BANG - INTRODUCTION
Contents
1. Setup in MPA Mode 2 3 2 Operating the BLAM in MPA Mode with External Refill If your experiment requires running the BLAM at high output for long periods of time it is recommended that the device be operated with the external refill option This allows the user to refill the BLAM with the test liquid without interrupting atomization Follow these steps to run the BLAM in MPA mode with external refill option 1 Remove the liquid feed port plug on the lid 10 11 12 Install the provided liquid feed barb and tighten it up If you will use a syringe pump for the refill fill the syringe with about 100 ml of test article and place it in the pump If you are using a low flow peristaltic pump instead fill a glass beaker with about 100 ml of liquid and dip the inlet end of the pump s tube in it Using a piece of thin silicon tube connect the outlet of a syringe or low flow peristaltic pump to the BLAM s liquid feed port refer to Figure 3 Next follow steps 1 through 7 in Section 2 3 1 to operate the atomizer When the liquid in the jar is almost exhausted turn on the external refill pump When the jar 1s refilled with the predetermined amount of liquid turn the pump off ATTENTION Make sure that all the connections between the external refill pump and BLAM air tight Repeat the refill procedure as often as needed during the atomization process Shut down compressed air supply to the atomizer when you a2. occlusion from solids that may dry inside the nozzle and or the nozzle is free from any residuals that may cause cross contamination To do this after cleaning the parts externally feel the jar with few milliliters of water and run the BLAM for a few minutes in MPA mode As clean water 1s atomized it washes the cavities inside the nozzle and the jet holes WARNING Whether you are using the BLAM in SPA or MPA mode always replace the internal liquid aspiration feeding tube during the cleaning decontamination process when you switch from one test article to another to avoid potential cross contamination Autoclaving can be used to decontaminate the BLAM parts if the device is used with pathogenic test articles If you opt for autoclaving you should first contact CH Technologies in order to establish appropriate guidelines for cleaning under high temperature and pressure is not carried out properly it may cause mechanical damage and or WARNING If autoclaving or decontamination with corrosive chemicals chemical corrosion to various BLAM parts The O ring in the BLAM s lid should be inspected during each cleaning The slightest tear or cut on the O ring will result in an inappropriate sealing of the device and may result in leaking of the test article into the air The O ring should be changed at frequent intervals because it may deteriorate with time 2 5 Nozzle Maintenance and Adjustment To make sure that the BLAM performs cons
3. Blaustein Atomizer BLAM Multi jet Model CH TECHNOLOGIES USA CONTENTS 1 General Description and Specifications oven e cece nnn eeen eenen 3 ET MAURO CIC tio Bono masas 3 12 SPECI CAUONS ts nae 4 1 3 Mechanism ODS TON usse te comis RR RD PED EN I DES 4 2 BLAM 6 Dal Packs the BLAM zen ee 6 Tesuns m 6 2 9 BLAM Operation Procedures ES epe mda ue ex eat URS er bl 7 2 3 1 Operating BLAM in MPA Mode eenen 7 2 3 2 Operating the BLAM in MPA Mode with External Refill 8 2 3 3 Operating the BLAM in SPA 10 2 4 Cleaning Decontamination Procedures ane eeens 11 2 5 Nozzle Maintenance and eneen 12 GED TT Douce nodis mere ne see ee 14 NISL are 15 Appendix 1 Overview of Multi jet BLAM Assembly in MPA Mode 16 Appendix 2 Overview of Multi jet BLAM Assembly in SPA Mode 17 Appendix 3 BLAM Exploded Das am aan ae a YR EE 18 Appendix 4 View of Multi jet BLAM esses 19 Appendix 5 View of the Complete Multi Jet BLAM Kat 20 1 GENERAL DESCRIPTION AND SPECIFICATIONS 1 1 Introduction The Blaustein Atomizer BLAM is a high output atomizer that uses t
4. ane tubing or one that that can withstand the pressure used BLAM s air inlet is equipped with a 1 4 push to connect fitting so the air line can be connected by simply pushing the tubing in If you BLAM comes with non standard fittings use appropriate couplers to connect the air line to the atomizer If you are not using any of CH Technologies system control units make sure that the compressed air line 1s equipped with appropriate pressure and flow controllers An illustration of a standard setup of the BLAM in MPA mode is given in Figure 2 5 Connect the BLAM outlet to your system or application using appropriate fittings Make allowance for dilution of the aerosol outflow 1f desired 6 Start atomization by turning on air 7 Using your flow controller adjust the air flow at about 2 L min per jet 8 L min for the 4 jet BLAM 16 L min for the 8 jet BLAM If you need more output increase first the upstream pressure of the compressed air line and then increase air flow rate to the atomizer ATTENTION DO NOT exceed 90 psi upstream pressure 8 Turn off atomization at any time by shutting down compressed air supply to the atomizer 9 Remove the compressed air line from the BLAM s air inlet 10 Take the atomizer for cleaning decontamination Aerosol VALVE PRESSURE T REGULATOR FLOWMETER Compressed Air Pd PRESSURE GAUGE GE BLAM x ATOMIZER Figure 2 Schematic of BLAM s
5. ble it into the lid Make sure that the assembly is air tight ATTENTION DO NOT fill the jar excessively The solution in it serves only the purpose of a soft impaction surface for the aerosol and it will not be used for atomization If you will use a syringe pump for feeding the BLAM fill the syringe with the predetermined amount of test article and place it in the pump Use a syringe large enough so that the BLAM does not run out of liquid before the atomization session IS up If you are using a low flow peristaltic pump instead fill a glass beaker or another appropriate container with the predetermined amount of liquid and dip the inlet end of the pump s tube in it If your test article is a solution containing pathogens make sure that the container is properly sealed with a ventilation hole on the lid Using a piece of thin silicon tube connect the outlet of the syringe or low flow peristaltic pump to the BLAM s liquid feed port 10 ATTENTION Make sure that all the connections between the liquid feed pump and the BLAM are air tight 10 Turn ON the liquid feed pump and adjust the feed rate as desired 11 When the liquid reaches the liquid feed port of the BLAM turn ON compressed air supply to the device 12 Next follow steps through 7 in Section 2 3 1 to operate the atomizer 13 When the atomization process 15 up turn OFF the liquid feed pump and in quick sequence shut down compressed air suppl
6. d or autoclaved 13 3 DISCLAIMER The BLAM Atomizer is subject to all the terms of CH Technologies USA standard limited warranty The BLAM 1s a specialized article of research equipment intended for use by trained professionals No liability is accepted by CH Technologies USA on account of misuse misapplication or mishandling CH Technologies USA specifically does not condone nor endorse any application or use which involves restricting emissions from the device and thus causing pressurizing of the jar Further CH Technologies USA accepts no liability for exposure to toxic infectious or harmful substances resulting from the use of this apparatus 14 4 LIMITED WARRANTY CH Technologies USA warrants equipment of its manufacture to be free from defects in workmanship and material We make no warranty express or implied except as set forth herein CH Technologies USA s liability under this warranty extends for a period of one 1 year from the date of shipment It is expressly limited to repairing or replacing at the factory during this period and at CH Technologies USA s option any device or part which shall within one year of delivery to the original purchaser be returned to the factory transportation prepaid and which on examination shall in fact be proved defecting CH Technologies USA assumes no liability for consequential damages of any kind The purchaser by acceptance of this equipment shall assume all liabil
7. e The BLAM assembly as seen in Appendix 1 or 2 e Package of accessories containing 2 spare 10 32 barb fittings 2 spare 10 32 plugs 2 spare O ring sets Spare silicon tubing for liquid feed Allen wrench for the nozzle screws e Carrying Case Your BLAM may vary slightly in appearance depending upon the specific model that you ordered 4 jet vs 8 jet type of discharge vertical vs horizontal and fittings you requested Appendix 4 shows photographs of multi jet BLAMs with vertical and horizontal discharge An overview of parts and accessories of the BLAM s complete kit is given in Appendix 5 Any changes from the standard pieces listed above will be noted on your shipping memo PLEASE KEEP YOUR SHIPPING MEMO FOR FUTURE REFERENCE If you find that anything has been damaged in transit contact the carrier of the shipment immediately in addition to CH Technologies 2 2 Initial Testing After unpacking you should immediately check the operation of the device Your BLAM comes fully assembled so it ready to be used for a performance check Follow the steps outlined in Section 2 3 1 to set up and operate the device Use ONLY distilled water for initial testing When the air source is turned on mist should appear at the aerosol outlet as shown in Figure 2 If mist does not appear recheck all your fittings and make sure that jar is screwed in tight The operation of the BLAM depends upon it being a completely sealed unit Perfor
8. he jet nebulization principle of the Collison Nebulizer which has long been the recognized as the technique for the efficient aerosolization of various liquids However the BLAM relies on a new patent pending design which allows for a more efficient generation of aerosol than the Collison or other existing devices in both its single pass and recirculating configurations This innovative design allows users to produce liquid aerosol at high particle concentrations and very narrow particle size distribution The BLAM is superbly suited to handle various liquid media such as water based drug solutions solvent based mixtures such as pesticides oils and other viscous liquid suspensions and biological test articles such as bacterial viral suspensions The multi jet BLAM 1s able to efficiently aerosolize and deliver high aerosol outputs of viscous liquid media which is particularly of great importance in meeting the OECD requirements for pesticide testing Also it provides bioaerosols with enhanced viability of pathogenic microorganisms for aerobiology research The particle size distribution of the aerosolized mist will depend on the nature of the liquid test article However for most solutions and suspensions the BLAM will deliver a narrowly distributed mist that falls well within the respirable cutoff The multi jet BLAM can be used in Single Pass Atomization SPA mode with the liquid feed being injected externally by a metering device or Mu
9. istently over long periods of time the nozzle should be serviced periodically The frequency of the service will depend on the usage intensity and type of test articles utilized The maintenance procedure of the nozzle is the following 1 Remove the nozzle from the air stem using appropriate wrenches 12 Remove the barb and plug fittings from the liquid feed aspiration ports Use the provided allen wrench to remove the four screws that hold together the nozzle plates Split the plates and lay them down on a clean work surface Remove and carefully inspect the O rings that seal the plates against each other refer to Figure 1 for details If they show signs of damage replace them with O rings from the spare set supplied with the BLAM Visually inspect the jet holes against a light source and observe if there is any visible occlusion present in any of them If any of the jets is clogged place the jet plate in an ultrasonic bath with distilled water for about 30 minutes Wash the plates with a mild soap solutions and rinse them well with tap or distilled water After the plates have dried place the O rings on the plates and reassemble the nozzle Apply some Teflon tape on the threads of the air stem and thread the nozzle using appropriate wrenches any servicing of the nozzle should take place after the device has been WARNING If the BLAM has been used with pathogenic test articles properly decontaminate
10. ity for consequences of its misuse by the purchaser his employees or others This warranty will be void if the equipment is not handled installed or operated in accordance with our instructions If damage occurs during transportation to the purchaser CH Technologies USA must be notified immediately upon arrival of the equipment A defective part in the meaning of this warranty shall not when such part is capable of being repaired or replaced constitute a reason for considering the complete equipment defective Acknowledgment and approval must be received from CH Technologies USA prior to returning parts or equipment for credit No representative of ours has authority to change or modify this warranty in any respect 15 Appendix 1 Overview of Multi jet BLAM Assembly in MPA Mode Jaeger Joint Air Inlet Fitting N Outlet Air Stem X Compression Nut N Plug N O ring Lid us Plug Atomization Nozzle Liquid Aspiration Tube Precious Fluid Well 16 Appendix 2 Overview of Multi jet BLAM Assembly in SPA Mode Jaeger Joint Air Inlet Fitting CX Outlet Air Stem N 1 Compression Nut Liquid Feed N f Port O ring Liquid Feed Tube Polycarbonate Jar Atomization Nozzle Precious Fluid Well Plug 17 Appendix 3 BLAM Exploded Diagram gu Air Inlet Fitting p i Air Stem m Compression Nut ais Be ER Ferrules Lid With Aerosol Outlet HB Liquid Feed B
11. liquid feed port located on the Body The aerosol generated by the atomization process is sprayed downwards and after impacting the surface of the liquid inside the jar it makes a 180 turn and it 15 forced up the outlet tube on top of the BLAM lid During this process larger droplets are collected on the liquid surface due to impaction as they cannot make the U turn The end result 1s a very fine mist well within the respirable range and with narrow size distribution The properties of the aerosol generated by the multi jet BLAM are a function of the jet hole size and expansion cone size The BLAM jets are equipped with 0 010 diameter laser cut rubies which guarantee long lasting precision in the atomization process For specific applications the BLAM can be fitted with other non standard jet hole sizes and expansion plates The multi jet BLAM is equipped with a precious fluids jar which has a small well at the bottom In MPA mode the thin silicone tube attached to the liquid aspiration port on the Expansion Plate extends all the way to the bottom of the well This allows the atomizer to essentially use every last drop of the test fluid See Appendices 1 and 2 for an overview of parts of an assembled multi jet BLAM in both SPA and MPA mode 2 BLAM OPERATION 2 1 Unpacking the BLAM When you receive your BLAM you should unpack it carefully and check to make certain that the following parts are all present and in undamaged condition
12. lti Pass Atomization MPA mode where the atomizer jar is pre filled with the liquid test article and the medium is cyclically re circulated atomized to the four or eight sonic velocity air jets until is exhausted The BLAM as a retrofit can be exchanged for existing Collison type nebulizing nozzles and used as an atomizer by simply mounting it in a suitable inhalation exposure system using standard tube and pipe fittings The retrofit kit is packaged as a direct nozzle replacement for many USA Collison nebulizers The BLAM is equipped with a vapor polished polycarbonate jar that is highly resistant to heat and mechanical stress but at the same time allows a clear visual of the atomization process inside the device As all other CH Technologies nebulizers the BLAM outlet 15 equipped with a vertical or horizontal Jaeger joint connector that is the standard connector of our inhalation systems Other custom designed connectors are available upon request The complete CHT BLAM is best used as an atomizer that interfaces directly to CH Technologies systems and equipment but with suitable modifications also adapts to other non CHT equipment 1 2 Specifications Particle diameter Approximately 0 7 2 5 um Models available 4 and 8 jet Air flow rate 4 30 L min Liquid Feed Rate 0 1 60 ml min Discharge Vertical or Horizontal Material Stainless Steel 316 stem amp plates and Ploycarbonate jar amp head 1 3 Mechanism of Operatio
13. m a visual inspection of the jet operation and make sure that none of them appears to be aerosolizing less than others or not at all All the jets are identical and should perform exactly the same 2 3 BLAM Operation Procedures 2 3 1 Operating the BLAM in MPA Mode Your BLAM comes set up for MPA mode operation In this mode the liquid feed hole on the lid as well as liquid feed port on the nozzle body are plugged A barb fitting with a short piece of silicon tube is assembled into the liquid aspiration port on the jet plate See Appendix 1 for a representation of the BLAM in MPA mode Follow these steps to operate the BLAM in MPA mode 1 Remove the jar and fill it with about 100 ml of the test solution or suspension device is operated at high outputs and or for long aerosolization sessions ATTENTION The amount of liquid can alternatively be greater if the or smaller af you are using precious fluids 2 Reassemble the jar into the lid and make sure that it s tight 3 Release the compression nut that holds the air stem and adjust the height of the nozzle at about 1 from the surface of the liquid Make sure that liquid aspiration tube connected to the nozzle is long enough to reach to the bottom of the precious fluid well Tighten the nut when you are done ATTENTION NOT use tools to tighten the air stem compression nut 4 Using a 4 OD tube connect a compressed air line to the air inlet of the BLAM Use polyureth
14. n Knowledge of the mechanism of aerosol formation by the BLAM will help you in determining the aerosol characteristics as well as being useful for maintenance The atomizing head at the end of the air supply stem is the key to aerosol formation It is composed of three main parts Nozzle Body Jet Plate and Expansion Plate Figure 1 Compressed Air Nozzle Body Liquid Channel Liquid Feed Port N Air Channel O ring Assembly Screw N x Jet Plate Jet Hole Jet Ruby Expansion Cone Liquid Cavity Liquid Suction Port Expansion Plate Figure 1 Components of BLAM s Atomizing Nozzle The atomization occurs when the pressurized air coming from the stem pushes through the jets on the Jet Plate at sonic velocity The jets are very small orifices through which pressurized air flows at a constant velocity This process creates a powerful vacuum effect in the cavity only 0 002 deep between the Jet Plate and Expansion Plate which drives the liquid from the liquid chamber to the jets The liquid then reaches the wells around the jets where is aspirated in and sheared into tiny droplets as the air expands after 4 the jet In multi pass mode the liquid is driven into the liquid chamber through the suction tube at the center of the Expansion Plate simply by the vacuum created by the air Jets On the other hand in single pass mode the suction port on the Expansion Plate is plugged and the liquid is pumped in through the
15. re done with the atomization process Remove the refill tube from the liquid feed port on the lid Remove the compressed air line from the BLAM s air inlet Disconnect the atomizer from the external refill pump and take it for cleaning decontamination Aerosol VALVE PRESSURE REGULATOR FLOWMETER r r Compressed Air Liquid Feed o p ER A PRESSURE GAUGE BLAM ATOMIZER Figure 3 Schematic of BLAM s Setup in MPA Mode with External Refill 2 3 3 Operating the BLAM in SPA Mode When you are using your BLAM with live pathogen suspensions it is recommended that the device be operated in SPA mode In this mode the test article passes only once through the atomization process which results in preservation at a large extent of the viability of the microorganisms in the aerosol The SPA mode is also recommended for proteinic solutions because prevents foaming during atomization Follow these steps to convert the BLAM and run it in SPA mode 1 2 Remove the BLAM jar Remove the liquid feed port plug on the lid Install the provided liquid feed barb instead of it and tighten it up Switch places of the liquid aspiration port barb fitting center of the jet plate with the liquid feed port plug on the nozzle body Using a piece of the supplied silicon tube connect the liquid feed port on the lid with the liquid feed port on the nozzle body Fill the jar with about 20 30 ml of test solution and reassem
16. ulkhead Wes Lis Feed Barb mier BLAM Nozzle Body BLAM Expansion Plate Oring Shoulder Bolt x4 iib o Polycarbonate Precious Fluids Jar 18 Appendix 4 View of Multi jet BLAM Vertical Discharge BLAM Horizontal Discharge BLAM 19 Appendix 5 View of the Complete Multi Jet BLAM Kit O ring Set O ring Set E a OO OO MM Barbs Silicon Tube Spare Plugs Allen Key w 4 Complete BLAM Assembly 20
17. y to the atomizer 14 Remove the liquid feed tube from the liquid feed port on the lid 15 Remove the compressed air line from the BLAM s air inlet 16 Take the atomizer for cleaning decontamination Aerosol VALVE PRESSURE REGULATOR FLOWMETER Air plc v DE Frauen Liquid Feed _ PRESSURE GAUGE SYRINGE PUMP HE er en BLAM ATOMIZER Figure 4 Schematic of BLAM s Setup in SPA Mode 2 4 Cleaning Decontaminating Procedures Your BLAM should perform indefinitely if it 15 cleaned and maintained properly The device should be cleaned after each use 11 If you are using your BLAM with any aqueous solutions that contain no pathogens cleaning with a mild soap solution in warm water is generally adequate Depending on the test article used special solvents may be used in certain cases Be sure that the O rings are compatible with any solvents used Special chemical resistant O rings are available from CH Technologies if needed Distilled water is a good choice for the final rinse warm tap water may work also You must judge the success of your cleaning rinsing with your own QA QC wipe tests or similar assessments The cleaned BLAM parts should be allowed to air dry or they may be dried with warm forced air If your tests involve atomization of viscous suspensions or you are switching from one test article to another an additional step is needed to make sure that the jets are freed from any
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