User ManUal s 9432 HPlC PUMP systeM
Contents
1. After the pump reached its flow rate it automatically goes to HOLD this means the pump is running but the gradient program time is on hold The pump will switch from HOLD to RUN after pressing the START key again or by external signal 37 38 The current state of the gradient program can be checked on the Status Screen be pressing the STATUS key Mode Intern Status Run Prog Mode Intern Status Run Prog Flow 1 0005 Pres 32bar The bottom line shows the current gradient composition while the center of the status screen shows the current step step time and total time 6 4 2 Stopping the Pump The S 9432 HPLC Pump System can be stopped by pressing the STOP key The pump will stop immediately unless a Stop Ramp is set Flow Stop Ramp If a time is set for the Stop Ramp the flow rate will slowly decrease from the selected flow rate to 0 over a selected time e g Stop Ramp time Maximum Pressure In case the Maximum Pressure threshold is met the pump stops immedi ately Minimum Pressure In case the Minimum Pressure threshold is met the pump stops ifthe pres sure stays below the threshold for 30 seconds 7 INSTRUMENT MAINTENA 7 1 Pumphead 7 1 1 Pumphead Assembly The following diagram shows all the components of the dismantled pump head 16 ye i s lt lt N 10 q a u 9 7 8 i v y1 PN ES i 9 UN y DE 2s E i Y EN el 5 y IDAS q e Pr yo IN A a 6 22. Hold Timeout The Hold Timeout specifies a timeout after which the pump automatically stops when it is currently in Hold If the Timout is set to 0 this feature is disabled Delay Time The Delay Time specifies a delay between starting the pump and the pump actually starting Start Ramp The Start Ramp defines a time in which the flow slowly ramps up when started Stop Ramp The Stop Ramp defines a time in which the flow slowly ramps down when stopped 29 30 5 3 3 Submenu Pump Menu gt Calibration Network Address The Network address can be any number between 0 and 15 It is used for specific cases of external control mode Head The Head settings defines the installed pump head Head Constant The Head Constant is a setting for an individual pump s flow rate adjust ment Do not change this value Pressure Comp The Pressure Compensation is a setting for an individual pump pressure compensation adjustment Do not change this value Pressure Zero The Pressure Zero is an offset for the installed pressure sensor Only change this value when the pressure sensor is changed 5 3 4 Submenu Gradient Menu Prog Run Select the Program Number 1 8 which should be run Prog Edit Select the Program Number 1 8 to edit see below Enter Gradient In this menu the Gradient Program can be edited The Program Number is selected in the Menu above Editing Values Each value o
3. of Channels Degassing Technique Wetted Materials Volume per Channel Inner Diameter Capillary Degassing Efficiency 1 2 3 or 4 Applied vacuum through semiperme able membrane Teflon AF Stainless Steel PEEK lt 500 ul 1 1mm lt 20 dissolved gases remaining in water at 1 0 ml min B 1 2 Vacuum Pump optional Mechanism Stepper Motor driven membrane pump Wetted Material Fumes only Teflon Aluminium EPDM B 1 3 Pump Wetted Materials Flow Rate Flow Accuracy Flow Precision Gradient Mixing Pressure Range Pressure Pulsation Dimensions Weight Power Supply Stainless Steel PEEK PVDE Ceramics Ruby Micro 0 001 4 000 ml min Analytical 0 001 10 000 ml min Semi Preparative 0 001 40 000 ml min 1 0 1 000 ml min 0 1 RSD 1 000 ml min Dynamic 516 923 ul adjustable mixing volume 0 40 MPa 0 6000 PSI typical lt 0 1 MPa or lt 1 0 310 x 165 x 478 mm W x Hx D 7 0 kg 100 250 V 47 63 Hz 20 W depending on material option 45 B 2 Environmental Conditions B 2 1 Operational Conditions Ambient Temperature 10 C to 35 C Ambient Relative Humidity 20 to 80 RH non condensing B 2 2 Storage Conditions Ambient Temperature 20 C to 60 C Ambient Relative Humidity 20 to 80 RH non condensing 46 APPENDIX C ACCESSORIE C 1 Standard Accessories The S 9432 HPLC Pump System is delivered with t
4. 7 Instrument Maintenance 7 1 Pumphead 7 1 1 Pumphead Assembly 7 1 2 Piston Backflushing 7 1 3 Change of Piston Ring 7 1 4 Replacing Check Valves Appendix A Compressibility Appendix B Specifications B 1 Technical Specifications B 1 1 Vacuum Chamber optional B 1 2 Vacuum Pump optional B 1 3 Pump B 2 Environmental Conditions B 2 1 Operational Conditions B 2 2 Storage Conditions Appendix C Accessories C 1 Standard Accessories Isocratic Version Appendix D Version Control D 1 Version History 31 32 33 35 35 35 35 35 35 37 37 38 39 39 39 40 40 40 43 45 45 45 45 45 46 46 46 47 47 47 49 49 1 INTRODUCTION 1 1 How to Use this Manual This manual is designed as a reference to the installation operation and maintenance of the S 9432 HPLC Pump System It is strongly recommended to review this manual before operating the in strument The content of this manual is subject to change without notice This docu ment is believed to be complete and accurate at the time of publication The Manufacturer is not liable for any damage resulted from the use of this manual 1 1 1 Symbols Throughout this manual important text sections are marked with the fol lowing symbols This section includes important information for the proper opera tion of the instrument Failure to follow this information may result in faulty behaviour of the instrument and or wrong analys
5. elasticity If the liquid is influenced by pressure changes Ap the volume will change by AV The volume V will become the same as before if the pressure will be reduced to the original value again The dimension of the change in volume is the compressibility This can be shown as relative change of the volume Comme Ao e necessary pressure change therefore 1 AV K x V Ap The constant volume delivery can be seen as a stepwise displacing of liq uid out of the pump head The dimension for the volume delivery is the amount of piston volume during a period of time But this is only correct as long as the delivery is done without any added pressure As soon as the liquid delivery has to be done against a back pressure a specific amount of the piston volume has to be used for compressing the liquid first before the actual delivery can start This can be described by the following diagram Y Element p D c suction pressure delivery volume delivery pressure p A l v suction of liquid during pressure p1 compressing the liquid from p1 to p2 delivery against pressure p2 changing from delivery to suction 43 44 The compressibility will effect the constant of the liquid delivery depending on the pressure To overcome this problem the S 9432 is using a calcula tion program to correct the pressure depending change of the flow rate This calculation program corrects the com
6. selected mode Intern Extern or Serial Status On the top right the current status is displayed Stop Hold Run or Error Flow The Flow Rate is shown on the left center area It always shows the current Flow Rate Pressure The current pressure is shown in the right center area The pressure is shown in the selected unit e g Bar MPa or PSI 5 3 Menu Description With the MENU key the Main Menu is displayed Use the CURSOR key UP and DOWN to browse through the Main Menu for access of one of the Submenus 27 28 5 3 1 Submenu Pump Menu gt gt Pump Settings Min Pressure The Minimum Pressure threshold The pump will stop if the pressure falls below this threshold Max Pressure The Maximum Pressure threshold The pump will stop immediately if the pressure rises above this threshold Max Pr Purge The Maximum Purge Pressure threshold The pump will stop immediately if the pressure rises above this threshold when the Purge function is used This a safety feature to prevent column damage when the purge valve is closed while Purge is active Change Unit The Pressure Unit can be changed here Available selections are Bar MPA and PSI All pressure values are shown in this selected pressure units Compressibility The Compressibility can be changed here A Compressibility of 1 000 is used for water and 0 700 for Methanol 5 3 2 Submenu Pump Menu gt Run Parameters
7. the front to make capillary connections or change pump sealings The Purge Valves 8 10 is used for the priming of the pumps 3 2 2 Functional Elements All functional elements of the 9432 HPLC Pump System are located inside the instrument The Display PCB 1 controls the TFT Display and Keyboard It is acces sible when the display panel is removed The Controlled PCB 2 controls the whole pump The Power Supply Unit 3 supplies 24V to the PCB s and pump motor The input voltage is variable from 90 V to 240 V The Vacuum Pump 4 is mounted on a drain tray It is connected to the Vacuum Chambers 5 and the gas outlet on the back panel The Vacuum Chambers 5 are mounted on a drain tray and are connected to the vacuum pump and the Controller PCB The Mixing Chamber 6 is mounted on a drain tray and connected to Sol vent Inlet Block on the front panel and the Vacuum Chambers 5 The Pump Mechanic 7 drives the pumphead and includes the camshaft lubrication chamber driving pistons and the stepper motor Element 6 Mixing Chamber e Pump Mechanica 17 4 INSTRUMENT SETUP 4 1 Unpacking Remove the S 9432 HPLC Pump System from its package and put it on the working desk Check the instrument thoroughly for any damage that may have occurred during shipping Contact your supplier in case of any damages Check the accessories shipped with the instrument if ev
8. 3 6 INSTRUMENT OPERATION 6 1 Overview The S 9432 HPLC Pump System can be operated in 3 different Operation Modes Internal External or Serial 6 1 1 Internal Mode In Internal Mode the instrument is controlled manually with the Keyboard 6 1 2 External Mode In External Mode the instrument is controlled via the Remote Digital I O contacts 6 1 3 Serial Mode In Serial Mode the instrument is controlled by a PC software e g Clarity via RS 232 serial line 6 2 Priming the Pump Before the S 9432 HPLC Pump System can be operated it must be primed with the required mobile phase e connect the plastic syringe to the purge valve outlet e suck the eluent through the tubing till it starts filling the syringe keep the syringe on the purge valve outlet e press PURGE and check if the syringe is filling with constant speed Now the pump is primed and ready for operation 35 6 3 Operating the Pump in Manual Mode 6 3 1 Starting the Pump The S 9432 HPLC Pump System can be started by pressing the START key The pump will run at the selected flow rate Flow Start Ramp If a time is set for the Start Ramp the flow rate will slowly increase from 0 to the selected flow rate over a selected time e g Start Ramp time This feature gently increases flow and therefore pressure and may increase the lifetime of some specific columns Mode Intern Status HOLD Mode Intern Status Run Prog Pressure
9. 3 e A ot Element Element Outlet valve housing pressure Piston guide with piston back side flushing Capillary tube for connecting the damping piston 4 Secondary sealing ring Hollow screws M4x45 1 1 6 a 5 4 5 4 ressure spring Ceramic piston assembly 8 Relief valve block 0 Sealing ring relief valve 7 12 Inlet valve housing suction side 13 Ferrule 1 8 1 1 2 Capillary for connecting relief valve and pump head Drain capillary from relief valve block Solvent outlet EN EM 7 Eu D g 5 22 23 24 39 7 1 2 Piston Backflushing When using saliferous eluents a growth of salt crystals is possible behind the piston ring on the ceramic piston Under unfavorable working condi tions these salt crystals can lead to a higher wearing of the piston ring Generally it is sufficient to rinse the rinsing chamber between piston ring and secondary sealing ring with distilled water once a week The water remaining in the rinsing chamber stops the crystallization of salt crystals Therefore the lower capillary tubes should be connected together with PTFE tubing so that the water remains in the rinsing chamber The rinsing of the chamber should be done with the plastic syringe deliv ered with the instrument 7 1 3 Change of Piston Ring e Loosen the two hollow screws 10 for removing the mounting plate 9 e take off the guide bearing piston assembly pr
10. Chambers used in the Quaternary Gradient Module are low volume high efficiency chambers using the advanced Teflon AF material The inner diameter and wall strength of the Teflon AF capillary used in these chambers is optimized for best efficiency with low volume Teflon AF is as chemical compatible as normal Teflon but has a much higher gas diffusion rate making a capillary 1000 times more efficient Vacuum Pump Vacuum Chambers The Gradient Module features an The Gradient Module s 2 vacuum integrated vacuum pump for online chambers are fitted with high ef degassing The advanced Teflon lt ficiency Teflon AF tubings for low membrane guarantees a long volume and increased degassing lifetime and maximum chemical efficiency compatibility Secure Assembly Active Mixer The Gradient Module incorporates an active mixer with adjustable vol ume for optimized gradient mixing The Gradient Module is assembled on a stainless steel tray with a separate drain to protect the instru ment in case of leakages 11 Vacuum Degasser Working Principle The solvent flows through a short length of Teflon AF capillary inside a sealed chamber This chamber vacuum chamber is completely sealed to the environment and vacuum is applied with a pump This vacuum pump decreases the pressure inside the chamber to near vacuum Due to this vacuum any dissolved gases in the solvent running through the inner capillary are removed throu
11. S 9432 HPLC PUMP SYSTEM HIGH PRESSURE GRADIENT SYSTEM USER MANUAL VERSION 1 0 2014 05 26 TABLE OF CONTENTS Contents 1 Introduction 1 1 How to Use this Manual 1 1 1 Symbols 1 2 Safety Information 1 2 1 General Safety Information 1 2 2 Intended Purpose 1 2 3 Environmental Safety 1 2 4 Electrical Safety 2 Instrument Overview 2 1 General Instrument Overview 2 1 1 Pump Mechanic 2 1 2 Gradient Module 3 Instrument Description 3 1 General Instrument Description 3 1 1 Front Panel Description 3 1 2 Back Panel Description 3 2 Functional Description 3 2 1 Front Elements 3 2 2 Functional Elements 4 Instrument Setup 4 1 Unpacking 4 2 Capillary Connections 4 3 Backside Connections 4 3 1 Drain Gas Exhaust Connections 4 3 2 Electrical Connections 4 3 3 Remote Connector 5 Instrument Menus 5 1 Keyboard 5 2 Status Screen 5 3 Menu Description 5 3 1 Submenu Pump Menu gt Pump Settings 5 3 2 Submenu Pump Menu gt Run Parameters 5 3 3 Submenu Pump Menu gt Calibration OO ananannuu wo W Zo hon nw WwW N e pu euouaa unn ons DON a u UI vunn N O ooN 5 3 4 Submenu Gradient Menu 5 3 5 Submenu Degaser Settings 5 3 6 Submenu Systemmenu 6 Instrument Operation 6 1 Overview 6 1 1 Internal Mode 6 1 2 External Mode 6 1 3 Serial Mode 6 2 Priming the Pump 6 3 Operating the Pump in Manual Mode 6 3 1 Starting the Pump 6 4 2 Stopping the Pump
12. erything is com plete and in good condition 19 4 2 Capillary Connections The next step is to make all fluid connections The following list describes all necessary steps to make the proper capillary connections With Degasser Option e Connect the 1 8 PVDF tubing to the Solvent Inlets 2 e Connect the 1 8 PVDF tubing from the Solvent Outlets 3 to the Pumphead Inlets 5 and 6 e Fix the 1 8 capillaries with the Capillary Holder 1 Without Degasser Option e Connect the 1 8 PVDF tubing to the Pumphead Inlets 5 and 6 Fix the 1 8 capillaries with the Capillary Holder 1 e Connect a 1 16 capillary Micro Analytical I D 0 5mm Semi Preparatibe I D 0 75mm to the Pump Outlet 4 this capillary is connected to the autosampler or manual injection valve Element Pump B Inlet lo Pump A Inlet AR WARNING Make sure that the eluent bottles are on a higher level than the S 9432 so that the solvents can freely flow downwards 20 4 3 Backside Connections 4 3 1 Drain Gas Exhaust Connections The Drain Outlet is only connected with the optional Vacuum Degaser e Connect the supplied gas tubing blue colored to the GAS OUT connector on the backside of the instrument e Connect the supplied silica tubing to the DRAIN outlet on the back When working with aggressive and or toxic solvents make sure that any fumes from the GAS OUT connector is properly disposed of and not evap
13. essure springs and centering discs e pull out the piston guide e pull out the piston rings very carefully with the help of a pair of tweezers e completely insert the new sealing rings into the opening flange up ward the small spring has to show in direction of the pump head e insert the piston guide upward with the secondary sealing ring white put the ceramic piston into the guide bearing insert the spring with the centering discs in the piston and place the whole unit on the piston guide put the mounting plate on top and fasten it with the hollow screws 10 e completely push down both pistons by hand for several times in order to check if the pistons were jammed during the process of as sembling 7 1 4 Replacing Check Valves The check valves are constructed as cylindrical cartridges The cartridges have sealing rings made of PEEK mounted on both sides These sealing rings have to ensure that the solvent will not bypass the check valve Both valves on the high and low pressure side of the pump are iden tical and therefore can be used for both sides Place the cartridge in the valve housing and fasten first by hand and then turn another half of a turn by using the 13mm wrench delivered with the instrument If the pump is not delivering the correct flow rate the valve housing might not be tightened enough Before tighter fasting make sure that the pump was carefully flushed Cleaning the Check Valve Cartr
14. f a Gradient Program line can be edited with the EDIT key and the UP and DOWN keys Confirm the change with EDIT and cancel the change with CENCEL key Adding Lines A new line can be added by pressing the EDIT key when the cursor is in the first column Deleting Lines A line can be deleted by pressing the CANCEL key when the cursor is in the first column 31 32 5 3 5 Submenu Degaser Settings Degaser Select lt ON gt or lt OFF gt to switch the degaser on or off Mode Select the degasser mode HYSTERESIS mode switches the dgeasser on and off depending on the vacuum level In CONTINUOUS mode the vac uum pump runs constantly Speed The speed of the vacuum can be selected to be either HIGH or LOW This speed setting has no impact on the degassing performance just on the time needed to reach the vacuum level Vacuum Level The current vacuum level is displayed 950 vacuum chamber pressure 5 3 6 Submenu Systemmenu Int Ext Ser The Operation Mode can be changed here between Intern Extern or Serial Software Display The Software Display is the installed firmware version of the Display PCB Software Mainb The Software Display is the installed firmware version of the Controller PCB 24V Voltage The 24V Voltage is an actual voltage reading for one of the PCB s controlled voltages Runtime The runtime shows the total runtime of the pump 3
15. gh its semi permeable membrane wall The high efficiency of the Teflon AF material allows the usage of a very short length of capillary inside the vacuum chamber This low dead volume offers a quick solvent exchange and very short equilibration time lt Inlet Near Vacuum gt 0 co Vacuum Sensor Vacuum Pump 12 3 INSTRUMENT DESCRIPTION 3 1 General Instrument Description 3 1 1 Front Panel Description The front panel of the S 9432 HPLC Pump System consists of three main elements the TFT Display 1 the Keyboard 2 and the Front Cover 3 The 3 TFT Display shows the current status information and is used with the Keyboard to adjust instrument settings and fully operate the instru ment in stand alone mode without a PC required The Front Cover has a view panel so that the important parts Pumphead and Purge Valve are always visible to the operator In case of a leak it can be detected before solvents are leaking out of the bottom of the Front Cover The Front Cover can be opened to easily access the Pumphead Purge Valve and capillary connections Element 13 14 Removing the Front Cover The Front Cover of the S 9432 HPLC Pump System is completely remov able at its hinges Open The Front Cover to 90 in order to get free of any instrument stacked on top Remove the Front Cover Store the Front Cover at a safe place 4 3 1 2 Back Panel Descrip
16. he following standard accessories e 1x Power Cord EU Type e lx Serial Cable RS 232 e 2x 1 8 Capillary FEP 2 m e 2x 1 8 Fitting amp Ferrule PVDF e 1x 1 16 Capillary I D 0 50mm 60 cm SS or PEEK e 1x Wrench 12x 13mm e 1x Wrench 1 4 x 5 16 e 1x Allen Wrench 2 5mm e 1x Allen Wrench 3 0mm e 1x Plastic Syringe w Tubing Adapter e 1x Operation Manual this e 2x Fuse 2 A 47 APPENDIX D VERSION GON D 1 Version History The 9432 HPLC Pump System Operation Manual is subject to version con trol The version history is continued and noted on every document The access to the original document is restricted to the creating party The doc ument is subject to periodical checks and can never reach an unchangeable state Version Release Date Description 1 0 2014 05 26 First Release 49
17. idge Generally the check valves cartridges don t wear However a deposit of dirt in the valve can influence the function In this case only a limited improve ment will be achieved when cleaning the fully assembled cartridge The more dependable method is to dismantle the valve for cleaning In order to avoid any loss of valve parts it is recommended to use a small container for the process is dismantling e pull out both sealing discs 1 e carefully push out the valve contents 3 5 e clean the valve part depending on the degree of dirt either with a washing bottle or with an ultrasonic bath e insert the sealing disc which is next to the ring marker e then insert the sapphire seat with the polished side toward the ruby ball e place the ruby ball in the deeper side of the check guide 5 and put both into the bearing 2 with the ruby ball 4 facing the sapphire seat 3 e press the second sealing disc on top After assembling check the functioning of the valve by blowing air through it It is possible to blow the air from this side of the ring marker through the valve and it should lock when blowing the other direction Vila IH O O 41 Element 1 PEEK sealing ring marker 5 Check guide made of ceramic 42 APPENDIX A COMPRESSIB A general problem for constant flow delivery with piston pumps is the compressibility of the solvents under pressure Any liquid shows a specific volume
18. is constantly lubricated within a sealed chamber to guarantee long lifetime and low maintenance Stepper Motor The stepper motor driven camshaft driving the pis tons is optimized for the typical analytical flow R sonar a range especially in the Piston Backflushing lower flow rate regions z en 8 The S 9432 s pump head in 4 corporates an automatic piston backflushing system this system is interchangeable with old pumps and does not require an additional motor Permanent Lubrication The camshaft and piston drive is permanently lubricat ed within a sealed chamber The special lubrication used makes the S 9432 suitable for 24 7 operation Dual Piston Pumphead The S 9432 pumps use a dual pis ton pumphead for low pulsation Together with electronic pressure compensation the S 9432 pumps are suitable for all analytical tasks in HPLC and GPC Active Piston Backflushing optional The optional piston backflushing actively flushed the piston after the pri mary seals to prevent crystallization of buffer solutions The piston back 10 flushing is incoporporated into the pumphead and does not require any additional pump or motor Because of this all pumps are upgradable to active piston backflushing at any time without downtime Pumphead The short stroke dual piston pumphead is optimized for low pulsation and accurate delivery The 9432 HPLC Pump System can be fitted with any of the fo
19. is results This section emphasis some detailed information intended to optimize the performance of the instrument or to give a better understanding of some technical details NOTE 1 2 Safety Information This instrument is compliant with all related standards as stated in Ap pendix B 1 2 1 General Safety Information The operation of any analytical instrumentation requires the operator to be familiar with the potential hazards of using chemical solvents To avoid personal injury and or damage to the instrument the operator is responsible to follow all safety information herein The manufacturer assumes no liability for any damage resulted from not following any of these safety procedures 1 2 2 Intended Purpose This instrument is designed and certified as a general purpose laboratory instrument for research and routine analysis work only It is not certified for in vitro or other medical applications Any use outside this intended purpose does not fall with the manufactur ers liability 1 2 3 Environmental Safety Only operate the instrument in well ventilated areas If volatile or flam mable solvents are used with this instrument arrange for proper disposal of any waste and or fumes Always properly dispose any waste solvents Avoid open flames and sparks when working with flammable and volatile solvents In case of instrument leakage turn off the instrument and remedy the lea
20. k age problem immediately 1 2 4 Electrical Safety Always use the provided power cords Replace faulty power cords and other cables before operating the instru ment Always replace blown fuses with original spare fuses When the instruments housing is open electrical connections will be ex posed Disconnect the instrument from the main power before opening the housing The housing should only be opened by certified service personnel Damage of the instrument of injury may result from improper han dling 2 INSTRUMENT OVERVIEW 2 1 General Instrument Overview The S 9432 HPLC Pump System is a very flexible and powerful HPLC sol vent delivery system The system is available as Isocratic HPLC pump and Quaternary Low Pressure Gradient Pump with optional integrated online vacuum degasser The Quaternary Low Pressure Gradient version incorporates an active low pressure mixer with adjustable mixing volume The mixing chamber volume can be freely adjusted An optional integrated vacuum degasser removes dissolved gases in the mobile phase and prevents air bubbles in the system 2 1 1 Pump Mechanic The 9432 s pump me chanic is a robust design for long lifetime and minimal maintenance Stepper Motor The S 9432 is driven by a high power stepper motor The stepper motor has a much better resolu tion in the low flow range than a conventional DC motor Lubrication The S 9432 camshaft
21. llowing pumpheads Analytical Pumphead max Flow Rate 10 000 ml min e Micro Pumphead max Flow Rate 4 000 ml min e Semi Preparative Pumphead max Flow Rate 40 000 ml min In addition to the performance types above all pumpheads can be deliv ered in e Stainless Steel e PEEK Titanium on request The different pumpheads should not be exchanged freely on a specific pump as the flow rate is adjusted to a specific type of pumphead When the pumphead is changed without proper flow adjustment the flow rate will not be accurate 2 1 2 Gradient Module The 9432 HPLC Pump System has the optional includes a Gradient Mod ule The whole module is mounted inside the pump and consists of several elements Active Gradient Mixer Optional The active mixer has a variable volume mixing chamber with a magnetic stirrer unit built in The mixing volume can be adjusted freely to optimise mixing performance for different kind of solvents and applications Vacuum Pump Optional The optional online vacuum degasser consists of a Vacuum Pump and four Vacuum Chambers The Vacuum Pump is a high efficiency membrane pump using an advanced Teflon membrane for highest possible chemical compatibility and long lifetime The specific Teflon membrane is devel oped for high frequency deformations to achieve an excellent lifetime of the membrane as chances of ruptures are much reduced Vacuum Chambers Optional The four Vacuum
22. n Function refed 23 5 INSTRUMENT MENUS 5 1 Keyboard The S 9432 HPLC Pump System parameters can be adjusted at any time via the screen menu and keyboard on the front panel Jan a Ca PURGE Ce 1 G MENU Key With the MENU key you can access the Main Menu and select a sub menu STATUS Key With the STATUS key you can access the status screen from any menu EDIT Key Use the EDIT key to change any parameters When you press the EDIT key the selected parameter can be changed Confirm the new setting by pressing the EDIT key again CANCEL Key With the CANCEL key you can abort the change of a parameter When pressing CANCEL key instead of EDIT key when changing a param eter the change is discarded and the original value is used CURSOR Keys The 4 arrow CURSOR keys are used to navigate the menus START Key The START key starts the pump 25 STOP Key The STOP key stops the pump PURGE Key The PURGE key START and STOP together is used to purge the pump Make sure that you open the Purge Valve before pressing the PURGE key When activated the Purge will run the pump with maximum flow rate and can damage the column if the Purge Valve is closed 5 2 Status Screen 26 The Status Screen shows the most important parameters Mode Intern Status STOP Mode Intern Status RUN Pressure Mode The top line shows the currently
23. orated into the working area side of the instrument In case of a leakage of the Gradient Mixer or Vacuum Degasser solvent will be leaking through this tubing Make sure that the liquids are collected in some vessel and properly disposed of A Element El o o Tm USB RS 444 RS 112 REMOTE 21 4 3 2 Electrical Connections The Drain and Gas Outlets are only connected when the Vacuum Degasser Option is available e Connect the supplied power cord to the Mains connector 5 e Connect the supplied RS 232 cable to the RS 232 connector 2 if the instrument is to be operated by PC e Connect the remote control wires to the Digital I O connector 1 if the instrument is to be run in External mode Please refer to 5 Instrument Operation for further information about PC control and Remote control Element Remote Digital I O RS 232 Serial Communication RS 485 Communication Bus 4 USB Communication not in use Mains Power Supply 22 4 3 3 Remote Connector Run Input Used to start the pump with the current settings EXTERN contact see below must be closed to activate this feature Extern Input The EXTERN input contact must be closed for external operation Inject Input Connection for a manual injection valve The pump transfer the injection signal to the data system via RS 232 Error Output In case of a pump error this output is set REMOTE Pi
24. pressibility as well as the always existing leakage rate of the check valves in relation to the actual pressure The processor is checking the back pressure every 0 1 seconds and corrects the piston speed accordingly The calculation program is using the liquid with the smallest compressibil ity water as a factor of 1 As different organic solvents are showing a different compressibility the processor needs this information for the most accurate delivery rate This information can be added through the Compressibility Factor in the menu As water has been normalized to the factor of 1 all others have to be small er than 1 The following table shows the Compressibility Factors of the mostly used solvents Solvent Compressibility For solvent mixtures of different organic solvents the constant can be cal culated by adding the constants of the different solvents in relation to their percentage in the mixture For example 50 H20 20 Methanol 30 Acetonitril F 0 5 F H2O 0 2 F Methanol 0 3 F Acetonitril F 0 5x 1 0 0 2 x 0 63 0 3 x 0 68 F 0 83 For other solvents with an unknown factor an average factor of 0 700 can be used The difference from the correct delivery rate won t be higher than 5 If this accuracy is good enough a medium factor of 0 700 for all or ganic solvents and 1 000 for water can be used APPENDIX B SPECIFICATIO B 1 Technical Specifications B 1 1 Vacuum Chamber optional
25. tion The back panel of the 9432 HPLC Pump System houses the power and interface connectors and several drain outlets The Power Cord Connection amp Power Switch housing holds the main fuse in an internal fuse carrier The optional Drain Outlet is the leakage drain for the Gradient Mixer Module The optional Gas Outlet is the gas outlet of the Vacuum Pump where all solvent fumes from the degassing process are exhausted The digital 1 O and other interface connectors are used for remote instru ment control and are discussed in a later chapter Element ce Gradient Mixer Module Drain optional Vacuum Degasser Gas Outlet optional Instrument Serial Number Power Cord Connection amp Power Switch Remote Digital I O Connector RS 232 Interface Connector RS 485 Interface Connectors Elo o E amm USA 25 444 RS 212 REMOTE N USB Interface Connector 15 3 2 Functional Description Element gt oump Em a s e 16 3 2 1 Front Elements The 9432 HPLC Pump System has all important parts directly accessible from the front panel when the Front Cover is opened or removed The Capillary Holder 3 holds the pump inlet capillary in place The Solvent Inlets Block 4 has an inlet connection for each of the 2 sol vents A and B Below is the Solvent Outlet Block for connecting the sol vents A nd B to the pump head inlets The Pumpheads 7 9 is completely accessible from
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