Cole Parmer - Masterflex
Contents
1. Package of 3 S 1200 511 User Manual S 1200 512 Dust Cover S 1200 513 Fuse 3A quantity 1 size5 x 20 S 1200 520 Service Manual 16 Troubleshooting Table 4 Troubleshooting PROBLEM Instrument Inoperative Power indicator has no light Possible Cause Power cord not connected to outlet Solution Plug instrument in Dead Power outlet Wrong voltage setting Change to a different outlet Internal fuse blown or defective electronic Call an authorized service component engineer Instrument can No Cuvette Adapter in the Sample Cuvette Adapter must be in not set 100 T Compartment Sample Compartment to open 0 000A T can not be sample holder shutter Light beam blocked e Holder misaligned e Shutter Check sample holder Lamp is old or defective Replace lamp Lamp is off alignment Refer to Lamp Replacement instructions in this manual Defective electronic component Sample holder Call an authorized service engineer Remove Cuvette Adapter or set to 00 0 T test tube Sample holder shutter May be stuck open Close shutter Defective electronic component Call an authorized service engineer Incorrect Bubbles or particles in solution Check sample preparation and Transmittance to analytical procedure Absorbance Defective electronic component Call an authorized service correlation engineer Digital Display Concentration reading frozen Change MODE fro2. 1000 nm Cole Parmer 1200 1201 are ideal for measurements in the visible wavelength region of the electromagnetic spectrum Working Principle The spectrophotometer consists of five parts 1 Light Source Halogen Lamp to supply the light 2 A Monochromator to isolate the wavelength of interest and eliminate the unwanted second order radiation 3 A Sample Compartment to accommodate the sample solution 4 A Detector to receive and convert the transmitted light to an electrical signal 5 A Digital Display to show absorbance or transmittance Figure 1 illustrates the relationship between these parts 100 T Gees EISE a Se Monochromator Sample Compartment Detector Digital Display Figure 1 Block Diagram for the Spectrophotometer 4 In the spectrophotometer light from the Halogen Lamp is focused on the entrance slit of the Monochromator where the collimating mirror directs the beam onto the grating The grating disperses the light beam to produce the spectrum a portion of which is focused on the exit slit of the Monochromator by a collimating mirror From here the beam is passed to a Sample Compartment through one of the filters which helps to eliminate unwanted second order radiation from the diffraction grating Upon leaving the Sample Compartment the beam is passed to the silicon photodiode Detector and causes the Detector to produce an electrical signal that is shown on the Digital Display window Specifications Table 1 lists
3. it is likely that the protection has been impaired the spectrophotometer shall be made inoperative and be secured against any unintended operation The protection is likely to be impaired if for example the spectrophotometer e Shows visible damage e Fails to perform the intended measurements e Has been subjected to prolonged storage under unfavorable conditions 3 e Has been subjected to severe transport stresses Performance Carry out performance checks with particular reference to wavelength and absorbance accuracy to ensure that the spectrophotometer is working within its specification especially when making measurements of an important nature Performance checks are detailed in this manual Radio Interference For compliance with the EMC standards referred to in the EC Declaration of Conformity it is necessary that only shielded cables supplied by Cole Parmer are used when connecting the instrument to computers and accessories Introduction The newly redesigned Cole Parmer 1200 1201 Spectrophotometers are single beam general purpose instruments designed to meet the needs of the conventional laboratory Cole Parmer 1200 1201 is ideal for various applications such as Clinical Chemistry Biochemistry Petrochemistry Environmental Protection Food and Beverage Labs Water and Waste Water Labs and other fields of quality control Featuring a digital display of photometric result easy operation and wavelength range of 325 nm
4. or any electrical device that may generate high frequency fields Set the unit up in an area that is free of dust corrosive gases and strong vibrations 2 Remove any obstructions or materials that could hinder the flow of air under and around the instrument 3 Select either 230V or 125V on the Voltage Selector Switch on the bottom of the 1200 1201 shown in Figure 2 to match your local main voltage supply 4 Turn on your Cole Parmer 1200 1201 and allow it to warm up for 15 minutes before taking any readings Voltage Selector Switch Bottom of Model 1200 1201 Figure 2 Bottom of Cole Parmer 1200 1201 6 1200 and 1201 Spectrophotometer Operation Panel Mode Indicator Show the current measurement mode T Transmittance A Absorbance C Concentration and F Factor refer to Figure 3 LCD Display Mode 0A 100 T O T Print Enter Sample compartment Wavelength Knob Figure 3 Cole Parmer 1200 Spectrophotometer MODE Button There are four modes T mode is transmittance mode A mode is absorbance mode C mode is to measure unknown sample concentration through a standard solution F mode is to measure unknown value with a previously determined factor VVVV A OA 100 T Button Adjust Digital Display reading to 100 T or 0 000A when blank reference solution is in the Sample Compartment V O T Button When in T mode put S 1200 111 into the Cuvette Holder to block the optical light beam Press V button to adjust the D
5. the specification for both 1200 and 1201 Table 1 Specifications Model 1200 Model 1201 Wavelength Range 325 1000 nm Spectral Bandpass 5 nm Wavelength Accuracy 2nm Wavelength Repeatability 1nm Stray Radiant Energy lt 0 5 T at 340 and 400 nm Photometric Range 0 125 T 0 2 0 Abs 0 1999C 0 1999 Factor Photometric Accuracy 1 0 T Power Requirements 115 230 V 10 60 50 Hz switchable Sample Holder Single Test Tube Cuvette 4 poisiton Cuvette Holder Dimensions 415W x 320D x 175H mm 16 1 2 x13 x 6 3 4 inches Net Weight 6 5 kg 15 lbs Unpacking Instructions Carefully unpack the contents and check the materials against the following packing list to ensure that you have received everything in good condition Packing List Refer to Table 2 for the Packing List Table 2 Packing List Description Quantity Model 1200 Model 1201 Spectrophotometer 1 1 Dust Cover 1 1 10 mm Optical Square Cuvette 2 2 Test Tubes 1 2 inch Box of 12 Cuvette Cap 1 V type Tube Holder 8 to 25 mm 1 installed 10 mm Single Cuvette Holder 1 Black Block 1 1 4 position Cuvette Holder 1 installed Power Cord 1 1 User Manual 1 1 Installation 1 Place the instrument in a suitable location away from direct sunlight In order to have the best performance from your instrument keep it as far as possible from any strong magnetic or electrical fields
6. 105 Water jacketed Cuvette Holder with base and panel for temperature controlled application S 1200 101 S 1200 102 and S 1200 105 all consist of two parts the upper holder and the identical universal base S 1200 106 Universal base for V type holder Single Cuvette Holder and water jacketed Holder Follow the steps below to replace a 4 position Cuvette Holder Figure 5 with a V type Tube Holder Test Tube Holder The only tool needed is a standard Philips head screwdriver r gt Figure 4 Sample Holders for Model 1200 1201 8 Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Figure 5 4 position Cuvette Holder with Base Remove 4 position Cuvette Holder Rod Unscrew the 4 position Cuvette Holder Rod by turning the knob counterclockwise until it was removed from the Holder Gently slide the guide rod out of the Sample Compartment Locate the Front Screw of the Holder Base Open the cover of the Sample Compartment remove any cuvettes or any breakable items push in the Cuvette Holder Rod black knob all the way back the first position is into the light beam and locate the Front Screw of the 4 position Cuvette Holder shown in Figure 6 Remove Front Screw of the Holder Base Look over the Sample Compartment remove the Front Screw Remove Back Screw of the Holder Base Place your hand ON TOP of the 4 position Cuvette Holder and gently slide towards the front The back screw is then exposed and can be removed T
7. 32 printer item number S 1100 206 as an optional accessory It requires a 9 pin null modem connection cable The RS232 Printer Setting should be as follows e Baud Rate 9600 bps e Parity None e Data Bits 8 e Stop Bit 1 UNICO Application Software The UNICO Application Software VIS1200 is Windows based software designed to operate with Cole Parmer Spectrophotometer Model 1200 1201 1200 1201 uses its RS232C Interface connector to connect with a PC The software runs on a PC with 95 98 Me NT 2000 XP operating system installed The Software offers two additional analytical methods Standard Curve and Absorbance vs Time Kinetics It performs the following methods for analysis e Absorbance Transmittance Concentration measure the Absorbance Transmittance Concentration Standard or Concentration Factor at a single wavelength within the range of 325 1000 nm e Standard Curve create a calibration curve choice of 4 curve fits with up to 8 standard solutions at a single wavelength to determine concentrations of unknown samples e Absorbance vs Time Kinetics measure a sample s absorbance change over a selected period of time store the test results in data table display the results graphically and calculate the reaction rate within a given time interval 12 Refer to the 1200 Series Spectrophotometer Software User s Manual Version SS 1 22 for detail Maintenance Lamp Replacement 1 Turn off and unplug the instr
8. Cole Parmer 1200 SERIES SPECTROPHOTOMETER new version USER S MANUAL Cole Parmer Instruments Company 625 East Bunker Court Vernon Hills IL 60061 800 232 4340 Table of Contents General Information 3 Introduction nm 4 Working brinciple cece cece cece cee eeeeesesseceeeseceseeseesesenssssesessnnnes 4 Ge d E 5 Unpacking Instructions cece eee teeteteeeeeeseeetesneeeeeenesees 6 Je UE e E 6 1200 and 1201 Spectrophotometer Operation Panel 7 Changing Curette Holders 8 Basic Operation eae rnrrrrr rreren nn 10 Sample Preparation and Anahvsie 10 Concentration Modes sn eet Zen heelen ee nn 11 Factor MOG Ciena RS ent en A tne eh a ty seat aly 11 Output and Data Processing teseeeeeeeesen 12 LE 1 PO O E NA AAO 12 UNICO Application Software 12 EN ner E 13 Lamp SE E EE 13 Wavelength Calibration Check 13 Absorbance Accuracy Check 14 Stray LEAT ING Ci ss ra de ae 15 1200 and 1201 barteliet 16 Troubleshooting nc Re ee 17 General Information The spectrophotometer described in this manual is designed to be used by properly trained personnel in a suitable equipped laboratory For the correct and safe use of this spectrophotometer it is essential that laboratory personnel follow generally accepted safe procedures in addition to the safety precautions called for in this manual The inside of the power supply unit is a hazardous area and its cover should not be removed under any circumstances A
9. NY Servicing must be done by an authorized person Some of the chemicals you use in the spectrophotometer may be corrosive flammable radioactive toxic and or potentially infective Care should be taken to follow the normal laboratory procedures for handling chemicals and samples Please carefully read the Safety Electrical Warning Performance and Radio Interference instructions below Safety This spectrophotometer has been designed and tested in accordance with EN 61326 1 1997 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use standard EMC Requirements The spectrophotometer has been tested and supplied in a safe condition The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT 1974 Read the following before installing and using the instrument and its accessories Electrical Before switching on the spectrophotometer make sure it is set to the voltage of the local power supply see Installation section The main plug shall be inserted in a socket provided with a protective earth grounded contact The protective action must not be negated by the use of an extension cord without a protective conductor Warning Any interruption of the protective conductor inside or outside the spectrophotometer or disconnection of the protective earth terminal is likely to make the spectrophotometer dangerous Intentional interruption is prohibited Whenever
10. ake out 4 position Cuvette Holder and insert V type Tube Holder Simply take out the 4 position Cuvette Holder from the V type Tube Holder and insert the V type Tube Holder into it Install V type Tube Holder Locate the Front and Back Screws of the V type Tube Holder Base and fasten them e Standard 4 cells Figure 1 e Overhead View Front Figure 6 Changing Cuvette Holders Basic Operation Simple OPERATION INSTRUCTIONS are printed on the front panel of your Cole Parmer 1200 1201 Three Basic Operations Sample Preparation and Analysis Concentration Mode C and Factor Mode are shown in this section Sample Preparation and Analysis A Spectrophotometer Warm up and 0 T Check 1 Turn on the spectrophotometer by turning on the Power Switch IO Allow 15 minutes for the instrument to warm up 2 Even through the 0 T is pre set it may shift due to unexpected factors like temperature change To check 0 T simply insert the Black Block item number S 1200 111 used to check the O T into the Cuvette Holder and close the lid Select T mode the display should be 00 0 T If not press the O T button to set O T NOTE If you lose the Black Block you may use any opaque object to block the light beam to the detector to carry out O T Check 3 Select either the T Transmittance Mode or A Absorbance Mode by pressing the MODE button until the red light for T or A is on Sample Preparation Select the desired wavelengt
11. at might affect the results i e temperature bandpass weighing and diluting errors this method is less accurate and should only be used as a guide Reference Johnson E A Potassium Dichromate as an absorbance standard 14 PSG Bulletin 1967 No 17 page 505 1 Make up N 100 sulfuric acid as the solvent and use part of it to make a solution containing 120 0 5 mg l of potassium dichromate 2 Wash out a square cuvette with solvent and fill with solvent 3 Put the cuvette in the Cuvette Adapter then into the Sample Compartment and close the lid 4 Set the wavelength to 350 nm 5 Set the MODE button to A 6 Set the reading to 0 000A using the A button 7 Empty the cuvette Wash out with dichromate solution and fill with dichromate solution 8 Put the cuvette in the Cuvette Adapter then into the Sample Compartment and close the lid 9 Read the absorbance of the standard from the Digital Display window The value should be 1 288 0 04 A Refer to the notes above when interpreting the result Stray Light Check Specification Less than 0 5 T at 340 nm by ASTM E 387 A good indication as to whether the stray light level is within specification may be obtained as follows 1 2 3 Set the wavelength to 340 nm Set the MODE button to T With the square Cuvette Adapter in the Sample Compartment but no cuvette close the lid and press the A button to set the display to 100 0 Remove the Cuvette Adapter from
12. e adapter and insert the Didymium filter into it Place it in the Sample Compartment again and close the lid Record the Absorbance reading on the Digital Display window Advance the wavelength setting by 1nm and repeat step 2 to 5 Repeat step 6 until the wavelength setting reaches 815 nm Look for the maximum absorbance reading obtained and this should be found within 805 809 nm The wavelength accuracy of the 1200 is 2 nm 9 If a middle wavelength check is desired set the wavelength to 522 nm optional 10 Make sure the Cuvette Adapter is empty and place it in the Sample Compartment Close the lid 11 Set zero Abs by pressing the button Wait a few seconds when the display flashes The reading should be 0 000A If not repeat step 11 12 Remove the Cuvette Adapter and insert the Didymium filter into it Place it in the Sample Compartment and close the lid 13 Record the absorbance reading on the Digital Display window 14 Advance the wavelength setting by 1 nm and repeat step 10 to 13 15 Repeat step 14 until the wavelength setting reaches 536 nm Again look for the maximum absorbance reading It should be within 527 531 nm gt A Oe TEDN Absorbance Accuracy Check Specification 1 at 1A The absorbance accuracy should be checked against a set of neutral density filters accurately calibrated to the NIST standards An alternative method using potassium dichromate is described below Due to the many factors th
13. ecific Transmittance values are not important as you are only looking for the wavelength where the minimum Transmittance maximum Absorbance occurs Note If you calibration filter has a certified peak valley curve attached please use the peaks on the curve to verify the instrument Holmium Oxide Filter Method 1 Turn instrument on and allow it to warm up for 15 minutes 2 Select the A mode 3 Set the wavelength to 350 nm 4 Make sure the Cuvette Adapter is empty and place it in the Sample Compartment Close the lid 5 Set zero Absorbance by pressing the A button Wait a few seconds when the display 13 flashes The reading should be 0 000A If not repeat step 5 Remove the Cuvette Adapter and insert the Holmium filter into it Place it in the Sample Compartment again and close the lid Record the Absorbance reading on the Digital Display window Advance the wavelength setting by 1 nm and repeat step 4 to 7 Repeat step 8 until the wavelength setting reaches 370 nm 0 Look for the maximum absorbance reading obtained and this should be found within 359 363 nm The wavelength accuracy of the 1200 is 2 nm D Se te Filter Method Set the Wavelength to 800 nm 2 Make sure the cuvette adapter is empty and place it in the Sample Compartment Close the lid 3 Set zero Abs by pressing the button Wait a few seconds when the display flashes The reading should then be 0 000A If not repeat step 3 Remove the cuvett
14. h by turning the WAVELENGTH control knob Make a blank reference solution by filling a clean square cuvette or test tube half full with distilled de ionized water or other specified solvent Wipe the cuvette with tissue to remove the fingerprints or droplets of liquid 6 Insert the blank cuvette into Sample Compartment and close the cover 7 Set 0 000A or 100 T with the 0A 100 T button akg NOTE This step fulfills the instruction on the front of the spectrophotometer Set Full Scale 8 Remove the blank square cuvette or test tube Set it aside in case that you may need to adjust the 0A 100 T using A button later i e change the wavelength C Sample Analysis 9 Rinse a second square cuvette with a small amount of the sample solution to be tested Fill the square cuvette half full and wipe it 10 Put the sample cuvette in the Sample Compartment Close the cover 11 Read the T or A from the Digital Display window Remove the sample cuvette or test tube 12 If you are to test the same sample at other wavelengths repeat step 4 to 10 for each wavelength 13 For each new sample you analyze repeat step 3 to 11 10 Concentration Mode C is used for determining the concentration of unknown samples NOTE This method should only be used when the relationship between Absorbance and Concentration is known to be linear The concentration of the Standard solution used to calibrate the instrument should be higher than the m
15. igital Display reading to 00 0 T ENT PRINT Button When pressed At A and T modes send the displayed results to printer At C mode set the concentration value refer to Concentration Mode in Basic Operation section At F mode set the factor number and change to C mode refer to Factor Mode in Basic Operation section If you want to print the test results at C mode push the MODE button to the position when the LED light is off then push the ENT button If connected with a PC using UNICO Windows based Application Software the communication with the PC will be set up refer to the UNICO 1200 Series Spectrophotometer Software User s Manual Version SS 1 22 for details Sample Compartment Accept 10 mm test tube or 10 mm square cuvette with different cuvette holders The compartment can accept up to 50 mm pathlength cuvettes with optional holders WAVELENGTH Wavelength Control Knob Select desired wavelength in nanometers nm Wavelength Readout Window Display desired wavelength Changing Cuvette Holders There are five different Cuvette Holders Sample Holders refer to Figure 4 from left to right e S 1200 101 Test Tube V type Cuvette Holder with base for 8 25 mm Diameter Test Tubes e 1200 102 Single Square Cuvette Holder with base for 10 mm Cuvettes e 1200 103 4 poaition Cuvette Holder for 10 mm Square Cuvette e S 1200 104 Longpath 4 position Cuvette Holder for up to 50 mm rectangular Cuvettes e S 1200
16. m C to F T does not change regardless of sample concentration or A and back to C Restart measurement procedure Wrong wavelength setting Check sample procedure and wavelength setting Insufficient sample volume Fill cuvette with more samples Stray sample preparation vapors Prepare the sample away from the instrument Use proper ventilation Bubbles or particles in solution Check sample preparation and analytical procedure Defective electronic component or loose wiring Table 4 Troubleshooting continued Call an authorized service engineer 17 Instrument drift No sufficient warm up time Check lamp has been properly and noise Significant temperature change installed or has moved during Lamp not adjusted properly transit Refer to Lamp Replacement instructions in this manual Lamp old or defective Replace with a new lamp Sample Holder Misaligned Refer to Lamp Replacement instructions in this manual Unstable power supply Call an authorized service Defective or dirty detector or defective engineer electronic component Incorrect Insufficient sample volume Fill cuvette with more samples readings Wrong wavelength setting Check analytical procedure obtained Failed to blank OA 100 T and wavelength setting Check Failed to set O T wavelength accuracy according to procedure in this manual Stray sample preparation vapors Prepare sample away from instr
17. nc and Abs vs Time Includes S 2100 226 cable Accessories S 1200 101 Test Tube V type Cuvette Holder with base for 8 25 mm diameter Test Tubes S 1200 102 Single Square Cuvette Holder with base for 10 mm Cuvettes S 1200 103 4 position Cuvette Holder for 10 mm Square Cuvette S 1200 104 Longpath 4 position Cuvette Holder for up to 50 mm rectangular Cuvettes S 1200P 105 Water jacketed Cuvette Holder with base and panel for temperature controlled application S 1200 106 Universal base for V type holder Single Cuvette Holder and water jacketed Holder S 1200 111 Black Block to set O T S 2100 115 Holmium Oxide Filter 10 mm x 10 mm x 45 mm S 2100 116 Didymium Filter 10 mm x 10 mm x 45 mm Output Devices S 1100 206 Printer requires S 1100 207 cable S 1100 207 Printer Cable male 25 pin to female female Null Modem S 2100 226 RS 232C cable 9 pin to 9 pin female Female Null Modem Glassware S 90 301 Test Tube Cuvettes 10 mm diameter 12 pcs S 90 302P 100 Disposable Cuvette Polystyrene 10 mm pathlength 100 pcs S 90 302P 500 Disposable Cuvette Polystyrene 10 mm pathlength 500 pcs S 90 304G Square Cuvettes Optical Glass Set of 2 S 90 305P Disposable semi micro Cuvette Polystyrene 10 mm pathlength 500 pcs Misc and Replacement Items S 1100 101 Experiment manual includes safety in the lab instructor guide S 1100 505 Tungsten Halogen Lamps 6V 10W G4 type S 1100 508 Printer Paper
18. ost concentrated sample Select the desired wavelength by turning the wavelength control knob Using the MODE button select A mode Insert the cuvette containing the blank solution Set 0 000A with the button Using the MODE button select C mode Insert a cuvette containing a standard solution of known concentration in the Sample Compartment and set the Digital Display window to be the value of the standard by using the and V buttons 7 Press the ENT button OSV PIN NOTE If the reading changes the factor required is too high i e gt 1999 to be displayed In this case divide the concentration by 10 re select the C mode by successive presses on the MODE button cycling through the F T and A modes and follow step 2 above to set the concentration of the standard to the reduced value 8 With the standard concentration set determine the concentration values of samples with unknown concentration by inserting the sample cuvette into the Sample Compartment and reading the value direct from the Digital Display 9 To read the value of the multiplier used to convert Abs to Concentration after measuring all the samples change the mode to F and read the multiplier from the display Keep a record of this value for future use Operational Note if the MODE is changed to read F or A and the Concentration C reading is frozen re start at step 1 Factor Mode This is a special mode for measuring concentration values of unkno
19. the Sample Compartment and close the lid Make a note of the reading that should be at or near 0 00 Prepare a solution containing 50 mg l of sodium nitrite NaNOz in distilled water and fill a square cuvette with this solution Insert the cuvette into the Cuvette Adapter place it in the Sample Compartment and close the lid The display should read lt 0 5 T If the reading obtained in step 4 is greater than 0 00 it should be subtracted from the displayed reading to give the correct reading for the stray light value 1200 and 1201 Parts List 15 Table 3 1200 and 1201 Parts List Item Number Description S 1200 Model 1200 new Spectrophotometer 5 nm Bandpass Wavelength range 325 1000 nm Voltage preset at 110V Complete with 10 mm single Square Cuvette Holder V type Holder for 5 10 mm round tube a pair of Glass Cuvettes 12 pcs of 10 mm Test Tubes RS 232C Port Dust Cover User Manual S 1200 E Same as Model 1200 but preset at 220 V S 1201 Model 1201 new Spectrophotometer 5 nm Bandpass Wavelength range 325 1000 nm Voltage preset at 110V Complete with 4 poaition Cuvette Holder for 10 mm Square Cuvettes a pair of Glass Cuvettes RS 232C Port Dust Cover User Manual S 1201 E Same as Model 1201 but preset at 220 V Software S 1200 401 UNICO Application Software for PC s Window s 95 98 or above Required Programs include Standard Curve Abs T Co
20. ument 2 Remove the lamp access door at the back of the instrument by removing the four set screws 3 Unplug the lamp from the white connector Insert the new lamp pushing it in as far as it will go Replacement lamp Item Number S 1200 505 6V 10W G4 type CAUTION DO NOT HANDLE THE LAMP WITH BARE FINGERS USE TISSUE OR CLOTH WHEN HANDLING LAMP 4 Turn on the instrument Set the wavelength at 340 nm insert a test tube or an empty cuvette with cuvette holder and blank the instrument If the energy is low adjust the lamp by pulling or pushing it so that the light beam is focused on the entrance slit of the monochromator Since the lamp socket is pre aligned there will be minimum if any adjustment required 5 Re install the lamp access door Wavelength Calibration Check Normally the Cole Parmer 1200 Series spectrophotometer retains its wavelength calibration indefinitely However if the instrument receives a severe shock or is abused use the following methods to check wavelength calibration Please note that this test requires the Didymium filter item number S 2100 116 or the Holmium Oxide filter item number S 2100 115 In the filter method the didymium filter has two distinct absorbance peaks at 529 nm and 807 nm The Holmium filter has a distinct peak at 361 nm When the instrument is calibrated properly you will find minimum Transmittance maximum Absorbance at the range 2 nm from these peaks Note that the sp
21. ument Use proper ventilation Bubbles or particles in solution Check sample preparation and analytical procedure Instrument out of electronic calibration Call an authorized service engineer 18
22. wn samples using a previously determined factor to convert absorbance readings to concentration 1 After setting the wavelength and setting zero Abs on the blank solution using the MODE button select F mode 2 Insert a cuvette containing a sample 3 Using the and V buttons set the Digital Display window to the desired value of the multiplier 4 Press the ENT button The spectrophotometer switches to the C mode Operational Note If the Concentration of the sample is too high to be displayed the instrument will not switch to C mode when the ENT button is pressed Dilute the sample and multiply the concentration reading by the dilution factor to obtain the original sample 11 concentration If dilution is impossible or causes other problems you may divide the factor value by 10 or 100 and follow step 1 to 4 to enter the new factor value You need to calculate the sample concentration by multiplying readout with the multiple 10 or 100 5 Read the concentration value of the sample direct from the display 6 Insert a cuvette containing the next sample and read the result Repeat until all samples have been measured Operational Note if the MODE is changed to read A or T and the Concentration C reading is frozen re start at step 1 Output and Data Processing Printer Model 1200 1201 Model has a RS232C Interface connector that can be connected to any RS232 printers Cole Parmer provides RS2
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