Borg Decloaker, RTU
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1. expiration date printed on vial If reagents are stored under conditions other than those specified in the package insert they must be verified by the user taal Biocare Medical 4040 Pike Lane Concord CA 94520 USA Tel 800 799 9499 l CE Page 1 of 2 www biocare net Protocol Recommendations One Step Heat Retrieval 1 Deparaffinize tissues and hydrate to water If necessary block for endogenous peroxidase and wash in DI water 2 Place slides into 1X retrieval solution in a slide container e g Coplin jar Tissue Tek staining dish or metal slide canister 3 Retrieve sections under pressure using Biocare s Decloaking Chamber Follow the recommendations on the antibody data sheet and Decloaking Chamber User Manual 4 Check solution for appropriate color change See Technical Note 1 5 Gently rinse by gradually adding DI water to the solution then remove slides and rinse with DI water Two step Deparaffinization and Heat Retrieval Method with Hot Rinse HTR1001 1 Dry tissue sections for 1 hour at 37 C and then dry slides for 10 30 minutes at 60 C 2 Dilute concentrated Hot Rinse at a ratio of 1 25 1 ml Hot Rinse to 24 ml of deionized water 3 Fill a Coplin Jar Tissue Tek staining dish or metal slide canister with 1X Hot Rinse Fill a second container with 1X Borg Decloaker Place both solutions into the Decloaking Chamber Place the slides into the slide container with the 1X HIER solution2. 7 Retrieve sections under pressure using Biocare s Decloaking Chamber Follow the recommendations on the antibody data sheet and Decloaking Chamber User Manual 8 Check solution for appropriate color change See Technical Note 1 9 Transfer slides to the slide container with 1X Hot Rinse and agitate 20 dips 10 Gently rinse by gradually adding DI water to the solution then remove slides and rinse with DI water Two step Deparaffinization and Heat Retrieval Method with Aqua DePar ADP1002 1 Dry tissue sections for 1 hour at 37 C and then dry slides for 10 30 minutes at 60 C 2 Dilute concentrated Aqua DePar at a ratio of 1 10 1 ml Aqua DePar to 9 ml of deionized water Fill a Coplin Jar Tissue Tek staining dish or metal slide canister with 1X Aqua DePar Fill a second container with 1X Borg Decloaker Place both solutions into the Decloaking Chamber Preheat the solutions for 10 minutes at 65 75 C DC 2002 2008 or 80 C DC NxGen Place the slides in the preheated Aqua DePar and agitate 10 dips Let slides sit in solution for 5 10 minutes 8 Transfer slides into the second container with Borg and agitate 10 20 dips Remove the Aqua DePar solution from the Decloaking Chamber 9 Retrieve sections under pressure using Biocare s Decloaking Chamber Follow the recommendations on the antibody data sheet and Decloaking Chamber User Manual 10 Check solution for appropriate color change See Technical Note 1 11 Gently rins
3. Borg Decloaker RTU Pretreatment Reagent ISO 9001 13485 CERTIFIED Control Number 901 BD1000 021015 Catalog Number BD1000 S 250 MM G1 BDS1000G1 Description 250 1000 ml 1 gal Ready to use Intended Use For In Vitro Diagnostic Use Borg Decloaker RTU is a buffered solution that is intended for laboratory use in heat induced antigen retrieval of formalin fixed paraffin embedded FFPE tissues for immunohistochemistry IHC procedures The clinical interpretation of any staining or its absence should be complimented by morphological studies using proper controls and should be evaluated within the context of the patient s clinical history and other diagnostic tests by a qualified pathologist Summary amp Explanation Borg Decloaker is a heat retrieval solution For many antibodies titers will increase compared to other heat retrieval buffers Borg Decloaker incorporates Assure technology a color coded high temperature pH indicator solution The end user is assured by visual inspection that the solution is at the correct dilution and pH This product is specially formulated for superior pH stability at high temperatures and will help prevent the possibility of losing pH sensitive antigens Borg Decloaker is non toxic non flammable odorless and sodium azide and thimerosal free When used in conjunction with Aqua DePar ADP1002 or Hot Rinse HTR1001 and Biocare s digital electric pressure cooker Decloaking Chamber th
4. IED Control Number 901 BD1000 021015 Limitations The protocols for a specific application can vary These include but are not limited to fixation heat retrieval method incubation times tissue section thickness and detection kit used Due to the superior sensitivity of these unique reagents the recommended incubation times and titers listed are not applicable to other detection systems as results may vary The data sheet recommendations and protocols are based on exclusive use of Biocare products Ultimately it is the responsibility of the investigator to determine optimal conditions The clinical interpretation of any positive or negative staining should be evaluated within the context of clinical presentation morphology and other histopathological criteria by a qualified pathologist The clinical interpretation of any positive or negative staining should be complemented by morphological studies using proper positive and negative internal and external controls as well as other diagnostic tests Quality Control Refer to CLSI Quality Standards for Design and Implementation of Immunohistochemistry Assays Approved Guideline Second edition I LA28 A2 CLSI Wayne PA USA www clsi org 2011 Precautions 1 This product is not classified as hazardous The preservative used in this reagent is Proclin 950 and the concentration is less than 0 25 Overexposure to Proclin 950 can cause skin and eye irritation and irritation to mucous membra
5. e by gradually adding DI water to the solution then remove slides and rinse with DI water Technical Notes 1 RTU or 1X Borg Decloaker is a pale purple color When the solution reaches 80 125 C the solution turns a faint purple color and indicates that the high temperature solution is at correct pH A yellow or red solution indicates an incorrect Nn A Nn Oo N pH 2 If using Biocare s Desert Chamber Pro a programmable turbo action drying oven dry sections at 25 C overnight or at 37 C for 30 60 minutes and then dry slides at 60 C for 30 minutes 3 Use positive charged slides use Biocare s Kling On HIER Slides and cut tissues at 4 5 microns Do not use any adhesives in the water bath Poor fixation and processing of tissues will cause tissue sections to fall off the slides especially fatty tissues such as breast Tissues should be fixed a minimum of 6 12 hours 4 If tissues lose adhesion to the slide during retrieval this can be mitigated by immersion of slides into 10 formalin for 10 minutes after deparaffinization in xylenes and rehydration in alcohols and water 5 Protocol time and temperatures for HIER can vary depending on the Decloaking Chamber model used Please refer to the relevant Decloaking Chamber manual for appropriate protocol times and temperatures EMERGO EUROPE Molenstraat 15 2513 BH The Hague The Netherlands Fax 925 603 8080 Borg Decloaker RTU ISO 9001 13485 Pretreatment Reagent CERTIF
6. e user can deparaffinize and pretreat tissues in only two steps Xylenes and alcohols are not needed for deparaffinization thus saving valuable technician time and reducing costs for reagents and hazardous waste removal Known Applications Immunohistochemistry formalin fixed paraffin embedded tissues Supplied As 250ml Borg Decloaker ready to use BD1000S 250 1000ml Borg Decloaker ready to use BD1000MM Igal Borg Decloaker ready to use BD1000G1 1gal Steam Strips Borg Decloaker ready to use BDS1000G1 Steam Monitor Strips 25 613H Materials and Reagents Needed But Not Provided Microscope slides positively charged Desert Chamber Drying oven Positive and negative tissue controls Xylene Could be substituted with xylene substitute Ethanol or reagent alcohol Decloaking Chamber Pressure cooker Deionized or distilled water Wash buffer TBS PBS Enzyme digestion Avidin Biotin Blocking Kit Labeled Streptavidin Kits Only Peroxidase block Protein block Primary antibody Negative control reagents Detection kits Detection components Chromogens Hematoxylin Bluing reagent Mounting medium Biocare Medical Products Refer to a Biocare Medical catalog for further information regarding catalog numbers and ordering information Certain reagents listed above are based on specific application and detection system used Storage and Stability Store at room temperature Do not use after
7. nes and upper respiratory tract The concentration of Proclin 950 in this product does not meet the OSHA criteria for a hazardous substance Wear disposable gloves when handling reagents 2 Specimens before and after fixation and all materials exposed to them should be handled as if capable of transmitting infection and disposed of with proper precautions Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with reagents and specimens If reagents or specimens come in contact with sensitive areas wash with copious amounts of water 3 Microbial contamination of reagents may result in an increase in nonspecific staining 4 Incubation times or temperatures other than those specified may give erroneous results The user must validate any such change 6 The SDS is available upon request and is located at http biocare net 7 Consult OSHA federal state or local regulations for disposal of any toxic substances Proclin is a trademark of Rohm and Haas Company or of its subsidiaries or affiliates Troubleshooting Follow the antibody specific protocol recommendations according to data sheet provided If atypical results occur contact Buiocare s Technical Support at 1 800 542 2002 tal Biocare Medical EMERGO EUROPE 4040 Pike Lane E Molenstraat 15 Concord CA 94520 2513 BH The Hague USA Page 2 of 2 The Netherlands Tel 800 799 9499 www biocare net Fax 925 603 8080
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