SETIS™ user manual
Contents
1. media transfer silicone tube The first two pieces are then connected to the air filters provided IMPORTANT Connection is made at the opposite side of the IN side of the filter Please take a look at the IN sign engraved on the filter s body Air filters could remain connected to the silicone tube until the air filters are discarded Tubes could then be removed washed and reused with new air filters Air filters are packaged and sterilized inside any autoclave resistant container They could also be sterilized in plastic bags IMPORTANT For any of the previous options ventilation should be guarantied between the inside of the container bag and the sterilization chamber If ventilation is not allowed sterilization might not be efficient or filters could get damaged Media transfer silicone tubes are packaged in aluminum foil and sterilized in the autoclave VERVIT bvba Karnemelkstraat 2 4 9060 Zelzate Belgium S ET S it T 32499265783 info vervit be www vervit be Vervil BE0463 068 793 IBAN BE86 0017 2734 2250 BIC GEBABEBB Assembly at the Laminar Flow cabinet Sterile culture vessel is opened in the laminar flow always facing the airflow Plant material is then introduced as a bulk Close tight the vessel by screwing the cap avoiding air and or growth media leakage It is recommended to use the SETIS cap s holder to guaranty a proper tightness of the vessels Otherwise ware gloves to assure a good g2. shelf surface The bioreactor has the required inclination to allow self drainage followed the VERVIT bvba Karnemelkstraat 2 4 9060 Zelzate Belgium S l IS it T 32499265783 info vervit be www vervit be vervIi BE0463 068 793 IBAN BE86 0017 2734 2250 BIC GEBABEBB immersion phase This inclination can be altered by irregular and or wavy shelf surfaces Place the bioreactor with the front side where all connectors and caps are present facing the outside of the shelf corridor Connect both filters with their respective compressed air line Now is the bioreactor ready to operate Wes ry SETIS bioreactors are designed to be placed side by side and back to back without loosing any shelf space with the advantage of having all connectors and caps at the outside of the shelf T 32499265783 info vervit be www vervit be 5 VERVIT bvba Karnemelkstraat 2 4 9060 Zelzate Belgium vervit BE0463 068 793 IBAN BE86 0017 2734 2250 BIC GEBABEBB Installation SETIS racks are made to fit 16 SETIS bioreactors per shelf two rows of 8 A standard rack setup is composed of 4 shelves with a total of 64 SETIS bioreactors All required pneumatic connections are supplied with the SETIS rack In case users adapt their lab racks for SETIS we recommend connecting a maximum of 8 bioreactors per row and a maximum of 4 rows in parallel per Control Point In total a maximum of 32 SETIS biore
3. to the laminar flow and place it vertically Turn the culture vessel very slowly towards its vertical position to avoid plant material displacement Clean the connectors from old media vessel with disinfection solution Disconnect the media transfer silicone tube at the side of the media connector of the media vessel Discard then this media vessel with old growth media Bring the new media vessel close to the culture vessel and remove the aluminium foil from its media connector Then reconnect the media transfer silicone tube to the media connector of the new media vessel Remove aluminium foil from the air connector of the new media vessel and place a new air filter via the silicone tube IMPORTANT Avoid touching the connectors and edge of the silicone tube with your hands If desired air filter from the old media vessel could be transferred and reused in the new media vessel At the shelf The topside of the media vessel is complementary to the bottom side of the culture vessel Via these pins the culture vessel is stacked and sliding is avoided Place SETIS bioreactors vertically while transporting with a trolley otherwise growth media could enter through the air connector of the media vessel Use the handlers integrated at the bottom of both sides of the media vessel to carry the bioreactor with your hands Use your thumbs to hold the culture vessel as shown in the picture Place the bioreactors on a perfectly horizontal and hard
4. User manual SETIS bioreactor We hope giving enough and clear information in this manual guiding you through the first trials with SETIS bioreactors without any difficulty If you require more information do not hesitate to contact us Manual last update May 2015 Enjoy SETIS HN Parts of SETIS bioreactor culture vessel 1x SE CV6 media vessel 1x SE MV4 screw cap for culture vessel 1x SE C80 screw cap for media vessel 1x SE C50 silicone gasket for culture vessel s cap 1x SE SG80 silicone gasket for media vessel s cap 1x SE SG50 air filters 2x SE F50 silicone tube 6 9mm 1x to be further sectioned in three pieces see below SE ST6 Sterilization It is important to sterilize all components separated as described in this manual DO NOT sterilizes the bioreactor once assembled and with air filters connected Prepare growth media liquid and place it in the media vessel while standing vertical on its backside A maximum of 3 liters of growth media is recommended Bigger volumes could result in growth media entering into the air filter from the vessel and consequently damaging it Place the silicone gasket in the corresponding screw cap and close tight the vessel It is recommended to use the SETIS cap s holder see picture to guaranty a proper tightness of the vessels Otherwise ware gloves to assure a good grip on the caps while screwing VERVIT bvba Kar
5. actors per Control Point Based on SETIS Control unit IMPORTANT Design and calculate your compressed air supply based on the desired rack setup therefore avoiding bioreactor s malfunction SETIS bioreactor has been designed with a minimum height difference between the media connectors of both vessels Hence the air pressure required to perform an immersion is very low Working air pressure is between 0 1 0 3 BAR Preferably 0 1 BAR Higher pressures might result in vessel cracking hence leakages General remarks Working with liquid systems requires a careful handling under sterile conditions as contaminations may easily outgrow During the first trials with SETIS bioreactors perform slowly all activities under sterile conditions in order to reduce mistakes and unnecessary contaminations The Practice is the best method to become an expert handling SETIS bioreactors Silicone gaskets are designed to enter tight into the inner side of each cap This is to avoid that the silicone gasket falls from the cap once the vessel is opened under sterile conditions To place the silicone gasket correctly just place it into the cap and screw it to its corresponding vessel The edge of the vessel s neck will push the silicone gasket uniformly to its place IMPORTANT Avoid any loop in the silicone gasket prior screwing the caps This will result in vessel leakages Wash the vessels thoroughly using neutral detergent Aggres
6. nemelkstraat 2 4 9060 Zelzate Belgium S E TI S it T 32499265783 info vervit be www vervit be vervIi BE0463 068 793 IBAN BE86 0017 2734 2250 BIC GEBABEBB Cover both connectors air connector and media connector with aluminium foil Sterilize media vessel containing the growth media in the autoclave preferable 121 C 1 2 Atm for 20 25 min Place the vessels always vertically Once removed from the autoclave and still hot screw few millimeters again the cap to guaranty the tightness DO NOT do it if you have used the SETIS cap s holder to tight the caps prior sterilization Culture vessel is sterilized empty in the autoclave Place the silicone gasket in the corresponding screw cap and close tight the vessel It is recommended to use the SETIS cap s holder to guaranty a proper tightness of the vessels Otherwise ware gloves to assure a good grip on the caps while screwing VERVIT bvba Karnemelkstraat 2 4 9060 Zelzate Belgium S ET S rvit T 32499265783 info vervit be www vervit be ve l BE0463 068 793 IBAN BE86 0017 2734 2250 BIC GEBABEBB Cover both connectors air connector and media connector with aluminium foil Sterilize culture vessel in the autoclave preferable with autoclave program for materials at 121 125 C 1 2 1 4 Atm for 15 20 min Silicone tube 6 9mm provided with the bioreactor is cut in three pieces Cut two pieces of 8 cm length used to connect air filters and one piece of 18 cm
7. rip on the caps while screwing In case of sub culturing plant material from SETIS to SETIS it is then recommended to use plastic stretch foil to cover the cap neck join of the closed vessel This will reduce the accumulation of particles while bioreactor is standing on the shelves hence possible contaminations Once plant material is in the culture vessel place the culture vessel vertically on its backside Bring the media vessel besides it also vertically placed and face the top side of the media vessel with the bottom side of the culture vessel as indicated in the pictures Remove aluminium foil from the air connectors of both containers and place the air filters via the silicone tube IMPORTANT Avoid touching the connectors and edge of the silicone tube with your hands Once air filters are connected remove the aluminium foil from the media connectors and place the media transfer silicone tube Then is the bioreactor already assembled VERVIT bvba Karnemelkstraat 2 4 9060 Zelzate Belgium S ET S vervit T 32499265783 info vervit be www vervit be BE0463 068 793 IBAN BE86 0017 2734 2250 BIC GEBABEBB Change growth media SETIS bioreactors allows an easy fast and secure way of changing the growth media to plants in growth phase Follow these instructions to do so Fresh growth media is prepared and sterilized in a second media vessel using the same procedure as explained above Bring the SETIS bioreactor
8. sive detergents can induce cracks in the vessels body which will increase after several sterilization cycles Rinse them afterwards with clean water preferably demineralized water Avoid vessels to get in contact with solvents and oils Perform a periodic cleaning of your autoclave Liquid media is ideal for bacterial growth Endogenous bacteria which have been never detected in agar cultures could excessively grow in liquid cultures such as in SETIS bioreactors Control and diagnostic very good your starting materials against endogenous bacteria before you inoculate a SETIS bioreactor SETIS bioreactor is designed to place the culture vessel on top of the media vessel They will be solely connected via the media transfer silicone tube through both media connectors Make sure this connection is properly done without leakage Avoid twisting or bending the media transfer silicone tube VERVIT bvba Karnemelkstraat 2 4 9060 Zelzate Belgium S E S it T 32499265783 info vervit be www vervit be vervVIi BE0463 068 793 IBAN BE86 0017 2734 2250 BIC GEBABEBB
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