Ar6 User Guide - Reichert Technologies: Analytical Instruments
Contents
1. 10 C 40 C in channel 1 to a reference temperature reading usually 20 C This can be used in lieu of controlling the temperature of the prism and sample with an external water bath Temperature correction or control is necessary because refractive index varies inversely with temperature A typical temperature coefficient for an aqueous solution is 0 00021 C For an organic liquid it is typically 0 0004 C However itis awaysnecessary to allow yoursample temperature to equilibrate so that the AR6 can properly correct for temperature Theautomatictemperaiure compensation modes in channel 1 arebasedonthe temperature coefficients n 1 ofsucrose perthe ICUMSA scales Whentaking readings inthe nD TC refractive index Temperature Compensated mode these coefficients may not be valid depending on the solution 9 0 WATER BATH ATTACHMENT There may be testing applications that require that the prism assembly temperature be maintained above or below ambient temperature Commercial water circulators with heating cooling or combined capabilities are generally available from instrument supply dealers Check with your Reichert customer service representative for a suggested list of suppliers If a water bath attachment is used care should be taken to avoid exposing the prism assembly to thermal shock The ambient prism temperature should be raised and lowered by gradually increasing or decreasing the temperature of t2. Reichert AR6 SERIES Automatic Refractometer Reichert REICHERT AR6 SERIES Refractometers Electrical Receptacle 100 to 240VAC 50 60 Cycle On Off Fuse 5 x 20 5A T250 1 2 Instruction Manual TableofContents Section Page 1 0 INTRODUCTION aan 3 2 0 UNPACKING Een 3 90 SETUP ee ae 3 3 1 Electrical Considerations Equipment RANGE entrante is 3 RS 232c Parallel Port e 4 DB9 Female DB25 Female 4 0 COMPONENTS nne 4 5 0 OPERATING INSTRUCTIONS 4 5 1 Preparing the Prism Surface for Figure 1 SAMPES nn 8 5 2 Applying the Sample and Taking Readings easicscnssetanedpntesvoceanctnntseseocstnceneteisseeneanee 8 6 0 DIAGNOSTICS escciaci niniosinsinnccici sinia n 8 7 0 CHANNELS en en 8 8 0 AUTOMATIC TEMPERATURE COMPENSATION een 8 9 0 WATERBATHATTACHMENT 9 10 0 CARE AND CLEANING 9 10 1 Care of Prism Surface 4044 9 Figure 2 11 0 LAMP REPLACEMENT 9 12 0 RS232C SERIAL PORT 9 13 0 PRINTER OUTPUT ee 9 14 0 SPECIFICATIONS e 10 15 0 ACCESSORIES ann nenn 10 Explanation of Warning Symbols IN CAUTION Risk of electric shock CAUTION Replace with same type and rating fuse 5x20 5A T250 ZN CAUTION Refer to accompanying documents Figure 4 Please notethatarefraciometerisahigh precision optical instrument Itis absolutely necessary to properly maintain the refractometerto obtain
3. 14 A successful calibration will bring up screen 15 Repeat the procedure for the rest of your calibration standards NOTE The calibration standards must be sequential Inother words the index ofwateris lt sta 1 lt std 2 lt std 3 lt std 4 TheAR6 will not sort the standards if you scramble their order and the accuracy of your readings will diminish 51 PreparingthePrismSurfaceforSamples Because a refractometer is an optical instrument itis critical to thoroughly clean the sample measurementarea Any residue could result in erroneous readings or error messages Clean the prism surface and sample well with diluted soapy water or alcohol followed by a distilled water rinse before applying a sample Wipe clean with a soft cloth 5 2 Applying the Sample Taking Readings and Shadowline Dis play Placethesample onthe prism surface using apipetor applicator Make sure the entire prism surface is covered Figure 4 Optimum readings are obtained if enough sample is placed in the well to cover a millimeter or two of the walls of the sample well Closethe coverandpress the READ key The REICHERT AR6 will display the reading in the selected mode lfyouchangemodes before taking anew reading the instumentwill use data from the current reading to produce a new display inthe newly selected mode Forexample ifyouwanta reading in np foHowedby Solids using one measurementofthe sample operate the REICHERT AR6 in the np mode
4. 20 C Press MODE to continue Channel 1 Screen 10 Sucrose ICUMSA Calibration Standard Index Value 1 51416 Use ti gt to change setting Press MODE to continue Screen 11 Calibration Standard Temperature Coefficient 0 000412 Use t4 to change setting Press MODE to continue Screen 12 Calibration Standard Reference Temperature 25 00 Use ti gt to change setting Press MODE to continue Screen 13 Add calibration standard to sample well Allow to equilibrate Press CALIBRATE or MODE to go back Screen 14 READ or CALIBRATE buttons when this option is used Interval Timer Default Setting Off Thisoptionallowstheinstrumenttotakereadings at definable intervals The intervalis setin HH MM SS format Once activated and defined the instrumentwil continually take readings until a lock outerroroccurs such as alack of sample or until SETUP is pressed and the option isdisabled The interval timer will beginits countdownto the nextreadingoncethecurrentreadingis finished andthe resultis displayed This featureis mutually exclusive of the Delay Timer and Auto Equilloration feature Auto Equilibration Default Setting Off Auto equilibration is afeature which will automatically detectwhen the sample and prism havereacheda thermal equilibrium state Thermal equilibrium is essential for accurate measurement of refractive index and thus Solids This feature may be setfrom 1
5. index standard Once changed the low refractive index standard will become the default value for the first point of all calibrations In routine use a single point water or a 2 point calibration water and a refractive index standard at the high end of the scale will provide the required accuracy A refractive index standard oil part 13K41330 nD 1 51416 25 C tempera ture coefficient 0 000412 is included with the instrument The 3 4 and 5 point calibrations allow the user to precisely calibrate the instrument to standards near the refractive index range where measure ments are being made To obtain certified standards contact Reichert Microsystems Inc Pressthe SETUP key screen 7 Use the right arrow to position the cursor at the calibrate routine position Use the up arrow to change the calibrate routine to 2 3 4 or 5 point You may also want to set the timer for 15 or 30 seconds to ensure your calibration standards reach thermal equilibrium with the prism Press SETUP again Screen 8 will appear Cleanthe prism andpress INITIATE Whenthe initialization is complete the water pointscreen 9 will appear Atthis point you can choose betweendoinga solids oran index calibration pressing MODE willtoggle this Place yourdistiledwatersample onthe prism allowitto reach thermal equilibrium unless you setthe timer and press CALIBRATE Ifyou are using alow refractive index standard forthis point press SETUP
6. oralcoholbefore applyinga sample Wipe clean with a softcloth rinse with distilled waterandwipedry between samples CAUTION Theprsmsurfaceandsample wellmustbe thoroughly cleaned after each sample is removed Any residue can affect the accuracy of the readings Avoid using scented tissue tissue with lotion cotton or any other material which may leave a residue on the prism surface when cleaning It Placethe sample onthe prism using a pipette or applicator Make sure the entire prism surface is covered Close the cover allowthe sampleto come totempera ture equilibrium and press READ The AR6 will display the reading in the chosen mode see Screen 6 Temperature Effects on Measurements Measurements taken using refractometry arevery sensitive to subtle changes in temperature Take this into consideration when using the AR6 refracto meter Samples with a significantly different temperature from the prism will take a longer time to equilibrate NOTE If a water bath circulator is attached to the AR6 there may be a difference between the temperatures displayed on the refractometer and that measured in the circulator due to heat loss through the tubing and heat gain from the instrument The temperature indicated on the AR6 should be used if temperature corrections are required 4 Multipoint Calibration The AR6 may be calibrated with up to 4 standards plus water If necessary the water point may be changed to a low refractive
7. shadowline display press CALI BRATE and MODE simultaneously GODIAGNOSTICS Thefollowing screens describe potential errorsthatcan oocurwhenoperalingthe AR60O High Sample screen Thesamplehas arefractive index exceeding the 1 52000 operating range limit see Screen 16 Low Sample screen The sample has a refractive index below the 1 32000 operating range limit See Screen 18 Water Calibration screen This screen willbe displayed whenthe READ keyis pressed before a water calibration Clean the prism caliorate with water and rereadthesample see Screen 19 LampisnotAligned screen This screen may appear if asample has been lefton the prism Thoroughly clean anddry the prism surface Ifthe message persists the lampmay needre algnment Note the direcionto move thelampandre centerthe lamp Alignmentisdone with theinstrumentrunning Use ametal screwdriverto move thelampduetoits hightemperature Exercise care to avoid breakingthe lamp Wear eye protection incasethe lamp shatters Followthe on screen instruction to initiate and recalibrate the AR6 see Screen 20 Replace Lamp screen ThelampisbumedoutReplace the lamp following instructions in section 11 0 LAMP REPLACEMENT see screen 21 Could Not Take Proper Reading screen This screen willbe displayed ifthe lamp intensity has changed since theinitalization procedure This canbecausedby normal aging ofthe lamporlarge line voltage f
8. through the serial port interface by using any standard communications program such as Terminal or Hyperterminal Reading output through the serial port may be formatted to retrieve virtually any data in any order desired To format the serial string output type the letters help sf Brief instructions will appear To set the serial format type the letters sf followed by the letters for the output options desired The default setting is GFA Numerous additional commands are available through the serial port Ask a Reichert representative for a complete list of commands 130PRINTEROUTPUT The REICHERT ARE refractometer is compatible with an external printer The parameters included in the parallel port data output string correspond to the serial port data output string To select parameters for output set the serial port output format as in Section 12 0 The output is available at the DB 25P type connector located on the rear panel of the REICHERT AR6 Figure 1 Communication is by a 7 bit parallel bus inter face with synchronous operation Contact your Reichert customer service representative for more information about connecting a printer to your REICHERT AR6 refractometer 14 0 SPECIFICATIONS Models AR60 AR600 Illumination 10 Watt Tungsten Halogen with 589nm bandpass filter Display 5 1 4 X 3 134mm x 76mm graphical LCD Range Channel 1 Dissolved Solids O to 90 Solids as sucrose per ICUMSA
9. to 10 This translates into stability oftemperature in 0 01 degree increments A higher number represents alooser tolerance alowernumberreoresents atighter tolerance When this feature is activated anda READ is initiated the instumentwill begin monitoring the temperature A graonwillbe displayed ofthe dynamicwelltemperature Once the temperature stabilizes the instumentwil automatically take the reading Sample Indexing Default Setting Off Sample readingscan be easily labeled andnumbered To activate selectthe on setting and press setup A display will feature a section to enter the desired label alphabetic numeric and or symbols a sectionto define reading increments andan instruction section Oncethe labelling schemeis set follow the instructions to see a preview ofthe firstten readings Inthis screen you willbe offered options to see the next 10 labels re define the labeling orexitandbegin readings Calibration Tolerance Default Setting 1 Themostcommon cause of calibration difficulties is tne condition ofthe prism A refractometer is a high precision optical instrument Thoroughly dean the prismwith soap and water oralcohol followed by arinsewith cistilled water Wipe dry with asofttissue or cloth Ifyou are still having difficulty increase the value ofthe calibration tolerance Thiswill loosen up the insttuments tolerance for the calibration standard Itis importantto verify that the values ofth
10. water is recommended for removing Sugar residues and methanol for oil based samples followed by a water cleaning Use only diluted neutral detergents to clean the prism Do not use any metal or sharp pointed objects to clean or pry loose the prism seal or residue on the sample well 11 0 LAMP REPLACEMENT WARNING Unplug the instrument power cord before attempting to replace the illuminator lamp Remove the old lamp and replace it with Catalog No 13105040 The contacts of the bulb must be cleaned with a soft emery cloth before replace ment Be sure that no fingerprints are left on the surface of the replacement bulb 12 0 RS232C The REICHERT AR6 refractometer includes a serial communications port accessible from the rear panel The port has a DB 9 pin female connector Output data is comma delimited in standard ASCII code and consists of the channel number e g CH1 the channel identifier e g Brix for channel 1 the mode e g Solids TC20 for a percent solids scale temperature compensated to 20 degrees centigrade the actual reading e g 10 62 and the actual measured sample temperature e g 22 67C This port will support custom channel programming using standard communication Software Do not use a Null Modem cable Communications settings are 8 Data Bits No Parity 1 Stop Bit Flow Control Xon Xoff Baud Rate settings are described in section 5 2 The AR6 is capable of being controlled from a computer
11. 0 Set Up Calibration Calibration Calibration _ Routine Tolerance History 5 Point Interval Delay Timer to choose option 4 to choose setting To exit press lt SETUP gt Screen 2 Enter new password 82 Use 4 to change value Be sure to write this down you can not use setup without knowing this password Entering 000 will cancel setting and turn password protection off Press SETUP when done Screen 3 Initialization complete Use Mode key to Select Mode current mode INDEX TC Add distilled water to sample well Allow to equilibrate and press CALIBRATE Well Temp 248C Screen 4 1 33299 inbex 1C Reference Temperature 20 C Well Temp Channel 1 Sucrose ICUMSA 24 7 C Screen 5 8 84 soups tc Reference Temperature 20 C Well Temp Sucrose ICUMSA 26 0 C Channel 1 Screen 6 AR600 Set Up Calibration Calibration Calibration Routine Tolerance History 5 Point interval Delay Timer gt to choose option 4 to choose setting To exit press lt SETUP gt Screen 7 5 Sample Well The sample well is made of stainless steel In the center of the sample well is a sapphire prism surface upon which samples are placed for measurement It is important that the sample covers the prism surface for an accurate reading Control Keys Functions 1 INITIATE Key Press this key to initiate the REICHERT AR6 prior to calibrating with water Remember that the instrument
12. Figures 2and 3to locateihe components deseribedbelow 1 Instrument Display The instrumentdisplay is positioned on the top panel of the REICHERT ARB It digitally displays measurement values error messages and operator instructions 2 Controls The controls positioned tothe right ofthe instrument display are used to operate the REICHERT ARE All five controls are soft touch membrane keys They are labeled INITIATE MODE CALIBRATE READ and SETUP These controls will be used to take measurements of test samples The READ MODE INITIATE and CALIBRATE keys can also serve a dual function In the SET UP screen see section 5 0 OPERAT ING INSTRUCTIONS these keys are used to move a cursor The direction of move ment is indicated by the arrow near the key 3 Water Bath Attachment Ports Two inlet outlet ports located on the leftside ofthe REICHERT AR6 permit attachment of an optional water bath 4 Sample Well Cover The circular hingedsample well cover is located inthe middle ofthe refractometer s horizontal top panel Itis importantthatthe sample well coverbeclosedwhenameasurementis being takento eliminate or minimize stray lightand evaporation effects Keepingthe cover closed when the instrumentis notin use will preventdamage andcontaminationtothe sample well andthe prism surface Reichert Automatic Refractometer AR600 Version 12 6 Allow to warm up and press INITIATE or for Set Up press SETUP Screen I AR60
13. Refractive Index 1 32000to 1 52000nD nD Sodium D Line Refractive Index Readability AR60 0 1 Solids 0 0001nD AR600 0 01 Solids0 00001nD Precision AR60 0 1 Solids 0 0001nD AR600 0 01 Solids 0 00001nD Refractive Index Standard Oils 0 00002 Typical clear samples Solids Temperature Compensated as sucrose 0 02 Temperature Compensation Range 10 45 T per ICUMSA tables Channel 1 User Definable Channels2 15 Temperature CompensationAccuracy 0 1 Sample Types Transparent TranslucentorOpaque Dimensions 111 4W x 16 L x 61 4 H 28 5cm x 40 6cm x 15 9cm NetWelght16lbs 7 3kg Sample Temperature Control Heat cool exchanger surrounding prism permits temperature control by an extemal waterbath circulator from 10 C 60 TC Prism Assembly Stainless Steel synthetic Sapphire sealed with solvent resistantepoxy Data Output 25 pin parallel port 9 pin RS232C Baud Rate 1200 to 19 200 user settable 10 Calibration 1 point Water or low index standard 2 5 point Water or low index standard and Refractive Index or Brix standard Channels Channel 1 ICUMSA Brix scale Channels 2 15 User programmable Sample Scans 8 256 user settable Read Countdown Timer 0 4 min adjustable in 15 second intervals Password 3 digit user settable numeric password The precision and accuracy of Refractive Index measurements is dependent on operator skill the laboratory environment temperature c
14. Then press the MODE key until Solids or Solids TC registers on the display A Solids reading will automatically be displayed If the READ key is pressed the AR6 will take an entirely new measure ment In addition the instrument now has the ability to graphically display the shadowline via the Linear Scanned Array output This allows the user to see whatthe refractometer sees This feature may be operated after any reading by pressing the CALIBRATE and MODE keys simultaneously The refractometer willtnhen generate anddisplay agraph ofthe Linear Scanned Array Output CCN 1150 xX 972 Y 24831 See example below Two lines will appear The rainbow shaped line is the Reference Scan The line which begins near the bottom of the screen and moves upward to intersect the Reference Scan is the Sample Reference Scan Also shown will be the CCN This is the Crossing Cell Number which is the exact cell on the array which the edge of the shadowline falls on At this point a box will be displayed This box may be repositioned anywhere on the screen Pressing SET UP will zoom in on this boxed area Pressing SET UP again will return to the full graph view The X and Y values are also displayed These are simply the location of the box on the screen The line for the reference scan may be toggled on and off by pressing READ and INITIATE simultaneously in the full graph display To exit the
15. and follow the on screen instructions to enter the refractive index temperature coefficient and reference temperature of your standard The AR6 will take readings until it achieves three consecutive identical readings or fails to do so in ten tries A failure will prompt you to clean the prism re initialize the unit and then reapply the sample the same holds true for all calibration points A successful calibration will bring up screen 10 At this time pressing MODE will bring up screen 11 This unithas the capability to calibrate with 1 2 3 4or5 points Naturally the more points you use the more accurate your readings willbe The calibration standards are NOT mutually exclusive in other words the points used in a 3 point calibration will all be contained in a 5 point calibration The following chart should clarify this Water Std 1 Std 2 Std 3 Std 4 1 PtCal o 2 PtCal o o 3 PtCal O o 4 PtCal O o o o 5 Pt Cal o O o o O USED NOTUSED Usethe up amp down arrowkeys to adjustthe index or Solids ofyour calibration standard Holding the key down willcause the numbers to change faster When itis correct press MODE anddothe same forthe temperature coefficentandthen the datumtemperature ofthestandard screens 12 and 13 Youcancontinueto press MODE to retum to index adjustment etc When you have the numbers correct apply the sample allowto equilibrate and press CALIBRATE screen
16. e calibration standards are equivalentto those programmedinto the instrument Be aware that every solution has aunique refractive index and temperature coefficient at a specific reference temperature Also note that increasing the magnitude of the calibration tolerance can decrease the accuracy of the instrument This option should be the last choice for calibration difficulty The calibra tion tolerance can be set from 1 represent ing the most stringent standards the factory standards used for calibrating with distilled deionized water and NIST certified calibra tion solutions to 50 the loosest standard Invert Display Default Setting Off Thedisplay screen may be displayed in eitheradark background with light lettering or a light background with dark lettering Instrument performance is not affected by this feature Language Default Setting English Screens can bedisplayed in English Spanish French or German Note thatthe customchannelnamesandunit definitions will appear as entered in the custom channel program Time and Date The Reichert AR6 can track the clock time in either standard 12 hour am pm time or military time The AR6 is Year 2000 complient The date may be set in Month Day Year format Display Time Default Setting Off Activating this function will display the exacttime each reading istaken Thistime will remain until another readingistaken Restore Defaults Default Setting Off This allows t
17. he circulating water The REICHERT AR600 s automatic temperature compensation feature is designed to provide precise measurements in the 10 45 C temperature range Channel 1 Connections between the instrument and the circulator should be 1 4 635cm flexible tubing Observe the operating instructions of the water bath manufacturer 10 0 CARE AND CLEANING The REICHERT AR is a precision optical instrument designed for years of trouble free operation The interior components are protected from dust spills and other damage The stainless steel refractive prism assembly is sealed with corrosion resistant epoxy Theuseofstrong solvents suchas N N DimethylformamideorN N Nimethylacetamide cresols phenols and othertaracids shouldbe avoided Tetrahydrofuranorsomelacauerthinnersandtheir componentscanalsodeteroratetheprismsealovera long period of time or when applied at elevated temperatures Allnon opticalcomponentscanbecleanedwithsoapand wateror methanol Avoid usingall other harshsolventsor acetone basedproductswhendeaning 10 1 CareofPrism Surface The prism surface shouldbe cleaned immediately after use The sample shouldbe wiped off with adry tissue followed by athorough cleaning with soap andwater or methanolandihen distilled water Thesapphire prismis very hardandhas exceHent scratch and staining resistance Strong acids orbases however should notbe measured with this instrument norused as cleaning agents Warm
18. he userto restore all settings to the factory defaults when SETUP is pressed again Thisdoes not erase custom channels or calibration history Calibration History Default Setting Off Switching this setting to on will display the previous 512 calibrations Some parameters extend beyond the right hand portion ofthe display Tosee these simply press the MODE button untilthe parameter desiredis visible The calioration history maybedownloadedto acomputer through thesenal portly use ofastandard communica tons programandenteringthecommand calhis Precisi Default Setting On ON Instrument reads refractive indexto the 5th decimal place 0 00001 and Solids tothe 2nd decimal place Calibration Standard Completed 1 51416 innex TcC Reference Temperature 25 00 C Press MODE to continue Refractive index of sample exceeds AR600 limits Well Temp 249C Sereen 16 Cannot perform water calibration check water standard Well Temp 26 3 C Sereen 17 Refractive index of sample is below AR600 limits Screen 18 Water calibration is required before operating instrument or after an initialization Apply water and press CALIBRATE Well Temp 26 3 C Screen 19 Lamp is not aligned properly Adjust lamp up until TILT approaches Zero TILT 10 or prism surface may not be clean Press MODE to continue Screen 20 Replace lamp consult instruction manual Screen 21 0 01 OFF I
19. he words Initiating Please Wait will appear During the initiating sequence the lamp brightnessissetto an optimum level Whenthe insttumenthas finishedinitiating the Initialization Complete screen screen4 wil appear Followthe onscreen instructions to add distilled water to thesample well allow the temperature to equilibrate and press CALIBRATE The instrumentwil display a refractive index reading between 1 33400 prism temperature of 10 C and 1 33059 prism temperature of 40 A typical reading wouldbe 1 33299 atareference temperature of 20 C seescreen5 Pressthe SETUP key to exitthis screen Atthis time the MODE key canbe used to display the reading in any ofthe 4 instumentmodes Index The nontemperature compensated refractive index reading Index TC Thetemperature compensated refractive index reading Fordistiledwaterthis value shouldbe 1 33299 0 00002 Solids The non temperature compen sated Solids scale Y Sucrose or Brix The instrument could not take a proper reading Either the sample is out of range OR Solids TC The temperature compen The lamp intensity has changed sated Solids scale This reading should be 0 0 t 02 for distilled water Piease check the lamp and re initiate Well Temp 26 3 C Select the mode in which you want to read Screen 22 samples The AR6 is now ready to read samples Clean the prism surface and sample well with distilled water diluted soapy water
20. luctuations Check tobesure the lampis illuminated tum the AR60O off and back on follow the on screen instruction to initiate and caliorate instrument see Screen 22 Ifthe condition persists change the lamp If it continues with a new lamp a line voltage conditioner may be required Alternately the AR60O could be plugged into a separate line source preferably a source without electrical appliances which have large current draws e g motors compressors heaters etc 7 0 CHANNELS Channel one 1 on the REICHERT ARE is pre programmed at the factory with the Sucrose or Brix scale per the International Commission for Uniform Methods of Sugar Analysis ICUMSA Upto 14 additional customchannels may be programmediusing your own refractive index temperature Solids data Access to the channels is fromthe SET UP screen Custom channels may also include definitions for units of measure such as specific gravity protein g ml etc The unitof measure labels arecompletely definable and upto 20 characters canbe used Results may be displayed inasmany decimal places asdesired Toleammoreaboutcustom channelprogamming request Custom Channel Generationforthe Automatic Refractometer Line froma Reichertreoresentative Be suretoinform the representative that you are using software version 12 6 80 AUTOMATICTEVPERATURE COMPENSATION Automatic Temperature Compensation corrects readings taken over a range of temperatures e g
21. nstrument reads refractive index to the 4th decimal 0 0001 and Solids to the 1st decimal 0 1 place Password Default Setting Off Password protection canbe usedto secure options once theyareset Tuming password ONand pressing SETUP displays the Enter New Password screen see Screen 3 Usethe arrowkeys to enter a3 digitnumerical pass word Entering 000 cancels the setting andtums the password protection off Press SETUP to exitthe Enter New Password screen second screen confirming your choice of password will appear Press SETUP tocancel or READtoacceptinenewpassword Onceapassword isentered the instumentwil promptthe userforihe password each time the Set Up screen is accessed Sound Default Setting Off ON Chime sounds whenkey is pressed ora reading or calibration is displayed OFF Soundistumed off Oncealloperational parameters have been setusing the Set Upscreen press SETUP to retum to the start screen Screen 1 3 Calibration and Sample Reading NOTE Theseinstructions assume the sample wellis cleanandaty priorto starting The sample wellshould alsobe completely deanedbetween samples Cleanwith distilled water soapy water meihanolorisopropylalcohol Dry with a soft cloth Theseinstructions assumethe one pointcalibration default waschosen insetup See Multipoint Calibration for instructions forthe muttipointcalibration Fromthestartscreen press INITIATE The instrument screen will blank andt
22. ontrol the accuracy of calibration standards chemical stability of the sample viscosity of the sample and concentration of the sample A product demonstration is suggested to validate the true precision and accuracy of these instruments in your laboratory 15 0 ACCESSORIES Catalog No 13106100 Flow cell with temperature control The flow cell allows samples to be pumped to the prism surface Typically a peristaltic pump with a flow rate of approximately 10 15 ml min is used Use of the flow cell generally increases instrument precision and eliminates errors due to an unclean prism surface Use of the flow cell also allows the user to pre temperature equilibrate extremely hot or cold samples Ports are provided for circulation of a controlled temperature fluid Catalog No 13106200 Funnel Flow Cell with temperature control This offers the same benefits as the flow cell but eliminates the need for a pumping device 13106200 includes the flow cell funnel funnel attachment and tubing Catalog No 13106300 Sample press with temperature control The sample press creates an enclosed sample volume by contacting the top of sample and the prism well lt is useful for decreasing equilibration times of very viscous or hot or cold samples 11 Reichert Analytical Instruments Reichert Inc 3374 Walden Ave Depew NY 14043 USA Toll Free 888 849 8955 Phone 716 686 4500 Fax 716 686 4555 email ai reichert com ww
23. should have reached room temperature before it is initiated 2 SETUPKey Press this key to access the Set Up screen for options Screen 2 3 MODE Key e Refractive index nD TemperatureoompensatednDassucrose Brix in Channel 1 Solids as sucrose Brix Channel 1 Temperature compensated Solids as sucrose Brix inChanne 1 4 READKey Presstoobtain readings ofasample 5 CALIBRATE Key Press to calibrate the REICHERT ARE 500PERATINGINSTRUCTIONS 1 StartUp Plug inthe instrument Make sure the samplewellis Clean anddry andthe coveris inthe down position Tum theinstument on The switch is located onthe rear panel see Fig 1 Ifthe instumentdoes notpower up check for loose or blown fuses Fuses are located onthe rear panel The REICHERT AR6 will prompt you through preliminary initialization and calibration steps Additionally a Set Up screen is available to access and change operating parameters 2 SetUp When the instrumentis firsttumedon the ReichertLogo and Model numberwill appear Screen 1 Alsoshownis the software version e g 12 6 This screen instructs the userto Allow to Warm Upand Press Initiate Under normal circumstances withtheinstrumentatroom temperature a 15 minute warm upis sufficientbefore pressing the Initiate button Pressing Initiate continues theinstumentoperationsequence Pressingthe SETUP button accesses the Set Up screen Once the instrument s parame
24. strument Neveruseanadapterthatmay nteruotineprotecivegrounding Theinstrumentisequipoedwithaventiation entranceintne baseandanexhaustfanattherear keeotneseooenings freeofdebrisorobstruciion 31 Bectical Considerations EquipmentRatings REICHERT AR6 Refractometer incorporates an auto sensing switching AC power supply IMPORTANT Beforeatiempingtosetupandoperateihe CAUTION Risk of electrical shock Removal of the cover of the REICHERT AR6 exposes hazardous voltages which if contacted could cause injury or death Please refer servicing to a qualified service technician Supply Voltage Range 100 to 240V AC 50 60 HZ Input power 40W PMAX REICHERT AR6 allow the instrument to reach a room temperature of at east 15 T 59 F Attach power cord to the electric receptacle on the rear panel of the REICHERT AR6 see Figure 1 Turn the instrument on by activating the On Off switch located on the rear panel NOTE Theinsttumentshoukdlbealowediowarmupfor15 RangeofEnvironmental Conditions Indooruseonly Temperatures 10T45T Maxrelative humidity 80 fortemperatures upto 31 Cdecreasinglinearly to 50 relative humidity at40C Installation category Il overvoltage category Pollutondegree2 Altitude upto 2 000 meters 32Fuses For additional safety all REICHERT AR6 Refracto meters use two line fuses both accessible from the rear panel Replace with 0 5 amp Time lag T or Blue 40COMPONENTS Referto
25. ters are set correctly the Set Up screencanbebypassed This screencanalsobe bypassed ifyou choose to use the defaultparameters Press SETUP toacoesstheSetUpscreen Screen2 This is the AR6 Set Up screen Use the right left arrow keys to select the set up parameter you wish to change Use the up down arrows to make the changes The following is a brief description of the parameters and available options Baud Rate Default 19 200 bps Sets the baudrate ofthe RS232C serial port Options are 1200 2400 4800 9600 and 19200bps Calibrate Routine Default Setting 1 point Selects 1 point2 3 4or5 pointcalibration The default Calibration isa 1 pointwater or low refractive index standard calibration Upto5 points may be selected Contact Reichertfor additional certified calibration liquids Temp Disp Default Setting ON ON Instrumentscreen continually shows actual prism temperature OFF Temperatureis notdisplayed continually butshown onlywhen READ or CALIBRATE ispressed Channel Default Setting Channel 1 Sucrose Options 1 15 Channel 1 isthe default Bnix or Intemational Commission for Uniform Methods of Sugar Analysis sucrose scale Channels 2 15 areuser programmablechannels Fordirecionsandan explanation ofthe necessity of custom channel generation please requestthe Technical Bulletin Custom Channel Generation forthe Automatic Refractometer Line Be sure to notify the representative thatyourinstrumentu
26. thebestpossible accuracy This includes thoroughly cleaning the prism and well after each sample routinely calibrating the instrument and replacing the lamp as necessary 1 0 INTRODUCTION This manual describes the proper use and operation of the REICHERT AR6 Refractome ter The REICHERT AR6 is an automatic bench top refractometer with digital readout It measures solids Brix solids Brix temperature compensated refractive index and refractive index temperature compen sated Sucrose It can be used for applica tions in the medical pharmaceutical industrial fluids quality control chemicals plastics petrochemicals and food beverage industries 20UNPACKING Carefully remove the REICHERT AR6 from the packing box The following accessories have been included with the instrument Powveroord Dustoover catalogNo 131050043 eInstucionmanual 13K41330caliorationol Donotdiscardithe packing materials Ifforany reasonit becomes necessary to store or ship your REICHERT AR6 Refractometer use the packing materials for protection against damage 30SET The REICHERT MODEL AR6Refractometer requires no assembly The instrument should be positioned on a bench top Care should be taken to place the instrument in an environ ment with a stable temperature i e away from an air conditioner or water bath exhaust draft and minimal vibration Asasafety precautionthepowercordhasbeengroundedto thechassisofthein
27. tilizes software version 12 6 In Channel 1 the refractive index readingis convertedto Solids as sucrose Brix when readings are taken in the Solids or Solids TC temperature compen sated to 20 C modes Inthe refractive index TC Mode thereadingistemperaturecompensatedioa20 C readingbasedonthetemperaturecoefficientofsucrose Scans Default Setting 128 Setsthnenumberoftimesthelinear aray is scannedto take areading Options are 8 16 32 64 128 256 This number reoresents the number oftimes the refractometer actually analyzes the sample everytime a read is performed Thehigherthe number ofscans the more precise the resultwill be A higher number of scans will require longer to accomplish Typically 256 scans will take approximately 14 seconds whereas 8scanswilltake 4 seconds Any time the number ofscans is changed the instrumentmustbe re initiated and re calilorated Delay Timer Default Setting O seconds Sets adelay timerfromthetimethe READ or CALIBRATE buttons are pushed untilthe actual reading isteken This parametercanbesetirom 15 seconds to 4 minutes in 15secondintervals A countdowntimeris displayed on screen after pushing the Clean prism and press INITIATE Multipoint Calibration Water Point Use MODE to Select Mode Current mode INDEX TC Add Distilled Water to Sample Well Allow to equilibrate and press CALIBRATE Water Calibration Complete 1 33299 inbex 1C Reference Temperature
28. w reichert com Reichert GmbH Hubertusstrasse 4 D 82229 Seefeld Germany Tel 49 8152 993530 ISO 9001 Certified Reichert 2003 Reichert Inc Depew NY USA 10600 100 Rev H
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