RaPID Assay PCB Test Kit
Contents
1. for 0 25 mL to 1 25 mL dispensing volumes 5 e Pipet tips for adjustable volume pipet 100 1000 uL NOTE Order replacement Combitips and pipet tips separately See the Ordering Information section Materials Required but Not Provided e Methanol HPLC grade or equivalent for water analysis e Protective clothing e g latex gloves e Absorbent paper for blotting test tubes e Liquid and solid waste containers e Marking pen e Instructional video optional Suggestions for Pipettor Use e Practice using both pipettes adjustable volume and Repeater pipettor with water and extra tips before you analyze your samples e Use a new tip each time you use the Repeater pipettor to pipette a different reagent to avoid reagent cross contamination Tips can be rinsed thoroughly dried completely and reused By using the same tip to dispense the same reagent each time you can avoid cross contamination NOTE Repeator tips should be changed periodically after 10 uses since precision deteriorates with use RaPID Assay PCB Test Kit e Draw the desired reagent volume into the Repeater pipettor and dispense one portion of the reagent back into the container to properly engage the ratchet mechanism If you do not do this the first volume delivered may be inaccurate e To add reagents using the Repeater pipettor pipette down the side of the test tube just below the rim e When adding samples and standard using th2. tube 1 to 2 seconds at low speed to minimize foaming Pipetting of magnetic particles should be kept to 2 minutes or less 5 Incubate 15 minutes at room temperature 6 After the incubation combine the upper rack with the magnetic base and press all tubes into the base allow 2 minutes for the particles to separate 7 With the upper rack and magnetic base combined use a smooth motion to invert the combined rack assembly over a sink and pour out the tube contents NOTE If the rack assembly inadvertently comes apart when lifting to pour out tube contents re combine and wait an additional 2 minutes to allow particles to separate 8 Keep the rack inverted and gently blot the test tube rims on several layers of paper towels It is important to remove as much liquid as possible but do not bang the rack or you may dislodge the magnetic particles and affect the results 9 Set the Repeater Pipettor dial to 4 and put on the tip labeled Wash Add 1 mL of Washing Solution down the inside wall of each tube by using the technique described earlier Vortex tubes for 1 2 seconds Wait 2 minutes and pour out the tube contents as described previously Repeat this step one more time NOTE The number of washes and wash volume are important in ensuring accurate results 10 Remove the upper rack with its tubes from the magnetic base With the Color tip attached to the Repeater Pipet and the dial set to 2 add 500
3. uL of Color Reagent down the inside wall of each tube as described previously Vortex 1 to 2 seconds at low speed 11 Incubate 20 minutes at room temperature During this period add approximately 1 mL of Washing solution to a clean tube for use as an instrument blank for Results Interpretation 12 After the incubation position the Repeater pipettor at Setting 2 and use the Stop tip to add 500 uL of Stop solution to all test tubes 13 Proceed with results interpretation WARNING Stop solution contains 2M sulfuric acid Handle carefully Results Interpretation 1 After addition of Stop Solution to the test tubes results should be read within 15 minutes 2 Wipe the outside of all antibody coated tubes prior to photometric analysis to remove fingerprints and smudges Photometric Interpretation Using the RPA I 1 The RPA I photometer provided in the Rapid Assay Accessory kit can be used to calculate and store calibration curves It is preprogrammed with vatious RaPID Assay protocols For the PCB RaPID Assay test kit parameter settings are as follows Data Reduct Lin Regression RaPID Assay PCB Test Kit Xformation Ln LogitB Read Mode Absorbance Wavelength 450 nm Units PPB Ret Blk 0 Calibrators of Cals 4 of Reps 2 Concentrations 1 0 00 ppb 2 0 25 ppb 3 1 00 ppb 4 5 00 ppb Range 0 10 5 00 Correlation 0 990 Rep CV 10 NOTE Prior to analys
4. 7EB Procedural Notes and Precautions e Prepare soil samples for analysis according to the procedure in the SDI Sample Extraction Kit Users Guide e After extraction and dilution of samples follow the immunoassay procedure as described in the Rapid Assay PCB Test Kit User s Guide e The initial 2x dilution described for water samples in Step 1 of Collect Store the Sample does not need to be performed for soil samples Quality Control A control solution at approximately 3 ppb as Aroclor 1254 is provided with the PCB RaPID Assay Kit It is recommended that it be included in every run and treated in the same manner as unknown samples If running standard soil procedures an acceptable result should be 2000 times the value stated on the control vial i e 6 0 or 1 2 ppm when the control results are corrected for the dilution factors see Results section below Results Interpretation Interpret soil sample results as described in the RaPID Assay PCB Test Kit procedure accounting for the total dilution factor indicated in the table of the SDI Sample Extraction Kit Users Guide Alternatively program the RPA 1 Analyzer as listed below to automatically correct for this dilution factor 1 The RPA I photometer provided in the Rapid Assay Accessory kit can be used to calculate and store calibration curves To obtain soil results from the PCB Rapid Assay test kit on the RPA I the following parameter settin
5. STRATEGIC DIAGNOSTICS INC RaPID Assay PCB Test Kit A00133 A00134 Intended Use The RaPID Assay PCB Test Kit can be used as a quantitative semi quantitative or qualitative enzyme immunoassay EIA for the analysis of PCB polychlorinated biphenyl in water groundwater surface water well water For applications in other matrices please contact our Technical Service department or refer to the soil application procedure provided The RaPID Assay PCB Test Kit allows reliable and rapid screening for PCB measured and reported as Aroclor 1254 with quantitation between 0 5 and 10 ppb as Aroclor 1254 in water The minimum detection level of the kit is 0 2 ppb as Aroclor 1254 Test Principles The PCB RaPID Assay kit applies the principles of enzyme linked immunosorbent assay ELISA to the determination of PCB and related compounds The sample to be tested is added along with an enzyme conjugate to a disposable test tube followed by paramagnetic particles with antibodies specific to PCB attached Both PCB which may be in the sample and the enzyme labeled PCB the enzyme conjugate compete for antibody binding sites on the magnetic particles At the end of an incubation period a magnetic field is applied to hold the paramagnetic particles with PCB and labeled PCB analog bound to the antibodies on the particles in proportion to their original concentration in the tube and allow the unbound reagents to be decanted Af
6. detection limit MDL which is the lowest concentration of the compound in water that can be picked up in the assay The limit of quantitation LOQ is an approximate concentration in water required to yield a positive result at the lowest standard This is the lowest concentration of the compound that can be quantified in the assay The IC50 is the concentration required to inhibit one half of the color produced by the negative control It is also used to calculate cross reactivity values to similar compounds Compound MDL LOQ IC50 ppb ppb ppb Aroclor 1254 0 20 0 50 3 6 Aroclor 1260 0 20 0 32 2 3 Aroclor 1248 0 22 0 59 4 22 Aroclor 1242 0 34 1 22 8 8 Aroclor 1262 0 36 0 66 4 74 Aroclor 1232 0 84 2 61 18 76 RaPID Assay PCB Test Kit Aroclor 1268 0 92 3 03 21 80 Aroclor 1016 0 94 3 56 25 60 Aroclor 1221 13 54 22 58 162 60 The following compounds demonstrated no reactivity in the PCB RaPID Assay test kit at concentrations up to 10 000 ppb Biphenyl 2 5 Dichlorophenol 2 3 5 Trichlorophenol Di n octyl phthalate The presence of the following substances up to 250 ppm were found to have no significant effect on PCB RaPID Assay results copper nickel zinc mercury manganese phosphate sulfate sulfite magnesium calcium nitrate and thiosulfate Humic acid up to 25 ppm and iron to 100 ppm were found to have no significant effect In addit
7. e Adjustable Volume Pipet To Set or Adjust Volume Press release button on side of pipette and turn the push button to adjust volume up or down Volume setting is displayed on top of pipet See kit instructions for appropriate setting Pipet will accurately dispense volumes between 100 and 1000 uL To Assemble Pipet Tip Gently push nose cone of pipet firmly into a pipet tip contained in the pipet tip rack To Withdraw Sample Keep pipet almost vertical With tip mounted in position on pipet press push button to 1t stop and hold it Place tip at bottom of liquid sample and slowly release push button to withdraw measured sample Ensure that no ait bubbles exist in the pipette tip If bubbles exist dispense sample and re withdraw Slide tip out along the inside of the vessel To Dispense Sample Wipe any liquid from outside of tip taking care not to touch orifice Place tip into tube almost to the bottom and slowly press push button to 24 stop Hold push button at 2 4 stop when removing tip from tube To Eject Tip Press push button to 3 4 stop Tip is ejected STRATEGIC DIAGNOSTICS INC RaPID Assay PCB In Soil Application Intended Use Por detection of Polychlorinated Biphenyls PCB s as Aroclor 1254 in soil For testing in other matrices please contact our technical support department at 1 800 544 8881 Materials Required but Not Provided SDI Sample Extraction Kit Part Number A00137EA A0013
8. e positive displacement pipettor always pipette into the bottom of the tube without touching the sides or bottom of the tube e Use a new adjustable volume pipet tip each time you pipette a new unknown Assay Procedure Prior to performing your first Rapid Assay please take time to read the package inserts in their entirety and review the videotape if available On site training is strongly recommended for new users of this test system Please contact your account manager for further information This procedure is designed for quantitative analysis For running the kit semi quantitatively or qualitatively please contact Technical Support Collect Store the Sample The following steps explain how to properly collect and store your samples 1 Water samples should be collected in glass vessels with teflon cap liners Immediately upon collection water samples should be diluted with an equal volume 1 1 of methanol HPLC grade to prevent adsorptive losses to the glass containers This is a 2x dilution which must be accounted for when interpreting results See Results Interpretation Section 3a for further details Use this diluted sample as sample in Perform the Test NOTE This 2x dilution is not required for soil samples 2 Samples should be collected in appropriately sized and labeled containers 3 If testing soil samples follow the SDI Sample Extraction Kit User s Guide or the appropriate techn
9. ed Results e CV coefficient of variation between standard duplicates of 10 or less e Absorbance reading for the 0 ppb standard should be between 0 8 and 2 0 for all assays e Correlation r of 0 990 or greater for all assays e Kit control within range specified on vial e Absorbance of negative control and standards should be as follows Negative Control gt Std 1 gt Std 2 gt Std 3 RaPID Assay PCB Test Kit 3 Concentrations will be indicated for all samples on the RPA I printout a The concentration as indicated on the printout is multiplied by the appropriate dilution factor if applicable introduced in the procedure The quantitation range of the kit is also multiplied by this factor EXAMPLE Water samples were diluted 2 fold with methanol upon collection see Collect Store the Sample in this User s Guide As a result the concentrations listed on the printout should be multiplied by 2 to determine the sample concentration The standard concentrations are also multiplied by 2 to give a quantitation range in water for this test kit of 0 5 to 10 ppb b Samples with an nd and no concentration listed have an absorbance greater than the negative control therefore no concentration can be computed for these samples Results must be reported as lt 0 5 ppb or Standard 1 multiplied by the dilution factor c Samples with an nd next to a listed concentration have an estimated concentra
10. fety To receive an MSDS for this product visit our web site at www sdix com Copyright 1997 Strategic Diagnostics Inc Z00245 1 Rev 4 4 00 Operation of the Repeater Pipet To Set or Adjust Volume To determine the pipetting volume the dial setting 1 5 is multiplied by the minimum pipetting volume of the tip indicated on the side of the Combitip e g 1 100 uL To Assemble Pipet Tip Slide filling lever down until it stops Then raise the locking clamp and insert the tip until it clicks into position Be sure the tip plunger is fully inserted into the barrel before lowering the locking clamp to affix the tip in place To Fill Tip With tip mounted in position on pipet immerse end of tip into solution Slide filling lever upward slowly Combitip will fill with liquid To Dispense Sample Check the volume selection dial to ensure pipetting volume Place tip inside test tube so that tip touches the inner wall of tube Completely depress the pipetting lever to deliver sample NOTE Dispense one portion of reagent back into the container to engage the ratchet mechanism and ensure accuracy To Eject Tip Empty tip of any remaining solution into appropriate container by pushing filling lever down Raise locking clamp upward and remove the Combitip Adjustable Volume Pipet Push button 1 Stop 24 Stop 3rd Stop Release button Pipet tip Nose cone Pn Repeater Pipet Operation of th
11. gs are recommended Data Reduct Lin Regression Xformation Ln LogitB Read Mode Absorbance Wavelength 450 nm Units f PPM Rgt Blk 0 Calibrators of Cals 4 of Reps 2 Concentrations 1 0 00 PPM 2 f 0 50 PPM 3 f 2 00 PPM 4 10 00 PPM Range 0 5 10 00 Correlation 0 990 Rep CV 10 Performance Data The PCB RaPID Assay does not differentiate between PCB and other related compounds The table below shows compounds at the method detection limit MDL which is the lowest concentration of the compound in soil that can be picked up in the assay The limit of quantitation LOQ is an approximate concentration required to yield a positive result at the lowest standard this is the lowest concentration of the compound in soil that can be quantified in the assay The IC50 is the concentration in soil required to inhibit one half of the color produced by the negative control It is also used to calculate cross reactivity values to similar compounds Compound MDL LOQ IC50 ppm ppm ppm Aroclor 1254 0 20 0 5 3 60 Aroclor 1260 0 20 0 3 2 30 Aroclor 1248 0 22 0 6 4 22 Aroclor 1242 0 34 1 2 8 80 Aroclor 1262 0 36 0 7 4 74 Aroclor 1232 0 84 2 6 18 76 Aroclor 1268 0 92 3 0 21 80 Aroclor 1016 0 94 3 6 25 60 Aroclor 1221 13 54 22 6 162 6 Soil Contaminants Some contaminants found in soils that also contain PCB s can interfere with the analysis and cau
12. han standard kit level is desired contact SDI for special instructions Water samples should be diluted 1 1 in methanol as described in Collect Store the Sample Please follow the instructions from the SDI Sample Extraction Kit to prepare and dilute the soil extract prior to running the assay RaPID Assay PCB Test Kit Perform the Test 1 Separate the upper rack from the magnetic base Place labeled test tubes into the rack 2 Add 200 uL of standards control or samples to the appropriate tubes using the adjustable volume pipet with the dial set on 0200 The negative control standards and control must be run with each batch of samples NOTE Sample should be added to the bottom of the tube by inserting the pipet tip into the tube without touching the sides or the bottom of the tube Take care not to contact sample with pipette tip once dispensed into bottom of the tube 3 Using the Repeater Pipettor with the Conjugate tip attached and the dial set on 1 add 250 uL of Enzyme conjugate down the inside wall of each tube Aim the pipet tip 14 to 2 below the tube rim or tube wall deliver liquid gently to avoid splashback 4 Thoroughly mix the magnetic particles by swirling avoid vigorous shaking and attach the Particles tip to the Repeater Pipettor With the dial set on 2 add 500 uL of magnetic particles to each tube aiming down the side of the tube as described above Vortex mixing each
13. ical bulletin to properly collect and store your sample 4 Samples should be tested as soon as possible after collection If this is not possible storage at 4 C 39 F is recommended to minimize evaporative losses Set Up 1 Remove kits from refrigerator All reagents must be allowed to come to room temperature prior to analysis Remove reagents from packaging and place at room temperature at least 1 hour prior to testing 2 Turn on the RPA 1 or other spectrophotometer The RPA 1 should be warmed up for at least 30 minutes prior to the run 3 Label five 12 5 mL Combitips Conjugate Particles Wash Color and Stop In addition add the name of the compound you are testing for to each Combitip 4 Remove nine clean blank test tubes for standards and control and one test tube for each sample if testing in singlicate Label the test tubes according to contents as follows Tube Contents 1 Negative control replicate 1 Negative control replicate 2 Standard 1 replicate 1 Standard 1 replicate 2 Standard 2 replicate 1 Standard 2 replicate 2 Standard 3 replicate 1 Standard 3 replicate 2 Control 0 Sample 1 1 Etc ere OANA MNF WNW Label at top of tubes to avoid interference with reading of tubes in photometer Sample Extraction Filtration and Dilution Filtration may be necessary to remove gross particulate from the water sample If testing samples at levels higher t
14. ion sodium chloride concentrations up to 1 0 M showed no effect on results Precautions e Training is strongly recommended prior to using the RaPID Assay test system Contact Strategic Diagnostics for additional information e Treat PCB solutions that contain PCB and potentially contaminated samples as hazardous materials e Use gloves proper protective clothing and methods to contain and handle hazardous material where appropriate e Reagents must be added in a consistent manner to the entire rack A consistent technique is the key to optimal performance Be sure to treat each tube in an identical manner e Water samples should be at a neutral pH prior to analysis Samples containing gross particulate should be filtered e g 0 2 um Anotop 25 Plus Whatman Inc to remove particles e Store all test kit components at 2 C to 8 C 36 F to 46 F Storage at ambient temperature 18 C to 27 C ot 64 F to 81 F on the day of use is acceptable Test tubes require no special storage and may be stored separately to conserve refrigerator space e Allow all reagents to reach ambient temperature 18 C to 27 C or 64 F to 81 F before beginning the test This typically requires at least 1 hour to warm from recommended storage conditions Do not freeze test kit components or expose them to temperatures above 100 F 39 C Do not use test kit components after the expiration date Do not use reagen
15. is the RPA I User s Manual should be thoroughly reviewed for more detailed operation instructions EVALUATING TUBE REMOVE TUBE Beep Follow prompts to read tubes Remove tube NOTE Tube order is important The RPA I expects to see the standards in ascending order in duplicate starting with the negative control PRINTING DATA PRINTING CURVE CTRL 1 REP 1 INSERT TUBE EVALUATING TUBE REMOVE TUBE Beep Following evaluation of all standards the instrument will display Data will print Curve will print only if programmed to print See RPA1 Uset s Manual Insert Control Tube Remove Tube of all tubes Instrument Display SELECT COMMAND RUN PROTOCOL SPL REPLICATES 1 5 BLANK TUBE INSERT TUBE EVALUATING TUBE REMOVE TUBE Beep CAL 1 REP 1 INSERT TUBE 2 Follow the instrument prompts to read the absorbance Operator Response Press RUN Scroll using the YES or NO keys until the desired protocol appeats Then press ENTER Press 1 for analysis of samples in singlicate Press ENTER Insert blank tube containing 1mL wash solution Remove tube Insert Tube 1 EDIT CALIBRATORS Press NO if editing is YES NO necessary press YES and refer to the RPA1 User s Manual SPL 1 REP 1 Insert first sample tube INSERT TUBE EVALUATING TUBE REMOVE TUBE Beep Remove tube Continue to follow prompts After all samples have been read press STOP Expect
16. ple concentrations as outlined below 1 Calculate the mean absorbance for each of the three standards and the negative control 2 Determine the standard deviation and CV coefficient of variation of each standard and ensure CV is less than 10 for each 3 Calculate the B Bo for each standard by dividing the mean absorbance value for the standard by the mean absorbance value for the negative control and multiplying the results by 100 4 Construct a standard curve by plotting the B Bo for each standard on the vertical logit y axis versus the corresponding analyte concentration on the horizontal logarithmic x axis on the graph paper provided in the test kit Graph papers are specific for each method Use only the graph paper supplied with each kit 5 Draw the best straight line through all points Using the B Bo of the sample the concentration can be interpolated from the standard curve 6 Multiply results by the appropriate dilution factor if applicable introduced in the procedure For example if the sample was diluted 10 fold to increase the detection levels of the kit then the results must be multiplied by 10 This dilution also changes the range of the assay standards by the same factor NOTE Do not forget to account for the 2x dilution introduced in the Collect Store the Sample procedure for water samples RaPID Assay PCB Test Kit Limitations of the Procedure The Rapid Assay PCB Test Ki
17. reservative and stabilizers A 4 mL volume is supplied in one vial e Diluent Zero Standard Buffered saline containing preservative and stabilizers without any detectable PCB 30 test kit one 10 mL vial 100 test kit one 35 mL vial e Color Solution containing hydrogen peroxide and 3 3 5 5 tetramethylbenzidine in an organic base 30 test kit one 20 mL vial 100 test kit one 65 mL vial e Stop Solution containing a solution of 2M sulfuric acid 30 test kit one 20 mL vial 100 test kit one 60 mL vial e Washing Solution containing preserved deionized water 30 test kit one 70 mL vial 100 test kit one 250 mL vial e Polystyrene test tubes 30 test kit one 36 tube box 100 test kit three 36 tube boxes e Users Guide Materials Required and Ordered Separately See Ordering Information for the appropriate catalogue numbers Rapid Assay Accessory Kit Accessory equipment may be rented or purchased from Strategic Diagnostics See Ordering Information for the appropriate catalogue numbers The accessory kit contains the following items e Adjustable Volume Pipet e Eppendorf Repeater Pipettor e Electronic timer e Portable balance capable of weighing 10 g for soil samples e Vortex mixer e Magnetic separation rack e RPA I RaPID Analyzer or equivalent spectrophotometer capable of reading 450 nm in a 1 mL sample size Other Items e 12 5 mL Combitips for the Repeater pipettor
18. se false positives false negatives or both when the compound is present at elevated concentrations Interferences were assessed by adding increasing concentrations of some relevant contaminants to blank and PCB spiked soils prior to the extraction procedure The concentration of the compound shown below produced no evidence of interference in a positive or negative direction in the 500 ppb to 10 ppm detection range of the procedure described above concentration in soil soil contaminant producing no interference trichloroethylene 100 000 ppm or 10 gasoline 25 000 ppm or 2 5 transformer oil 5 000 ppm or 0 5 1 chloronapthalene 2 000 ppm or 0 2 1 2 4 trichlorobenzene diesel fuel 1 000 ppm or 0 1 1 000 ppm or 0 1 If additional dilutions of the soil extract are made to detect soil PCB concentrations greater than 10 ppm these interferences are diminished in direct proportions to the dilution made Z 00254 1 Rev 4 10 00 Range of Detection The PCB RaPID Assay has a range of detection in soil of 500 ppb to 10 ppm as Aroclor 1254 when used in conjunction with the SDI Sample Extraction Kit Recovery PCB recoveries will vary depending on soil type retention mechanism solvent and extraction apparatus used length of extraction period and levels of potentially interfering substances in the soil
19. t is a screening test only Sampling error may significantly affect testing reliability Adequate sample number and distribution are the responsibility of the analyst RaPID Assay PCB Test Kit 9 Ordering Information To obtain part numbers and pricing for individual items in the Accessory Kit contact SDI at the number below Ordering Technical Assistance Should you have any questions regarding this procedure prior to analysis contact Technical Service to avoid costly mistakes To Place an Order or Receive Technical Assistance please call Strategic Diagnostics Inc at Call toll free 800 544 8881 Or 302 456 6789 Phone 302 456 6782 Fax Web site Www sdix com E mail techservice sdix com General Limited Warranty SDI s products are manufactured under strict quality control guidelines and are warranted to be free from defects in materials and workmanship New instruments and related non expendable items are warranted for one year from date of shipment against defective materials or workmanship under normal use and service Warranty obligation is limited to repair or replacement of the defective product or to refund of the purchase price at the discretion of SDI Other warranties express or implied are disclaimed SDI s liability under any warranty claim shall not exceed the refund of the purchase price paid by the customer Under no circumstances shall SDI be liable for special indirect or consequential damages Sa
20. ter decanting the particles are washed with Washing Solution The presence of PCB is detected by adding the enzyme substrate hydrogen peroxide and the chromogen 3 3 5 5 tetramethylbenzidine The enzyme labeled PCB analog bound to the PCB antibody catalyzes the conversion of the substrate chromogen mixture to a colored product After an incubation period the reaction is stopped and stabilized by the addition of acid Since the labeled PCB conjugate was in competition with the unlabeled PCB sample for the antibody sites the color developed is inversely proportional to the concentration of PCB in the sample NOTE Color development is inversely proportional to the PCB concentration Darker color lower concentration Lighter color higher concentration The determination of the PCB level in an unknown sample is interpreted relative to the standard curve generated from kit standards after reading with a spectrophotometer Performance Characteristics The PCB RaPID Assay will detect different PCB Aroclors to different degrees Refer to the table below for data on several of these The PCB RaPID Assay kit provides screening results As with any analytical technique GC HPLC etc positive results requiring some action should be confirmed by an alternative method The PCB RaPID Assay immunoassay test does not differentiate between PCB and other related compounds The table below shows compounds at the method
21. tion below the minimum detection level of the test kit Results must be reported as lt 0 5 ppb or Standard 1 multiplied by the dilution factor NOTE Any samples with concentrations determined to be lower than Standard 1 the limit of quantitation must be reported as lt 0 5 or Standard 1 multiplied by the dilution factor Quantitation is not possible below this standard as this is outside the linear range of the assay d Similarly samples with a hi next to a listed concentration have an estimated concentration higher than Standard 3 and must be reported as gt 10 ppb or Standard 3 multiplied by the dilution factor NOTE In order to determine the concentration of samples with concentrations greater than Standard 3 they must be subjected to repeat testing using a diluted sample A ten fold or greater dilution of the sample is recommended with an appropriate amount of PCB diluent This additional dilution must then be taken into account when calculating the concentration Please contact technical support for assistance in performing dilutions Photometric Interpretation Using Other Photometers Other photometers may also be used to interpret results obtained from the RPA I photometer It is important that the photometer be able to read absorbance at 450nm and that the instrument can read at a 1 mL fill volume Absorbances obtained from other spectrophotometers reading at 450 nm may be used to manually calculate sam
22. ts or test tubes from one test kit with reagents or test tubes from a different test kit Do not mix reagents from kits of different lot numbers Use approved methodologies to confirm any positive results Do not under any circumstances attempt to disassemble the base of the magnetic rack Magnets will be violently attracted to each other Adequate sample number and distribution are the responsibility of the analyst The photometer provided in the accessory kit requires electricity and comes with a 110V adapter Adapters for 220V are available Do not attempt to operate with a car adaptor Do not expose color solution to direct sunlight Do not dilute or adulterate test reagents or use samples not called for in the test procedure this may give inaccurate results Tightly recap the standard vials when not in use to prevent evaporative loss Materials Provided Antibody Coupled Paramagnetic Particles in buffered saline containing preservative and stabilizers 30 test kit one 20 mL vial 100 test kit one 65 mL vial Enzyme Conjugate 30 test kit one 10 mL vial 100 test kit one 35 mL vial Standards Three concentrations 0 25 1 0 and 5 0 ppb of PCB standards as Aroclor 1254 in buffered saline RaPID Assay PCB Test Kit containing preservative and stabilizers are supplied Each vial contains 4 mL e Control A concentration approximately 3 ppb of PCB as Aroclor 1254 in buffered saline containing p
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