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1. Max Number of Rotor RCF Tubes x Rotor Selection Max x 9 Nominal Manual Rotor Profile and Description Code RPM at max Capacity Number FX6100 Fixed Angle Fmax 98 0 mm FX6100 10 200 11 400 6 x 100 mL GX TB 005 SX4750 Swinging Bucket Tube and bottle buckets SX4750 3 750 3 270 4x 750 mL GX TB 003 Imax 207 8 mm Multiwell plate carriers SX4750u 3750 2 885 4 x 96 mL Imax 183 2 mm SX4750A Swinging Bucket ARIES Tube and bottle buckets SX4750A 3750 3 270 4x 750 mL GX TB 004 Tmax 207 8 mm Qi Multiwell plate carriers SX4750A 3750 2 885 4 x 96 mL Tmax 183 2 mm Maximum speeds are based on a solution density of 1 2 g mL At upper temperature and humidity ambient conditions swinging bucket rotor speed may require reduction t Relative Centrifugal Field RCF is the ratio of the centrifugal acceleration at a specified radius and speed r to the standard acceleration of gravity g according to the following formula RCF 1 8 where r is the radius in millimeters is the angular velocity in radians per second 2 7 RPM 60 and g is the standard acceleration of gravity 9807 mm s After substitution RCF 1 12r RPM 1000 2 Installation Preinstallation requirements have been sent prior to shipment of your centrifuge A WARNING This centrifuge weighs 121 kg 267 Ib Do not attempt to lift or move
2. 1 4 E 000 SHOW RPM RCE TIME TEMP C ao o D G aa m START ROGRA o 999 SAVE STOP O 5 9 DOOR Figure 1 2 Control Panel Digital Displays o SPEED TIME j TEMPERATURE 4 ROTOR Description The displays provide run information user messages and diagnostics During a run they show the actual real time operating conditions When the run parameters are being entered they show the set values selected Set values can be recalled during operation by pressing SHOW SET After 5 seconds the displays return to actual conditions Error messages see Section 4 also appear on the displays when applicable The centrifuge emits a series of audible tones to alert the user to an error condition Instructions for disabling the tones are in Section 3 Indicates rotor speed in revolutions per minute rpm or in relative centrifugal field a term describing the ratio of the centrifugal acceler ation at a specified radius and speed to the standard acceleration of gravity RCF x g natimed run indicates the remaining run time in hours and minutes The time display begins counting down when ENTER SAVE then START is pressed and continues counting down to 0 The run automatically ends when the set time reaches 0 and deceleration begins a tone sounds when the rotor stops spinning If a malfunction shuts down the centrifu
3. This centrifuge is not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials such as chloroform or ethyl alcohol in this centrifuge nor handle or store them within the 30 cm 1 ft area surrounding the centrifuge Mechanical Safety For safe operation of the equipment observe the following Use only the rotors and accessories designed for use in this centrifuge Do not exceed the maximum rated speed of the rotor in use NEVER attempt to slow or stop the rotor by hand Do not lift or move the centrifuge while the rotor is spinning NEVER attempt to override the door interlock system while the rotor is spinning Maintain a 7 6 cm 3 in clearance envelope around the centrifuge while it is running During operation you should come within the envelope only to adjust instrument controls if necessary Never bring any flammable substances within the 30 cm 1 ft area surrounding the centrifuge Never lean on the centrifuge or place items on the centrifuge while it is operating Chemical and Biological Safety Normal operation may involve the use of solutions and test samples that are pathogenic toxic or radioactive Such materials should not be used in this centrifuge however unless all necessary safety precautions are taken Observe all cautionary information printed on the original solution containers prior to their use Handle body fluids with care b
4. COULTER Allegra X 12 Series Centrifuges Instruction Manual Symbol Symbol Symbole S mbolo Simbolo z17 2 5035 Title Titel Tire Titulo Titolo HH Zu Dangerous voltage Gefahrliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo alta tensione EREE f E HEL As Attention consult accompanying documents Achtung Begleitpapiere beachten Attention consulter les documents joints Atenci n consulte los documentos adjuntos Attenzione consultare le informazioni allegate za AMARE BRO CS ER WEB air CE On power Ein Netzverbindung Marche mise sous tension Encendido Acceso sotto tensione A CE F RH Off power Aus Netzverbindung Arr t mise hors tension Apagado Spento fuori tensione U E K EM Protective earth grouna SchutzleiteranschluB Liaison a la terre Puesta a tierra de protecci n Collegamento di protezione a terra RETA Hel trap Bert Earth ground Erde Terre Tierra Scarica a terra PX END Bat A SAFETY NOTICE This safety notice summarizes information basic to the safe operation of the equipment described in this manual The international symbol displayed above is a reminder that all safety instructions should be read and understood before installation operation mainte nance or repair of this centrifuge When you see the symbol on other pages pay special att
5. ENTER SAVE ENTERING RUN SPEED Action 1 Press RPM RCF once to enter speed in RPM Press RPM RCF again to toggle between RPM and RCF modes 2 Use the keypad to enter required speed Operation To change an entry before you ve pressed ENTER SAVE or another parameter key enter a different value Press CE to abort changes and start over To change an entry after you ve pressed ENTER SAVE press the run parameter key again e Ifan unacceptable value is entered an out of range message is displayed in the message line Enter the correct value Result Enter a run speed up to the maximum speed of the rotor in use Or enter a relative centrifugal field RCF value up to the maximum achievable RCF of the rotor Result Allowed speed range for the selected rotor is displayed Entered speed appears on the speed display If the entered speed is out of the speed range of the installed rotor an error message will appear and the input will be cleared Enter an acceptable value from the range displayed Operation Action 3 Press the next run parameter key or ENTER SAVE ENTERING RUN TIME Action 1 Press TIME 2 Use the keypad or arrow keys to enter required time or Press TIME again for a hold run 3 Press the next run parameter key or ENTER SAVE Result You can change the set speed at any time during a run by repeating steps 1 through 3 The rotor will accelerate or decelera
6. Coulter office To help the field service represen tative diagnose and correct the problem gather as much informa tion about the situation as you can including the diagnostic number and message the operating situation when the diagnostic condition occurred such as rotor in use speed or load type and any unusual environmental and or operating conditions such as ambient temperature or voltage fluctuations Table 4 1 Diagnostic Message Chart Diagnostic Number Message Problem Result Recommended Action P1 Power failure occurred see manual System or facility power failure Run shuts down Press CE to clear message Restart run Remaining time shows on display L1 through L6 Door latch see Latches are not operating properly Error message appears run shuts Press down on both sides of the door until the DOOR LED comes on Press CE to manual down with maximum clear message If you close the door brake repeatedly and the problem continues call Beckman Coulter Field Service L7 Door latches do not Door doesn t open Press down on the door then press Can t open door see manual release when DOOR key is pressed DOOR again If door still doesn t open call Beckman Coulter Field Service Procedures for retrieving your sample are at the end of this section If the door opens but the message stays on the display press CE to clear C1 Rotor temp
7. It is your responsibility to decontaminate the centrifuge as well as any rotors and or accesso ries before requesting service by Beckman Coulter Field Service N WARNING Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and or mechanical injury Therefore turn the power off and disconnect the instrument from the main power source and refer such mainte nance to service personnel 5 1 Care and Maintenance MAINTENANCE CLEANING Rotor Cleaning Kit 339558 Perform the following procedures regularly to ensure continued performance and long service life of the centrifuge Inspect the centrifuge chamber for accumulations of sample dust or glass particles from broken sample tubes Clean as required see CLEANING below Check the air intake and exhaust for obstructions Keep vents clear and clean Wipe condensation out of the rotor chamber between runs with a sponge or clean cloth to prevent chamber icing If chamber icing occurs defrost the system and wipe moisture out of the chamber before use To defrost the system Allegra X 12R set the temperature to 30 C for 20 minutes These are suggested settings which may be adjusted as appropriate for your laboratory conditions I gt NOTE Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the propos
8. and follow all laws regarding the proper decontamination and safe disposal of electrical equipment For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection treatment recovery recycling and safe disposal of the device 1 Description CENTRIFUGE FUNCTION AND SAFETY FEATURES CENTRIFUGE FUNCTION The Beckman Coulter Allegra X 12R refrigerated or Allegra X 12 constant controlled temperature centrifuge see Figure 1 1 is a benchtop centrifuge that generates centrifugal forces required for a wide variety of applications Together with the Beckman Coulter rotors designed for use in this centrifuge the centrifuge applications include Routine processing such as sample preparations pelleting extrac tions purifications concentrations phase separations receptor binding and column centrifugations Cell isolation Binding studies and separation of whole blood Processing large numbers of small volume samples in multiwell plates for concentrating tissue culture cells cloning and replicate studies in vitro cytotoxicity studies receptor binding and genetic engineering experimentation Rapid sedimentation of protein precipitates large particles and cell debris The Allegra X 12 and X 12R are microprocessor controlled providing interactive operation The instrument de
9. exceeds 4C above set Rotor temperature exceeds tempera ture setting by more than 4 C but less than 8 C after equilibration Run continues the message is informational Press CE to clear message C2 Rotor temp exceeds 8C above set call Service Rotor temperature exceeds tempera ture setting by more than 8 C after equilibration Run shuts down with no brake Call Beckman Coulter Field Service C3 and C5 Temp error call Service Refrigeration system error Run shuts down with maximum brake Call Beckman Coulter Field Service Continued Troubleshooting Table 4 1 Diagnostic Message Chart continued Diagnostic Number Message Problem Result Recommended Action T1 and T2 Temp door locks for 45 minutes call Service System temperature problem Run shuts down with no brake door locks for 45 minutes Call Beckman Coulter Field Service T3 Temp door locks for 45 minutes see manual Motor overheated Run shuts down with no brake door locks for 45 minutes Wait 45 minutes for motor to cool down then restart run Press CE to clear message If problem persists call Beckman Coulter Field Service D1 through D9 Drive error see manual Drive system problem Run stops usually with no brake After run stops press CE to clear message then restart run If problem repeats call Beckman Coulter Field Service B
10. it without assistance SPACE AND LOCATION REQUIREMENTS N WARNING Do not place the centrifuge near areas containing flammable reagents or combustible fluids Vapors from these materials could enter the centrifuge air system and be ignited by the motor The centrifuge ships in a cardboard box on a wooden pallet For easy access remove the top of the box the foam insert on top of the centri fuge and then the upper part sides of the box and set them aside Then with the help of another person move the centrifuge from the pallet to its final position Position the centrifuge on a level surface such as a sturdy table or laboratory bench that is able to support the weight of the centrifuge 121 kg 267 Ib and resist vibration Make sure that the centrifuge front feet are fully supported on the table 2 1 Installation 2 2 Locate the centrifuge in an area with sufficient ventilation to allow for heat dissipation e Check that there are 7 6 cm 3 in clearances at the sides and back of the centrifuge to ensure sufficient air circulation Dimensions are shown in Figure 2 1 Additional clearance is required on the left side to allow access to the power switch The centrifuge must have adequate air ventilation to ensure compliance to local requirements for vapors produced during operation Ambient temperatures during operation should not be lower than 10 C 50 F or higher than 35 C 95 C Relative humidity should no
11. maintain the selected rotor temperature Run temperature in the Allegra X 12R can be set between 10 and 40 C Temperature in the Allegra X 12 is factory set at 20 C and is maintained at between 18 and 22 C to prevent sample heating gt NOTE To avoid chamber icing refrigeration is off when the door is open The centrifuge door must be closed and locked for the refrigeration system to begin operating 1 3 Description DRIVE The asynchronous direct drive motor is brushless for clean quiet operation The resilient suspension ensures that loads will not be disturbed by vibration and prevents damage to the drive shaft if an imbalance occurs during centrifugation Maximum acceleration and deceleration may be selected to allow fast processing of samples alternately delicate gradients may be preserved using slower acceler ation and deceleration CONTROLS AND INDICATORS POWER SWITCH CONTROL PANEL The power switch located on the left side of the centrifuge controls electrical power to the centrifuge It is also a circuit breaker that will trip to cut off power in the event of a power overload The power switch must be turned on before the chamber door can be opened The control panel see Figure 1 2 is mounted at an angle on the centrifuge front for easy visibility and access It is used to enter run parameters via function keys and a keypad and to display run param eters program information and user messages
12. on 2 Press DOOR The centrifuge will accept this command only when the rotor is at rest 3 Install the rotor according to directions in the rotor manual Ensure that the rotor Is seated on the drive hub Avoid bumping the control panel keys during rotor installation or removal 4 Close the chamber door by pressing down firmly on both sides MANUAL OPERATION Result The control panel display shows Allegra X 12R or Allegra X 12 and the software version The door opens The door locks When the latches engage the LED next to the DOOR key lights up I gt NOTE When using multiwell plate carriers enter SX4750A as the rotor and limit top speed of the instrument to 3750 rpm in an Allegra X 12 series centrifuge or 4450 rpm in an Allegra X 15R when running up to three stacked Beckman Coulter plates separated by cap strips reduce speed to 2700 rpm if running four stacked plates Set rotor speed using rpm values only To set speed to a specific RCF use the rpm to RCF conversion chart GX TB 015 to determine the applicable rpm to enter e When a run parameter key ROTOR RPM RCF TIME TEMP ACCEL or DECEL is pressed a parameter can be entered or changed Entry is completed when ENTER SAVE or another func tion key is pressed SELECTING A ROTOR Action 1 Press ROTOR then use the arrow keys or press ROTOR again to select a rotor 2 Press the next run parameter key or press
13. 7 4 24 3 16 3 46 3 14 6 59 7 02 3 3 49 3 53 4 34 3 52 4 37 3 53 8 33 7 54 2 4 43 4 49 4 59 4 47 6 11 4 49 8 49 8 50 1 SLOW 6 15 6 21 6 54 6 24 6 15 6 20 10 11 10 18 0 OFF coast coast coast coast Action Result 3 Press DECEL then use the Entered deceleration rate appears on the display keypad or arrow keys to select the required decelera tion rate 4 Press the next run parameter key or ENTER SAVE STARTING A RUN Action Result 1 Press ENTER SAVE and The light next to the START key flashes and the rotor begins to spin START 3 8 Operation Action Result I gt NOTE To begin a run ENTER SAVE must always be the last key pressed before pressing START If you wait more than 5 seconds the START key will not activate If this happens press ENTER SAVE and START again to begin the run The run will end when the time display counts down to zero Press STOP to terminate a run for any reason The green light next to the STOP key will flash while the rotor decelerates A tone sounds when the rotor comes to a complete stop Instructions for disabling the tone are at the end of this section 2 After the rotor has stopped The door opens and the LED lights press DOOR A CAUTION 3 Remove the rotor or If disassembly reveals evidence of leakage you containers should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge
14. ade screwdriver to pry the cap loose RETRIEVING YOUR SAMPLE IN CASE OF POWER FAILURE If facility power fails you will have to restart the run when the power is restored In the event of an extended power failure it may be neces sary to trip the door locking mechanism manually to remove the rotor and retrieve your sample Result No displays or LEDs are lit No sound or vibration comes from the centrifuge A WARNING Never attempt to override the door interlock system while the rotor is spinning b Release device cap Figure 4 1 Door Latch Override Action 5 Insert the screwdriver through the two cords and pull to release 6 Reinstall the cap in the opening after sample retrieval Troubleshooting Result The latch releases and the door opens If the rotor is still spinning close the door and wait until it stops before attempting to remove it A WARNING Never attempt to slow or stop the rotor by hand INSTRUMENT CARE 5 Care and Maintenance This section contains care and maintenance procedures that should be performed regularly For maintenance not covered in this manual contact Beckman Coulter Field Service 1 800 742 2345 in the United States worldwide offices are listed on the inside back cover of this manual for assistance User messages and recommended actions are discussed in Section 4 TROUBLESHOOTING INN NOTE
15. allowable speed of the rotor An imbalance detector monitors the rotor during the run causing automatic shutdown if rotor loads are severely out of balance At low speeds an incorrectly loaded rotor can cause imbalance Rotor instability can also occur if the centrifuge is moved while running or if it is not resting on a level and secure surface see Section 4 TROUBLESHOOTING Description CENTRIFUGE CHASSIS HOUSING The centrifuge housing is made of sheet steel and structural foam and is finished with acrylic baking enamel The control panel overlay is made of coated polystyrene copolymer DOOR The aluminum and structural foam door is secured to the housing by solid hinges A window in the center allows strobe viewing When the door is closed the locking system engages The door is locked when a run is in progress and can be opened only when the rotor is stopped and the LED next to the DOOR key is lit If there is a power failure the door lock can be manually tripped for sample recovery see Section 4 TROUBLESHOOTING ROTOR CHAMBER The aluminum rotor chamber is coated with epoxy enamel for corro sion resistance The chamber is sealed by a foam gasket TEMPERATURE SENSING AND CONTROL With the power on the temperature control system is activated when the door is closed and locked A sensor in the rotor chamber continu ously monitors chamber temperature The microprocessor calculates the required chamber temperature to
16. ance to the Buyer in obtaining from the respec tive manufacturer whatever adjustment is reasonable in light of the manufacturer s own warranty Beckman Coulter shall be released from all obligations under all warranties either expressed or implied if the product s covered hereby are repaired or modified by persons other than its own authorized service personnel unless such repair in the sole opinion of Beckman Coulter is minor or unless such modification is merely the installation of a new Beckman Coulter plug in component for such product s DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WAR RANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANT ABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CON SEQUENTIAL DAMAGES OF ANY KIND WHATSO EVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT B BECKMAN COULTER Beckman Coulter Inc 250 S Kraemer Blvd Brea California 92821 Sales and Service 1 800 742 2345 Internet www beckmancoulter com 2009 Beckman Coulter Inc All rights reserved
17. and Fuses 0 0 KK KK KK KK KK KK IK nee 5 4 Storage and Transport W K kk KK KK KK KK KK KK KK KK KK e 5 4 SLOFAge ouo DS A Coste e ate ROS cte P Re 5 4 Supply Listas daria A O an 5 5 Replacement Par s 42 coeden a kw ake k Zala akla ka 5 5 Nn c ME Cr 5 5 Contents Illustrations Page Figure 1 1 The Centritu ges otek 4 orc ERE Ge sms 1 2 Figure 1 2 Control Panel airis rea a EL on datas dake 1 4 Figure 2 1 Centrifuge Dimensions ooooccocoocccoconc KK KK KK eee eee 2 2 Figure 4 1 Door Latch Override 0 KK KK KK KK KK KK KK KK KK KK KK KK 4 6 Tables Page Table 3 1 Acceleration and Deceleration Rates in Minutes Seconds to and from Maximum Speed 2 2222 0 0 RR KK KK KK KK 3 8 Table 4 1 Diagnostic Message Chart LA AA KK KK KK KK KK KK KK KK KK KK KK 4 2 Table 4 2 Troubleshooting Chart WA kK KK KK KK KK KK KK KK KK KK KK KK KH 4 5 vii CERTIFICATION SCOPE OF MANUAL Introduction To ensure full system quality Beckman Coulter Allegra X 12 series centrifuges are manufactured in a registered ISO 9001 or 13485 facility They have been designed and tested to be compliant when used with Beckman Coulter rotors with the laboratory equipment requirements of applicable regulatory agencies Declarations of conformity and certificates of compliance are available at www beckmancoulter com This manual is designed to familiarize you with the Beckman Coulter Allegra X 12
18. and accessories gt NOTE Depending upon the duration of the event transient power line interruptions may cause this equipment to decelerate or reset with the possible loss of in process operation If you suspect this has occurred simply repeat the runs PULSE FUNCTION The pulse function accessed by pressing the PULSE key is used for short duration runs as follows 3 9 Operation e When the PULSE key is pressed the rotor accelerates at maximum rate to the set speed and continues to spin as long as the PULSE key is pressed The current run time acceleration and decelera tion settings are overridden by the pulse function When the PULSE key is released the rotor begins decelerating to 0 rpm using maximum deceleration Using the pulse function eliminates the need to press the START and STOP keys When the PULSE key is pressed the TIME display begins displaying the elapsed seconds When the PULSE key is released the seconds stop accumulating e The centrifuge memory retains the parameters of the last run performed before the PULSE key was pressed At the end of a pulse run after the centrifuge door is opened and closed the previous run parameters are displayed PROGRAMMED OPERATION The instrument internal memory can store up to 10 programs which can be recalled by keypad numbers 0 through 9 Saved programs are retained in memory even if the power is turned off Procedures for entering run parame
19. aphic Conventions 000 ccc KEREKER KK xi CFC Free Centrifugation csse eene xi Radio Interference ccce xii Canadian Regulations esses xii Recycling Lapel gt lt Xw ninin aw nn Rd ERI NIS xii SECTION 1 DESCRIPTION Centrifuge Function and Safety Features 22222220220 1 1 Centrifuge Function seuss l se alay l w e dl Ba a0 ee 1 1 Safety Features sese iaa pia uA ra FE e SEA 1 2 Centtituge Cliassis nur EPIS vex kak heke X n eee 1 3 HOUSING z P ne Ki een da SR ENS ak ye re 1 3 bro ME E 1 3 Rotor Chamber eines erede bai de 1 3 Temperature Sensing and Control 00005 1 3 Dive A is A a da td 1 4 111 Contents iv SECTION 2 SECTION 3 Page Controls and Indicators 0 0 0 eee ee eens 1 4 Power Switch lesse 1 4 Control Panel osc eae say Ged n Kiye ee do eave Ged Gee aad S 1 4 Name Rating Platens resini Ter E E eee eee 1 8 Specifications ur peers a 1 9 Ayailable ROtor A sw A are 1 10 INSTALLATION Space and Location Requirements 00 0002 eee eee 2 1 Electrical Requirements KK KK KK KK KK KK KK KK KK K eee 2 3 Securing the Centrifuge to the Bench 008 2 3 Test Run 5 sa2 w mna k E m nad whale SENA Vra Bes rius 2 4 OPERATION Summary of Run Procedures KK KK KK KK KK KK KK KK 3 2 Manual Run is W UA SIY ee Mn W W lan S Weed 3 2 Programmed Run u
20. as pressed The run starts the green light next to the START key flashes and the rotor begins to spin I gt NOTE To begin a run ENTER SAVE must always be the last key pressed before pressing START If you wait more than 5 seconds the START key will not activate If this happens press ENTER SAVE and START again to begin the run The run will end automatically when the time display counts down to Zero Press STOP to terminate a run at any time The green light next to the STOP key will flash while the rotor decelerates A tone will sound when the rotor has come to a complete stop Operation Action Result 3 After the rotor has stopped The door opens and the LED is lit press DOOR 4 Remove the rotor or containers DISABLING ENABLING THE AUDIBLE TONES The audible tones beeps that sound when a run parameter key is pressed or at the end of each run or if an error occurs may be turned on and off by following the steps below rotor must be at 0 rpm 1 To disable the key or error tone press ENTER then press 2 3 6 The words Disable KEY beep appear on the display To disable the end of run tone press ENTER then press 2 2 3 The words Disable RUN beep appear on the display Tones that have been disabled can be enabled as follows 1 To enable a key or error tone press ENTER then press 2 3 5 The words Enable KEY beep appear on the d
21. ay Pressing CE will also clear some diagnostic messages If a diagnostic message does not clear when CE is pressed see Section 4 TROUBLESHOOTING 1 7 Description 1 8 SHOW PULSE START STOP NAME RATING PLATE Pressed during a run to display set values for 5 seconds Causes the rotor to accelerate at maximum rate to set speed for short duration runs as long as the key is pressed Deceleration at maximum rate begins when the key is released Pressing ENTER SAVE then START begins the run START must be pressed within 5 seconds of pressing ENTER SAVE This key combi nation can also be used to abort a deceleration process and restart the centrifuge Can be pressed at any time while the rotor is spinning to terminate a run The centrifuge decelerates to a complete stop according to the deceleration setting The green light next to the key flashes while the rotor decelerates A tone sounds when the rotor comes to a complete stop Instructions for disabling the tone are in Section 3 Unlocks and opens the door The instrument will not accept the unlock command if the rotor is spinning A name rating plate is affixed to the rear of the instrument Always mention the serial number and model number when contacting Beckman Coulter regarding your centrifuge Description SPECIFICATIONS Only values with tolerances or limits are guaranteed data Values without tolerances are informative data withou
22. d eleven deceleration rates to protect the gradient and sample to gradient interface Select rates depending on the type of run you are performing for pelleting runs where sample mixing is not a concern maximum acceleration and deceleration can be used If running delicate gradients a lower setting may be needed Typical acceleration and deceleration rates are listed in Table 3 1 If no rate is selected the centrifuge automatically accelerates and decelerates at maximum Action Result 1 Press ACCEL then use the Entered acceleration rate appears on the display keypad or arrow keys to select the required accelera tion rate 2 Press the next run parameter key or ENTER SAVE 3 7 Operation Table 3 1 Acceleration and Deceleration Rates in Minutes Seconds to and from Maximum Speed Times shown are with the rotor fully loaded in the Allegra X 12R Times are approximate actual times will vary depending on the rotor in use the rotor load run speed and voltage fluctuations SX4750 SX4750u SX4750A FX6100 3 750 RPM 3 750 RPM 3 750 RPM 10 200 RPM Rate Accel Decel Accel Decel Accel Decel Accel Decel 10 MAX 1 21 1 15 1 16 1 09 1 19 1 15 1 33 1 23 9 1 51 1 55 1 51 1 52 1 51 1 52 2 30 2 22 8 2 00 2 06 2 02 2 04 2 01 2 03 3 36 3 14 7 2 12 2 17 2 23 2 13 2 13 2 17 4 52 4 09 6 2 28 2 29 3 04 2 26 2 55 2 28 5 12 5 08 5 2 47 2 49 3 18 2 47 3 11 2 48 6 05 6 03 4 3 12 3 1
23. e displayed on the centrifuge and are reproduced and described below and on the inside of the front cover NOTES CAUTIONS AND WARNINGS gt NOTE Used to call attention to important information that should be followed during installation use or servicing of this equipment A CAUTION Used to indicate a potentially hazardous situa tion which ifnot avoided may result in minor or moderate injury and or mechanical damage It is also used to alert against unsafe practices Introduction A WARNING Used whenever an action or condition may potentially cause personal injury or loss of life Mechanical damage may also result AN WARNING Indicates high voltage or risk of electric shock Refer servicing of all areas displaying this symbol to service personnel TYPOGRAPHIC CONVENTIONS Certain typographic conventions are used throughout this manual to distinguish names of user interface components such as keys and displays Key names for example START or ROTOR appear in capital letters within brackets CFC FREE CENTRIFUGATION To ensure minimal environmental impact no CFCs are used in the manufacture or operation of Allegra X 12 series centrifuges xi Introduction xii RADIO INTERFERENCE CANADIAN REGULATIONS RECYCLING LABEL AZ A28219 AA Note On the instrument the triangle background is yellow rather than gray This equipment has been tested and found to comply with the limit
24. e centrifuge on the bench or table Press CE to clear message Centrifuge was moved during operation Run shuts down with maximum brake After the rotor comes to a complete stop open and close the centrifuge door then restart Press CE to clear message Drive error mechani cal damage Run shuts down with maximum brake Call Beckman Coulter Field Service 12 Imbalance error see manual System imbalance error Run shuts down with maximum brake Press CE to clear message then restart run If problem persists call Beckman Coulter Field Service Troubleshooting OTHER POSSIBLE PROBLEMS Operating problems that may not be indicated by diagnostic messages are described in Table 4 2 along with probable causes listed in the probable order of occurrence and corrective actions Perform the recommended corrective action in sequence as listed If you are unable to correct the problem call Beckman Coulter Field Service Table 4 2 Troubleshooting Chart Problem Problem Result Recommended Action Rotor cannot Line voltage below rating Have a qualified service person measure line voltage achieve set while the instrument is operating speed Electrical failure Make sure the power cord is securely connected call Beckman Coulter Field Service Motor failure Call Beckman Coulter Field Service Door will not Rotor spinning Wait until the rotor s
25. ecause they can transmit disease No known test offers complete assurance that they are free of micro organisms Some of the most virulent Hepatitis B and C and HIV I V viruses atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this centrifuge without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection Dispose of all waste solutions according to appropriate environmental health and safety guidelines It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter A GX IM 5 m January 2009 anuar Allegra X 12 Series Centrifuges Instruction Manual 2009 Beckman Coulter Inc Contents Page INTRODUCTION Certification x oiu 22 e ese de ee ure JAXm_MMM ENRP DO ix Scope of Manual csse hh ix CONVENTIONS os Le e eee eR tete tete ete x Notes Cautions and Warnings cssc x Typogr
26. eceptacle strip e Tf there is any question about voltage have a qualified service person measure it under load while the drive is operating To ensure safety the centrifuge should be wired to a remote emer gency switch preferably outside the room where the centrifuge is housed or adjacent to the exit from that room in order to disconnect the centrifuge from the main power source in case of a malfunction SECURING THE CENTRIFUGE TO THE BENCH Allegra X 12 series centrifuges are certified to meet the requirements of the European CE mark To meet these requirements the centrifuge must be secured to the bench using the anchoring kit shipped with the instrument This will prevent the centrifuge from moving in the unlikely event of a rotor mishap Complete instructions for installing the anchoring kit are packaged with the hardware which is shipped with the centrifuge The instruc tions publication GX TB 002 include a full size template to be used as a guide for drilling holes in the bench Refer to this document for installation instructions Installation 2 4 TEST RUN I gt NOTE The centrifuge must be plugged in and the power switch turned to on position before the door can be opened We recommend that you make a test run to ensure that the centrifuge is in proper operating condition following shipment See Section 3 for instructions on operating the centrifuge After completing the test run return the pre addres
27. ed method will not damage the equipment Clean the centrifuge frequently Always clean up spills when they Occur to prevent corrosives or contaminants from drying on compo nent surfaces To prevent accumulations of sample dust and or glass particles from broken sample tubes keep the chamber clean and dry by frequent wiping with a cloth or paper towel For thorough cleaning wash the chamber using a mild detergent such as Beckman Solution 555 diluted 10 to 1 with water Rinse thoroughly and dry completely Tube Breakage DECONTAMINATION Care and Maintenance Clean the centrifuge exterior surfaces by wiping with a cloth dampened with Solution 555 diluted 10 to 1 with water Do not use acetone Remove the rotor from the centrifuge and clean the drive hub regularly using Solution 555 diluted 10 to 1 with water and a soft brush Rinse thoroughly and dry completely If a glass tube breaks and all the glass is not contained in the bucket or rotor be sure to thoroughly clean the chamber N WARNING Be careful when examining or cleaning the chamber and chamber gasket as sharp glass fragments may be embedded in their surfaces Examine the chamber gasket to make sure that no glass particles are retained in it Carefully remove any glass particles that may remain Carefully wipe away any glass particles that remain in the chamber If the centrifuge and or accessories are contaminated with radioactive or path
28. efore trying to open the door listen carefully and make sure that no sound is coming from the chamber indicating a spinning rotor Follow the directions under RETRIEVING YOUR SAMPLE IN CASE OF POWER FAILURE below Ri and R2 Rotor ID problem see manual No magnets identified or magnets incorrectly identified Run shuts down with maximum brake Check that magnets are in place on rotor bottom Press CE to clear message If problem repeats call Beckman Coulter Field Service R3 Rotor ID mismatch check rotor The entered rotor number is not the same as the rotor identified Run shuts down with maximum brake Press CE to clear message Enter the correct rotor entry identification R4 and R5 No rotor match see manual The system cannot identify the rotor Run shuts down with maximum brake Make sure the rotor in use is a compatible Beckman Coulter rotor see AVAILABLE ROTORS in Section 1 Check that magnets are in place on rotor bottom Press CE to clear the message Call Beckman Coulter Field Service S1 System error call Service There is a problem with the system control soft ware EPROM or RAM System shuts down no brake Call Beckman Coulter Field Service S8 System error door locks for 45 minutes see manual There is a problem with system control software Run shuts down with no brake door locks for 45 minutes Wait 45 minu
29. ention to the safety information presented Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the centrifuge Safety During Installation and or Maintenance This centrifuge weighs 121 kg 267 Ib Do not attempt to lift or move it without assistance Any servicing of this equipment that requires removal of any covers can expose parts that involve the risk of electric shock or personal injury Make sure that the power switch is off and the centrifuge is disconnected from the main power source and refer such servicing to qualified personnel Do not replace any centrifuge components with parts not specified for use on this instrument Electrical Safety To reduce the risk of electrical shock this equipment uses a three wire electrical cord and plug to connect the centrifuge to earth ground To preserve this safety feature Make sure that the matching wall outlet receptacle is properly wired and earth grounded Check that the line voltage agrees with the voltage listed on the name rating plate affixed to the centrifuge Never use a three to two wire plug adapter Never use a two wire extension cord or a two wire non grounding type of multiple outlet receptacle strip Do not place containers holding liquid on or near the chamber door If they spill liquid may get into the centrifuge and damage electrical or mechanical components Safety Against Risk of Fire
30. ge during a run when the instrument is turned back on the time will not remain and 00 00 will be displayed in the time window By comparing this time with the original set time you can determine when the run ended nahold run indicates the time elapsed since START was pressed After 99 hours and 59 minutes the timer resets to 0 and continues counting elapsed time na pulse momentary run indicates the time in minutes and seconds elapsed since PULSE was pressed Time continues to count up until the PULSE key is released The total elapsed time is then displayed until another key is pressed Indicates rotor temperature in degrees C at thermoequilibrium Displays the name of the selected rotor 1 5 Description ACCEL DECEL O PROGRAM Run Parameter Keys Cc ROTOR Q RPM RCF When the ACCEL key is pressed one of ten available acceleration rates can be entered Displays the selected acceleration rate through out the run When the DECEL key is pressed one of eleven available deceleration rates can be entered Displays the selected deceleration rate through out the run During setup when the PROGRAM key is pressed a program number one of ten possible can be selected and program parameters entered During centrifugation the number of the program being run is displayed Run parameter keys are used to enter specific run information as follows The centrifuge emits an audible to
31. isplay To enable the end of run tone press ENTER then press 2 2 2 The words Enable RUN beep appear on the display SN USER MESSAGES 4 Troubleshooting This section lists possible malfunctions together with probable causes and corrective actions Maintenance procedures are given in Section 5 For any problems not covered here contact Beckman Coulter Field Service 1 800 742 2345 in the United States worldwide offices are listed on the inside back cover of this manual for assistance IlIi NOTE It is your responsibility to decontaminate the instrument as well as any rotors and or accesso ries before requesting service by Beckman Coulter Field Service User messages appear on the display to communicate information about the instrument or to alert you to abnormal conditions that need attention Help and informational messages caused by incorrect input or certain operating conditions can be cleared by pressing CE and following the instructions in the message Diagnostic messages that result from abnormal operating condi tions or equipment malfunction require troubleshooting Refer to Table 4 1 to determine the nature of the condition and any recom mended actions If a problem persists after you have performed the recommended action call Beckman Coulter Field Service in the United States call 1 800 742 2345 outside the U S call your 4 1 Troubleshooting 4 2 local Beckman
32. kk kK KK KK KK KK KK KK KK KK KK KK KK 3 3 Preparation un fea Hes Gee oe pasar en Kn a Ses 3 3 Installing the Rotor KK KK KK KK KK KK KK KK KK KK K KK 3 3 Manual Operation llle 3 4 Selecting ROt r 3A b Xala yk ula en Kula yn Zall 3 5 Entering Run Speed ae na ein nn 3 5 Entering Run Time Asan b ks eee l k e eee 3 6 Entering Run Temperature Allegra X 12R Only 3 6 Entering Acceleration and Deceleration Rates 3 7 Starting a Run 2 Va p Lp e er REA 3 8 Pulse Function uerb reenUxEMDIH UNE E wating hom fee 3 9 SECTION 4 SECTION 5 Warranty Page Programmed Operation AK KK KK KK KK KK KK KK KK KK KK K 3 10 Creating a New Program 2 0 KK KK KK KK KK KK KK 3 10 Recalling a Program 0 0 kK KK KK KK KK KK KK KK KK KK 3 11 Disabling Enabling the Audible Tones 3 12 TROUBLESHOOTING User Messages a sm 2 t Ree here er nn hn e pa Karen 4 1 Other Possible Problems u KK KK KK KK KK KK KK KK KK 4 5 Retrieving Your Sample in Case of Power Failure 4 6 CARE AND MAINTENANCE Instrument Care 4 2 lk kulk Wl n WE W WAW W k W 5 1 Maintenances aussen ebbe n oe obese Jhd 5 2 Cleaning 5s ae b ient UE eitis 5 2 Decontamination 0 0 eee 914 315a e 5 3 Sterilization and Disinfection 0 0 00 KK KK 5 3 Circuit Breaker
33. n Allegra X 12 Series centrifuge the product to the original buyer by Beckman Coulter or by an authorized representative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use Some components and accessories by their nature are not intended to and will not function for as long as one 1 year A complete list of such components or accessories is maintained at the factory and at each Beckman Coulter District Sales Office The lists applicable to the products sold hereunder shall be deemed to be part of this warranty If any such compo nent or accessory fails to give reasonable service for a reason able period of time Beckman Coulter will repair or at its election replace such component or accessory What consti tutes either reasonable service and a reasonable period of time shall be determined solely by Beckman Coulter REPLACEMENT Any product claimed to be defective must if requested by Beckman Coulter be returned to the factory transportation charges prepaid and will be returned to Buyer with the trans portation charges collect unless the product is found to be defective in which case Beckman Coulter will pay all trans portation charges CONDITIONS Beckman Coulter makes no warranty concerning products or accessories not manufactured by it In the event of failure of any such product or accessory Beckman Coulter will give rea sonable assist
34. ne when each key is pressed Instructions for disabling the tone are in Section 3 Pressed to display scrollable list of compatible rotors Can be pressed repeatedly to scroll through the rotor list or the arrow keys can be used for scrolling If the display shows speed in rpm the key is pressed once to enter run speed using the keypad in rpm revolutions per minute pressed twice to enter run speed in RCF relative centrifugal field If the display shows speed in RCF the key is pressed once to enter speed in RCF or twice to enter speed in rpm Pressed to enter run time using the keypad When TIME is pressed twice HOLD mode is selected Pressing it again reverts to time entry mode Timed run Run time up to 99 hours and 59 minutes can be set If more than 59 minutes are entered in the minutes field the system automatically converts the entry to hours and minutes Decelera tion begins when the set time counts down to 0 Continuous run hold For runs of unspecified lengths hold mode is used When 99 hours 59 minutes is reached the system TEMP C 3 En DECEL PROGRAM ENTER 8 oo y E eX OQ CCS 60060 Description resets to O and continues counting A hold run continues until the STOP key is pressed Allegra X 12R only Pressed to enter run temperature using the keypad from 10 to 40 C If a new temperature is not entered the centrifuge uses the temperature set for the previous
35. ogenic solutions perform all appropriate safety and decontam ination procedures Refer to Chemical Resistances publication IN 175 to be sure the decontamination method will not damage any part of the instrument 5 3 Care and Maintenance STERILIZATION AND DISINFECTION The centrifuge is finished with acrylic baking enamel Ethanol 70 may be used on this surface See Chemical Resistances for chemical compatibilities of centrifuge and accessory materials While Beckman Coulter has tested ethanol 70 and found that it does not damage the centrifuge no guarantee of sterility or disinfec tion is expressed or implied When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods to use CIRCUIT BREAKER AND FUSES There are no user replaceable fuses in the centrifuge If the centrifuge circuit breaker trips for any reason the power switch will move to the off O position Reset the circuit breaker by turning the power switch back to the on I position If it trips again immedi ately do not reset it Call Beckman Coulter Field Service A CAUTION Repeated attempts to reset the centrifuge circuit breaker can cause substantial damage to electrical and electronic components STORAGE AND TRANSPORT STORAGE To ensure that the centrifuge does not get damaged contact Beckman Coulter Field Service for specific instructions and or assistance in preparing the equipment for t
36. opolymer Housing surfaces 0 000000 acrylic baking enamel Electrical requirements 208 V 60 Hz instrument 187 229 VAC 9 A 60 Hz 200 V 50 60 Hz instrument 180 220 VAC 10 A 50 60 Hz 230 V 50 Hz instrument 207 253 VAC 8 A 50 Hz Electrical supply seb bie b ye he tadashi een Class I Maximum heat dissipation into room under steady state conditions 0e eee 4100 Btu hr 1 2 kW Noise level 0 91 m 3 ft in front of centrifuge lt 68 dBa Installation overvoltage category 0 WW KK KK KK KK KK KK KK eee II Pollution degree ML AS KK KK KK KK KK KK KK KK KK KK KK KK KK KK 21 During transient conditions such as acceleration and deceleration rotor temperature may be outside this range To reach temperatures above ambient the centrifuge is dependent on the frictional heat generated inside the chamber during operation At low run speeds or low ambient temperatures the centrifuge may not be able to achieve some higher temperatures Normally only nonconductive pollution occurs occasionally however a temporary conductivity caused by condensation must be expected 1 9 Description AVAILABLE ROTORS The following Beckman Coulter rotors can be used in the Allegra X 12 series centrifuge The rotors are described in individual manuals that accompany each rotor
37. ot use the centrifuge in the vicinity of flammable liquids or vapors and do not run such materials in the instrument Do not lean on the centrifuge or place items on it while it is operating 3 1 Operation SUMMARY OF RUN PROCEDURES For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration MANUAL RUN 1 POWER Turn the power switch on 2 DOOR Press DOOR to open the chamber door lift the door open 3 Install the rotor according to the applicable rotor manual then close the chamber door 4 ROTOR Press ROTOR then press ROTOR again or use the arrow keys to select the rotor 5 RPM RCF Press RPM RCF then use the keypad to enter the run speed Press RPM RCF a second time to toggle between RPM and RCF modes 6 TIME Press TIME then use the keypad to enter the run time to 99 hrs 59 minutes or press TIME twice to toggle between hold continuous run and set time mode 7 TEMP C Press TEMP C then use the keypad to enter the required run temperature 10 to 40 C Allegra X 12R only 8 ACCEL Press ACCEL then use the keypad or arrow keys to enter the selected acceleration rate number 1 SLOW through 10 MAX 9 DECEL Press DECEL then use the keypad or arrow keys to enter the selected deceleration rate number 0 OFF through 10 MAX 10 ENTER SAVE Check that all parameters are correct and tha
38. ransport or long term storage Temperature and humidity conditions for storage should meet the environmental requirements described under SPECIFICATIONS in Section 1 Flammability hazard Do not use in or near operating centrifuges SUPPLY LIST REPLACEMENT PARTS SUPPLIES Care and Maintenance Call Beckman Coulter Sales 1 800 742 2345 in the United States worldwide offices are listed on the back cover of this manual for detailed information on ordering parts and supplies For your convenience a partial list is given below I gt NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at 1 800 742 2345 in the United States or by contacting your local Beckman Coulter office Anti totatlon Kit s gt eds ose 2a4 Seed DES hee oS ee oe Dua 392482 Spinkote lubricant 2 OZ KK cee KK KK KK KK KK KK KK KK KK 306812 Silicone vacuum grease 1 OZ WW KK KK KK KK KK KK KK KK 335148 Beckman Solution 555 1 qt KK KK KK KK KK KK KK KK 339555 Program library record 0 0 0 0 KK KK KK KK KK KK KK KK KK 233679 ALLEGRA X 12 SERIES CENTRIFUGE WARRANTY Subject to the exceptions and upon the conditions specified below and the warranty clause of the Beckman Coulter Inc terms and conditions in effect at the time of sale Beckman Coulter agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within one 1 year after delivery of a
39. run Enables selection of one of ten preset acceleration rates see Table 3 1 in Section 3 available to protect specific gradient and sample to gradient interfaces If no acceleration rate is selected the centrifuge defaults to maximum acceleration Enables selection of one of eleven preset deceleration rates see Table 3 1 in Section 3 available to maintain optimum separation If no deceleration rate is selected the centrifuge defaults to maximum deceleration Deceleration rate 0 is always coast to complete stop A toggle switch used to enter into and exit out of the program mode to create or recall programs When the key is pressed the program number can be selected using the keypad or arrow keys Up and down arrow keys which can be pressed to increment parameter values up or down The ENTER SAVE key can be pressed to save parameter inputs to system memory during manual run setup or to a program during a program set up and to start the centrifuge ENTER SAVE then START must be pressed to start the centrifuge The keypad is used to enter numerical run parameters to enter or recall a program number and to select acceleration and or decelera tion rates In addition to keys 0 through 9 the keypad includes a plus minus key and a CE clear entry key The key is used to enter a temperature setting below 0 C for example 2 C in the Allegra X 12R The CE clear entry key clears any parameter on the displ
40. s for a Class A digital device pursuant to Part 15 of FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environ ment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with this instruction manual may cause interference to radio communica tions Operation of this equipment in a residential area is likely to cause interference in which case the user will be required to correct the interference at his own expense This equipment does not exceed the Class A limits for radio noise emissions from digital apparatus as set out in the radio interference regulations of the Canadian Department of Communications Le pr sent appareil num rique n met pas de bruits radio lectriques d passant les limites applicables aux appareils num riques de Classe A prescrites dans le reglement sur le brouillage radioelectrique dict par le Minist re des Communications du Canada This symbol is required in accordance with the Waste Electrical and Electronic Equipment WEEE Directive of the European Union The presence of this marking on the product indicates 1 the device was put on the European market after August 13 2005 and 2 the device is not to be disposed via the municipal waste collection system of any member state of the European Union It is very important that customers understand
41. sed warranty card included with this literature This will validate the centrifuge warranty and ensure your receipt of further information regarding new accessories and or modifications as they become available 3 Operation This section contains centrifuge operating procedures A summary is provided at the start of this section If you are an experienced user of this centrifuge you can turn to the summary for a quick review of operating steps Refer to the applicable rotor manual for instructions on preparing the rotor for centrifugation N WARNING Handle body fluids with care because they can transmit disease No known test offers complete assurance that they are free of micro organisms Some of the most virulent Hepatitis B and C and HIV I V viruses atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Handle other infec tious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this centrifuge with out taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection Do n
42. series centrifuges their functions specifications opera tion and routine operator care and maintenance We recommend that you read this entire manual especially the SAFETY NOTICE and all safety related information before operating the centrifuge or performing instrument maintenance Section 1 contains system specifications and a brief physical and functional description of the centrifuge including the operating controls and indicators Section 2 provides requirements for preparing laboratory facilities and installing the centrifuge Section 3 contains centrifuge operating procedures Section 4 lists diagnostic messages and other possible malfunc tions together with probable causes and suggested corrective actions Introduction Section 5 contains procedures for routine operator care and main tenance as well as a brief list of supplies and replacement parts I gt NOTE If the centrifuge is used in a manner other than specified in this manual the safety and perfor mance of this equipment could be impaired Further the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety Use of any equipment not specifically recommended in this manual and or the applicable rotor manual is the sole responsibility of the user CONVENTIONS Certain symbols are used in this manual to call out safety related and other important information These international symbols may also b
43. sign features a brushless asynchronous motor automatic rotor identification system program memory that enables repeated run conditions temperature control system and a choice of acceleration and deceleration rates User messages alert the operator to conditions that may need attention 1 1 Description SAFETY FEATURES Figure 1 1 The Centrifuge Allegra X 12 series centrifuges have been designed and tested to operate safely indoors at altitudes up to 2 000 m 6 562 ft Safety features include the following An electromechanical door lock system prevents operator contact with spinning rotors and prevents run initiation unless the door is shut and locked The door is locked when a run is in progress and can be opened only when the rotor is stopped and the LED light emitting diode next to the DOOR key is lit If there is a power failure the door lock can be manually tripped for sample recovery A steel barrier surrounds the rotor chamber to provide full operator protection A rotor identification system prevents the installed rotor from running above its maximum rated speed During acceleration the microprocessor checks the magnetic rotor identification Speed is limited to the maximum safe speed of the identified rotor If the system identifies a rotor different than the one entered by the user the system displays an error message and reduces the speed to the maximum
44. t exceed 75 noncondensing The centrifuge must have adequate air ventilation to ensure conformance to local require ments for vapors produced during operation M cm in 96 5 me 38 0 zen 24 5 Figure 2 1 Centrifuge Dimensions Installation ELECTRICAL REQUIREMENTS 208 V 60 Hz centrifuge 187 229 VAC 9 A 60 Hz 200 V 50 60 Hz centrifuge 180 220 VAC 10 A 50 60 Hz 230 V 50 Hz centrifuge 207 253 VAC 8 A 50 Hz To reduce the risk of electrical shock this centrifuge comes with a 2 5 m 8 ft three wire electrical cord attached to the power connector at the rear of the instrument and plug to connect the centrifuge to earth ground The plug for use with 208 VAC 60 Hz centrifuges is the NEMA 6 15P a NEMA 6 15R socket should be available In regions where the instrument is supplied with an unterminated cord a plug that meets local electrical and safety requirements must be supplied Contact your local Beckman Coulter Service Office for specific information regarding these requirements Make sure that the matching wall outlet receptacle is properly wired and earth grounded Check that the line voltage agrees with the voltage listed on the name rating plate affixed to the centrifuge Never use a three to two wire plug adapter Never use a two wire extension cord or a two wire non grounding type of multiple outlet r
45. t guarantee Speed Setspeed 2 2 200 to 10 200 rpm in 10 rpm increments Speed display actual rotor speed in 10 rpm increments or in RCF when selected Time Set Une cens rn ren to 99 hours 59 minutes or continuous hold Time display Timed run ose indicates run time remaining Continuous hold or pulse run indicates elapsed time Temperature Set temperature Allegra X I2R 10 to 40 C in 1 increments Allegra Al 2 2 a EN factory set at 20 C Temperature control after equilibration Allegra X 12R gt 4 yn ken b 0 2 C of set temperature Allegra X 12 22 2 22 2 C of the 20 C set temperature Temperature display after equilibration chamber temperature in 1 increments Ambient temperature range eese 10 to 35 C Ambient temperature range for optimum Operations ease taal EP ES UE 10 to 25 C Humidity restrictions 000 lt 75 noncondensing Acceleration sa Mee po US aan 10 acceleration rates Deceleration 2 4 cR LEE la iaa 11 deceleration rates Dimensions Wat hie us aset a as 76 2 cm 30 0 in D pth 534 esse tee rep Tbe NES plate RE Ets 62 2 cm 24 5 in Height ic 234 w kK We KERE A Aa l ek EN 34 3 cm 13 5 in Weight 2 cst pe Wie BOARS EIE EHE PI DES 121 kg 267 Ib Ventilation clearances sides and rear 71 6 cm 3 0 in Finishes Control panel ARK coated polystyrene c
46. t the door is closed START door LED will be lit Press ENTER SAVE then press START within 5 seconds 11 sTOP Wait for the set time to count down to zero or end the run by pressing STOP 12 DOOR When the rotor stops a tone sounds press DOOR to open the chamber door lift the door Operation PROGRAMMED RUN 1 POWER Turn the power switch on 2 DOOR Press DOOR to open the chamber door 3 Install the rotor according to the applicable rotor manual then close the chamber door 4 PROGRAM Press PROGRAM use the keypad or arrow keys to enter the required program number then press ENTER SAVE 5 ENTER SAVE Check that all parameters are correct and that the door is closed START door LED will be lit Press ENTER SAVE then press START within 5 seconds 6 STOP Wait for the run to end or end the run by pressing STOP 7 DOOR When the rotor stops a tone sounds press DOOR to open the chamber door lift the door PREPARATION Prepare the rotor for centrifugation as described in the applicable rotor manual For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration INSTALLING THE ROTOR The power must be turned on before you can unlock and open the chamber door To end a run for any reason do not turn the power switch off press STOP instead 3 3 Operation Action 1 Turn the power switch
47. te to the new speed Run time can be set for up to 99 hours and 59 minutes or for a hold continuous run The time display begins counting down when the rotor starts to spin For a timed run the run automatically terminates when the set time reaches zero and deceleration begins at the selected rate a tone sounds when the rotor has stopped For a hold run the elapsed time is displayed and the run continues until STOP is pressed Result If HOLD is displayed press TIME again to enter time Entered time appears on the display If you entered a number higher than 59 minutes the centrifuge automatically recalculates the time in hours and minutes HOLD appears on the display ENTERING RUN TEMPERATURE ALLEGRA X 12R ONLY I gt NOTE Temperature is factory set at 20 C on the Allegra X 12 Operation Run temperature can be set from 10 to 40 C If no value is entered the centrifuge selects the last entered temperature Action Result 1 Press TEMP The cursor and the temperature range 10 to 40 C appear 2 Use the keypad or arrow Entered temperature appears on the display If the entered tempera keys to enter required ture is outside the valid temperature range an error message will temperature appear and the input will be cleared Enter a valid temperature 3 Press the next run parameter key or ENTER SAVE ENTERING ACCELERATION AND DECELERATION RATES You can select one of ten acceleration rates an
48. ters are the same for programmed operation as those described above for manual operation CREATING A NEW PROGRAM Action Result 1 Press PROGRAM then use The program number flashes the keypad or arrow keys to select a program number that is not in use or one that you want to overwrite and press ENTER SAVE 2 Enter run parameters rotor If you entered an unacceptable parameter an error message will be speed time temperature displayed Press CE and enter an acceptable value then press the acceleration deceleration rate next run parameter key settings Press ENTER SAVE Action 3 When all parameters are entered press ENTER SAVE 4 Press ENTER SAVE START to run the programmed run RECALLING A PROGRAM Action 1 Press PROGRAM then use the keypad or arrow keys to enter the number of the program Press ENTER SAVE to recall a program or press PROGRAM to exit program mode 2 Press ENTER SAVE and START to begin the programmed run Operation Result The program is saved into memory and Program saved appears on the display The program number displayed will stop flashing If any parameters are then modified the program number will show a dash I gt NOTE If the PROGRAM key is pressed while the program number is flashing program mode is exited without saving entered parameters into program memory Result The program parameters are displayed if ENTER SAVE w
49. tes then press CE to clear message restart run If problem persists call Beckman Coulter Field Service Continued 4 3 Troubleshooting 4 4 Table 4 1 Diagnostic Message Chart continued Diagnostic Number Message Problem Result Recommended Action H1 H2 H4 Speed control problem Run shuts down with Call Beckman Coulter Field Service Speed error call maximum brake Service H3 H5 and H6 Speed control problem Run shuts down with Call Beckman Coulter Field Speed error door locks for 45 minutes call Service no brake door locks for 45 minutes Service H Imbalance see manual Rotor load is severely out of balance Run shuts down with maximum brake Make sure that tubes or bottles are balanced and loaded symmetrically in the rotor as described in the applicable rotor manual With swinging bucket rotors remove the buckets and clean and lubricate the pivot pin sockets where the buckets contact as described in the applicable rotor manual Unlubri cated pivot pin sockets can prevent the buckets from reaching horizontal position which can cause imbalance Press CE to clear message Rotor tie down is not properly fastened Run shuts down with maximum brake Make sure rotor lid is properly aligned and tie down T bar is securely fastened Press CE to clear message Centrifuge is misaligned tilted Run shuts down with maximum brake Level th
50. tops open Power not on Plug in the power cord turn power on Source power failure See RETRIEVING YOUR SAMPLE below Displays are Power not on Plug in the power cord turn power on blank Electrical failure Make sure the power cord is securely connected call Beckman Coulter Field Service Chamber does not reach selected temperature Centrifuge cannot maintain selected temperature for rotor in use at speed selected Precool or prewarm rotors before running at low or high temperatures Precool rotor chamber by running a 30 minute cycle at the required temperature with the speed set at 2000 rpm Allegra X 12R Make sure the air intake vent is clear f using multiwell plates for runs exceeding 2 hours in a warm and or humid environment speed reduction may be required to maintain low sample temperature Chamber icing Allegra X 12R Excess humidity in chamber Wipe out moisture before each run Condensation build up between runs Leave door open between runs Set temperature to a setting higher than ambient temperature Turn centrifuge power off 4 5 Troubleshooting Action 1 Turn the power off and disconnect the power cord from the main power source Make sure that the rotor is not spinning Locate the release device cap underneath the bottom center of the centrifuge not visible from the centrifuge front See Figure 4 1 Use a flat bl

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