HT300A USER MANUAL
Contents
1. The ESP EZL tips in EZ Load format can be loaded on the head three easy Page 13 of 37 Operating Instructions For The i Pette Liquid Handling System 5 1 INSTALLING THE PIPETTING HEAD Important Note Before you install the pipetting head please make sure e fill volume of machine is not greater than the tip volume of pipetting head e Fornon disposoble tips usage The pipetting head had been cleaned since the last usage and there is no remaining liquid inside tips e For disposable tips usage new set of disposable tips has been loaded onto the pipetting head correctly and there is no remaining liquid inside tips see Section 4 Disposable pipetting Tips Usage for details e There is no foreign object between the plunger block and the pipetting head Move locking lever Slide head into Gently press the Push the locking all the way channels head all the way lever all the way to forward into the pipettor the back 5 2 REMOVING THE PIPETTING HEAD The pipetting head can be easily removed by reversing the above procedure Important Note Due to vacuum suction some gaskets may become stuck to the pipetting head This situation can be easily avoided by carrying out the following step After opening the locking levers wait a few seconds to allow air to get inside The pipetting head will drop automatically once the vacuum diminishes When pipetting head is removed from pipettor user must re define maximum volum2. Range is from 0 to 79 Set to 0 if movement required 14 2 Changing dispense parameters Select Volume found under Dispense title to display input panel Change volume speed and depth values as in section 6 1 14 3 Changing parameters for mixing Select volume under mix title to display input panel Change volume and cycle values as in section 6 1 The aspirating speed and depth for the mix function are set as described in section 6 1 The dispensing speed and depth for the mix function are set as described in section 6 2 Three sets of values for aspirating dispensing and mixing can be selected before running commands Select Value the text will change to Value2 Set the second group of volume speed depth and cycle values Select Value2 the text will change to Value3 Set the third group of values Select Value3 to display the first set of parameters N B A command can be run without changing values each time Example e Pre airgap parameter is set as Valuel aspirate volume as Value2 post airgap as Value3 e Select Aspirate button before executing the second and third aspirate command e Select Valuel without inputing aspirate value each time All three groups of parameters can be saved within a user profile Value 1 Value 2 Value 3 Fig 15 PERFORMING COMMANDS After setting operation parameters select an action from the command panel Fig 7 This allows the user to compl
3. Heath Hall 681 Arrow Grand Circle Helford Road Covina Nether Alderley CA 91722 Cheshire USA SK10 4SY UK Tel 626 966 3299 Fax 626 966 3200 Tel 44 0 1625 861 614 Email info apricotdesigns com Fax 44 0 1625 861 615 Email sales fluidx co uk www fluidx co uk www apricotdesigns com
4. operation Tap to execute mix operation Tap to execute dispense operation Tap to open configuration parameter list Display new file or existing profile name Display and change aspirate depth value Display and change mix volume value Display and change mix cycle value Tap to open auto execution setting window Switch among the three groups of parameter values Tap to open liquid class setup files Tap to switch into another set of execution commands Tap X button to exit 13 USING THE SOFTWARE 13 1 Confirm Machine Model When in Operation Mode the PDA will retrive i Pette details automatically i e model and the original position The i Pette model will be shown within the Configuration Form When in Simulation Mode the default i Pette model is IP 6B 500 13 2 Set Pipetting Head An appropriate pipetting head volume must be set for the i Pette before starting a protocol For i Pette models IP 6B 500 and IP96P 500 default maximum volume is 500ul For i Pette models IP96B 125 and IP96P 125 default maximum volume is 125ul For models IP384B 125 and IP384P 125 default maximum volume is 125ul A message box will appear with option to change maximum pipetting volume Choose yes to enter configuration window max volume change can be made To change to a different pipetting head e Reset the max volume value in the config form Select config to open e Then install the pipetting he
5. the keyboard for input Once saved the note will be displayed when a profile button is selected 17 5 Profile Exist The profile buttons are different colours to show either existing or empty profiles A message stating that the profile cannot be opened will be displayed if the instrument model saved within a profile is not compatible with the current model 18 DEFINE LIQUID CLASS Select Liquid within the command execution panel to display the Define liquid class section Fig 14 This can be divided into several volume ranges with a different calibration value for each range From zero to pipetting head max volume Define liquid class Fig 14 Select the top left side volume button to display volume setting panel Use and buttons to set the volume Fig 15 This volume is the lower limit for the current volume range and will become the upper limit for the next volume range Set up the remaining ranges in the same way E 00 FE a a Next select the top right side calibration value button an input panel will appear Use the and buttons to set the calibration value For model IP96B P 500 Default calibration value 1604 Range 1500 1700 For models IP96B P 125 and IP384B P 125 Default calibration value 6416 Range 6000 6800 Use the same method to set up calibration values for each volume range Select Default button to change setti
6. 3 14 SET OPERATION PARAMETERS 23 14 1 Changing asprirate parameters 24 14 2 Changing dispense parameters 25 14 8 Changing parameters for mixing 25 17 5 Profile Exist 33 19 APPENDIX A 36 1 FEATURES OF THE The i Pette is the ultimate in functionality reliability versatility and value It features e Revolutionary in industry first pipetting head design that enables pipetting with both 384 and 96 well Labware e High volume capacity luL max per channel dispensing volume under 3 CV 125 model e Support full range of ESP disposable tips 15uL 50uL 125uL and custom robotics tips e Control of dispense rate and calibration values allow for pipetting various liquids with accuracy e Manual indexing for serial dilution through simple control lever e Easy to use Pocket PC PPC for calibration and control feedback e Reliable core technology that has been field tested for over two decades and used by major pharmaceutical companies and research laboratories e Easily removable pipetting heads for both disposable tips and fixed tips e Choice of disposable or Teflon coated INSIDE OUTSIDE stainless steel tips e 9 x 14 footprint fits into compatible hoods e Lightweight and portable 2 QUICK REFERENCE DIAGRAMS 1 PPC Controller 2 Pipetting head locking lever 3 Pipetting head 4 Pipetting tips 5 Elevator handle 6 Tray seat 7 ON OFF switch 8 Power co
7. B into any folder under Mobile Device 6 On the PDA go to Start then File Explorer Select the folder which contains the file Setup CAB under My Documents 7 Select Setup and follow installation instructions The updated software will replace the old software within the PDA To run the software Select Start followed by Programs and then the Apricot icon 11 PROGRAM INITIALISATION With the software installed and running the i Pette model will be automatically detected connection made and the appropriate interface entered When the i Pette is turned on and connected initialisation homing tasks for plungers will commence and the software displays Operation Mode Operation Mode allows the user to control all functions and plunger movements of the i Pette A wire connection or wireless connection between the i Pette and PDA is required to use Operation Mode If no connection is available the message Select Correct COM Port or Check Connection will be displayed and the software will enter Simulation Mode NB Simulation Mode is for demonstration purposes only The default i Pette model 96 500 is Used to set parameters and save profiles without the need for PDA connection If there appears to be no connection between i Pette and PDA then first check physical connections Alternatively perform the following actions e Change COM port use COMI for wire connection and COMS for wireless con
8. Software Operating Instructions For The i Pette Liquid Handling System Version 1 Release 3 November 2009 OFluidX Ltd FluidX Ltd Monks Heath Hall Chelford Road Nether Alderley Cheshire SK10 4SY UK T 44 0 1625 861 614 44 0 1625 861 615 E infoGfluidx co uk www fluidx co uk Page 1 of 37 Operating Instructions For The i Pette Liquid Handling System This document is for information only the manufacturer accepts no liability for errors contained herein or for incidental or consequential damages with the furnishing performance or use of this material Unless otherwise specified references to names or parts is purely casual and has the purpose of illustrating the product The contents of this publication may not be reproduced in any form or by any means including electronic storage and retrieval or translation into a foreign language without prior agreement and written consent from the copyright owner The information contained in this document is subject to change without notice All rights reserved TABLE OF CONTENTS 1 FEATURES OF THE 6 2 QUICK REFERENCE DIAGRAMS 7 3 SAFETY PRECAUTIONS 8 3 1 SAFETY INSTRUCTIONS 9 3 2 UNPACKING CRATE 10 MOVING amp INSPECTING THE PIPETTOR 4 OPERATIONS OVERVIEW HARDWARE 12 Setting of of Pipettor 4 2 CONDITIONS OF USE 13 CARE AND MAINTENANCE 16 FOR DISPOSABLE TIPS USAGE 13 1 Confirm Machine Model 23 13 2 Set Pipetting Head 2
9. ad and change tips as required NB The maximum volume setting can be changed without changing pipetting head 14 SET OPERATION PARAMETERS Set parameters for volume speed and cycle within the parameter setting panel Fig 2 before running single execution commands Depth parameters can only be set for instruments fitted with an elevator motor models IP96P 500 IP96P 125 IP384P 125 s z eo 150 Volume Speed Depth Cycle 0 23 of 37 Operating Instructions For The i Pette Liquid Handling System 14 1 Changing asprirate parameters Select Volume found under Aspirate title The following panel Fig 3 will be displayed Cancel Fig p Using the or buttons change each digit as required Select to exit and save new value To change aspirating speed select Speed under Aspirate title the following Fig 4 will be displayed ERE HE Cancel i Fig 4 Using the or buttons change each digit as required Select to exit and save new speed value Range is from 0 to 100 To change depth select Depth under Aspirate title the following Fig 5 will be displayed Fa BEEN 00 Fig 5 Using the or buttons change each digit as required Page 24 of 37 Operating Instructions For The i Pette Liquid Handling System Select to exit and save new depth value
10. aining liquid within the tips will be removed Speed can be changed under dispense title If the instrument does not have an automated stage movement manually adjust the depth for emptying by holding the stage or locking it 15 5 Blow out function Once the emptying process has been completed select Blow out The i Pette will execute Blow out function to remove any remaining liquid attached to the fips If the remaining volume is not greater than lul the blow command can be incorporated into the emptying function 15 6 Auto function Use the auto function to set the automatic execution of a command After setting a command Aspirate Dispense or Mix select auto to open a new window Fig 9 When the stage is raised a sensor is activated set and lock position beforehand the i Pette will perform the pre set command with the values displayed in the current interface This shortcut reduces the need to select Aspirate Dispense or Mix on the panel to start the function The interval of touching sensor is at least 3 seconds when continuing to do any function more than once To disable the Auto function set None in the auto setting window The Auto function can only be used in manual mode by using the touch sensor For automatic mode the sensor block should be locked at the highest position to enable the stage to move freely Aspirate Mix Dispense None F
11. dio Ports Slots Display Display size Input devices Wireless communication Software included ROM Security management Dimensions w x d x h Weight Power Warranty Feature Windows Mobile 5 0 for Pocket PC Premium Edition Pocket versions of Microsoft software are included Outlook Word Excel and Internet Explorer for Pocket Marvell PXA270 processor 520 MHz 64 MB SDRAM 192 MB Flash ROM Flash ROM Integrated microphone Speaker and one 3 5 mm stereo headphone jack MP3 stereo through headphone jack Standard 1 IrDA 1 USB 1 1 Client 1 Serial RS232 1 DC power 1 AC DC connector Rear 1 Charging ActiveSync connector 1 Compact flash 1 secure digital 3 5 in transflective TFT QVGA color touch screen 3 5 inch Transflective TFT QVGA Stylus Touch sensitive display for stylus or fingertip Wi Fi 802 115 Bluetooth wireless technology ActiveSync Adobe Reader for PC ClearVUE Suite Word and Excel ClearVUE Suite PPT and PDF Outlook 2002 Desktop WorldMate NA Retail only Media Player Desktop ProtectTools secured by CREDANT Technologies 3 01 x 0 65 x 4 71 76 6 x 16 3 x 119 4 mm 5 8 oz 164 4 g Removable rechargeable 1440 mAh battery One year parts and labor in most regions 90 days technical support for software in most regions Additional offers may vary by region Manufactured for Manufactured by fluidX Ltd Apricot Designs Inc Monks
12. dioactive substances Immediately remove pipetting head from main unit and clean up stainless steel tips or dispose the disposable tips after operating the instrument When dealing with volatile organic solvents the machine should be operated inside a fume hood 3 1 SAFETY INSTRUCTIONS ALWAYS USE STRAP CUTTERS Use only cutters specifically designed for strap cutting Claw hammers crow bars chisels will cause tensioned strapping to hazardously fly apart CUT STRAPPING CAREFULLY When cutting vertically tensioned strapping stand to one side hold the strapping against the package and cut below the hold The strapping will rebound downward away from you This will help prevent personal injury Other people should remain at a safe distance When cutting horizontally tensioned strapping stand to the left of the cut hold the strapping against the package and cut to the right of the hold The strapping will rebound to the right away from you This will help prevent personal injury Other people should remain at a safe distance Page 9 of 37 Operating Instructions For The i Pette Liquid Handling System 3 2 UNPACKING CRATE Remove six screws from crate 3 on each side Carefully lift and discard the upper part of the crate Cut the three tie wraps that are secured to the base of the crate Machine is ready for relocation Lift by the base while moving Do not lift by the stage platfo
13. e Init i Pipette To Operation Mode Set Operation Parameters Change Config Values Optional Execute Single Command Save Profile 10 TO RUN OR UPDATE I PETTE SOFTWARE The i Pette operating software is normally loaded into the PDA before shipment To turn on press the power button on the right side top of the PDA Go to Start then Programs Use the stylus to select the icon to run the software The software may require updating periodically An updated installation file Setup CAB will be sent to users on CD or via email this file can be transferred from a PC to the PDA The PC must run MS Windows 2000 or MS Windows as the minimum operating system To download the file from a PC Follow step 6 only to download the file into the PDA without using a computer 1 If connecting PDA to the PC for the first time install the file ActiveSync41Setup exe found on the CD supplied with original shipment this sets up communication between the PDA and PC 2 Connect desktop cradle to the PC Attach free end of the USB cable into USB port on the PC If the PDA battery charge level is low then recharge by connecting one end of the AC adapter to the cradle and the other to an electrical outlet 3 Insert PDA into the desktop cradle and turn on 4 Double click My Computer on the PC locate new PDA icon with text Mobile Device 5 Copy updated software installation file Setup CA
14. e of the pipettor during head installing If the pipettor crashed remove pipetting head before restarting the machine and make sure no fluid is left inside the pipetting head Page 14 of 37 Operating Instructions For The i Pette Liquid Handling System 5 3 PLATE HEIGHT turn height positioning screw clockwise The user can perform all the functions by manually raising and lowering the elevator The elevator be locked at any height by turning the elevator handle knob clockwise User can also bring the tray seat to same height by adjusting the height positioning screw A height positioning screw guarantees that the tray seat will stop at set height its locking mechanism will stop the tray seat from traveling beyond set height position To adjust height position slightly loosen the screw by turning counter clockwise bring screw to desired height then lock To lock NOTE Properly setting the plate height will ensure minimal adhesion of liquid to the tips which will increase pipetting accuracy 5 4 EMERGENCY STOP For emergency stop at any time pres the STOP button on the PPC controller Page 15 of 37 Operating Instructions For The i Pette Liquid Handling System 6 CARE AND MAINTENANCE Proper care and maintenance can ensure the efficiency safety and durability of your instrument It is imperative that an appropriate daily cleaning procedure be devised so that the pipetting head assembly does no
15. ete a single command set configuration parameters automatic execution liquid class or profiles cnm uaua sat Fig 7 Select Shift to switch to next group of command buttons Fig 8 mes me praes Fig 8 15 1 Aspirate function Place a filled plate on the stage manually adjust to the required aspirate depth and input value as Depth under Aspirate title To use automatic mode Set Depth value without locking stage if sensor block is unlocked or touches the bottom the stage will not move To use manual mode Manually adjust the aspirate depth and lock the stage Select Aspirate to start aspirating the stage will rise to the depth set in automatic mode or remain at the fixed depth in manual mode The aspirating volume and depth performed will be those seen in the current set of Aspirate parameters If aspirating an airgap allow the stage to move away from the tips Alternatively set Depth value to 0 in automatic mode If the instrument does not allow stage movement the aspirate depth can only be changed manually Hold the stage or lock 15 2 Dispense function Place a plate on the stage manually adjust the stage to set dispense depth and input value as Depth found under Dispense title To use automatic mode Set Depth value without locking stage if sensor block is unlocked or touches the bottom the stage will not move To use manual mode Manuall
16. he i Pette Liquid Handling System 17 CREATE USER PROFILES User Profiles enable users to save set operation parameters the auto execution settings and configuration settings There are nine profiles available When entering the main interface the last set of operation and configuration parameters selected are shown To save current settings as profile or to open a previously saved profile select the Profile within the command execution panel the Select Profile form will be displayed Fig 13 Profile 1 Profile 2 Profile 3 Profile 4 Profile 5 Profile 6 Profile 7 Profile 8 Profile 9 Select a profile First then click Save or Open Fig 13 17 1 Save Profile Select a Profile button and tap Save The current settings for operation auto execution and configuration parameters are saved in the user profile The profile window will close and the name will be shown within the title of the main interface 17 2 Open Existing Profile Select Profile button and tap Open The selected profile will open All parameter values for operation auto execution and configuration settings are retrieved from the profile and the profile name will be shown in the title of the main interface 17 3 Exit Select Exit to leave this section without making changes 17 4 Notes The Notes text box allows the user to input a simple note e g name of profile creator purpose etc To enter note click on small keyboard icon to open
17. ig 9 15 7 Initialisation If initialising is required e g in the case of communication difficulties select the Init button to commence 15 8 Default Set default values for all operation parameter settings three groups configuration parameter settings and auto execution setting 15 9 Stopping Pushing the red emergency stop button will terminate the operation immediately Please note that in this instance the remaining volume in the tips may not be calculated accurately If possible empty tips before performing another function After stopping one function the i Pette is able to perform additional commands directly without the need for initialisation or other action beforehand 15 10 Changing pipetting heads Pipetting heads can be changed on the following i Pette models IP 6P 500 IP96P 125 IP384P 125 Select Liquid display will change to Chg Head The select Chg Head to unlock the pipetting head After fitting the new head select OK to lock 16 SET CONFIGURATION PARAMETERS Select the Config within the command execution panel to display the Config Form Fig 10 Select and change the value buttons on the right side as needed Select the name on the left side restore the default values if necessary Self Test will activate i Pette life test The i Pette will continuously perform aspirating and dispensing functions according to the set pa
18. is at the OFF position Connect the power entry connector and a power outlet with ground connection using the power cord provided Your pipettor is now ready for testing The i Pette is a high precision instrument designed for high throughput liquid operations using 96 or 384 well microplates These operations most commonly begin with the installation of the pipettor head into the pipettor main unit The user has the choice of two types of pipettor heads type 1 Pipettor head with non disposable stainless steel tips type 2 Pipettor head with disposable tips made of polypropylene For this option the disposable tips are installed onto the pipettor head prior to operation according to the following instructions Once the pipettor head is installed the user generally places a source labware containing specific liquid onto the elevator The user then fills the tips using the FILL or FILL VOLUME commands The source labware is then replaced with the destination labware which is then dispensed upon with the contents of the tips using the DISPENSE or EMPTY commands Upon completion of the operations for non disposable tips Clean non disposable tips using the head washing station for disposable tips Eject tips using the tip ejector and discards the tips A manual indexing option is available for the 96 channel I PIPETTE to enable it to work with 384 well labware Refer to the i Pette PPC GUI operating manual for details on how to perfo
19. n the unit regularly with a clean cloth moistened with a mild solvent or cleanser Avoid cleaning the LCD area with solvent or cleanser These can dull the surface and damage the LCD Maintain the cleanliness of your work area 7 TROUBLESHOOTING Problem Leakage Signs Uneven liquid levels among the tips drippings from tip s even when EMPTY or DISPENSE commands are not in use Solution Apply thin film of mineral oil to the gasket s suspected of leaking Make sure there is no foreign object s between the plunger block and the pipetting head Make sure both the locking levers are at horizontal positions Check the top of the pipetting head make sure there is no missing or damaged gasket s If disposable tips are used make sure tips are loaded correctly Every tip has to cover both o rings Problem Machine crashed Solution Remove pipetting head before restarting the machine and make sure no fluid left inside the pipetting head 8 SPECIFICATIONS e Dispensing Precision 500 376 CV at 5 ul 125 lt 3 CV at 1 ul e Dispensing Accuracy 500 1 at 10 ul 125 176 at 1 ul e Resolution 500 1 ul 125 0 1 ul e Pipette Volume 500 5 500 ul 125 1 125 ul e Dimensions 9 W x 14 D x 19 H 229W x 356D x 483H mm e Weight Approx 44 Ibs 20 kg e Power 100 to 240 VAC 50 60 HZ e Operating Temperature 50 100 F 9 OPERATIONS OVERVIEW SOFTWARE Turn On i Pipette Start AIP GUI To Simulation Mod
20. nection in Config file then tap Init to perform initialization without exiting the interface e Orexit and open the program again e Orturn off and then turn on the i Pette power If it is not possible to exit the program locate the Reset button on the left side bottom of the PDA insert the stylus into the small hole and press Reset Wait for the PDA to initialise as normal then open program again 12 MAIN SCREEN OVERVIEW gogg gogg zoog zoog AIP Profile x BA 2271176 Fig 1 21 37 Operating Instructions For The i Pette Liquid Handling System Key for Fig 1 CON An RI WYN a oS 4 ao MA WN O 17 18 Tip Volume Bar Aspirate Volume Input Button Aspirate Speed Input Button Dispense Volume Input Button Dispense Speed Input Button Dispense Depth Input Button Aspirate Execution Button Mix Execution Button Dispense Execution Button Configuration File Button Title Bar Aspirate Depth Input Button Mix Volume Input Button Mix Cycle Input Button Auto Execution Setting Button Value Group Switch Button Liquid Class Setup Button Execution Command Switch Button Exit Button Display current actual remaining volume in the tips Display and change aspirate volume value Display and change aspirate speed value Display and change dispense volume value Display and change dispense speed value Display and change dispense depth value Tap to execute aspirate
21. ngs back to default values Fig 16 To save the current class setting into a class file or open an existing class file to operate select File within the default liquid class window The Liquid Class File form will appear Fig 17 Up to 9 files can be saved Page 34 of 37 Operating Instructions For The i Pette Liquid Handling System z S 5 Class 1 Class 7 Select a liquid class then click Save Open Fig 17 18 1 Save Class File Select a Class button followed by Save The current class setting is saved 18 2 Open Existing Profile Select a Class button then Open An option to save the current file will appear followed by the class file selected Class setup is retrieved from the class file and is seen within the Define liquid class window when opened 18 3 Exit Select Exit to exit without making changes 18 4 Notes The Notes text box allows the user to input a simple note such as a description for the class file The note is displayed when the associated Class button is selected 18 5 Class File Exist Buttons for the existing class files and the empty files will appear in different colours to differentiate A message will appear stating that the class file cannot be opened if the instrument model saved within a profile is not compatible with the model in use 19 APPENDIX A SPECIFICATIONS Operating system Processors available Memory Flash memory Au
22. nnector 9 Serial connector 10 Elevator height lock Page 7 of 37 Operating Instructions For The i Pette Liquid Handling System 3 SAFETY PRECAUTIONS The i Pette is designed and engineered to meet or exceed all safety standards Nevertheless careless or abusive operation of these machines may still lead to possible hazardous situations Please comply with the following guidelines Never open the instrument there are user serviceable parts inside In the rare that you experience problems with your machine please follow the remedies under Section 7 Troubleshooting in this manual If this does not solve the problem contact FLUIDX LTD Only operate the instrument in an indoor environment Avoid direct exposure to sunlight and excessive humidity Avoid operating the machine with wet hands Always connect the machine to a power outlet with ground connection Use only optional equipment and peripherals supplied by FLUIDX LTD Using any other add on products or modifications may affect the performance of your machine and jeopardize the operator s safety In no circumstances should any part of the operator s body or any foreign object s except the labware in use enter the area directly under the pipetting tips and above the elevator In case of emergency the machine can be stopped at any time by pressing the STOP button Always wear appropriate protective clothing goggles when dealing with corrosive or ra
23. rameters until stopped by the user Pipettor Model IP96B 500 COM Port COM1 Max Volume uL Over Shoot uL inis ico Fig 10 16 1 i Pipette Model Number The i Pette model is shown within this panel Fig 10 but cannot be changed using this form 162 COM Ports The default communication port is COMI for a wire connection between PDA and i Pette If using a wireless connection remove the wire cable from the PDA and use wireless connector activate the wireless function in PDA After entering the software go to Config window select COM Port button select Select the Init button to initialise 16 3 Max Volume Select the value button for Max Volume to display the Input Panel Set the max volume by using the and buttons 16 4 Over Shoot For model IP96B P 500 Default over shoot value 1201 value 48 For models IP 6B P 125 and IP384B P 125 Default over shoot value 3uL Max value 12UL Select the Over Shoot value button to display the input panel Fig 11 Use buttons to set EEN Fig 11 16 5 Plunger Pause The plunger waiting interval can be adjusted as required Default Medium Select the Plunger Pause value button a new window will open Fig 12 to allow pause length to be chosen Q Very Short Medium Q Very Long Fig 12 Page 31 of 37 Operating Instructions For T
24. rm Page 10 of 37 Operating Instructions For The i Pette Liquid Handling System 3 3 MOVING amp INSPECTING THE PIPETTOR Lift the unit up by placing your hands at the spots indicated by the two arrows Keep your back straight to avoid possible injury we recommend this task to be handled by two people together as the unit is quite heavy Place the unit on a steady sturdy flat and level surface Carefully remove items from the accessories boxes Avoid piercing the box with a long sharp object as this may damage the contents After unpacking the pipettor inspect the unit for visible damage that may have occurred during transportation such as scratches or dents in the casing bent tips etc If you notice any damage to the unit contact both the freight carrier and FLUIDX LTD immediately DO NOT PLUG IN OR TURN ON THE SYSTEM Page 11 of 37 Operating Instructions For The i Pette Liquid Handling System 4 1 OPERATIONS OVERVIEW HARDWARE Setting of of Pipettor After confirming that there is no damaged or missing item begin to test the pipettor Please note that this testing procedure should be performed only after you have read through the entire instruction manual Position the pipettor on top of a sturdy safe clean surface For detailed information on how to choose a location please refer to the sections under 3 1 Safety precautions and 3 9 Conditions of use Make sure the ON OFF switch
25. rm the pipettor operations 4 2 CONDITIONS OF USE The i Pette is designed to perform optimally and safely under normal laboratory settings Use of these instruments in other environments is not recommended FLUIDX LTD will not be responsible for any unsatisfactory results if the instrument is no operated under the conditions and intent outlined in this instruction manual The following is a list indicating some of the ideal operational parameters for you instrument This is not an exclusive list please contact FLUIDX LTD if you have further questions Environment indoor temperature regulated non dusty Operation Temperature 50F 100F Voltage 100V 240 Size of particles in liquid less than 0 032 diameter to prevent blockage of tips This unit should not be used in a moving environment or relocated frequently DISPOSABLE TIPS AND HEAD USAGE The EZ Load pipettor heads from FLUIDX simplify the use of disposable tips for multiple channel pipetting by making tip loading and unloading a simple manual process without the requirement for any tools The EZ Load heads are used in conjunction with specially made disposable pipette tip racks that allow the transfer of a rack of tips directly from the package into the EZ Load head The tops of the pipette tips are sealed against the face seal by the pipettor s automatic electric loading mechanism A reliable seal is provided for optimal pipetting performance
26. t become contaminated or attacked by harsh solvents Please follow these guidelines carefully Grade mineral oil can be purchase at drug store We do not supply mineral oil 6 1 6 2 FOR DISPOSABLE TIPS USAGE Always eject tips from pipetting head after use Never leave tips on pipetting head overnight Lubricate pipetting head o rings every other pipetting with the supplied lubrication box Refill box with heavy grade mineral oil when necessary Never expose pipetting head o rings to UV light as this will cause damage to the rings GENERAL MAINTENANCE Periodically apply a thin layer of mineral oil to the bottom of the plunger block see picture in section 3 4 in order to promote proper vacuum seal Caution use mineral oil ONLY over lubrication may produce adverse effect Regularly inspect the underside of the plunger block as well as the top of the pipetting head for dirt or foreign objects Failure to do so may severely jeopardize the performance of the instrument Inspect all moving parts regularly Carefully remove any dirt or foreign objects Lubricate with high quality instrument grade lubricant that contains silicone or molybdenum when necessary Visually inspect the stainless steel tips or disposable tips every time before a pipetting session Replace any bent stainless steel tips or damaged disposable tips before proceeding Regularly inspect the instrument for worn components and replace them immediately Clea
27. y adjust the dispense depth and lock the stage Select Dispense to start dispensing Volume and speed will be those shown in current parameter settings If the instrument does not have an automated stage movement the dispense depth can be changed manually Adjust the dispense depth hold the stage or lock the stage to dispense 15 3 Mixing function Place a plate on the stage Manually adjust the stage to set the aspirate or dispense depth of the mix Input values in Depth found under Aspirate and Dispense titles To use automatic mode Set the aspirate and dispense values without locking stage if sensor block is unlocked or the stage touches the bottom the stage will not move To use manual mode Manually adjust the mix depth and lock the stage Select Mix to start Volume and speed for mix cycles will be those seen in the current parameter settings If the instrument is not fitted with an automated stage movement mix depth can be adjusted manually Hold the stage or lock stage to dispense 15 4 Emptying function Place a plate on the stage manually adjust the stage to the required depth to empty Input depth value under dispense title To use automatic mode Set the Depth value without locking stage if sensor block is unlocked or touches the bottom the stage will not move To use manual mode Manually adjust the emptying depth and lock the stage Select empty and any rem
Download Pdf Manuals
Related Search