user manual - MBS
Contents
1. M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 14 of 20 MBS HACCP amp WATER EASY TEST salite USER MANUAL BASIC SYSTEM noLogieni 2013 FEBRUARY 1 2 4 Notes on microbiological analysis Foods with particular physical chemical characteristics eg low specific weight strong acidity alkalinity high viscosity marked coloration may interfere with the analysis in a case specific way In these circumstances it is recommend to dilute 1g or 1ml of sample in 10ml of suitable sterile solvent peptone water distilled water phisiological solution and to analyze 1ml of the solution Note that it is not necessary to adhere exactly to the amount indicated since the variability of microbiological statistical analysis is about 60 70 If after dilution the sample is not completely dissolved or forms flakes it is necessary to take 1g and proceed as described above After analysis it is possible to identify possible initial contamination taking into account the dilution used as required by reference methods ISO DIS 7954 e Example 1 Food Salad Type of analysis Escherichia coli CO A02 Dilution factor 10 diluting about 1g of food in 10ml of solvent Result analysis 1 4836E02 CFU g Real contamination 1 436E03 CFU g i e 1 436E02 x 10 dilution factor Products contain2. 20 MICRO OGICAL 2013 FEBRUARY MBS HACCP amp WATER EASY TEST USER MANUAL BASIC SYSTEM Step 3 Start analysis Open the previously prepared reaction vial Insert the swab into reaction vial If necessary fill in the Quality Control Sheet Place the vial in the incubator thermostat Note you can reseal the vial containing the neutralizing solution with the cap of reaction vial before disposal Step 4 Control of the analysis result You only need to check the color of the vial only once after a set number of hours depending on the type of analysis This number of hours does not depend only on the type of analysis but includes the operational limit of acceptable bacterial load in the sample the generally accepted limits for the type of sample taken by law are shown by way of illustration on the Quality Control Sheet Compare the color of the vial with the correlation table set out in paragraph 1 3 or in Quality Control Sheet The analysis result is positive if and only if occurs a complete color change of the vial content lf you have started the compilation of the Quality Control Sheet fill it in with the result of the analysis In the case of analysis for detection of E coli and only if the result is positive bright yellow vial you can perform a test to confirm the presence of E coll adding 3 5 drops of Kovacs reagent to the vial at the end of the analysis before the sterilization lf there is E coli with
3. Note to make the test for confirmation of E coli open the vial carefully avoiding contamination with the liquid contained in it because if the color is tacked the vial will contain a significant number of bacteria and it is therefore preferable to use disposable gloves even non sterile for the operation of opening the vial If you come in contact with the liquid wash the affected area immediately and thoroughly with antibacterial soap or normal soap and then with a disinfectant Step 5 Post analysis sterilization e Without opening the vial firmly press the top of the cap and shake for about 10 seconds The addition of the sterilizing agent can cause a color change After 5 10 minutes the contents of the vial are completely sterilized Note when the sterilization of the microorganisms are destroyed the reagents are rendered inert and the vial can be safely disposed of as a sanitary waste non hazardous according to the D M of 25 5 89 Such waste after sterilization can be disposed of in the same way as drugs which have expired M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 8 of 20 MBS HACCP amp WATER EASY TEST salite USER MANUAL BASIC SYSTEM BIOLOGICAL 2013 FEBRUARY 5 URNE 1 2 2 Liquid sample Step 1 Opening e Open the vial taking care to flip the cap s
4. amp WATER EASY TEST USER MANUAL BASIC SYSTEM OGICAL 2013 FEBRUARY Available reagents for the selective search of the following microorganisms 9 ad PO U RMDM Total Viable Count CBT A01 Coliforms Totals and E coli CO A02 Enterobacteriaceae EB A03 Staphylococcus aureus SP A04 Pseudomonas aeruginosa PAO A05 Salmonella spp SL A06 Listeria spp LY A07 Enterococcus faecalis EF A09 Yeasts Saccharomyces spp SC A11 M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 4 of 20 MBS HACCP amp WATER EASY TEST ie USER MANUAL BASIC SYSTEM OLOGICAL 2013 FEBRUARY 1 2 Howto use The kit comes in a pack containing all the material for the analysis the reaction vial vial and a vial of distilled water vial of water To perform the analysis you must also have a bacteriology thermostatic incubator programmable to 30 37 or 44 C Before handling the vials and proceeding with the analysis a thorough hand washing is recommended Furthermore you should follow the current regulations for the sampling procedures and the instructions given in the following paragraphs The performance analysis can be summarized into 5 phases opening insertion of the sample early analysis order analysis and sterilization It also differs by type
5. gloves even non sterile for the operation of opening the vial If you come in contact with the liquid wash the affected area immediately and thoroughly with antibacterial soap or normal soap and then with a disinfectant Step 5 Post analysis sterilization Without opening the vial firmly press the top of the cap and shake for about 10 seconds The addition of the sterilizing agent can cause a color change After 5 10 minutes the contents of the vial are completely M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 10 of 20 MBS HACCP amp WATER EASY TEST USER MANUAL BASIC SYSTEM BIOLOGICAL 2013 FEBRUARY sterilized Note when the sterilization of the microorganisms are destroyed the reagents are rendered inert and the vial can be safely disposed of as a sanitary waste non hazardous according to the D M of 25 5 89 Such waste after sterilization can be disposed of in the same way as drugs which have expired M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 11 of 20 MBS HACCP amp WATER EASY TEST salite USER MANUAL BASIC SYSTEM ni OLOGICAL 201 3 FEBRUARY SURWE 1 2 3 Surface analysis Step 1 Preparation of
6. of sample to be analyzed solid sample liquid sample or surface analysis M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 5 of 20 MBS HACCP amp WATER EASY TEST salite USER MANUAL BASIC SYSTEM BIOLOGICAL 2013 FEBRUARY 5 URNE 1 2 1 Solid sample Step 1 Opening e Open the vial taking care to flip the cap so that the inner surface does not come into contact with the surface to avoid contamination Step 2 Start analysis e Open the vial of water supplied with the reaction vial and insert the entire contents of the vial itself e Mix by inverting the vial several times to completely dissolve the reagent twenty seconds Note the vial grows 2 liquid phases the upper one consisting of white transparent oil does not change during the analysis formed at the bottom of the vial from the water with which the reagent is dissolved The color of the second phase will develop fully within 15 20 minutes from the dissolution of the reagent and that is what must be observed for the evaluation of the results of the analysis Note when using the MBS MR device to determine the Total Viable Count CBT wait at least 10 minutes from the dissolution of the reagent before inserting into the vial the sample to analyze Step 3 Inserting the sample e Take a small aliquot of the fo
7. the reaction vial e Open the vial of water supplied with the vial for analysis and insert the entire contents of the reaction vial itself e Mix by inverting the vial several times to completely dissolve the reagent twenty seconds Note the vial will grow in 2 liquid phases the upper one consisting of white transparent oil does not change during the analysis formed at the bottom of the vial from the water with which the reagent is dissolved The color of the second phase will develop fully within 15 20 minutes from the dissolution of the reagent and that is what must be observed for the evaluation of the results of the analysis Note when using the MBS MR device to determine the Total Viable Count CBT wait at least 10 minutes from the dissolution of the reagent before inserting into the vial the sample to analyze Note after dissolution of the reagent it is advisable to unscrew the reaction vial cap of analysis to facilitate subsequent operations Step 2 Sampling e Open the cotton swab vial TM A14 containing the neutralizing solution e Rub the swab on the surface trying to cover a square area of about 10 cm per side Note do not re insert the swab into the vial containing the neutralizing solution M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 12 of
8. 364 www emmebiesse net info emmebiesse net page 17 of 20 MBS HACCP amp WATER EASY TEST salita USER MANUAL BASIC SYSTEM BIOLOGICAL 2013 FEBRUARY SURVEY 1 3 3 E coli survey CO A02 Rod shaped aerobic Gram negative non spore forming cytochrome oxidase negative microorganism fermenting lactose with production of acids in the presence of bile salts or other surfactants at temperature of 44 C produce indole from tryptophan ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE CO A02 E coli MBS MICRO BIOLOGICAL SURVEY COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS positive NEGATIVE a io COLOR OF ANALYSIS AT END 30 HOURS TIME OF COLOR Lui cal ca si EQUATION 7 eis dica a aa x time in hours cru ormi gt CFU g o CFU ml 10 3 024 CONTAMINATION 5 i ii 10 i i i 1 i CFU cm 10f 92441 100 1 3 4 Enterobacteriaceae survey EB A04 Gram negative microorganisms aerobic anaerobic facultative non spore forming oxidase negative ferment glucose and lactose with gas production reduce nitrate and are negative for oxidase test ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE EB A03 Enterobactenaceae MBS MICRO BIOLOGICAL SURVEY 37 C COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS TIME OF ANALYSIS TIME OF COLOR CHANGE Reti E ET 10 00 13 00 16 00 19 00 2200 gt 2400 EOUATIOH h minute x time in hours Sa
9. ACCP amp WATER EASY TEST USER MANUAL BASIC SYSTEM Step 4 Control of the analysis result You only need to check the color of the vial once after a set number of hours depending on the type of analysis This number of hours does not depend only on the type of analysis but includes the operational limit of acceptable bacterial load in the sample the generally accepted limits for the type of sample taken by law are shown by way of illustration on the Quality Control Sheets Compare the color of the vial with the correlation table set out in paragraph 1 3 or in Quality Control Sheet The analysis result is positive if and only if occurs a complete color change of the vial content lf you have started the compilation of the Quality Control Sheet fill it in with the result of the analysis In the case of analysis for detection of E coli and only if the results are positive bright yellow vial you can perform a test to confirm the presence of E coli adding 3 5 drops of Kovacs reagent to the vial at the end of the analysis before the sterilization If there is E coll within a few seconds you will see the development of a small surface layer of purple red liquid Note to make the test for confirmation of E coli open the vial carefully avoiding contamination with the liquid contained in it because if the color is tacked the vial will contain a significant number of bacteria and it is therefore preferable to use disposable
10. Gram negative aerobic anaerobic facultative enterobacteria able to ferment mannitol Catalase positive produce hydrogen sulfide reduce nitrate to nitrite ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE SL A06 Salmonella spp MBS MICRO BIOLOGICAL SURVEY COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS POSITIVE NEGATIVE COLOR OF ANALYSIS AT END 32 HOURS TIME OF COLOR CHANGE EJEA 13 30 18 00 23 00 29 00 gt 32 00 m a SES e e e e e e a om CONTAMINATION i l z 1 3 8 Listeria spp survey LY A07 Gram positive non spore forming facultative anaerobic microorganisms resistant to many antibiotics Grow at pH between 5 and 9 and in the presence of NaCl to 10 Catalase positive oxidase negative do not hydrolyze urea gelatin and casein Do not reduce nitrates do not produce indole and hydrogen sulfide ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE LY AD7 Listeria spp MBS MICRO BIOLOGICAL SURVEY COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS COLOR OF ANALYSIS AT END l 36 HOURS TIME OF COLOR line 1200 1630 2030 2500 2900 3300 gt 3600 A ho minute x time in hours CONTAMINATION rien Ce owe pe ee CFUlem 1007 02 400 M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 19
11. MBS MBS HACCP amp WATER EASY TEST MICRO USER MANUAL BASIC SYSTEM 2013 FEBRUARY BIOLOGICAL SURVEY MBS HACCP amp WATER EASY TEST BASIC SYSTEM USER MANUAL M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net MBS HACCP amp WATER EASY TEST salita USER MANUAL BASIC SYSTEM BIOLOGICAL 2013 FEBRUARY 5 URNE TABLE OF CONTENTS 1 1 Introduction 3 1 2 How to use 5 1 2 1 Solid sample 6 1 2 2 Liquid sample 9 1 2 3 Surface analysis 12 1 2 4 Notes on microbiological analysis 15 1 3 Color scales and correlation tables 17 1 3 1 Total Viable Count CBT A01 17 1 3 2 Coliforms survey CO A02 17 1 3 3 E coli survey CO A02 18 1 3 4 Enterobacteriaceae survey EB A04 18 1 3 5 Staphylococcus aureus survey SP A04 18 1 3 6 Pseudomonas aeruginosa survey PAO A05 19 1 3 7 Salmonella spp survey SL A06 19 1 3 8 Listeria spp survey LY A07 19 1 3 9 Enterococcus faecalis survey EF A09 20 1 3 10 Saccharomyces spp survey SC A11 20 M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 2 of 20 MBS HACCP amp WATER EASY TEST salite USER MANUAL BASIC SYSTEM noLogieni 2013 FEBRUARY 1 1 Introduction Dear User thank you for p
12. e to extrinsic causes Step 4 Control of the analysis results e You only need to check the color of the vial once after a set number of hours depending on the type of analysis This number of hours does not depend only on the type of analysis but includes the operational limit of acceptable bacterial load in the sample the generally accepted limits for the type of sample taken by law are shown by way of illustration on the Quality Control Sheets e Compare the color of the vial with the correlation table set out in paragraph 1 3 or in Quality Control Sheet The analysis result is positive if and only if occurs a complete color change of the vial content e lf you have started the compilation of the Quality Control Sheet fill it in with the result of the analysis e Inthe case of analysis for detection of E coli and only if the result is positive bright yellow vial you can perform a test to confirm the presence of E coll adding 3 5 drops of Kovacs reagent to the vial at the end of the analysis before the sterilization lf there is E coli within a few seconds you will see the development of a small surface layer of purple red liquid M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 7 of 20 MBS HACCP amp WATER EASY TEST so USER MANUAL BASIC SYSTEM OLOGICAL 2013 FEBRUARY
13. in a few seconds you will see the development of a small surface layer of purple red liquid Note to make the test for confirmation of E coli open the vial carefully avoiding contamination with the liquid contained in it because if the color is tacked the vial will contain a significant number of bacteria and it is therefore preferable to use disposable gloves even non sterile for the operation of opening the vial If you come in contact M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 13 of 20 MBS HACCP amp WATER EASY TEST so USER MANUAL BASIC SYSTEM i OLOGICAL 201 3 FEBRUARY with the liquid wash the affected area immediately and thoroughly with antibacterial soap or normal soap and then with a disinfectant Step 5 Post analysis sterilization e Without opening the vial firmly press the top of the cap and shake for about 10 seconds The addition of the sterilizing agent can cause a color change After 5 10 minutes the contents of the vial is completely sterilized Note with the sterilization of the microorganisms are destroyed the reagents are rendered inert and the vial can be safely disposed of as a sanitary waste non hazardous according to the D M of 25 5 89 Such waste after sterilization can be disposed of in the same way as drugs which have expired
14. ing food additives like preservatives acidity regulators antioxidants emulsifiers etc could influence the course of the analysis Therefore the most appropriate solution for each individual case must be sought or sample dilution must be carried out as described above e Example 2 Food Mayonnaise Type of Analysis Total Viable Count at 30 C CBT A01 M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 15 of 20 MBS HACCP amp WATER EASY TEST USER MANUAL BASIC SYSTEM 2013 FEBRUARY Dilution factor 10 diluting about 1g of food in 10ml of solvent Result analysis 2 583E04 CFU g Real contamination 2 583E05 CFU g i e 2 584E04 x 10 dilution factor M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 16 of 20 MBS HACCP amp WATER EASY TEST salite USER MANUAL BASIC SYSTEM BIOLOGICAL 2013 FEBRUARY SURVEY 1 3 Color scales and correlation tables As required by law the concentration of bacteria is expressed in CFU g or CFU ml or CFU cm Colony Forming Units respectively for the analysis of solid or liquid samples or surfaces by analogy with the results expressed with the traditional analysis even if the MBS method i
15. o that the inner surface does not come into contact with the surface to avoid contamination Step 2 Start analysis e Open the vial of water supplied with the reaction vial and insert the entire contents of the vial itself e Mix by inverting the vial several times to completely dissolve the reagent twenty seconds Note the vial grow in 2 liquid phases the upper one consisting of white oil transparent does not change during the analysis formed at the bottom of the vial from the water with which the reagent is dissolved The color of the second phase will develop fully within 15 20 minutes from the dissolution of the reagent and that is what must be observed for the evaluation of the results of the analysis Note when using the MBS MR device to determine the Total Viable Count CBT wait at least 10 minutes from the dissolution of the reagent before inserting into the vial the sample to analyze Step 3 Inserting the sample e Using a pasteur dropper sterile disposable Supplied on request draw a quantity of fluid to examine and introduce about 1 ml of that liquid into the vial e f necessary fill in the Quality Control Sheet e Place the vial in the incubator thermostat M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 9 of 20 MICRO OGICAL 2013 FEBRUARY MBS H
16. od about the volume of a grain of corn approximately corresponding to 1 g with a tool used during the processing of the food itself and insert it into the vial alternatively you can use sterile tweezers e f necessary fill in the Quality Control Sheet M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 6 of 20 MBS HACCP amp WATER EASY TEST so USER MANUAL BASIC SYSTEM OLOGICAL 2013 FEBRUARY e Place the vial in the incubator thermostat Note the size or the exact weight of the sample to be examined is not important However the sample must be reduced to a very small parts maximum size 2 3 mm Infact the statistical variability microbiological examination of food samples is such that no method of analysis including the reference method has a variability of less than 60 70 Therefore the analysis has the same intrinsic value if instead of 1 g into the vial are inserted 2 g or 0 5 g This variability is due both to the method of analysis and to the sample itself since these microorganisms often grow in grouped colonies and therefore are not dispersed evenly in the middle Note for inserting the sample into the vial we recommend using a tool used during the processing of the food itself since by so doing you will be able to detect any contamination of the food du
17. of 20 MBS HACCP amp WATER EASY TEST ke USER MANUAL BASIC SYSTEM BIOLOGICAL 2013 FEBRUARY SURVEY 1 3 9 Enterococcus faecalis survey EF A09 Gram positive fixed facultative anaerobes non hemolytic catalase negative microorganism fermenting glucose without gas production and hydrolyze esculin ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE EF A09 Enterococcus faecalis MBS MICRO BIOLOGICAL SURVEY COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS POSITIVE COLOR OF ANALYSIS AT END 24 HOURS TIME OF COLOR Fi CHANGE 10 00 1300 1600 1900 22 00 gt 2400 FA ho Minute x time in hours CONTAMINATION n o CFUfcm as 1 10 10 1 i CFU cm 10 1 9 421 100 Unicellular eukaryotic heterotrophic highly aerobic organisms growing in carbon rich substrates resistant to high concentrations of antibiotics and sulfonamides ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE COLOR OF ANALYSIS AT END TIME OF COLOR i CHANGE lt 600 60 13 00 2030 27 30 3500 4200 50 00 T ORR ho minute x time in hours eee pele te pete pete De Le ii SC A11 Saccharomyces spp M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 20 of 20
18. s based on the detection of bacterial metabolism and not on the replication of microorganisms The credits approximately correspond to individual bacterial cells 1 3 1 Total Viable Count CBT A01 Detection of aerobic or microaerophilic mesophilic microorganisms able to grow on complete media ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE CBT A01 Total Viable Count MBs MICRO BIOLOGICAL SURVEY COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS postive NEGATIVE COLOR OF ANALYSIS AT END 24 HOURS TIME OF COLOR it minute x time in hours cite gt 10 0 stele te pope CFU o CFU g 107 922 CONTAMINATION Filen CFUfem 10 0103 100 1 3 2 Coliforms survey CO A02 Rod shaped aerobic microorganisms Gram negative non spore forming cytochrome oxidase negative fermenting lactose with production of acids in the presence of bile salts or other surfactants ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE CO A02 Coliforms MBS MICRO BIOLOGICAL SURVEY 3T C COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS POSITIVE NEGATIVE I COLOR OF ANALYSIS AT END F 24 HOURS TIME OF COLOR EQ CHANGE 3 3 12 30 16 30 19 00 gt 24 00 x ne spammer e oe De e e e LIL remi CONTAMINATION l l M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040
19. tie a se oo se 0 0 orcum CONTAMINATION 1 3 5 Staphylococcus aureus survey SP A04 Gram positive cocci coagulase positive catalase positive fixed non spore forming facultative anaerobes fermenting mannitol and osmotolerant ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE SP A04 Staphylococcus aureus COLOR OF ANALYSIS AT START TIME OF COLOR EI z CHANGE 1100 2000 2930 3600 4330 4600 gt 4800 ee i minute x time in hours CONTAMINATION 4 n3 CFUem 10 10 un CFU em 1051 0 2 100 M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 18 of 20 MBS HACCP amp WATER EASY TEST salita USER MANUAL BASIC SYSTEM BIOLOGICAL 2013 FEBRUARY SURVEY 1 3 6 Pseudomonas aeruginosa survey PAO A05 Moveable non spore forming microorganism rod shaped Gram negative aerobic anaerobic facultative cytochrome oxidase and catalase positive produce pyocyanine ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE PAO A05 Pseudomonas aeruginosa MBS MICRO BIOLOGICAL SURVEY COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS POSITIVE NEGATIVE COLOR OF ANALYSIS AT END 24 HOURS TIME OF COLOR pl e e e e e e e p a f ees a CONTAMINATION i f 1 3 7 Salmonella spp survey SL A06
20. urchasing MBS HACCP amp WATER EASY TEST an innovative rapid colorimetric system to perform microbiological tests on food water and surfaces developed in collaboration with Roma Tre University The method of analysis is based on the observation of the change of color in the suspension formed in the analysis vial used when the test sample is added the suspension changes color turns if there are microorganisms the greater the amount of microorganisms the more rapid the change of color The main features of the MBS HACCP amp WATER EASY TEST are Speed analysis time from preparation to the achievement of results from 2 to 5 times less than traditional methods Ease of use anyone anywhere can do the analysis without the need for other reagents or special equipment Sensitivity you can detect even a single microorganism present in the sample Selectivity it can detect different species of microbial organisms to the experimental limit of 99 999 Cost the cost of each analysis turns out to be 2 to 4 times cheaper than traditional methods The MBS method has been validated according to ISO 16140 2003 Microbiology of food and animal feeding stuffs Protocol for the validation of alternative methods M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 3 of 20 MICRO MBS HACCP
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