC 4 6 screws See Cover plate fan page 2 Do not remove Left Angle Plate A in order to keep the PC Cover adjustment y Diag 1 Cover plate fan Disconnect fan and remove cover plate Unscrew the 4 CHC M3x6 screws M3 Washers and lift Hard disk CD ROM Drive Floppy Reader assy See Hd assy screws page 2 Diag 2 Hd assy screws 2 6 PROCEDURE RAS352B Disconnect flat cable from Hard Disk then from CD ROM Drive See Flat cable disconnection page 3 j Z MON uen LES graba que teen Fu ee jee hee Diag 3 Flat cable disconnection Disconnect FPC cable from Floppy disk Reader See Floppy disk reader connection page 3 pull gently brown part of connector up 1 Pull up FPC flat cable 2 Blue band of FPC cable in this position Diag 4 Floppy disk reader connection Remove Drives Support with Hard Disk Floppy Disk Reader and CD ROM Reader 3 6 PENTRA 80 TECHNICAL MANUAL PC CD ROM DRIVE REPLACEMENT PROCEDURE Unscrew the 4 CHC M3x4 screws and remove Drives Support with Hard Disk See Drives support page 4 Diag 5 Drives support Unscrew the 4 CHC M2 5x3 then remove Floppy Disk Reader See Floppy disk reader screws page 4 Diag 6 Floppy disk reader screws Unscrew the 4 CHC M2x3 screws wit
40. This drawing is the property of ABX and may not be reproduced or distributed without authorization pentra 80 Analysis cycle technology 1 Analysis cycle description Principles 4 2 2 Measuring principles 4 4 2 1 Multi distribution sytem MDSS 4 4 2 2 CBC detection Principles er de 4 5 2 3 WBC and differential count eeceeeeeneene 4 7 DIAGNOSTICS PENTRA 80 TECHNICAL MANUAL RAAO22B 1 Analysis cycle description Principles Aspiration of blood sample from the tube trough the cap 53ul e Translation of sampling carriage over Rinse chamber Cleaning of the needles e Internal cleaning 1ml inside piercing needle e External cleaning 2ml outside piercing needle e Internal cleaning 1ml inside piercing needle and reject of Sul of blood not used e Translation of sampling carriage DIL1 chamber first dilution descent of the needle e Positioning the needle point into a tangential flux of ABX DILUENT 1 65ml and synchronised distribution of 10ul of blood e Translation of sampling carriage over WBC BASO count chamber e Positioning the needle point into a tangential flux of 2ml of ABX BASOLYSE II and synchronised distribution of 10ul of blood Dilution Blood Volume 10 ul BASOLYSE II Volume 2000 ul Dilution Rate 1 200 e Translation of sampling carriage over LMNE mixi
41. V1 1 2 RAH990D technical note V1 3 2 e Pentra XL80 historic RANOO2B Technical note V1 1 1 4 4 N OF BP MDSS Technology pentra 80 Alarms amp error list Analyzer error types and help messages 6 2 Transfer error types an help messages 6 3 STAT mode error type and help messages 6 4 Environment error type and help messages 6 4 User error types and help messages 6 5 Expiration date error types and help messages 6 5 Analyzer internal barcode error types and help messa DIAGNOSTICS PENTRA 80 TECHNICAL MANUAL RAAO22B 1 Analyzer error types and help messages Table 1 Analyzer error types and help messages 2 6 Carriage motor failure Carriage mechanism not rea ching home Counting syringe motor failure Counting syringe mechanism not reaching home Diluter syringe motor failure Diluter syringe mechanism not reaching home DRAIN 1 syringe motor failure DRAIN 1 syringe mechanism not reaching home DRAIN 2 syringe motor failure DRAIN 2 syringe mechanism not reaching home Drain sensor sensor number 1 2 or 3 time out Injection syringe motor failure Injection syringe mechanism not reaching home L MNE transfer sensor time out Needle motor failure Needle mechanism not reaching home Piercing UP or DOWN bad posi tion Heagent temperature
42. XBA470A Solenoid JST 700mm CCA046A PHR 2 JST 1 Fil blanc AWG 24 2 Fil blanc AWG 24 Cosses FASTON 2 8 protege cosses Modification D signation TORON E V lg 700 JST 2pts Tol rance G n rale Etat de Surf Mati re 0 0 Matiere Traitement DIAGNOSTICS Revetement Rev tement XBA470A Folio Z A4 Ce plan est la propri t de la soci t ABX il ne peut tre reproduit ou communiqu sans autorisation Diag 42 XBA470A Solenoid JST 700mm SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 41 XBA471A Solenoid Molex 230mm 230 2 Fils AWG22 extra souple blanc 3 COLLIERS 2 4x92 5240N1 2 DBHOO1A CAKOIBA Cosses FASTON 28 protege cosses 10 00 530 x Modification Vis Des Date DM Rev Kuu TORON E V BARILLET P80 Etat de Surf Tol rance G n rale Mat i re Z2 Traitement IReve temenf Rev tement N XBALTIA Folio Z Format Ak Dim en millim tres Ce plan est la propri t de la soci t ABX il ne peut tre reproduit ou communique sans autorisation DIAGNOSTICS Diag 43 XBA471A Solenoid Molex 230mm 45 56 sues anbiunuuo no jInpoJdaJ a343 Nad au 3421205 e ayalsdoud e 453 ued wo GHS PEV Hew S VELTVEX N 9H WO 94 _E G0 S A SC quus WE gl Di oss
43. ous sauieb aun sed saunssida anbeu 29634024 92 OMY anos sj Jed unseu sabuojosd 97 abnos spy Z 18 INC 92 3600 y4 9 EEd 92 35001 yj 6 EE 91 OMY PIE I4 E E 820220 30 OMY noa pi g O2NIW 338 9EGHH 30 my 95662 y4 1 Y61032 3J NV1SIS33 1S 9 HHd 0Zz31912p oway sauieb aun sed saunssida anbey gt sabajosg 2 OMY sl 220 sabuojoad 97 OMY n lq SM Z 34n55id3 INOZ Ll S Z ano 47 Bd E HOU TZ OMY jj Z 39 waware dey L 9120220 SN3H3IS 334 9 EUW LOLLS8 N12 3 26 XBA425A Chamber heating Diag 28 XBA425A Chamber heating 30 56 ELECTRIC amp ELECTRONIC PRINCIPLES SECTION 3 UOIJEZIJOYINE JNOUJIM 51 JO panpoudas aq jou pue xgy Jo Ajsadoud ayy si siu sayaw WE 9le5S V 9215 Mig S 199us VLe VaX NS 0034 3420 8 MU buieoj5 v fes 00 30 HD 08 V91N3d 10 gt lt Le Po ESS BUY SSIUYDNOY 92U8 13 0 DJepueis 08 VYLNJd SS3NHYH ASSY DND3MH3ld 3801 ISOT uo jeubiseq v9003V VIVS 5 18 660 S 0XBZ JVA05S VS VOE0V11 VLOOAVO S 0d LL00 QHd8 vzoonvu 422 92 DMY uu EEO OL EVO LIVLNO VLEOVII ees BW zns L t 1HDIH 31G33N DNIX2H3ld O 31033N ONDH31d
44. 2 syringes vacuum control and adjustement e Required tools e Allen keys e Barflex e Flat screws drivers e Required products None e Intervention time 15 min e Frequency On request e Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 Draining syringe vacuum check No adjustment available 1 1 Draining syringe 1 Remove cover See RAS342 procedure Disconnect the tube on the draining syringe 1 inlet 1 and replace it by the Barflex tube See Diag 1 Draining syringe 1 page 2 Mn Diag 1 Draining syringe 1 Enter Menu Service Menu Technician _Gains Vacuum control See Diag 2 Vacuum control page 2 PENTRA 80 Technical Adjustment Theisen M D S S ane Liquid Sensor Adjustment Vacuum Control Sampler adjust Bubbling Counting Vacuum Value 900 Counting Syringe Drain 1 Syringe Motor Drain 2 Syringe Motor e QU A V Version V1 3 0 8 03 16 2004 09 32 59 Diag 2 Vacuum control Press Draining syringe 1 Check vacuum on the Barflex vacuum must be stable during 30 secondes Press OK button See Diag 3 Validate page 3 2 6 VACUUM ADJUSTMENT RAS334B PENTRA 80 Technical Adjustment Tam en MOSS Liquid Seed Ven Control sempre ing Counting Vacuum
45. 3 43 XBA486A LEDs extension JST 1000mm 3 47 3 44 XBAA87A LED JST 200mm 3 48 3 45 XBA488A Waste level detection 3 49 3 46 XDA605A Diluent reservoir with level detection 3 50 3 47 XDA751C 12 valves assy LV1 6 amp LV7 12 3 51 3 48 XDA752C 7 valves assy LV13 19 3 52 3 49 XDA753C Carriage valves assy LV20 23 3 53 3 50 XDA754C 7 valves assy 1 24 30 3 54 3 51 XDA755C 5 valves assy LV31 35 3 55 J Fenta 60 3 55 SECTION 3 1 Main board 1 1 Main board general view ELECTRIC amp ELECTRONIC PRINCIPLES LMNE PREAMPLI BOARD RBC PLT CIS BASO WAC CIS MEASURE zn FN ip U e e m R N ee ee A er a4 rl m m 1 5 ce B E 9 kS HGBGAIN 3 B e E 5 e i s 8 a 7 s 8 a m x ES 8 5 ay wel s mm oo Past PLI_L Threshold ET rs sS Sm p om T MS m K 3 sw wis MP m sl d Eloi ur Fo Paa ss Us GAIN m iJ 8 e e me e gro 1 Cad 1 We D UD 2 RSCGAN om Bl 4 I s a OPTICAL Er e BENCH FAN ux LMNE HET
46. 4 3 1 CBCOOBA Barcode 3 5 3 2 DADO75AS Flat cable 350mm 14pts 3 6 3 3 DAD107AS Flat cable 480mm 14pts 3 7 3 4 DAD1094A Flat cable 560mm 14pts 3 8 3 5 DAD112AS Flat cable 930mm 26pts Main board Car 3 9 3 6 DAD119AS Flat cable 950mm 8pts 3 10 3 7 DAD120A Flat cable 800mm 14pts 3 11 3 8 DAD121A Flat cable 700mm 44pts 3 12 3 9 DAD122A Flat cable 650mm 14pts 3 13 3 10 DAD123A DSUB 25pts Flat cable 1000mm 3 14 3 11 DAD124A DSUB 9pts Flat cable 1000mm 3 15 3 12 XAA428C Carriage board 3 16 3 13 XAA429A LEDs board 3 17 3 14 456 Main board 3 18 3 15 XAA458A LMNE amplifier board 3 19 3 16 XAA459A Motor board ss 3 20 3 17 XAA478A Solenoid board 3 21 3 18 XBA322B External waste level detection 3 22 3 19 XBA342A Infrared 3 23 3 20 XBA387A Reagent heating coil 3 24 3 21 XBA389A Hemoglobin photometer
47. 9 Move the rack to have the left tracer in the notch number 10 of the rack Enter Menu Service N Technician Menu Gains N Sampler adjustment Press the Check mixer button the grabber move forward with the grabbers open Check the horizontal position of the grabbers regard to the tubes See Diag 8 horizontal position page 5 The tubes have to be centered in the grabbers If it isn t the case loosen the 3 CHC mixer fixation screws then adjust this position Take care not to move the vertical position previously adjusted Upper view Grabber Left tracer Rack Diag 8horizontal position 5 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 6 6 p ntra 80 Automatic sampler RAS347B DIAGNOSTICS e Concerns e Loading mechanism check amp adjustment e Mixer mechanism check amp adjustment e Transfer mechanism check amp adjustment e Required tools None e Required products None e Intervention time 1h e Frequency On request Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 Loading mechanism check amp adjustment Remove cover except Loading Tray and Ejection Tray See RAS342 procedure Enter Menu Service Technician Menu Gains Sampler Adjustment See Diag 1 Sampler adjustment menu page 2
48. Control reference CAE10A Do not touch the TH M3x16 screw and HU M3 nut Unscrew the two CHC M2x10 screws and adjust switch to have detection in this position See Tube height control page 7 and no detection when you put a 0 3 mm gauge between TH M3x16 screw and the plate See Tube height control page 7 Diag 15 Tube height control Tube Sensor reference CAEO1 14 Unscrew the two CHC M2x10 screws and adjust switch to have detection when you push a rack with tube under the caster Keep a little play in this position See Tube sensor switch page 7 i PLAY Diag 16 Tube sensor switch 7 10 PENTRA 80 TECHNICAL MANUAL SENSORS CHECK AND ADJUSTMENT PROCEDURE Left Rack Locker Right Rack Locker reference CAE010A Enter Menu Service Technician Menu Gains Sampler Adjustment Put the rack on Loading Tray Press Check Rack Transfer Movement button Rack moves under Tracers followers then it run 25 motions on the left and 25 on the right The following screen shows the detection A and non detection B zones of the Tracer switches See Tracers detection zone page 8 A B A Sampler Utilities ae oe PN LEFT TRACER Rack motion ack e Diag 17 Tracers detection zone Non detection zone B width is depending on tracer and on switch up and down position Loosen the two CHC M2x10 screws and adjust switch position If non detection zone B is too small p
49. DBKOO9A 1 Adhesive support D 9mm EABO21A 1 Y connector Diam 3mm EABO26A 1 Y connector Diam 2 5mm 1 I connector 1 6mm 1410 6 EAB035A 1 I connector 22 mm 1220 6 EACO10A 2 Female luer connector 1 3 EAC019A 1 Male luer connector l 3 EAEOO5A 2m Tygon tube 1 02mm 0 040 2m Tygon tube 21 295mm 0 051 EAEO007A 2m Tygon tube 1 52mm 0 060 2m Tygon tube 22 06mm 0 081 EAEO11A 2m Cristal tube 3x6 EAE028A 2m Cristal tube 4x6 EAEO34A 2m Tygon tube 2 54mm 0 100 EBBO59AS 1 Filter support Swinnex 25 mm FAAO13A 1 O ring 1 8x1 2 FAAO055A 1 O ring 1 07x1 27 fluocarbon FAAO57A 1 O ring 5x1 FBHO18A 1 Housse Pentra 80 FBLOO1A 1 Rubber cap 2 holes GAK302A 3 Bottle cap GBG138A 1 1 4 turn door key 145 1 Reagent straw stopper g20 GBG155A 1 Capsule 25 JAJO11A Box 33x25x08 GBG245A 2 Reagent straw stopper 228 MABOO2A 1 Allen bent key 2 5mm MABOOSA 1 Allen bent key 1 5mm 3 10 PENTRA 80 TECHNICAL MANUAL INSTALLATION Table 2 Installation Kit XEA785B m 4 10 MAB018A 1 Allen bent key 3mm MABO90A 1 Torx bent key T10 XDA483B 1 3V liquid valve without self XDA621A 1 O ring 30 8x3 8 Washer XDA692A 1 Diluent input tubing XEAO18A 1 Diluent straw Lg 360mm XEAO19A 1 Grease XBA322B 1 Waste straw HAXOO53 1 Hack label Type A 1 to 20 HAXOO054 1 Hack label Type B 1 to 20 DAJOO7A 1 Lamp 20W 9 5V MABOO1A 1 Allen bent key 2mm HAN516A 2 Touch sc
50. DIAGNOSTICS patent Diag 3 Blood distribution in a tangential flow 4 12 SECTION 4 ANALYSIS CYCLE TECHNOLOGY 2 2 CBC detection principles 2 2 1 RBC PIt Measurement of impedance variation generated by the passage of cells through a calibrated micro aperture The specimen is diluted in an electrolytic diluent current conductor and pulled through the calibrated micro aperture Two electrodes are placed on either side of the aperture Electric current passes through the electrodes continuously When the cell passes through the aperture electric resistance between the two electrodes increases proportionately with the cell volume The generated impulses have a very low voltage which the amplification circuit increases so that the electronic system can analyze them and eliminate the background noise Analogue conversion Results Number of cells counted per volume unit x calibration coefficient Volume Data integration and plotting Histograms of ABC distribution curve RBC Distribution curves on 256 counting channels from to 300fl Plt Distribution curves on 256 channels from 2fl to a mobile threshold This threshold moves according to the microcyte population present Diag 5 RBC distribution curve in the analysis area Analogue conversion Data integration and plotting of PLT distribution curve Diag 6 Plt Distribution curve Dilutions Table 1 RBC PIt dilutions Technical c
51. Diag 15 Carriage positions values If you want to modify only one of the position remind to increase or decrease the next motion with the same number of steps not to loose the other adjustments See Diag 16 Chambers page 8 Diag 16 Chambers 8 10 PROBE ADJUSTMENT RAS330B 4 Further adjustment Use a 0 15 mm gauge 1 between the Piercing block A and the White safety block B to adjust the collar C blocking the carriage vertical movement See Diag 17 Collar adjustment page 9 Diag 17Collar adjustment When the carriage is at the Home position at 2 mm of the tooth wheel adjust the gap between the White safety block B and the Piercing block A by unscrewing and moving the White safety part See Diag 18 White safety block adjustment page 9 The gap must be adjusted between 07 mm and 1 mm Diag 18White safety block adjustment 9 10 PENTRA 80 TECHNICAL MANUAL PROCEDURE 10 10 p ntraso Main Board Adjustment Procedure RAS331B DIAGNOSTICS e Concerns Main board adjustment e How to access to Main board e Main board general view e Main board supply check e Main board adjustment e Required tools e Allen keys e Voltmeter e Required products RBC PLT latex LADOO2AS e Intervention time 30 min e Frequency On request e Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regul
52. Diag 7 End of transfer page 6 5 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE Stop Transfer Switch Ejection Tray ON PLAY 0 5mm Diag 7 End of transfer Make sure that motor does not loose steps noise at the end of transfer motion v If rack is too much on right decrease Stop Transfer value If rack is too much on left increase Stop Transfer value Repeat the procedure until you get the correct adjustment 3 3 Rack Ejection Rack Ejection value is the number of steps run by the Transfer Carriage to move Ejection Assembly Put one rack on Loading Tray and nine racks in Ejection Tray against Ejection stops to make sure it can push ten racks Press Check Rack Ejection button See Diag 1 Sampler adjustment menu page 2 At the end of cycle rack must be out of Ejection stops but ejection assembly must not be at mechanical stop See Diag 8 Rack ejection page 7 6 8 AUTOMATIC SAMPLER RAS347B EJECTION STOPS Diag 8 Rack ejection If rack is not out of Ejection stops increase Rack Ejection Value If rack is too much out of Ejection stops or at mechanichal stop decrease Rack Ejection Value Repeat the procedure until you get the correct adjustment 3 4 First Transfer asas First Transfer value represents the number of steps of the First Carriage motion Other motions are always the same representing space between two tube posi
53. E Document on Sokode 5 59 Control Panel Msdos sys DKB System file 03 20 2001 23 50 RHSA Lem I NORINLOANER tot Text Document 09 29 2003 13 43 A t 27KB MS DOS Application 05 24 2002 11 21 RHSA i 153KB File 05 24 2002 11 21 RHSA Type the name of a program folder or document and 307200 System file 03 19 2004 11 50 Windows will open it for you 1KB ID File 03 19 2004 13 13 H Eres File Folder 03 19 2004 13 14 A M Run in Separate Memory Space Cancel Browse i gt 15 obiect s 300MB Disk free space 2 82GB Diag 4Run A white window appears with a red cross in the left upper corner Press on this cross it will move to the next position Press the red cross for each position then press Enter the Touch screen is calibrated 3 2 For a Pentra XL80 Press the Launch Windows Explorer button Enter C FIDTSERV Launch Setup exe then Calwin exe A white window appears with a red cross in the left upper corner Press on this cross it will move to the next position Press the red cross for each position then press Enter the Touchs creen is calibrated 4 4 p ntra 80 Instrument Mother Board Replacement Procedure RAS354B DIAGNOSTICS e Concerns Instrument mother board replacement e Required tools e Allen Keys e Voltmeter e Required products None e Intervention time 30 min Frequency On request e Specific kit or consumables e For Pentra
54. ELECTRIC amp ELECTRONIC PRINCIPLES 3 3 DAD107AS Flat cable 480mm 14pts STANDARD VERSION HE10 F14 HE10 F14 CAHOOZA CAH002A J5 LASER BENCH BOARD Q c lt ea J 1 gt 1 LL MOLDED ON VERSION HE10 F 14 Ex 3365 14 3M HE10 F 14 MOLDED ON MOLDED ON Ex 87114 1600 3M Ex 87114 1600 3M NOTE REFER TO THE FLAT CABLE MANUFACTURING PROCEDURE RCF013A Add molded on version ADC 05 16 02 681 B Creation for RETIC optical bench ADC 01 31 97 13466 Description Chk By Date ECR Rev Designation FLAT CABLE 14 POINTS L 480 Standard tolerance Materia Material Treatment DIAGNOSTICS Coating Coating O N DADI07A_ Sheet S This drawing is the property of ABX and may be reproduced or distributed without authorization Diag 5 DADO75AS Flat cable 480mm 14pts 7 56 PENTRA 80 TECHNICAL MANUAL RAAO22B 3 4 DAD109A Flat cable 560mm 14pts VERSION STANDARD S 560 mm a LC270 J35 MASTER BOARD LC270 TOP 7 VALVES BOARD VERSION SURMOULEE S 560 mm OOO 68686868606 PEI F 12 HETO FT14 SURMOULE Conducteurs SURMOULE NOTA Se r f rer la proc dure de fabrication des limandes RCFO13A 3969 16 06 98 CREATION REMPLACE LA LIMANDE DAD075A SUR LE LC270 Modification esignation LIMANDE 14pts 7 VANNES HAUTES DADIO9A LC2 70 REF du document DAD1O9AS m N Plan
55. IS LER CICR 9 18 PENTRA 80 TECHNICAL MANUAL 4 2 Basolyse 2 circuit Bm 10 18 kI m LIN T T d RIS 6 AO D pene pe i Jm Eme iz TD DS mr 31 L 354 Sve isan wg ar XLSNISDJ XAY I RAAO22B If ABX DLUENT ka x mamaman HYDRAULIC amp PNEUMATIC PRINCIPLES 4 3 Eosinofix circuit SECTION 2 usva I 1 I 4 GISVM x eid lese ECE ono F et J Fa pam T B m pa E a d E w 1 KiSRISDI MAY f 18 05h wwviD XEM E K X e HE eee f ss qp J e PEEP GU NE 6 GORGE u E zh Ti RE m 7 2 fie CER CTE i nr 11 18 PENTRA 80 TECHNICAL MANUAL RAAO22B 4 4 Lyse circuit 1 ABX DLUENT ci y a Cnm ae T sb wa er de maar 18 354 105 XHY 1 inane ah KEY zm oe M as Es ly d w
56. OT FAOS uuu a 1 4 2 6 Hgo Imeasurelnent uu a 1 4 2 7 Counting aperture 1 4 2 8 Reagent consumption ml 1 4 3 Summary of performance data 1 5 DM Uc 1 5 Dod NO YI P TTE 1 5 oS Oda o I M M 1 5 4 Reagents specification 1 6 A ADA DILUENT T m 1 6 NM D E usss A u 1 6 o s Db VY 1 6 uuu 1 7 4 5 ABX FOSINOTF IX eee ne eee ee 1 7 DY T Tu u En rere roe ere nr nu 1 7 4 7 Waste handling precautions 1 8 OR eee 1 8 Seke Ve NMRA uu u uu 1 8 5 2 Blood specimens P 1 8 5 3 Known interfering substances DIAGNOSTICS PENTRA 80 TECHNICAL MANUAL RAAO22B 1 Technical specifications 1 1 Parameters Table 1 CBC Mode WBC HBC Hgb Hct MOV MCH MCHC HDW FADE PDW MPV PCT White Blood Cell Hed Blood Cell Hemoglobin Concentration Hematocrit Mean Corpuscular Volume Mean Corpuscular Hemoglobin Mean Corpuscular Hemoglobin Concentration Hed Distribution Width Platelets Platelets Distribution Width Mean Platelet Volume Plateletcrit Table 2 CBC 5DIFF Mode WBC LYM MON NEU EOS BAS LIC ALY RBC Hgb Hct MCV MCH MCHC RDW PET PDW MPV PCT White Blood Cell Lympho
57. Pull up FPC flat cable 2 2 m 1 3 EXE Tu s M a up D E ER 4 Uru _ h eh j Blue band of FPC cable in this position Diag 4 Floppy disk reader connection Remove Drives Support with Hard Disk Floppy Disk Reader and CD ROM Reader 3 4 PENTRA 80 TECHNICAL MANUAL PC FLOPPY DISK READER REPLACEMENT Unscrew the 4 CHC M2 5x3 then remove Floppy Disk Reader See Floppy disk reader screws page 4 Diag 5 Floppy disk reader screws Change Floppy Disk Reader Reference CBT014A and reassemble in reverse order Make sure that FPC cable is correctly plugged with the blue band in correct position See Floppy disk reader connection page 3 Make sure that the flat cable connector is correctly plugged on Hard Disk because 4 pin are free See Hard Disk connector page 4 Diag 6 Hard Disk connector 4 4 p ntra 80 PC CD ROM Drive Replacement Procedure RAS352B DIAGNOSTICS e Concerns PC CD ROM drive replacement e Required tools e Allen Keys e Required products None e Intervention time 1 hour e Frequency On request e Specific kit or consumables CD ROM Drive CBT013A Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 6 PENTRA 80 TECHNICAL MANUAL PC CD ROM DRIVE REPLACEMENT PROCEDURE Remove internal PC
58. S J 100 TI TEZ 35 LMNE flow cell_4 T 2 Xba403a LMNE flow cell 4 Wis 11 14 5 LMNE flow cell_2 1129 Xba403a LMNE flow cell 2 LV1_2 162 300 W653 LV2 2 14072 179 LMNE syringe_4 LMNE syringe_1 1072 205 LMNE flow cell_5 LMNE flow cell_7 102 10 Cap 2 05 65 Diluent reservoir_3 Diluent reservoir 3 205 610 LV10_1 EVAIDES 205 160 Reagent syringe_4 LV10_2 132 50 LV11_1 1 52 1s To deed T52 760 M22 5 LV22_1 152 50 LV23_3 2816 1 52 190 Wea Tes 102 7o Probe rinse block 1 oie 1 30 is Isolator 1 Isolator 3 1 52 215 LV20_1 2 18 SECTION 2 Table 1 Hydropneumatic connection HYD D2 760 ABX CLEANER REAGENT ABX EOSINO FIX REAGENT ABX BASO LYSE Il REA GENT ABX ALPHA LYSE REA GENT SAMPLING LMNE COUN TING LV20_2 LV23_2 LV22_2 VI 12 A2571 LV27_1 LV26_1 Reagent heater_8 LV26_2 Reagent heater_2 LV27_2 LV25_2 Abx Cleaner bottle LV8_3 IUOS LV6 1 DIEI LVG6G_2 Abx Eosinofix bottle LV9_3 82 Reagent heater_4 Abx Basolyse Il bottle 1 12_3 2 Reagent heater_9 Reagent heater_12 Abx Alphalyse bottle Vr 3 LV _2 Probe_1 Probe_1 LMNE chamber_4 M4_1 photocell DA 2 TES LMNE syringe 3 42 Ao T2 S S S S S 4 1652 300 1 02 115 1152 440 1 52 65 1 62 90 1552 200 S 1s 160 1 92 260 S 152 160 1 52 50 1 52 170 2 05 800 2 05 160 i ESZ 300 1552 180 1 52 120 2 05 800 2 05 160 1 92 550 S 192 160 2 05 800 2 05 160 o2
59. See Diag 4 SDIFF syringe screws page 4 Diag 45DIFF syringe screws Replace all the O rings Use one drop of silicon grease between 2 fingers to lubricate O rings Reassemble in reverse order 5DIFF syringe tightening torque 400 mN m 56 8 Ozf in 4 12 YEARLY MAINTENANCE RAS326B 5 Optical bench lamp replacement DAJOO7A Before dismantling optical bench lamp make sure it is cold Do not touch lamp bulb with the fingers use a piece of cloth or paper to manipulate the lamp Open the cover see RAS342 Front panel amp Covers dismantling procedure for further details Disconnect the lamp supply See Diag 5 Optical bench lamp disconnection page 5 Diag 5Optical bench lamp disconnection Unscrew the lamp fixation screws for few turns to release the lamp See Diag 6 Optical bench lamp dismantling page 5 Diag 6Optical bench lamp dismantling Turn the lamp and remove it Change the lamp Put back the fixation systme and block the screws Reconnect the lamp supply 5 12 PENTRA 80 TECHNICAL MANUAL PROCEDURE 6 LMNE flowcell coaxial replacement XEA663A Open the optical bench cover See Diag 7 Optical bench cover dismantling page 6 Diag 7 Optical bench cover dismantling Disconnect coaxial connector from the Optical bench board See Diag 8 Coaxial disconnection page 6 Disconnect the coaxial from the LMNE flowcell See Diag 8 Coaxial disconnection pag
60. TYGON TUBINGS KIT MAINTENANCE 1 YEAR P60 P80 KIT HEATER HARNESS P60 P80 KIT FLOWCELL INJECTOR TUBE P60 CABLE COAX LMNE FLOWCEL P60 80 KIT INSTALLATION P80 KIT MAINTENANCE 6 MONTHS P80 KIT PISTONS P80 KIT SPRINGS P80 KIT T ROOM FAN HARNESS P80 KIT GROUND FITTINGS P80 KIT EXTENSION CABLE PC P80 KIT MASTER V1 03 P80
61. contact with eyes skin and clothing Wear laboratory gloves when handling the product The product may be harmful if ingested The product can be absorbed through an open wound or inhalation e Emergency First aid If the eyes or skin come into contact with the reagent rinse with water If the reagent is inhaled breathe fresh air immediately If a large quantity is ingested drink water immediately and induce vomiting Call anti poison center or contact doctor e Storage conditions Stored at 18 C 65 F to 25 C 77 F away from light Product will degrade if exposed to air keep cap probe assembly securely tightened e ABX DIAGNOSTICS Part number 0906004 4 3 ABX BIOLYSE 6 10 e Function This reagent is used to lyse blood cells and determine hemoglobin concentration e Composition Quarternary ammonium chloride SECTION 1 SPECIFICATIONS e Description Colorless odorless e Physico chemical properties pH 6 65 e Handling Precautions May be dangerous Avoid contact with eyes skin and clothing Wear laboratory gloves when handling the product The product may be harmful if ingested e Mesuring Principles See user manual e Performances See user manual e Results See user manual e Directions for use See user manual e Emergency First aid If the eyes or skin come into contact with the reagent rinse with water If the product is ingested call immediately a doctor e Storage conditions Stored at 18 C 65 F
62. is the property of and may be reproduced distribufed without authorization Diag 10 DAD121A Flat cable 700mm 44pts SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 9 DAD122A Flat cable 650mm 14pts HE10 F 14 HE10 F14 CAH002A CAH002A Ce lt m r LLI 2 lt gt wa J1 VALVES BOARD NOTA REFER TO THE FLAT CABLE MANUFACTURING PROCEDURE RCF013A Creation for PENTRA 80 ADC 11 06 00 5304 Description Chk By Date ECO Rev Designation FLAT CABLE 14 POINTS L 650 VALVE BOARD PENTRA 80 Standard tolerance Material O Material Treatment DIAGNOSTICS Coating Coating S O N DAD122A_ Sheet S This drawing is the property of ABX and may not be reproduced or distributed without authorization Diag 11 DAD122A Flat cable 650mm 14pts 13 56 PENTRA 80 TECHNICAL MANUAL RAAO22B 3 10 DAD123A DSUB 25pts Flat cable 1000mm es 1000 mm O L i 000000000000 e0o0o000 0000000 l gt a D ac LLI cc a SZ 0000000000000 900000000000 PANEL PRINTER HE5 F25 UL HE5 M25 UL CAJ002A CAJ005A REFER TO THE FLAT CABLE MANUFACTURING PROCEDURE RCF013A THE CONNECTOR HES MUST BE UL RECOGNISED RTRT2 OR ECBT2 EX ITT CANNON 3M FCI Po o Description Chk By Date Rev Designation DSUB 25 POINTS FLAT CABLE L 1000 foo oo B PENTRA 80 PC P
63. or consumables e For Pentra 80 XAA553CS Kit e For Pentra XL 80 XBA596BS Kit Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 PC dismantling PC Hard Disk may contains some users specific datas Worklist Archives etc if the PC is 14 replaced these informations won t be accessible Read technical note included in the kit before replacing the PC assembly Switch the instrument off and disconnect power supply cable Remove the side and top covers of the instrument for further details refer to procedure RAS342 Front panel amp Covers dismantling Disconnect PC s accessories See Diag 1 PC disconnection page 2 e Mouse e Keyboard e RS and Printer ports e Local network Diag 1PC disconnection Disconnect the LED connector See Diag 2 LED connector page 3 2 4 INTERNAL PC REPLACEMENT RAS348B Diag 2LED connector Untight the PC fixation screws See Diag 3 Righthand side PC fixation screws amp See Diag 4 Lefthand side PC fixation screws page 4 Diag 3Righthand side PC fixation screws 3 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE Diag 4Lefthand side PC fixation screws Lift and carrefully remove the PC 2 PC installation Install the PC and tight the fixation screws See Diag 3 Ri
64. out of range Value Min amp Max Reagent Temperature sensor not connected Heagent Temperature sensor failure oampling syringe motor failure Sampling syringe mechanism not reaching home Thermostated Compartment Temperature sensor not connec ted Thermostated Compartment Temperature sensor failure Carriage Motor home switch always detected Carriage Motor Mechanism ini tialization failed Counting Syringe Motor home switch always detected Counting Syringe Motor Mecha nism initialization failed Diluter Syringe Motor home switch always detected Diluter Syringe Motor Mecha nism initialization failed DRAIN 1 Syringe Motor home switch always detected DRAIN 1 Syringe Motor Mecha nism initialization failed DRAIN 2 Syringe Motor home switch always detected DRAIN 2 Syringe Motor Mecha nism initialization failed Drain Sensor Injection Syringe Motor home switch always detected Injection Syringe Motor Mecha nism initialization failed LMNE transfer sensor Needle Motor home switch always detected Needle Motor Mechanism initiali zation failed Piercing Syringe Motor Reagent Temperature Reagent Temperature sensor Reagent Temperature sensor Sampling Syringe Motor home switch always detected Sampling Syringe Motor Mecha nism initialization failed Thermostated Compartment Temperature Thermostated Compartment Temperature Run an Auto Clean Check Motor in Service Menu Run an Auto
65. remplaced by a new one Put the Optical bench cover back After intervention check with normal fresh blood that you have a correct matrix 7 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE 8 8 p ntra 80 LMNE Balance Adjustment Procedure RAS337B DIAGNOSTICS e Concerns LMNE Balance calibration and forced calibration e Required tools None e Required products Fresh and normal blood samples 5 different samples at least e Intervention time 15min e Frequency On request e Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE This procedure must be performed on a clean instrument If the instrument is suspected to not be perfectly clean perform a Concentrated cleaning Enter Menu Service Menu technician others LMNE calibration See Diag 1 LMNE menu page 2 PENTRA 80 Others Technician LMNE Calibration vees Calibration coc ana Burn in e Sel Op TestDate GBBaso GBLmne 0 0 0 0 Atomatic Calibration GBBaso GBLmne Current Coef 1 0 86 New Coef 1 0 86 Mean 0 0 0 0 04 18 2002 12 34 50 amp ES Ic Version v0 33 Diag 1 LMNE menu Prepare a minimum of 5 different human blood samples without WBC alarm Press on Stat mode button See Diag 2
66. sit for an hour at the correct room temperature before use 1 5 Electromagnetic environment check The Pentra 80 as been designed to produce less than the required level of electromagnetic interferences in order to operate in conformity with its destination The electromagnetic interferences caused by the Pentra 80 are limited to a level allowing the correct operation of other instruments in conformity with their destination In case of problems check that the instrument is not placed in proximity of electromagnetic fields or short wave emissions radars X rays scanner etc 1 6 Environment protection Used accessories and consumables must be collected by a laboratory specialized in elimination and recycling of this kind of material according to the legislation 2 Instrument Installation 2 1 Unpacking Unpack the instrument carefully PROCEDURE RAS324B After unpacking check that all of the parts from the package list are present Table 1 Package list m XEA785B 1 Installation kit Pentra 80 XBA453A 1 Barcode reader GBDO72A 4 Lifting handles GBLO280 10 Rack 10 vials 13x82 CBKOA4A 1 Computer Mouse GBLO250 1 Keyboard drawer 7005056 1 Cardboard Box 7001020 1 20L container with LAUO15A sticker HAN524A 1 Mouse Carpet only for Pentra XL80 Table 2 Installation Kit XEA785B Qty 27 2 Wire guide DBHOO1A S Tyrap LA 2 4 L 92 DBH002A 3 Tyrap LA 3 6 L 140 DBKOO3A 1 Flat cable adhesive support
67. syringe body GBG033a e 2 XDA622A e 3 Piston GBC030A e 4_FX M3x12 screws e 5 CHC M3x12 screws e 6 Reagent syringe bottom plate GBGO0344 e 7 lyse reagent piston GBC031A e 8 O ring FAA0654 Diag 6 Reagent syringe view XDA622A O ring FAA063A Washer GBG149A A 3 Draining amp counting syringes 3 1 O ring replacement on draining syringe 1 Switch off the instrument and disconnect power supply cable Remove cover See RAS342 procedure Disconnect electrical connectors on the syringe then unscrew the 4 CHC M4x16 syringe screws just few turns to release the silent blocks See Diag 7 Draining 1 syringe dismantling page 5 9 Diag 7 Draining 1 syringe dismantling 5 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE Put a piece of absorbant paper under the syringe and disconnect all the tubes from the syringe Remove the syringe Drain the syringe pushing the piston nut up and down several times Open the syringe 4x CHC M4x16 screws on old model Rotation of the Syringe Body on new model See Diag 8 Draining 1 syringe opening page 6 Molded Syringe New model Machined Syringe Old model Diag 8 Draining 1 syringe opening Replace the O ring by the new one from the 6 month maintenance kit Use one drop silicon grease in between 2 fingers to lubricate O ring and piston Make sure the washer 4 is present on the old model then reassemble in rever
68. syringe dismantling Follow the same procedure than for Draining 1 syringe to replace O ring See above 7 0 PENTRA 80 TECHNICAL MANUAL PROCEDURE 4 Instrument cleaning Concerned assemblies All the thermostated compartment Outer surfaces of the instrument perpex covers reagent locations Waste connector plug Liquid valve push button Assemblies close to the needle Tube holder assembly Overflow trays Procedure Dilute the 12 cl bleach to 1 part of bleach for 4 of deionize water 1 5 Instrument environment must be cleaned No sponge nor cloth must be used Only absorbant paper thrown after use in contamination bins have to be used For small or sensitive assemblies use accurate drier papers All assemblies suspected to have been in contact with biohazardous material must be disinfected with diluted bleach the stainless steel must be bleached below 30 Celsius Blood stains or salt marks must be cleaned with spray detergent first Filter Clean the filter under the rinse chamber See Diag 12 filter page 8 from the pieces of cap rubber r ETTTTTTTTTTTTTTTTTTTTTTTTTTILLLILLIILI II _ Diag 12 filter Reinstall all the assemblies and setup the instrument back to its initial configuration 8 8 p iitra 80 Yearly maintenance Procedure RAS326B DIAGNOSTICS e Concerns e Samplig needle replacement e Sampling syringe O ring replac
69. the operations 1 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE Table 1 6 Month maintenance kit XEA710BS PN FAAO13A O ring 1 80x1 20 Nitrile Rinsing block 1 FAA055A O ring 1 07x1 27 Fluocarbon Rinsing block 1 FAAOS7A O ring 5x1 Nitrile Rinsing block 1 FAA065A O ring 6 30x2 40 Silicon Reagent syringe 1 XDA621A O ring 30 80x3 60 washer counting draining syringe 1 amp 2 9 XDAG22A O ring 15 54x2 62 washer Reagent syringe 4 XEAO19A oilicon grease 1 This procedure must be performed on a clean instrument If the instrument is suspected not to A be perfectly clean perform a concentrated cleaning Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all operations 1 Rinsing block ring replacement IxFAA013A 1xFAAO55A amp 1xFAA057A Switch off the instrument and disconnect power supply cable Open the right side door Move the carriage to access to the rinsing block Remove the 2 rinsing block screws See Diag 1 rinsing block page 2 Lift the locker See Diag 2 locker page 3 to free the probe and remove the rinsing block Diag 1 rinsing block 2 8 6 MONTH MAINTENANCE Diag 2 locker Use a small amount silicon grease with FAA057A O ring See Diag 3 O ring position page 3 and replace the O rings from the rinsing block Diag 3 O ring position Reassemble in reverse order Do not tigh
70. to 25 C 77 F away from light e ABX DIAGNOSTICS Part number 0906005 4 4 ABX CLEANER e Function Washing agent Composition Enzymatic solution with proteolytic action e Description Transparent liquid e Physico chemical properties Boiling point around 100 C pH 9 6 e Handling Precautions May be harmful Avoid contact with eyes skin and clothing e Mesuring Principles See user manual e Performances See user manual e Results See user manual e Directions for use See user manual e Emergency First Aid In case the product comes into contact with the eyes rinse with water If the product is ingested call a doctor immediately e Storage conditions Stored at 18 C 65 F to 25 C 77 F e ABX DIAGNOSTICS Part number 0903010 4 5 ABX EOSINOFIX e Function This reagent lyses RBCs fixes leukocytes and gives a specific coloration to eosinophils e Composition Alcoholic solution containing propylene glycol a formic dye buffers alkaline salts wetting agents and an aldehyde preservative Description Deep blue aqueous solution smells of alcohol Physico chemical properties pH 6 9 e Handling precautions Avoid contact with eyes skin and clothing Wear laboratory gloves when handling the product The product may be harmful if ingested or inhalted Keep the bottle closed when not in use e Mesuring Principles See user manual Performances See user manual Results See user manual Di
71. 00 5304 Description Chk By Date ECO Rev Designation IRED PHOTOCELL WITH 2 TABS JST L 600 Standard tolerance Material Material Treatment DIAGNOSTICS Coating N XBA432A Sheet S This drawing is the property of ABX and may not be reproduced or distributed without authorization Diag 31 XBA432A Infrared photocell 600mm 2 tabs 33 56 PENTRA 80 TECHNICAL MANUAL RAAO22B 34 56 3 30 XBA433A Infrared photocell 600mm 1 tab 26 WHITE CAX002A PHR 4 JST E 1 W WP gt lt uw LL L w Lo lt un un OF LJ 1 co a P80 J23 MOTOR BOARD CONTACT AWG 30 24 SPH 002T P0 5S CCA018A CCA020A Creation for PENTRA 80 CTH ADC 07 11 00 5304 Description Chk By Date ECO Rev Designation IRED PHOTOCELL WITH 1 TAB JST L 600 ww apama Coating N XBA433A Sheet S This drawing is the property of ABX and may not be reproduced or distributed without authorization Diag 32 XBA433A Infrared photocell 600mm 1 tab SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 31 XBA458A Switch JST 300mm CCA018A PHR 4 JST 1 Fil blanc AWG 24 3 Fil blanc AWG 24 Cosses FASTON 2 8 protege cosses ration CT DB 04 10 00 5304 Modification Vis Des Date D signation TORON SWITCH lg 300 JST Tol rance Generale Etat de Surf Matiere Traitement times evetemen
72. 01 v9e0NVo uo eubiss OvdD ddH d3u 00110122590 Diag 51 XDA753C Carriage valves assy 120 23 53 56 RAAO22B PENTRA 80 TECHNICAL MANUAL 3 REP REF GPAD D signation 1 GBGO74A SUPPORT 7 VL 2 XDA306C VANNE 2V NF W N 24 3 XDA307C VANNE3V W N 25 4 XDA307C VANNE N 26 5 XDA307C VANNE3V amp W N 27 6 XDA306C VANNE 2V NF W N 28 7 XDA306C VANNE 2V NF W N 29 B XDA307C VANNE3V AW N 30 2 9 CBD153A CUIVRE 7 ELECTROVANNES 10 CAD025A CONNECTEUR HE10MD14 UL EXTRACTEUR LONG NOTA R f rence du connecteur CADO25A Harting 0918 514 6904 3M 3314 6302 Berg 71918 114 PENTRA 80 7 VALVES ASSEMBLY CHAMBERS SIDE XDAT75AC 4 gt lt Creation SC LD 12 12 00 5304 DIAGNOSTICS Description ChkDrw Date ECR Rev Sheet 4 5 Drw size Scale iin millimeters This drawing is the property of ABX and may not be reproduced or distributed without authorization 3 50 XDA754C 7 valves assy LV24 30 Diag 52 XDA754C 7 valves assy LV24 30 54 56 ELECTRIC amp ELECTRONIC PRINCIPLES 3 51 XDA755C 5 valves assy LV31 35 SECTION 3 JNOYJIM PALNgiySip Jo paanpoJdaJ aq you pue xgy Jo Aysadoud ayy si siu ss uuu wd Q S Ev azs mag 4esus Pd 10 Pd V 106 00 20 01 aT S LLSONSYVId 00112590 yJ JS
73. 12 SECTION 4 ANALYSIS CYCLE TECHNOLOGY EOSINOPHILS With reagent action on cytoplasmic membranes the leukocytes keep their native size and only eosinophils are colored for optical separation Eosinophils will be situated in the upper part of the optical Y axis due to their strong absorbance qualities and their size which is nearly equivalent to large neutrophils NEUTROPHILS The neutrophils with their cytoplasmic granules and their generally segmented nuclei will scatter light depending on their morphological complexity A hypersegmented neutrophil will give an increased optical response with respect to a young neutrophil population which will be in the upper position of the optical axis depending on the presence of segmentation and or granules Additional parameters LIC Large Immature Cells and ALY Atypical Lymphocytes complete the panel available on the matrix The immature granulocytic cells are detected by their larger volumes and by the presence of granules which increase the intensity of the scattered light Therefore cells such as metamyelocytes will be found clearly to the right of the neutrophils and nearly at the same level Myelocytes and promyelocytes will be found in saturation position on the far right of the matrix These last three populations will be counted as LIC Large Immature Cells and their given results are included in the neutrophil value The blast cells will be found generally to the right of the monocytes
74. 16 ITF no C D 4 to 16 char AD111AD 0316 Codabar no start stop 3 to 16 char Al11 Code128 C D control without transmission EA10 no Terminator ED3 4 good reads before accepting code Exit and save configuration Diag 2 Barcode 3 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE 4 4 p ntra 80 Heater assy replacement Procedure RAS345B DIAGNOSTICS e Concerns Replacement of the reagent heating system e Required tools e Hexagonal keys e Flat screwdriver e Required products None e Intervention time Th Frequency On request e Specific kit or consumables XDA625AS Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 Heater assy dismantling Switch the instrument off and disconnect power supply cable Remove the cover and open the rigthhand side panel See procedure RAS342 Front panel amp Cover dismantling for further details Remove the lower part of thermostatic room See Diag 1 Thermostatic room dismantling page 2 Diag 1 Thermostatic room dismantling From the chamber assy disconnect the following tubes Rinse chamber Inlets 1 amp 2 Dil1 Hgb chamber Inlet 2 LMNE chamber Inlet 4 Leave the detection cell connected to the LMNE chamber RBC chamber Inlet 2 WBC Baso chamber Inlets 1 amp 4 Untight the 2 heater fixation screws S
75. 2 2 amp ig ki k o M AU B P C PANEL nv SV BW Ky T a Lr o cap diz T Hi LL ls ET T D e m lt m E S QE gt GEREEN UE MES R5232 3 E2 Alimentation du lecteur codes barres sur JS INTERNAL BARCODE m gt LI Power supply of barcode reader on J5 m am lt E z 81 E2 E2 m 1 1 kl 14 z m J5 8 J5 8 not connected m ta evorraur Diag 2Main board connections Switch on the instrument and adjust Mother Board See RAS331 Main Board Adjustment Restore settings See RAS357 Save and Restore Settings and follow Technical note included in the kit Put back the covers 3 4 PENTRA 80 TECHNICAL MANUAL INSTRUMENT MOTHER BOARD REPLACEMENT 4 4 p ritrago Motor board replacement Procedure RAS355B DIAGNOSTICS e Concerns Replacement of the motor electronic command board e Required tools e Hexagonal keys e Voltmeter e Required products None e Intervention time 30min e Frequency On request e Specific kit or consumables Motor board XAA459BS Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 2 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 How to access to motor board The motor board is located under the sampler loarde
76. 59 S 1592 60 S 1562 160 1752 800 1 52 160 139 480 1 02 205 S 1 30 20 1 30 320 130 Ino 250 1 14 85 1102 S 280 1 85 10 0 19 4 RAULIC amp PNEUMATIC PRINCIPLES T Probe rinse block_2 Sample syringe_2 LV25_3 LV27_3 EV 2623 Reagent heater_7 RBC chamber_1 Reagent heater_1 DIL1 HGB chamber_1 To WBC Baso chamber_4 LV8_1 Reagent syringe_2 LV6_3 TOS WBC Baso chamber 1 ies LV9_1 Reagent syringe_3 Reagent heater_3 LMNE chamber_3 LV12_1 Reagent syringe_5 Reagent heater_10 Reagent heater_11 WBC Baso chamber_2 LV7_1 Reagent syringe_1 DIL1 HGB chamber_2 Sample syringe_1 Sample syringe_1 M4_2 photocell LV4_1 1222 LMNE syringe_5 T4 1 Is 7 2 LMNE flow cell 6 LMNE flow cell 6 3 18 PENTRA 80 TECHNICAL MANUAL RAAO22B Table 1 Hydropneumatic connection ele E1_1 anode fitting E1_2 anode fitting 1192 t Isolator 1 lsolator_2 52 80 E2 1 ground fitting E2 2 ground fitting 335 LV28_2 LV28_1 52 120 T0 2 T6_1 SZ 160 o Reagent heater_5 Reagent heater_6 5 S 160 LMNE chamber_2 a s 1 52 200 RBC chamber_3 RBC chamber_2 152 480 u BAS 152 7O Count syringe_2 WASTE Rinse chamber_3 2 05 20 Filter_1 Filter_2 2 05 100 Bro 1 55 30 TO nh 62 65 Be LV32_1 1 9 15 eae 121 152 5 ome RBC chamber_4 1 52 65 LV34_2 LV34_1 152 Js 1131 1 52 180 M2_1 photocell M2_2 photocell 152 15 Isolator 2_1 Isolator 2_2 209 280 LV30_1 V50 5 5 254 160 o Drain syrin
77. 6 screws Washers and lift Hard disk CD ROM Drive Floppy Reader assy See Hd assy screws page 2 Diag 2 Hd assy screws 2 6 PROCEDURE RAS349B Disconnect flat cable from Hard Disk then from CD ROM Drive See Flat cable disconnection page 3 om WEN 2 i cer aj risi Hak Diag 3 Flat cable disconnection Disconnect FPC cable from Floppy disk Reader and remove Drives Support with Hard Disk Floppy Disk Reader and CD ROM Reader See Floppy disk reader connection page 9 Pull gently brown part of connector up 1 Pull up FPC flat cable 2 ee C EET UR MEC Op G w rit eres prey 1 17 Blue band of FPC cable in this position Diag 4 Floppy disk reader connection 3 6 PENTRA 80 TECHNICAL MANUAL PC HARD DISK REPLACEMENT PROCEDURE Unscrew the 4 CHC M3x4 screws and remove Drives Support with Hard Disk See Drives support page 4 Diag 5 Drives support Return Drives Support and unscrew the 4 CHC M3x4 screws to free Hard Disk See hard disk screws page 4 Diag 6 hard disk screws Remove Drives Support See Hard disk page 4 Diag 7 Hard disk 4 6 PROCEDURE RAS349B Change Hard Disk Hard Disk reference XAA510AS is formated and updated with last version of software Reassemble reverse order Make sure that the flat cable connector is corre
78. 80 XAA544AS Kit e For Pentra XL80 XAA545AS Kit Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 4 PENTRA 80 TECHNICAL MANUAL INSTRUMENT MOTHER BOARD REPLACEMENT Before changing a Mother Board make sure that Analyser Settings are saved on a floppy disk or on Hard disk If not follow RAS357 Procedure Save and Restore Settings to save and or print Analyser Settings Read technical note included in the kit before replacing the Mother board 1 Mother Board Acces Mother board is located on righthand side of the instrument at the rear e Switch the instrument off and disconnect power supply cable e Remove Right Front Cover RemoveThermic Panel e Remove Right side cover e Remove the Plastic Protection by loosening the two CHC M4x6 Screws For further details refer to procedure RAS342 Front panel amp Covers dismantling 2 Mother Board Dismantling Carefully disconnect all the connectors from the mother board Unscrew the 9 CHC M4x 6 Screws See Mother board srews page 2 1 A 5 e D ES k F Diag 1 Mother board srews Remove Mother Board 2 4 PROCEDURE 3 Mother Board Replacement LMNE PREAMPLI BOARD RBC PLT CIS BASO WBC CIS MEASURE 1i fe e ma 518 Eis e um xm adu L 4 e a HGB GA
79. 80 DAD119A CABLE RACK TRANSE SOLENOID P80 DAD120A CABLE PREAMP BOARD LMNE P80 DAD121A CABLE MOTOR PCB MAIN PCB P80 DAD122A CABLE LIQUID VALVE L 650 10F14 DAD123A CABLE PC PRINT L 1000 5 25 P80 DAD124A CABLE PC RS L 1000 5 09 P80 DAJOO7A OPTICAL LAMP FOR BENCH P60 P80 DAKO26A FAN FAN FOR SOCKET 370 PC P80 DAMOO9A VALVE SOLENOID PIERC BLOCK DBNOO6A PCB POWER SUPPLY BLOCK P80 EACOO8A FITTING ANTI ROTATION WASHER EACO10A FITTING LUER FEMALE I 3MM EAEO05AS TUBING TYGON 1 016 0 040 L 2M EAEOO6AS TUBING TYGON 1 295 0 051 L 2M FAE007AS TUBING TYGON 1 52 0 060 L 2M EAEO08AS TUBING TYGON 2 06 0 081 L 2M EAEO09AS TUBING TYGON 2 29 0 090 L 2M EAEO28AS TUBING CRYSTAL 4X6 L 2M TUBING TYGON 1 143 0 045 L 2M EAEO34AS TUBING TYGON 2 54 0 100 L 2M EBBO59AS FILTER RUBBER CAP DEBRIS 25mm FAAO13A O RING NEEDLE RINSE BLOC C 80 FAA040A O RING 5DIFF SYRINGE D 12 1 SECTION 09 SPARE PARTS LIST Table 1 Spare part list FAA054A O RING SAMPL NEEDLE C P80 FAAO57A O RING PIERC NEEDLE P120 C 80 FAAO064A O RING SAMPL SYRINGE P60 P80 FAAO065A O RING REAGENT SYRINGE D 6 3 FAAO066A O RING DRAINING CHAMBER P60 80 FAAO067A O RING SDIFF SYRINGE D 2 4 FAJOOSA COVER CLOSING MAGNET P80 FAKOO1A CHAMBER APERTURE 50u CHAMBER APERTURE 80 FALOO9A SILENT BLOC FOR SUB ASSEMBLIES FALO10A SILENT BLOC OPTIC BENCH P60 80 FBHO18A COVER DUST COVER P80 FBLOO1A HEAGENT REAGENT CAP 2 HOLES FBMOOSA COVER REAGENT COVER HOLDER P
80. 80 FBRO11A BELT NEEDLE L 364 P60 P80 FBRO20A BELT CARRIAGE L 660 P80 GALO94A CHAMBER WAST P120 DIL TANK P60 GBCO15A CLIR MIX CH HOLDER MIC P120 GBCO30A SYRINGE REAG PISTON P60 SPS 80 GBC031A SYRINGE LYSE PISTON MIC P60 80 GBG033A SYRINGE REAG BLOC BODY P60 P80 GBG037A SYRINGE SDIFF BLOC BODY P60 80 GBGO40A SYRINGE SDIFF PISTON P60 P80 GBG042A SYRINGE CROSSPIECE P60 SPS P80 GBG044A SYRINGE SAMPL SYR BODY P60 80 GBG048A SYRINGE SAMPLING CROSSPIECE XEA747AS SYRINGE VAC WASTE PISTON P60 GBG145A REAGENT STRAW D 20 P60 P80 GBG275A O RING APERTURE P60 M60 P80 GBG157A CHAMBER COUNTING HEAD P60 P80 GBG166A NEEDLE PIERC RINSE BLOC C 80 GBG278A NEEDLE RINSING BLOCK P60C P80 GBG169A NEEDLE PIERCING P60C P80 GBG288A NEEDLE GUIDE P60C P80 GBG176AS CARRIAGE NEEDLE STOP P60C P80 GBG211A SYRINGE VACCUM PUMPBODY P60 80 GBG212A SYRINGE WASTE PUMP BODY P60 80 GBG245A REAGENT STRAW D 28 P60 P80 ACT GBLO042 COVER RACK LOADING TRAY P80 GBLO062 CARRIAGE RACK EJEC PUSHROD P80 GBLOO68 COVER RACK EJECTION TRAY P80 GBLOO089 CUP OVERFLOW T ROOM P80 GBL0149 CUP FOR GRABBER P80 GBL0164 CUP OVERFLOW REAG BOTTLE P80 GBL0183S SAMPLING STD TUBE HOLDER P80 GBL0193 CUP OVERFLOW LIQ SYRINGES P80 GBL0213 CUP OVERFLOW MAIN BOARD P80 3 6 PENTRA 80 TECHNICAL MANUAL 4 6 RAAO22B Table 1 Spare part list Is GBL0216 GBL0254S GBL0280 HAEO26B HAEO27B HAEO28B HAEO29B HAX0032 HAX0033 HAX0034 HAX0053 HAX0054 HAX0055 HAX0056 HA
81. 9 4 1 tubing EAEO48A 1 85 10 I tubing S2x4 Lg 6 tubing Sleevings GALO98A 30 EAE026A Tube shielding XBA403A 2 3 Function of valves Table 4 Function of valves Valve Subject Functions number 1 Differencial diluent Selects flowcell sheath 2 or LMNE chamber 2 Differencial diluent Differencial diluent Selects flowcell sheath 1 or sheath 2 4 Ee mee Opens pathway from LMNE chamber to flowcell supply Flowcell sample injector Opens waste path for sample LMNE syringe 6 ee Selects rinse for probe rinse block or WBC Baso chamber i Alphalyse Control alphalyse entry vexit reagent syringe 7 18 PENTRA 80 TECHNICAL MANUAL 8 18 Table 4 Function of valves RAAO22B Valve pee Subject Functions number 8 9 10 11 12 13 14 15 16 17 18 18 20 21 22 23 24 25 26 2 28 29 30 31 32 33 34 35 Cleaner Eosinofix Diluent Diluent ouput con trol Basolyse Il Flowcell rinse Counting syringe WBC and RBC PI count Counting syringe Draining syringe 2 Draining syringe 2 Diluent reservoir Probe Probe Probe Probe Draining syringe 1 WBC Baso Coun ting head Diluent chamber select Diluent circuit Flowcell drain Diluent circuit Counting syringe Chambers Chambers Chambers Chambers Chambers 4 Hydraulic circuit Control cleaner entry exit reagent syringe Control eosinofix entry exit reagent syringe Control alphalyse entry exit reag
82. ADJUSTMENT RAS333B Make sure the thermometer sensor is deeply plunged into the liquid After thermometer probe is installed close the thermal door Enter in Run Cycles Number field in the Reagent heating area of the window 5 cycles to run Press Check Thermic in the Reagent heating area of the window button to start control At every bip your ear from the machine check the temperature is around 35 C 1 C Check is validated if you get during control at least 10 times the right temperature If an adjustment is necessary when heating block has been changed for example perform the following point of this procedure 1 2 Reagent heating coil temperature adjustment Check and eventually change the CTN value See Main Board Reagent heating coil CTN value page 3 Diag 3Main Board Reagent heating coil CTN value The Heating coil CTN value is located on the CTN wire close to the main board connected on 14 J31 Be sure to read the correct value as the sticker may be or down Adjust as described for the control the temperature by the mean of the Temperature recorded value in the Thermic adjustment window See Thermic adjustment window page 2 Temperature recorded value in the Reagent heating area of the window must be rounded 34 35 36 e If Temperature recorded is increased reagents preheating temperature increases e If Temperature recorded is decreased reagents preheating temperature
83. ANUAL REP REF GPAD D signation 1 GBGOT4A TOLE SUPPORT 7 VL 2 XDA306C VANNE 2V NF 4W N 13 3 XDA306C VANNE 2V NF W N 14 amp XDA306C VANNE 2V NF 4W N 15 5 XDA306C VANNE 2V NF W N 16 6 XDA306C VANNE 2V NF amp W N 17 SS 7 XDA307C VANNE3V 4W N 18 Se f XDA307C AW N 19 10 CAD025A CONNECTEUR HE10MD14 UL EXTRACTEUR LONG NOTA R f rence du connecteur CAD025A Harting 0918 514 6904 3M 3314 6302 Berg 71918 114 7 VALVES ASSEMBLY SYRINGES SIDE XDAT52C 4 gt lt a PENTRA 80 Creation SC LD 12 12 00 5304 A DIAGNOSTICS Description ChkDrw Date ECR Rev Sheet 2 5 Drw size A3 Scale mi millimeters This drawing is the property of ABX and may not be reproduced or distributed without authorization 3 48 XDA752C 7 valves assy LV13 19 Diag 50 XDA752C 7 valves assy LV13 19 52 56 ELECTRIC amp ELECTRONIC PRINCIPLES SECTION 3 3 49 XDA753C Carriage valves assy LV20 23 pnoy PalNqi4sip paanpoadeu jou xgy Jo Ajuadoud ayy sI sip saunu w E wes Ev azs Mag S E 43345 S91LSONOVIA 08 VMIN3d p JESLV UX ATHWASSV S3ATVA 7 SINNVA LOlHVH2 31HVO 2825vVX 9 EZAN MY AE3NNVA _ 2L0EVOX S ZEN MY AEJNNVA 2L0EVOX LZN M JN AZ INNVA 290tVOX 02 M AZ INNVA _ 290 z Y LHOddnS 31
84. ASO Number of cells per volume x coefficient of calibration in percentage regarding the total number of leukocytes BASO WBC nuclei SECTION 4 2 3 3 LMNE Matrix The WBC and Differential count are based on 3 essential principles e The double hydrodynamic sleeving DHSS ABX DIAGNOSTICS patent e The volume measurement impedance changes e The measurement of transmitted light with 0 angle which permits a response according to the internal structure of each element and its absorbance by means of incident light diffusion 25ul of whole blood is delivered to the LMNE chamber in a flow of EOSINOFIX This reagent lyses the RBC stabilizes the WBC in their native forms and stains the eosinophil nuclei with a specific coloration The solution is then stabilized with diluent and transferred to the measuring chamber Each cell is measured both in absorbance cytochemistry and resistivity volume Table 4 WBC counts ANALYSIS CYCLE TECHNOLOGY light absorban measurement Diag 9 DHSS principles Technical characteristics of the WBC counts during the acquisition of the matrix Initial blood volume 25 yl Method Impedance with hydrofocus Vol ABX Eosinofix 1000 ul Aperture diameter 60 um Diluent Volume 1000 ul Flow diameter 42 um Final dilution rate 1 80 Injection duration Temperature of reaction 5 Volume injected 72ul Incubation duration 12s 9 12 PENTRA 80 TECHNICAL MANUAL RAAO22B
85. CEDURE 3 Print Enter Menu Settings Press the Print Send button A the following window appears See Diag 7 Settings print window page 4 IDAE CA wx gt y En s Diag 7Settings print window Select an option then press the Validate button 3 1 Print analyzer settings This option allows to print Mechanical Systems Technical adjustment parameters Pulse Adjustment parameters Daily workload parameters Quality assurance parameters 3 2 Print settings This option allows to print The analyzer identification The Reagents configuration Quality assurance configuration and parameters The printer settings The cycle options The communication configuration The type parametrings 3 3 Print both Allow to print the two previous options 4 4 MDSS pentra 80 Exploded views LA IVC io v 8 3 2 8 4 Oe 8 5 TS 8 6 8 7 6 Sy INS CASS epei 8 8 eu dean 8 9 8 Needle Rinse block 8 10 9 Needle Sampling 8 11 10 Syringe optical DEB 8 12 11 Sampling syringe assy 8 13 12 Draining Counting syringe piston 8 14 13 Draining syringe assy 8 15 14 Counting syringe body
86. CEDURE PENTRA 80 A Please Sample Latex Diag 4 Sample the latex When optical bench is well adjusted See Diag 5 LMNE adjustment screen page 4 PENTRA 80 Technical Measurement Gain Adjustment LMNE Adjustment Aperture Current Pulse Adjustment Bun Adjustment Lamp Voltage LMNE Lamp Volts en 4 8 Resistive Gain LMNE Res ee 51 00 5 70 LMNE Abs Gain LMNE Abs 10 240 Average Time of Transfer LMNE Transfer EE 90 00 100 300 Version 0 33 LMNE_ADJUST EBEEBHHSEBZEZENH 04 25 2002 15 23 27 Diag 5 LMNE adjustment screen Check that the displayed values are within the range See Table 1 TARGET VALUES page 4 Table 1 TARGET VALUES PARAMETER TARGET VALUE RANGE LMNE Lamp 6 00V 55010 0 50 LMNE res 50 AE OI ES LMNE Abs 170 Set to maximum LMNE Transfer 200ms 150 to 250 If the values are out of range or to get a better adjustment follow the LMNE flowcell adjustment procedure 4 8 LMNE FLOWCELL ADJUSTMENT RAS336B 2 LMNE Flowcell position adjustment Enter Menu service menu technician measurment LMNE adjustment Click on Run LMNE Adjustment button Closed tube holder when the following screen appears See Diag 6 Sample the latex page 5 PENTRA 80 A Please Sample Latex Diag 6 Sample the latex During the cycle adjust LMNE lamp voltage around 6V by turning R414 potenti
87. Calibration Cycles Calibration contin Blank Values System Tools in cycle Barcode Setup Edit Codes Code Type s disabled Code Length Code 39 Codabar 2 5 Interleaved Reading Direction meme Code 128 None C Transmit Check Digit Ce only C Not Transmitted T Wotteansmitted WR only Transmit Start Stop Disabled v Codabar Start Stop Char Small Letters Y l 73 34 cl IO Diag 3Barcode configuration screen 4 6 INTERNAL BARCODE ADJUSTMENT RAS343B open the tab 2 e Select the Code type Code 128 e Check the Check Digit Not transmitted box e Confirm pressing Accept values e And then open the tab 3 e Select the Code type Codabar e Check the Check Digit Not transmitted box e Confirm pressing Accept values 3 3 Settings parameters meaning Code Type This parameter is set according to the barcode typology used in the laboratory If several barcode labels are used each one must be defined in one of the tab provided in the screen Code length the recommended value is 01 16 e Reading direction can not be configured it means that the label is readable whatever its position on the tube right way or reverse way e Check Digit This parameter is set according to the barcode typology used in the laboratory e None must be checked if the barcode model is without Check Digit Trans
88. Clean Check Motor in Service Menu Run an Auto Clean Check Motor in Service Menu Run an Auto Clean Check Motor in Service Menu Run an Auto Clean Check Motor in Service Menu Run an Auto Clean Check Motor in Service Menu Run an Auto Clean Check Motor in Service Menu Run an Auto Clean Check Motor in Service Menu Run an Auto Clean Check Motor in Service Menu Run an Auto Clean Check Motor in Service Menu Run an Auto Clean Run an Auto Clean Check Motor in Service Menu Run an Auto Clean Check Motor in Service Menu Run an Auto Clean Run an Auto Clean Check Motor in Service Menu Run an Auto Clean Check Motor in Service Menu Run an Auto Clean Check Motor in Service Menu Run an initialization Run an initialization Run an initialization Run an Auto Clean Check Motor in Service Menu Run an Auto Clean Check Motor in Service Menu Run an initialization Run an initialization SECTION 6 Table 1 Analyzer error types and help messages Thermostated Compartment temperature out of range Value Min amp Max Thermostated Compartment Temperature 2 Transfer error types an help messages Table 2 Transfer error types and help messages Loading motor mechanism not reaching home Loading motor failure Stop rack loading switch not detected Stop rack loading switch detec ted Sampler Transfer mechanism not reaching home Sampler Transfer motor failure Stop rack Transfer switch not
89. Com error on slave 96d Communication error with slave an Initialization 6 6 Error management failed Error on cycle d Error on start internal chrono Hgb Blank Error Management Home Motor d error Incorrect pos motor carriage d Min d Max d Incorrect pos motor counting d Min d Max d Incor rect pos motor PRESSURE 96d Min 96d Max 96d Incorrect pos motor diluter 96d Min 96d Max d Incorrect pos motor drain 1 96d Min 96d Max 96d Incorrect pos motor FLUSH 96d Min 96d Max 96d Incorrect pos motor drain 2 96d Min 96d Max 96d Incorrect pos motor injector 96d Min 96d Max 96d Incorrect pos motor loading 96d Min 96d Max 96d Incorrect pos motor mixer 96d Min 96d Max d Incorrect pos motor needle 96d Min 96d Max 96d Incorrect pos motor sampling 96d Min 96d Max d Incorrect pos motor tranfer 96d Min 96d Max 96d Internal Barcode Error Internal synchronization failed Motor 96d is busy Run a new cycle while analyzer is busy Valve already activated Unkwon cycle Hgb blank cycle incorrect Carriage motor bad position Pressure motor bad position Diluter motor bad position Flush motor bad position DRAINING_2 motor bad posi tion Injector motor bad position Loader motor bad position Mixer motor bad position Needle motor bad position Sampling motor bad position Translation motor
90. Composition Stabilized saline solution which contains an organic buffer an antiseptic and Sodium Azide 0 196 Description Limpid and odourless aqueous solution e Physico chemical properties Boiling point About 100 C pH neutral e Mesuring Principles See user manual e Performances See user manual e Results See user manual e Directions for use See user manual e Handling Precautions Avoid skin and eye contact Use laboratory gloves when handling the reagents If a large quantity of reagent is ingested a mucous irritation can result e Emergency First aid If the eyes or skin come into contact with the reagent rinse thoroughly with water If a large quantity is ingested drink water immediately and induce vomiting e Storage conditions Stored at 18 C 65 F to 25 C 77 F away from light ABX DIAGNOSTICS Part number 0901020 4 2 ABX LYSE e Function This reagent is used to lyse blood cells and determine hemoglobin concentration e Composition The reagent contains potassium cyanide at 0 03 a quarternary ammonium salt and a saline phosphate buffer containing sodium azide lt 0 1 e Description Aqueous solution limpid e Physico chemical properties Boiling point approximately 100 C pH basic smells of cyanide e Mesuring Principles See user manual e Performances See user manual e Results See user manual e Directions for use See user manual e Handling Precautions May be dangerous Avoid
91. DURE 1 Sensor check Enter Menu Service Super User Menu Mechanical Check Sensors See Check sensors screen page 2 PENTRA 80 Mechanical System Sampler Test Holder Adjustment Rack Adjustment Initialization Check Motors Check Valves Check Sensors Sampler Transfer Rail Tube Height Control Front Cover C Tube Sensor Thermic Panel Left Rack Locker Right Rack Locker Diag 1 Check sensors screen 1 1 Loading area Remove Left Front Cover See RAS342 to have acces to switches Front cover status becomes red on previous screen Stop Rack Loading Sensor Left Push on sensor and check that Stop Rack Loading Sensor Left status becomes red See Stop rack loading sensor left page 2 C Stop Rack Loading Sensor Left Diag 2 Stop rack loading sensor left Stop Rack Loading Sensor Right Push on sensor and check that Stop Rack Loading Sensor Right status becomes red See Stop rack loading sensor right page 3 2 10 PROCEDURE RAS356B Stop Rack Loading Sensor Right Diag 3 Stop rack loading sensor right 1 2 Sampler Transfer Rail Tube Height Control Push on sensor lever and check that Tube Height Control status becomes red See Tube heigt control page 3 Tube Height Control Diag 4Tube heigt control Tube Sensor Push on sensor lever and check that Tube Sensor status becomes red See Tube sensor page 3
92. E PLT HC11 RBC HEI BASO WBC Em Eje Acquisition CPLD ma gj B PIC 16 Dual Stepper Motor Control E Sampl syringe mater current CARRIAGE External Reset tT BO32 BOARD ms i Verti carriage motor current i MOTORS mn BOARD g R5232 1 LEDS CPU CPLO CPU CPLD BOARD 3 4M 5 hod uas JTAG EMERGENCY POSIT ASSY VALVES PIC 16 cs T DRAINING 1 VALVES au gi DETECT 81 12 LJ Ld Sensor Rx DRAINING 2 VALVES Imam DETECT 24 30 g COUNTING DETECT VALVES 431 35 TRANSFERT DETECT VALVES 5232 2 PC PANEL 5 gt SUPPLY R5232 TI t2 FUMER SUE js Gs ee G a e z zle im h Alimentation du lecteur codes barres sur J5 INTERNAL BARCODE m gt JE E Power supply of barcode reader on J5 qm i rm I x 5 8 85 E2 E m P 1 1 m rE HA 25 8 25 8 n connected 3 BvDEAUT Diag 1 Main board general view 3 56 PENTRA 80 TECHNICAL MANUAL RAAO22B 2 Motor board 2 1 Motor board general view HAIN BOARD POWER SUPPLY p eu E REAGENT HE E a IT 5 MOTOR HOME SYRINGES ud Reagent syr molor current c F La o o some 0 L8 a asema Injector syr motor current 9 n _ am t FP COUNTING T m 72 fe IT_3 MOTOR HOME ILang i E Counti
93. EDURE 1 Preparation Switch the instrument on Open the thermal door Righthand side of the instrument Perform an Concentrated Cleaning from menu Service Menu SuperUser Hydraulical Clean Cycles Confirm the concentrated cleaning by pressing Ok button When asked during the concentrated cleaning cycle poor 3ml of Minoclair in each chamber then click ok to continue 5 Li h Diag 1Poor Minoclair When cycle is finished switch the instrument off and remove the power supply cable Remove the instrument s covers See procedure RAS342 Spray the bactericidal cleaner on all biohazardous areas assemblies in contact with the biohazardous material such as instrument cover tube holder keyboard and waits for 10 minutes 2 Manual decontamination Dilute the 12 bleach to 1 part of deionize water 1 5 Instrument environment must be cleaned and decontaminated No sponge nor cloth must be used only absorbant paper thrown after use in contamination bins have to be used For small or sensitive assemblies use accurate drier papers All assemblies suspected to have been in contact with biohazardous material must be disinfected with diluted bleach the stainless steel must be bleached below 30 C Blood stains or salt marks must be cleaned with spray detergent first Concerned assemblies e Outer surfaces of the instrument perpex covers reagent locations e Waste connector plug e Liquid valve push butt
94. ENT PROCEDURE 6 6 p ntra 80 PC Floppy Disk Reader Replacement Procedure RAS351B DIAGNOSTICS e Concerns PC floppy disk reader replacement e Required tools Allen Keys e Required products None e Intervention time 1 hour e Frequency On request e Specific kit or consumables Floppy disk reader CBTO14A Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 4 PENTRA 80 TECHNICAL MANUAL PC FLOPPY DISK READER REPLACEMENT Remove internal PC See RAS348 Unscrew the 6 CHC 4 6 screws to remove Internal PC from the cover Unscrew the 4 CHC M3x6 and the 4 CHC 4 6 screws See Cover plate fan page 2 Do not remove Left Angle Plate A in order to keep the PC Cover adjustment A Diag 1 Cover plate fan Disconnect fan and remove cover plate Unscrew the 4 CHC M3x6 screws M3 Washers and lift Hard disk CD ROM Drive Floppy Reader assy See Hd assy screws page 2 Diag 2 Hd assy screws Disconnect flat cable from Hard Disk then from CD ROM Drive See Flat cable disconnection page 3 2 4 PROCEDURE RAS351B j 2 EN eana x araben divis Fu Diag 3 Flat cable disconnection Disconnect FPC cable from Floppy disk Reader See Floppy disk reader connection page 3 pull gently brown part of connector up 1
95. ENVHO ausovax 08d WOON 1 MO THH3AO TIVINS HOLV 1OSI YAENVHO v9 woX 1 DESIGNATION MOTOR SAMPLING MECHA ASSY P80 NDEX PART NUMBER En XDA725A DESIGNATION MOTOR SAMPLING MECHANISM ASSY P80 C DATE 29 01 02 Cc O PENTRA 80 MODIFICATIONS NOTE TECHNIQUE ci t ABX il ne peut tre r t la propri t de la so Ce plan es 2 lt 2 72 SAMPLING STD TUBE HOLDER P80 SAMPLING OPT TUBE HOLDER P80 SAMPLING TUBE HOLDER P80 GBL0183AS DATE 14 01 02 NDEX PART NUMBER DESIGNATION 1 v lt va Creation PENTRA 80 ind NOTE TECHNIQUE MODIFICATIONS Ce plan est la propri t de la societe ABX il ne peut tre reproduit ou communiqu sans autorisation uorjesiJojne sues anbiunwwod no yinposdas ynad au Xgy 205 ej ayaisdosd ue d 08 VHJLNdd GEO INT 08d MOO1d SNIOH3Id ONIIdNIVS SNOILV JISIGOW JQOINH231 310N PU NOIL VNOIS 3G 08d 49018 SNIOMH3Id ONMIdNVS V98cZ4VOX NOILLVNOIS3Q dTaNNN LYVd X3aNi sues anbiunwwo no jinpoada asya au Ji XgV 3421205 e ap ayaisdoud ej a 534 SCORE SNOIL V2IJIQOM JNOINHDIL 3LON Pu YV 1 0g 10Vd c002 0 6L 31 VQ Ov 08 VdH LN3d 1 138 NOIL VNDIS 30 08 09
96. GB 43 24 LMNE Threshold 3 15 00 PLT Concentrate PLT Min 15 PLT 256 58 5 00 6 00 PLT Concentrate Square Coeff WBC Square Coeff RDW 0 34 PLT Concentrate Cube Coeff 8 00 WBC Cube Coeff 6 00 MPY 1 00 PLT Concentrate Division Coeff 1025 00 WBC Division Coeff 102 00 Accept Values Accept Values Version V 12 30 n Diag 7 Calibration coefficients 4 4 p iitra Tube holder adjustment Procedure RAS338B DIAGNOSTICS e Concerns Tube holder general view Tube holder assembly replacement Tube holder adjustments Needle adjustment Adjustment check Compatible tube list e Required tools e Allen keys e Flat screwdriver e Required products None e Intervention time Th e Frequency On request e Specific kit or consumables Tube holder assembly XDA728A Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 10 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 Tube holder general view Diag 1 Tube holder exploded view 2 Tube holder assembly replacement Switch the instrument off Remove the 2 front doors Push electromagnet axis to free and open the tube holder remove the tube holder Cut the 2 tyraps and disconnect the electro magnet command See Diag 2 Tube holder dimantling page 3 Before dismantling the tube holder mark with a pen the location of the tube holder support Example Red
97. HES Mixer motor current LG Jaxi Jig yey OL Jig 39JSU031 IPY OL Id LIS i pul pir d e R F E z z z t ce 8 2 a z un s ER m Po c ma Diag 1 Motor board general view 3 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE 3 Motor board check and adjustment Table 1 Motor board check and adjustment target values Reagent syringe R16 4V 0 05V Optical bench syringe R23 ov 2005 Counting syringe ies R38 4 8V 0 05V for Molded Syringe New 4 0V 0 05V for Machined Syringe Old Draining syringe 2 R50 4 8V 0 05V for Molded Syringe New 4 0V 0 05V for Machined Syringe Old Horizontal carriage hee R59 OV Olay Draining syringe 1 TP6 R73 4 8V 0 05V for Molded Syringe New 4 0V 0 05V for Machined Syringe Old Sampler TP7 R92 3 5V 0 05V Loader TP8 R110 hoy 2006 Mixer u step motor TPO R122 0 6V 0 05V This motor current new value is only applicable on new motorisation assemblies in case a new piston is installed on an old syringe assembly motor syringe the motor current remains 4V 0 05V 4 4 p ntra 80 Temperature Adjustment Procedure RAS333B DIAGNOSTICS e Concerns Reagent heating coil and Thermostatic compartment temperature adjustment e Required tools e Thermometer e Required products None e Intervention time 30 min e Frequency On reques
98. HNICAL MANUAL RAAO22B 38 56 3 34 XBA461A Switch Jst 700mm CCA018A PHR 4 JST 1 Fil blanc AWG 24 3 Fil Blanc AWG 24 Cosses FASTON 2 8 protege cosses Modification Matiere Traitement N XBA461A Folio Z Format A amp Ech 1 3 0 en millim tres Ce plan est la propriet de la soci t ABX il ne peut tre reproduit ou communiqu sans autorisation DIAGNOSTICS Diag 36 XBA461A Switch Jst 700mm SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 35 XBA462A Motor Jst 300mm 24 WHITE 2 2 24 WHITE 24 WHITE P o 5 24 WHITE CCA043A 6 EHR 4 CCA0AAA EHR 6 Ref JST EAR 4 3 COLLIERS 2 4x92 CCA045A DBH001A Contacts SEH 001T PD 6 Ref JST EHR 6 CCA045A Contacts SEH 001T P Creation CT DB 06 10 00 5304 A Modification VisiDes Date DM Rev D signation TORON MOTEUR lg 300 JST Tol rance G n rale Etat de BUM Mati re Traitement Revetement N XBA4 amp 62A Folio Z Format A4 Ech Dim en millim tres Ce plan est la propri t de la soci t ABX il ne peut tre reproduit ou communiqu sans autorisation DI ICS Diag 37 XBA462A Motor Jst 300mm 39 56 PENTRA 80 TECHNICAL MANUAL RAAO22B 3 36 XBA463A Motor Jst 400mm 24 WHITE G 5 1 2 2 2 WHITE 3 c o 3 24 WHITE o 5 24
99. IL VNDIS 3Q 08d 909d 9NIOM3Id 3 1Q033N V691999 ws 08d 909d 9014 ASNIY OH3ld 3 1Q033N 991999 EN 08d O O0ZL d 31Q033N 9NIOH3Id SNIH O V S0VVJ 08 9 MOO 18 ASNIY 3 IO33N ONIAO VelLovva EB 08d 909d 42018 ONISNIY 31Q033N 891999 EN 9 19 3 IO33N IdlNVS ONISH O Vvsovva EN 08d O09d ACIND 3 IO 33N V0 19949 ER NOLLVNO9IS3CG 38IAnN LAVd XANI UOIJESIJOJNE sues SNDIUNWWOD no JInpoJda J 3343 nad au X8V 2 ej ajaludoud ej ue d 60 39Vd Ovid 31V0 EN 08 09 3 IQ33N SNI TdINVS 31Q033N NOIL VNDIS3Q 08 09d 31Q33N SNI TdINVS 31033N SVSS9VGX i NOILYNOIS4A LeVd X3QANI uoijesiJojne sues anbiunwwod no pnposdas 3442 au X8V 242108 e ap ajaludoud ej ue d 08 VYLNAd 0L01W1 SNOILVJISIGOW INDINHIIL ILON PUL uo OL 38Vd OVIG ZO LO GL 31VO s 09d HON38 1V9ILdO 39NIHAS _ LI a ii NOLLVNBIS3Q 08 09d ASSY 4410 S 38NIHAS SVL6SVGX OL 08 09d YAS 4410 S 10061 31033N SV9l9vOX 6 X EW JHI A32DS 7 TR L X EW Xd MINIS lt dOL3DNHAS ED 08 09d NOLSId 4410 V070995 S D 08 09d 3231955042 38NIMAS VZ90089 7Z G 39NIHAS 4410 S ONIH O vi90vVd g L ZL 0 DBNIHAS 4410 S DNIH O vos0vVVd z 08 09d M2018 54105 JONIHAS VLEODGD NOLVNDIIS3U H38WRN LUV X30NI cx X Ol 7 UOIJESIJOJNE su
100. IN E E L ery a amp s 3 8 e a K 3 z ao wel a PLT L Threshal s c e few Een fee ass a re ala Ld sut we MP N v 15 v Sli cup 4 er lo favs i GS GAIN 0 cel 2 F e ms gs a o e Olio ere eus o 00 we a Ea RBCGAN I os 5 I ins I Was s Safe OPTICAL ai e BENCHFAN a E E Install new Mother Board and screw the 9 CHC M4x 6 screws Connect all the connectors on the board See Main board connections page 3 A lddfis J9 1 704 HC1T LHNE HET PLT HCT REC 11 BASO WBC Etes Acquisition CPLO gj PIC 16 Dual Stepper e la Motor Control Sampi syringe motor current RAS354B ARRIA z External Reset DE cm BD32 BDARD pe Verti carriage motor current al I MOTORS as BOARD RS232 1 I LEDS gue CPU CPLD CPU CPLD l BOARD E dh bod JTAG EMERGENCY POSIT ASSY VALVES PIC 16 DRAINING 1 22 Thermal amp E DETECT HE I Sensor Control DRAINING 2 VALVES I 7 DETECT 92 3 T COUNTING l i DETECT VALVES 31 35 EN Les TRANSFERT j LS 3 Le i g E DETECT VALVES amp UE W13 19 LEE D fm va Zl Sh Yaa RS23
101. INHJJL 310N PU NOIL VNDIS3Q CYVOd HdV3Md god Svesrvvx 084 099 AZ NIVIA Veecvax z 08d QHVOS YOLOW 494 SV6SrVVX 08d NIYIN ddd Sd9srvvx L XACGNI NOLLVNOIS3Q amp 38IAnN LeVd o OR UorjesiJojne sues anbiunwwo no yinposdas asjya au x gy 24200S ej ajarJdoJd ej ysa uejd a 08 VULNAd gro WEIHCOH Es 6 CO VO SL 31 VG 08d AVAL SNOILLVSH3df1038 SNOILV JISIGOW JNDINHIISL 310N pul NOIL VNUIS 30 H3AO9 LN3OV3H V 00N 2 NS 08d QMVOS NIYN Lz018S 9 AVYL dNOSY 3ONIMAS 610199 S 08d S3ONIMAS OIT MOTJH3AO 610189 Ea HOLON AOTJH3AO dn9 gt zo1g9 God HOLON dOL MO143M3AO dno sLzo189 08d 311109 9V3H MO1SYSAO dN9 7910189 L X3GNI NOILVNOISAG 14Vd uorjesiJojne sues anbiunwwo no JinpoJd J aJja au xgv 4 N0S e ayaisdoud ej ysa ue d Ferrzowovr 08 VMIN3d 92011 SNOL V3IJIQON ANDINHI3L 3LON PUL varios Od 3 INGOW NOIL VNDIS 30 08d YALNdWOD HOS Nv3 svzosvax 9 82 SvoiewX G u3avas Wosr ao add verso AddO 4 ddd wg WV OIM Od Qi VOS NIV add Sveosvvx Z 08d NSIFLNA N3349S 31119 1 ddd SVLLSVVX NOILVNOISAG YSdNNN 14Vd
102. IVD ES LLY LH V0 0V22 SS 0d 1200 HdS 72 0 LIVINOD V8L0V2 viooH8 26 2 43 1102 AZL M6 ULI 821 0 92 1 OW DNILV3H 8210 Q38 92 LH 039 92 Anz NID NI 039 97 JeS2 262 MOL 92 2A 3018 0 DMY 3018 0 DMY JLIHM 92 VLIHSASLYW SNIWIS 493 5 VLC0ZZQ 0LA 0LLS8 720270 OINIW 438 788LHH 1102 DNI1V3H LNIOV3Y Diag 22 XBA387A Reagent heating coil 24 56 ELECTRIC amp ELECTRONIC PRINCIPLES SECTION 3 UOIFEZIVOYING jnouji pajnqiJjSip Jo PaINposday aq jou Aew pue xgy jo Aysadoud ayy si 5 sajaujpuu ug 4 BJEIS EV 3ZiS MIG Z j99uS V68EVSX NMOY 3 8 20 Ag buueo Y 86 01 90 80 ____ O 80 U01101J3S 0 4 p Sseuybnoy aJue J310 VYLN3d 3NI80190N3H SN3 uorjeubisag 02221 9 L 0 XION OWYJHL 3NIVD z v 18 V IW NOILYN9IS3Q EN d3J y UOJJeW LHJ vO2OY22 UOJJ8 7 5 y auner aune V jue g n lg z 2 9 1 LW 1Sf 9 8Hd y najg NOI LVd8ddO 0001 27 72 DMY X 9198 NOILVusdQ0 3 21 XBA389A Hemoglobin photometer Diag 23 XBA389A Hemoglobin photometer 25 56 RAAO22B PENTRA 80 TECHNICAL MANUAL 3 22 XBA393A Upper fan uolyesiJoyne sues anbiunuiuo3 no jinposdas a443 jnad au xgy 242105 ej ap ajalidoud ej uejd sa
103. JL 31ON PUL AGO ALAIdNOD OVA ADNIMAS x NOILVNOISAG 08 09d AGO dWNd WANOVA 39ONIHAS H3HSVM dNNd ALSVWOVA SONIH O IJ L sues anbiunwwo d no yinposday 2442 ynad au Xgy 2421208 e ap ayaisdoud e uejd aj 08 VHLN3d SLO IW SNOILVOIJIOON J0DINH231 310N PUL varuou Us xp sr aava zoom sivc 08d 313 1dlWOO OVA 39NIHAS NOIL VNBIS 3Q L8 GOX HOLIMSOMOIIN HOLIMS VOLO3VO 2 FLV 1d HOLIMSOHOIN YOLOW SSIMGWASSY ANS 2078 133118 veoow3 r ALV1d 3din9 39NIHAS NnnOVA 4 08d ALAIdWOD OVA 3ONIHAS V6r VG0X NOLLVNOIS3Q YSEWNAN LeVd X3cdNI sues anbiunwwod no yinposday ynad au ji X8V 2492108 ap ayaiudoud ej ysa uejd a i 0 V 9L 30Vd Ovid ZO LO OL 31vQ ASSY J NIYAS ALSVM LHOHS NOIL VNBIS3Q 18 GOX HOLIMSOHOIN HOLIMS voLO3vO 9 x ZW OHO MANOS 3lVld HOLIMSOHOIN HO LOIN 09d 313 1d WOO ALSVM 39NIHAS V8v NOX saria assv ans 2018 LN31l S V600 IVA NOILVNOISAG YSdWNN LaVd XSGNI uoljesijojne sues anbiunwwod no JInpoJdaJ 2442 au Xgy 20 e ajalsdoud ej uejd rer O8 VHIN3d LLOTNT SNOILVIISIGOW JNOINHJIL ILON PU d o WNBO EA JDYd DVIG ZO LO 9L 31vO a ASSY amp SalNVHO LNAN NG NOILVNOISAG 08 09d ASSY HNVL 1 901114 J3S8INVHO vgoova
104. NOSTICS e Concerns Replacement of the mixer system e Required tools e Hexagonal keys e Flat screwdriver e Required products None e Intervention time Th Frequency On request e Specific kit or consumables e PC extension cable XEA723AS e Mixer assembly XDA743A Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 Mixer assembly dismantling Turn off the instrument Remove the power supply cable Remove Covers see RAS342B Remove the internal PC See RAS348B and install the XEA723AS extension cable kit 1 1 Top sampler transfer rail dismantling Before dismantling mark with a pen the location of the rail on its support See Diag 1 Rail location page 2 Diag 2Rail screws 1 2 Reagent syringe dismantling Loosen the 4 CHC screws just few turns to free the silent blocks Push the block to the left with the tubes connected See Diag 3 Reager 1 it syringe block page 2 oi Et Diag 3Reagent syringe block 2 6 MIXER REPLACEMENT RAS346B 1 3 Plastic protection Remove the plastic protection located under the mixer by unscrewing the CHC screw See Diag 4 Plastic protection page 3 Diag 4Plastic protection 1 4 Mixer disconnection Follow RAS332B Motor board adjustment procedure to access to the motor board
105. NTION RAS340B PENTRA 80 Calibration 4 44 L 13 3 4 38 L 13 1 L 4 35 L 13 2 FS 271 128 89 16135 Diag 1Calibration window In the event that the result shows an analysis fault or reject the result is not stored An error 14 message advising of rejection is diplayed To discard a result from the statistical calculation click into the Sel column the checked box to remove the result from the statistical calculation If the coefficient of variation is within the limits and the percentage difference between the target and the mean value is less than 20 instrument allows an automatic calibration Press Automatic calculation button to calculate new coefficients then Ok button to confirm and apply new coefficients of calibration Sometimes you may have to use the forced calibration to enter new coefficients when v instrument is very far from its normal range A warning message shall request confirmation to continue validate then perform a control of the instrument 3 Control Run several controls to check and validate the calibration When using DIFFTROL control blood use the floppy disk to update lot parameters Select the Reserved box to link the lot number to QC results for further analyses 3 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE PENTRA 80 Quality Control CONTROL 1 CBC Diag 2Control window 4 4 p traso Optical bench Dismantling amp Replacement Procedure RAS341B
106. No cell in the flowcell Baseline Poorly stained agranular Low cell in the flowcell absorbance Hyper segmented with complex granularity and id absorbance staining Diag 10 Absorbance measurement Results From these measurements a matrix is drawn up with volumes on the X axis and optical transmission on the Y axis The study of the matrix image permits the clear differentiation of 4 out of 5 leukocyte populations As a matter of fact the basophil population is very small compared to the others MONOCYTES The monocytes being cells with large kidney shaped nuclei and a large non granular cytoplasm will neither be scattered nor absorb a large amount of light They will therefore be positioned in the lower part of the optical axis but clearly to the right of the volume axis LYMPHOCYTES The lymphocytes being small with regular shape are positioned in the lower part of both the optical axis and volume axis Normal lymphocyte populations are generally observed with a good volume homogeneity The far left side of the lymphocyte zone should normally be empty but when small lymphocytes are present population may exist in this area The presence of platelet aggregates is detected by a distribution pattern that moves from the origin of the matrix background zone into the lymphocyte zone The NRBCs with their cytoplasmic membranes lysed like the erythrocytes will have their nuclei situated to the far left side of the lymphocyte zone 10
107. On a piece of absorbant paper open the reagent syringe 9 CHC M3x12 screws 2x FX M3x12 screws Replace the pistons See Diag 5 Reagent syringe piston replacement page 4 Use new O rings when performing 6 month maintenance at the same time A The thickness of the washer is matched to each O ring thickness For this reason keep the O ring and the washer together Lyse piston O ring small one had no washer Diag 5 Reagent syringe piston replacement Tightening torques A 9x CHC M3x12 400 mN m 56 8 Ozf in 2x FX M3x12 400 mN m 56 8 Ozf in Reassemble in reverse order Use a dynamometric screwdriver to tighten the syringe 4 8 PISTON KIT REPLACEMENT RAS327B 2 3 Draining syringe 1 piston replacement GBG274A amp GBG260A Switch the instrument off and disconnect power supply cable Remove the cover and the righthand side door to access to Draining syringe 1 See RAS342 Front panel amp covers dismantling procedure for further details Disconnect electrical connectors from the syringe then unscrew the 4 syringe screws just few turns to free silent block See Diag 6 Draining 1 syringe electrical connections page 5 Diag 6Draining 1 syringe electrical connections Put a piece of absorbant paper under the syringe and disconnect all the tubes from the syringe Remove the syringe Drain the syringe pushing the piston nut up and down several times Open the syringe 4xCHC M4x16 screws on o
108. P60 80 MOTOR PIERCING MECHA P60C P80 CABLE T ROOM HARNESS P80 CABLE COAX RBC WBC SENSOR CHAMBERS DRAINING P80 SENSOR HOME RACK TRF MOVE GRAB PCB BARCODE EXTERNAL P60C P80 MOTOR RACK TRANSFER P80 MOTOR RACK LOADING P80 MOTOR RACK LOADING AXIS P80 SECTION 09 Table 1 Spare part list SPARE PARTS LIST XBA478A XBA479A XBA490A XBA492A XBASO7AS XCA166A XCA167A XCA191A XDA481B XDA483B XDA555AS XDAS91AS 592 5 XDAS593AS XDA601BS XDA602C XDA605A XDA610C XDA616AS XDA617AS XDA621A XDA622A XDA623AS XDA625BS XDA626AS XDA655AS XDA657C XDA725A XDA728A XDA729A XDA895AS XDA741AS XDA742AS XDA743A XDA826AS XDA746AS XDA824AS XDA827AS XDA828AS XDA751C XDA752C XDA753C XDA754C XDA755C XDA832AS XDA774A XDA834AS MOTOR CARRIAGE P80 MOTOR BLOCK FOR SAMPL SYR P80 CABLE LMNE FLOWCEL CATHOD WIRE FITTING GROUND FOR REAGENT P80 FAN FOR COMPUTER P80 CHAMBER ISOLATOR LONG CHAMBER ISOLATOR SMALL CHAMBER ISOLATOR VENT P80 C VALVE LIQ 2WAYS NC W O COIL VALVE LIQ WAYS W O COIL TOOL FLOWCELL ADJ KNOB SYRINGE SDIFF ASSY P60 P80 SYRINGE REAGENT ASSY P60 P80 SYRINGE SAMPLING ASSY P60 P80 CHAMBER LMNE FLOWCELL P60 P80 CHAMBER 4 CHAMBERS BLOC P60 P80 CHAMBER DIL TANK ASSY P60 P80 CHAMBER WBC BASO CPTE P60 P80 NEEDLE 190uL SYR P60 SPS P80 NEEDLE 100uL SAMPL SYR P60 P80 O RING VAC WASTE PUMP WASHER O RING REAGENT SYR WASHER REAGENT BLOCK EQUIPED P60 P80 HEATER BLOCK COMPLETE P60 P80 CHAMBE
109. R DIL TANK COVER P60 P80 NEEDLE SAMPL NEEDLE P60C P80 CHAMBER 5 CHAMBERS BLOC P60 80 SAMPLING MECHANISM ASSY P80 C SAMPLING PIERCING BLOCK P80 OPTICAL BENCH COMPLETE PENTRA 80 COVER RIGHT DOOR EQUIPED P80 SWITCH LEFT LOADING EQUIP P80 SWITCH RIGHT LOADING EQUIP P80 MOTOR MIXING COMPLETE P80 MOTOR BLOCK FOR SDIFF SYR P80 MOTOR BLOCK FOR REAG SYR P80 MOTOR BLOC FOR SAMPL SYR P80 SYRINGE WASTE COMPLETE P80 SYRINGE VACUUM COMPLETE P80 VALVE LIQ 12 VALVE ASSY 1 12 VALVE LIQ 7 VALVE ASSY 13 19 VALVE LIQ 4 VALVE ASSY 20 23 VALVE LIQ 7 VALVE ASSY 24 30 VALVE LIQ 5 VALVE ASSY 31 35 SYRINGE SDIFF MOTOR ASSY P80 SYRINGE REAGENT MOTOR ASSY P80 SYRINGE SAMPL MOTOR ASSY P80 5 6 PENTRA 80 TECHNICAL MANUAL 6 6 RAAO22B Table 1 Spare part list XDA790AS XDA791AS XDA792AS XDA793AS XDA794AS XDA795AS XDA796AS XDA797AS XDA798AS XDA803AS XDA804AS XEAO18A XEA286AS XEA311AS XEA410AS XEA486BS XEA488AS XEA616AS XEA663A XEA755A XEA710BS XEA711BS XEA720AS XEA813A XEA722AS XEA723AS XEA736AS SAMPLING RACK TRACTOR ASSY P80 SAMPLING RIGHT RACK LOADER P80 SAMPLING LEFT RACK LOADER P80 BELT RACK LOADING P80 SAMPLING TUBE DETECTION P80 SAMPLING RACK TRACER ASSY P80 SAMPLING RACK LOADING LOCK P80 SAMPLING GRABBER BLOC ASSY P80 CARRIAGE SAMPLING ASSY P80 COVER FRONT LEFT COVER P80 COVER FRONT RIGHT COVER P80 HEAGENT STRAW DILUENT L 360 KIT O RING WASHER P60 P120 P80 KIT FITTINGS KIT
110. RAAO22B Table 2 Tube list 52 280 1 4 1 52 300 3 1682 555 1 1662 440 1 1 52 480 1 152 500 1 1557 550 1 1492 SS 1 152 760 2 1 92 800 1 EAEO08A 2 05 20 1 2 05 65 2 Notched tubing 2 05 70 1 Notched tubing 2 05 80 1 Notched tubing 2 05 100 1 2 05 120 2 2 05 140 1 2 05 140 1 Notched tubing 2205 160 4 2 105 210 1 2 05 280 1 205 300 1 205 370 1 2 05 460 1 2 05 500 1 2 05 550 1 610 1 2105 800 CRISTAL tubing EAEO11A 3 6 2000 1 maxi CRISTAL tubing EAEO28A 4x6 2000 1 oe 1 14 5 2 EAE034A 2 54 20 1 2 54 150 S 2 54 160 1 2057 180 1 2 54 190 1 2 54 225 1 ore ie EAEO47A 0 19 4 1 ic EAEO48A 1 85 10 1 S2x4 Lg 6 tubin Sleevings GAL098A 30 AED x a 9 Tube shielding XBAAOSA 2 6 18 SECTION 2 HYDRAULIC amp PNEUMATIC PRINCIPLES 2 2 Connectors and integrated tubings list The following table shows the connectors and integrated tubings list Length x Quantity per diameter Table 3 Connectors and integrated tubings list Y Connector EABO21A Y Connector EABO26A 225 2 1410 6 Connector EAB033A 1 6 2 T220 6 Connector EAB035A 2 3 8 5 1 02 1330 1 tubing T EAEOO6A 1 30 540 1 tubing 22062 PGO ie 11700 1 tubing 1 EAEO08A 205 7240 1 tubing CRISTAL tubing 11 3x6 2000 1 CRISTAL tubing 28 4x6 2000 1 Hur 1 114 70 1 tubing oe 1 2 54 1225 1 tubing S 1 EAEO47A 0 1
111. RIC amp ELECTRONIC PRINCIPLES 3 17 XAA478A Solenoid board N Diag 19 XAA478A Solenoid board 21 56 sues anbrunuuo no pinposdas jnad au x gy 212005 e syalsdoud ue d Ev Jeu ZZ 922 No WO F o s A Po V EEB 66 21 01 BO 13 WO 4ueans voyea qusus eJ 8 L 2269 00 50 90 13 v lovv3 103P pronez un p inofeu d 774408 4243 2791279090 970229101 OZLWYLN3d 1L3H23Q 011 23130 SN3 uoreubisaQ RAAO22B BUOI S np aaurof 3343 np 3140S 1 9UO2I IS IJAE SASSIA 3343 JUBAIOP V6Z0ZZQ Jna42848p V6LOV V3 SIIOJD SPJ0228J Z 597 2JON V6LOv v3 LAN 8 1 1Q 4102 spJo ey 169089 Vc69089 L 9 NOIL V433dO0 OTLB RZ A 252 ANA 22 202 ANA AY 0 21 L 210 3AIS3Upe 0JNE owsay auien 0002 21 9X47 1V1SI4 291 VSL02V3 971 8301 leu L NOIL V33dO 3 18 XBA322B External waste level detection Diag 20 XBA322B External waste level detection PENTRA 80 TECHNICAL MANUAL 22 56 SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 19 XBA342A Infrared sensor E Mit Z21722 731 1112172274 O wH CN BK RD CAKO31A 1
112. RINTER A PRINTER OUTPUT Material This drawing is the property of ABX and may not be reproduced or distributed without authorization Diag 12 DAD123A DSUB 25pts Flat cable 1000mm 14 56 SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 11 DAD124A DSUB 9pts Flat cable 1000mm wo p 1000 mm PANEL COM2 D e D N M C4 Un cc 5 9 UL HE5 F9 UL CAJ024A CAJ006A REFER TO THE FLAT CABLE MANUFACTURING PROCEDURE RCF013A THE CONNECTOR HE5 MUST BE UL RECOGNISED RTRT2 OR ECBT2 EX ITT CANNON FCI Description hk By Date ECO Rev on DSUB 9 POINTS FLAT CABLE L 1000 _ TENI iste 80 PC COM2 RS232 OUTPUT Standard tolerance Roughness Treatment DADIZEA Sheer S Drw size Ak Scale EFG Din h This TI is the property gt ABX an may not be CAN or ace bare without authorization DIAGNOSTICS Diag 13 DAD124A DSUB 9pts Flat cable 1000mm 15 56 PENTRA 80 TECHNICAL MANUAL RAAO22B 3 12 XAA428C Carriage board Diag 14 XAA428C Carriage board 16 56 SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 13 XAA429A LEDs board Diag 15 XAA429A LEDs board 17 56 RAAO22B EE om JE r aod amp De e fo HEER ast B am tost mg ge J qe DD n Er bui Le 4 i we eq Jl _ Ong
113. SFER SOLENOID DAD119A 8 CBKO44A ese Se J BUREER MOUSE AZERTY OPTION CBKO4SA 6 TUBE MIXER pSTEP MOTOR XBA465A 265 W POWER SUPPLY MODULE DBN006A 1 J45 PAN O 1 lt HOME TUBE MIXER MOTOR XBA432A 4 Er TP42 24V 2 GRABBER STATUS XBA433A h UCTRL 2 1 15V Cx 24V 7 5A 100 240 Vac 3 0 5V 10 24V ON OFF 300 VA PIC 16F872 15V 0 EA2 HEAD LOCKING SOLENOID XBA470A 2 GND 24 A 1 OR DACO12A XBA342A HOME HORIZ CARRIAGE MOTOR _J P110 4 GND HORIZONTAL CARRIAGE XBAL69A 4 gt 5 RB l PIC 16F872 j Di 0 s 20MH n P S o o H PS N I VER Ros gps z POWER SUPPLY I L XBALS9A 2 ie sw Mj ABARRA yk MI A Puan 3077 ms e PWR DRIVER R38 o peTRL XBA464A PIC 16F872 e XBAL59A amp sm zmz E lt XBAGGSA 1 4 n pr um ELECTRICAL SYNOPTIC PWRDRIVER Standard tolerance XBA465A 4 5 4 21 gt A XBAA60A 1 4 NON Ma TEE 2 Treatment DIAGNOSTICS Creation following prototype and 7ANxxxAF h diagram 08707702 feet A j Coating Description Chk By Date Rev N 2 12 Sheet F Drw size Af Scale Dim in millimetres
114. See Diag 4 Right side cover page 3 One of the screws is at the rear of the instrument with a washer E Open Reagent cover lift up then pull it slowly down Diag 4 Right side cover 3 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE Remove it with Reagent cover hinge by unscrewing 3 CHC M4X8 and 2 CHC M3X6 with washers See Diag 5 Reagent cover page 4 Diag 5 Reagent cover Remove Left side cover by unscrewing 6 CHC M4X6 See Diag 6 Left side cover page 4 Diag 6 Left side cover 4 8 FRONT PANEL amp COVERS DISMANTLING RAS342B Remove Left Panel by unscrewing 2 CHC M4X6 at the front and loosening 2 CHC M4X6 at the rear See Diag 7 Left panel page 5 Diag 7 Left panel Remove Loading tray by unscrewing the 2 CHC M4X6 and 1 FHC M4X6 and loosening 1 CHC M4X6 B See Diag 8 Loading tray page 5 To remove loading tray open the 2 Slides then lift the tray from the rear to the front Take care not to damage the switchs Diag 8 Loading tray 5 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE Remove the 4 reagent bottles disconnect tubes from the stoppers Remove bottle recuperation tray Remove the Rear panel by loosening 4 CHC M4X6 See Diag 9 Rear panel page 6 Oe Diag 9 Rear panel Remove Bottle support by loosening 4 CHC M4X6 See Diag 10 Bottle support page 6 Diag 10 Bottle support 6 8
115. Stat mode button page 2 Diag 2 Stat mode button When the following screen appears See Diag 3 Fresh blood tube page 2 put one of the tube and close the tube holder IN Please put a tube of fresh blood in the holder Diag 3 Fresh blood tube When the analysis cycle ends the first result is displayed on the result chart table When the tube holder is open run an other specimen and so until you get a minimum of 5 different results 2 4 LMNE BALANCE ADJUSTMENT RAS337B At least 5 results are necessary to calibrate the LMNE balance Select results to be involved in the statistical calculation by clicking the checked box in Sel column See Diag 4 Old coef page 3 To discard a result from the statistical calculation click the checked box again to remove the mark PENTRA 80 Others Technician LMNE Calibration Cycles Calibration Coefficients Blank Values Burn in e Sel Op TestDate GBBaso GBLmne v Techni04 18 2002 14 36 20 8 4 8 7 Automatic Calibration v Techni 04 18 2002 14 37 06 4 7 4 2 Techni 04 18 2002 14 39 03 4 7 4 1 Techni 04 18 2002 14 39 49 10 0 8 8 TechniO4 18 2002 14 43 03 10 0 9 2 GBBaso GBLmne Current Coef 1 0 79 New Caef 1 0 85 Mean 7 6 7 0 N Version V0 33 Diag 4 Old coef Click on auto calibration button See Diag 4 Old coef 3 to apply new calibration coefficient When the fo
116. TECHNICAL MANUAL RAAO22B 2 7 1 Maintenance 1 1 Introduction Customer maintenance has to be carried out according to the recommended frequency chart A table and after having attended an ABX approved customer training course The system warranty may be affected if damage occurs after a non trained technician intervenes or if replaced spare parts and consumables do not come from an ABX approved origin 1 2 Daily customer maintenance No special adjustment or maintenance has to be done on your equipment if the recommended startup and shutdown procedures are explicitly respected See User s manual for daily rinsing and cleaning of the system 1 3 Weekly customer maintenance An overall check for cleanliness of the system is recommended every week All traces of blood or reagent have to be wiped off as soon as possible using a piece of cloth and distilled water Never use solvent or abrasive cleaning to clean the system 1 4 Maintenance table Pentra 80 maintenance is scheduled on 3 different maintenance kits The maintenance schedule depends on the number of cycles per day follow the chart table to know kit part number to be used Table 1 Maintenance table Kit P n Needle O ring X Heagent syringe O ring X X X X X X X X dn rat Counting syrin X X X X X X X X RAS325 Sampling syringe X X X X X X SDIFF syringe X X X X X X Draining syringe X X X X X X XEA486CS Optical bench lamp X X X X X X Procedure LMNE flowce
117. Thermic panel 1 5 Waste Waste Control Hemove Waste Detector from waste then move detector from the bottom up and check that Waste Control status becomes red See Waste control page 5 Diag 11 Waste control 5 10 PENTRA 80 TECHNICAL MANUAL SENSORS CHECK AND ADJUSTMENT PROCEDURE 2 Sensors Adjustment 2 1 Loading area Stop Rack Loading Sensor Left reference XDA741AS Stop Rack Loading Sensor Right references XDA742AS Adjustment of this two sensors is the same This sensors are delivered on bracket and are 14 prestressed This adjustment is factory made Loosen the CHC M4x12 screws to adjust switch assy switch bracket CHC M2x10 screws flat nut Diag 12 Stop rack loading sensors adjustment The axle of the switch wheel does not hang out of the front plate in order to not damage switch when rack is moving back See Switch wheel position page 6 Axles Front plate Diag 13 Switch wheel position If the switch as been moved on bracket loosen the two CHC M2x10 screws and adjust it on bracket to have the detection after 0 5 mm of moving See Bracket switch adjustment page 7 Plug a voltmeter in ohmeter positon between the two external pin to check detection Reference switch alone CAE019A 6 10 PROCEDURE RAS356B Bracket mechanical Stop position Detection position Bracket Diag 14 Bracket switch adjustment 2 2 Sampler Transfer Rail Tube Height
118. Value o00 PENTRA 80 A Press Enterto Continue Drain 2 Syringe Motor Version V1 3 0 VACUUM_DRAIN_SYR_1 03 16 2004 09 30 03 Diag 3 Validate Disconnect the Barflex and connect the tube back on the draining syringe 1 zu If vacuum is not stable or too low check vacuum circuit tubings valves syringe or O ring and repair if necessary 1 2 Draining syringe 2 Disconnect the tube on the draining syringe 2 inlet 1 and replace it by the Barflex tube See Diag 4 Draining syringe 2 page 3 Diag 4 Draining syringe 2 Enter Menu Service Menu Technician Gains Vacuum control See Diag 2 Vacuum control page 2 Press Draining syringe 2 Check vacuum on the Barflex vacuum must be stable during 30 secondes Press OK button See Diag 3 Validate page 3 Disconnect the Barflex and connect the tube back on the draining syringe 2 3 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 2 Counting syringe vacuum check and adjustment 2 1 Counting syringe vacuum check Remove cover See RAS342 procedure Disconnect the lowest tube inlet 4 on the counting syringe and replace it by the barflex tube See Diag 5 Counting syringe page 4 ESS _ Diag 5 Counting syringe Enter Menu Service Menu Technician Gains Vacuum control See Diag 2 Vacuum control page 2 Press Counting syringe Check vacuum on the Barflex while the following screen is displayed See D
119. WHITE CCA043A 6 EHR amp CCAO4LA EHR 6 400 Ref JST EHR 4 4 COLLIERS 2 4x92 CCAOLSA DBH001A Contacts SEH 001T P0 6 Ref JST EHR 6 CCA045A Contacts SEH 001T P tion p perm Ba _ Modification pales Dule OH Res D signation TORON MOTEUR lg 400 JST Tol rance Generale CERTES Mati re Traitement Revetement N XBA463A Folio Z Format A4 Ech 4 Dim en millim tres Ce plan est la propri t de la societe ABX il ne peut tre reproduit ou communique sans autorisation Diag 38 XBA463A Motor Jst 400mm 40 56 SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 37 XBA464A Motor Jst 500mm COA043A EHR 4 Ref JST EHR 4 CA045A Contacts SEH 001T PO 6 D g ul p CCA044A EHR 6 500 5 COLLIERS 2 4x92 DBHOOTA Ref JST EHR 6 CCA045A Contacts SEH 001T P Cr ation CT DB 06 10 00 5304 Modification Designation TORON MOTEUR lg 500 JST Tolerance Generale Etat de Surf Matiere Rev tement f Rev tement N XBA464A Folio Z Format A4 Ce plan est la propri t de la soci t ABX il ne peut tre reproduit ou communiqu sans autorisation Diag 39 XBA464A Motor Jst 500mm 41 56 PENTRA 80 TECHNICAL MANUAL RAAO22B 3 38 XBA465A Motor Jst 700mm 24 WHITE 2 2 WHITE M 5 24 WHITE
120. X0057 HAX0058 LAD002BS 9 RAA022A RAB108E RACO57C XAAA29AS XAA456BS XAA458AS XAA459BS XAA478AS XAAS47A XAA548A XAA509CS XAA510CS XAA511AS XBA322B XBA342A XBA389A XBA411A XBA425A XBA398B XBA431A XBA432A XBAA453A XBA474A XBA475A XBA476A CUP OVERFLOW TOP MOTOR PCB P80 CUP OVERFLOW MOTOR BOARD P80 SAMPLING OPT TUBE HOLDER P80 RACK 10 TUBES 13x82 P80 STICKER VALVES JOUCO 1 10 STICKER VALVES JOUCO 11 20 STICKER VALVES JOUCO 21 30 STICKER VALVES JOUCO 31 40 STICKER COVER LABEL PENTRA 80 STICKER COVER LABEL ABX STICKER PC CONNECTIONS P80 S TICKER RACK LABEL A 01 20 lt S TICKER RACK LABEL B 01 20 lt S TICKER RACK LABEL A 21 40 lt STICKER RACK LABEL B 21 40 lt S TICKER RACK LABEL A 41 99 lt S TICKER RACK LABEL B 41 99 lt TOOL LATEX RBC PLA TOOL TORX KEY T10 MANUAL TECHNICAL P80 MANUAL USER S P80 GB MANUAL USER S P80 FR PCB LED BOARD FOR COVER P60 80 PCB MAIN BOARD P80 PCB PREAMP LMNE P80 PCB MOTOR BOARD P80 PCB SOLENOID RACK TRANSFER P80 PRINTER OKI B4200 220V PRINTER OKI B4200 110V PCB MAIN BOARD PC W O RAM P80 PCB IDE HARD DISK LOADED P80 PCB TACTILE PANEL FUTJISU P80 SENSOR WASTE DETECTION P120 80 SENSOR CARRIAGE MOVE P80 PHOTOMETER HB CPTE P60 P80 FAN MAIN FAN 24V P60 P80 SENSOR SAMP NEEDLE MOVE P60 80 FITTING GROUND FLOWCELL
121. X3GNI uorjesiJo4ne sues anbiunwwod no yinposdas asya ynad au X8V 205 e ayaiudoud ej ysa uejd a rwa 09 WHLNAd POT SNOIL V2IJIQON 300INH231 310N PUL zoor sva m ks 08d 82 Q MVYLS LN3OV3MH NOIL VNDIS 30 08d 8 MVHLS IN3OV3H VSr SgS EN NOILVNOIS3Q LSVd X3GNI yoljyesivoyne sues anbiunwwod no yinposdas 2442 au J X8V 1 1205 e ap ayaisdoud ej ysa uejd a g sirsewewra 09 VWHINAd LSOIW SNOILV JISIGOW JNDINHIJL 310N PUL zc eg V zv ava zonomz ava 38V1 MOV H3MOILS NOIL VNDIS 30 66 OL LV HdAL 1H8V 1 MOV LEOOXVH Or OL Lc HdAL 138V 1 MOVM OCOOXVH EEK 0c OL L SAdAL 138V 1 MOV 6c00XVH 66 Lv ga TH8V1MOVM H3MOILS 8S00XVH 66 Lv TH8 V1 MOVE H3MOILS LSOOXVH
122. and as such will increase the LIC count Small blasts will be found between the normal lymphocytes and monocytes Platelets and debris from erythrocyte lysis represent the background noise population located in the lower left area of the matrix Most of the population partition thresholds are fixed and give the limits of the morphological normality of leukocytes Changes in the morphology of a population will be expressed on the matrix by a shifting of the corresponding population A Blast alarm is generated from increased counts within the LIC area this is correlated with Blast detection on the Basophil curve Large lymphocytes are detected in the ALY Atypical Lymphocytes zone where reactive lymphoid forms stimulated lymphocytes and plasmocytes are also to be found 11 12 PENTRA 80 TECHNICAL MANUAL RAAO22B 12 12 MOSS Technology pentra 80 Software release 1 Service software 5 2 Te SEN IM I t 5 2 IEEE TE 5 3 1 3 Concentrated Ole od erect 5 3 1 4 Autoclean cccccccceccececcecccceccececcecesceseecescecesceceseeseesans 5 3 1 5 Technician Menu unies L L uuu uu 5 3 2 Windows EXPIOrer ACCESS nu ee 5 4 3 Software releases 5 4 3 1 Software modification 5 4 DIAGNOSTICS PENTRA 80 TECHNICAL MANUAL 1 Service software overview 1 1 Super User Menu 1 1 1 Mechanical 1 1 2 Hydraulic
123. and erythrocytes that are resistant to lysis may interfere with an accurate LYM count Limitations listed for the WBC count pertain to the LYM and counts as well MON mononuclear cell count absolute MON Mononuclear percentage e The mononuclear cell count absolute is derived from the WBC count The presence of large lymphocytes atypical lymphocytes blasts and an excessive number of basophils may interfere with an accurate monocyte count e Limitations listed for the WBC count pertain to the MON and counts as well NEU neutrophil count absolute NEU Neutrophil percentage e The neutrophils cell count is derived from the WBC cell count The excessive presence of eosinophils metamyelocytes myelocytes promyelocytes blasts and plasma cells may interfere with an accurate neutrophils count EOS Eosinophil cell count absolute EOS Eosinophil percentage e The eosinophil cell count is derived from the WBC cell count The presence of abnormal granules degranulated areas toxic granules may interfere with the eosinophil counting BAS Basophil cell count absolute BAS Basophil percentage e The Basophil cell count is derived from the WBC cell count p traso Hydraulic amp Pneumatic principles 1 Hydropneumatic connections 2 2 Instrument CU WS u U Uus uuu usssasssassussapaq 2 5 TR a sss sasa 2 5 2 2 Connectors and integrated tubings list
124. anel amp Covers dismantling for further details Remove the white vertical plastic mother board protection Loosen the 2 diluent reservoir fixation screws to free the diluent reservoir See Diag 11 Diluent reservoir dismantling page 8 Diag 11Diluent reservoir dismantling Open the diluent reservoir without disconnecting its tubing 4 screws on the top Locate the new joint and washer against the reservoir top See Diag 12 Diluent reservoir assembling page 8 Diag 1 2Diluent reservoir assembling Locate the top part of the reservoir to have the both tube turned in front of the diluent reservoir Top screws tightening torque 120 m Nm 17 Ozf in Screw the 4 diluent reservoir screws according to the tightening torque above If the tube under the reservoir body has been disconnected connect it before installing back the diluent reservoir in its location Install the diluent reservoir in its location and block the screws 8 12 YEARLY MAINTENANCE RAS326B 9 Draining chambers O ring replacement 4xFAA066A Make sure the chambers are drained if not run a Services Super User Menu Hydraulic Unprime Cycle All command to drain the chambers Put a piece of absorbant paper or cloth under the chambers assembly Turn gently the chamber s bottom between 2 fingers See Diag 13 Draining chamber dismantling page 9 Diag 13Draining chamber dismantling Replace the O ring See Diag 14 Draining cha
125. ations must be applied in all the operations 1 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 How to access Main board e Remove the righthand side cover of the instrument e Open the righthand side front cover e Remove the thermal panel e Unscrew the 9 screws from the righthand side cover and carefully remove the cover e Unscrew the 2 screws on the top of the frame from the inside protection and remove it 2 8 MAIN BOARD ADJUSTMENT RAS331B 2 Main board general view LMNE PREAMPLI BOARD RBC PLT CIS BASO WBC CIS HGB MEASURE D i ius J 09 e n e I Die ve sw 0 m HGB GAIN gt L em n L 5 N a gt S F E x 1 lt un En 3 MEN a PLT L Threshold D GAIN s s 5 m m 0 ma mia LE L Me Same 9 suc E 9 wir owe s d dons dc 2 15 GAIN ws I mz rEg o _ IET Threshold fs GND GND wa gre Md w 05 MP gt RBCGAN a I en 7e S SIZE OPTICAL F e BENCH FAN Ex fo usa HCTI LMNE HCN PLT HC11 RBC a E Acquisition CPLD DL Ulla PIC 16 m Dual Stepper 9 Motor Control Sampl syringe matar current CARRIAGE iy External Reset BD32 BOARD T I
126. bad position Barcode internal connection problem System stop due to synchroni zation problem Analyzer already in cycle Run an Initialization Run an Initialization Run an Initialization Run an Autoclean Run an Initialization Run an Initialization Run an Initialization Run an Initialization Run an Initialization Run an Initialization Run an Initialization Run an Initialization Run an Initialization Run an Initialization Run an Initialization Run an Initialization Run an Initialization Run an Autoclean Run an Initialization Run an Autoclean Run an Initialization p ntraso Maintenance NASA 7 2 TREO Lu uu un nuan 7 2 1 2 Daily customer maintenance 7 2 1 3 Weekly customer 7 2 1 4 Maintenance table 7 2 2 Maintenance kits c 7 3 2 1 6 month maintenance kit 7 3 2 2 Yearly maintenance kit 7 3 2 3 Pistons maintenance entr mure 7 3 ZA FURO KI ee 7 4 7 4 3 Maintenance procedures 7 4 3 1 Required tools and products 7 4 3 2 Procedures chart table 7 5 3 3 Training department procedure list 7 7 DIAGNOSTICS PENTRA 80
127. board the Hgb blank voltage to 4 7V 0 02V 3 7 Startup Run a Startup cycle 3 8 Repeatability Check repeatablility running 10 analyses with on the same blood sample From menu Quality Assurance Within Run run 10 consecutive analyses 3 9 Calibration Perform a calibration of the instrument Select and enter Calibrator lot parameters If necessary force calibration to enter the new coefficients 3 10 Control Run several controls to validate the calibration When using DIFFTROL control blood use the floppy disk to update lot parameters Select the Reserved box to link the lot number to QC results for further analyses 3 11 Config save and print After installation is completed archive and print instrument setup by following procedure RAS357 Save and restore settings p iitra 80 6 month maintenance RAS325B DIAGNOSTICS e Concerns e Rinsing block e Reagent syringe e Draining syringe 1 e Draining syringe 2 e Counting syringe e Instrument amp Filter cleaning e Required tools e Hexagonal Keys e Dynamometric screw drivers A302 A301 A300 e Cutting pliers e Flat screw driver e Philips screw drivers e Torx keys e Required products Minoclair e Intervention time 2h e Frequency See maintenance table e Specific kit or consumables 6 month maintenance kit XEA710BS Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all
128. ccess to the rinsing block Remove the 2 rinsing block screws See Diag 1 Rinsing block dismantling page 2 Diag 1Rinsing block dismantling Lift the locker See Diag 2 Lift locker page 3 to free the probe and remove the rinsing block 2 8 PISTON KIT REPLACEMENT RAS327B Diag 2Lift locker Replace the percutor See Diag 3 Rinsing block unit page 3 GBG169A Diag 3Rinsing block unit Use new O rings when performing 6 month maintenance at the same time Change the needle XDA655DS when performing Yearly maintenance at the same time Do not tight too much the rinsing block screws Torque 120 mN m N 17 Ozf in Reassemble in reverse order 2 2 Reagent syringe pistons replacement GBC031A amp 4xGBC030A Switch the instrument off and disconnect power supply cable Remove the cover and the lefthand side panel to access to the reagent syringe See RAS342 Front panel amp Covers dismantling procedure for further details Disconnect the following tubes See Diag 4 Reagent syringe dismantling page 4 e Valve 7 Inlet 3 e Valve 8 Inlet 3 e Valve 9 Inlet 3 e Valve 10 Inlet 3 e Valve 12 Inlet 3 3 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE Diag 4Reagent syringe dismantling Unscrew the 2 fixation screws and remove carefully the reagent syringe See Diag 4 Reagent syringe dismantling page 4 Push several times the piston over the waste container to drain the syringes
129. cessary when heating block has been changed for example perform the following point of this procedure 2 2 Thermostatic compartment temperature adjustment Check and eventually change the CIN value See Main Board Thermostatic compartment CTN value page 4 il T ur X LE P nE Diag 5Main Board Thermostatic compartment CTN value The Thermostatic compartment CTN value is located on the CTN wire close to the main board connected on J33 Be sure to read the correct value as the sticker may be up or down Adjust as described for the control the temperature by the mean of the Temperature recorded value in the Thermic adjustment window See Thermic adjustment window page 2 Temperature recorded value in the Thermostatic compartment area of the window must be rounded ex 34 35 36 e f Temperature recorded is increased reagents preheating temperature increases 4 6 TEMPERATURE ADJUSTMENT RAS333B e If Temperature recorded is decreased reagents preheating temperature decreases Make a Thermostatic compartment temperature check after the adjustment as descibed at the previously in this procedure If required change the Temperature recorded value and check temperature again until you get an acceptable temperature 5 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 6 6 p ntra 80 Vacuum Adjustment Procedure RAS334B DIAGNOSTICS e Concerns Counting and draining 1 amp
130. circuit M _ o m i ar a sivo Han Ta Ls niv Ys z L DIC ae H 22 ABX DJLUENT pan ee eee ee SECTION 2 HYDRAULIC amp PNEUMATIC PRINCIPLES 4 9 LMNE injection circuit lt DILUENT WASTE gt s Li ma fi di E E s na rel iai sd T T 12 ah LE namen x v 2 4 inant s 91503 MAY LIN 6 di B LEY SMS 17 18 PENTRA 80 TECHNICAL MANUAL RAAO22B 5 Pneumatic diagram See pneumatic diagram on next page 18 18 2 06 800 2 06 800 1 52 760 TRANSLATION Inch to mm Int diam 152 800 Js 152 200 mE S VENT TUBING 1 j 2 06 800 2 CRISTAL TUBE TYGON TUBE S SILICON TUBE 0 V ISOLATOR ISOLATOR a ns 130 15 SAMPLE SYRINGE DRAIN BUBBLING ISOLATOR a lt 1 52 335 1 52 90 x L i DRAIN SYRINGE lt g os he 2 06 610 d 1 52 500 ees 1 52 120 LMNE TRANSFERT BUBBLING DRAIN COUNT WBC RBC 13 PROBE WIPE WBC BUBBLING DILUENT FILL DRAIN RESERVOIR FILLING LMNE RINSE VENT 15 NOTCHED TUBING z
131. ck on Loading Tray and adjust it on the center to have the same left and right gap See Diag 5 Rack position page 4 Diag 5 Rack position Press Check Transfer Home button See Diag 1 Sampler adjustment menu page 2 When carriage is coming under rack gently push finger up to check position use screwdriver or pencil Carriage Finger must be adjusted in hole center See Diag 6 Carriage position page 5 4 8 AUTOMATIC SAMPLER RAS347B Hole RACK CARRIAGE m FINGER Diag 6 Carriage position At the end of Transfer Carriage motion Carriage motor is no more supplied Make sure it does EP not move when you push finger up Adjust Transfer Home value to center Carriage Finger into rack hole If finger is on the right increase Transfer Home value If finger is on the left decrease Transfer Home value Press Check Transfer Home button to check position Repeat the procedure until you get the correct adjustment 3 2 Stop Transfer Stop Transfer value represent the number of steps after Stop Transfer switch detection 9 After Transfer motion you have only 4 seconds to chek position before rack ejection Put the rack on Loading Tray Press Check Stop Transfer button See Diag 1 Sampler adjustment menu page 2 At the end of transfer motion before ejection make sure there is a bit of play around 0 5 mm between mechanical stop Ejection Tray side and rack See
132. cking Working conditions Instrument installation First start of the instrument Dismantling of all instrument s panels and covers Rinsing block Reagent syringe Draining syringe 1 amp 2 Counting syringe Filter Sampling syringe O ring 5DIFF syringe O ring Draining chambers O ring Optical bench lamp and coaxe Diluent reservoir Counting chambers oampling probe amp guide replacement Draining syringe 1 amp 2 Coun ting syringe Reagent syringe Draining syringe vacuum check Counting syringe vacuum check Counting syringe vacuum adjustment Bubbling adjustment Heagent heating coil and thermostatic compartment temperature adjustment Chambers assembly position check and adjustment Check and adjustment of the MDSS Hgb blank adjustment RBC PIt gain adjustment WBC Baso gain adjustment Drain sensors adjustment Motor current adjustment Thresholds adjustment LMNE flowcell position adjustment MNE balance calibration and forced calibration Heplacement of the mixer Loading mechanism check and adjustment Mixer mechanism check and adjustment Transfer mechanism check and adjustment Check and adjustment of the instrument s switches Adjustment of internal barcode Tube holder assembly replacement Tube holder adjustments Needle adjustment Compatible tube list Motor board currents check and adjustment Heplacement of the computer Heplacement of computer s hard disk Heplacement and set
133. conditions 2 1 Environment The Pentra 80 should be operated in an indoor location only Operation at an altitude over 3000 meters 9800 feet is not recommended Instrument is designed to be safe for transient voltages according to INSTALLATION CATEGORY Il and POLLUTION DEGREE 2 Please ask your ABX Diagnostics representative service center for any information about the operating location when it does not comply with the recommended specifications 2 2 Location The Pentra 80 should be placed on a clean and leveled table or work station Please note that the Pentra 80 printer and reagents weigh approximately 40 kilograms 88 lbs Avoid exposure to sunlight Proper ventilation requires that a space of at least 20 cm 8 inches must be left behind the instrument 2 3 Grounding Proper grounding is required Check that the wall ground earth plug is correctly connected to the laboratory grounding electricity installation If there is no ground then use a ground stake Current electricity Standards must be applied 2 4 Humidity and temperature conditions The Pentra 80 must function between 16 to 34 C 61 to 93 F Maximum relative humidity 80 for temperatures up to 31 C 88 F decreasing linearly to 50 relative humidity at 40 C 104 F If it is kept at a temperature of less than 10 C 50 F the instrument should be allowed to sit for an hour at the correct room temperature before use 2 5 Electromagnetic environment check
134. ction to ensure correct rack position Put a rack on Loading Tray then press Check Stop Rack button See Diag 1 Sampler adjustment menu page 2 When motion is ended make sure there is a bit of play between Slides rack and Front Plate Press rack on the Front Plate then check play 1 between rack and Slides It must be around 0 5 mm See Diag 3 Rack and Front Plate play page 3 PRESS RACK front plate Diag 3 Rack and Front Plate play 3 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE Make sure that motor does not loose steps noise when Slides come against the rack because it can return a little bit if motion is too big If play 1 is too big increase Stop Rack Loading value If play 1 is too small decrease Stop Rack Loading value Repeat the procedure until you get the correct adjustment 2 Mixer mechanism check amp adjustment Mixer adjustments are factory made and parameters are writing on a label on mixer body They must not be modify but if necessary follow Mixer replacement procedure RAS346 3 Transfer mechanism check amp adjustment Transfer home value represents the offset of Transfer Carriage Follow the procedure in this Z order because First Transfer and Rack Ejection are depending on Transfer Home 3 1 Transfer Home On a rack side mark off the hole center position with a pencil See Diag 4 Rack Mark page 4 Diag 4 Rack Mark Put the ra
135. ctly plugged on Hard Disk because 4 pin are free See Hard disk connector page 5 and that blue band on FPC cable Floppy disk reader connector is in the correct position See Floppy disk reader connection page 3 Diag 8 Hard disk connector Restart system and restore configuration See RAS357 5 6 PENTRA 80 TECHNICAL MANUAL PC HARD DISK REPLACEMENT PROCEDURE 6 6 p ntra 80 PC Mother Board Replacement Procedure RAS350B DIAGNOSTICS e Concerns PC Mother board replacement e Required tools e Allen Keys e Flat Screwdriver e Required products None e Intervention time 1 hour e Frequency On request e Specific kit or consumables Mother Board kit XAA509BS Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations Remove internal PC See RAS348 1 6 PENTRA 80 TECHNICAL MANUAL PC MOTHER BOARD REPLACEMENT PROCEDURE Unscrew the 6 CHC M4x6 screws to remove Internal PC from the cover Unscrew the 4 CHC M3x6 and the 4 CHC 4 6 screws See Cover plate fan page 2 Do not remove Left Angle Plate A in order to keep the PC Cover adjustment A Diag 1 Cover plate fan Disconnect fan and remove cover plate Unscrew the 4 CHC M3x6 screws M3 Washers and lift Hard disk CD ROM Drive Floppy Reader assy See Hd assy screws page 2 Diag 2 Hd assy screw
136. cts rinse to probe rinse block or WBC BASO bath i inn LV11 Diluent output control Routes diluent to probe rinse block or heating coil Designation PENTRA 80 LV13 Flow cell rinse Routes diluent from flow cell and LMNE syringe to the count syringe HYDROPNEUMATIC DIAGRAM LV15 WBC and RBC PLT count valve Opens vacuum count line for WBC and RBC bath Standard tol R h LV17 Waste syringe 2 vent Opens waste vent through the rinse chamber andar oterance ouqnness LV19 Diluent reservoir fill Opens reservoir vent and fill it with drain syringe 2 Material LV24 Waste syringe 1 vent Opens waste vent through the rinse chamber Be OEE Nt Selec n Te ee tia The tubing 102 250 was 240 T2 3 LMNE syringe 5 and tubing 114 85 was 1 02 T amp 1 LMNE syringe 3 02 1704 988 B Lireatment DIAGNOSTICS LV28 Flow cell drain Opens path from flow cell to DIFF bath for drain Creation according prototype and NMLOXXAS q diagram 07 08 02 861 A Coating hkl By Date ECR Rev N NMLO12A Sheet S This drawing is the property of ABX and may not be reproduced or distributed without authorization p ritra 80 Electric amp Electronic principles deel AAO OG Gl ERR 3 3 1 1 Main board general view 3 3 2 DOC uu uuu uu a ne DU TUNE 3 4 2 1 Motor board general view ss 3 4 3 Connectors and other boards 3
137. cytes and Monocytes and Neutrophils and Eosinophils and Basophils and Large Immature Cell and Atypical Lymphocytes and Red Blood Cell Hemoglobin Concentration Hematocrit Mean Corpuscular Volume Mean Corpuscular Hemoglobin Mean Corpuscular Hemoglobin Concentration Red Distribution Width Platelets Platelets Distribution Width Mean Platelet Volume Plateletcrit 1 2 Throughput Analyses e 80 samples per hour 1 3 Tube identification e By mean of Keyboard internal and external Barcode 2 10 SECTION 1 1 4 Reagents e ABX DILUENT 20 Litres e ABX CLEANER 1 Litre Integrated e ABX EOSINOFIX 1 Litre Integrated e ABX BASOLYSE 1 Litre Integrated e ABX LYSE 0 4 Litre Integrated 1 5 Internal Computer e Color LCD touch screen 12 inches e Industrial PC board n Windows NT 4 0 for Pentra 80 Windows XP for Pentra XL80 e Processor frequency Celeron 433 MHz e Memory capacity 128 Mo for Pentra 80 256 Mo for Pentra XL80 e Hard drive 20 Go e Floppy disck e CD ROM drive e RS 232C e USB Ethernet for Pentra XL80 e Keyboard e Mouse 1 6 Measurements and computation e Impedance for Wbco Plt Rbc Baso e Photometry for Hob e Impedance and light scattering for Lym Mon Neu Eos Aly and Lic e Computation from stored data that was dir
138. d VHO3WONIOM3lId HOLOW vilwax 08d AVHL MOV dND woo NOILVNOISSAG S38lINFN LYVd X3GNI Y O vA gt W Q lt uoijesiJojne sues nbiunuuuuo3 no yiNposdas JJ jnad au J Xgy 8436005 e ep ayaiudosd ej ysa uejd aj 08 Vd LN3d LSO IN T NOILOALAC JgNL ONMdNVS NOIL VNDIS3Q SNOILV JI3IQOM AINDINHIISL 310N pul NOLLO313Q 39NnL ONI TdINVS SVr6 VaXxX E 23 NOILISOd SNA SVS6ZVO0X L NOILVNOISAQ YSdAWNN LYVd XAQANI DESIGNATION a e D Z X lt a gt lt lt COVER CLOSING MAGNET P80 FAJO07A DESIGNATION COVER CLOSING MAGNET P80 A ind NOTE TECHNIQUE A Creation PENTRA 80 Ce plan est la propri t de la soci t ABX il ne peut tre reproduit ou communiqu sans autorisation MODIFICA TIONS uorjesiJojne sues anbiunwwo no yinpoudas asya au ji Xgy 3430S ej ap ayaisdoud ej ysa uejd a 09 VHLINZId prow SNOIL V2IJIQON J0DINH231 310N pu varios gt 5 K K eee V 30Vd 20 60 12 31VQ NOILVNUIS 10 GOYHSNd 9373 MOVH AOVINNVO c900189 NOILOJ 3 MOVMW 08d AVYL NOILOSra MOVE dno 8900149 NOILVNOISSAG dIANNN 1 uorjesiJojne sues anbiunwwod yinposdas 2143 au J Xgy 22008 ej ep ayaiudoud ej uejd 08 Vd LN3d ZVO IW w S zoos sva 08d SO4VOY 08 VULNAd VHIHOH SNOILVJISIGOW JNO
139. d v9 2 13 1Q33N 1138 VL LONE E NOILLVNOIS3Q e38INTN LHYYd X3GNI uojjesiJojne sues aNDiunWWOd no fiINposday 2442 ynad au XgV 2 ayalsdoud ej jsa aj asss 09 801 11 SNOILVIISIGOW JNOINHJJL 31ON PU gt lt LE 39Vd 11V0 KE 08d ASSV 4344VH9 ONIIdNVS NOILVNOISAG 08d ASSY 9019 4294V49 ONIIdNIVS SV46 VOX FS NOILVNOISAG e 38INTN 1 L sues anbiunwwo no yinposdas au Xgy 242005 ej ayalsdoud ej uejd aj pur _ 1 NOIL VNDIS 30 08d 313 1dINOO ONIXIN HOLOW Vevlvdx NOILVNOISAC YSdNAN LYVd XAQANI 08d SALAIdNOD ONIXIN HOLOW uoljesisoyne sues anbiunwwo no jinpoJdaJ au JI xgv 3481205 eJ ayaisdoud ej uejd a 08 VH IN3d LC01W1 SNOILV JISIGOW JNDINHD3L 310N PUL 08d HOOTONIAVOTHOVA ONTIANVS NOIL VNBIS3Q 08d MOOT DNIGVOT MOVY ONIIdWNVS SV962VOIx NOILVNOISAC YSEWNNN LHYYd X43GNI uoijesiJojne sues anbiunwwo no yinposdas asja au jl Xgy 34 N0S e ayaisdoud ej jsa ued nova 09 WHINGd 0 011 SNOIL VOIJIQOH INDINHI3L 31ON LICE RTE un lt 08d LN3INN3OWHVHO SNS NOIL VNDIS3Q 08d SNIQVO1MOVM 1138 svewox 2 MOVY 14371 ON IANYS Swewax 9 YAAVOT MOVY LHOIY ENFTdIAVS svieivax 3201 SNIQVO1 MOVE ONITdIAVS swevax 08d 909
140. de sticker in position 1 and 2 to check the correct barcode reading operation Enter Menu Service Technician Menu Gains Sampler Adjustment Put the rack on Loading Tray Press Barcode Reader Adjustment button Rack is moving in front of Barcode Reader and cycle starts At the end of cycle Rack label reading zone must be within bargraph zone See Diag 1 Barcode screen page 2 Tube 2 reading zone Rack label reading zone Tube 1 reading zone Sampler Utilities Bargraph zone a om Diag 1 Barcode screen A Tube reading zone depens on label width and label condition If there is reflections reading zone may be in several parts INTERNAL BARCODE ADJUSTMENT RAS343B 2 Barcode reader adjustment If Rack label reading zone is not within bargraph zone free the Barcode Reader Support Plate by loosening the two M3x6 CHC screws See Diag 2 Barcode reader page 3 then move it to adjust reading position When you are in front of the system If Rack Label Reading Zone is on the right pull barcode reader on the EjectionTray side If Rack Label Reading Zone is on the left push barcode reader on the LoadingTray side Press Barcode Reader Adjustment button and check Rack Label Reading Zone position Repeat this procedure until you get the correct adjustment Diag 2 Barcode reader 3 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 3 Barcode configuration 3 1 Typology and Ckeck D
141. decreases Make a Reagent heating coil check after the adjustment as descibed at the beginning of the procedure If required change the Temperature recorded value in the Reagent heating area of the window and check temperature again until you get an acceptable temperature 2 Thermostatic compartment temperature check and adjustment 2 1 Thermostatic compartment temperature check Start the instrument Enter menu Service Technician Measurement Gain The Thermic adjustment window is displayed See Thermic adjustment window page 2 3 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE This control must be made with the thermal door closed and under stabilised thermic conditions At least 5 minutes after startup Open the thermal door on the righthand side of the instrument Put the thermometer sensor in the CTN sensor See Thermostatic compartment thermometer s sensor installation page 4 Diag 4Thermostatic compartment thermometer s sensor installation After thermometer probe is installed close the thermal door Enter in Run Cycles Number field in the Thermostatic compartment area of the window 5 cycles to run Press Check Thermic in the Thermostatic compartment area of the window button to start control At every bip your ear from the machine check the temperature is around 35 C 1 C Check is validated if you get during control at least 10 times the right temperature If an adjustment is ne
142. dessin le 16 06 98 HEMATOLOGIE Ce plan est la propri t de la soci t ABX et ne peut tre reproduit ou communiqu sans autorisation Diag 6 DAD 109A Flat cable 560mm 14pts 8 56 SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 5 DAD112AS Flat cable 930mm 26pts Main board Carriage board a a lt en Li 9 lt cc lt LJ 1 LN JI MASTER BOARD HE10 F26 HE10 F26 CAH005A CAH005A NOTE REFER TO THE FLAT CABLE MANUFACTURING PROCEDURE RCF013A USE THE FLEXIBLE FLAT CABLE TYPE 3M 3319 26 THE MARKING CAN BE REALISED ON THE CONNECTORS Reduce the length 960 become 930 mm for P80 P60 common use 03 28 01 Add note for marking instruction 11 23 98 4203 B Creation for 10 14 98 JA hkl Date ECR Rev PENTRA MASTER BOARD CARRIAGE BOARD DIAGNOSTICS N DAD112A_ Sheet S This drawing is the property of ABX and may not be reproduced or distributed without authorization C Diag 7 DAD112AS Flat cable 930mm 26pts 9 56 PENTRA 80 TECHNICAL MANUAL RAAO22B 3 6 DAD119AS Flat cable 950mm 8pts P20 P4 E i Fa 215083 8 AMP 215083 8 CCA041A CCA041A J20 MOTOR BOARD C3 Cr lt e Z I 2 t or c lt ES Conductors dee NOTA REFER TO THE FLAT CABLE MANUFACTURING PROCEDURE RCF013A USE THE FLEXIBLE FLAT CABLE TYPE 3M 3539 10 OR 3319 10 Description Chk By Date ECO Re
143. detected Stop rack Transfer switch detec ted Mixer mechanism not reaching home Mixer motor failure Mixer Bad graber position Mixer Bad graber position Rack in rong side position No rack Unloading area full Tube too high in rack Bad rack transfer movement left Bad rack transfer movement right Front cover open Sampler Loading Motor Mecha nism initialization failed Sampler Loading Motor home switch always detected Sampler Loading Motor Sampler Loading Motor Sampler Transfer Motor Mecha nism initialization failed Sampler Transfer Motor home sensor always detected Sampler Transfer Motor End transfer rack switch not detec ted Sampler Transfer Motor End transfer rack switch always detected Mixer Motor Mechanism initiali zation failed Mixer Motor home sensor always detected Mixer Motor Grabers sensor position not detected Mixer Motor Grabers sensor position detected Rack in the wrong side in loa ding area No Rack in loading area Unloading area full Tube too high tube may not be correctly inserted in rack Movement control Movement control Front cover open ALARMS amp ERROR LIST Run an initialization Run an initialization Check motor in Service Menu Run an initialization Check motor in Service Menu Run an initialization Check motor in Service Menu Run an initialization Check motor in Service Menu Run an initialization Check motor in Service Men
144. djustment window enter the movement values Move Forward 1 Open Close Grabbers Move Back Home Move Forward 2 noticed on the new mixer and previously noted down Mixer adjustments are factory made and parameters are writted on a label on the mixer body PENTRA 80 Technical Adjustment Diag 6Technical adjustment screen e Press the Check Mixer button The mixer run the movements one by one e Check each movement and press Enter after each to launch the next one 4 6 MIXER REPLACEMENT RAS346B If the serial number is lower than P800154 check the following grabber positions procedure A e Check the Transfer mechanism home see RAS347B 3 1 Transfer home e Check the First transfer movement see RAS347B 3 4 First transfer e Control the mixer vertical position as follow Enter Menu Service Technician Menu Gains Sampler adjustment Press the Check mixer button the grabber move forward with the grabbers open Check the vertical position of the grabbers regard to the rack See Diag 7 Vertical position page 5 The lowest finger of the grabber must be centered between the fingers of the rack If it isn t the case loosen the 3 CHC mixer fixation screws then adjust this position Grabber AEN Side view Diag 7 Vertical position e Control the horizontal position To check the correct position of the grabber place 2 tubes into the rack position 7 and
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146. e 12 cl bleach to 1 part of bleach for 4 of deionize water 1 5 Instrument environment must be cleaned No sponge nor cloth must be used Only absorbant paper thrown after use in contamination bins have to be used For small or sensitive assemblies use accurate drier papers All assemblies suspected to have been in contact with biohazardous material must be disinfected with diluted bleach the stainless steel must be bleached below 30 Celsius Blood stains or salt marks must be cleaned with spray detergent first Reinstall all the assemblies and setup the instrument back to its initial configuration 8 8 p iitra 80 Decontamination and rinse Procedure RAS328B DIAGNOSTICS e Concerns Instrument decontamination before maintenance operations in the following cases e Instrument removed from biohazardous area e Maintenance intervention on contaminated assemblies e Required tools e Hexagonal keys e Clamps e Flat scrw driver e Torx keys e Required products e Fungicidal bacterial virus killing detergent spray non corrosive for metals non plastic altering e Bleach solution 12 C e Deionized water e Distilled water e Absorbant paper Intervention time 1h30min e Frequency On request Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 4 PENTRA 80 TECHNICAL MANUAL PROC
147. e 6 Diag 8Coaxial disconnection Disconnect both tube from the T connector few diluent drops may leak See Diag 9 T disconnection page 6 Diag 9 T disconnection Replace the coaxial cable 6 12 YEARLY MAINTENANCE RAS326B 7 Optical bench lamp 6V voltage check Run a Rinse cytometer cycle to get ride of air bubbles stuck to the inner optical surfaces From menu Service Menu Super User Hydraulic Clean cycle Rinse cytometer Check that the flowcell contains no or just a very few air bubbles From menu Service Menu technician Measurment LMNE adjustment Click on Run LMNE Adjustment button Mix the RBC PLT latex thorougly Closed tube holder when the message Please sample LATEX appears PENTRA 80 Technical Measurement 1 _ 190 00 Diag 10LMNE lamp 6V adjsutment Check LMNE lamp voltage is 6V 0 5V Check as well the other values are within the acceptable range Table 2 optical bench adjustment target values LMNE Lamp 6 00V 5 50 to 6 50 LMNE res 50 45 55 LMNE Abs 170 Set to maximum LMNE Transfer 200ms 190 t0 250 7 12 PENTRA 80 TECHNICAL MANUAL PROCEDURE 8 Diluent reservoir Joint amp Washer replacement 286 5 From menu Services Super User Menu Hydraulic Unprime Cycle run the All option to drain the chambers and the diluent reservoir Remove the righthand side panel and the mother board cover from the instrument See procedure RAS342 Front p
148. e II 3 ABX Eosinofix 4 ABX Cleaner 5 ABX Diluent 6 Waste container Diag 8 Reagent location Risk of erroneous results if diluent container is installed further than 80 cm 81 5 in bellow the instrument Diluent input tubing Cristal 3x6 2 meters 80 in maximum Waste output tubing Cristal 4x6 2 meters 80 in maximum 3 First start of the instrument Start the instrument ON OFF Switch is on the righthand side of the instrument Wait for computer s initialization When logon window is opened Log as administrator password XXXXX Beware to the type of the keyboard used Qwerty or Azerty When Pentra 80 is started it initializes its mechanical components wait until the end of the initialization 3 1 Racks The Pentra 80 racks are identified by means of barcode labels Prepare 2 or 3 racks to use during installation These labels have to be placed on racks as follows 8 10 PROCEDURE RAS324B Diag 9 Rack s labels front side 0102 0112 B 12 x 0113 P 03 13 0104 4A 0114 Diag 10 Rack s labels rear side 3 2 Check keyboard setup Check the type of the keyboard used Qwerty or Azerty From window Input Locales menu Settings System Local Settings press Edit button then Change Input Locales button 3 3 Check Date Time Check and if necessary adjust Date and Time From window Date Time menu Settings System Local Settings press Edit button then C
149. e Specific kit or consumables Power supply DBNOO6A Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE Switch off the instrument Disconnect Power supply cable Remove cover See RAS342 Lift the bar up by unscrewing the right screw See Diag 1 Bar screw page 2 Diag 1 Bar screw Disconnect on the Power supply the supply connector See Diag 2 Power supply connector page 2 Diag 2 Power supply connector Unscrew the 2 front screws of the Power supply See Diag 3 Power supply front screws page 3 2 4 POWER SUP PLY REPLACEMENT RAS339B bo S 7 E 1 4 SUCHE 1 11 13 1 1 11J1 1J 1 7 T T TU Diag 3 Power supply front screws Unscrew the 2 back screws of the Power supply See Diag 4 Power supply back screws page 3 Diag 4 Power supply back screws Remove the Power supply Reassemble following previous steps backward Switch on the instrument Check following voltages on mother board See Table 1 Power supply voltages page 3 and See Diag 5 Test points page 4 Table 1 Power supply voltages 40 5V Power supply 5V TP41 15V Power supply 15V TP42 24V Power supply 24V TP43 15V Power supply 15V 3 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE 111011111111 j i Yu e 03 3 D
150. e an effect on the MCHC and may cause inaccurate values RDW Red blood cell Distribution Width e The red blood cell distribution width is determined according to the RBC count e Nutritional deficiency or blood transfusion May cause high RDW results due to iron and or cobalamin and or folate deficiency Pit Platelets e Very small erythrocytes microcytes erythrocyte fragments schizocytes and WBC fragments may interfere with the proper counting of platelets and cause elevated Plt counts e Agglutinated erythrocytes May trap platelets causing an erroneously low platelet count The presence of agglutinated erythrocytes may be detected by observation of abnormal MCH and MCHC values and by careful examination of the stained blood film e Giant platelets in excessive numbers may cause a low inaccurate platelet count as these large 9 10 PENTRA 80 TECHNICAL MANUAL RAAO22B 10 10 platelets may exceed the upper threshold for the platelet parameter and are not counted e Chemotherapy Cytotoxic and immunosuppressive drugs may increase the fragility of these cells which may cause low Plt counts Reference manual methods may be necessary to obtain an accurate platelet count e Hemolysis Hemolysed specimens contain red cell stroma which may increase platelet counts A C D blood Blood anticoagulated with acid citrate dextrose may contain clumped platelet which could decrease the platelet count e Platelet agglutination C
151. e of paper See Diag 9 Lamp control 2 page 5 Diag 9Lamp control 2 A small screw 1 2mm allen key allows adjustment of the lamp position See Diag 10 Lamp adjustment screw page 5 Diag 10Lamp adjustment screw A This adjustment is factory made and must not be changed 5 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 6 6 p ntra 80 Front panel amp covers dismantling Procedure RAS342B DIAGNOSTICS e Concerns Front panel amp covers dismantling e Required tools e Hexagonal keys e Flat screw driver e Required products None e Intervention time 30min Frequency On request e Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE Switch off the instrument Disconnect power supply cable Pull Front left cover down 1 then remove it 2 See Diag 1 Front left cover page 2 Diag 1 Front left cover Pull Front right cover down 1 then remove it 2 See Diag 2 Front right cover page 2 Diag 2 Front right cover 2 8 FRONT PANEL amp COVERS DISMANTLING RAS342B Open the Right door place the key inside the screw slot and turn the captive screw counterclockwise See Diag 3 Right door page 3 Diag 3 Right door Remove Right side cover by unscrewing the 9 CHC M4X8
152. e of the WBC histogram Erroneous hemoglobin results will cause the results of the MCH and MCHC to be incorrect as well e Fetal bloods The mixing of fetal and maternal bloods may produce a high inaccurate Hgb value Hct Hematocrit e Red blood cells agglutination May produce an inaccurate Hct and MCV values Red blood cell agglutination may be detected by observing abnormal MCH and MCHC values as well as by examination of the stained blood film In such cases manual methods may be required to obtain an accurate Hct value MCV Mean Corpuscular Volume e Red blood cell agglutination May produce an inaccurate MCV value Red blood cell agglutination may be detected by observing abnormal MCH and MCHC values as well as by examination of the stained blood film In such cases manual methods may be required to obtain an accurate MCV value e Excessive numbers of large platelets and or the presence of an excessively high WBC count may interfere with the accurate determination of the MCV value In such cases careful examination of the stained blood film may reveal the error MCH Mean Corpuscular Hemoglobin e The MCH is determined according to Hgb value and the RBC count The limitations listed for the Hgb and RBC will have an effect on the MCH and may cause inaccurate values MCHC Mean Corpuscular Hemoglobin Concentration e The MCHC is determined according to the Hgb and Hct values The limitations listed for the Hgb and Hct will hav
153. e plastic protection under the reagents e Remove the lefthand side panel of the instrument 4 screws e Remove the reagent frame under the plastic protection 5 screws e Remove the sampler loader inox plate 4 screws e Remove the motor board plastic protection 2screws For more details about the dismantling of the instrument covers refer to procedure RAS342 Front panel amp Covers dismantling 2 4 MOTOR BOARD ADJUSTMENT RAS332B 2 Motor board general view MAIN BOARD POWER SUPPLY _ _ a P ps Iiv e SS MOTOR F REAGENT 0 Looneo 23 Reagent syr motor current an RIG SS 3 MOTOR E L INJECTOR IT_6 MOTOR HOME SYRINGES Injector syr motor current R23 Q GND SA SS 2 MOTOR mo F COUNTING T 98 i jm IT 3MOTORHOME Long i Le Counting moter current wo __ SS 2 MOTOR x I DRAINING 2 3 a ae 2 Sa emmm I short Draining 2 motor current SS 1MOTOR SAMPL HORIZ IT 1 MOTOR HOME CARRIAGE 8 z Carriage motor current a SS 1MOTOR a DRAINING 1 SYRINGE d d MOTOR _ Draining 1 motor current x SW T 1MOTOR HOME F ACK E TRANSFER are Transfer motor current gt o M1 RACK LOADING MOTOR Loading motor current Rack Transfer amp Loading rer Ss Lf SWITC
154. ectly measured for Hct Mcv Mch Mchc Rdw Mpv Pct and Pdw 2 Physical specifications 2 1 Power requirements Power supply from 100 Vac to 240 Vac cere 50 Hz to 60 Hz e Power consumption Maximum 230 VA e Printer Depends of printer face See printer s manual 2 2 Operating temperature and humidity e 16 34 C 61 93 F room temperature e Maximum relative humidity 8096 for temperature up to 31 C 88 F decreasing linearly to 50 relative humidity at 40 C 104 F 2 3 Dimension and weight Dimensions 82 x 57 x 54 cm V 34 1 x 23 3 X 22 in VY CISA err 55 Kg 122 Ibs SPECIFICATIONS 3 10 PENTRA 80 TECHNICAL MANUAL RAAO22B 2 4 Minimum specimen volume e CBC Mode CBC CBC 5DIFF Mode DIF 53ul 2 5 dilution ratios ANBC BASOS ES er eee 1 200 E 1 80 1 10000 eee ete eee 1 250 2 6 Hgb measurement e Hgb chamber LED 555 nm e Modified Drabkin method cyanmethemoglobin e Light source Electroluminescent diode e Wavelenght 550nm 10nm 2 7 Counting aperture diameters WBC BASO rennes 80um EV CRM 60um RBO PLT cnrs 50um 2 8 Reagent consumption ml Tab
155. ed by the passage of a cell through the micro aperture is directly proportional to the volume of the analyzed RBC The hematocrit is measured as a function of the numeric integration of the MCV 2 2 3 RDW calculation The study of the RBC distribution detects erythrocyte anomalies linked to anisocytosis A Red Cell Distribution Width RDW will enable you to follow the evolution of the width of the curve in relation to the cell number and average volume RDW K X SD MCV With e K system constant e SD Determined standard deviation according to statistical studies on cell distribution e MCV Mean Corpuscular Volume of erythrocytes 6 12 SECTION 4 ANALYSIS CYCLE TECHNOLOGY 2 2 4 MCV MCH MCHC calculation e MCV Mean Cell Volume is calculated directly from the RBC histogram e MCH Mean Cell Hemoglobin is calculated from the Hgb value and the RBC number e The mean hemoglobin weight in each RBC is given by the formula MCH pg Hgb RBC x 10 e MCHC Mean Corpuscular Hemoglobin Contained is calculated according to the Hgb and Hct values Mean Hgb concentration in the total volume of RBC is given by the formula MCHC g dL Hgb Hct x 100 2 2 5 MPV Measurement The MPV Mean Platelet Volume is directly derived from the analysis of the platelet distribution curve 2 2 6 Pct Calculation Thrombocrit is calculated according to the formula Pct Plt 103 ul x MPV um3 10 000 2 2 7 PDW calculation
156. ed in all the operations 1 10 PENTRA 80 TECHNICAL MANUAL INSTALLATION 2 10 1 Working conditions 1 1 Environment The Pentra 80 should be operated in an indoor location only Operation at an altitude over 3000 meters 9800 feet is not recommended Instrument is designed to be safe for transient voltages according to INSTALLATION CATEGORY Il and POLLUTION DEGREE 2 Please ask your ABX Diagnostics representative service center for any information about the operating location when it does not comply with the recommended specifications 1 2 Location The Pentra 80 should be placed on a clean and leveled table or work station Please note that the Pentra 80 printer and reagents weigh approximately 40 kilograms 88 lbs Avoid exposure to sunlight Proper ventilation requires that a space of at least 20 cm 8 inches must be left behind the instrument 1 3 Grounding Proper grounding is required Check that the wall ground earth plug is correctly connected to the laboratory grounding electricity installation If there is no ground then use a ground stake Current electricity Standards must be applied 1 4 Humidity and temperature conditions The Pentra 80 must function between 16 to 34 C 61 to 93 F Maximum relative humidity 80 for temperatures up to 31 C 88 F decreasing linearly to 50 relative humidity at 40 C 104 If it is kept at a temperature of less than 10 C 50 F the instrument should be allowed to
157. ee Diag 2 Chamber assembly dismantling page 2 Unscrew the 5 chamber assembly fixation screws See Diag 2 Chamber assembly dismantling page 2 Diag 2Chamber assembly dismantling Unscrew the CTN fixation screw from the heater See Diag 3 Heater disconnection page 3 After having recorded the tube location on the heater disconnect all the tubes See Diag 3 Heater disconnection page 3 2 4 HEATER ASSY REPLACEMENT RAS345B Diag 3Heater disconnection Disconnect CTN from main board plug in J31 See Diag 4 CTN disconnection page 3 BS 5 59 Sch e 15 E v Diag 4CTN disconnection Remove the heater 2 Heater replacement Install the new heater the same way in reverse order Use sleevings when necessary Tubes connected to the heater for example Make sure all the tubes are connected Enter new CTN value and adjust Heating coil temperature after replacement When new heater is installed start the instrument and check for no leak Enter the new CTN value Follow procedure RAS333 Temperature adjustment Check and adjust the MDSS adjustment Follow procedure RAS329 Chambers adjustment Complete a rinse cycle then prime all reagents Perform the Chekup after intervention procedure RAS340 in order to be sure the instrument works properly 3 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE 4 4 p ntra 80 Mixer replacement Procedure RAS346B DIAG
158. ement e 5DIFF syringe O ring replacement e Draining chambers O ring replacement e Optical bench Lamp amp LMNE coaxial replacement e Diluent reservoir e Counting chambers e Required tools e Hexagonal keys e Dynamometric screwdriver A302 A301 A300 e Cutting pliers e Flat screwdriver e Philips screw driver e Tork keys e Required products e Minoclair e RBC PIt Latex e Acetone e Intervention time 2h e Frequency See maintenance table e Specific kit or consumables Yearly maintenance kit XEA486CS Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 12 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 Yearly maintenance kit Table 1 Yearly maintenance kit XEA486CS DAJOO7A Optical bench lamp 1 FAA040A O ring 12 1x2 5DIFF syringe 1 FAA046A O ring 2 5x1 6 Coaxial cable 2 FAA064A O ring 14 2x1 52 Sampling syringe 2 FAA066A O ring 13 1x1 6 Draining chambers 4 FAAO067A O ring 2 4x1 9 5DIFF syringe 4 GBG275A Counting head EPO 0 5 joint 4 XEA663A LMNE flowcell coaxial kit 1 XEA286AS Waste chamber washer joint 1 XDA655DS Sampling needle internal teflon coating 1 JAJOO7A Polyeth Tape 1 5ML This procedure must be performed on a clean instrument If the instrument is suspected not to be perfectly clean perform a concentrated cleaning 2 Sampling needle replacement XDA655DS Switch off the instrument and disconnect the power
159. ent page 4 e Adjust the tube holder axis support in order to get a correct position of the tube holder and the needle e When done gently tight the 2 screws See Diag 5 Tube holder centering adjsutment page 4 e Open the tube holder e Finaly tight the 2 screws See Diag 5 Tube holder centering adjsutment page 4 and check adjustment again Repeat until you get a correct adjsutment Diag 5 Tube holder centering adjsutment The tube holder must have a little play about 1mm after the sampling position if not the tube holder may not open correctly check the presence of this play See Diag 6 Tube holder play adjustment page 5 4 10 TUBE HOLDER ADJUSTMENT RAS338B Diag 6 Tube holder play adjustment If necessary adjust tube holder s locker to get a correct play See Diag 7 Tube holder locker page 5 Diag 7 Tube holder locker Without this play because of the tube s cap the tube holder may not open correctly Tight the locker screws when adjustment is correct 5 10 PENTRA 80 TECHNICAL MANUAL PROCEDURE 4 Needle adjustment If the Teflon part of the needle comes into contact with the tube holder the needle will be A damaged When the adjustment of the tube holder is completed adjust the Tube Holder Position to get a correct needle sampling position From menu Services Technician menu Gains MDSS Adjustment Holder Adjustment press Control button The needle m
160. ent syringe Routes diluent to probe rinse block or heating coil Control basolyse II entry exit reagent syringe Routes diluent from flowcell and LMNE syringe to counting syringe Set counting syringe to atmosphere Opens vacuum count line for WBC and RBC chambers Drain for counting syringe Set draining syringe 2 to atmosphere through Rinse chamber Drain for draining syringe 2 Fill diluent tank or set diluent tank to atmosphere Probe cleaning Flushing out of probe rinsing block Direct diluent into interior or exterior of probe Probe cleaning Set draining syringe 1 to atmosphere through Rinse chamber Routes diluent to heating coil or sweep flow for counting head Routes diluent via heating coil to Hgb or RBC chamber Fill WBC Baso chamber with diluent Opens path from flowcell output to LMNE chamber for drain Entry of diluent Flushing Draining of the counting syringe Drain for rinse chamber Drain for first dilution chamber Drain for LMNE chamber Drain for RBC chamber Drain for WBC Baso chamber SECTION 2 HYDRAULIC amp PNEUMATIC PRINCIPLES 4 1 Cleaner circuit 1 ABX DJLUENT Lassssssssssssd v 5 D a Cum c a inani EH T SKIS ON MAY cs REINE TEET 54 059H XH H 1 x i menn E T SS sc e T i E ON a wamaqa s Se AAA 2 a
161. er and the lefthand side panel to access to the reagent syringe See RAS342 Front panel amp Covers dismantling procedure for further details Disconnect electrical connectors from the syringe then unscrew the 4 syringe s screws just few turns to free the silent blocks See Diag 10 Draining syringe 2 dismantling page 7 wA Diag 10Draining syringe 2 dismantling Follow the same procedure than for Draining syringe 1 to replace the piston see above 2 5 Counting syringe piston replacement GBG274A amp GBG260A Switch the instrument off and disconnect power supply cable Remove the cover and the lefthand side panel to access to the reagent syringe See RAS342 Front panel amp Covers dismantling procedure for further details Disconnect electrical connectors from the syringe then unscrew the 4 syringe s screws just few turns to free the silent blocks See Diag 11 Counting syringe electrical connections page 7 Diag 11Counting syringe electrical connections Follow the same procedure than for Draining syringe 1 to replace the piston see above 7 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE 3 Instrument cleaning Concerned assemblies e All the thermostated compartment e Outer surfaces of the instrument perpex covers reagent locations e Waste connector plug e Liquid valve push button e Assemblies close to the needle e Tube holder assembly e Overflow trays Cleaning Dilute th
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163. ews GBG169A Percutor ES OR C 3 7 PENTRA 80 TECHNICAL MANUAL RAAO22B 2 4 Fitting kit XEA311 kit includes most common pneumatic and hydraulic parts 2 5 Screws kit XEA293 kit includes most common screws used in Pentra 80 3 Maintenance procedures Maintenance and adjustments required for Pentra 80 are divided into procedures according to v the concerned assemblies This should make any update easier as all interventions can be done with the corresponding procedure on its own Each procedure has to be read entirely before starting intervention Procedures must be performed on a clean instrument If the instrument is suspected not to be perfectly clean perform a concentrated cleaning Disposable gloves should be worn 3 1 Required tools and products Table 5 Tools and products Tools Product Designation Barflex Clamps Cutting pliers Distilled water Dynamometric screwdriver A300 1 Dynamometric screwdriver A301 MAGO20A Dynamometric screwdriver A302 MAGO 19A Empty sample tubes Felt pen Flat screwdriver Grease for mechanical assemblies XEAS81A Hexagonal keys RBC PLT Latex LADOO2AS Liquid soap Micropipette tip Pair of scisors ocalpel Silicon grease LAMOOAA ooft tissue Syringe 5ml Thermometer Torx keyx Voltmeter Gauge set MAJOOAA 4 7 SECTION 7 MAINTENANCE 3 2 Procedures chart table Procedure Procedure Concerns P n Designation Unpacking Working c
164. ews to remove Internal PC from the cover Unscrew the 6 CHC M4x6 screws then remove the plate to access to the interface board See Touch screen interface board page 2 Diag 1 Touch screen interface board Disconnect the flat cable from the Touch screen interface board See Touch screen connection page 2 Gently pull the black part of the connector up to free the flat cable TI IPLE 1441444 ter kkkkkKK EERE RERE B ae 1 1 1 11 Diag 2Touch screen connection Depending of the instrument different cases are possible 1 The touch screen is sticked on the screen with double side tape It will be difficult to remove the touch screen without broken it Insert a thin blade between the touch screen and the screen then unstick it Remove all the glue from the contour of the screen Clean the screen 2 The touch screen is fixed on the screen with 4 pieces of tape Cut or remove the 4 pieces of tape then remove the touch screen Clean the screen 2 4 PROCEDURE RAS353B 2 New touch screen installation Put the new touch screen on the screen the flat cable should be on the right side On new instruments a square located at the bottom of the screen allow to keep the vertical location of the touch screen Adjust the touch screen position to have its window centered regard to the window of the screen Fix the touch screen using the tape included in the kit cut 4 equal pieces T
165. ge 1_2 LV30_2 S 254 150 LMNE chamber_5 1 22 65 WISIS 2 DES 152 60 ES Bo o 60 Wiss 1292 100 AR IE 12 1 52 Is M1_1 photocell M1_2 photocell 162 15 Isolator 1 1 Isolator 1 2 210 500 LV18_1 W18 3 205 210 Drain syringe 2_2 LV18_2 S 254 150 9 2 oman 1 52 50 LV35_2 V397 1 52 130 M3_1 photocell M3_2 photocell T52 15 Isolator 3_1 Isolator 3 2 205 460 Count syringe_3 l S 1 65 150 ASS Bye 2 05 140 Count syringe_4 Count syringe_5 2 05 120 LV16_1 Wio 2 S 2 54 150 3 T9 1 2 54 225 2 T14_1 2 04 20 Waste output Waste output C 4x6 maxi 2000 Waste container 4 18 SECTION 2 2 Instrument tubing 2 1 Tube list Table 2 Tube list HYDRAULIC amp PNEUMATIC PRINCIPLES Y Connector Y Connector 1410 6 Connector T220 6 Connector 0 040 TYGON tubing 0 051 TYGON tubing 0 060 TYGON tubing EABO21A EABO26A 5 EAEOO5A EAEOO6A EAE007A 25 INS 2 3 14072 1 02 1 02 1 02 1 02 1202 13072 1502 1 02 1 30 1 30 1 30 1 30 o2 5o 1 52 o2 S2 852 poe 1552 j 192 1592 52 1192 152 152 192 2 1652 1192 1552 1 52 o2 10 19 85 115 130 150 1725 205 240 15 20 185 320 15 20 30 35 50 60 65 70 15 80 90 100 120 130 160 170 180 190 200 215 230 260 N N MISI ESG M uw N D DY N HM PO a HR A O1 O N 5 18 PENTRA 80 TECHNICAL MANUAL
166. ghthand side PC fixation screws page 3 amp See Diag 4 Lefthand side PC fixation screws page 4 Connect the PC s accessories See Diag 1 PC disconnection page 2 Connect the LED connector See Diag 2 LED connector page 3 Connect the main supply and start the instrument Logon and check the instrument works properly 4 4 p ntra 80 PC Hard Disk Replacement Procedure RAS349B DIAGNOSTICS e Concerns PC Hard Disk replacement e Required tools Allen Keys e Required products None e Intervention time 1 hour e Frequency On request e Specific kit or consumables e For Pentra 80 XAA510CS Kit e For Pentra XL 80 XAA546AS Kit Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 6 PENTRA 80 TECHNICAL MANUAL PC HARD DISK REPLACEMENT PROCEDURE If its possible save and or print configuration before following this procedure See RAS 357 Read technical note included in the kit before replacing the Hard disk Remove internal PC See RAS348 Unscrew the 6 CHC M4x6 screws to remove Internal PC from the cover Unscrew the 4 CHC M3x6 and the 4 CHC 4 6 screws See Cover plate fan page 2 Do not remove Left Angle Plate A in order to keep the PC Cover adjustment A Diag 1 Cover plate fan Disconnect fan and remove cover plate Unscrew the 4 CHC M3x
167. h AZ 2 5 washers See CD ROM drive screws page 4 Diag 7 CD ROM drive screws 4 6 PROCEDURE RAS352B Unscrew the 2 CHC M2x6 screws See CD ROM board page 5 and remove board from CD ROM drive there is a nut between CD ROM drive and board Diag 8 CD ROM board Change CD ROM drive Reference CBT013A and reassemble in reverse order Floppy disk reader connection page 3 Make sure that FPC cable is correctly plugged with the blue band in correct position See Do not tighten too much different screws in order to not damage CD ROM drive Make sure that the flat cable connector is correctly plugged on Hard Disk because 4 pin are free See Hard Disk connector page 5 Diag 9 Hard Disk connector 5 6 PENTRA 80 TECHNICAL MANUAL PC CD ROM DRIVE REPLACEMENT PROCEDURE 6 6 p ntra 80 PC Touch screen Replacement Procedure RAS353B DIAGNOSTICS e Concerns PC Touch screen replacement e Required tools Allen Keys e Required products None e Intervention time 1 hour e Frequency On request e Specific kit or consumables PC touch screen XAA511AS Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 4 PENTRA 80 TECHNICAL MANUAL PC TOUCH SCREEN REPLACEMENT PROCEDURE 1 Touch screen dismantling Remove internal PC See RAS348 Unscrew the 6 CHC M4x6 scr
168. hange Date Time button Adjust Date amp Time then press Validate button to save changes 3 4 Check printer configuration Check the printer used by the system is correctly defined Enter menu Settings System Printer press Edit button then Printer Properties button Check the name of the printer used in the Name field of the Print window If necessary select the right printer then press Validate button 3 5 Prime reagents Prime reagent using barcode reader to enter lot number and to update expiry date automatically menu Status Run a whole priming of all reagents to be sure of a correct priming menu Service Super User Hydraulical Prime cycles All reagents 9 10 PENTRA 80 TECHNICAL MANUAL INSTALLATION 10 10 3 6 Mechanic check Make sure all the panels are fitted on the instrument Check in menu Service Super User Mechanical Check Sensors that all the sensors are green Check rack is well transfered to the reception tray menu Service Technician Gains Sampler adjust then press Check Rack Transfer Moving Check MDSS is correctly adjsuted menu Service Technician Gains M D S S Adjustment press Check Needle Position The probe muste be as shown Diag 11 MDSS Adjustment Close the thermal door righthand side of the instrument Check Hgb blank value menu Service Technician Measurement Gain Adjustment press Hgb Blank Adjustment If necessary adjsut between ground and TP1 with R248 potentiometer on main
169. haracteristics of the RED BLOOD CELL and PLATELET counts Initial blood volume 10 ul Method Impedance Vol ABX DILUENT 2500 ul Aperture diameter 50 um 5 12 PENTRA 80 TECHNICAL MANUAL RAAO22B Table 1 RBC PIt dilutions Technical characteristics of the RED BLOOD CELL and PLATELET counts Final dilution rate 1 10000 Count vacuum 200 mb Temperature of reaction 35 C Count period 2 X 6 seconds Two successive dilutions are carried out Primary Dilution for RBC and Plt Blood ul 10 ul Vol ABX DILUENT 1700 dilution 1 170 Secondary Dilution RBC and Plt from the primary dilution Dilution ul 22 5 ql Vol ABX DILUENT 2500 dilution 158 8 Final dilution 1 170 x 1 58 8 1 10000 2 2 2 Hgb Measurement The hemoglobin released by the lysis of the red blood cells combines with the potassium cyanide to form the chromogenous cyanmethemoglobin compound This compound is then measured through the optical part of the first dilution chamber using a spectrophotometric technique at a wavelength of 550 nm Table 2 Hgb measurement Technical characteristics for the HGB MEASUREMENT Blood volume 10 ul Method Photometry Vol ABX DILUENT 1700 yl Wavelength 550 mim Vol ABX LYSE 400 complement ABX DILUENT 400 ul Final dilution rate 1 250 Temperature of reaction 35 C Results Final Hgb result represents Absorbance value obtained x coefficient of calibration Hct Measurement The height of the impulse generat
170. he probe is about 1mm out from the percing needle See Diag 3 Needle home position page 3 Piercing needle Sampling needle Diag 3 Needle home position Press OK to continue See Diag 4 Check needle position button page 3 Check Needle Position Press Enter to Continue Diag 4 Check needle position button 2 2 Needle Home Adjustment Check needle home position See 2 1 Needle Home Check page 2 If probe is too low gap gt 1mm increase Needle home number of step If probe is too hight gap 1mm decrease Needle home number of step Run the check needle home again to control the gap See above 2 3 Needle position check To check the right position of the probe you must see inlet as in diagram A and not as in 14 diagram where circles are not concentric 3 10 PENTRA 80 TECHNICAL MANUAL PROCEDURE A Right position B Wrong position Remove cover See RAS342 Enter Menu Service Menu Technician Gains MDSS adjustment See 1 MDSS adjustment menu page 2 Press Check Needle Position button See Diag 5 Check needle position page 4 Check Needle Position The sampling needle moves over the LMNE chamber Disconnect the tube connected to the inlet and check in front of the inlet the right location of the probe if the probe is well adjusted you will see it as in the following diagramm See Diag 6 Needle perfect position page 4
171. he tape allow to keep the Touch screen in position before to re install in the PC Cover Use a flat screw driver to connect the flat cable on the interface touch screen board See Interface board connection page 3 Do not forget to block the connector Diag 3Interface board connection Put the plate back then screw it using the 6 CHC screws Install the PC in its cover using the 6 CHC screws take care not to move the touch screen Install the PC on the instrument 3 4 PENTRA 80 TECHNICAL MANUAL PC TOUCH SCREEN REPLACEMENT PROCEDURE 3 Adjustment Turn on the instrument Enter Menu Service N Technician menu N Others System tools 3 1 For a Pentra 80 Press the Launch Windows Explorer button Enter Start Run then enter Calwin exe in the window and press OK See Run page 4 Exploring C File Edit View Tools Help ec 3 el s Re xi aE All Folders Contents of C Desktop My Computer i386 File Folder 05 24 2002 11 18 3 Floppy al Program Files File Folder 09 30 2003 09 18 ep Recycler File Folder 11 18 2003 09 10 HS Hga 0 EJ winnt File Folder 01 06 2004 14 35 8 29 E _ JAutoexec bat OKB MS DOS BatchFile 03 20 2001 23 50 A ji Valse E amp boot ini 1KB Configuration Settings 03 20 2001 17 03 RSA Public ca dep Config sys OKB System file 03 20 2001 23 50 A ea 9 lo sys OKB System file 03 20 2001 23 50 RHSA H
172. hreshold test points are located at the top of the Main board See Threshold page 7 FRI FXZ FX3 O Ce al s P I Eee Q Q ao FE R11 r OH o Ey adel e E m LMNE CIS Ng m a TP4 R161 d E TP44 OA 6 miom TP23 Diag 8 Threshold Check and adjust if necessary the threshold voltages according to the following table Table 5 Thresholds adjustment Baso 112712 RISZ 390mV 5mV RBC ele R158 390mV 5mV Plt TP2 R159 390mV 5mV LMNE CIS TPS R160 700mV 5mV LMNE OD R161 350mV 5 7 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE 8 8 p ritrago Motor Board Adjustment Procedure RAS332B DIAGNOSTICS e Concerns Motor board adjustment e How to access to Motor board e Motor board general view e Motor board check and adjustment e Required tools e Allen keys e Voltmeter e Required products None e Intervention time 15 min e Frequency On request Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 How to access to the Motor board The motor board is located under the sampler loarder system on the lefthand side of the instrument e Lift up the reagent cover and remove the bottles e Remove th
173. iag 5 lest points 4 4 p ntra 80 Check up after intervention Procedure RAS340B DIAGNOSTICS Concerns Check up and control of the instrument accuracy e Repeatability e Calibration e Control e Required tools None e Required products e Fresh and normal blood samples Calibration blood samples Control blood e Intervention time 45min e Frequency On request Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 Repeatability This procedure must be performed on a clean instrument If the instrument is suspected to not be perfectly clean perform a Concentrated cleaning The measurement of repeatability is based on the set of 10 results obtained from the consecutive analyses of the same blood sample CBC or DIF type analyses can be invoked combination is not supported with a limit of 35 results per test Beyond the 35th result data generated from new analysis will be disregarded To remain undisturbed the CV calculation the potential results containing defaults generated directly from the analyses channels are rejected In that case a dialog box informs the user From Quality Assurance Menu menu enter Within run menu and run several samples in DIF mode Check that obtained coefficients of variation are within the follo
174. iag 6 Check and validate page 5 Vacuum must be at 200mb 4 6 VACUUM ADJUSTMENT RAS334B O Cs po v _ PENTRA 80 Drain Z syringe Motor Diag 6 Check and validate Press OK button Disconnect the Barflex and connect the tube back on the counting syringe 2 2 Counting syringe vacuum adjustment Follow the Counting syringe vacuum check procedure See above Change the Vacuum value motor step If vacuum is too low increase step value Repeat vacuum check and step value adjustment until you get the correct vacuum 200mb Disconnect the Barflex and connect the tube back on the counting syringe 5 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 6 6 pentra Bubbling Adjustment Procedure RAS335B DIAGNOSTICS e Concerns Bubbling adjustment e Required tools None e Required products None e Intervention time 15 min e Frequency On request e Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 2 PENTRA 80 TECHNICAL MANUAL PROCEDURE Bubbling is factory adjusted and normally does not required further adjustment If an adjustment is necessary follow this procedure Enter Menu Service Menu Technician Gains Bubbling See Diag 1 Bubbling page 2 PENTRA 80 Technical Adjustment
175. igit Several standards of barcode labels with different encoding are available According to the model of barcode used by the laboratory it is necessary to define the settings of the read characters Following the barcode standards the most used in laboratories e Code 2 of 5 interleaved e Code 39 e Code 128 e Codabar Among those typologies some can be used with or without Check Digit The Check Digit is an added encoding digit located on the right part of the code and corresponds to an algorithm returned value that checks the integrity of the barcode reading The Check Digit is optional and is defined by the administrator of the barcode label edition software 3 2 Barcode configuration Previously to barcode configuration it is necessary to know the barcode models used in the laboratory and to note the Typology and the Check Digit exploitation Exemple 1 Note the label models used in the laboratory as shown Laboratory XXX Typology Check digit Code 2 of 5 interleaved No Code 128 Yes Codabar Yes 2 Log in as lt Technician gt while starting the Pentra 80 application 3 Open the lt Technician Others gt screen See Diag 3 Barcode configuration screen page 4 and perform the setting adjustment as follows e Open the tab 1 e Select the lt Code type 2 5 Interleaved gt e Check the lt Check Digit None gt box e Confirm pressing lt Accept values gt PENTRA 80 Others Technician LMNE
176. ion position frame frame O B Diag 3 Holding plates 2 3 Electrical amp Computer connections Check that the power supply module voltage of the instrument corresponds to the nominal voltage supply of the laboratory and the country Connect the power supply cable See Electrical connections page 6 Connect the printer See Electrical connections page 6 Power requirements A Analyzer 250W Printer OKI 4200 350W Host connector Printer connector LAN connector USB connectors Pentra XL80 only Pentra XL80 only Diag 4 Electrical connections Connect computer s devices Mouse Keyboard Barcode reader See Computer s connections page 7 and See Computer connectors page 7 6 10 PROCEDURE RAS324B Mouse Keyboard Diag 5 Computer s connections 1 VGA 6 Printer 2 Mouse 7 COMS External Barcode reader 3 Keyboard 8 COM2 Labo link 4 LAN 9 COM1 To Mother Board 5 USB 10 Power Diag 6 Computer connectors Route the computer s wiring under the frame of the Pentra 80 See Computer s wiring page 7 Diag 7 Computer s wiring 7 10 PENTRA 80 TECHNICAL MANUAL INSTALLATION 2 4 Reagents installation Use Luer connectors straws and stoppers supplied with the installation kit to connect integrated reagents diluent and wastes Bottles and container locations See Reagent location page 8 1 ABX Lyse 2 ABX Basolys
177. ition Mismatch Between the First and Second Barcode Tube Read on Rack d Pos d No diluent in analyzer reservoir Printer alarm Printer module closed QC Failed Rack Moving Left Sensor Error Rack Moving Right Sensor Error Rack not identified Reagent level too low for daily workload Reagent level too low to run a analasys Reagent level too low to run a rack Incoherent Results System System Sensor state Result failed Sensor state Sensor state Sensor state Sensor state Sensor state Sensor state Sensor state Barcode Incorrect level of diluent into reservoir Printer problem Software QC Failed Sensor state Sensor state No read of Rack Barcode Label Out of Reagent Out of Reagent Out of Reagent Run an initialization Run an initialization Run an initialization Run an initialization Run an initialization Run an initialization Run an initialization Run an initialization Run an initialization Run an initialization Run an initialization Run an initialization Run an initialization Check diluent level and run a prime diluent cycle Check printer No message Ckeck Quality Control data in QC screen Run an initialization Run an initialization Check barcode label Check reagent Check reagent and restart auto matic cycle Check reagent and restart auto matic cycle SECTION 6 ALARMS amp ERROR LIST Table 4 Environment error types and help messages Result
178. j ap ayaludosd e sa uejd aj Jjoo1o1v3u Fee Pal V g pul ZV86EVax JUSUS SAT juauia ej U011P31IPON Je 91 9090 353ue J310 VULN3d 90 3 XVO voljeubisaq V86Evax 9D0 ajqissod ajijad snjd ej 2142 n 8402 e ej ap 8jjneup e Saude ajnJJaj e sajIuaJJxa Z ne jeixeo2 a qed aj 2JON 3Ue13 14 LM 0 gt 7 8 NOIIVH3d0 OWJaU auler Od 04721 JI0U ZZ DMV 15 NOIL V833dO 088721 VLSCVGX IeIxeo 2 NOI V33dO Diag 26 XBA398B RBC amp WBC electrode coaxial cable 28 56 ELECTRIC amp ELECTRONIC PRINCIPLES 3 25 XBA399B LMNE flowcell coaxial cable SECTION 3 sues ANDIUNWWOD JInpoadaJ asja ynad au ajanas e ajaridajd ej Ysa uejd aj sapupu uw 5 Lgj deuJoj 7 01103 d 6tvgx NS WO 3480 VOHETHIPON 4uausjaAaH vj 05 AE A ECC EE 3 eJauatj 33ue 13 0 WHLN3d JANI 300NY Xv02 uotjeubisag 02 x Z EG HION OHH3HI 3NIVO vLODY SO RE Ov VESLVEX TAIX YOI SL 29 56 Diag 27 XBA399B LMNE flowcell coaxial cable RAAO22B lt Z lt lt Z I i co lt Z LL mm uoiesuo ne sues anbiunarao no 1 poudas 3443 gt d au y NEY 2491205 t 3 ut d 3 02 1 9 1
179. ld model Rotation of the Syringe body on new model See Diag 7 Draining 1 syringe opening page 5 Molded Syringe New model Machined Syringe Old model Diag 7Draining 1 syringe opening 5 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE 6 8 Prepare the piston On the new molded syringe the piston 1 is shorter See Diag 8 Pistons page 6 For new molded syringe install only the piston GBG274A 1 For old machined syringe use the piston GBG274A 1 the cross piece GBG260A 2 and use the CHC M3x20 screws from the kit Old piston Diag 8Pistons Use new O ring when performing 6 month maintenance at the same time O ring FAA017A Washer GBG148A XDA621A Reassemble in reverse order See Diag 9 Draining syringe 1 view page 6 Chamber body of the new syringe is directly screwed on the motor block When the body is reinstalled on the motor block it has to be rotated until the mark on the body is located between the two marks of the motor block Molded Syringe New model Machined Syringe Old model 1_Motor assembly 2 O ring FAAO17A Anti extrusion ring GBG219A 4 Washer GBG148A 5_ Syringe body 6 CHC M4x16 screws x4 Diag 9Draining syringe 1 view PISTON KIT REPLACEMENT RAS327B 2 4 Draining syringe 2 piston replacement GBG274A amp GBG260A Switch the instrument off and disconnect power supply cable Remove the cov
180. le 3 Reagents consumption iue Diluent Eosinofix Basolysell Cleaner Lyse Cycles duration s ml ml ml ml ml CBO Bib OAS 27 4 0 45 CBC 045 24 4 20 1 0 0 45 Prime DILUENT o 00 44 Prime T EOSINOFIX J Al 1 6 23 1 Prime Uu BASOLYSE 2 125 1 7 29 7 1 0 Prime CLEA m NER 124 127 Prime LYSE D 8 4 Prime ALL GAS 50 7 24 0 24 0 25 0 8 4 SIARIUP 2 285 Do 2 3 0 EN 0 95 1 blank cycle SHUT DOWN 255 S22 120 120 1142 0 49 Rinse CYTOMETER LUE 5 2 I I AUTOCLEAN 1733 27 6 120 120 1 0 0 50 MINICLEAN DAS 10 3 1 0 20 me 0 33 R 4 00 29 6 1 0 1 0 1 0 0 50 TED cleaning BACKFLUSH O24 4 10 SECTION 1 SPECIFICATIONS STARTUP cycle estimated duration and consumptions are given for one blank cycle control It could be a maximum of three cycles 3 Summary of performance data 3 1 Repeatability e Based on 10 consecutive samples of the same fresh whole blood sample without alarm Table 4 Repeatibility 296 at 10 x 109 uL RBC 296 at 5 x 10 uL Hgb 196 at 15 g dL Hct 45 Plt lt 5 at 300 x 10 uL 3 2 Linearity Table 5 Linearity Difference PARAMETERS AUSE Which ever is greater WBC 10 uL O to 120 x 109 uL 0 3 or 7 RBC 10 uL O to 8 x 108 uL 0 07 or 2 RBC 10 uL 8 to 18 x 10 uL Reportable range PLT 109 uL Oto 1 900 x 109 uL 10 or 7 PLT Concentrated mode 10 uL 1500
181. lines See Diag 2 Tube holder dimantling page 3 Unscrew the 2 fixation screws See Diag 2 Tube holder dimantling page 3 2 10 TUBE HOLDER ADJUSTMENT RAS338B B Diag 2Tube holder dimantling Remove the tube holder assembly Install the new tube holder assembly at the same place Use the marks previously done Tight gently tube holder fixations screws Plug the electro magnet command connector and replace the tyraps Close the tube holder to sampling position and check the tube holder axis support touch the rail behind See Diag 3 Tube holder pre adjustment page 3 E 4 P a g Th 4 3 E Diag 3Tube holder pre adjustment If necessary adjust by the mean of the 2 screws See Diag 2 Tube holder dimantling page 3 the position of the tube holder assembly to get a correct adjustment 3 10 PENTRA 80 TECHNICAL MANUAL PROCEDURE 3 Tube holder adjsutments Switch the instrument on and log as technician From menu Services Technician menu Gains MDSS Adjustment Holder Adjustment press Control button Put the tube holder back then close the door and check that the needle is correctly centered inside one of the tube holder s sampling hole Position 1 for example Diag 4Tube holder centering check If not adjust the tube holder position as follows e Untight the 2 screws of the tube holder axis support See Diag 5 Tube holder centering adjsutm
182. ll coaxial X LX X X x x RASS26 Diluent tank joint X X X X X X Counting heads X X X X X X Sampling probe replacement X X X Reagent pistons replacement X X X Counting and draining X X X RAS327 syringe pistons replacement SECTION 7 MAINTENANCE 2 Maintenance kits 2 1 6 month maintenance kit Table 2 6 Month maintenance kit XEA710BS PIN FAA013A O ring 1 80x1 20 Nitrile Rinsing block FAA055A O ring 1 07x1 27 Fluocarbon Rinsing block FAAOS7 O ring 5x1 Nitrile Rinsing block FAAO065A O ring 6 30x2 40 Silicon Reagent syringe XDA621A O ring 30 80x3 60 washer counting draining syringe 1 amp 2 XDA622A O ring 15 54x2 62 washer Reagent syringe XEAO19A Silicon grease NN GQ 4 2 2 Yearly maintenance kit Table 3 Yearly maintenance kit XEA486CS DAJOO7A Optical bench lamp 1 FAA040A O ring 12 1x2 5DIFF syringe 1 FAA046A O ring 2 5x1 6 Coaxial cable 2 FAA064A O ring 14 2x1 52 Sampling syringe 2 FAAO66A O ring 13 1x1 6 Draining chambers 4 FAA06 A O ring 2 4x1 9 5DIFF syringe 4 GBG275A Counting head 0 5 joint 4 XEA663A LMNE flowcell coaxial kit 1 XEA286AS Waste chamber washer joint 1 XDA655DS Sampling needle internal teflon coating 1 JAJOO7A Polyeth Tape 1 5ML 2 3 Pistons maintenance kit Table 4 Piston kit XEA711CS PIN GBCO30A Reagent syringe Piston 4 GBCO31A Heagent syringe Piston GBG260A Piston D 31 Spacer GBG274A Piston D 31 KAAOO9A CHC M3x20 Scr
183. llowing screen appears click on OK button See Diag 5 Auto calibration screen page 3 PENTRA 80 IN Do you wantto run an automatic calibration Diag 5 Auto calibration screen 3 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE New LMNE Balance coefficient is applied to the instrument See Diag 6 New coef page 4 PENTRA 80 Others Technician LMNE Calibration Cycles Calibration d Blank Values Burn in cycle Sel Op TestDate GBBaso GBLmne V Techni 04 18 2002 14 36 20 8 4 8 7 d v Techni04 18 2002 14 37 06 4 7 4 2 M Techni04 18 2002 14 39 03 4 7 4 1 Iv Techni04 18 2002 14 39 49 10 0 8 8 Techni 04 18 2002 14 43 03 10 0 9 2 GBBaso GBLmne Current Coef 1 0 85 New Coef 1 0 85 Mean 0 0 0 0 QD Version V0 33 04 18 2002 14 44 01 Diag 6 New coef 7 LMNE balance coefficient can be modified in the software 14 Press Calibration coefficients button change LMNE value then press Accept Values button See Diag 7 Calibration coefficients page 4 PENTRA 80 Others Technician LMNE Calibration Cycles Calibration Coefficients Blank Values Burn in cycle Internal Parameters Calibration Coefficients r LMNE Balance WBC 130 47 LMNE Threshold 1 50 00 K Cali PLT Concentrate 250 00 RBC 172 37 mor TE LMNE Threshold 2 ooo PLT Concentrate HB Max HCT 182 63 _ 24 sese H
184. lou ung EV 18u0 S 0103 WEGEVEX Wa P9a A LL LE v EE SOILSONIOVIQ uasumg ieJ gjeJaugr a3ueJ9 o 09 V31N3d 318v 4 YNILVIULNIA uorjeubisag NiUO A C IG LZ07H7Z80N6 01 13 30 V8lonvd lun 008 VLOO A rc 20 4Z HV 19 La v020v2 55 04 1200 Z 0E OMY LIVINDD VLI0V22 3cg LSF gHd 9 ND o ND ed SEd YsLSVW SEF Diag 24 XBA393A Upper fan 26 56 SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 23 XBA396A Infrared 260mm 26 WHITE VERTI CARRIAGE DETECT GND GND ILED PHR 4 JST J1 CARRIAGE BOARD ux LLI LJ LL J vU lt r ex lt K U I lt t P Lil gt lt 260 mm gt CONTACT AWG 30 24 SPH 002T P0 5S CCA018A CAX001A CCA020A 890 55 HONEY WELL 0890 55 IA J 2 10 98 CT Cr ation pour PENTRA Date DeslVis Modification Designation CELLULE IR 2 PATTES CABLEE JST Code XBA396A L 260 mm HEMATOLOGIE Ce plan est la propri t de la soci t ABX il ne peut tre reproduit ou communigu sans autorisation Diag 25 XBA396A Infrared 260mm 27 56 RAAO22B PENTRA 80 TECHNICAL MANUAL 3 24 XBA398B RBC amp WEC electrode coaxial cable uoryesiiogne sues anbiunwwo no yinposdas aya nad au ji X8V 3413005 e
185. lumped platelets may cause a decreased platelet count and or a high WBC count The specimen should be recollected in sodium citrate anticoagulant to ensure the anticoagulated character depending on agglutination and reanalyzed only for the platelet count The final Plt result must be corrected for the sodium citrate dilution effect However these platelet clumps do trigger flags L1 LL and LL1 MPV Mean Platelet Volume e Giant platelets that exceed the upper threshold of the Platelet parameter may not be counted as platelets Consequently these larger platelets will not be included in the instrument s calculation of Mean Platelet Volume e Very small erythrocytes microcytes erythrocytic fragments Schizocytes and white blood cell fragments may interfere with the proper counting and sizing of Platelets e Agglutinated erythrocytes May trap Platelets causing an incorrect MPV result The presence of agglutinated erythrocytes may be detected by observation of abnormal MCH and MCHC values and by careful examination of the stained blood film e Chemotherapy May also affect the sizing of Plts Blood samples collected in EDTA will not maintain a stable Mean Platelet Volume Platelets collected in EDTA swell depending on the time post collection and storage temperature LYM Lymphocyte count absolute value LYM Lymphocyte percentage e The Lymphocyte count is derived from the WBC count The presence of erythroblasts certain parasites
186. mat aa i D FEFE ka v Gum wl Sas tit E EG e EE E Boo T noi Elle E t me a B D ie um maso P E 08 ETE mH mimg QD g rit am e 1 BB Spe Ee me ff Bog JE O agp J J AN 2 wee ma Diery f Zale f DE Pp Pp J oO ODR Da cm neo PZ P P ae ao LO ET af lt lt gt e Diag 16 XAA456A Main board PENTRA 80 TECHNICAL MANUAL 18 56 ELECTRIC amp ELECTRONIC PRINCIPLES SECTION 3 3 15 XAA458A LMNE amplifier board eu m 88 mm msg e z e ely e E S C d e e C Diag 17 XAA458A LMNE amplifier board 19 56 PENTRA 80 TECHNICAL MANUAL 20 56 RAAO22B 3 16 XAA459A Motor board 1 E U7 D sa R TP5 cs1l Rea Re7 W mal C57 REF ES EZ uu C Prise trecea Rea R87 H a m U11 m CEJ CES CEE CBS i aN O s 8 TP7 frc U13 z U12 E Q CB1 Rug U15 E Rud U14 6 E CB7 L SS T au Es O CA kaka gt O Diag 18 XAA459A Motor board SECTION 3 ELECT
187. mber O ring replacement page 9 Diag 14Draining chamber O ring replacement Repeat for all the chambers the same operation See Diag 15 Draining chambers page 9 TRI Mn NE TN x M Diag 15Draining chambers 9 12 PENTRA 80 TECHNICAL MANUAL PROCEDURE Counting heads RBC WBC coaxial cable O ring replacement 2xFAA046A Make sure the chambers are drained if not run a Services Super User Menu Hydraulic Unprime Cycle All command to drain the chambers Switch the instrument off Put a piece of absorbant paper or cloth under the chambers assembly Record the tube positions before dismantling the chambers assembly Disconnect chamber s tubes except for the wastes Unscrew the 3 screws from the chambers assembly See Diag 16 Draining chambers assembly dismantling page 10 Diag 16Draining chambers assembly dismantling Dismantle the electrode loosening the 2 fixation screws See Diag 17 Electrode dismantling page 10 Diag 17Electrode dismantling Use a previously cut micropipette tip to replace the electrode O ring See Diag 18 Electrode O ring replacement page 10 Diag 18Electrode O ring replacement 10 12 Reassemble the electrode then repeat the same operation for the other electrode YEARLY MAINTENANCE RAS326B 11 Counting head aperture joint replacement 2xGBG275A Carefully dismantle the counting head See Diag 19 Counting heads dismantling
188. mit must not be used e Not transmitted must be checked if the barcode model is with Check Digit Transmit Start Stop must be set on Disabled e Codabar Start Stop Char disabled when the Transmit Start Stop option is disabled 3 4 Troubleshooting 1 Labels are never read Check the if the label is readable Check there is white gap of at least 3 4 millimeters before the first bar and after the last one Check that the typology of the label used has been defined in a tab of the Technician Others screen Check that the label is with Check Digit and that it has been defined as Check Digit Not transmitted 2 Labels are read but an added digit appears on the right side Label includes Check Digit but the option has been defined on None 5 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 6 6 p ntra 80 External barcode configuration Procedure RAS344B DIAGNOSTICS e Concerns External barcode reader test and configuration e Required tools None e Required products None e Intervention time 15 min e Frequency On request e Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 Barcode reader configuration check Check that barcode reader is working properly by means of reading test
189. ml of diluent e RBC PIt counting and Hgb measurement e Chambers cleaning and Hgb blank e Draining and filling of DIL1 chamber with 2 7ml of diluent e Measurement of Hgb blank Flowcell rinsing about 1 6ml of diluent e Draining and filling of RBC chamber with 2 5ml of diluent 3 12 PENTRA 80 TECHNICAL MANUAL RAAO22B 2 Measuring principles 2 1 Multi distribution sytem MDSS 2 1 1 CBC mode In CBC mode 30ul of whole blood is aspirated then delivered with reagents into chambers as follows m Diluent one specimen for the first RBC PIt dilution m Air and the Hgb measurement B DL discarded another specimen for the BASO WBC count m 1011 WBC BASO m 101 RBC Plt Hgb m 3yL discarded Diag 1 Specimen distribution in CBC mode 2 1 2 Diff Mode In DIFF mode 53ul of whole blood is aspirated then delivered with reagents into m Diluent chambers as follows one specimen for the first RBC PIt dilution E and the Hgb measurement m Spl discarded another specimen for the BASO WBC count m 25yL Differential the last specimen for the LMNE matrix m 10pL WBC BASO m lOyL RBC Plt Heb m 3yL discarded Diag 2 Specimen distribution in DIFF mode 2 1 3 Specimen distribution Specimen distribution in the chambers is carried out in a tangential flow of reagent which allows perfect mixing of the dilution and avoids any viscosity problems this multi distribution in a reagent flow is an ABX
190. ng chamber e Positioning the needle point into a tangential flux of 1ml of ABX EOSINOFIX and synchronised distribution of 25ul of blood Dilution Blood Volume 25 ul Eosinofix Volume 1000 ul Diluent Volume 1000 ul Final Dilution Rate 1 80 2112 SECTION 4 ANALYSIS CYCLE TECHNOLOGY e Translation of sampling carriage over Rinse chamber e Rinsing of the needles e Internal rinsing 0 8ml inside sampling needle External rinsing 0 5ml outside sampling needle e Internal rinsing 0 5ml inside sampling needle External rinsing 0 3ml outside sampling needle WBC and LMNE counting 1ml of diluent into LMNE chamber e Dilution transfer trough LMNE flowcell and counting during 12 seconds e WBC BASO counting e Translation of sampling carriage over DIL1 chamber e Sample of the 42 5ul of the first dilution e Translation of sampling carriage over Rinse chamber e Rinsing of the exterior of sampling needle with 0 4ml of diluent e Translation of sampling carriage over RBC and PLT count chamber e Distribution of the 42 5ul of first dilution into a flux of 2 20ml of diluent e Distribution of 0 3ml of diluent from the interior of the needle Dilution Initial Blood Volume 10 ul Reagent Volume RBC chamber 2500 ul Final Dilution Rate 1 10000 Hgb e Distribution of 0 36ml of ABX LYSE into DIL1 chamber while bubbling e WBC BASO chamber rinsing e Draining and filling with 1ml of ABX CLEANER and 1 5
191. ng motor current z r 55 2 MOTOR i 5 d IT amp MOTOR HOME an 7 Drawing M2 motor current 4 FEL 55 1HMOTOR EE SAMPL HORIZ sa 5 monet CARRIAGE f A T i Carriage motor current m 55 1MOTOR E h FP DRAINING mi i C 9 a pf E lemme pres Draining M1 motar current t es o o m Sw T 1 MOTOR HOHE s TRANSFER 1 Hz HOTOR Transler motor current e H1 RACK LOADING MOTOR SWITCHES Rack Transfer 8 Loading Qe Buopeols es Md ee at go 5 3 IN x gt Po EEE i ERE 5 EF w a P A rM 48 Diag 2 Motor board general view 3 Connectors and other boards 4 56 ELECTRIC amp ELECTRONIC PRINCIPLES SECTION 3 3 1 CBCOO8A Barcode reader UOIJEZIJOYING jnouJI pajngiJJsip Jo paonpo da J aq jou pue xgv AjJedoud ayy si siu SOLLSONSVIG ijuguljeaJ SSIUYDNOY 9Gt 0 32U8 3 01 pJepuejs YIGVIY 300 4V8 08d uorjeubiseq NOILYNINAT 1 G47 SHL OLNI A11238l0 3001 LON 0 WV4d 43SV 3HL OLNI ONOT 001 3001 dl GADVWVO 38 NVI VNILSY JHL NOILN3LLY ATIn338V2 ONIGVSY 3H1 NV313 LHIO H04 A THV 10938 MOGNIM DNIOV3H IHL 23H2 SILON 83A0J DNIG 13IHS 6 AS Q13IHS 8 u G 3V08 08d Sf A ND vis s QxL A3uD y FHM saaw
192. not stored Software Run an autoclean RS232 alarm RS232 external problem Check host connection E Sample ID 96d already in pro No message gress SIL communication module Software No message closed en Sensor state Run an initialization Position Tube Level Detection Sensor in D Sensor state Run an initialization Wrong Position Two racks with same ID d in Barcode No message transfer rail Unable to launch print module Software No message Unable to launch SIL communi Software No message cation module id Senior IU Wee sig ESOS Sensor state Run an initialization sob Sensor state Run an initialization Position Waste container full Waste Container Full WERE tart automatic cycle XB failed XB failed Ckeck XB in XB screen 5 User error types and help messages Table 5 User error types and help messages Instrument stopped by user Run an AutoClean Instrument stopped by user at the end of analysis Run an Initialization Instrument stopped by user at the end of rack 6 Expiration date error types and help messages Table 6 Expiration date error types and help messages Change reagent and restart automatic cycle QC Lot Nb Barcode s expira Check QC epiration date and ted use another QC Lot Reagent s s expired 5 6 PENTRA 80 TECHNICAL MANUAL RAAO22B 7 Analyzer internal barcode error types and help messages Table 7 Analyzer internal barcode error types and help messages
193. nstrument and check there is no leak Reassemble the covers then perform the RAS340 Check up after intervention procedure 12 12 p traso Piston kit replacement Procedure RAS327B DIAGNOSTICS Concerns Pentra 80 maintenance procedure e Sampling probe Percutor replacement e Reagents syringe Pistons replacement e Vacuum waste pump Piston replacement e Required tools e Hexagonal Keys e Dynamometric screw drivers A302 A301 A300 e Cutting pliers e Flat screw driver e Philips screw drivers e Torx keys e Required products Minoclair e Intervention time 2h e Frequency See maintenance table e Specific kit or consumables Piston amp Needle kit XEA711CS Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 Piston amp Needle kit Table 1 Piston amp Needle kit XEA711CS GBCO30A Reagent syringe Piston 4 GBCO31A Heagent syringe Piston GBG260A Piston D 31 Spacer GBG274A Piston DES CHC M3x20 Screws GBG169A Percutor ES O NES CO K This procedure must be performed on a clean instrument If the instrument is suspected not to be perfectly clean perform a concentrated cleaning 2 Maintenance 2 1 Percutor GBG169A replacement Switch the instrument off and disconnect power supply cable Open the righthand side door Move the carriage to a
194. nt reservoir with level detection sues anbiunuuoa jInpoJdaJ 2142 jnad au Xgv 205 ej ap ajalidoud ej ue d 300101 VW3H OES _ PA V j4eujo4 SVG09VOx veld Uone SIPON ae L ENCRE LONIT NOCW 1812 0762 11H14 dQ WOS 92U9J49J94 550 0 Ax cuui 77 0 JEU ZZDMY Joxejduay uorjea gt n4 TJUNS 51079090 33U 19 0 VUlN3d VS09VOX 200 INVITI 30 303123130 Vo jeubisag LIVINOD 95581 1n3440 4 VION V6 0ZZ V0c0V22 402015 0762 11H14 dd 55 04 1200 5 0 DMY 12V LNOD V 00H80 V810V22 26X72 83 1102 40 0E AH 09 MION Zz Q LSF 7 Hd n MOE VAOSZ VI QSv08 YOLOW AV134 9f Zo OND 70 OEAM 72 DMY 32V 18 220 af eee 0 0EA3 92 DMV MVE ZUU 2770 LMS Diag 48 XDA605A Diluent reservoir with level detection 50 56 SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 47 XDA751C 12 valves assy LV1 6 amp LV7 12 A VANNE3V 2 N 3 VANNE 2V NF 4W N 4 VANNE 2V NF W N DIAGNOSTICS Drw size A3 This drawing is the property of ABX and may not be reproduced or distributed without authorization 12 VALVES ASSEMBLY Sheet 1 5 SE LD 12 72 70 53 ChkDrw Date ECR Rev 2 pa c o C4 Diag 49 XDA751C 12 valves assy LV1 6 amp LV7 12 51 56 RAAO22B PENTRA 80 TECHNICAL M
195. ometer located on optical bench board See Diag 7 Optical bench board page 5 gt das 1 j ME N Wa e E HN N g k m gt dd ri gt ref 4 M hdc M BETA Diag 7 Optical bench board 2 2 ET UC V SG Test points on optical bench board near R414 potentiometer can be used to check 6V with a voltmeter Adjust Resistive gain LMNE RES to 50 by turning R136 located on mother board See Diag 8 Mother board page 6 5 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE Diag 8 Mother board Adjust Optical gain LMNE ABS to the maximum and above 170 Adjustment is made by lateral or axial reception gun movement use toothed wheel and lateral screw See Diag 9 Opical bench adjustment page 6 D Ve Ww 4 Diag 9 Opical bench adjustment EZ Mother board Check that Transfer time is in limits of range Transfer time is not adjustable and must be between 150 and 250 6 8 LMNE FLOWCELL ADJUSTMENT RAS336B Transfer time bargraph is used to ensure the correct Right Left location of the light rectangle in the flowcell When the optimal value is reached the transfer time is around 200 because of the constant height betweens guns alignment and flowcell This height is garanted by the washer under the flowcell always put them back even when the flowcell is
196. on e Assemblies close to the needle e Overflow tray Put back all the assemblies and setup the instrument back to its initial configuration 3 Analysis circuit decontamination Prepair 1 bottle containing 1 2 litre of bleach diluted to 1 part of bleach for 9 parts of deionize water 1 10 Prepair 1 bottle containing 1 2 litre of distilled water Replace the reagent bottles Alphalyse Basolyse II and Eosinofix by the diluted bleach bottles Switch the instrument on Run an All reagents prime cycle from menu Service Menu SuperUser Hydraulical Prime 2 4 DECONTAMINATION AND RINSE RAS328B cycles Fill a sample tube with diluted bleach to 1 part of bleach for 4 parts of deionize water 1 5 Run 15 Burn in cycles from menu Service Menu Technician Others Burn in cycle Enter 15 cycles in Total Cycles To Do then press button Start rack See Burn in cycle screen page 3 Services Others Technician Burnin cycle LMNE tr Cycles Calibration Factors Total Cycles Do 5 Sampling Number 5 Cycles Done 0 Cycles Remaining 5 Total Rack To Load S amp Version 0 32 2002 04 11 08 05 39 Diag 2Burn in cycle screen Leave the instrument operating until it stops 4 Instrument Rinse Run this procedure before the transport of the instrument after a demonstration or before a long period without functioning 1 Run an Instrument general cleaning 2 Remove straws from Reagen
197. on is also the Sampling position if you need to readjust the Sampling position move the tube holder instead of changing the CARRIAGE HOME gt value rad XN a T Diag 10 Carriage home position 5 10 PENTRA 80 TECHNICAL MANUAL PROCEDURE Check the gap between the tooth wheel and the front of the carriage then press OK at the following screen See Diag 11 Check needle position button page 6 PENTRA 80 Check Needle Position Press Enter to Continue Diag 11 Check needle position button If the Carriage home position need to be adjust change the Carriage home value then press the Check Carriage Home button again Use MAJ004A gauge to check the gap between the tooth wheel and the front of the carriage modified increase or decrease the Dil 1 Carriage position with the same number of steps not to loose the other adjustments Increase Dil1 Carriage pos if you increase Carriage home Decrease Dil1 Carriage pos if you decrease Carriage home The Sampling position in the Tube holder will change too Re adjust the Tube holder position to centered the needle in the Tube Holder s sampling hole See RAS338 By changing the Carriage home value the carriage positions will change If this position is 6 10 PROBE ADJUSTMENT RAS330B 3 2 Carriage position check Enter Menu Service Menu Technician Gains MDSS adjustment Press Check Carriage Position
198. onditions Instrument installation First start RAS324 Installation of the instrument RAS325 Rinsing block Reagent syringe Draining syringe 1 amp 2 Counting syringe Filter Sampling syringe O ring SDIFF syringe O ring Draining chambers HAS326 Yearly maintenance O ring Optical bench lamp and coaxe Diluent reservoir Counting chambers Piston amp Needle kit repla Sampling probe amp guide replacement Draining syringe 1 amp 2 Coun HAS327 U cement ting syringe Reagent syringe RAS328 Decontamination amp Rinse Instrument decontamination amp Rinse RAS329 Chambers adjustment Chambers assembly position check and adjustment RAS330 Probe adjustment Check and adjustment of the MDSS Hgb blank adjustment RBC PIt gain adjustment WBC Baso gain RAS331 Main board adjustment adjustment Drain sensors adjustment Motor current adjustment Thresholds adjustment RAS332 Motor board adjustment Motor board currents check and adjustment Reagent heating coil and thermostatic compartment temperature RAS333 Temperature adjustment adjustment Draining syringe vacuum check Counting syringe vacuum check RAS334 Vacuum adjustment Counting syringe vacuum adjustment RAS335 Bubbling adjustment Bubbling adjustment RAS336 LMNE flowcell adjsutment LMNE flowcell position adjustment RAS337 lp tel LMNE balance calibration and forced calibration ment Tube holder assembly replacement Tube holder adju
199. ormated floppy disk in floppy disk reader then click on Save Setting On Floppy button See Diag 1 Save restore settings screen page 2 to save analyser setting and follow the same procedure than above 2 4 SAVE AND RESTORE SETTINGS RAS357B If floppy disk is not formated it will be automatically formated y 1 2 Analyser setting restore Click on Restore Setting From Hard Disk button to restore analyser setting from Hard disk See Diag 1 Save restore settings screen page 2 Click on Validate button on following screen See Diag 4 Restore screen page 3 PENTRA 80 Diag 4 Restore screen Click on Validate button on following screen See Diag 5 Analyser setup restored page 3 PENTRA 80 N Diag 5 Analyser setup restored After clicking on this button you will be automatically logout Z Insert the Floppy Disk where setting has been saved Click on Restore Setting From Floppy button to restore analyser setting from a floppy disk See Diag 1 Save restore settings screen page 2 and follow the same procedure than for restore settings from Hard Disk 2 Workstation setting Follow the same procedure than for Analyser settings but click on Workstation Buttons See Diag 6 Save restore settings screen page 3 PENTRA 80 Save And Restore Settings Diag 6 Save restore settings screen 3 4 PENTRA 80 TECHNICAL MANUAL PRO
200. ou qnl JO 15 eqni ajquedwo7 PROCEDURE Ueinoenueu eu Ag paljddns jou 4e ons euus e sjdeooe eqni eu ejgeisol deo NOUWAA 420010 1nO JUUG O 0998002230 L00L00 Xav age Ueinoenueu eu Aq jou Je ons euus e sjdeooe eqni eu ejgeisol deo OUI 42010 NO WUS O L60L06 10915165 SUONIPUOT U Jaquinu Sud SIDNS JOA wed 9pow 19Jnjoejnue A 0 des jo d y uonisoq jopjou SNL IAE pessy ym noullAA tra IUG 4188981 Axl 5 5 SH JEWOD A SUOI IPUO7 Jaquinu SUOIPAI9SQO des d S1 5311S OA 71111999 Ms 9polw J91njoejnue N uonisog Japjoy eqni ejqer eiqesi pe jou Jeqqny deo uM NVIN lug VIG3 6M MLSOSO LA 19oefloueA Ouuru9 Apnoeuoo uedo jou J p ou egni y JO do y pue Jepjou eui eoeds JO y pue ss uxolu deo y JO esneoeg zou Yaqqny deo 1nougAA VIQ3 M 999 J ule1nopA q suonipuo mom SuI S1 5 911S WNNOLA AlIDDV ueg 9pow Jounjoeynueyy SUOIJEAIASGC des jo addy c uonisog 1 qnl z e qer lt D Z lt gt lt 2 lI LL lt ce Z TE A 8 10 RAS338AA TUBE HOLDER ADJUSTMENT 9 10 eui Aq paljddns jou Jexons IELUS e S1d999e qn1 deo INOUJIAA ewo
201. oves down to the level of a tube position Gently press and turn the tube holder check that the needle nearly touch the surface of the tube holder without any contact Turn the tube holder to its initial position f the adjustment is correct click on OK the needle moves up If not click on OK the needle moves up modify the number of steps in the field Tube Holder position increase number of steps if the needle is above the tube holder decrease number of steps if the needle is too low inside the tube holder Click on Accept Value the rerun the Control function 5 Opening speed adjustment From menu Services Super User Mechanical Holder adjustment Press the Holder Open Time button Close the tube holder When the tube holder is opened a value is displayed in the Time window This value must be contained between 700 and 800 If not adjust the spring s tightness using the CHC screw See Diag 8 Spring adjustment page 6 to get the correct value then repeat the same operation Diag 8Spring adjustment 6 Adjustment check Once all adjustments have been carried out check all the screws hadve been well tighten Run few samples in stat mode to check that tube holder operates correctly 6 10 RAS338AA deo Og NVIAN jus lwo sued WOT deo yM VIQ3 EM
202. pentra 80 Technical Manual P n RAAO22B PENTRA 80 TECHNICAL MANUAL RAAO22BA 1 Revisions Table 1 Table of Revisions 22 RAH912AA lt V1 01 31 03 02 RAA022B RAH986 XL 80 integration All 30 04 04 This document applies to the latest higher software version When a subsequent software version changes the information in this document a new issue will be released 1 1 Notice of liability The information in this manual is distributed on an as is basis without warranty While every precaution has been taken in the preparation of the manual ABX DIAGNOSTICS shall have any liability to any person or entity with respect to liability loss or damage caused or alleged to be caused directly or indirectly by the instructions contained in this manual or by the computer software and hardware products described herein 1 2 Trademarks Other product names mentioned within this publication may be trademarks or registered trademarks of other companies 1 3 Copyright 2002 by ABX DIAGNOSTICS All rights reserved No part of this book may be reproduced or transmitted in any form or by any means electronic mechanical photocopying recording or otherwise without the prior written permission of ABX DIAGNOSTICS ABX DIAGNOSTICS Parc Eurom decine Rue du caduc e B P 7290 34184 MONTPELLIER Cedex 4 FRANCE Tel 33 0 4 67 14 15 16 Fax 33 0 4 67 14 15 17 2 4 INTRODUCTION 2 Working
203. placement Sensors check amp adjust ment Save and restore settings RAAO22B Replacement of the computer s CD ROM drive Heplacement of the computer s touch screen Heplacement of the instrument s main board Heplacement of the motor board Check and adjustment of the instrument s switches oave and restore analyser and workstation settings SECTION 7 MAINTENANCE 3 3 Training department procedure list Procedure Procedure Concerns P n Designation RAS324 RAS342 RAS325 RAS326 RAS327 RAS334 RAS335 RAS333 RAS329 RAS330 RAS331 RAS336 RAS337 RAS346 RAS347 RAS356 RAS343 RAS338 RAS332 RAS348 RAS349 RAS350 RAS351 RAS352 RAS353 HAS357 RAS339 Installation Front panel amp Covers dis mantling 6 month maintenance Yearly maintenance Piston amp Needle kit repla cement Vacuum adjustment Bubbling adjustment Temperature adjustment Chambers adjustment Probe adjustment Main board adjustment LMNE flowcell adjsutment LMNE balance adjust ment Mixer replacement Automatic sampler Sensors check amp adjust ment Internal barcode reader adjustment Tube holder adjustment Motor board adjustment Internal PC replacement PC hard disk replacement PC mother board replace ment PC floppy drive replace ment PC CD ROM drive repla cement PC touch screen replace ment Save and restore settings Power supply replace ment Unpa
204. r system on the lefthand side of the instrument e Lift up the reagent cover and remove the bottles e Remove the plastic protection under the reagents e Remove the lefthand side panel of the instrument 4 screws e Remove the reagent frame under the plastic protection 5 screws e Remove the sampler loader inox plate 4 screws Remove the motor board plastic protection 2screws For more details about the dismantling of the instrument covers refer to procedure RAS342 Front panel amp Covers dismantling 2 Motor board dismantling Carefully disconnect all the connectors from the motor board Unscrew the 6 screws maintaining the board and remove it See Diag 1 Motor board dismantling page 2 Diag 1 Motor board dismantling 3 Motor board replacement Install the new board and tight the 6 fixation screws Connect all the connectors on the board Adjust motor board See RAS332 Motor Board Adjustment Put back the covers 22 pentra 80 Sensors check and adjustment Procedure RAS356B DIAGNOSTICS e Concerns Sensors check and adjustment e Required tools Allen keys e Required products None e Intervention time 2 hours e Frequency On request e Specific kit or consumables Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 10 PENTRA 80 TECHNICAL MANUAL SENSORS CHECK AND ADJUSTMENT PROCE
205. rd See Diag 4 Optical bench electronic board dismantling page 3 Diag 4Optical bench electronic board dismantling Untight the 4 fixation screws silent blocks of the Optical bench on the frame See Diag 5 Optical bench fixation screws page 3 Optical Bench board should be removed on the picture Diag 5Optical bench fixation screws Locate the new Optical bench and tight the silent blocks about 4 turns Do not tight too much the silent blocks check optical bench cannot be lifted Connect all the tubings and wirings previously disconnected in the reverse order 3 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 2 Control of the Optical bench 2 1 Emission gun control of position Remove the Optical bench cover See Diag 6 Optical bench cover dismantling page 4 Diag 6Optical bench cover dismantling Check that gap between the emission gun and the flowcell is around 3mm See Diag 7 Optical bench control page 4 Be carefull not to damage the flowcell nor the emission gun with the allen key Diag 7Optical bench control This adjustment is factory made and must not be changed 2 2 Optical bench lamp alignement control Put a piece of white paper between reception gun and flowcell See Diag 8 Lamp control 1 page 5 4 6 OPTICAL BENCH DISMANTLING amp REPLACEMENT RAS341B Diag 8Lamp control 1 Check that the projection of lamp filament is vertical on the piec
206. rections for use See user manual e Emergency First Aid If the eyes or skin come into contact with the reagent rinse with water If the reagent is inhaled or ingested call local anti poison center or contact doctor e Storage conditions Room temperature between 18 C 65 F to 25 C 77 F ABX DIAGNOSTICS Part number 0206010 4 6 ABX BASOLYSE II e Function This reagent lyses RBCs for the leukocytes and differential count of the polynuclear basophils Composition Acidic solution containing a lytic agent e Description Colorless aqueous solution e Physico chemical properties pH 2 4 e Handling precautions Avoid contact with eyes skin and clothing Wear laboratory gloves when handling the product The product may be harmful if ingested or inhalated Keep the bottle closed when not in use 7 10 PENTRA 80 TECHNICAL MANUAL RAAO22B 8 10 e Mesuring Principles See user manual e Performances See user manual e Results See user manual e Directions for use See user manual Emergency First Aid If the eyes or skin come into contact with the reagent rinse with water If the reagent is inhaled breath fresh air immediately If a large quantity is ingested drink water immediately Do not induce vomiting Call local anti poison center or contact doctor e Storage conditions Room temperature between 18 C 65 F to 25 C 77 F e ABX DIAGNOSTICS Part number 0906003 4 7 Waste handling precautions If req
207. reen pen PROCEDURE RAS324B 2 2 Transport and installation To operate the instrument correctly install it in a place satisfaying the following conditions e Place where the instrument and reagents are not thermally affected by direct sunlight and intense radiant heat e Place where it is not exposed to water or vapor e Place where it is flat and free from vibration or shock e Place where an independent power receptacle can be used e Use a receptacle different from the one used by a device that easily generate noise such as a centrifuge etc e Provide a space of at least 10 cm at the back of the instrument for arranging the power cable and pipes Install instrument lifting handles in their location See Lifting handles installation page 5 make sure they are correctly secured lift the instrument using the four handles up on to the installation bench Diag 1 Lifting handles installation Open the thermic panel righthand side of the instrument Remove the plastic rails retaining the carriage See Plastic rails page 5 Diag 2 Plastic rails 5 10 PENTRA 80 TECHNICAL MANUAL INSTALLATION Remove the Left panel door then the Upper cover to access to the optical bench For transport the optical bench is fixed on the frame with holding plates They have to be removed before starting the instrument and fixed as shown on next diagram See Holding plates page 6 Transport imise posit
208. rews and perform a Probe adjustment procedure 3 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE 4 4 pentra Probe Adjustment Procedure RAS330B DIAGNOSTICS e Concerns Check and adjustment of the probe e Required tools Gauge set MAJOO4A e Required products None e Intervention time 15min e Frequency On request e Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 10 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 MDSS adjustment menu Check that chamber assy is parallel to the carriage motion before checking or making an adjustment of the Probe position Follow CHAMBERS ADJUSTMENT procedure of this manual RAS329AA Probe adjustments can be accessed by Menu Service Menu Technician Gains MDSS adjustment See Diag 1 MDSS adjustment menu page 2 PENTRA 80 Technical Adjustment SS aj 21 caj AI Diag 1 MDSS adjustment menu A Check probe position before any adjustment 2 Needle adjustment 2 1 Needle Home Check Remove cover See RAS342 Enter Menu Service Menu Technician Gains MDSS adjustment See Diag 1 MDSS adjustment menu page 2 Press Check Needle Home button See Diag 2 Check needle home page 3 2 10 PROBE ADJUSTMENT RAS330B Check Needle Home Diag 2 Check needle home Check that t
209. rw rm pel NOT pull gently on brown part of connector 1 97 T aG TU Vu 797 7 REIS es BD RE RR i SNI TITTATTITIT EFTIFYX IJ Pull FPC flat cable 2 7 id bod ew x RB 1 i lt s pte c8 Wm equ ri d d der dn L Ji a Pints n T b J y r a k Bir 1 1 i ke Au V LEA me 7 Ruk La Ma a lt BS ak 2 1 a RC q SIP Saree E ENT ae wm EN ET n Ere pud EL p lt k gt E IT EM kawsa T Y KI irii MI 2 2c LI NN Nr PERUPSBRABRENM r BEL PAE Blue band of FPC cable in this position Diag 7 FPC flat cable connection Remove SDRAM Board See SDRAM disconnection page 5 2 Pull RAM Board up Diag 8SDRAM disconnection Change Mother Board reference XAA509BS and reassemble in reverse order Make sure that the flat cable connector is correctly plugged on Hard Disk because 4 pin are free A See Hard disk connector page 5 and that blue band on FPC cable Floppy disk reader connector is in the correct position on Floppy disk See Floppy disk reader connection page 3 and on Mother Board See FPC flat cable connection page 5 Diag 9 Hard disk connector 5 6 PENTRA 80 TECHNICAL MANUAL PC MOTHER BOARD REPLACEM
210. s Disconnect flat cable from Hard Disk then from CD ROM Drive See Flat cable disconnection 2 6 PROCEDURE RAS350B page 3 om VM rmn da n lu El merus ru nsa idi Le pit z s Diag 3 Flat cable disconnection Before to disconnect FPC flat cable pull gently on brown part of connector 1 to free the flat cable See Floppy disk reader connection page 3 Disconnect FPC cable 2 from Floppy disk Reader See Floppy disk reader connection page 3 Remove Drives Support with Hard Disk Floppy Disk Reader and CD ROM Reader pull gently brown part of connector up 1 Pull up FPC flat cable 2 Blue band of FPC cable in this position Diag 4 Floppy disk reader connection Disconnect Hard Disk CD ROM Drive 44 points flat cable 1 Power cable 2 use a flat screwdriver and the 2 50 points flat cables 3 and 4 on the daugther board See Mother board 3 6 PENTRA 80 TECHNICAL MANUAL PC MOTHER BOARD REPLACEMENT PROCEDURE page 4 Matt NUN B Diag 5 Mother board Unscrew the 4 CHC M4x10 screws See Mother board page 4 Remove TFT Board located under Mother board See TFT board page 4 B 9 8 P s m w w aa 58 E E nq C AME nma EL po Diag 6 TFT board Remove Mother Board and disconnect FPC flat cable See FPC flat cal 4 6 PROCEDURE RAS350B
211. se order See Diag 9 Draining 1 syringe view page 6 Chamber body of the new syringes is directly screwed on the motor block When the body is reinstalled on the motor block it has to be rotated until the mark on the body is located between the two marks of the motor block Molded Syringe New model Machined Syringe Old model 1_Motor assembly 2 O ring FAAO1 7A 3_Anti extrusion ring GBG219A 4 Washer GBG1484 5 Syringe body 6 M4x16 screws x4 Diag 9 Draining 1 syringe view O ring FAAO17A Washer GBG148A XDA621A v 6 8 6 MONTH MAINTENANCE RAS325B 3 2 O ring replacement on draining syringe 2 Switch off the instrument and disconnect power supply cable Remove cover See RAS342 procedure Disconnect electrical connectors on the syringe then unscrew the 4 CHC M4x16 syringe screws just few turns to release the silent blocks See Diag 10 Draining 2 syringe dismantling page 7 Diag 10 Draining 2 syringe dismantling Follow the same procedure than for Draining 1 syringe to replace O ring See above 3 3 O ring replacement on counting syringe Switch off the instrument and disconnect power supply cable Remove cover See RAS342 procedure Disconnect electrical connectors on the syringe then unscrew the 4 CHC M4x16 syringe screws just few turns release the silent blocks See Diag 11 Counting syringe dismantling page 7 Diag 11 Counting
212. stments Ue sulcus rA ju imeni Needle adjustment Compatible tube list Power supply replace RAS339 Dismantling and replacement of the power supply Check up after interven Check up and control of instrument accuracy Reapetability Calibra RAS340 tion tion Control RAS341 aL Mle Optical bench dismantling replacement and control amp replacement RAS342 ad prete o CIS Dismantling of all instrument s panels and covers mantling Internal barcode reader RAS343 Adjustment of internal barcode adjustment RAS344 Adjustment of external barcode configuration RAS345 Heater assy replacement Replacement of the reagent heating system RAS346 Mixer replacement Replacement of the mixer Loading mechanism check and adjustment Mixer mechanism RAS347 TE check and adjustment Transfer mechanism check and adjustment RAS348 Internal PC replacement Replacement of the computer RAS349 PC hard disk replacement Replacement of computer s hard disk PC mother board replace RAS350 ment Replacement and setup of the computer s mother board PC floppy drive replace RAS351 ment Replacement of the computer s floppy disk drive 5 7 PENTRA 80 TECHNICAL MANUAL Procedure Procedure Concerns P n Designation 6 7 HAS352 RAS353 RAS354 HAS355 RAS356 RAS357 PC CD ROM drive repla cement PC touch screen replace ment Instrument main board replacement Motor board re
213. supply cable Open the righthand side door Move the carriage to access to the rinsing block Remove the two rinsing block screws then lift the locker to free the needle See Diag 1 Needle dismantling page 2 Diag 1 Needle dismantling Heplace the needle then reassemble in reverse order 2 12 YEARLY MAINTENANCE RAS326B 3 Sampling syringe O ring replacement 2xFAA064A Switch the instrument off and disconnect power supply cable Open the righthand side door Move the carriage to access to the sampling syringe Disconnect the tubes from the sampling syringe and unscrew the 2 fixation screws See Diag 2 Sampling syringe dismantling page 3 LS Diag 2Sampling syringe dismantling Open the syringe Unscrew the 3 FX M3x12 screws See Diag 2 Sampling syringe dismantling page 3 Replace both O ring joints Use one drop of silicon grease between 2 fingers to lubricate O rings Reassemble in reverse order Sampling syringe screws tightening torque 400 mN m 56 8 Ozf in 3 12 PENTRA 80 TECHNICAL MANUAL PROCEDURE 4 5DIFF syringe O ring replacement FAA040A amp 4xFAA067A Open the lefthand side panel to access to the 5DIFF syringe Gently disconnect the tubes from the 5DIFF syringe and unscrew the 2 fixation screws See Diag 3 5DIFF syringe dismantling page 4 Diag 35DIFF syringe dismantling Open the syringe Unscrew the 2xFX M3x12 amp the 4xCHC M3x12 screws
214. t N XBA4SBA Folio Z Format A4 Ech 1 Dim en millim tres Ce plan est la propri t de la societe ABX il ne peut tre reproduit ou communiqu sans autorisation C lt D fD lt Diag 33 XBA458A Switch JST 300mm 35 56 PENTRA 80 TECHNICAL MANUAL RAAO22B 36 56 3 32 XBA459A Switch JST 400mm CCA018A PHR 4 JST 1 Fil blanc AWG 24 3 Fil blanc AWG 24 Cosses FASTON 2 8 protege cosses Creation 08 04 10 00 5306 A Modification D signation TORON SWITCH lg 400 UST Tol rance Generale Etat de Surf Matiere Ab Traitement DIAGNOSTICS Revetement N XBA4S9A Folio 7 Format A4 Ce plan est la propri t de la soci t ABX il ne peut tre reproduit ou communiqu sans autorisation Diag 34 XBA459A Switch JST 400mm SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 33 XBA460A Switch Jst 500mm CA018A PHR 4 JST 1 Fil blanc AWG 24 3 Fil blanc AWG 24 Cosses FASTON 2 8 protege cosses Creation CT DB 04 10 00 5304 A Modification D signation TORON SWITCH lg 500 JST Tol rance Se Etat de Surf Traitement DIAGNOSTICS Revetement Rev tements O XBA460A Folio Z Format A4 Dim en millim tres Ce plan est la propri t de la soci t ABX il ne peut tre reproduit ou communiqu sans autorisation Diag 35 XBA460A Switch Jst 500mm 37 56 PENTRA 80 TEC
215. t e Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 6 PENTRA 80 TECHNICAL MANUAL PROCEDURE 1 Reagent Heating Coil Check amp Adjustment Start the instrument Enter menu Service Technician Measurement Gain The following screen is displayed See Thermic adjustment window page 2 PENTRA 80 Adjustment Reagents Heating Thermostated Compartmen Reference CTN Value 5540 Reference CTN Value Temperature Recorded 55 Run Cycle Number r l Check Therrnic Temperature Recorded Run Cycle Number V Version 1 3 0 lo 03 16 2004 08 38 34 Diag 1 Thermic adjustment window Reagent Heating e Reference CTN value Input the CTN constant in this field e Temperature recorded Allows to modify the temperature instruction e Run Cycle Number Number of cycles to check temperature adjustment This control must be made with the thermal door closed and under stabilised thermic conditions At least 5 minutes after startup 1 1 Reagent heating coil temperature check Open the thermal door on the righthand side of the instrument Plunge deeply the thermometer probe through the LMNE chamber s inlet 1 See Reagent heating coil thermometer s sensor installation page 2 Diag 2Reagent heating coil thermometer s sensor installation 2 6 TEMPERATURE
216. t ae Un gt lt C cc lt C eo O LLI I M mm 2 COLLIERS 2 4x92 DBH001A Creation for PENTRA 80 CTH ADC 1 13 00 5304 Description Chk By Date ECO Rev Designation JST EHR amp PHR 4 HARNESS L 200 PENTRA 80 LED BOARD Standard tolerance Materials Material Treatment DIAGNOSTICS Coating N XBAA87A Sheet S This drawing 15 the property of ABX and may not be reproduced or distributed without authorization Diag 46 XBA487A LED JST 200mm 48 56 SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 45 XBA488A Waste level detection WASTE LEVEL DETECT _ NN DETECT 24 WHITE GND 9 TC GND GND J47 MAIN BOARD PHR 4 JST CCA018A CONTACT AWG 30 24 SPH 002T P0 5S CCA020A gt a D Fk K J LL I C3 J LL gt LL J LLJ I un lt R114554 CBY010A 2 COLLIERS 2 4x92 DBH001A COSSE M5 AWG 22 18 DAUO11A DESIGNATION GAINE THERMO NOIR 23 2 x 10 Creation for PENTRA 80 CTH ADC 01 12 01 5304 Description Chk By Date ECO Rev Designation WASTE LEVEL DETECTION HARNESS PENTRA 80 Standard tolerance rs N XBA488A Sheet S This drawing is the property of ABX and may not be reproduced or distributed without authorization Diag 47 XBA488A Waste level detection 49 56 RAAO22B PENTRA 80 TECHNICAL MANUAL 3 46 XDA605A Dilue
217. t bottles and plunge them into an empty bottle 3 Remove straw from Diluent cubitainer and plunge it into the empty bottle 4 Enter Service N Super User Menu N Hydraulic N Unprime Cycle then click the All button See Unprime cycle screen page 3 Diag 3Unprime cycle screen 3 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE 5 Press the Validate button then repeat this cycle a second time 6 Dry the straws using absorbant paper 7 Plunge the straws in a bottle full of distilled water 8 Enter Service Super User Menu Hydraulic Prime Cycles then click the ALL REAGENTS button See Prime cycles screen page 4 Diag 4Prime cycles screen 9 Press the Validate button then repeat this cycle a second time 10 Run several manual cycles 11 Remove the straws from the distilled water bottle then plunge them into an empty bottle 12 Run several ALL Unprime cycles See Unprime cycle screen page 3 to drain the instrument 4 4 pentra 80 Chambers Adjustment Procedure RAS329B DIAGNOSTICS e Concerns Chambers check and alignment e Required tools Allen keys e Required products None e Intervention time 15 min e Frequency On request e Specific kit or consumables None Disposal gloves and white coat must be worn by the operator Local or national regulations must be applied in all the operations 1 4 PENTRA 80 TECHNICAL MANUAL PROCEDURE
218. t too much the rinsing block screws 2 Reagent syringes O ring replacement 1xFAAO65A amp 4xXDA622A Switch off the instrument and disconnect power supply cable Remove cover See RAS342 procedure Disconnect the following tubes See Diag 4 Reagent syringe page 4 RAS325B 3 8 PENTRA 80 TECHNICAL MANUAL PROCEDURE valve 7 inlet 3 valve 8 inlet 3 valve 9 inlet 3 valve 10 inlet 3 valve 12 inlet 3 Diag 4 Reagent syringe Unscrew the 2 fixation screws and remove carefully the reagent syringes See Diag 4 Reagent syringe page 4 Push several times the piston over the waste container to drain the syringes The thickness of the washer is matched to each O ring thickness For this reason keep the O ring and the washer together Lyse piston O ring small one had no washer On a piece of absorbant paper open the reagents syringe 9x CHC M3x12 screws 2x FX M3x12 screws Replace all O rings and washers by new one from the 6 month maintenance kit Use one drop silicon grease in between 2 fingers to lubricate the O rings See Diag 5 Reagent syringe open page 4 Washer Diag 5 Reagent syringe open TIGHTENNING TORQUES A 9x CHC M3x12 400mN m 56 8Ozf in 2x FX M3x12 400mN m 56 8Ozf in Reassemble in reverse order Use a dynamometric screw driver to tighten the syringe See Diag 6 Reagent syringe view page 5 4 8 6 MONTH MAINTENANCE RAS325B e 1_ Reagent
219. tion in order to have detection when covers are closed Keep a little play beetween switches detection and switches mechanical stop Thermic Panel reference CAE006A Unscrew the two CHC M2x10 screws and adjust switch position in order to have detection when Thermic panel is closed Keep a little play beetween switches detection and switches mechanical stop 2 4 Waste Waste Control No adjustment available 9 10 PENTRA 80 TECHNICAL MANUAL SENSORS CHECK AND ADJUSTMENT PROCEDURE 2 5 Others Tube holder cam reference CAE006A Open tube holder completely and adjust the wheel of the left switch in the V of the left cam then screw the switches and the left cam Closed the Tube Holder and adjust the right cam to have the wheel of the right switch in the V of the cam then screw gently the right cam Open the Tube Holder then close it again Make sure that the switch detection comes at the same time of the Tube Holder locking and screw the cam Left switch Right cam Right switch Left cam Diag 19 Tube holder cam Home Loader switch reference CAE019A Push the slide against the Mechanical stop ring and adjust switch to have detection in this position and keep a play of 1mm on the switch See Loader switch page 10 ee SLIDE me a ao s Dr b m Mechanical stop Diag 20 Loader switch 10 10 p ntra 80 Save and restore settings RAS357B
220. tions on the rack Adjust this value to center rack position under the two Tracers followers Put the rack on Loading Tray Press Check Rack Transfer Movement button See Diag 1 Sampler adjustment menu page 2 Rack moves under Tracers followers then it run 25 motions on the left and 25 on the right The following screen shows the detection A and non detection B zones of the Tracer switches See Diag 9 Tracers detection page 8 Increase or decrease First Transfer value to center non detection zone B on bargraph If non detection zone is on right decrease First Transfer value If non detection zone is on left increase First Transfer value Adjust it to have 9 2 motions on each side of the middle bar See Diag 9 Tracers detection page 8 Tracers followers position adjustments are factory made and non detection zone B width is depending on tracers up and down position If you have to move left tracer for example during mixer replacement keep right tracer for reference 7 0 PENTRA 80 TECHNICAL MANUAL PROCEDURE A B A Sampler Utilities Rack motion RACK n r Diag 9 Tracers detection 8 8 p ntra 80 Internal PC Replacement Procedure RAS348B DIAGNOSTICS e Concerns Dismantling of the internal PC e Required tools Allen keys e Required products None e Intervention time 10 min e Frequency On request e Specific kit
221. to 5000 x 109 uL i OO a g dl 1 3 to 24 g dl 0 3 or 2 HCT 2 to 67 2 or 3 3 3 Carryover e Carry over was tested by analyzing samples with hight concentration of WBCs RBCs Hgb and PLTs Each sample was run in triplicate followed by three background cycles The 96 carryover is calculated using the following formula Background1 Background3 Carryover 2 ee a ns Sample3 Background3 Table 6 Carryover Level 43 64 8 56 25 94 Carryover Actual 0 00 0 00 0 05 028 Carryover Claimed lt 0 5 lt 0 5 lt 0 5 lt 0 5 5 10 PENTRA 80 TECHNICAL MANUAL RAAO22B 4 Reagents specification In order for the instrument to operate correctly hight quality reagents must be used ABX DIAGNOSTICS provides all the necessary reagents All these reagents have been registered by the A F S S A P S Agence Francaise de S curit Sanitaire des Produits de Sant according to the procedure relative to laboratory reagents used for biological analyses These reagents are used for in vitro diagnostics All these reagents are manufactured by ABX DIAGNOSTICS Rue du caduc e Parc Eurom decine 34184 MONTPELLIER CEDEX FRANCE Tel 33 4 67 14 15 16 Fax 33 4 67 14 15 17 4 1 ABX DILUENT e Function This diluent is necessary for the process involved in counting and differentiating the blood cells This reagent is used also to rinse the hydraulic parts of the instrument
222. u Run an initialization Check motor in Service Menu Run an initialization Check switch in Service Menu Run an initialization Check switch in Service Menu Run an initialization Check motor in Service Menu Run an initialization Check motor in Service Menu Run an initialization Check motor in Service Menu Run an initialization Check motor in Service Menu Set rack in the right side position and restart automatic cycle No message Unload racks and restart auto matic cycle Run an initialization Open left front cover and remove rack from loading area Close left front cover and restart automatic cycle Run an initialization Run an initialization Close front covers Run an initialization 3 6 PENTRA 80 TECHNICAL MANUAL RAAO22B 3 STAT mode error type and help messages Table 3 STAT mode error types and help messages 4 6 Tube holder mechanism failure Door not open 4 Environment error type and help messages Table 4 Environment error types and help messages No message d Incoherent s Result s for S Communication With Analyzer Cut Off Communication With Analyzer Failed End Sampler Transfer Sensor Error Error on raw results sending Holder Sensor 1 in Wrong Posi tion Holder Sensor 2 in Wrong Posi tion Holder Sensor 3 in Wrong Posi tion Holder Sensor 4 in Wrong Posi tion Loader Left Sensor Error Loader Right Sensor Error Lower Piercing Sensor in Wrong Pos
223. uired waste can be neutralized before being discarded Follow your laboratory s protocol when neutralizing and disposing of waste 5 Limitations 5 1 Maintenance In Chapter 8 Maintenance specific maintenance procedures are listed The maintenance procedures identified are mandatory for proper use and operation of the ABX PENTRA 80 Failure to execute any of these recommended procedures may result in poor reliability of the 4 system 5 2 Blood specimens Verification of any abnormal test result including flagged results or results outside of the normal range should be performed using reference methods or other standard laboratory procedures for conclusive verification of the results The sections below list Known limitations of automated blood cell counters which use the principles of impedance and light absorbance as principles of measurement 5 3 Known interfering substances WBC e White Blood Cells Leukocytes WBC results that exceed the linearity limits of the system will require dilution of the blood sample Leukemia sample followed by a leukopenia Re assaying the diluted sample will help to obtain the correct assay value e Unlysed Red Cells In some rare instances the erythrocytes in the blood sample may not be completely lysed These non lysed red blood cells may be detected on the WBC histogram with an L1 alarm or as an elevated baseline on the side leading edge of the lymphocytes population Non lysed erythroc
224. ull the switch down If non detection zone B is too large lift the switch up 8 10 PROCEDURE RAS356B 2 3 Unloading area Rack End Transfer reference CAEO1 14 Put a 0 5 mm gauge against Ejection Tray mechanical stop then push a rack against this gauge Loseen the two CHC M2x10 screws and adjust Rack End Transfer switch to have detection in this position then screw the switch See Rack end transfer adjustment page 9 7 Switch in detection position Put a 0 5 mm gauge here Ejection Tray 0 5 mm Diag 18 Rack end transfer adjustment Unloading Area Full reference XDA742AS This adjustment is the same that Stop Rack Loading Left and Right Sensors The switch is 14 prestressed the bracket See Bracket switch adjustment page 7 Reference of switch alone CAE019A Adjust the switch bracket assy in order to be sure that the switch axle hang not out of unloading tray See Switch wheel position page 6 Press a rack against the left side of Ejection Tray thermic panel side and push it from the front to the rear Adjust the switch bracket assy in order to get detection when the rack comes in front of the switch The Axle of the switch must not hang out of the Ejection Tray Plate in order to not damage switch if rack is for example manually moved Covers reference CAEO20A Front cover left and right reference CAE0204 Unscrew the two CHC M3x12 screws and adjust switch posi
225. up of the computer s mother board Heplacement of the computer s floppy disk drive Replacement of the computer s CD ROM drive Heplacement of the computer s touch screen oave and restore analyser and workstation settings Dismantling and replacement of the power supply 7 7 PENTRA 80 TECHNICAL MANUAL 8 7 RAAO22B Procedure Procedure Concerns P n Designation HAS341 RAS344 HAS345 RAS354 HAS355 RAS340 RAS328 Optical bench dismantling amp replacement External barcode reader configuration Heater assy replacement Instrument main board replacement Motor board replacement Check up after interven tion Decontamination amp Rinse Optical bench dismantling replacement and control Adjustment of external barcode Replacement of the reagent heating system Replacement of the instrument s main board Replacement of the motor board Check up and control of instrument accuracy Reapetability Calibra tion Control Instrument decontamination amp Rinse p ntra 80 Installation Procedure RAS324B DIAGNOSTICS e Concerns Working conditions e Instrument installation e First start of the instrument e Required tools None e Required products None e Intervention time 1 hour e Frequency On request e Specific kit or consumables Installation kit XEA785B Disposal gloves and white coat must be worn by the operator Local or national regulations must be appli
226. v K7 TRANSLATION EA1 PENTRA 80 Standard tolerance Roughness Material Treatment Coating Coating S S O N DADTI9A Sheet S This drawing is the property of ABX and may not be reproduced or distributed without authorization DIAGNOSTICS Diag 8 DAD119AS Flat cable 950mm 8pts 10 56 SECTION 3 ELECTRIC amp ELECTRONIC PRINCIPLES 3 7 DAD120A Flat cable 800mm 14pts HE10 F 14 HE10 F 14 CAH002A CAH002A J34 MASTER BOARD J23 PREAMPLI LMNE BOARD emm sum _ REFER T THE FLAT CABLE MANUFACTURING PROCEDURE RCFO43A sss sm sl Description Chk By Date ECO Rev PREAMPLI LMNE PENTRA 80 Ab Material Material Treatment DIAGNOSTICS Coating Coating S O N DAD120A Sheet S This drawing is the property of ABX and may be reproduced or distributed without authorization Diag 9 DAD120A Flat cable 800mm 14pts 11 56 PENTRA 80 TECHNICAL MANUAL RAAO22B 12 56 3 8 DAD121A Flat cable 700mm 44pts For example 91820 044 FCI BERG 3625 44 3M J1 MOTOR BOARD J36 MASTER BOARD 89947 344 FCI BERG 89947 344 FCI BERG CAH015A CAH015A OTHER CONNECTOR SOURCES IDS044W200 01 E tec 152244 0x10GB or 87044 1600 in molded socket 3M NOTE REFER TO THE FLAT CABLE MANUFACTURING PROCEDURE RCF013A Creation following prototype P80 GC ADC 05 02 02 po Description ChNkBy Date ECR Rev PENTRA 80 This drawing
227. wing limits Table 1 Within Run Coefficients of variation WBC lt 2 at 10x10 ul RBC lt 2 at 5x10 ul Hgb lt 1 at 15g dl Hct S at 4596 PIt 596 at 300x10 ul 2 Calibration Calibration function is used to determine the values of the calibration coefficients to calibrate the instrument with known result samples From Quality Assurance Menu press the Calibration button Select the calibrator lot number you are going to use for calibration in the left area of the window If the calibrator is not listed create it From the Calibration Target menu check target values are correct if not press Edit button beep your current calibrator barcode or type in the target values supplied with calibrator leaflet in the reserved fields then confirm by pressing Ok button Exit the Target Values by pressing Return button Prepare the calibrator according to the specific instructions detailed in the calibrator package insert temperature mixing Press the Stat mode button open the calibrator vial and install it the tube holder Close the door When the tube holder opens remove and recap the vial Hisk of erroneous results if the calibrator is not continously mixed between each analysis In order to obtain a correct calibration it is recommended to run at least 5 calibrator samplings When the analysis cycle ends the result is displayed on the result grid Run the calibrator 4 more times 2 4 CHECK UP AFTER INTERVE
228. x 0ZLd 09d 09d HAHSVM ONIHO Jovi 1N3n1iq 08 09d MNV L 110 019 LSWM YSEWVHO 08 09d XNV L LNANTIQ YASWVHO svozovax NOILVNO9IS3Q dIANNN LYVd XAQANI UolyeSiJoyne sues anbiunwwo no pinposdas au X8V 349190S ej ap ayatudoud uejd une V 8L 49Vd 9vig kO PO FZ 31vQ LET 08 09d HONd8 OlLdO 420 18 LN3 IIS dINV T 1WOILdO NOILVNOISIG 08 09d HON38 9ILdO 90184 IN3lIS VOLOTVJ E 08 09d HON38 HOS dNV1 1V9ILdO V400f VG m NOLLVNOIS3Q YAENNN LSVd Xa3cdNwl m PA j nda eee b Ju LET AAA AR rica i TE I phlei EI FE ir TELE Si EN PI de ees Kuya ef gee CES 1 Ir ina FET Hira LEE NTI TEE TER ee TT SEIS EL Eras TEL 1d EOS kL E 8s i i Z O lt Z LLI X LLI co gt Z X lt A LLI 2 AUTO THREADED SCREW 1 PCB LED BOARD FOR COVER DESIGNATION PCB LED BOARD FOR COVER DATE 16 01 02 DIAG PAGE 19 Ind NOTE TECHNIQUE PENTRA 80 Ce plan est la propri t de la soci t ABX il ne peut tre reproduit ou communiqu sans autorisation MODIFICATIONS sues anbiunuuo no yinposdas ynad au Xgy 242008 ej ap ayaisdoud ej ysa uejd a 08 Ve L N3d 670 IN
229. y CEP REPRE 8 7 Th db TO t pa TRES 12 18 HYDRAULIC amp PNEUMATIC PRINCIPLES 4 5 Diluent circuit SECTION 2 usva df cie 3ISVM Toi Fr a Pa La zl 298 d n Je ta SOINS EM Em 2 T a HID In 3547068 1 MLSNIEDI XY A cum 1 13 18 RAAO22B PENTRA 80 TECHNICAL MANUAL 4 6 Diluent prime circuit LING lia Mev 31SYA P 4 315VM 4 awan oT T Tu i es i PR 1 NE TR T ri 4 E i m nal i c E Il 18 d ERA RARRRRRPRPRPRP x ta HUY oS XLANISDI KEY 35A TETE May oe e XP pud BOO a SP a ee OGEZRTWAZGERREGARRSERRZRRERERRERRRRERRARRREREEZZEZEREEXZEZEZZXZZXZXXXH stt QTR SUR Ge die Ge CIS ite TE Ea 2 e R mmml Bm 14 18 SECTION 2 HYDRAULIC amp PNEUMATIC PRINCIPLES 4 7 Counting circuit pi ENT WASTE gt Lad fi E gt amp FRERES aaa H p a a Ti XisNISn3 MAY BE Ba Lv P ko 6 he eC 3 LN 2 a th 15 18 PENTRA 80 TECHNICAL MANUAL 16 18 4 8 LMNE transfer
230. y displayed every 700ms during a total of 27 seconds A Never dismantle Emission gun or even unlock it The adjustment is factory made and cannot be performed in the field Remove the cover See RAS342 Unscrew the cover of the optical bench See Diag 1 Optical bench cover page 2 Diag 1 Optical bench cover Run a Rinse cytometer cycle to get ride of air bubbles stuck to the inner optical surfaces Enter Menu Menu Superuser Hydraulic Clean cycle Rinse cytometer See Diag 2 Clean cycle page 3 2 8 LMNE FLOWCELL ADJUSTMENT RAS336B PENTRA 80 Hydraulic System Drain Chambers Prime Cycles Unprime Cycle Concentrated Cleaning BackFlush Rinse Cytometer 44 Version 0 33 04 25 2002 15 05 59 Diag 2 Clean cycle Check that the flowcell contains no or just a very few air bubbles Enter Menu service menu technician measurment LMNE adjustment See Diag 3 LMNE adjustment page 3 PENTRA 80 Technical Measurement Gain Adjustment x LMNE Adjustment Aperture Current Pulse Adjustment Run LMNE Adjustment Lamp Voltage Resistive Gain LMNE Abs Gain Average Time of Transfer 49 Version V1 3 0 03 16 2004 10 16 28 Diag 3 LMNE adjustment Mix the RBC PLT latex thorougly Click on Run LMNE Adjustment button Closed tube holder when the following screen appears See Diag 4 Sample the latex page 4 3 8 PENTRA 80 TECHNICAL MANUAL PRO
231. ytes will cause a falsely elevated WBC count e Multiple myeloma The precipitation of proteins in multiple myeloma patients may give high WBC counts Leukemia A very low WBC count may result from this disease because of possible increased fragility of the leukocytes leading to destruction of some of these cells during counting These white cell fragments will also interfere with the white cell differential parameters e Chemotherapy Cytotoxic and immunosuppressive drugs may increase the fragility of the leukocytes which may cause low WBC counts e Cryoglobulins Increased levels of cryoglobulin that may be associated with myeloma carcinoma leukemia macroglobulinemia lymphoproliferative disorders metastic tumors autoimmune disorders infections aneurism pregnancy thromboembolic phenomena diabetes etc which can increase the WBC RBC or Plt counts and the Hgb concentration The specimen must be warmed up to 37 C 99 F in a bain marie for 30 minutes and analyzed again immediately after analyzer or manual method e Macrothrombocytes in excessive numbers may affect and increase Leukocyte numeration SECTION 1 SPECIFICATIONS RBC e Red Blood Cells Erythrocytes The red blood cell dilution contains all the formed elements in the blood erythrocytes leukocytes and platelets During erythrocytes counting red blood cells platelets are not counted as their size falls below the minimum threshold e Agglutinated erythrocytes

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